LOCUS       DACDKJ010000001       128752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDKJ010000001 DACDKJ010000000
VERSION     DACDKJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 128752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 128752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..128752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.001"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..1535)
                     /locus_tag="IV328_000001"
     CDS             complement(12..1535)
                     /locus_tag="IV328_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8936174.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="IV328_000002"
     CDS             1787..2968
                     /locus_tag="IV328_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8936175.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IV328_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IV328_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8936176.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="IV328_000004"
     CDS             complement(5053..7764)
                     /locus_tag="IV328_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8936177.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="IV328_000005"
     CDS             complement(7774..8475)
                     /locus_tag="IV328_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936178.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="IV328_000006"
     CDS             8661..9260
                     /locus_tag="IV328_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8936179.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="IV328_000007"
     CDS             9341..10354
                     /locus_tag="IV328_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8936180.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="IV328_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="IV328_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8936181.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="IV328_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="IV328_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8936182.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="IV328_000010"
     CDS             11802..13175
                     /locus_tag="IV328_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8936183.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="IV328_000011"
     CDS             13532..15676
                     /locus_tag="IV328_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8936184.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="IV328_000012"
     CDS             complement(15728..16768)
                     /locus_tag="IV328_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8936185.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="IV328_000013"
     CDS             complement(17045..18847)
                     /locus_tag="IV328_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8936186.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="IV328_000014"
     CDS             complement(18861..19613)
                     /locus_tag="IV328_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936187.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="IV328_000015"
     CDS             complement(19622..19984)
                     /locus_tag="IV328_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8936188.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="IV328_000016"
     CDS             complement(20160..21032)
                     /locus_tag="IV328_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8936189.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="IV328_000017"
     CDS             complement(21058..21570)
                     /locus_tag="IV328_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8936190.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22538)
                     /locus_tag="IV328_000018"
     CDS             complement(21708..22538)
                     /locus_tag="IV328_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8936191.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(22554..23162)
                     /locus_tag="IV328_000019"
     CDS             complement(22554..23162)
                     /locus_tag="IV328_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8936192.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="IV328_000020"
     CDS             complement(23155..23472)
                     /locus_tag="IV328_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8936193.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="IV328_000021"
     CDS             complement(23628..24356)
                     /locus_tag="IV328_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8936194.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="IV328_000022"
     CDS             24515..25396
                     /locus_tag="IV328_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8936195.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="IV328_000023"
     CDS             complement(25633..26676)
                     /locus_tag="IV328_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8936196.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLYFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="IV328_000024"
     CDS             complement(26679..27347)
                     /locus_tag="IV328_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8936197.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="IV328_000025"
     CDS             27489..29825
                     /locus_tag="IV328_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8936198.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="IV328_000026"
     CDS             complement(29940..30251)
                     /locus_tag="IV328_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936199.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="IV328_000027"
     CDS             30432..31907
                     /locus_tag="IV328_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8936200.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="IV328_000028"
     CDS             31954..32538
                     /locus_tag="IV328_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8936201.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="IV328_000029"
     CDS             32540..32959
                     /locus_tag="IV328_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8936202.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="IV328_000030"
     CDS             complement(33240..33524)
                     /locus_tag="IV328_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8936203.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="IV328_000031"
     CDS             complement(33876..34586)
                     /locus_tag="IV328_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8936204.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="IV328_000032"
     CDS             complement(34583..36055)
                     /locus_tag="IV328_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8936205.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="IV328_000033"
     CDS             36193..36666
                     /locus_tag="IV328_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8936206.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="IV328_000034"
     CDS             complement(36819..37370)
                     /locus_tag="IV328_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8936207.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="IV328_000035"
     CDS             37475..38005
                     /locus_tag="IV328_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8936208.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="IV328_000036"
     CDS             complement(38488..38931)
                     /locus_tag="IV328_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8936209.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="IV328_000037"
     CDS             complement(38947..40107)
                     /locus_tag="IV328_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8936210.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="IV328_000038"
     CDS             40614..42314
                     /locus_tag="IV328_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936211.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="IV328_000039"
     CDS             complement(42416..43249)
                     /locus_tag="IV328_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8936212.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="IV328_000040"
     CDS             complement(43553..45718)
                     /locus_tag="IV328_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8936213.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="IV328_000041"
     CDS             45978..48272
                     /locus_tag="IV328_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8936214.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="IV328_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="IV328_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8936215.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="IV328_000043"
     CDS             48923..49930
                     /locus_tag="IV328_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8936216.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="IV328_000044"
     CDS             49952..50971
                     /locus_tag="IV328_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8936217.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="IV328_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="IV328_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8936218.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="IV328_000046"
     CDS             complement(52729..54150)
                     /locus_tag="IV328_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8936219.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="IV328_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="IV328_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8936220.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="IV328_000048"
     CDS             complement(56561..57817)
                     /locus_tag="IV328_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8936221.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            58397..59698
                     /locus_tag="IV328_000049"
     CDS             58397..59698
                     /locus_tag="IV328_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8936222.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="IV328_000050"
     CDS             complement(59767..60366)
                     /locus_tag="IV328_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8936223.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="IV328_000051"
     CDS             60620..61501
                     /locus_tag="IV328_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936224.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="IV328_000052"
     CDS             61514..63124
                     /locus_tag="IV328_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8936225.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="IV328_000053"
     CDS             complement(63201..63860)
                     /locus_tag="IV328_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8936226.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="IV328_000054"
     CDS             complement(64143..64808)
                     /locus_tag="IV328_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936227.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="IV328_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="IV328_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8936228.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66571)
                     /gene="dhaK"
                     /locus_tag="IV328_000056"
     CDS             complement(65588..66571)
                     /gene="dhaK"
                     /locus_tag="IV328_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8936229.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="IV328_000057"
     CDS             complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="IV328_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8936230.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="IV328_000058"
     CDS             67122..67442
                     /locus_tag="IV328_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8936231.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="IV328_000059"
     CDS             complement(67554..68423)
                     /locus_tag="IV328_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8936232.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69793)
                     /locus_tag="IV328_000060"
     CDS             complement(68519..69793)
                     /locus_tag="IV328_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8936233.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(69819..70328)
                     /locus_tag="IV328_000061"
     CDS             complement(69819..70328)
                     /locus_tag="IV328_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936234.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="IV328_000062"
     CDS             complement(70503..72638)
                     /locus_tag="IV328_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8936235.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="IV328_000063"
     CDS             complement(72721..74376)
                     /locus_tag="IV328_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8936236.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="IV328_000064"
     CDS             complement(74378..74692)
                     /locus_tag="IV328_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8936237.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="IV328_000065"
     CDS             74862..75122
                     /locus_tag="IV328_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8936238.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="IV328_000066"
     CDS             75136..75450
                     /locus_tag="IV328_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8936239.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="IV328_000067"
     CDS             complement(76180..76734)
                     /locus_tag="IV328_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8936240.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="IV328_000068"
     CDS             complement(76860..78788)
                     /locus_tag="IV328_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8936241.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="IV328_000069"
     CDS             complement(79094..79810)
                     /locus_tag="IV328_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8936242.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="IV328_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="IV328_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8936243.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="IV328_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="IV328_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8936244.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="IV328_000072"
     CDS             complement(83280..84740)
                     /locus_tag="IV328_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8936245.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="IV328_000073"
     CDS             complement(84730..86025)
                     /locus_tag="IV328_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8936246.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="IV328_000074"
     CDS             complement(86357..87034)
                     /locus_tag="IV328_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8936247.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="IV328_000075"
     CDS             complement(87064..88155)
                     /locus_tag="IV328_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8936248.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="IV328_000076"
     CDS             complement(88256..88690)
                     /locus_tag="IV328_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8936249.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="IV328_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="IV328_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8936250.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="IV328_000078"
     CDS             complement(90728..92623)
                     /locus_tag="IV328_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8936251.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="IV328_000079"
     CDS             complement(92681..94456)
                     /locus_tag="IV328_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8936252.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="IV328_000080"
     CDS             complement(94506..96002)
                     /locus_tag="IV328_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8936253.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="IV328_000081"
     CDS             complement(96196..97818)
                     /locus_tag="IV328_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8936254.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="IV328_000082"
     CDS             complement(98267..99580)
                     /locus_tag="IV328_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8936255.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="IV328_000083"
     CDS             complement(99825..100049)
                     /locus_tag="IV328_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936256.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100437)
                     /locus_tag="IV328_000084"
     CDS             complement(100069..100437)
                     /locus_tag="IV328_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8936257.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="IV328_000085"
     CDS             complement(100635..101345)
                     /locus_tag="IV328_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936258.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="IV328_000086"
     CDS             complement(101363..101860)
                     /locus_tag="IV328_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8936259.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="IV328_000087"
     CDS             complement(101897..102349)
                     /locus_tag="IV328_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8936260.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="IV328_000088"
     CDS             complement(102484..103089)
                     /locus_tag="IV328_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8936261.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="IV328_000089"
     CDS             complement(103539..104159)
                     /locus_tag="IV328_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8936262.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="IV328_000090"
     CDS             complement(104161..105030)
                     /locus_tag="IV328_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8936263.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="IV328_000091"
     CDS             complement(105053..105460)
                     /locus_tag="IV328_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8936264.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="IV328_000092"
     CDS             complement(105479..106768)
                     /locus_tag="IV328_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8936265.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="IV328_000093"
     CDS             complement(106795..107097)
                     /locus_tag="IV328_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8936266.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="IV328_000094"
     CDS             complement(107122..109215)
                     /locus_tag="IV328_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8936267.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="IV328_000095"
     CDS             complement(109704..110468)
                     /locus_tag="IV328_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8936268.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="IV328_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="IV328_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8936269.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="IV328_000097"
     CDS             complement(111473..112549)
                     /locus_tag="IV328_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8936270.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="IV328_000098"
     CDS             complement(112573..113166)
                     /locus_tag="IV328_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8936271.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="IV328_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="IV328_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8936272.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVILALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="IV328_000100"
     CDS             complement(114836..115135)
                     /locus_tag="IV328_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8936273.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="IV328_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="IV328_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8936274.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="IV328_000102"
     CDS             complement(116890..117801)
                     /locus_tag="IV328_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8936275.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="IV328_000103"
     CDS             117921..118778
                     /locus_tag="IV328_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8936276.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="IV328_000104"
     CDS             complement(118965..119327)
                     /locus_tag="IV328_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936277.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="IV328_000105"
     CDS             119673..121112
                     /locus_tag="IV328_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8936278.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="IV328_000106"
     CDS             complement(121119..123671)
                     /locus_tag="IV328_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8936279.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="IV328_000107"
     CDS             complement(123785..125116)
                     /locus_tag="IV328_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8936280.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="IV328_000108"
     CDS             complement(125082..126560)
                     /locus_tag="IV328_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8936281.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="IV328_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="IV328_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8936282.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        37651 a        26928 c        22529 g        41644 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaataca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggac ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaaggatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt ta
//
LOCUS       DACDKJ010000002       100948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDKJ010000002 DACDKJ010000000
VERSION     DACDKJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.002"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            211..603
                     /locus_tag="IV328_000110"
     CDS             211..603
                     /locus_tag="IV328_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8936283.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            631..1971
                     /gene="glnA"
                     /locus_tag="IV328_000111"
     CDS             631..1971
                     /gene="glnA"
                     /locus_tag="IV328_000111"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8936284.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3164..4774
                     /locus_tag="IV328_000112"
     CDS             3164..4774
                     /locus_tag="IV328_000112"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8936285.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5066..5935
                     /locus_tag="IV328_000113"
     CDS             5066..5935
                     /locus_tag="IV328_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8936286.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="IV328_000114"
     CDS             6100..7371
                     /locus_tag="IV328_000114"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8936287.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /locus_tag="IV328_000115"
     CDS             7368..8663
                     /locus_tag="IV328_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8936288.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="IV328_000116"
     CDS             8762..9025
                     /locus_tag="IV328_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8936289.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="IV328_000117"
     CDS             9470..9919
                     /locus_tag="IV328_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8936290.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="IV328_000118"
     CDS             9976..11394
                     /locus_tag="IV328_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8936291.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="IV328_000119"
     CDS             11394..12254
                     /locus_tag="IV328_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8936292.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="IV328_000120"
     CDS             12428..13201
                     /locus_tag="IV328_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8936293.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="IV328_000121"
     CDS             13348..13713
                     /locus_tag="IV328_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8936294.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="IV328_000122"
     CDS             complement(13861..15069)
                     /locus_tag="IV328_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8936295.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="IV328_000123"
     CDS             complement(15088..15366)
                     /locus_tag="IV328_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936296.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15559..16215
                     /locus_tag="IV328_000124"
     CDS             15559..16215
                     /locus_tag="IV328_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8936297.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16657..17454)
                     /locus_tag="IV328_000125"
     CDS             complement(16657..17454)
                     /locus_tag="IV328_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8936298.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="IV328_000126"
     CDS             complement(17451..18635)
                     /locus_tag="IV328_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8936299.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="IV328_000127"
     CDS             complement(18660..19907)
                     /locus_tag="IV328_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8936300.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="IV328_000128"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="IV328_000128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8936301.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="IV328_000129"
     CDS             21887..22201
                     /locus_tag="IV328_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8936302.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="IV328_000130"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="IV328_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8936303.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /locus_tag="IV328_000131"
     CDS             22886..23530
                     /locus_tag="IV328_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8936304.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="IV328_000132"
     CDS             23545..23874
                     /locus_tag="IV328_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936305.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="IV328_000133"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="IV328_000133"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8936306.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="IV328_000134"
     CDS             24862..25686
                     /locus_tag="IV328_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8936307.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="IV328_000135"
     CDS             25679..26026
                     /locus_tag="IV328_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8936308.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="IV328_000136"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="IV328_000136"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8936309.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="IV328_000137"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="IV328_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8936310.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="IV328_000138"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="IV328_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8936311.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="IV328_000139"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="IV328_000139"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8936312.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKNQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31683..32450
                     /locus_tag="IV328_000140"
     CDS             31683..32450
                     /locus_tag="IV328_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936313.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="IV328_000141"
     CDS             32440..34482
                     /locus_tag="IV328_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8936314.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34763..34912
                     /locus_tag="IV328_000142"
     CDS             34763..34912
                     /locus_tag="IV328_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8936315.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34923..36437
                     /gene="dltA"
                     /locus_tag="IV328_000143"
     CDS             34923..36437
                     /gene="dltA"
                     /locus_tag="IV328_000143"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8936316.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36434..37639
                     /gene="dltB"
                     /locus_tag="IV328_000144"
     CDS             36434..37639
                     /gene="dltB"
                     /locus_tag="IV328_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8936317.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37684..37917
                     /gene="dltC"
                     /locus_tag="IV328_000145"
     CDS             37684..37917
                     /gene="dltC"
                     /locus_tag="IV328_000145"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8936318.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37920..39188
                     /gene="dltD"
                     /locus_tag="IV328_000146"
     CDS             37920..39188
                     /gene="dltD"
                     /locus_tag="IV328_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8936319.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39329..40411
                     /locus_tag="IV328_000147"
     CDS             39329..40411
                     /locus_tag="IV328_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8936320.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40411..41214
                     /locus_tag="IV328_000148"
     CDS             40411..41214
                     /locus_tag="IV328_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8936321.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41284..42024
                     /locus_tag="IV328_000149"
     CDS             41284..42024
                     /locus_tag="IV328_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8936322.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42035..43039
                     /locus_tag="IV328_000150"
     CDS             42035..43039
                     /locus_tag="IV328_000150"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8936323.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43084..44928)
                     /locus_tag="IV328_000151"
     CDS             complement(43084..44928)
                     /locus_tag="IV328_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936324.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45140..45658
                     /locus_tag="IV328_000152"
     CDS             45140..45658
                     /locus_tag="IV328_000152"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8936325.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45786..46469
                     /locus_tag="IV328_000153"
     CDS             45786..46469
                     /locus_tag="IV328_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8936326.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46778..46851
                     /locus_tag="IV328_000154"
     tRNA            46778..46851
                     /locus_tag="IV328_000154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46812..46814,aa:Arg,seq:tct)
     gene            47010..47369
                     /locus_tag="IV328_000155"
     CDS             47010..47369
                     /locus_tag="IV328_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8936327.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47492..49123
                     /locus_tag="IV328_000156"
     CDS             47492..49123
                     /locus_tag="IV328_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8936328.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49185..49826)
                     /locus_tag="IV328_000157"
     CDS             complement(49185..49826)
                     /locus_tag="IV328_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8936329.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            50108..50416
                     /gene="rplU"
                     /locus_tag="IV328_000158"
     CDS             50108..50416
                     /gene="rplU"
                     /locus_tag="IV328_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8936330.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50443..50787
                     /locus_tag="IV328_000159"
     CDS             50443..50787
                     /locus_tag="IV328_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8936331.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50800..51093
                     /gene="rpmA"
                     /locus_tag="IV328_000160"
     CDS             50800..51093
                     /gene="rpmA"
                     /locus_tag="IV328_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8936332.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51500..54334
                     /locus_tag="IV328_000161"
     CDS             51500..54334
                     /locus_tag="IV328_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8936333.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            54525..55016
                     /locus_tag="IV328_000162"
     CDS             54525..55016
                     /locus_tag="IV328_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8936334.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55119..56108
                     /gene="manX"
                     /locus_tag="IV328_000163"
     CDS             55119..56108
                     /gene="manX"
                     /locus_tag="IV328_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8936335.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56141..56944
                     /locus_tag="IV328_000164"
     CDS             56141..56944
                     /locus_tag="IV328_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8936336.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56965..57879
                     /locus_tag="IV328_000165"
     CDS             56965..57879
                     /locus_tag="IV328_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8936337.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57962..58336
                     /locus_tag="IV328_000166"
     CDS             57962..58336
                     /locus_tag="IV328_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8936338.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58475..58720
                     /locus_tag="IV328_000167"
     CDS             58475..58720
                     /locus_tag="IV328_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8936339.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58892..59956
                     /locus_tag="IV328_000168"
     CDS             58892..59956
                     /locus_tag="IV328_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8936340.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60202..60627
                     /locus_tag="IV328_000169"
     CDS             60202..60627
                     /locus_tag="IV328_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8936341.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60620..61069
                     /gene="nusB"
                     /locus_tag="IV328_000170"
     CDS             60620..61069
                     /gene="nusB"
                     /locus_tag="IV328_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8936342.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61166..62020
                     /locus_tag="IV328_000171"
     CDS             61166..62020
                     /locus_tag="IV328_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8936343.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62021..63358
                     /locus_tag="IV328_000172"
     CDS             62021..63358
                     /locus_tag="IV328_000172"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8936344.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63363..63587
                     /locus_tag="IV328_000173"
     CDS             63363..63587
                     /locus_tag="IV328_000173"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8936345.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63589..64479
                     /locus_tag="IV328_000174"
     CDS             63589..64479
                     /locus_tag="IV328_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8936346.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64488..65306
                     /locus_tag="IV328_000175"
     CDS             64488..65306
                     /locus_tag="IV328_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8936347.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65413..65862
                     /locus_tag="IV328_000176"
     CDS             65413..65862
                     /locus_tag="IV328_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8936348.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65880..67562
                     /gene="recN"
                     /locus_tag="IV328_000177"
     CDS             65880..67562
                     /gene="recN"
                     /locus_tag="IV328_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8936349.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67617..67733)
                     /locus_tag="IV328_000178"
     CDS             complement(67617..67733)
                     /locus_tag="IV328_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8936350.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67806..68762)
                     /locus_tag="IV328_000179"
     CDS             complement(67806..68762)
                     /locus_tag="IV328_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8936351.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68933..69301
                     /locus_tag="IV328_000180"
     CDS             68933..69301
                     /locus_tag="IV328_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8936352.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69471..69902
                     /gene="mraZ"
                     /locus_tag="IV328_000181"
     CDS             69471..69902
                     /gene="mraZ"
                     /locus_tag="IV328_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8936353.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69923..70882
                     /gene="rsmH"
                     /locus_tag="IV328_000182"
     CDS             69923..70882
                     /gene="rsmH"
                     /locus_tag="IV328_000182"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8936354.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70887..71288
                     /gene="ftsL"
                     /locus_tag="IV328_000183"
     CDS             70887..71288
                     /gene="ftsL"
                     /locus_tag="IV328_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8936355.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71289..73481
                     /locus_tag="IV328_000184"
     CDS             71289..73481
                     /locus_tag="IV328_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8936356.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73507..74469
                     /locus_tag="IV328_000185"
     CDS             73507..74469
                     /locus_tag="IV328_000185"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8936357.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74483..75844
                     /locus_tag="IV328_000186"
     CDS             74483..75844
                     /locus_tag="IV328_000186"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8936358.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75870..76958
                     /gene="murG"
                     /locus_tag="IV328_000187"
     CDS             75870..76958
                     /gene="murG"
                     /locus_tag="IV328_000187"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8936359.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76973..78193
                     /locus_tag="IV328_000188"
     CDS             76973..78193
                     /locus_tag="IV328_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8936360.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78349..79674
                     /gene="ftsA"
                     /locus_tag="IV328_000189"
     CDS             78349..79674
                     /gene="ftsA"
                     /locus_tag="IV328_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8936361.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79697..80938
                     /gene="ftsZ"
                     /locus_tag="IV328_000190"
     CDS             79697..80938
                     /gene="ftsZ"
                     /locus_tag="IV328_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8936362.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80955..81632
                     /locus_tag="IV328_000191"
     CDS             80955..81632
                     /locus_tag="IV328_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8936363.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81646..82245
                     /locus_tag="IV328_000192"
     CDS             81646..82245
                     /locus_tag="IV328_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8936364.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82260..82547
                     /locus_tag="IV328_000193"
     CDS             82260..82547
                     /locus_tag="IV328_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8936365.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82596..83378
                     /locus_tag="IV328_000194"
     CDS             82596..83378
                     /locus_tag="IV328_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8936366.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83399..84106
                     /locus_tag="IV328_000195"
     CDS             83399..84106
                     /locus_tag="IV328_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8936367.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84368..87166
                     /gene="ileS"
                     /locus_tag="IV328_000196"
     CDS             84368..87166
                     /gene="ileS"
                     /locus_tag="IV328_000196"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8936368.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87215..88741)
                     /locus_tag="IV328_000197"
     CDS             complement(87215..88741)
                     /locus_tag="IV328_000197"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8936369.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88756..89403)
                     /locus_tag="IV328_000198"
     CDS             complement(88756..89403)
                     /locus_tag="IV328_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8936370.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89587..89916)
                     /locus_tag="IV328_000199"
     CDS             complement(89587..89916)
                     /locus_tag="IV328_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8936371.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90093..90821
                     /gene="sfsA"
                     /locus_tag="IV328_000200"
     CDS             90093..90821
                     /gene="sfsA"
                     /locus_tag="IV328_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8936372.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90837..91850
                     /locus_tag="IV328_000201"
     CDS             90837..91850
                     /locus_tag="IV328_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8936373.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91850..93127
                     /locus_tag="IV328_000202"
     CDS             91850..93127
                     /locus_tag="IV328_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8936374.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93190..95892
                     /locus_tag="IV328_000203"
     CDS             93190..95892
                     /locus_tag="IV328_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8936375.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96044..96361
                     /locus_tag="IV328_000204"
     CDS             96044..96361
                     /locus_tag="IV328_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8936376.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96391..96711
                     /locus_tag="IV328_000205"
     CDS             96391..96711
                     /locus_tag="IV328_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8936377.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96819..98279
                     /locus_tag="IV328_000206"
     CDS             96819..98279
                     /locus_tag="IV328_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8936378.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98347..98568
                     /locus_tag="IV328_000207"
     CDS             98347..98568
                     /locus_tag="IV328_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936379.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98599..98781
                     /locus_tag="IV328_000208"
     CDS             98599..98781
                     /locus_tag="IV328_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8936380.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98781..99194
                     /locus_tag="IV328_000209"
     CDS             98781..99194
                     /locus_tag="IV328_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8936381.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99317..100498
                     /locus_tag="IV328_000210"
     CDS             99317..100498
                     /locus_tag="IV328_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8936382.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        32983 a        17283 c        21422 g        29260 t
ORIGIN      
        1 ataaaaacac taataaagga tagtacagta taataaaaaa taaaaggtat ctggtacgaa
       61 aatcaactag ataccttttt tgtgttctta tttttttgtt attttataaa aaatagagat
      121 ctcatgttag aaaacctaac atatgcagtt gacaatattt tttttaattg gtacacttta
      181 gccaacttat aaaacgaaag gggacgtctg atgggagaaa aggaattgcg ccgatctatg
      241 tccgtatttc caattggtac agttatgaag ctgactgatt tgtctgctcg gcaaattcgc
      301 tattatgaag aacaagattt agttcatccg gaacgaagcg aaggaaaccg acgtatgtat
      361 tcgctgaatg atattgatat gttgcttgag attaaagact atctttcaga gggattgaat
      421 atggctggga tcaaacgtgt atacgaaatg gcacaggcga aaaaacaaga agcaaaaaac
      481 aagaagccgt tgacagatca tgacgttcga caaatttttc gtgatgagat tttggcgcaa
      541 ggtgggttaa gcaaaacagg tcattatcag tcgcaaggat tgcaaagaca attctttgac
      601 tgaataaatt aaaaaaggaa gagggatacc atggtaaaga aacagataac aggtgaagaa
      661 atcaaacgaa tcattgagga agaaaacgta cgatttttac gattgatgtt cacagacatc
      721 ttaggtacga tcaaaaatgt agaagttcca gtgagccaga tcgacaaagt attagaaaat
      781 aaaatgatgt ttgacggttc ttcgattgaa ggatttgttc gaattgaaga aagcgatatg
      841 tatttgtatc cagatctctc cacttggatg atcttcccgt gggaaagtgc tcatggaaaa
      901 gttgctcgtt taatttgtga tatctataat ccggatggga caccatttgc cggcgatcct
      961 cgcgggaatt tgaaacgagc attgaaagat atgcgcgacc ttggttttac ctctttcaat
     1021 ttaggacctg aaccagaatt cttcttattt aaattagacg aagacggtgg gatcacaacg
     1081 accttgaatg ataaaggtgg ttattttgat tttgcaccaa cagatttagg tgaaaattgc
     1141 cgccgtgaca tcgtgttgga attagaaagt ctaggctttg aagtggaagc ttcacatcat
     1201 gaagtagcgc caggacagca tgaaattgac tttaaatatg cagatgtggt agatgcttgt
     1261 gataatatcc aaaccttcaa gctcgttgtc aaaacgattg cacgcaagca tggacttcac
     1321 gcgactttca tgccgaaacc gttatttggg atcaatggtt ctggtatgca ttgcaatatg
     1381 tctttgttca atgatgaagg aaatgttttc tatgataaag acggtgaatt ggaactaagt
     1441 gaaacagctt atcatttctt aggtggtttg ctgaaacatg ctcgtgccta cacagccgtt
     1501 tgcaatccaa cagtcaattc ttataaacgt ctcgttccag gttatgaagc gcctgtttat
     1561 gtcgcatgga gtggccgtaa tcgttcgcca ttagtacgtg taccagaatc tcgcggttta
     1621 tccacacgtt tggaattgcg gtcagttgat ccatctgcaa acccatactt ggcaatggca
     1681 gtattgttgc aagcaggact agacggtatc cgtaatcaga tcacaccgcc agcagctgtt
     1741 gaccgtaaca tttatgtaat ggatgaggaa gaaagagaag ctgcacatat ccaggactta
     1801 ccttcaacta tccataatgc tatcaaagaa ttgcgtaagg ataatgtaat gattgacgca
     1861 ttaggacaac atatcttctc aaattttgtt gaagcaaaac gtctcgaatg ggcggcattc
     1921 agacaaactg tttccgagtg ggaaagagaa caatatctag aactttatta aaacaggaat
     1981 accgtgaatt agaggctgtg aaatcagcgt tttgacctga gtattagaga aactaacgga
     2041 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
     2101 accgtttagt ggagaggttg tgaaaaagct gccctgcaac aagtaataag aatagccaag
     2161 caaaaacagc ttttcttgtt tttgaattgg ctgaacttat taccgcagat gcaagctttt
     2221 gaacaccgtt taccaagagg ttttgaaaaa gacgtttagt ttcgagtatt aagaaaaatt
     2281 ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag aaactggctt
     2341 ttgaataccg tttatccaga ggttgtgagc ctaccattta actccgaatc acaagaaata
     2401 atttcaaaaa cagcttctca tgtttgatgg atattgtgac ttgtgaccga ggagttggca
     2461 ggagaacacc gtttatttag aaataaggag gcacgacttt tgtcgcatct ccttattttt
     2521 tacttatgtg attcgttacc ttcaatgaat ccctacaagg cacttttgtt gaaaaaaagg
     2581 tcatctttga aaaaattagt ggaattgttt tgtttttgat attatttaat agaaaagata
     2641 tgccaaaaat ccgagaactg tggactacgt tttttacgta aaggaggtaa ataagggtga
     2701 aagaaaaaga ttgtttctac gataagctat ataaactgtt gctgcaagta ggaaaaagta
     2761 gttctttgtt aggtgggata ttatctatca taatgtttgt tttatttttt tatctattat
     2821 ttcacttaaa atttttcagt aacgattttc tgatttgctt aggtttaacg ttagctatgt
     2881 ttatatcaag cttattatgt gggttaaatt ttttttgcac tcataaagga cttaagaaat
     2941 aatttctcaa aggatatcga aaaagaaaaa attgtaagcg gatagtattc atttgagtac
     3001 ttgactttcc cttagtcagc ttgtattata caccttgggc acccttttag agggtcagaa
     3061 gtttacagaa atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aactttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aattttttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggcatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagaacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgatt tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa
    34621 aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa
    34681 acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga
    34741 ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg
    34801 gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct
    34861 gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga
    34921 gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc
    34981 gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg
    35041 cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca
    35101 atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt
    35161 tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac
    35221 catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa
    35281 caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg
    35341 taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag
    35401 gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg
    35461 ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat
    35521 tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg
    35581 ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt
    35641 ctactccatc atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc
    35701 cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc
    35761 tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg
    35821 tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga
    35881 ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa
    35941 aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca
    36001 atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag
    36061 gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc
    36121 aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag
    36181 acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc
    36241 tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat
    36301 ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt
    36361 atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg
    36421 aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt
    36481 tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt
    36541 accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta
    36601 ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg
    36661 ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt
    36721 agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc
    36781 gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga
    36841 aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt
    36901 ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct
    36961 ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt
    37021 tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt
    37081 agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta
    37141 cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac
    37201 aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa
    37261 tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt
    37321 atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat
    37381 gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg
    37441 gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat
    37501 ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc
    37561 cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga
    37621 taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg
    37681 aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga
    37741 aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa
    37801 ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga
    37861 gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta
    37921 tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg
    37981 tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct
    38041 cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg
    38101 aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc
    38161 cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg
    38221 ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca
    38281 agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt
    38341 actttaatac gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag
    38401 taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga
    38461 atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat
    38521 ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca
    38581 atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac
    38641 aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga
    38701 ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag
    38761 agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc
    38821 agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg
    38881 ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca
    38941 aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc
    39001 aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg
    39061 ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc
    39121 atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat
    39181 taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga
    39241 ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca
    39301 atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga
    39361 tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga
    39421 atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa
    39481 acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt
    39541 tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg
    39601 ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca
    39661 cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta
    39721 tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg
    39781 gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag
    39841 taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt
    39901 attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca
    39961 agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc
    40021 ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga
    40081 ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa
    40141 aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac
    40201 agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc
    40261 gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga
    40321 agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga
    40381 aacagttgaa aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat
    40441 cggaaaaaag cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc
    40501 ttatctataa tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg
    40561 gaagaaagca tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca
    40621 ttcaactatg cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt
    40681 aaccttttat tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg
    40741 atcaattcag tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc
    40801 agcggagtga gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc
    40861 atgccagaag attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt
    40921 tcagcactca tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc
    40981 gactatcctg atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat
    41041 agaattggta cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca
    41101 atcgctttgt tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa
    41161 ttttatgaac aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta
    41221 agcagtttat tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt
    41281 acgatgaaat taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca
    41341 acaggttatt tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg
    41401 ctgatcaaat tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat
    41461 tatcatcatg atcatattgc tgaccttggt gtattacagt attactggca acttcatcct
    41521 aagagaaatc cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt
    41581 gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta
    41641 aaaattggtc cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc
    41701 atgcgtatcg tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat
    41761 ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt
    41821 tttgaaggaa atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg
    41881 aaatcagcta acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa
    41941 caattgaaaa atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca
    42001 gttgacaaag tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca
    42061 cgacccaaga atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga
    42121 acctattttg ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac
    42181 gcttgaagaa atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt
    42241 aagtggaggc ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc
    42301 tgatgatggg gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact
    42361 tcatgaattg ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg
    42421 catgaaattt tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac
    42481 aattatgttc gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa
    42541 aatcgaatca aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct
    42601 tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat
    42661 tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa
    42721 aaaaatcaat cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc
    42781 tccagatttc aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt
    42841 gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg
    42901 agatatctct gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc
    42961 aaaaatagtc agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt
    43021 gcttacctta ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat
    43081 tttttataca ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt
    43141 atctttttcc tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa
    43201 gtcaaaacgt tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc
    43261 gtaatgtgca ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa
    43321 ataaattttt cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa
    43381 agcgtggatc aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc
    43441 tgcttggaag gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat
    43501 aagatttgtc acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc
    43561 taatatcttt tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag
    43621 attctccatc tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg
    43681 aagctgctca taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct
    43741 ccgatctttg tcggtattta agatgtgatc gattagattg tagctgataa taacgattag
    43801 aatgataata tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt
    43861 ttggatgtaa atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac
    43921 ataaaaacgg ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct
    43981 tataacacta gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt
    44041 tgcataaagc agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca
    44101 ttttttatag taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat
    44161 aataaataaa tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat
    44221 atcttctgat acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga
    44281 gtattcaaga cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc
    44341 gataaaaaca gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact
    44401 aatcaatggt ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata
    44461 aggagatgag actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac
    44521 agccaatgag agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt
    44581 ttcgcctaga tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc
    44641 caacggtttt tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat
    44701 cgtacgagtg tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca
    44761 cgaatctttt atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa
    44821 ataagcaatt cctgttaaaa ataacaatga taatataatg attatgctcc actttatacg
    44881 taaataaaaa gattcttttt tattatatat gttattaaaa aaagacataa aggtattcct
    44941 ttctaaaact tgtaatgtta gggtaaacgt tttttaaact aacgtcaatt atttataata
    45001 aaaaatgaga taaagatgag gaaatagaat tagaaacata taattattaa agggaaacat
    45061 ttttacagct ataatttgaa aaaaatttat cgttattata tgattaagaa ggattgaata
    45121 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    45181 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    45241 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    45301 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    45361 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    45421 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    45481 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    45541 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    45601 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    45661 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    45721 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    45781 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    45841 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    45901 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    45961 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    46021 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    46081 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    46141 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    46201 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    46261 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    46321 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    46381 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    46441 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    46501 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    46561 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    46621 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    46681 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    46741 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    46801 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    46861 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    46921 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    46981 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    47041 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    47101 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    47161 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    47221 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    47281 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    47341 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    47401 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    47461 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    47521 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    47581 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    47641 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    47701 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    47761 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    47821 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    47881 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    47941 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    48001 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    48061 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    48121 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    48181 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    48241 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    48301 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    48361 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    48421 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    48481 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
    48541 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    48601 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    48661 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    48721 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    48781 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    48841 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    48901 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    48961 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    49021 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    49081 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    49141 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    49201 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    49261 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    49321 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    49381 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    49441 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    49501 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    49561 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    49621 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    49681 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    49741 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    49801 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    49861 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    49921 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    49981 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    50041 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    50101 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    50161 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    50221 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    50281 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    50341 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    50401 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    50461 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    50521 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    50581 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    50641 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    50701 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    50761 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    50821 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    50881 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    50941 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    51001 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    51061 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    51121 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    51181 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    51241 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    51301 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    51361 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    51421 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    51481 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    51541 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    51601 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    51661 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    51721 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    51781 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    51841 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    51901 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    51961 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    52021 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    52081 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    52141 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    52201 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    52261 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    52321 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    52381 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    52441 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    52501 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    52561 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    52621 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    52681 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    52741 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    52801 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    52861 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    52921 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    52981 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    53041 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    53101 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    53161 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    53221 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    53281 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    53341 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    53401 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    53461 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    53521 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    53581 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    53641 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    53701 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    53761 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    53821 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    53881 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    53941 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    54001 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    54061 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    54121 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    54181 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    54241 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    54301 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    54361 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    54421 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    54481 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    54541 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    54601 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    54661 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    54721 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    54781 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    54841 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    54901 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    54961 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    55021 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    55081 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    55141 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    55201 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    55261 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    55321 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    55381 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    55441 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    55501 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    55561 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    55621 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    55681 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    55741 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    55801 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    55861 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    55921 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    55981 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    56041 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    56101 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    56161 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    56221 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    56281 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    56341 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    56401 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    56461 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    56521 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    56581 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    56641 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    56701 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    56761 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    56821 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    56881 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    56941 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    57001 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    57061 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    57121 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    57181 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    57241 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    57301 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    57361 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    57421 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    57481 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    57541 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    57601 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    57661 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    57721 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    57781 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    57841 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    57901 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    57961 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    58021 tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt accatgcgcg
    58081 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    58141 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    58201 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    58261 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    58321 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    58381 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    58441 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    58501 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    58561 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    58621 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    58681 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    58741 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    58801 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    58861 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    58921 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    58981 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    59041 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    59101 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    59161 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    59221 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    59281 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    59341 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    59401 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    59461 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    59521 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    59581 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    59641 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    59701 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    59761 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    59821 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    59881 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    59941 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    60001 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    60061 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    60121 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    60181 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    60241 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    60301 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    60361 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    60421 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    60481 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    60541 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    60601 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    60661 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    60721 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    60781 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    60841 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    60901 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    60961 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    61021 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    61081 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    61141 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    61201 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    61261 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    61321 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    61381 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    61441 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    61501 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    61561 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    61621 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    61681 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    61741 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    61801 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    61861 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    61921 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    61981 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    62041 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    62101 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    62161 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    62221 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    62281 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    62341 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    62401 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    62461 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    62521 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    62581 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    62641 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    62701 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    62761 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    62821 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    62881 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    62941 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    63001 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    63061 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    63121 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    63181 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    63241 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    63301 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    63361 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    63421 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    63481 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    63541 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    63601 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    63661 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    63721 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    63781 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    63841 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    63901 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    63961 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    64021 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    64081 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    64141 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    64201 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    64261 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    64321 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    64381 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    64441 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    64501 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    64561 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    64621 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    64681 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    64741 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    64801 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    64861 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    64921 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    64981 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    65041 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    65101 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    65161 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    65221 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    65281 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    65341 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    65401 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    65461 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    65521 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    65581 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    65641 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    65701 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    65761 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    65821 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    65881 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    65941 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    66001 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    66061 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    66121 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    66181 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    66241 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    66301 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    66361 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    66421 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    66481 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    66541 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    66601 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    66661 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    66721 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    66781 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    66841 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    66901 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    66961 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    67021 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    67081 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    67141 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    67201 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    67261 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    67321 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    67381 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    67441 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    67501 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    67561 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    67621 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    67681 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    67741 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    67801 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    67861 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    67921 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    67981 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    68041 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    68101 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    68161 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    68221 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    68281 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    68341 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    68401 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    68461 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    68521 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    68581 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    68641 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    68701 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    68761 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    68821 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    68881 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    68941 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    69001 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    69061 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    69121 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    69181 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    69241 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    69301 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    69361 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    69421 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    69481 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    69541 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    69601 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    69661 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    69721 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    69781 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    69841 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    69901 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    69961 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    70021 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    70081 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    70141 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    70201 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    70261 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    70321 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    70381 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    70441 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    70501 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    70561 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    70621 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    70681 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    70741 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    70801 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    70861 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    70921 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    70981 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    71041 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    71101 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    71161 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    71221 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    71281 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    71341 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    71401 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    71461 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    71521 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    71581 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    71641 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    71701 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    71761 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    71821 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    71881 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    71941 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    72001 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    72061 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    72121 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    72181 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    72241 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    72301 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    72361 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    72421 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    72481 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    72541 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    72601 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    72661 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    72721 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    72781 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    72841 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    72901 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    72961 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    73021 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    73081 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    73141 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    73201 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    73261 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    73321 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    73381 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    73441 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    73501 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    73561 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    73621 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    73681 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    73741 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    73801 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    73861 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    73921 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    73981 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    74041 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    74101 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    74161 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    74221 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    74281 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    74341 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    74401 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    74461 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    74521 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    74581 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    74641 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    74701 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    74761 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    74821 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    74881 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    74941 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    75001 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    75061 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    75121 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    75181 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    75241 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    75301 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    75361 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    75421 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    75481 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    75541 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    75601 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    75661 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    75721 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    75781 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    75841 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    75901 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    75961 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    76021 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    76081 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    76141 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    76201 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    76261 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    76321 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    76381 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    76441 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    76501 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    76561 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    76621 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    76681 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    76741 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    76801 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    76861 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    76921 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    76981 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    77041 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    77101 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    77161 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    77221 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    77281 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    77341 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    77401 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    77461 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    77521 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    77581 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    77641 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    77701 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    77761 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    77821 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    77881 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    77941 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    78001 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    78061 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    78121 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    78181 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    78241 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    78301 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    78361 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    78421 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    78481 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    78541 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    78601 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    78661 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    78721 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    78781 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    78841 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    78901 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    78961 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    79021 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    79081 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    79141 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    79201 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    79261 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    79321 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    79381 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    79441 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    79501 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    79561 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    79621 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    79681 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    79741 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    79801 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    79861 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    79921 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    79981 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    80041 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    80101 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    80161 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    80221 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    80281 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    80341 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    80401 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    80461 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    80521 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    80581 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    80641 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    80701 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    80761 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    80821 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    80881 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    80941 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    81001 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    81061 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    81121 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    81181 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    81241 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    81301 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    81361 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    81421 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    81481 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    81541 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    81601 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    81661 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    81721 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    81781 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    81841 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    81901 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    81961 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    82021 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    82081 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    82141 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    82201 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    82261 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    82321 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    82381 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    82441 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    82501 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    82561 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    82621 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    82681 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    82741 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    82801 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    82861 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    82921 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    82981 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    83041 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    83101 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    83161 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    83221 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    83281 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    83341 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    83401 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    83461 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    83521 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    83581 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    83641 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    83701 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    83761 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    83821 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    83881 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    83941 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    84001 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    84061 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    84121 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    84181 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    84241 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    84301 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    84361 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    84421 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    84481 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    84541 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    84601 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    84661 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    84721 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    84781 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    84841 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    84901 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    84961 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    85021 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    85081 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    85141 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    85201 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    85261 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    85321 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    85381 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    85441 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    85501 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    85561 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    85621 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    85681 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    85741 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    85801 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    85861 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    85921 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    85981 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    86041 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    86101 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    86161 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    86221 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    86281 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    86341 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    86401 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    86461 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    86521 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    86581 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    86641 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    86701 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    86761 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    86821 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    86881 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    86941 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    87001 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    87061 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    87121 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    87181 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    87241 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    87301 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    87361 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    87421 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    87481 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    87541 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    87601 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    87661 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    87721 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    87781 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    87841 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    87901 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    87961 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    88021 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    88081 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    88141 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    88201 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    88261 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    88321 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    88381 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    88441 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    88501 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    88561 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    88621 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    88681 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    88741 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    88801 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    88861 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    88921 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    88981 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    89041 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    89101 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    89161 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    89221 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    89281 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    89341 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    89401 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    89461 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    89521 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    89581 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    89641 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    89701 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    89761 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    89821 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    89881 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    89941 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    90001 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    90061 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    90121 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    90181 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    90241 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    90301 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    90361 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    90421 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    90481 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    90541 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    90601 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    90661 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    90721 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    90781 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    90841 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    90901 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    90961 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    91021 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    91081 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    91141 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    91201 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    91261 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    91321 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    91381 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    91441 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    91501 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    91561 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    91621 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    91681 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    91741 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    91801 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    91861 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    91921 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    91981 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    92041 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    92101 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    92161 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    92221 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    92281 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    92341 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    92401 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    92461 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    92521 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    92581 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    92641 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    92701 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    92761 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    92821 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    92881 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    92941 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    93001 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    93061 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    93121 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    93181 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    93241 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    93301 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    93361 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    93421 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    93481 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    93541 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    93601 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
    93661 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    93721 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    93781 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    93841 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    93901 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    93961 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    94021 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    94081 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    94141 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    94201 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    94261 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    94321 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    94381 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    94441 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    94501 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    94561 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    94621 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    94681 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    94741 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    94801 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    94861 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    94921 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    94981 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    95041 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    95101 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    95161 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    95221 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    95281 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    95341 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    95401 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    95461 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    95521 gacgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    95581 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    95641 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    95701 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    95761 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    95821 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    95881 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    95941 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    96001 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    96061 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    96121 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    96181 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    96241 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    96301 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    96361 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    96421 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    96481 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    96541 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    96601 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    96661 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    96721 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    96781 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    96841 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    96901 tgcgtgatgc ttttatcggc acactgcccg caacaatggc gggatcagtc gctgtaatga
    96961 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    97021 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    97081 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    97141 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    97201 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    97261 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    97321 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    97381 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    97441 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    97501 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    97561 ggcaactgat cattgatttg gtgcaaaaat atattgcaga gccattccta ggattatctc
    97621 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    97681 ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    97741 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    97801 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    97861 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    97921 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    97981 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    98041 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    98101 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    98161 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    98221 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    98281 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    98341 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    98401 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    98461 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    98521 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    98581 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    98641 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    98701 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    98761 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    98821 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    98881 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    98941 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    99001 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    99061 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    99121 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    99181 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    99241 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    99301 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    99361 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    99421 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    99481 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    99541 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    99601 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    99661 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    99721 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    99781 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    99841 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    99901 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    99961 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
   100021 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
   100081 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
   100141 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
   100201 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
   100261 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
   100321 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
   100381 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
   100441 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
   100501 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
   100561 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
   100621 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
   100681 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
   100741 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
   100801 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
   100861 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
   100921 cctaagcgat aggtcgctgg ttcaattc
//
LOCUS       DACDKJ010000003        94266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDKJ010000003 DACDKJ010000000
VERSION     DACDKJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.003"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV328_000211"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV328_000211"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8936383.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV328_000212"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV328_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8936384.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV328_000213"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV328_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8936385.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV328_000214"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV328_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8936386.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV328_000215"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV328_000215"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8936387.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV328_000216"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV328_000216"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8936388.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IV328_000217"
     CDS             complement(8053..8523)
                     /locus_tag="IV328_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8936389.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IV328_000218"
     CDS             complement(8569..8994)
                     /locus_tag="IV328_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8936390.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IV328_000219"
     CDS             complement(9061..9330)
                     /locus_tag="IV328_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8936391.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IV328_000220"
     CDS             complement(9341..10936)
                     /locus_tag="IV328_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8936392.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV328_000221"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV328_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8936393.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IV328_000222"
     CDS             complement(14489..15049)
                     /locus_tag="IV328_000222"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8936394.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IV328_000223"
     CDS             15402..16376
                     /locus_tag="IV328_000223"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8936395.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IV328_000224"
     CDS             16546..17946
                     /locus_tag="IV328_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8936396.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IV328_000225"
     CDS             complement(18024..18746)
                     /locus_tag="IV328_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8936397.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IV328_000226"
     CDS             complement(18825..19331)
                     /locus_tag="IV328_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8936398.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IV328_000227"
     CDS             19454..21253
                     /locus_tag="IV328_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8936399.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IV328_000228"
     CDS             complement(21321..22895)
                     /locus_tag="IV328_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936400.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV328_000229"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV328_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8936401.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV328_000230"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV328_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8936402.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IV328_000231"
     CDS             complement(24672..25310)
                     /locus_tag="IV328_000231"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8936403.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IV328_000232"
     CDS             complement(25311..25961)
                     /locus_tag="IV328_000232"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8936404.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV328_000233"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV328_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8936405.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV328_000234"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV328_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8936406.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IV328_000235"
     CDS             complement(29104..29844)
                     /locus_tag="IV328_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8936407.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV328_000236"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV328_000236"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8936408.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IV328_000237"
     CDS             complement(31215..32153)
                     /locus_tag="IV328_000237"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8936409.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV328_000238"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV328_000238"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8936410.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV328_000239"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV328_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8936411.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IV328_000240"
     CDS             complement(35313..35618)
                     /locus_tag="IV328_000240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8936412.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV328_000241"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV328_000241"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8936413.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IV328_000242"
     CDS             complement(36724..37008)
                     /locus_tag="IV328_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936414.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IV328_000243"
     CDS             complement(37026..37916)
                     /locus_tag="IV328_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8936415.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IV328_000244"
     CDS             complement(37936..38154)
                     /locus_tag="IV328_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936416.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IV328_000245"
     CDS             complement(38124..39824)
                     /locus_tag="IV328_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8936417.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IV328_000246"
     CDS             40091..40864
                     /locus_tag="IV328_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936418.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IV328_000247"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IV328_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8936419.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IV328_000248"
     CDS             complement(41688..42659)
                     /locus_tag="IV328_000248"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8936420.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IV328_000249"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IV328_000249"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8936421.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IV328_000250"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IV328_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8936422.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IV328_000251"
     CDS             complement(45614..46438)
                     /locus_tag="IV328_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8936423.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IV328_000252"
     CDS             complement(46411..47091)
                     /locus_tag="IV328_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8936424.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IV328_000253"
     CDS             complement(47114..48052)
                     /locus_tag="IV328_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8936425.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /locus_tag="IV328_000254"
     CDS             complement(48297..49148)
                     /locus_tag="IV328_000254"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8936426.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IV328_000255"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IV328_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8936427.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="IV328_000256"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="IV328_000256"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8936428.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IV328_000257"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IV328_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8936429.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IV328_000258"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IV328_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8936430.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="IV328_000259"
     CDS             complement(56026..57267)
                     /locus_tag="IV328_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8936431.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IV328_000260"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IV328_000260"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8936432.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59178)
                     /locus_tag="IV328_000261"
     CDS             complement(58294..59178)
                     /locus_tag="IV328_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8936433.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="IV328_000262"
     CDS             complement(59956..61242)
                     /locus_tag="IV328_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8936434.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="IV328_000263"
     CDS             complement(61235..62503)
                     /locus_tag="IV328_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8936435.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="IV328_000264"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="IV328_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8936436.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="IV328_000265"
     CDS             complement(63276..63722)
                     /locus_tag="IV328_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8936437.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63815..64060)
                     /locus_tag="IV328_000266"
     CDS             complement(63815..64060)
                     /locus_tag="IV328_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936438.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64176..64754)
                     /locus_tag="IV328_000267"
     CDS             complement(64176..64754)
                     /locus_tag="IV328_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8936439.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64831..65361)
                     /locus_tag="IV328_000268"
     CDS             complement(64831..65361)
                     /locus_tag="IV328_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8936440.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65365..66225)
                     /gene="rlmB"
                     /locus_tag="IV328_000269"
     CDS             complement(65365..66225)
                     /gene="rlmB"
                     /locus_tag="IV328_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8936441.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66218..66613)
                     /locus_tag="IV328_000270"
     CDS             complement(66218..66613)
                     /locus_tag="IV328_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8936442.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66610..68022)
                     /locus_tag="IV328_000271"
     CDS             complement(66610..68022)
                     /locus_tag="IV328_000271"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8936443.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68019..68555)
                     /gene="cysE"
                     /locus_tag="IV328_000272"
     CDS             complement(68019..68555)
                     /gene="cysE"
                     /locus_tag="IV328_000272"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8936444.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(68877..70337)
                     /locus_tag="IV328_000273"
     CDS             complement(68877..70337)
                     /locus_tag="IV328_000273"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8936445.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70507..71664)
                     /locus_tag="IV328_000274"
     CDS             complement(70507..71664)
                     /locus_tag="IV328_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8936446.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71698..73068)
                     /gene="radA"
                     /locus_tag="IV328_000275"
     CDS             complement(71698..73068)
                     /gene="radA"
                     /locus_tag="IV328_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8936447.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73131..73658)
                     /locus_tag="IV328_000276"
     CDS             complement(73131..73658)
                     /locus_tag="IV328_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8936448.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73888..74694
                     /gene="proB"
                     /locus_tag="IV328_000277"
     CDS             73888..74694
                     /gene="proB"
                     /locus_tag="IV328_000277"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8936449.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74691..75938
                     /locus_tag="IV328_000278"
     CDS             74691..75938
                     /locus_tag="IV328_000278"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8936450.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76014..76358
                     /locus_tag="IV328_000279"
     CDS             76014..76358
                     /locus_tag="IV328_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936451.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76475..76801)
                     /locus_tag="IV328_000280"
     CDS             complement(76475..76801)
                     /locus_tag="IV328_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936452.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77416..78618)
                     /locus_tag="IV328_000281"
     CDS             complement(77416..78618)
                     /locus_tag="IV328_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8936453.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78735..79250
                     /locus_tag="IV328_000282"
     CDS             78735..79250
                     /locus_tag="IV328_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8936454.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79449..79895)
                     /locus_tag="IV328_000283"
     CDS             complement(79449..79895)
                     /locus_tag="IV328_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8936455.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79942..81420)
                     /gene="msr"
                     /locus_tag="IV328_000284"
     CDS             complement(79942..81420)
                     /gene="msr"
                     /locus_tag="IV328_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8936456.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81774..82403)
                     /locus_tag="IV328_000285"
     CDS             complement(81774..82403)
                     /locus_tag="IV328_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8936457.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82387..82851)
                     /locus_tag="IV328_000286"
     CDS             complement(82387..82851)
                     /locus_tag="IV328_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8936458.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83338..84606)
                     /locus_tag="IV328_000287"
     CDS             complement(83338..84606)
                     /locus_tag="IV328_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8936459.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84837..85574)
                     /locus_tag="IV328_000288"
     CDS             complement(84837..85574)
                     /locus_tag="IV328_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8936460.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85641..86210)
                     /locus_tag="IV328_000289"
     CDS             complement(85641..86210)
                     /locus_tag="IV328_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8936461.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86338..87210
                     /locus_tag="IV328_000290"
     CDS             86338..87210
                     /locus_tag="IV328_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8936462.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87552..88424
                     /locus_tag="IV328_000291"
     CDS             87552..88424
                     /locus_tag="IV328_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8936463.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88421..90379
                     /locus_tag="IV328_000292"
     CDS             88421..90379
                     /locus_tag="IV328_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8936464.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90711..91562)
                     /locus_tag="IV328_000293"
     CDS             complement(90711..91562)
                     /locus_tag="IV328_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8936465.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91685..92152
                     /locus_tag="IV328_000294"
     CDS             91685..92152
                     /locus_tag="IV328_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8936466.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92415..92897)
                     /locus_tag="IV328_000295"
     CDS             complement(92415..92897)
                     /locus_tag="IV328_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8936467.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92997..93860)
                     /locus_tag="IV328_000296"
     CDS             complement(92997..93860)
                     /locus_tag="IV328_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8936468.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        26339 a        20305 c        16934 g        30688 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat tgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tctcaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    63781 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
    63841 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
    63901 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
    63961 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
    64021 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
    64081 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaag aaatgcaata
    64141 aaaataaata agaagctcat taagattttt ctaaattatt cctcattata aaatttcaag
    64201 tgttttttta ttttgagttt tactctcccg taagcgctca ttacttgctt gtgatccatt
    64261 gctaaacatt cagccatgtc tacaaaatca acgccttgca tataataatg aaaaatttta
    64321 gcttctattt ttgaaaactg gatttcgcaa ttttcgattg cttcttttaa taaaagatgc
    64381 tgaattgcta aaggttcttg caatgtgatc tctgctaaaa agtctggtcc ttctgtctct
    64441 attttttggt cgattgagac ttcatctttt tgatcacgac gcttcagcgc gttctgcttc
    64501 ctcaacagag aatagatttg attgcttata acgcttttat aataacttcc aaagctaaca
    64561 cctttgcttt tttcgtaagt tctcaatgcc tgatataaag cgatccttgc ttcttgttcc
    64621 caatcctcag aaacataatt tcttaaataa taggtgtttt tcaaacgtag taaaagtgga
    64681 aaatattttt gatacagggc agcaaatgct tcttcgtcac cattacgtgc agcgcgcaat
    64741 aactcctcag acataaaata attcctccat aaattcgttt atggaggaat cttatcatca
    64801 atgattcatc gaactgatta gaacgatatg ttatttattt tttctcatta aatcgtccaa
    64861 tttttgggac aattttttta actgctcttc attccatgga gaattacgtc ggaaatcaat
    64921 aaaatgcata tctttatttt cttgggctat tgttttttcc gtttttttta tcgtcttata
    64981 taattcataa gcagaagttc gtaatgctcc ttgggaaaag atcatccact gttctgctaa
    65041 atcgcttgtc gccactgtca cttgtgtcaa acggtcattc agctgaccag cgattctctc
    65101 gatataactg tcagccgttt cgtcttcttt cgtaaaaaca acatccaact ggtatttctt
    65161 ataattttgt tggatgcccg gaactagttg ggcatcgaag acaacgatga cttccattcc
    65221 ttcatatttc gcataatttg ataaacgatg taaaagagct tctcttgcat ccgccatttt
    65281 gtcctgcttt ttaagctgga caagttccgg ccacgaacca atcatgttgt agccgtctac
    65341 aatcaacagc tgttttttca tggtctactc cccctgacgt tgggaaaaag cctgatacat
    65401 aagcaaacca gcagcaacac tggcatttaa gctctggacg tgcccggtca tcgggatcgt
    65461 cagcaattca tccatttctt ttttcaatcc tggactcatt cctttgcctt cattaccaat
    65521 gactaaagca attgatcctt tggcattcca tttttggaag tttgtgccgg tcatatctgt
    65581 gccaaatacc caaaatcctg tttctttgag ctgttgcaca gcttgtacca aattcgtcac
    65641 gcgtgcaaca gggacatgtt cgactgcccc agtggaagtc tttgttacga taggtgtgat
    65701 tccaactgct cggtgttttg gtatgatcac accatcgacg ccacttgcgt ctgctgtccg
    65761 taaaatcgac ccaaaattgt gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc
    65821 ttcttttttc tcttttgttc gttcaatcag ttggtcaagg gataaatatt cataaggtgt
    65881 aatcgcaaga acgactcctt gatgcacgcc gtgatcgctc aaagtttcta atttttgttt
    65941 aggaacccat ttgactggca cagcttgttc acgtgcgatt tctttcagtt cttctacttt
    66001 ttctcctcgt gaatcttctt gaagaaagag tttattccct cgcccttgtt gtagcgcttc
    66061 tactgcggca tggtgcccaa agacgaaatt atcttctaat gcttcttcat tttgatcaga
    66121 aactgactga tttcgtccag tttgcttttt atttgaatat ctattttgtt ttttgtcttc
    66181 aaatccacgt ttcgattgat gtttgtttgt tctttcttta cccatttttt tcaccaactt
    66241 tctgaataca ccagcggatc aattcttcca tccgctcggt ttgttttgtc aaatgcagat
    66301 agcccatgag cgcttcgaac cctgttgcga cccgatacgt cgtaacatca gcattttttg
    66361 cgctcgtatg gcttttagca tttcttcctc tgcgataata aagttcttct tcttctgtca
    66421 aaagatgttc ttccagcatt tgctgaatca aaaaggcttg tgcattcgca gaaacataat
    66481 tcgttgccgc acgatgcagc ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac
    66541 gaacatagat ttcgtaaatc gcatctccaa cataagccaa ggctaaaccg ttcaattgtg
    66601 tgtaatctct catgcttctc ttctccatcg ggttccttgc ggtgtatctt ctaataagat
    66661 ccctttttct ttcaacaaat cacgaatttc gtcgcttcgg acaaaatcac gattgcttct
    66721 tgcttgattt cgttcttcga tcaattgttc aatctcttca tctaataact cttctgctgt
    66781 aaataaaata ccgaagatcg aaagccattg cgagaataaa gcttctcctt gtgccaagac
    66841 agcggcagaa actttcggct ggtctagata agtgttcagc cacttagcaa gctcatagac
    66901 cactgtgatc ccatttgctg cattaaaatc atcgtccatt tcttcgatga atcgctgttc
    66961 taaattttct aacaaagcga ttttatccgc atcatctgcc aaattctctg aggcagtttc
    67021 ttggcggaat ttcaaattgt caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc
    67081 tttcatcgtc gtttcactgt agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa
    67141 acgcaagact tgtggatcaa cgtttttgat catttcatgt acagtgatga aattccctag
    67201 agatttactc attttttcat cgtcttcgcc aatcgttaca tagccattgt gcatccagta
    67261 attagcgaat tttttccctg ttttcgcttc actttgtgcg atttcatttt catgatgagg
    67321 aaattctaaa tcttgcccgc caccatggat atcgatcgtt tctcctaaat gttttgttgc
    67381 cataacggaa cattcaatat gccaaccggg acgtcctttg ccccatggtg aatcccaaga
    67441 aatctcgcca ggttttgctt gtttccataa agcgaaatct aatgggtctt ctttttgctg
    67501 ctgttcttcc cctgtacgct gacttgctcc gacttccagt tcatcgatgg attggtcact
    67561 taatttccca taattttcaa atttacgggt acggtaatac acatcacctg aagattcata
    67621 agcatagcct ttttcaacca acacttgaat aaatgctaaa atatctggca tatgatccat
    67681 gactcttgga tgcagtgtag caggttcgac attcaacgct ttcgtatctt cttcaaatgc
    67741 tcggatgaaa cgatcagcaa cttctggtgc tgtaatacct aattcgtttg ctgtacggat
    67801 gattttatcg tctacatccg tgaaattcga tacgaaatcg actttatagc ctcgatattc
    67861 aaaatagcgt cgaattgtat cgaaagcaat ggtgctacga gcatttccga tatggatata
    67921 attatacaca gtcggtccgc agacatacat accgacatgt cccttttgta tagggatgaa
    67981 ttcttctttt tttctggtca atgtattgta gatttttatc attctgatct cctcactttt
    68041 tctcctttga ttcgaacgac ttttgctggt actcctaccg ctgtagcgtc gtctggaaca
    68101 tctttcaaca caacggcacc tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct
    68161 aatatctgcg cattcgcaga gagcatggct ccttgtttaa cagttgggtg tcttttaccc
    68221 gtttcttttc ctgtgccgcc taatgttacg ccatggaata aaatcacatc gtcctcgatt
    68281 tctgcggttt cgccaatgac gattcccatt ccatggtcga tgaacacgcc agttccaatc
    68341 gttgctcctg gatggatctc aacaccagtt aggaaacgcc agaattgagc attgatcttc
    68401 gccaataaaa agagccggtg ctgatataaa aaatgagaga aacgatgcca aaacaaggca
    68461 tgaaaaccag ggtatgtcaa aacgatctca gctgtcgaac gtgcagccgg atcgtttctc
    68521 tttgctgcat caatcgctcg ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat
    68581 tttatgtagg aaatcatctt cttataaaga aaagaagcgc cttttaaata agtttcctta
    68641 tcaaaagacg ctttccgcgt ggttccactt ttcttcataa tcaatgatta tctctgacag
    68701 ctaacgcctg ttatacgttc ttggctactt tgtttcctat aaaacagttt ttcctgtttt
    68761 ctgtgtttcg ccaagaccac tcccagaggc atttcggcaa ccattcattg ataccttgca
    68821 ccacccggta tctctcttgt aatgatcagc tgcttacttt tttctgttca tcgtgtttag
    68881 ttcagtactt gatttaaatg tgctaatgat ttttcacgac caagcaattc gatcgtttca
    68941 ggcaattctg gaccatgcat ttgtcctgat acagctacac ggattggcat gaaaagattt
    69001 tttcctttta cgccagtttc tttttgaaca gctttgatgg cagctttgac agttggagca
    69061 tctactactt ccatttgttc tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt
    69121 tcaccagcaa gtacttcttt tgctgcttca ttcaatacag gatgttcatt gaaaaatagc
    69181 gtagacagat caacgatttc tgcagcatag ctcatttgag gttgatacag gctaacgatc
    69241 ttcttcaccc attcgattgt cttttcatct gggttttctg ctacacgtcc atcagcgatt
    69301 agataaggca gacacatgtc agtaaatgca tctagatcca ttgctttgat gtaatggttg
    69361 tttacccatt gcagtttttt cgcatcgaaa gctgcgggtg atttacttag gcgttttgga
    69421 tcgaacattt tgatcaattc ttggtgttcg aagatttcgt cttcgcccac aggtgaccag
    69481 cccaataatg caataaagtt gaacattgca tctggtagat agcctaattc gcgatattgt
    69541 tcgataaatt gcaagattga ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg
    69601 atgatcaatg tcatatgtcc gaaggttggt ggtgtccagc caaatgcttc atagatcatc
    69661 agttgttttg gtgtattggc aatatggtca tctccacgta aaacgtgagt gattttcatc
    69721 aaatgatcat cgaccgcaac agcaaaatta taagttggca taccatcgcg tttttggatg
    69781 acaaagtcgc caccgatatt atcggattcg aacgaaatag cgcctttcac catatctgta
    69841 aatgcgtatt ctgtattgcg aggcacacgg aaacgtacaa cagattctag accttgtgct
    69901 tctttttctg cttgttcttc aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt
    69961 tctcctcttg cgcgttgtgc ttcccgttct gcttctaatt cttcttctgt gcaatagcat
    70021 ttgtatgctc gattgctagc aagcagctga tcaatcagcg gttgatagat ttctttacgt
    70081 tcagattgac ggtaagggcc gtattctcca ggattttcag gagattcatc ccaattcatt
    70141 cccaaccaag caaggttttc caactggctt ttttcgccat cttcgatatt acgtttttgg
    70201 tcagtatcct caatacgaat aataaagtct ccatcattgt ggcgggcaaa caaataattg
    70261 aataatgcag ttcttgcatt tccaatatgc aggtgcccag ttggacttgg tgcatagcgt
    70321 acgcgtactt tcgtcatttt cttcctcttc tttcaaacta gactttgact aacagcaaaa
    70381 attccgtcag ttacggcttc tcaaatatca tttatttaaa gccactcagc acaaatttta
    70441 ccttttctaa ggtaaatagt aaagccaact ggttgaattt ccttaccagt tggctgaatt
    70501 tcttttttat tttgattgtt tagtcttttt ctctgtctct tttttattgt cgattcctcg
    70561 tcctgcatgt gcaggtttag caaagatcat gcgtccagca gctgtttgaa gggcacttgt
    70621 tacaacgact tcgatacgtt cattcatgta atgctgtcca tcttctacga caaccatcgt
    70681 gccgtcatca aggtaggcta ctccttgttg gcgttctgtc ccggctttta ctactagaac
    70741 attcatcgtt tcaccaggga tgacgactgg ttttacggca tttgccaacg cattgatatt
    70801 aagaacaggt acattttgga attcagagac tttattcaag ttatagtcat tggttacaat
    70861 gacaccatct aaaagcttag ccaatttgat caatttgctg tctacttcac tgatatcttc
    70921 aaagtctcca tcgtacattt cgacagatat accctcttct ttttgcaagg catttaggat
    70981 atctaatccg cgacgaccac gcacacgttt caaactatcc cctgagtccg cgatgtattg
    71041 tagttcgtaa agaacaaaat tagggatcat caaggttcct tctaagaatc cagtctttgt
    71101 aatatcataa atccgaccgt caatgatcac gcttgtatcc aagattttat atttatggaa
    71161 attttctcct gattttcgtt ctaacagttg actctcaact tgactagctg tttcttgctc
    71221 gatttttttg cttcgtgaac caaaaacttt cttccattct tcgattcgtg tcgttcccat
    71281 tctcaatccc agatagccta agatgatcat cagtaaaatc ggcaatacac tattgacgaa
    71341 tggaatttcc atattgtaaa gaggggtcga gatcacaaca cctaaagtca atccgatgat
    71401 cgcaccgatc gcaccgaaga gaagataagt caagctgacc tcacttagtt tttgttctac
    71461 ttgtttgata atagctgaga tatgtttggc caacagcaaa gataataaaa agaaaataag
    71521 tgcgcctaac aggctgttgg taaatgtatt attcagccat ccgtttgact gttgaccgat
    71581 tgcctgccaa gctgagggta aaaatgaaat acccaagctt gcgccgacaa tgatcatcag
    71641 caaagtaatg acacgttttt gcatcgtttt tcctccgttt cgacaatcat ttattagtta
    71701 gcggaacaca cgcttcaacg tttcagccaa agttgctact ccaactatct cgattccttt
    71761 tggcgcttcc cagttaccta agttgttttt aggtaggtat acttttgtga agccgagttt
    71821 ttggacttct ttgactcgtt gttcaatatg actgacacgt ctgatttctc cagtcagtcc
    71881 aatttcaccg atgaaacatt ccgttggctg tgttcctttt tctttatagc ttgaagcaat
    71941 actgacagcg atagctaaat caatcgctgg ttcatttaat tttaccccac ctgcagcttt
    72001 taagtaagca tcttgattct gaagaagcaa cccagctctt ttttccagca ctgccatgat
    72061 cagagatacc cgattgaaat caagacctgt tgttgttcgt ttggcatttc cgaacatagt
    72121 aggtgtgact aatgcttgga tctctaccaa aattggacgt gtcccttcca ttgccacaac
    72181 gatagcagag ccagttgcac cgtctaggcg ttcttctaag aaaatctgtg aaggattagc
    72241 gacttcttcc aatccatgct catgcatttc aaaaatccca atctcattcg ttgagccaaa
    72301 tcggtttttt actgctcgca aaatccgaaa actatgatgt tgttcacctt caaaatataa
    72361 caccgtatcc accatatgtt ctaacattct tgggccggcg atcgagcctt ctttggtaac
    72421 atgaccgacg ataaaaatgg ctatcccatt tgttttagcg gttttgagta gttctgcagt
    72481 ggtttctctt acttggctga cacttcctgc cacacttgtg atgtctggct gcgtcatcgt
    72541 ttggatggaa tcaatgataa cataatcagg ggagatatgt tcgatggcac gactgatttc
    72601 gttcatatct gtttcagcgt atagataaaa ttctgtgttg atacttccga ggcgttccgc
    72661 gcgtaatttt atctgctctg cgctttcttc gccggataca tataagactt ttccgcctat
    72721 ggctgctaat tgctgagaaa cttgtaacag caaagtggat ttcccgatac ctggatcgcc
    72781 accaatcaag accatagacc ctggcaccac acctccgcct aacacacggt tcagttcttc
    72841 taattttgtt ttgatccgag gttctttttt agggacgacg tctgcgattt ttgttggttt
    72901 tgctttttct cctgtcaaac ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc
    72961 ttcgaccatc gtattccatt ttccgcaatt tgggcagcgt cccaagaact taggcgacac
    73021 atacccgcaa ttttggcaaa caaattgtac ttttgatttt tttgccattg taccgcctcc
    73081 ttttatccta atcatgagac attaaatcaa gcttaagtat ctttaacgtt ttatttactg
    73141 gaagaaccaa atccgccttg acgttgagca gtcgtttgat cttgatctgc ttttaaaaac
    73201 ggcaaaaaga tcccttggcc gatacgctcg ccttttttga tcactacatc cttgaaacca
    73261 aagttggtga attggaacat gatatggcct tcatttgcct cattattgta ataatcactg
    73321 tcaatcacac ctactccgtt tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg
    73381 ttagcaagct gcaaaaattc atcttctccc atgtaagctt tgattccagt aggcacgagg
    73441 gttggtttca attgtttttc attttctgat acatcttttt tcaatccatc tgcgactgtt
    73501 tccaacccct ttttccagcc tgctttccag atacttggaa tcacgacctc ttctgctgct
    73561 tcaaaatcat agccagcagc atgatgtgta gcgcgctgcg gtagattgat attttgatct
    73621 ttatatttag aaataacttc aaatcctctt tttctcaccg gttttcctcc ctaaataaac
    73681 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
    73741 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
    73801 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
    73861 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
    73921 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
    73981 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
    74041 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
    74101 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
    74161 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
    74221 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
    74281 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
    74341 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
    74401 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
    74461 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
    74521 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
    74581 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
    74641 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
    74701 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
    74761 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
    74821 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
    74881 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
    74941 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
    75001 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
    75061 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
    75121 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
    75181 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
    75241 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
    75301 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
    75361 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
    75421 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
    75481 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
    75541 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
    75601 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
    75661 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
    75721 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
    75781 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
    75841 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
    75901 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
    75961 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
    76021 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
    76081 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
    76141 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
    76201 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
    76261 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
    76321 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
    76381 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
    76441 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
    76501 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
    76561 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
    76621 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
    76681 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
    76741 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
    76801 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
    76861 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
    76921 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
    76981 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
    77041 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
    77101 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
    77161 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
    77221 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
    77281 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
    77341 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
    77401 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
    77461 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
    77521 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
    77581 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
    77641 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
    77701 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
    77761 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
    77821 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
    77881 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
    77941 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
    78001 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
    78061 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
    78121 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
    78181 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
    78241 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
    78301 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
    78361 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
    78421 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
    78481 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
    78541 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
    78601 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
    78661 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
    78721 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
    78781 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
    78841 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
    78901 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
    78961 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
    79021 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
    79081 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
    79141 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
    79201 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
    79261 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
    79321 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
    79381 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
    79441 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
    79501 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
    79561 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
    79621 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
    79681 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
    79741 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
    79801 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
    79861 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
    79921 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
    79981 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
    80041 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
    80101 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
    80161 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
    80221 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
    80281 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
    80341 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
    80401 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
    80461 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
    80521 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
    80581 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
    80641 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
    80701 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
    80761 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
    80821 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
    80881 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
    80941 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
    81001 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
    81061 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
    81121 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
    81181 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
    81241 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
    81301 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
    81361 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
    81421 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
    81481 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
    81541 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
    81601 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
    81661 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
    81721 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
    81781 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
    81841 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
    81901 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
    81961 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
    82021 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
    82081 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
    82141 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
    82201 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
    82261 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
    82321 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
    82381 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
    82441 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
    82501 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
    82561 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
    82621 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
    82681 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
    82741 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
    82801 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
    82861 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
    82921 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
    82981 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
    83041 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
    83101 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
    83161 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
    83221 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
    83281 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
    83341 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
    83401 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
    83461 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
    83521 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
    83581 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
    83641 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    83701 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    83761 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    83821 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    83881 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    83941 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    84001 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    84061 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    84121 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    84181 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    84241 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    84301 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    84361 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    84421 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    84481 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    84541 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    84601 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    84661 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    84721 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    84781 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    84841 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    84901 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    84961 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    85021 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    85081 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    85141 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    85201 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    85261 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    85321 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    85381 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    85441 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    85501 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    85561 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    85621 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    85681 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    85741 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    85801 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    85861 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    85921 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    85981 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    86041 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    86101 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    86161 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    86221 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    86281 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    86341 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    86401 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    86461 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    86521 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    86581 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    86641 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    86701 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    86761 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    86821 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    86881 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    86941 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    87001 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    87061 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    87121 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    87181 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    87241 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    87301 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    87361 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    87421 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    87481 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    87541 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    87601 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    87661 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    87721 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    87781 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    87841 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    87901 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    87961 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    88021 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    88081 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    88141 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    88201 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    88261 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    88321 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    88381 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    88441 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    88501 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    88561 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    88621 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    88681 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    88741 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    88801 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    88861 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    88921 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    88981 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    89041 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    89101 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    89161 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    89221 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    89281 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    89341 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    89401 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    89461 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    89521 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    89581 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    89641 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    89701 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    89761 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    89821 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    89881 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    89941 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    90001 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    90061 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    90121 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    90181 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    90241 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    90301 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    90361 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    90421 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    90481 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    90541 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    90601 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    90661 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    90721 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    90781 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    90841 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    90901 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    90961 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    91021 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    91081 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    91141 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    91201 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    91261 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    91321 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    91381 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    91441 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    91501 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    91561 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    91621 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    91681 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    91741 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    91801 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    91861 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    91921 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    91981 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    92041 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    92101 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    92161 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    92221 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    92281 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    92341 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    92401 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    92461 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    92521 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    92581 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    92641 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    92701 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    92761 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    92821 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    92881 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    92941 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    93001 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    93061 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    93121 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    93181 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    93241 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    93301 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    93361 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    93421 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    93481 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    93541 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    93601 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    93661 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    93721 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    93781 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    93841 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    93901 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    93961 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    94021 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    94081 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    94141 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    94201 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    94261 ataaaa
//
LOCUS       DACDKJ010000004        85292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDKJ010000004 DACDKJ010000000
VERSION     DACDKJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 85292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.004"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(23..958)
                     /gene="murB"
                     /locus_tag="IV328_000297"
     CDS             complement(23..958)
                     /gene="murB"
                     /locus_tag="IV328_000297"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8936469.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1031..1951)
                     /locus_tag="IV328_000298"
     CDS             complement(1031..1951)
                     /locus_tag="IV328_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8936470.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2064..2816
                     /gene="xth"
                     /locus_tag="IV328_000299"
     CDS             2064..2816
                     /gene="xth"
                     /locus_tag="IV328_000299"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8936471.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3680..4213
                     /locus_tag="IV328_000300"
     CDS             3680..4213
                     /locus_tag="IV328_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8936472.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4703..5227)
                     /locus_tag="IV328_000301"
     CDS             complement(4703..5227)
                     /locus_tag="IV328_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8936473.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5220..5693)
                     /gene="tsaE"
                     /locus_tag="IV328_000302"
     CDS             complement(5220..5693)
                     /gene="tsaE"
                     /locus_tag="IV328_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8936474.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5816..6796)
                     /gene="pta"
                     /locus_tag="IV328_000303"
     CDS             complement(5816..6796)
                     /gene="pta"
                     /locus_tag="IV328_000303"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8936475.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6949..7629)
                     /locus_tag="IV328_000304"
     CDS             complement(6949..7629)
                     /locus_tag="IV328_000304"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8936476.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7755..8624
                     /locus_tag="IV328_000305"
     CDS             7755..8624
                     /locus_tag="IV328_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8936477.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8650..9165
                     /locus_tag="IV328_000306"
     CDS             8650..9165
                     /locus_tag="IV328_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936478.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9212..9775)
                     /locus_tag="IV328_000307"
     CDS             complement(9212..9775)
                     /locus_tag="IV328_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8936479.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9830..10009)
                     /locus_tag="IV328_000308"
     CDS             complement(9830..10009)
                     /locus_tag="IV328_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936480.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10086..10595)
                     /locus_tag="IV328_000309"
     CDS             complement(10086..10595)
                     /locus_tag="IV328_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936481.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10691..11071)
                     /locus_tag="IV328_000310"
     CDS             complement(10691..11071)
                     /locus_tag="IV328_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8936482.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11401..12150
                     /locus_tag="IV328_000311"
     CDS             11401..12150
                     /locus_tag="IV328_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8936483.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12334..13611)
                     /locus_tag="IV328_000312"
     CDS             complement(12334..13611)
                     /locus_tag="IV328_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936484.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13690..14334)
                     /gene="pcp"
                     /locus_tag="IV328_000313"
     CDS             complement(13690..14334)
                     /gene="pcp"
                     /locus_tag="IV328_000313"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8936485.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14533..15477)
                     /locus_tag="IV328_000314"
     CDS             complement(14533..15477)
                     /locus_tag="IV328_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8936486.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15479..16165)
                     /locus_tag="IV328_000315"
     CDS             complement(15479..16165)
                     /locus_tag="IV328_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8936487.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGIASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16639..16959
                     /locus_tag="IV328_000316"
     CDS             16639..16959
                     /locus_tag="IV328_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8936488.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17113..18018
                     /locus_tag="IV328_000317"
     CDS             17113..18018
                     /locus_tag="IV328_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8936489.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18015..18788
                     /locus_tag="IV328_000318"
     CDS             18015..18788
                     /locus_tag="IV328_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8936490.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(18969..20393)
                     /locus_tag="IV328_000319"
     CDS             complement(18969..20393)
                     /locus_tag="IV328_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8936491.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            20604..21050
                     /gene="nrdI"
                     /locus_tag="IV328_000320"
     CDS             20604..21050
                     /gene="nrdI"
                     /locus_tag="IV328_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8936492.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(21115..23097)
                     /locus_tag="IV328_000321"
     CDS             complement(21115..23097)
                     /locus_tag="IV328_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8936493.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(23111..24007)
                     /gene="murQ"
                     /locus_tag="IV328_000322"
     CDS             complement(23111..24007)
                     /gene="murQ"
                     /locus_tag="IV328_000322"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8936494.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(24010..25071)
                     /locus_tag="IV328_000323"
     CDS             complement(24010..25071)
                     /locus_tag="IV328_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8936495.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(25282..25809)
                     /locus_tag="IV328_000324"
     CDS             complement(25282..25809)
                     /locus_tag="IV328_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8936496.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(25876..27378)
                     /locus_tag="IV328_000325"
     CDS             complement(25876..27378)
                     /locus_tag="IV328_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8936497.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(27636..28757)
                     /locus_tag="IV328_000326"
     CDS             complement(27636..28757)
                     /locus_tag="IV328_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8936498.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            28883..29824
                     /locus_tag="IV328_000327"
     CDS             28883..29824
                     /locus_tag="IV328_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8936499.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(29974..30870)
                     /locus_tag="IV328_000328"
     CDS             complement(29974..30870)
                     /locus_tag="IV328_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8936500.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            31139..32479
                     /locus_tag="IV328_000329"
     CDS             31139..32479
                     /locus_tag="IV328_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8936501.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            32697..33140
                     /locus_tag="IV328_000330"
     CDS             32697..33140
                     /locus_tag="IV328_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8936502.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(33644..35062)
                     /gene="nhaC"
                     /locus_tag="IV328_000331"
     CDS             complement(33644..35062)
                     /gene="nhaC"
                     /locus_tag="IV328_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8936503.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(35465..36688)
                     /locus_tag="IV328_000332"
     CDS             complement(35465..36688)
                     /locus_tag="IV328_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8936504.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(36719..37558)
                     /locus_tag="IV328_000333"
     CDS             complement(36719..37558)
                     /locus_tag="IV328_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8936505.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(37575..38255)
                     /locus_tag="IV328_000334"
     CDS             complement(37575..38255)
                     /locus_tag="IV328_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8936506.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(38257..39330)
                     /locus_tag="IV328_000335"
     CDS             complement(38257..39330)
                     /locus_tag="IV328_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8936507.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(39977..40648)
                     /locus_tag="IV328_000336"
     CDS             complement(39977..40648)
                     /locus_tag="IV328_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8936508.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(40735..43413)
                     /gene="mgtA"
                     /locus_tag="IV328_000337"
     CDS             complement(40735..43413)
                     /gene="mgtA"
                     /locus_tag="IV328_000337"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8936509.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            44072..44509
                     /gene="ndk"
                     /locus_tag="IV328_000338"
     CDS             44072..44509
                     /gene="ndk"
                     /locus_tag="IV328_000338"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8936510.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(44531..45397)
                     /locus_tag="IV328_000339"
     CDS             complement(44531..45397)
                     /locus_tag="IV328_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8936511.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(45557..47650)
                     /locus_tag="IV328_000340"
     CDS             complement(45557..47650)
                     /locus_tag="IV328_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8936512.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(48399..49022)
                     /locus_tag="IV328_000341"
     CDS             complement(48399..49022)
                     /locus_tag="IV328_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936513.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(49181..50578)
                     /locus_tag="IV328_000342"
     CDS             complement(49181..50578)
                     /locus_tag="IV328_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8936514.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(50610..51581)
                     /locus_tag="IV328_000343"
     CDS             complement(50610..51581)
                     /locus_tag="IV328_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8936515.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            51955..52380
                     /locus_tag="IV328_000344"
     CDS             51955..52380
                     /locus_tag="IV328_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936516.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(52868..53569)
                     /locus_tag="IV328_000345"
     CDS             complement(52868..53569)
                     /locus_tag="IV328_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8936517.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(53585..54025)
                     /locus_tag="IV328_000346"
     CDS             complement(53585..54025)
                     /locus_tag="IV328_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8936518.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(54025..54600)
                     /locus_tag="IV328_000347"
     CDS             complement(54025..54600)
                     /locus_tag="IV328_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8936519.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(55448..55927)
                     /locus_tag="IV328_000348"
                     /pseudo
     CDS             complement(55448..55927)
                     /locus_tag="IV328_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(56086..56787)
                     /locus_tag="IV328_000349"
     CDS             complement(56086..56787)
                     /locus_tag="IV328_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8936520.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(56895..58304)
                     /locus_tag="IV328_000350"
     CDS             complement(56895..58304)
                     /locus_tag="IV328_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8936521.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(58352..60217)
                     /locus_tag="IV328_000351"
     CDS             complement(58352..60217)
                     /locus_tag="IV328_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8936522.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(60338..61189)
                     /locus_tag="IV328_000352"
     CDS             complement(60338..61189)
                     /locus_tag="IV328_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8936523.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(61499..62212)
                     /locus_tag="IV328_000353"
     CDS             complement(61499..62212)
                     /locus_tag="IV328_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8936524.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(62280..62606)
                     /locus_tag="IV328_000354"
     CDS             complement(62280..62606)
                     /locus_tag="IV328_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8936525.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(62729..62956)
                     /locus_tag="IV328_000355"
     CDS             complement(62729..62956)
                     /locus_tag="IV328_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8936526.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(63278..64531)
                     /locus_tag="IV328_000356"
     CDS             complement(63278..64531)
                     /locus_tag="IV328_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8936527.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            64592..65332
                     /locus_tag="IV328_000357"
     CDS             64592..65332
                     /locus_tag="IV328_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8936528.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(65418..66176)
                     /locus_tag="IV328_000358"
     CDS             complement(65418..66176)
                     /locus_tag="IV328_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8936529.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(66173..67201)
                     /locus_tag="IV328_000359"
                     /pseudo
     CDS             complement(66173..67201)
                     /locus_tag="IV328_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(67221..70388)
                     /locus_tag="IV328_000360"
     CDS             complement(67221..70388)
                     /locus_tag="IV328_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936530.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(70612..71496)
                     /locus_tag="IV328_000361"
     CDS             complement(70612..71496)
                     /locus_tag="IV328_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8936531.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(71591..72028)
                     /locus_tag="IV328_000362"
     CDS             complement(71591..72028)
                     /locus_tag="IV328_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936532.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(72245..73156)
                     /locus_tag="IV328_000363"
     CDS             complement(72245..73156)
                     /locus_tag="IV328_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8936533.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(73613..74041)
                     /locus_tag="IV328_000364"
     CDS             complement(73613..74041)
                     /locus_tag="IV328_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936534.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(74069..74707)
                     /locus_tag="IV328_000365"
     CDS             complement(74069..74707)
                     /locus_tag="IV328_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936535.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(74711..76747)
                     /locus_tag="IV328_000366"
     CDS             complement(74711..76747)
                     /locus_tag="IV328_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8936536.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            76873..77538
                     /locus_tag="IV328_000367"
     CDS             76873..77538
                     /locus_tag="IV328_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8936537.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(79473..82622)
                     /locus_tag="IV328_000368"
     CDS             complement(79473..82622)
                     /locus_tag="IV328_000368"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP8936538.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(82635..83852)
                     /locus_tag="IV328_000369"
     CDS             complement(82635..83852)
                     /locus_tag="IV328_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8936539.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(83842..>85292)
                     /locus_tag="IV328_000370"
     CDS             complement(83842..>85292)
                     /locus_tag="IV328_000370"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8936540.1"
                     /translation="ADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIA
                     PEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRN
                     VTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQI
                     VTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLIL
                     HGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGK
                     LAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIG
                     MPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYA
                     ERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVL
                     EKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT        24703 a        17666 c        14566 g        28357 t
ORIGIN      
        1 ttattttaag ttccattact tattaattgg ctaaacttct gcttgtttct gatattgctt
       61 gtttttctcc aatgatccgc acttctgttt ctaaagacac atcaaatttt tctttgatta
      121 cttcttggat gtgcgcaatc aattcaatat aatctgtcgc tgtcgcatga tcgatattga
      181 cgatgaatcc agcatgtttt tcagagactt gtgcaccgcc ccatttcagg ccttgcaatc
      241 ctgcttgttg gatcagctga cctgtatagt gtcctactgg gcgcttaaag acacttccgc
      301 atgaagggta ttctaatggc tgttttgatt gacgcaattc ggtcaactca tccattcttg
      361 ctttgattgc ttcataatcc cctgtttgca aactgaatcg agcttccaat acgatagcac
      421 gtaattcttg gactttactg tgacgataag aaaataccat ttcgtctttt gtcaaagttt
      481 tcaatgttcc atcttcaagc aaaagatcca cttcagcaaa aacatctttg atctctccgc
      541 catacgcacc tgcattcata taaaccgctc caccgacact tcccgggatg ccgcaagcaa
      601 attcaaatcc agtcaatgat tctgccaaag ctgcataagt cgtatcaatc agtgaggcac
      661 ctgcttccgc aatgaccatc gttcctttga catgaatctg tttcatttca gtcagcatga
      721 tgactacacc gcgaatcccg ccatcttgaa cgatcagatt gcttgcattt cctaatacca
      781 tccaaggtat atcatggatt cggcaataat cgatgatttg ctttacttct tcttgttttt
      841 tcgggaatgc aaggacatca gctggtccac ctgttttcgt aaacgtatag ttcatcaatg
      901 gttcatcaaa caatatagta atcgtggcta agctttcaac tatttcttct ttattcactg
      961 aggattttcc tttcttttct caaacgtcct cattattcta tcacgcttaa tacaatcttg
     1021 ccagtcttgt tcaaagttga tgacggtagt gatctttaag cacttcctca cataattcat
     1081 gacttcttac aacttctttt tgtttaagca attctgtttc cgatttacct aaacggatct
     1141 gagaaaagaa ggcctctacc attggctcaa acccgcgttt gactagcgtg tttgtccagt
     1201 cgccaaattc tttcactttt tttccatcag cctcttgaat gacaagctgg gtcagatcgt
     1261 ttacttcata agtaccattt ttacttgtta cttgatattt ttctatatta gcgccacttg
     1321 ctaaatccat cgttaaaata gccgtttgat tttgtgtttc taattgtaaa atggcatatt
     1381 ccaaaaattc ctttgtttct cttatctggt attttgttct caaaatgggt tcatctaata
     1441 aatacacagc tgtatcaata agatgtaaaa acaaatcata aaggacgaac tctgtcgttt
     1501 cttttgccgc tattcgattt ttttgcaggt ggatcaaccg cttatctggg atacgtttca
     1561 attcttctac cataggagca aagcgccggt tgaaaccgat catcaaaatt atttggttct
     1621 tatcagctaa ttcttgcaat tcacgaactt catttagatg gacactcagt ggtttgtcaa
     1681 tataaacatg gataccattt tctagacatt ttttggctaa ttcataatgt acttttgttg
     1741 ctgcgtggat catacatgct tcgatttttt cttctaaaag ctcatcgatc gtttcatata
     1801 gatgttggaa cccgtactct ttttgaattc tttcttttgt ttctttattt cttgttgcaa
     1861 aataaaaatc aagtgttcct tgttttttcg cataagttgg taaatatgct ttttctgcga
     1921 tattccctac accgattaca ccaatcttca tctactttcc tcctggatca tttagttact
     1981 ttcatcttac accatttttt actaaaatga ggtacaatag tatcgcaaag gtaaggaaat
     2041 taattcgaag gagtgtgctt ttattgaaat ttatctcttg gaacgtaaac gggcttcgtg
     2101 ccatcgtaaa taaaaatttt ttagaagttt ttcatgagtt agacgccgat tttttctgtc
     2161 tgcaggaaac gaaactccaa gcaggacaaa ttgatttaga tcttccaggt tattatcaat
     2221 attggaatta cgcggaacga aaaggctatt ccggcacagc gatttttgct aaaaaaccag
     2281 cattgaatgc cacttacgga atgggtatcg atatccatga tacagaaggc cgtctgatta
     2341 ctttggaata ttccgatttc tttcttgtaa cctgttacac gccaaattct caaagcgaat
     2401 taaaacgttt agattaccga ttagaatggg aagaagcctt ttataattat ttggaaaatc
     2461 taaaaaaaca aaaaccagtc atcgtgtgtg gggatctaaa tgtcgctcat caaaagattg
     2521 atttgaaaaa ctggaaaacg aatcaaaaaa atgctggatt cactccagaa gaaagagcgg
     2581 ctctttctcg actgttggat aacgggttta tcgatacatt ccgttatttt tatccaaccc
     2641 aggaaggtgt ctactcttgg tggaactacc gattcaattc aaggaaaaac aatgctggtt
     2701 ggcggatcga ttactttttg acgagcaaag atttagagcc aggactagct gatgcaaaga
     2761 tccatacgga cattatgggc agtgatcatt gtccagtaga attagatttg aaataaagag
     2821 gttgtgaaaa agtcgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
     2881 cattttgcat aaatttggca taataccgag aaactggctt ttgaacactg tttatcgaga
     2941 gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     3001 atatttttcg tttggttggg cttattaccg cagatacaag cttttgaaca ccgtttatta
     3061 agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3121 ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
     3181 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3241 ttcctcatat ttttcgtttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
     3301 ttattgagaa gttgtgaaat cagcgtcctg acctgagtat tagagaaaca aatggcaaaa
     3361 tagttcctca tattttgcta ttttttgagc taataccgag ggtcagctga ttgaacaccg
     3421 tttagtggag aagttgtgaa aaagtcgcct actttcgagt cgtaagaaat ttttttgaaa
     3481 atggttcctc acaactttgc ataaatttga cttaataccg agaagtcatg atccccatct
     3541 aactccaaat cacaaggaat gagttcaaaa actagctttt ctcaacaaat attacataaa
     3601 aaattctatg ttttttgtta aatttatttg cttttttcaa aagtatgtta cagtaatttc
     3661 ccaaggaggt tttacagaaa tgaattttgt tgccatggat tttgaaactg ccaatcatca
     3721 gccttatagt gcttgttctc ttgcgctagt catggtaaaa aatagccaga tcgttgatga
     3781 attctataca ttgatacagc ctgagactcc ttttttctgg cgaaatgtcc aaattcacgg
     3841 aatccatcaa gaagacgtac gcaatgcgcc gaaattccca gaagtctggc aaactatcca
     3901 accatatttt caaaaaaatc gtttagtcgt tgcacataat gctgcttttg atacaaaggt
     3961 cttagcggga tgcttagatt attatcatct tgaacagcct aattatcttt ctttatgtac
     4021 agtgaaaagt agccgtcgcc tgtttcctga atttccaaat catcgattga acactgtgtg
     4081 cgaaaatttg aacatccaat tagatcatca ccatgatgca ttggaagata gtcgtgcatg
     4141 tgcggaaatc ctgctttatc aagaaaaaaa attcgggaca gatccattga aaaaacttgt
     4201 gactataaag taaaagaata cttatgaaac acacaaagag gtcgtgagcc tgccgtttca
     4261 ctccgagtca caaggagcaa tttcaaaaaa tagcttctca tgtttattaa atattgtgac
     4321 tggtgaccga ggagctggca ggagaacacc gtttattgga gaggttgtga aaaagctgtt
     4381 ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt catttggttg
     4441 gacttattac cgcagatgca agcttttgga caccgtttac caagaggttg tgaaatcagc
     4501 gttttgacct gagtattaga gaaacaaacg gcaaaatagt tcctcatatt ttgccgtttt
     4561 ttgagctaat accgaaggtc agctgattga acaccgttta ttcaaaaaaa atggactgcg
     4621 acacgacttt tgtcacagtc ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt
     4681 tctcactctt ttcctatttc ttctattcaa tcaaataact cattttatat acatctaaaa
     4741 attcacctag atctgttctt gcccctctag gcatgaccgc ttcgatttgg aagcccattt
     4801 ttcggtaaag gtgtacggct ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa
     4861 gtacacccat ttccttcgcc caactgatga tttcttctag cataagagtg cctagtccca
     4921 ttccccaata ttcctgcaga atactgatac cgacttctcc cacgtgagac aaacgaaatt
     4981 gactgtctgc cttgacagat gctgttccaa taatcgtccc ttcatagatt gcaagcagca
     5041 ataaattatt attgctttcg tatatataat ccaactcttc ttctaaggtt tctggtggta
     5101 acaggagttc tgcttcatcc aaaactaaaa attctgtctc cttattaact tgtcccatca
     5161 tcaaaagaat gtcttttgca tctcctggca ctgcttcccg caagatgatc ccaacttctt
     5221 cactcatgat tttcagccca tttttctaga attcgctgtt tgaaggtttc actttgctcc
     5281 ccgtatgaga ttagtttcac ataccgcttt tctaaatctg tatcactttt ttctaaaatc
     5341 agttccaaat aatcctccgg cagtgcttct tctaatagat ttccccattc aacaacggaa
     5401 agcccatcac cttcaaaata ttcatctaac cctaaatctg ctccgctagc tgctacccga
     5461 taaatatcca tatgatacaa cggaatccga ccttgactat attctctaat aatcgtgtat
     5521 gtcggacttt tgatcatttg cgttatccct aatccttggg caattccttt cgtcaacgtt
     5581 gttttacccg cacctagatc accagtcaat atcagattgt cacctggcat tgcggaatga
     5641 ccaattgcct ctgcgaattg ttctgtcatt gttaatcctg atagttcgat catttttcat
     5701 cttctcccgc tcttctttct ttttgccagt atatcgcgta atgaagtttt tttaaagaaa
     5761 aaatgaacta gtcaagaaca gcagctgctg tttttggcta gttcgcgtgt atttgttaat
     5821 caatcaatgt ttgtgctgct gtgataatcg ataatttata tacatcttct tcatttgctc
     5881 cacgagacaa atcagaaacc ggtttgttca agccttgtag aattggtccg attgcttcaa
     5941 agttaccgaa acgctgagca attttatagc cgatatttcc tgactgcaat tctggaaata
     6001 caaagactgt tgcttgacca gctacttctg aatctggcgc tttcagttgt gcaactgaag
     6061 gaacataaga agcatcaaat tgcaattcgc cgtcgatcat gtattgtggc gctagttttt
     6121 tagcgatttt agttgcttca actactttgt ctacttctgg cgatttcgct gagccttttg
     6181 tcgagaagct catcattgct acttttggtt cgatatcgaa caattctgct gtttttgcag
     6241 aatctacggc gatttcagcc aattcttgcg cagttggatt cacgttgatc gcacaatccg
     6301 aaaagacata tttttcttga tctcttccac gtaccatgat catcgctccg cttgtccgac
     6361 tgatgccagg ctttgttttg atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat
     6421 ggatcgctcc agagaccatt ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt
     6481 aattcacatc ttttaaaatc ttttctgctt gttctttgga tgcctttcca tttctacgtt
     6541 caacaaaagt atccaccatt tcatcccact tatcgtaatt atcagggctg atgatcgtaa
     6601 agctagatgt tttgattcct ctggcatgcg ctgcatttct tatttcttct tcgttcccta
     6661 ctaaaatggg ttccacaagt tcttctgctt tcaaccgggc agctgcgcct aaaatacgag
     6721 catcagtagc ttccgggaaa acgattttga tcccacggcg aacaatttta aatcttaaac
     6781 tatcaaacaa ttccacaaga tgacctccag acgtttttat atgtattatc atacactatt
     6841 ccagaaaaaa agaacagttt attttatctg tatcagtcaa aaatgctgga aaaattaagt
     6901 ttttcacata caaagcgaac tgcactataa caggaaagcg ctgttttatt acactgtttc
     6961 aggcagttgc caatcaatcg gttcttctcc taacgcaagg agcgcatcat tcgttttcga
     7021 gaagggtttg gaaccaaaaa agccacgact tgcagataac ggactgggat gtggagatgt
     7081 tatgatgata tggcgactct tatcgatcat tttgattttt tcttgtgccg gttttcccca
     7141 caagataaac acgattggtt tttcccgttc atttaatttt tcaatgatcg tgtctgttaa
     7201 tctttcccaa cctttcccgc gatgagaata cgcttgccct tcccgaacgg taagaacggt
     7261 gttgagtaaa aggactccct gtttagccca tgacactaga tttccatgtt gaacaggtgc
     7321 gattccaaga tcatcataaa gctctttata gatatttctt aacgatggcg ggatctttac
     7381 gccaggttgc acagaaaaag ataagccatg agcttgattt tctccatgat aaggatcttg
     7441 tcctaaaatc accactttaa cttcttcata aggagtcaat tccaatgctt catagatatg
     7501 atacatatct ggatggatct tttgggacgc atattctttt tttaaaaatt ctcttagatg
     7561 cagataataa tcttttgaaa attctgagga caaaacttct tgccaactgt tatgaatgat
     7621 ttctttcaaa aactccacct cctaataaaa agaataactc acattttctt caatcacaag
     7681 ggacaatctt caccctcagc acttgattaa tatggtaaac taggaacaag aagcagaaag
     7741 gaacgataat cattatgatc aaattaattg catcagatat ggacggcaca ttattagatg
     7801 cccatatgag tgtttctcct gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg
     7861 ttgagttcat ggtcgctact ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg
     7921 taggaatcga ttgtgcgatg atcactttga acggggcaca ggtatttgat aaagaaggaa
     7981 attcattgtt taccgtgtcg attccaaata atcaagcaat ctctgtttta gatattctag
     8041 atgctaacgg tatttactat gaagtagcaa ccaacgatgg tctttattct gaaaatcaag
     8101 cgaaacgaat tgaaagtttt gcttccatgg tggctaccca tttacctcac cttacgtata
     8161 aaatggcgat tgccatggcc tcagctaagc tggagttatt gcacatcaca tacgtggaca
     8221 gtattcgtgc tattttggaa gaaaagaatc tggaagtttt aaaaattatt tgttttcata
     8281 cagaagggcc agctgtatta ggtccagttg gaaagcagat ttctctttta ggcgatctag
     8341 ccgttacttc ctctggtcaa aacaatatcg aagtgaacca taaaaacgct caaaaaggaa
     8401 ttgctgttgc ccatgtcgct cacgagcgtg ggatttcttt agaagaagtc atgacgattg
     8461 gcgataactt caatgatgtc agcatgctgc aagtagcagg agtcagtttc gcaatgggaa
     8521 atgcagaaat cgaagtaaaa gattatgcca aatatacaac ggacacaaac cttgaatctg
     8581 gcgtaggaaa agccatattg cgcgcgattg cagaagactt gtagttttat ctagaaaaga
     8641 gggatataga tgtcagaatt tttcattcag gaaaagcagc ttggacgtgc aactcggaca
     8701 attgtcaaaa acgaaaaagg acattccctg tttttgatgg ttggtcgctg gggaacaaag
     8761 ggcgatgcat tgtctcttta tgccatgaat ggcgatctcg ttgctcacat caagcagata
     8821 agcctgacat tcggtacgcg ttttgagctg tataaagagt tcgaaaaggt gggaaccatg
     8881 cagaaaatat tcaattggcc cggtgacttt tattatattc gtagacttca ctggacagtc
     8941 caaggtgata tctataatca tcaatatcaa atccatcatt tcaatcagca aatcatgaaa
     9001 atggataaag caacactttt ttcaggagat tactatgtat tggatgtctc aaatgatgaa
     9061 gatgctccag tttgtatttg tattgctgcg gttttagatt actggctcta ccgtaaagac
     9121 aaaagtcgcg ataagcctgt ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa
     9181 atgcggaaat acgcatttca ggtacatctt tttagatatt cttttgcata tgatcataaa
     9241 gatgaccact gatcatccgc tgaccgacaa ctttgaagtc atggcgttcg tataattttt
     9301 tagcctttgg atttttttca tccacactta gaccgatgac ggagcgatta gctttcttcg
     9361 ccaatttagg taatgcttct aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg
     9421 atacactgat agagtccaaa taccattcgt tgggaaacgt ttctttatca atgaacaaac
     9481 gtgtagacgc atctagctga tatttctcta agataggtac cagtggctga tcgatgattg
     9541 attcttcttc atctgtataa ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta
     9601 cgatacctcg tgaataactg taacgataat ttggatcagt gattgcttct tctaaaatct
     9661 ctaaagtctt ttgttcccct gtctgcttca aaaatggcag ttccatatct tttagaatca
     9721 ctaagatcaa tggggcgatt tctggagcat cttcttttct cgcaaaacga atcatagttt
     9781 cctctccttt tctccattct aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa
     9841 aatcttcaat cttttcagta atgaaataac catgagtaac gattcttgcc gcttcagttc
     9901 ggcaataagg gcagacgact ggcatttctt tatcattatg ttcgactaca ttattgactt
     9961 cttcgtatgc aaattttttt ccgcaattag ggcatttcat tttttccatg ttatctcaac
    10021 ttcctttcat ctttactctc ttagcttact cttatattgt aaaaaagaga agcaatatcc
    10081 ttcttctatg cagatatcca tacctgacgg tcctgttctc catctattaa tcggacttta
    10141 taataaggac gttcatcttt gactagaagt gtccactctt tcacttttgc ttttttcccg
    10201 tagtagctga ctgcttcctt catcgctgct tgcggttgtt tgaactcttc cgttttttct
    10261 atatcaaaaa gatgttctgc ggccttttcc gtgtcatttc gtacggttgt tttcccagtg
    10321 gatggattga ctgctatgac ttgtccttca caaaaaaact ggtattcata tgttgcattc
    10381 gtaggattct ccaaatcttt ttcaactgca aaatgaatag cagatatgtt atgttctttt
    10441 gtttcgtttt ttacgattgc tgctgctttt ttcaatgaga tccgatattc ctggtcacta
    10501 atatcatcca tgtgaaaagc ggcattgagc agtcccgtca taccggtaat ccctattacg
    10561 atgagggcag caacaccaaa catcattttt ttcattcttc attctcctgt ttcttttttt
    10621 taaagaatat caagaaatga acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat
    10681 tttgttatct ttattcgtgc agttctaatg gcaatccgtc tggatcaaag aaaaacgtca
    10741 ttttttttcc tgtaaaggta tcttctctaa tcggttctga ttcgatccct aaggactgca
    10801 attctgcaat tgtttcttca atatcgtcta ctttgaaagc aagatgacgc aagccacaag
    10861 cttctggata gtttggtcgt tgtggcgcat tagcaattcc aaaaatctcc aattcagcac
    10921 ttcctagttt cagatctaat ttatagtcat ctcgttcttt gcggtaattt tctctgatta
    10981 cttcaaatcc taatttatta acataaaaat cacgtgattt ctgataatct gaaacaatga
    11041 tggctatatg atggatcgtt gtaaggttca tatgagcact cctattcttc gtttaagctc
    11101 ggatctatat taatctccag ttccattgta aatgtgtcag attgaaaaaa atagctgttt
    11161 ttccttttta taagaaaaaa ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc
    11221 ctgttcccaa tataataaat gaaagtaaga aattatattt tcaagaaaac aaacagaaaa
    11281 ttatcataac acaaattatt tataattgaa agactagttt tttttcaaga aaagcataca
    11341 atagattgtg ttagtaaata cagaacacac tacatttagt gtcttaagga gtcctcatac
    11401 atgaagtcat attttcagtt tttagctcat cagctaaaag gctggccgca acaaaattat
    11461 tatcttttct ttttcagtct tggctgccag gtcatgactc ttgtcaatag tcccatcact
    11521 tggctgtccg ttattacatt tatcggtacc acgcttggtg tattgtgtat tttatccatc
    11581 aatgcggcaa aatcagtcaa cggtatttta ggtattttgt ctgcgatttg ttttattatc
    11641 gttgggtttt cagcaaagaa ctatttaagt atcggtgaac agattgctta tatgatcaca
    11701 ttggatatcc ccgtattgtt aagtaaagaa tggaatcata atatggcttc aaaaatccga
    11761 aaattcactg gaaaaagctg gtttatcgca atcatcagta ctttccttgt ctatcttgtt
    11821 tcaggattcc tgattggtcg ttatacagat gatcctcggc cgtgggtaga tgccattgct
    11881 ttttctatta gcttgactgc tggtatcatc agtttcttgc gttacaacaa ccaatattac
    11941 tggtggttag cttcagggct tgctcaaatg gtcctttggt atatttcatt tacgcatgga
    12001 tctgcaagtt tggcgatgtt cgtgaacagc tctgtttact tgctgaatga tgtccttgca
    12061 tttaccgttt caccttggta taaccaaaaa gaacgtgaac gtatgcaaaa aatggaagaa
    12121 gaatactaca acgcacaaca acacgcataa aaaaactgag cgtgggacaa aagtaaagaa
    12181 cacttttgtc tcacgctcta aacacgtata aacggcggaa tcagaagcaa ctccttcgga
    12241 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
    12301 ggagttaaac gcttctgtcc cagcctctat tttttatagt aattccttca gatactttct
    12361 tgcctcatca ttgaaacgca gtgattcttc taacttctga taatattcca acgcttgttt
    12421 ctgagcttca gtaaaacgat aatgttttcc tttccatttg tcgattgtct cttgcacatt
    12481 tccagattgg atatctttca ctactagttg ccatttttct tttgagactg tacggtcttt
    12541 cttatcagca gattgtatag cttctcgttc tttacgcaat acttttaaaa aaccatttct
    12601 gactatccga ggaatgctgt cgaagaaccc ttctgtaaaa ccatagcgac aatattgctt
    12661 ccaaataaat agatagattt ttggttggat gaatcgttgg tttttcgcaa aatattccat
    12721 ctgagcagtg acttctggtg ttcgttgacg ttttgtcttt ctcaacacgg aaataaatgt
    12781 ttcaaaataa gcattaggat gtactggcac ttcttcatat acgatggatt gccaatcttt
    12841 acgtaaaaat gcgcgatagt tgatcaagaa ttcttctaca tcctttacta attcttctgc
    12901 aaagtcgtca aatagttctt tttttatctg tcgctttttt gactgcccat tgatgtgttc
    12961 acttattttc ttgatttcat tttccaatgc ttgataatcc gctatgtaaa tcggcatttc
    13021 tacttggcga aattctgcga gccgctgagc taattttata tccggtaaat atggctgttt
    13081 catccgacga ttttggtaca agacaaaaca gtatttttgc catagtcctt caggaaaatc
    13141 gatattcttc tgcttaagcc ataataattc gtctaaggcc agatcaactt ttgtcattcc
    13201 ctgatggatc gaagctgcca ctgtcggaga gacatgcagg tgatcggcaa agtggttgat
    13261 gcctagtttt aggatctttc cggtttttgg cgatttaacg atgtattgca cttttaattc
    13321 tctaccacac tcacaataca atttttgctg gcttcgtttt gttcgaaaaa acggattgat
    13381 cttgaagtca gaaaacaccc aatctgttgc agctaaataa tgttcttcca taaaacgaga
    13441 gctgattaga tatcggcggt gcttatcgac tagttcccgc tgttttttcg ttaattttgc
    13501 atagatctcc caacgttgac tgcttgtgta ttgtttagaa attttgatgg gatagctttt
    13561 gctgatttct tttggaaacc attcttcaaa gacggattgc cattcttcca cggctgtttc
    13621 tttcctttct aattacaaaa agctggctac taacataacg ttagcaacca gccagtgttt
    13681 cttgctaaat tagtgagttg ttccggcatt tccggaaata tcttctttat tttctactgc
    13741 tgcttcaact gcataagtca aagaagaagc aatgtcttct aaagacatag aaggcgtacc
    13801 cactggacga tcgatcactt gatcgggtga gaatggcacg tggatgaacc cacctcgaat
    13861 tgccgggaat tgtgtttcga tcaaatgaag caaacgatac atgatatcat tgcagacaaa
    13921 tgttcctgct gtataagaga tgtacgctgg tagaccatga tcttgtacat tttttgtcat
    13981 tgctttgatt ggcaatgagg taaagtaagc ggctggaccg ttttcttcta aagccgtatc
    14041 gaatggctga tttccttcat tatcagggat tcttgcttct gctagattga ttgcgacacg
    14101 ttcgaacgag actgctgacc gcccacctgc ttgtccgaca cagatcacga catctggttg
    14161 gtgctgttgg atggcttctt tgactacttg actgcttttt tcgaatacgg tcggtacttc
    14221 cactttgatg atttcagcac cgctgatttc atctggcatt ttttttactg cttcataggc
    14281 tggattcact ttgtccccac caaatggatc aaaacctgtt actagtactt tcatctgttg
    14341 ttcctccgtc cgattgattt aacatatggt tatcttttat cataccattt tccttgaaca
    14401 gcgtaaataa aggaaacaat agtttaaata acataacaga tcaaacttaa tatattctta
    14461 taaaaaaata gtgttccact acaatgttag aagaggttgc gacaattatt ctgtcacaac
    14521 ccctctcttc tattatttaa atagaatcat ataagcaatt tggaaaacta gcataaataa
    14581 cgcaataaac aactggtttt tgatcactcc gtatttgtct ttcatctcca gcatcgctac
    14641 aggcacgata ttgaagttag cagccatcgg tgtcagcagg gtcccacaaa aaccgcaagt
    14701 caatgcaacc attcctatta acgctggatt cgctccatga ctaaatacga atggtgcacc
    14761 gatcccaacc gtcatcacgg tgatggccgc aaatgcattc cccatgatta ccgtaaatat
    14821 caccatgcca agagcgtaaa tgatgatgcc gatgttcaca tttccttctg gaacgatcgt
    14881 acctactgca ttggaaatca ctgtcccaac tccagcgctt gtaaataccg cacctagaga
    14941 agctaaaagc attggcaaca tgcttaacgg ccctactgtg cccagcatat ctgctgcatc
    15001 atctaaaaat gtacttggtc gattatctcg agaaaagaac atcaagatta gcatggctgc
    15061 aaaaacaccg acgcccacgc cgactaaagc acctaaattg gtaaatagag caaacaagat
    15121 cgcaaagaca ccgatactca aagctggaat aaaaattttc attcctagtt tgtctgacat
    15181 tttttccata tattcttttg atggaagccg gctttctcct ttgctgactt tcttagcaat
    15241 tgctggtatg gtcatcgcaa agatcaagcc accgttcacg attgccggaa gccatcttcc
    15301 tcctgcaata actaccccta agaccgtcca gaaaaaggct gacccataac gatgaggatt
    15361 ggttttatct gttaaatttc ttactccagt gtaaatcaat acgagtccca tcacgatata
    15421 aaggatctcc agtaatttat cgcctaataa aatatcagga tttgtaaaaa agtccatctt
    15481 attgcccctt tcctggataa tatttcttcg ctaatttctt ttctttaaga tagaagtaga
    15541 cacttgccac aactaaagcc gtgatagcca ctggaacttc cactaaagca agctgtccca
    15601 atgttacgtc atatcctaga tctttcagtg ttgattggac tagcaacgcc cctgctcctc
    15661 cgatgaatag tacttggcca aagaaccagc agacattttc catagctgaa gctatacctt
    15721 ttagttcttc ttcatgctta ggatggactt ctaacccttt tgattcgatg gtccccaatg
    15781 ccatcggtaa aatgattgga cgaacaaatc cagcaactcc accaaaactc acgttaaaag
    15841 ccgcaaagat cattcgaaag atcccataaa tatctacaat cataccagca gatactttct
    15901 tgaatcgctt gatcaaggaa gccgctgatt ctttcagacc atttctttca agcgttcctg
    15961 ttgccaacat aatgatgata aaaatcgcca tcccgcgatt gtttacaaaa cttgtaccta
    16021 aaagttctaa cattccttga atgccaagac cgccagtcaa agctgtaaca actaaagcaa
    16081 ccattacgat caatatcgag tctaatttaa atgcaaaccc gagaataaca atcaagacgc
    16141 ctaaaaggct aagataattt tccatatatt ttcctccata aaatgacaat atttttttaa
    16201 ttataaagca aaacacataa aaaatatagt tgttttctca cgaaatagac actattttta
    16261 aatatttttc gttatttttt tatcacaaaa attatcttac ttattgtaag gtaaagcttt
    16321 ttttaattta ttgcccgata atatatagaa ataaggtcta gttactgttt tttttaaatg
    16381 attaataaat aacaacttct ttctatgaaa ttgcaaagca ttcatcttct tttccgctaa
    16441 cgatatatcc gtcagtgctt ggtacacttt actaaaaagt atgagattgc cgatatctgg
    16501 ataggtgtgc tatactacag aaaaagatat acaatctgcc accgggtagg ataaagagga
    16561 tgatcattcc attaggtctt tgaagggatg attttcctct ttttgtgtat ttttttatag
    16621 ttaaggagga atgtaagtat ggcatggatg gaattaatca tagcaggaat tttggaagta
    16681 ttctggtcaa cgatgatgaa atggagtgat ggtttttcta aaatcaatta ttctttttac
    16741 acagtgattg gcatgattgc tagtttttac tttttgtcaa aggcaatcaa aagtttgccg
    16801 atgagtctct cttatcctat ctggacaggg attggcgcag ttggttcggt cataattggt
    16861 gtggtcttgt ttcatgataa gctgaatctt tccacttggt tctttgtggg gatgctactc
    16921 atcagtatca ttggcatcaa gattactagc ggccactaga cacttggaat aagtactgat
    16981 cagaaaaaat aagccttttt atttaatatt caattttttt atgaaaaaat aagaaataag
    17041 tccaatgacc tagcgacttc tccactcatc aggtgtatgc ttgtactatc tatagaaaag
    17101 gagtgaatat ttttgaaaag tctttcggtc caagaattat ccaagaactt tggtcacaaa
    17161 caagccctca gcgatgtcag ttttagctta tcccctggca aaatcgttgg attagtaggt
    17221 cctaatggcg ctggaaaaac aacgatcatg aaagctattt taggactgat ccattactca
    17281 gatggaacaa tctgtattgg tgaaaatgag atttctccct ctagtcataa agggttggaa
    17341 caagtaggag cattgatcga atacccgggt atctatccat tcttaacagg ctatgatcat
    17401 ctgaagttat tctctgaaac caacgatgta tctgctatcg acactatcgt gaatcagctc
    17461 aagatggaaa agtacattca caaaaaagcg aaatcctact cgttgggaat gaaacaaaaa
    17521 ttgggaattg ctcttgcctt actgaaccat ccgtctatcg ttattttaga tgagccgatg
    17581 aacggtttgg atccacaagc aacaagagat gtgcgaaaaa tcattaccga cttggctact
    17641 cagggcacct ctttcttgat ctccagtcac ttactcagcg agcttgaaaa aatggtcgat
    17701 gagctgatcc tgatcaatca aggaaagatc atcaaacaat gtacaatggc tgaattgaac
    17761 caaacagcgc aagatttcat cgttatctct acaacaaatg atctagctgc tcgaacgatc
    17821 ttgacagaag ctggatatac tttggaagat cctgataaaa tcaagctttt aaaagaaaac
    17881 gaagaattat taggaaccgt gatccagcta ttgtctgcac aaaatatcca tgtgacagac
    17941 gtccagcatc tgacgaatga tttggaatct tctctattgg aaattctgga ttcagtaaag
    18001 ggggaagaag aaaaatgaca ttgcttgtaa aacaagaatt atttaaattg atcaagaaaa
    18061 agtcaactgc tgttttatct gttctattag ttgttttgct cataggaaca gcacttctag
    18121 ccaaaaaata cacgacgatc attgatccag ttgaaatgac tgctcagctt tttagtgcca
    18181 catcttggat tgtctttatc atgattgcag ctgcaagcac gatcatttcg atggaagcac
    18241 aatatggtac attgaaaaat ctcctttatc gaaagtattc tcgaggagaa atattagtaa
    18301 gcaaatggat cacattagtt atttattctg tttatttata ccttttagct attattgtca
    18361 ctgtgttgat gaaactgatt cttttccctt cgatttcctt cacggagaag gtttctactg
    18421 gtcaaacact gatccaaagc ttgttcactt atactttggg tagttatatc ggtttatggc
    18481 tgattttaag tttagtgttg atgattgctt gctttatcaa tagttccggc gcatccatct
    18541 cagcaggaat cgtcttttat tttgcttcgt caatcatatc tggtatcctt attgcactca
    18601 tccaaaaatg ggagtggatc aaatggaatc cgatcagtat gttgaatctg caaaaccaaa
    18661 ttggaaacga agagatcatg aagcaattga ctcatctttc tactaatcaa atgctattcg
    18721 gtaatttagc ttacatcgtt ttgttcttag cattaggata tctcgtattt aagcgaaaaa
    18781 atgtatgatg actcatcaaa aaagaggctg tgaaagagac gttcagcctt attgccgatt
    18841 ggctgtctct taaacaccgt ttattctgaa aaaggagctg tgacaacatt tgtgtcacag
    18901 ctcctttcct agtatgtttt tgaataaacg acaggctcac aacctctctt tatacttctt
    18961 ttatacttct atacgtgaag catcccagtg gcaaataaaa ccattgcaat cactccgacc
    19021 atcgtcacac ctataagtaa aatcttatct aagtggatat gttgtacagg ctcgttttgc
    19081 tccttacgtg ctttgaggta aaaataaatg cctggcaaga aaagaacaag aacaagcatt
    19141 acttcattaa aggctacgac tagcatagcc catacttgaa aggcacttgc taaaagccct
    19201 actaaaaatt ggacccattc tttccgctga taagaaagtt tcatttggta caatcctaag
    19261 aaaagccacg tgaagaaaat agctgctgta ccaagtgtgt aagcaaagtt ataagcactg
    19321 tcagtgaata ataatgaaag taaaaagaca ttcgtacata acgtcgtcaa tacgatagca
    19381 taagttggtg ccccattttt gttgagtttt ccccatgctt taggcaaaag tccgtcttca
    19441 gccatgtttc ccattgtttc aatcggtaag atcgtccatg agagccaaac accaactaaa
    19501 gaaatgatca aaccgccgtt gatcacgact gctccccatt taccaaccat catttcaaac
    19561 acatagccca tagcaggctg cgccttgatc gtttgcagct gattcatcgt cattgtccca
    19621 tatggaagca tggaaaccaa gacgtaaata ataatcaagc cgaatatccc tagaatcgtt
    19681 gctttcccaa catctgattg tttttttgct cgtgaaccta cgacagaggc tccttcaata
    19741 ccaacaaata cccaaagcat caccatgatg caactcaata cttgactgga aaacgggatc
    19801 ggctgtccat tcgcattcaa tataatgtcg ctagaaaagt tatcaatgaa ttgttggatc
    19861 gaaaacgtgc tgatagctac tacaataaat aaaaataatg gcactaattt acagaatgtg
    19921 acgatagcat tgacgaaact agcattttcg actccccgat tcactagcca tgcatatccc
    19981 cataaaaaga ctgtactcat cacgatcgat gccagatttt gcccaccttc aaatactggg
    20041 aagaaatatc ctaatgcgct catcaacaat gttgcaaaag ctacgttgcc taaccagcat
    20101 gtgagccagt aaccccaacc gctgataaaa ccagcgaaac ggccaaaacc ttcttctgcg
    20161 taactaaaga tcccgccttt taaatctgga cgttttttat tgatattatt gatcgacaag
    20221 gagagcatca acattgctgt tcctacgagt acccaactga aaatagctgg gccgccagcc
    20281 gatccggaga tgtccgtcat aataccaaat acgccacctc caatcgctga accaattact
    20341 aaggcagcaa gtggaattaa accaattcct tttttctgtt gctctgtatc catgtaagca
    20401 tcctccaaaa taaaaccgct tttatcaaaa agtataacaa acctttttta tttttactta
    20461 attaataaaa actttaatag aaatataatt ctaccggctt ttttctcatt atacaagcaa
    20521 agcgcctagt ccctttccaa attacaccac caaaaaactt aaaaaagatg ctatactaaa
    20581 aggaaagaat tggaggatga tccatgaaca ttttatatat atcgatttca ggaaatacgc
    20641 gtgcctttgc caaacatcta gcagaatatg ctgaaaaaat gcatgcagaa gatcctgtca
    20701 atcctgaagt tactttaaaa gaaatccatg aaaatagtga ttttacgaaa gaaacagaac
    20761 cattttttac tttcgttcct acttatttag acggcggaaa tggtttagat aacggtgata
    20821 cagaaatctt gaccgaaacg atgcgtgaat atctggaaca tgaagacaat cataaacttt
    20881 gtcttggtgt agtcggtagc ggaaataaaa acttcaataa tcagtattgt ctaacagcca
    20941 aacaatatgc acaaacattt ggctttcctt tcttggcaga ttatgaatta cggggaacac
    21001 catctgatgt gacacgaatt tatcagattt tagcagaaaa caataaataa taaaaaagaa
    21061 gaagctgaaa aatagatttc agtttcttct ttcgtttcta ttgtttctat catattaagc
    21121 tttgacatcc atcacaggtg cttttgcttt tgctttacct gttttttcca agacaaagtt
    21181 ttttacacga tccatattgg ttaccaagac gatcatggat gtttctttgc ctgctgcttt
    21241 gatcgcttct aagtcagctt ctgctacaag cgtatcaggt gtcacttgct gcccttctgt
    21301 tactttcaca tcaaatggcg taccgcctaa gtcaactgtg ttgattccca tatgaagcaa
    21361 gatttctaaa ccatttggcg ttgtgatccc aatagcgtgt ttcgttggga agacagagct
    21421 gactacccca gtaactggag aatagatatg tccgttttct ggttctacaa agtagccttc
    21481 acccatcatc ttttgtgcaa aaactggatc acttgcttta tctaaagaaa caactgttcc
    21541 gtcagcaact gatgagagga tagtcacact ttcagctgaa gtttgaggta ctactttttc
    21601 aggcacttca tttactgtgg ctgtttcagt cgctaattct tctttttcta aacggtcttt
    21661 aggaataccg aagaagaatg tagcaacgaa gccaccagca taagctgcca ataatccaag
    21721 gatgtagcct aaccatttac cgtcagagat caatgggatc aaagcggctc ctgaaggtcc
    21781 aatagcgata gccccgacat ttccgatcat tccgatcact gccccgccga tacctccacc
    21841 gatacaagca gtaataaatg ggcgtcctag aggtaatgtt actccgtaaa tcaatggttc
    21901 gccgattcct aggatcccga ctggtaaagc tcctttgatc atttctgtta attctttatc
    21961 ttttttgcag cgaatccata atgccaatgc tgcacccact tgtcctgcac cagccatcgc
    22021 aagaattgga agcaatagcg tacgaccagt ttcatcaatc atttgcacat ggattggagt
    22081 caaaatttga tgcagtccga acataaccat cggcaagaat aatgtgccta gaacgaagcc
    22141 ggcaaatgct ccaccaacgc caagtaccca gttgatccca ccgaccatgc catcagaaat
    22201 gaaacctgct aaaggcatga tgaagaaaat ttcaatcaga ccgatggcaa tcaatgaaag
    22261 agttggtgta acgatgatat ctaccgcatc aggtactact ttatgcagtt ttttctcaac
    22321 gattgacaat agccaaacag cgaagatgac acctaggatc ccaccttgtc cagctgcaag
    22381 agcttcacca gtaaataagt ttttcagtgg tgcttccgga ttcatccctg tcagcatcac
    22441 aacggccccg ataactccac caagagttgg tgttgcacca aaaacttgtg cagcattgat
    22501 acctgtatag ataactaggt agctaaacat cccgtttttc aagatgttca tcacatcgat
    22561 atattgttgc catgttgctg catcaagtgt tccggctgtg actaagtttg ctaaaactgc
    22621 ggcaatccct gccacgatcc cagtaccgac aaatgcagga atcattggca caaagatatt
    22681 agaaatgtct tttaataccg atttgatttt agatggtttt tgtttcgatt tttgtgccgc
    22741 tttcatttct tgtgcccgtt cgtttacacg gtcttttcca gatgctgatg aatttgctgt
    22801 agctgtactt acagaagaaa gtttttctcc taatttcacg cctgccaaat caaccatttg
    22861 ctgtgctact ttgttcactt tcccaggtcc gatgatgact tgaagctgtt catcttctac
    22921 tacaccaagc actcctggaa tcgcttttaa ttcagagaca tttacaagat catcattgat
    22981 gatcttcata cgcacacgag tcatgcagtg gacaatgctt tctacattgc ccatgccgcc
    23041 tgccccttta taaatttgtt ttgccagttc ttccaaagtc agttcgtttt ttgccatggt
    23101 cttttccccc tcataaggtc ccttttacaa atccgtttgc ttcagctaat tttgcttgag
    23161 cttcctcttt atcactattt gtcaaaatca tgacgatagc cagcttaaca ttttgatctg
    23221 cttcttcaaa gtacttttca gctgtttcgt aatcagcttc tgtcgcttgc atgatgattc
    23281 gttttgagcg ttcgattagt ttttcatttg ttggtttgac atcgaccatc aagttattga
    23341 atactttacc tataccaatc atactgatag tagaaatcat attcaagata agtttttgtg
    23401 ctgttcctga cttcaatcga gtagagcctg tcaaaaattc tggacctgca tcgacttcga
    23461 ttgggaaatc cgcgtgttta ctgatttcgg cttgtttatt gcaagaaatc gttcctgtga
    23521 cagcaccgat cgattttgcg taatctaatc caccgatcac atatggtgta cgaccgctag
    23581 ctgcgatacc aataactata tcattttttg tcaaattcag cttgattaga tcttcttgcc
    23641 ctagctcata agaatcttcg gccccttcga cagctacagt cattgcttct tctccaccag
    23701 caattagacc tatgaccatt tccggttcta caccgaatgt cggtacgcat tcagcagcat
    23761 ctaacacacc tagtcttcca cttgttccgg ctcccatata aatcagacgt ccaccttttt
    23821 taaaggactc aatcgtgtta gcaatcactg gttcgatact tgtcagttct ttttcaaccg
    23881 cagcggccac tttctgatct tctttgttca ttagttgaac ggcttgagaa atactcatct
    23941 catctaatcc aaaagtttct tggtttctgc gttccgttgt taggttctct aaattgattt
    24001 tgctcatttt tatcttcctt cttttcttat aaattgatag ttcataccag ctttgatttg
    24061 gttgatcaat ggtagctctt ctttgataac tttagctact acattcactt tttcatccgc
    24121 tggcaaatca tatttggtta attggatctc acccatatag cgcaaatact tttcgttatc
    24181 taaagtaata gaccctttca tacgcgtagc tgtatttctt gctgggatag gcggaatctc
    24241 tcgaaaacgt gcatctacgc ttcgtatgac atctcttgca tcgtcttgcc gattcgtatg
    24301 cttccctaat atatattcgt aaaattcttc atctacaggt tccgtatgga gcaagactgt
    24361 ctgttctttt tggaaagaag caaattgctc ctgaacgttt tctgacagac ctgcatctcc
    24421 gatgtaaaca atatccgtgt tacttgctaa tagatctaga gcgcctgcaa gaggatggat
    24481 ccctcgatgt tcttccaaag ttggcaatcc ttgatatagt gggcctctca actctgtatc
    24541 tcctggtaca aaaccttgga caaggagacc ttgaattttg agccattgat tttttttctg
    24601 ataccattct ttgtccagcc ctgtttcggg acgtggataa taattatgcc aagcttcaat
    24661 ctgtgagaaa tcagcttctg cttctttcaa ttcatcgata tctttaggcg tgatcgtgct
    24721 agcattcaaa ctgattttca attgatgtga ccagtcggca atctgctgat tggaaatatg
    24781 ataatccatt cttaaacctg ttacaccaat tgctttcaaa ggcgttagat cttctaatga
    24841 aaaacccgct cgttccaatg cctctcctga aatatcgacc atcaattcta gtttattctt
    24901 tttcgcttcg cttcctagtt cgatcaatcg cttttgatat gccgaaacat catcttctgg
    24961 tatatgcatg gaagtgaaaa taccgacaaa gccattggat gccatttggc ggatatactg
    25021 tttcttctct tctgacaaag aatcgttcat aaatattgaa aagccaaaca tattatccct
    25081 ctttttctgt atccgtcttc atttctatga ctagagtata tatctaaaga aacaaaattt
    25141 caatactttt attaaaataa ttaaatttca tttctaaagg ttgagtttat attatcaaaa
    25201 gaattgtcat aacctaaaaa aggcactgct aagcaaaagc agtgccttga aatattttat
    25261 ttaaataaca caaaaacata attactcata agaaaccaat tctgtattat ctaccatgaa
    25321 tgtaaagaac ccctcattag gcatcggatg aaaataagca ttgatcactc ttatcaaggc
    25381 attatggcaa actaacagat agacattttc ttcgtcttcg atgcattctt tcaataaagg
    25441 aacgatccgt gcatacacat ctaagactga ctccccattt gggaatcgta cagcgaacgc
    25501 taaacgtgct ttttgaaagt tctcatcttt gcttggcagt ccatcataat ccccaaaatc
    25561 catttctacc aatcgttcat caacggtcag cggcaagctg agacgatcag caactgcttg
    25621 tgctgtatct cttgctcgtt gaagagggga atggatgatt ttcgtgattg gaacaggcaa
    25681 atctactacc ttttctgcta gtttctgtgc ctgcatctgt cctttttcag tcagcggcac
    25741 atctgcccgt ccgcaaacta atccttgtgc gttccaagtt gtttctccgt gtctcgttac
    25801 ataaagcatg tgattctctc cttcgttggc aaagttcccc ttaaccatac cttgttgttc
    25861 ttgagattac aagggttaga ttctctcaag agaaataatc tcagtagata ctttatccag
    25921 gaaggtttga tcatgttcca ctagtaacat ggcaggtttc acgttcaaga tcagttgttc
    25981 taattgttct tgattgaaga catccaaata attcaatggt tcatcccatg tatatagttc
    26041 agctggctgt gataaagatt tagccaattc cacttttttc cgttggccca tactcatctg
    26101 ctcgatcttg ttatgaaaaa catctctttc catcccaagc tttcggaggt tgttcaaaaa
    26161 tgcttggtag tctacttggt ttttctctgc aaattccgcc aacgttcctc gattgtcttc
    26221 ataattttgg cttgcataac taatgctcag atgttttggc tggtattttt ctcctataac
    26281 cttgccgttg aatgccccca gaagataatg gataatggat gactttcctg caccgttagg
    26341 acctgaaatc gccacccgct gatgaggctc gattgtaaaa tgaattggct cgaataacag
    26401 attttcatac cctaattgaa gatcttctac gcttaaaagt cgcttatggt gagacgcttg
    26461 gctattcatc gtcaaagaat cgatatattc gatatctttt aatagttttt ctttctcgct
    26521 gatctgggtc tccatccgat tcacgattgc tttggatcgt ttcatcgtcc gtgcagcatc
    26581 agcacccact gctcctttat tcactcgtct agattcagta tcgatgaatc cgacttgttt
    26641 ctttgtttta tctccttctc gggaacgaga ccattcggct ttttcagctg ctgttttctt
    26701 tagcctactg acttcttttt tcaatttttc attttgagcc atttcaaatt catcacgaag
    26761 ttttttctgt tcttcataga tagagaaatt cccttgataa agttccagtt gacttttttc
    26821 gattgctaaa acatggtcca ctacttcatc gataaatccc cggtcatggc tgaccacgat
    26881 gaagccttgt tttttctttt tcaaataagc cgctacttgt tttctaccag agatatccaa
    26941 atgattcgtt ggttcatcga ttaacgggaa atgagtgtca tccacaaata aaagtgccag
    27001 taggactttt gtcttctctc cccccgatag tgtgctgaat tctctccata agatttcagg
    27061 atctgtttgc atcaattgga gctctctttc gatttcccat atctcaaaat ccgtaatatc
    27121 atttaacacg taataggtta aacgttcttt atcttttgtc tgttgcggga aataggcaaa
    27181 ttcttgctga tggattactt gcccttgata aggtagtttg ttttgtagaa tattcagtaa
    27241 agttgtcttt cctcgaccgt ttcgtccgat aagtcctagt ttccattgtg tgtcaaaatt
    27301 tagatttgct tgttcaaata ataatgtgcc ttggctgtcg tagccgaatg tcagattttt
    27361 tatttcgatt ttagacattt tccatcacct cgtaaaaaaa gaccagtcct attttcagaa
    27421 ctggctaaag aggatgcaag ggttggatac acacaaaaag acccatacat cgaaaaaacc
    27481 aagccaatcc cgaaaatctg cacactgctt ccttgtctcc acaaggtaca gtctgaatgc
    27541 agctttcatc aatcgactta atttttcaat gcacggatcc ctcactttgt cctatatttg
    27601 aaattacgat agcataaaga aaatgatttt gtcaactact tcgggaggat ggtgaaaaat
    27661 ctgctaagtg tctgtcctaa gatttttgtt ttttctaatg tttgcctgtg ttccattgct
    27721 tcttctaaac ctattggacc caaatgaata cttaagacaa ttgggagaaa atcagaaagt
    27781 ccttctagct cttggtctct acttccgcaa atagcgatta ttggttttcg atattttttt
    27841 gcaagcactg ctacaccgta tggaactttt ccgtggatcg tttgctgatc gatccgccct
    27901 tctcctgtaa tgatgacatc tgcttgttcg atcttttttt ccaatccagt gatttggctg
    27961 actaaatcga aaccagaaac catcttcgct cccaacaaaa aaagggctcc accaataccg
    28021 cctgctgctc cacttcctgg aatatcatct aaatccacct cccagaaatc ttttatttgc
    28081 tgagcaatct ttcctgcttg tttatctaag aaagctagcg tcgtatctat tcctcctttt
    28141 tgtcttccaa attgccaagc agctccttgt tgacctgtat atggattggt aacatctgca
    28201 gcgataatca attttacttt agaaacaaga acgtttcttc gatcaaaatg aagtttttta
    28261 gtagataata aaggatttcc ttctgtgtac ccttctaaag aagcacccaa ggattggagt
    28321 aatcctagac caccatcgct acacccgctt cctcctaaag tgacaataat ttgttctact
    28381 tcttgaagca gtgcatcttt aataagttct cctaaggcat aactggaagc aagtcggata
    28441 gtttgatccg ttggtttatc aagtagatct aaccccaatg cttgtgcaga ttcgatgact
    28501 gcggtgtttt ttccttgcca agttgtcaat aaatagctga tttttcgctg acggaataag
    28561 agatccatcc catcaatggt acgccatgtt cccctgagcg cgtaatagat tgcttctgca
    28621 gtattttctc cgccatctgc aatcggcaca ttgatttttc ttcctttacg atcttcccag
    28681 ccatttagaa ctgcctgatt tagttcaaat gaagaagcac ttcctttaaa tgaatcaatt
    28741 gccgccaaaa tatccatctg tatccctcac tttcttctta agtaagacac tttttttcat
    28801 ttttttcaaa caaaacaatg ctattttctt ccatttttct atatactaaa agggaactca
    28861 ttgaataaag gagagaaaaa acatgaaaat cgcaatagct ggggccggag caatgggcag
    28921 ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc ctcttgatcg atcgttggcc
    28981 tgctcacatt gaagccattc gtacaaatgg gttgattgct gactttaacg gtaaagaagt
    29041 ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc gaaagcaacg aacacgtgga
    29101 cttgatcgta gcattaacaa aagccaatca attagatgat atgttttgtt cgatccaatc
    29161 cattattact gacaatactt atgttttgtg tttactaaat ggattaggtc atgaagatgt
    29221 actggaaaaa tatgtgccaa agaaaaatat cttatttggg atcacgatgt ggacagctgg
    29281 tctagctggt cccggaaaag tcacattgtt aggtgatgga gaaatcgaac tagagaactt
    29341 ggaaccagaa ggcgaagcat ttacaaaaaa agttgtagaa gtctttcaag aagcaaattt
    29401 aaatccgatc tatagccata acgttcgtta ttccatttgg cgaaaagctt gtgtaaatgg
    29461 aacattgaat ggtttatgca cgattttgga ttgtaatatt gctgaactag gcgcgcaaaa
    29521 agcggcagag agcatggttc gaacaatcgt atctgaattt gcttctatcg cagcaaaaga
    29581 aggaatcata ctagatcaag aagaagtgta ccaacatatt gcttctactt atgatcctga
    29641 caacatcggg ttgcattacc catctatgta ccaagacctg atcaaaaacc atcgtttgac
    29701 tgaaatcgac tacatcaatg gtgccatttg gcgaaaagga caaaaatatg atattgctac
    29761 accatattgt gcctttttga cccaattagt ccatgcaaaa gaaggaatcc ttggcgcaga
    29821 ataaaaggct ctctgatgag aatcagctga atcagcagct ggttctcttt ttttaagccc
    29881 aataaaagat caaaaagaaa acacaactct catgatgcca taagaattgt gcttgtttgt
    29941 ttcagtacat aatctcttca aaatgtaggt tttttatttg ttttcaacag aggagaggtc
    30001 atattcgtat tgctcataaa tagtggaatc gttagtgata aatcgttttg cccaattttc
    30061 tgcatcataa aattccaata gctctgatag gattggctta aatggtgtat gcagaaattc
    30121 cggtaattca tctaaatcac gttctcgaag caaaaaaata ttttcaggac gataaaaatg
    30181 ataatgagca atcgcttgat tatccgtcac tagtttcttt tgaaaataca tggattcaaa
    30241 tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt tccagaatcc cgcgcgattc
    30301 ccctgttttt ttcaggtact cttcataagt aagatatcca ggttgttcat tccctcgatg
    30361 tggaagaatg aaatagttgg tcgtaacatc tctttttata aattcctgct tatagctttc
    30421 tgctcgttct tttctcccat tattcgttgc accaaaaaat agatccgtct ttggttcgct
    30481 agaagaaaaa ttcatatgtt tcgaataaaa tgaactatta tgtttcagcc caagttcttt
    30541 tgcttccaaa aaatcaaaat gataaatctc atcgattgcc ggttcatctt gtgcctgctt
    30601 cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa aataacacaa tttttgcatt
    30661 ggtaaatggg cgcacgcgtc gagccatctc catatcaaat ccataatcta aaaaaacgat
    30721 cagctgataa tttgccaact gttgtctagc tgatttaatg aattccgact gtagtaaatc
    30781 ttttccggac caaacatcta aagagcggat ggaaggcaaa aaataaagtg atttattttt
    30841 tgcaactagc agtacgtcat ttttgttcat cttttccgcc tcactttctc tgtcactttt
    30901 tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa cgtttcctat atttgtatga
    30961 aaatttaaca tttgtatgaa aatatgatga aataaatcat tcttctatta aaattcgcct
    31021 aaaagttttc tgaatagttg taattgtctg aaaatttgtt atcattagtc attgttaaat
    31081 attgctattt attcttaata taaattttga ttgtgaattt aaaagaaaga ggtcatttat
    31141 ggaagataaa aaattgaaaa aacaacttag ctccagacat atcaccatgt tagctttagg
    31201 tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga tcgccggccc
    31261 ttctgtattg attgcttttt tgattggtgg gctgatttta tatatcgtga tgaaaggtct
    31321 gggaaaaatc gttttaagcg gtggcgacac gcatcacgga ttatctggtt tgatacgccc
    31381 ttatctaggt gctcattctg ctgattttac tgattgggtc tactggtcta tgtggattat
    31441 caatatcatc gctgaagctg ttgcagctgc ttcctttttg cagttgtggt tcccgcacat
    31501 tccagcctgg gtatttgttt ttatgttggc tgttttaact acgatcatca atctttattc
    31561 ggtacggtta ttcgcagaaa cagaatattg gctggctttt gctaaaatca gtgtcattat
    31621 cttactgatc atttttgcta cttatcttgt cggtcagcaa atgttgggca caggcgtttt
    31681 ccctaccctt cagcaattga ctgaccatgg tgggtttact cctcatggta tgaagggaat
    31741 catcagttct ttgcttgtag ttatctattc ttacggcggt tctgaactga ttgctatcac
    31801 ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag gtgtcatggg
    31861 aagaatcatt tccttttaca tcattccttt gtttttgctt ttgattcttt ttccttggaa
    31921 tactttagca ggcacgaatg tcagtccttt tgtgatggta tttgaaaaaa tgaatatccc
    31981 atttgcagcg gatatcgtca attttgtgat tgtccttgct ttgttctcat ctatcaattc
    32041 gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa aaggttcctc
    32101 tagaaaacta gctgttttaa acaagcatca agtaccgcaa cgtgctgttt tattttgtac
    32161 cagtgttctt tatcttggtg tagctctttc ttattttgtc ggtgacaaat tatttggcta
    32221 tctggcaggt tccctttcgt atacagtatt attgatctgg atcattatca gtgcggcatc
    32281 ctttgtactg gcactaagaa aaggaacatt atgggataaa ggaatcagct tttttgcatt
    32341 agctgtctta ggattgattt tccttggtat cctgttcacg aactccattg gggtaactgt
    32401 attgactggc ctgctttacc tgttcattta tttcagttat caaaaaaaga atgatgcgtt
    32461 cgtattgacg aatgaatgaa atcgatagaa aattttattt tatttatgtg gaatagatgg
    32521 agctattgaa gaaaggagtt gtgacaaaag tcttgttgca actccttttt ccgaataaac
    32581 agtctcatga cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt
    32641 tctccatgct catgacatga tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga
    32701 aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg tttggtatga
    32761 tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag tcgtacacga gtattcatgg
    32821 cgttgatgat gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg
    32881 aaaacaaaaa gataaatgtt atgatcttag ccgctagtct tcttgttttt gccggttcct
    32941 tgtttatggt acgaagccaa actactgtca acgacacagc ttggatgaag gccatgattc
    33001 ctcatcattc tatcgctatc ctggcaagta aacgagctga tttaaaagat cctgaagtaa
    33061 aaaaattagc ggaagaaatc attcaggcac aagaagaaga gatcgacaaa atgaaaaaaa
    33121 tgatcaaaga aatggaatga ccattatccg tccaaataaa aaaagaggat gttccagaag
    33181 ttatcagctt caagaaataa gaagaagcta cttctggaac gccgtgaata aggggttgtg
    33241 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcctatttt
    33301 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt
    33361 gtaaaatcag cgttctgacc tgagtattag agagacaaat ggaaaaatag ttcctcatat
    33421 tttgccattt tttgagctaa taccgagggt cagctgattg aacaccgttt atcgagaggt
    33481 tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt tgtgaaatgg ctttccacat
    33541 ttttgtataa atttggctta ataccgagaa actggctttt gaataccgtt tattcgtaaa
    33601 agggattgtg acaaaagtct tgtcacaatc cctttttatg tttttattgt tctaattgcg
    33661 ctgtcttgct tactgcttct tgcggtttca caaatttttt tgagattccc caacctgtga
    33721 aggcactgac catcgaaaac acaggagaaa gcaaactgaa aaatacgaat ggcaagtaag
    33781 tgagtgtagg aataccaaat gtacttgcta cgaaacttcc ggcaattccc caaggcacta
    33841 agtagttgat tactgttcca ccatcttcta gcgttctacc taatacagtt ggatcaaatc
    33901 ctgctttttc ataagtttct ttaaatgcat taccaggcag aataacagat aggaattgtt
    33961 caccaataaa aatatttact ccgatacttg tcatcaatgt tgtaaaaatc aaatttggtg
    34021 tgttcgtcat ctttcttgat actcgttcca tcacggttga gatcaagcca aattcgatca
    34081 acaatccacc taatgacaaa gttagaataa tcaatgacac agtaggcatc atactttcga
    34141 ttcctccgcg gctaagcagt gaatcgattt gttggtttcc agtatttgaa acaaaccctg
    34201 attcgatgac cccagccaac ttcgttaagg aagtttgtgg gttttgaata aaaatcattc
    34261 ctactgctac agcgatattc aacaaaatcg ttaaaattgc cggcaccttt ttccatgcac
    34321 acagtaacat taaaaataaa ggaatcagtg accagattga aatagtaaag tttgcttcta
    34381 gaacatcagt cacttcacga atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa
    34441 tcatgaataa aaagaaagaa acaacaaatg ccgggacagt tgaccacatc atatgcttga
    34501 tgtgtttgaa caaatcggca tcaacaatag ctgctgctag atttgttgat tcggataatg
    34561 gagacatctt atctccaaaa acggcacctg aaacaatagc tccaacaacc agcgctggat
    34621 ttaagcctag tgttgtccca ataccgaaaa atgcaatccc aatcgttgac ataacagtaa
    34681 aggcactccc tacagcgcta cctacgattg cacacacaac aaatacagaa gggacaaacc
    34741 actggacact gatcaatttg aacccaatga ccattagagt tggaatgatc cctgcttgta
    34801 tccatacagc aatcaatgaa ccgactaaaa taaaaataaa gattgggatg attcctggtt
    34861 tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg gtatcttttt atcactgcat
    34921 acaacatgac cattccgatc gctgctaaga tcgtgatatt aggtgagacc tttaatccga
    34981 caacgccaat cgcaatgcct aaaacaataa ccattaatac aagtgttgct tctaaaaaac
    35041 ttggtgttcc tttttttccc atgataatct ctcctttttt agttgttctt atgttctaaa
    35101 aaagttaaga tatcggatta ctccgccgca attcatccgc gattcctcct ttataaaaat
    35161 aaaaagacct gttttaaact aaggacgaat gactccgcgg taccacctta attaaaaaca
    35221 gatctgtttt tcactttcaa acttttattc aaatgtatgg actttccatg taattcgtca
    35281 attaggcttg atcggttcac agcgaccacc gactttctga acaaaacacc taaccgctac
    35341 tgcgtgaaaa gttgacttgt acgaagttta ccttccataa gatcatttgt caatgaaaaa
    35401 gaaaatattt tttcattttt ctgaaaatta tttttataac tttcacacac aagtttattt
    35461 tttactagaa agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgct
    35521 tgattctttc taatagcttt ggctctttca acagatcaag agctgtattg gcgagtagtt
    35581 ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt agcagcagct ttgaattctt
    35641 ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt aggctggatt gttggtatga
    35701 cttggctgac atttcctaca tcagaagaac cgagacttgt tctttgttcc gtttctattt
    35761 cttcatctgg aacacctgct tcttttgcat gctggaagaa taagtcatca aaagaaggag
    35821 taacgatgac atcatcaact gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac
    35881 ttatcgcagc acctcgtacg atattttcca ctttttcata tactttatct aatgtttgcc
    35941 gatttcctgc acgtaaatag aaacgtccag cagcgtaatc tggaatgacg ttggcagcta
    36001 ctcctccgtc tgtaatgatc ccatggatat tgacatcttt tggcaattgc aagcgtagag
    36061 cgttgattcc attaaacact tggatcaatg cttccaaagc atttactcct ttttcaggtg
    36121 ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc gactggatca ttcgctaaag
    36181 attctgtcgt tttcccataa cgaaaagctg gatggacgca taacgctaca tcgacatcct
    36241 caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc gttctcgccc ccttcttccc
    36301 ctggggtgcc atatacacgg atttctccac cagtttcatc aatgaattgc ttcactatgc
    36361 tggcggctaa tacggagtaa gtaccaaaca aattatgccc gcatgcatga ccaatacctg
    36421 gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg gaaatataac
    36481 gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc tacccaaaac ctttcttttt
    36541 ctaattgttt aatcaacgct gctgaggaat aaaactcata gttacttact tcaggatgtt
    36601 cgtggatatc tagagcaagt gcttgatatt ctggtaaacg atattggatt agatccttca
    36661 attggtttgt tgagattgtt gttgtcataa aaaattcctc ctatgctagt catttttctt
    36721 attttctccc gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt ttctcgatta
    36781 ctttgactgt gtcttctgtt tgataagcgt ctactatttt ttgatacgtt ttattgtttt
    36841 catcttcttt tcttgcaacg atgatattca cataaggacg tgcatggtca tccactggtt
    36901 ctaagaagat ggcatcagaa gcaggattaa atccagcatc tacagctaca ccgctattga
    36961 tcactgaaat gtctacatct gacaaagcac gtgctgtttg ggaagcatct aattcagaaa
    37021 tgtctagatt ttttctattc tcggtaatat cagaaacagt gggcgcttgt ccttttgcct
    37081 tatccacttt gatcaatcca gcagtttgca ataacagaag agctctgccg ccatttgtaa
    37141 catcattagg gattgctact tttgctccgt ctttgatatc agctacatcc ttcaaagtgt
    37201 tagaataaat tcccagcggt gcattgacag tatttccgat cgatactaaa tcagttccgt
    37261 gttctttatt gtaattgtct aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc
    37321 catctgctaa agccacattt ggttgattgt aatctgtaaa tttcacgtat tccaaattaa
    37381 tgttctcctt tttcaaacgg tctttcacgt tgtcccatac atctgtatct tctccaataa
    37441 tccctagttt tacgatagtt tcttttttgt cagttgtatc tgcttttcct tggctacacc
    37501 ctgccaacca aataattgct acaaatgtaa tgactacaaa acgaataatt tttttcattt
    37561 tttctcctcc actttcaatg tgtaattttt ttaatcagtt gattactgat aaattgactt
    37621 aaaaaaacta agattagaat caataatgta gccacaatcg ttacatctgt ttggaatcga
    37681 ttataccctc tcgatatagc aagattccct aatccgcctg cacctactgc tccagccatg
    37741 gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga cccgaatgat accaggcaaa
    37801 ccttctaaca aatacacacg taaaatgata ccgattggac tggttcccat cgcttctgcc
    37861 gcttcgatca ccccaggatc aacttctagc agtgcgttct cgatttgacg agcaaaaaac
    37921 ggaacaactc caaaaatcaa tggcactaaa gctgctttcg caccaatcgt cgttccaact
    37981 aagaaacgag tagtcaatgc taaaagagaa agcaagataa tgaaagggat ggaacgaatg
    38041 atattgatca gcttatcaag tatttcaaat agcagacggt tagctagtaa tccattaggg
    38101 cgtgtcacta ctaacgtcac acctaagatg attccaatca tcccgccaat gaatgctgtc
    38161 caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa
    38221 acattgggca aatatgtttt aaagaaatca gtcattttat cttcctcctt cgatcacttt
    38281 gatacgacta tctaaatcgt ttttttgtaa aatatttatt tttacaccag atttgatcaa
    38341 ataatccaat gcttttttcc gctgtttggt ttctccagct aaagtaacga tcaaactccc
    38401 taacggcgtt tcttgtaata actctacatt gccatataaa atattcgctg ttacttgata
    38461 tttgctgtac agatgagcga ttagaggttc gttcgtctga tttccaatat aacttaattc
    38521 caccagccat ttattggcga tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa
    38581 tgcttggtca agatgagtag ctgttcgaat aaaatctttg gtcaattctt cttcaggatt
    38641 gctgaagatc tggatgctgc tacctttttc aacgattctt ccatcttcca taaccgctac
    38701 cttgttgcag atttctttga caacttgcat ctcatgagtg atcaggacga ttgttaaacc
    38761 taactggcga ttcaattttt ttaacaatgc aaggatctga agggtcgttt tcggatcaag
    38821 tgcactggtt gcttcatcac ataataatac cttaggttca ttcgccaaag ctcgtgcgat
    38881 tgctactcgt tgtttttgtc cccccgacag ttgtttagga taagcagatg ctttttcttc
    38941 caaaccgaca agttccagta gttcattcac tttttgacga cgttcctgtt tcgattttcc
    39001 agaatacttt aaagaaaaat caacgttgtc aaaaatagtt cggctttcca ttagattgaa
    39061 atgctgaaag atcataccga tggcctttct ttcttttcgc aattctttgg gagagagctg
    39121 actcagattc gtctgattga caatgacttc tcctgctgtt ggtttctgca gcaaattcat
    39181 cacacgaact aacgtacttt tccctgcacc tgagtaaccg acgattccat aaacatcgcc
    39241 tttttcaatc gttaaatcta cagaatctac tgcctgtatc tctttttctt tttgtttgaa
    39301 acatacagaa acatctcgca attcaatcat taaaattcct ccttatctaa aaaaagcgtc
    39361 ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta ccacctcatg
    39421 ttcaagctgt gttcacacac aactcctctt caagtacgat catactctag caagatatcg
    39481 gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg gccattttcc
    39541 aatagtcgtt ccttgcttct tctcagctac cgaagctctc tgtcaaaggt tcttctatcg
    39601 tacttaccat ttcaatgcaa ataatattca aaaaaaaagt ccttttacta acataaacta
    39661 tgtcagtaaa aggacgacgt atcgcggtac cacctttttt cggaagtttc cttccctcaa
    39721 gcccaacatc tcaaaaagag agattggcgt tcgttttaac ggtcgaacag ttccgaattt
    39781 gcctacttat cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc
    39841 tcaccagcca gagcctctct gcaaagtttc caaacttact ttccttttca acgaaatcga
    39901 tattttgttg ttgggattaa tctacatgag gaaaactgaa aagtcaagta cgaaattcaa
    39961 attatttttt ctatatttat ttttctgttt ttttatttaa catttcaata acagttcgaa
    40021 gatcttttcc ttccgcaaga agtttcaatt tttccttttc ttcgacggta taatttcgga
    40081 caaaactgga atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc
    40141 ctactaaaga atcttccact gcaaaggctg aactagcttc taatcctgtt cgttccaaag
    40201 ctgtcagata aggaatcggt gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg
    40261 tgtccaagta atcagctaat ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag
    40321 tggtcgtagc aatgcctagt ttcatatgac gttcttttaa gtctgccaat atttcttttg
    40381 catatggttt taagtggatg gctccgcttg tcagctgttg ataaaaatat tcttcacgct
    40441 tcgctcggac ttcctgtact ttttcagggc tacctgctgc tttcactaaa atatcaaaac
    40501 tttgtttcca gttttttccg ccccaagcgt caacgatttc ttttgggata tggatatcaa
    40561 attgctgaaa tccccaaagc cagccatcac gatacgtttt ttctgtgtca actaacaaac
    40621 catctaaatc aaatattgca cctttcatct atacattaat cctttccaaa acaatacaca
    40681 tagtttagca cgactggtcc aaaaagaaca gaagcaaaga accagccttc tcgtttataa
    40741 gagttcttgg aatcgacgga tatagatttt cttcacagct gtcacaagcg ccagataagc
    40801 gatgactgta gcaaataacc ataaccagta attgccagga agtggcatca agcctaattc
    40861 agcaccaaaa cttgtaaaag gaaggacaga accgacaata attcctaatg tagtgataac
    40921 aaacatggca aatgttgcgt tgctttggat aaatggtact ttcggtgtac gcagtgcatg
    40981 gagcactaag gtttgtgtcc atagtgattc gacaaaccat cctgcatgga ataatgcgat
    41041 gaaaatcact cgctgtgaac tatccaatgt atggaaactt ccgcccacta cagcaggaca
    41101 aataacaaaa tacatcaaca agtaagtagt aatatcaaat atcgaacttg ttggaccaaa
    41161 gtaaaccatg aattccccaa tactagatgc attccatttt tttggctcgt ccaaatattc
    41221 tttatccatc cgatcccatg gcaaggaaat gcaggaaaca tcatagatca gattcaaaaa
    41281 caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa tcagcacgga
    41341 aaacatattg ccaaaattag aacttgctgt agccttgata tatttcatga tattcccaaa
    41401 agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat
    41461 cacgtctgca gattctttcg caatatccac agctgtatct acagaaatac cgatatcagc
    41521 cactttcatt gcaggagcgt cattgatacc atctcctaaa aatccgacag tatgaccatt
    41581 tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta cgaatacttc
    41641 atggtcttca gctacttgtg ctaattcatg atcactcatt tccgagattt tctcaccggt
    41701 aaccaattca ttgatctcta gacctacttg acggcacact gaacgtgtaa ctaattcgtt
    41761 gtccccagtc agaactttta cagctacacc atgttctttc agagctttca acgcttgttt
    41821 cgttgtttct tttggtggat caaggaaagc aagataacca atcaaaacca tgtctgactc
    41881 atctttgact gaaaattctc caacgacgct tggattggtc ttttgtgcga caccaatcac
    41941 tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt acggcttctt ttcgttcttt
    42001 tgttaatggt gtaacagtac cgccaagatc aataaattta gaaatgttca gcatttcttc
    42061 aatcgcgcct tttgtgatca tttgtgtttt accagctgga tcagcaacca caacactcat
    42121 acgacgacgt tcaaaatcaa aaggaatttc atccactttt tgataattga tcttatctgt
    42181 atctaaagtt tctgttgctt catcaatgat agccacatct aacaaatttt tcagccctgt
    42241 ttgatagtag ctatttagat acgcatggcg caaaacacga tcatcttcat ttccttcaca
    42301 atccatatga tattcaagaa tgattttatc ttgtgtcaat gtccccgttt tatccgtaca
    42361 taacacatcg attgcaccaa agttctggat ggcattcaaa tttttgatca ctgttccttt
    42421 tttggccatc acacttgctc ctttgaccaa attcgtcgta acgatcattg gaagcatttc
    42481 aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc aatccccttt
    42541 tgtaagccca ttgatgacga taactgctgg agccatcaat gacataaatt tgatcagtag
    42601 catggatgtt ttatggatgc ctaattcaaa gcttgattga attggttttt cagatagcgt
    42661 ttctgctaca tgaccaaata gtgtttcatt tccaacagaa acaatgattc cttccgcact
    42721 tccactgatg acattacttc ccataaagac aagattttcg taacttgttt cgctttgctg
    42781 atcgtcttca catactttcg catgcttttc tactggatag ctttcccctg tcaatgctgc
    42841 ttgggaaatg aataaatcct ttgcggacaa taaacgcata tccgctggta tcatatcccc
    42901 tgctgaaagc cgaacaagat ctccacagac gatttcttcc ataggaatct ctgtgtactt
    42961 tccattacgt tttacagctg ccgttacttt cacaagtgat ttcagttttt cagccgcttg
    43021 attggaacgt acggactgaa tcaacgtcat tgtcccgctg acgataacca tagtgaagat
    43081 aatggctaca ccgaaaagat ctttatctcc aggagctgct aatacatatt ccgtgatgaa
    43141 tgaaataatt cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat
    43201 ttctacaatg aatggtgttt ttttgccata tgaaatcgca ttttcaccgt aattttctct
    43261 tgaacgttcg atttgttctt ctgtcaatcc atttgctgat gcatggaata gttcaaataa
    43321 ttcttgtttc gtttttccag caaaagctgc atagattttt tgtgtatctt tctttttttc
    43381 catctcaatc tctcctttgt gtccaaaaga cagctgttct cgctgctttt ggtctttagc
    43441 tattcatcgg atcgatagag aaaaaactga aaggggctga gaacagatag ataatagccc
    43501 tggcctttcc tctagatcct tgtttttcga atgactgtca tccatttgtt ctcatctcct
    43561 ttactaagta attttaattc atgacttcca atcatttttc gaatagttat ttttccaatt
    43621 agaccacctc ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc
    43681 caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag atctcatcgt
    43741 tgagttttga cactgtacaa cgtaaaaagc agatacctgc ttttagctac cttatgaaaa
    43801 gccttatctc gacaattcct gttctaccca ttggcgtctc tcgacgtttt tgggcagtag
    43861 cctatgtttg tataggagcc tcacctaaca aatcttattc ggttttcctt cgttaatata
    43921 acaactaaaa aagaggatgt caaatcaatt ttccatgttt ttcatacttt ttttaaaata
    43981 cacacgttat attcctgtct cacgcggttt tatttgtctc attttccacc tcccgctata
    44041 atagaaatat agtaggaaac gaggtgctgt tatggaaaga acacttgtta ttatcaagcc
    44101 agacggcgtg agacgccatt tagtcggaag catcatccaa cgttttgaag caaagggact
    44161 ggccattgct gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag aacattatca
    44221 gcacttgact gagcgcagtt tttttgatga actgatcgat tacatgactt ccggtcctgt
    44281 cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga tggttggagc
    44341 aacaaaagct gccgatgctg ttccaggtac gatccgagga gactacgcgc tgccggggac
    44401 tgaaaacatc attcacgctt ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt
    44461 tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta
    44521 aactgctggt ctagttgctg ggttcagcaa tggccagcag ttttttctct aaggacctcc
    44581 gttgattctt tcctaacata tattcgagca ctgtgaagaa ttcttggtag tctttcgtct
    44641 tttcttcccc taaagcagca gaaatcaatc tttgataata atctgccatc aatgtttcat
    44701 agatcactgt acttctttct ttatgtgcta agctccgttt catatcttca ttcacactac
    44761 gtgccttttt caaccgacca ttttctatat aaaacgaagt caggttcatt aaaaaagtaa
    44821 agagatactg ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt ttgattttca
    44881 aataattgtt ctcaatgaac cgatcactga atatccaagg aaaatcagct agaatagata
    44941 gttcaaattg accatagtct ggtatctgca tcaagtattc ttcaatgatt cggcattcat
    45001 aacgtaaaaa ttcaaattct gtctcagata cttctgtttt ttcgaccatg tgaagattca
    45061 aaaccaaata atacagatct tctgttttct taaaatgcct acggttattg ctgatctctt
    45121 tataaggatc aaagctgtct tcttgtaccc gatctagcat tcgtcttttt ttctttaact
    45181 ttattggttc atccagctgg tttcgcatcc attcatattc tgcaagtgtc atgttcattc
    45241 gctctaaaaa tgatctcaat gttacaaatg acattttttt cactgaattt gtctcaatcc
    45301 cgactagaga agtcttaaaa cagattcctt ccgctaattg ggactgtgtc aacttcttgt
    45361 tgactcgaag tttcttgatc aattcaccat catacataat agttctcctt tttaatttaa
    45421 atttttccac attcaataaa tagatacgtt aaaaaggaaa aatacacgaa aaaaataaaa
    45481 aaatgttttt ttcataaaaa ataccaacct tcaagcatta gaatgttgtc taactactga
    45541 aggttggttt gattgtttat tgattatttt tttgctgttc aacgtctgtt cttgttactg
    45601 gatataagtt ggttgttgtt ttattaccat tttttgaaaa gacacttgtc gaatgttcgc
    45661 ctttttgctt ttcgattgct tcattacgtt ccgcttcatt tttatagtca tattttttcg
    45721 gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa gagattttcc tgattcagct
    45781 tatctgaaag acttaacgca gtcttgactt tttcctgatc tttggagatt tcctcatcga
    45841 ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt attgtaagct tttcctccta
    45901 ggacggtgta tttgggtgtc acaaagttcc catttcgaaa agcaactact tgatcatgct
    45961 gagaagataa taagtctgta ccaaaatgaa gataatcttt atcatcgatt cccaatagat
    46021 gtagtaatgt cggcaaaaca tcgatttctc caccgtattc atgattgact gtccctttag
    46081 tataaccagg catatggatc atcaatggca ctcgttgcat ctgtgcgttg tcaaattcat
    46141 cccatgtatc tggatcttta ccaagagcgc ttgctaaatc tttattatct gtattcgaga
    46201 tcccaaaatg atccccgtaa atcacaaaga ttgaattttg gtaaatacca gattttttca
    46261 aatacgtaaa aaattgttcc aatgattgat ccagataatg tgccgttcga acataattat
    46321 ctactgtgct attgccagta ttcaaagatg gaaagtcgaa attcttgtca tccgtataat
    46381 aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa cggctgttgt agatgctcaa
    46441 gatattgggc agattctctg aataaatctt tatctaatat cccataacct aacgtttcgt
    46501 cggattcatc aaaataagat cgatcaaaga aattgtcata acctaagttt ttatatacat
    46561 gatctctatt ccaaaaacta gctacattcc catggaaaac agcgcttgta tagccttgct
    46621 tttgtccaag gatcgctggt gcggcttgaa agacattgtc tgacccaagt tctgtgaata
    46681 atgagccttg aggtaaacca aatgtgccag tttccaacat attttcagca tcacttgtct
    46741 ttccctgtcc tacttcatga aagaaattgt caaagctgat cgttgcttga ttctggaaaa
    46801 tactatttaa aaacggtgtg acttcttgtc cgtctacctt catattaatc aaaaactgct
    46861 ggaaactttc taaatggaga acgatcacat ttttcccttt agcgatccca aatgtttcgg
    46921 gatttggctc tgcataatgt ttctttgtat aatctaaaac ttcatcaatc ccattactgc
    46981 ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat tccgtcataa atagtatacg
    47041 catcgattcc tagatatttg acaatatatg aacggtcaaa tgttcgctgc agcaattgtg
    47101 gacggttggc ttcgctcagc gctagatttc ctgaaaaaat aagccctgaa agacaggtaa
    47161 cagcgatagc catcggtttg ccgacagggt tgcttttgat aggtattttc ttgaccttca
    47221 accaaaccag tagcccaatg aacacagcga tatccagcca gtagatgaca tcatggggct
    47281 tcatcaaaga aaaggagctt cctgacaatc cttgcgacac tttcgaaaag cctaatacag
    47341 atttgactgt aatgaaatct gtgaattccc gataaaaaat aatattcaag tacaacaaaa
    47401 gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc tggctttggc tttttaatat
    47461 acaaagaaag gccaaaaaac aataaggtag tcgcaattgg gttgatccac aagataaagt
    47521 actggatcgt accttccaca cctaatgaaa aatctgcata gtaggcaatg atcgtcttca
    47581 gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg attttggcac aattttttaa
    47641 gttgtgtcac gtatatcact tctctctatt cacatttttt cttattttgc tcctgaaaaa
    47701 tgaaaatttc ctaaaaaagt tgttatctta agattttttt attctttcta aagattattc
    47761 cgtccacttt tcacagcata aaaccgctgc gatgtaagcg attaaactaa tgaagagata
    47821 aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa gccagtttct cggtattcgt
    47881 acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc ttatttcttg atgcaggaca
    47941 gctttttcac aacttcttcc ttaaacggtg ttctcctgcc aactcctcgg ccacaagtca
    48001 caatatttat aaaacatgag aagctgtttt ttgaaattgt tccttgtggc tcagagttaa
    48061 acggcaggct caagacctct tgatacacgg tgttcaatca gctgaccctc ggtattagat
    48121 tatgtcagga cgctaatttt acaacctctt ggtaaacggt gttcaaaagc ttgcatctgc
    48181 ggtaataagc ccaaccaaac gaaaaatata aggagctatt ttttgaaatt gctccttgtg
    48241 gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgacct
    48301 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt gtttctctaa
    48361 tactcaggtc aaaacgctga tttcacaacc tctgtttttc attgtccata ggattgatat
    48421 tgtccttgtg atgactcatt cgtaataatt gaggcttgat tttcttcact tcccgttatc
    48481 gtttgtccac tgccagaatc tgaaggactt gttcctgtcg actcgttttt ttgagaagag
    48541 acagttgttt tcttgacttg ttcatttgtc tttttccatt ccgctaaacg atcggcgacc
    48601 acttttttgt ctactttttc agaataagcg gtgatcactt ttccattttt caaacgggct
    48661 actttgtcgc catcatgagt caaaagcaaa acggtcaaat gcttgtcttt aaattgatat
    48721 tgtctgagag aaatcgcact aagtacgtca tcgatattaa taaattgatt ttcttctttt
    48781 ccttttggca aataaatgtc caattcgcct ttttcgtaat tggcagttat tttatactcc
    48841 gcccaccaat aagttgagaa ttcttttttg aaattatcta ctgattcttt gcttgctacg
    48901 attttttctg tttctttctg agcaagatct gctgtcattt ttcctgaaaa tgctgcggat
    48961 aaaataagca gtaagaaaaa accgaataac caaaagagaa gaaacggtct ttttttcctc
    49021 atgctttcct ccactcctat ttaattttga ctattctatt atatacaaaa aatgaaatag
    49081 aaagacaaaa aagttgaaaa agatcgtaaa taattgaact tctttttcac aaaatggtaa
    49141 gaatggagaa tttcacgcat ttgctctcaa ctattctttc ttaagcctca aatccgtttt
    49201 tatctgatac gtctttgaac caatgacccg attttttgat tgttcttttt ccatcttgtg
    49261 ctaagtctac tgaaataaag ccatatcgat tcttatatgc attggtccac gaccagttat
    49321 ccatacatgt ccacatatga tagcctaaac aattggcacc ttcttgaatg gcttgatgga
    49381 catatttcag atgagattgg acaaattcga tccgataatc atcgtggatc atgccatctt
    49441 ctttgataaa tcgtgcttcg ttttctacac ccataccgtt ttcagaaata aagcagtcga
    49501 tatttccata atgatctcgt acattgatca aaatatcata gattccttgt tcatagatct
    49561 cccatccgcg ataaggattc attttacgac caggcatttc ataattgtcg aaataatcct
    49621 caggcattgg accgttttta gtatcgattg gtgtttcttt tgcttttacc cggcgtggct
    49681 ggtaataatt gatacctaaa agatctactg tattttcttt gataaccgtc aaatcctctg
    49741 catggatcac aggcatgaga tcaagttctt ttactatctc aatcaattca cttggaaact
    49801 cgccttttac agctggatca aggaatgaac gattgaagaa cgcatctgct atttgcgctg
    49861 ctttaacatc ttcagggttt gattcgtctc ttggatagct tggtgtcaga ttcaagatga
    49921 taccaataac accaccttgc tccatttcat ggtaagattt gatggctaac gctgaagcca
    49981 acgcttcatg atagccaact tggatcgctt ttttcatatc tgactcttct ggataatgcc
    50041 atcgatataa ataacctgct tcgactggaa cgattggttc attatgagta aaccattttt
    50101 ttactttcga accaaacaat tcaaaacaac ttttggcata tttttcatag gcttcaaccg
    50161 tttctcggtt caaccatccg cctttttctt gcagatacat cggcatatcg aaatgataca
    50221 gattcacaaa gggttcgata ccgttttcaa tcaactcatc aaaataacga tgatagaaac
    50281 gaacggcttc ttcgttgatt gttccagatt catcaggtat caaacggctc cattggatag
    50341 atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg tacatcctct tgatacttcg
    50401 tataaacttg cgaggttttt tcaggtccta cttgatcaaa gaacttttgc ggttcttttt
    50461 tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc tgaaatcctt ccttctgttt
    50521 gtgggccact tgctgcagaa ccccaccaaa aatttgctgg aaattgatat tttttcatca
    50581 aagcacctct ttttcttttt tagttcgttt tagttgattc catgtatgat attttccatc
    50641 acaatcactt tttcattttc ttcctcttct tttttgattc gttttaacag ctgttctgct
    50701 gctcgttccc cgattttatc tgttggtggc gtgatcgtga ttttcggaag gatactcaga
    50761 tcgtttttat ctaaataatc atagtgactg aacgcaatat ccttcccgat tctgacacct
    50821 tcttgattga aacattcgat ggcaccaatc gacatattgt aattgctgac aaaaatagca
    50881 gttgcccctt tttctaataa tgcttttgcc ccgtaatagc cgctttttct agaatagtct
    50941 cctgtaaaga cctctaattg gatcgatgga tcattttgga ccgcttcttt ggctcctgct
    51001 aaacgttctc ttgctacgta gtctgtttga gtggcagcaa taaatccgat ttttttatgt
    51061 ccttgttcac catgatgagc gatcactttt gctacacttt ctcgatcatt cactaatatt
    51121 gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga taactggaat actcattttt
    51181 gtgagcattt caaatccaga ccactcttct tcggataaga agacaatcag cccatcgatc
    51241 acatgcttca tcaggtattc aattttttgt tcaatttttt ctggctgatc ggcaaacccg
    51301 cttaataata agctataccc attttcttca gctactcttt cgatccctga gacaacttcc
    51361 gacccaaaac ggctggaaat cccgtttatc aaaagtccaa tagagaagct tcgattattc
    51421 ttcaaccctt tagcaatgat gttttcttta tagtccaacg ctgaaatcgc ttctgctatt
    51481 tttttcatgt tcttttcctt cagttgctga ccattcaagt aacgggaaac tgttcccact
    51541 gaaacaccag ccatcttagc cacatcacgg atcgatgcca tacattctca ctcctgccag
    51601 ttacatttga tttctctttc ttgttgttcg attatgatga ccgcattctt tgcatagtgt
    51661 tgcttgtcat caatatccat ataactaaag tcttcagtag agcaacttcc tctcactatt
    51721 aattatactt aaacaagtat ttgaaccggt tcatttttaa tatagcattt ttttaaaaga
    51781 ttgcaacact tttttaaaac gctttctgaa agtggcggat tttttgcctt tttaaaattt
    51841 tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt tttctcttcc atccgtttga
    51901 agttttacat tccaatggta taatttcttt atcctatcaa atgggggaag tattttgaag
    51961 aaattaatcg tactatttat tttgtctgcg ggacttttga cattgactgg ctgtgatttg
    52021 tttggcggct ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt aaaagcggaa
    52081 aaagcagata ccaccaacag tgtatatacg ctaaaagtac gtaaagatat ggatttatcc
    52141 gcgggacagt tgacatttac agataaaaaa atggaatgga cacgcacgta tcagacacaa
    52201 ggaactgatt cttctgaatc atctgaagaa aaaacagtgc tgacagatat caagatcact
    52261 accaaagatg acgaatatat tatttcagga aaagaaaata aaaaagaaac aacagtcata
    52321 tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg gcaacatcta cagtctatag
    52381 tgccgtctgt tggatcttct tttgtttctc cccttctttt ctgctatagt aaagaagcaa
    52441 gatgattgta aaggagacga aatggatgat cgatagtcct gattattgta catgtaataa
    52501 caacaaaaat atccatacag aatttaatga ccaaggaagc ttgagcatat gcgacaactg
    52561 tcacaaacta gtcaaagatt catttaaaac atttgaatct tatcctgttc aaaattatgt
    52621 aaaaagtaga agaagataaa caaatccaag caaaactcac tatggctgat ttttatgaac
    52681 ttcaaactca gcttcttcct ttgatcaagc ttcatcagta aaacaaacag tggatcattt
    52741 cagtttacaa atcaaagagt aaagatttat ttatttcaaa tatttgaata taaaataacc
    52801 aaacgataga aaggcttatt ctacatcacg tagtaatttt tctttcccgt cgtttggttt
    52861 tttattttta gcctaattct tgaccttttg catgctggtt atccgtaaaa tattttccag
    52921 caggctttcc ttccaattca gctaattttt cttggatctg acggatcacc ttttttgcat
    52981 cttctggatt ttctacaaaa tcgaatttat ccccatccac ttgtatttta ggacatacat
    53041 caaaatcttc ataccattga ttatagcggg tgttcaacgt ttcataatag gtatataacg
    53101 tctcatcgct ttctagctgt tcatagtcac gcccacgttt tttgatacgt tctagcatcg
    53161 tgtccaaaga aactttgata tggaccatca aatcaggccg tttttttgga gcaacatcat
    53221 ctaactcgtt tagcatcgta tctaataagc tatcgtactg ctgtacttcg atatccgtta
    53281 cccgacctaa atcagcgttt agatggaaca acaggctatc ttcatagatg gatcggtcta
    53341 gcacattgtc atcgttcact aaagcatttt tcattgctaa aaatcgcttg ttcaaaaaga
    53401 agatttgcaa taaaaaagcg tactgctcag gatttgaata aaagagcggc aatacttcgt
    53461 tatcttcgac attttcataa aatggtttag aatcaatctc ttttgcaagc atatccgtca
    53521 aactgctctt acctgcacca atcgttcctg aaatcaaaat cactgacata caacccttcc
    53581 tcttctataa gtactcgcct acgtaagttt caacaggcaa tgtttcaaaa tcatattcac
    53641 gtacagcttg gtctgatttt aaatacgtat ggagactttg tgtaatcatc aagttcgtag
    53701 gtaccgtctc ttcttgtaca atgatcatcg gttttttcaa catagttgcc acacctaatt
    53761 cgtaagccgt tcctggatcg attgtttgat gctcaaagtc aaggatagca acaacaaatt
    53821 ctgcatttgt cagttcttcc atatcttttt cgtatacttc ctgcgcccat ccagcgctga
    53881 atagttcata attgctttct tggtgatgac gtggagagta gaaacttgtg actgttttgt
    53941 tttcctccaa cgctttttcg atacgactta ctcgatcgat ttgctcctct gaaaaaaatg
    54001 gtcctgctaa atatatttga ctcattattt tgcctccttc aataataact gatttttaat
    54061 agatactgct cgtaattcat tgaccagttc caatggattg ttggaaaaga ttacttgatc
    54121 caaccatttt ttatccataa aatcctcttt tgcaaaagtt tctaataatt gttgcaagcc
    54181 gctgtaataa ccgccaatat tcaatattcc gattggcttg tcgggcgtca gaccgattgc
    54241 tatccaagaa agaagttgtg agaattcctc caatgtacct agacctccag ggaaaacgag
    54301 gacagcatct cccttttcaa ataataatac ctttctttca tcgatcgttt ctgtaggaat
    54361 aaatgtagta acatcttttc caggcaattc agtttcaaaa agcccattcg gatagatacc
    54421 tgtgactttt ccgttatttt ccagtactgt tttactaatc actcccataa tcccggagta
    54481 tgaaccaccg taaaccagtt cgatttcttc ttgtgccatg tattttccta acgtttgcgc
    54541 aataaatttg taactttctt tattaccaaa acgggaaccg caaaaaacag taagtttcat
    54601 aaaaaaacac tccctaactc aaaagtctat cttcattcta cacgtgtttt tttaaaataa
    54661 aagactagat tttgtagttt tttagtggta tataggatag acgccactat atatagtatt
    54721 tagaaaaaaa tcatttttca gccattttat agataaaaca aaaataaata acgtgttttc
    54781 atcgatatga aatgacttac ttatagaaga tactgggatt tcgtcctata tgccaataac
    54841 gtgtattatt ttaaacaaca aatacttgtt tcttttatta acgaaaaaat cattttgttc
    54901 tcgtgttatg tttcactgat aaatgatgtt tctctaaata aaaaagccga cgaactaaac
    54961 gatcaaaatt tgatcatttt tgttcatcag cttctcctaa aaatcttttt gttaacgata
    55021 aatagctgga tttgtaaaaa tatcacttga atcgtcactg ctggaagaaa attcagtgat
    55081 aaccgcacct gttgcgcctg cctggaacta gtgtttgata ttgggctcga acgtgtactg
    55141 atctctagca tgaagaataa tttctttttt tactgtataa taactttctt tcctttttgg
    55201 aatagtacag gaaagttttt ttgtttcctt tccttcgaca taaggtatac cagacccttt
    55261 cgacaacgaa aggttctcat ttttccttca tcgtcatttc aaggagggtg ccgatgctct
    55321 ggacagtttc gatcgctcct aaaccaaaat ctgtatattc gattctttct aactctgatt
    55381 ctttcatacc attcctgtct caaggtacat ctattgtact tttttcttgt aatccattct
    55441 gcctctccta actaattgtc aaatcatccc aagatatgtc ttcttcctct ttttcttctt
    55501 ggatgatttc tgcattcgga tctactggtt ttttcaataa taaaatggtt attcctgtga
    55561 caaaagaacc agctaacaat gcaccaatga atgcccatgg tctggtcatc gtagcgactg
    55621 caaacattcc cgatgccgga atacggctat ctgcgcccca aaacatgctt aatccgccac
    55681 ctactgtccc accgactccc gttgctatga cattgcgtgc caagtcgttc aatacgatag
    55741 ggaaacatcc ctctgtgatc tgacagatcc ccataggaaa agcggtcttc aatgtatcga
    55801 tttcttgacg attgagtata tttttacgaa tgattttccc taagaaataa gcgatcgtca
    55861 agccgaatgg ggttaccatc gaagctaaaa tcaatacagt gattggttca tagattcctt
    55921 ctgaaaatcg tgccacaccc ccttatccag aataaatggt aatccagaaa cagccgtgta
    55981 tctgcggtaa taagcctaac caaacgaaaa atatgagaag ctatttttgt ttggctgttc
    56041 ttattacttg atgcagggca gctttttcac aacctctttt tttatttatg tttcataatc
    56101 aatatggtaa ggatgcagct ccgtatggta gaccgcctcc gaatattcaa tcatttcccc
    56161 aatttgcgta gacgatttcc gtatccgttt gatgccgatc tgtgcgtctg ttttcagaat
    56221 tccttgttct tcttctgtta aaaatactgc cgagaaatca tcagaaaatg agtcaatagt
    56281 atatcctttt ttcgtcagca aacgatacaa agattctttg ccaaattctt ctctgctgaa
    56341 ttgcatcatt tcttgtggaa gataatgaac aaaataaata taaggctgat tgttcaaata
    56401 atataagcgc ttaaaacaaa cagcttgctc accgaaaaag gaaaacaaag gatcttcctt
    56461 ctttatatgg accaattgca aatccagcgt ttctttccgg acttcatatc gggagttctc
    56521 caagatttgc gtaaagcttg ccgctttgga taatcgattg taaggacgat tgcttagaac
    56581 cgtcgtccca cgaccacttt tctttgcgac atattcatct gatgccagca gttcgattgc
    56641 ctttcggaca gtgattttgc tgacttgaaa cagttcttct aattcacttt ctgtcggcaa
    56701 catcgaaccg actggatatt ttcctgagaa aatatcttct ttaattttat ctgcgacttc
    56761 caagtatagt acacgtgtcc ttttcataac gtcacccttt gctttactat tactaaaaga
    56821 atagcacata aaaaataaaa aaagaacaca aatcacatgt gagaagtgtg ttctttttca
    56881 ttcagctgct ttaattacaa gtttacgcca tttgaggcaa ttacttcttt ataccaatca
    56941 aaagattttt tctttgatcg attcagtgtc ccatttcctt catcgtctag atccacatag
    57001 ataaagccgt aacgtttact catttctcct gttgatgcac tgacaagatc gatacatccc
    57061 caaggcgtat agcccatcaa atctacaccg tctgctactg cattagccat cgcttcgata
    57121 tgacgacgta aataatcgat ccgataatca tcaataatcg tgccatcctc ttccatatga
    57181 tcaatggctc caagaccgtt ttcaacaatg aataatggtt tctggtaacg attgtataat
    57241 tcattcaatg cgattctcag cccttctgga tcgatctgcc agccccattc acttgcctca
    57301 agaaacggat tttttactcc tcccagcaag ttcccttctg aagttgctgc ttgtggatca
    57361 gtctcattga ttgccgtaga catataataa ctaaatccga tgtagtccac tggatattct
    57421 tttagcaact ggaagtcttt ttccgtttgc tctaattttt ccggatcgac tccatatttt
    57481 ttatgggtac gcgctgtgta tgcagggtat tctccacgta cttggacatc cgtgcagaaa
    57541 aaattgaatt cttggttttg gatcattgtt gctgcctgat tgactggatt ggcatcgatg
    57601 ctgtaagttg ttgcatagat aatcatgcag ccgatttgga tatccgaacg caattcgtgg
    57661 ccgattttta cggctttgct acttgcaacg aattgattat gccatgcttg gaaaatattt
    57721 tggtactctc ctgctccatt gctttttact aatccttgac ttaaagctgg aaagtgaaag
    57781 gccgaattga tttcattgaa agtcatccaa tatttgactt tactgctgta tctctctaat
    57841 actgtctgag caaatcgttc atagaagtcg atcagttttc gatttttcca gccaccgtat
    57901 tcttttgcca aatgaagagg catttcgtaa tgagaaatcg tgatcacagg ttcaatatca
    57961 tattttaagc actcatcaat cagctgatca taaaattcta atcccgcttc gttaggagta
    58021 ccttcgtctc catttggaaa aattcgtgtc caggcaatcg aaaaacgata acatttgaat
    58081 cccatttccg caaacaaggc aagatcttct ttgaaacgat cgtaatgatc gattcctcga
    58141 tgattcgggt aaatatattt ttcttgatca atcgtccagt caaattcttc actgccgata
    58201 atcgcaaagc gctgcttacc tccaggcata gcatctgcaa cggatagtcc ttttccgtct
    58261 cgatcataag ccccttctaa ttgatttggc agcagttgct ccaccccata aaaatttttc
    58321 tgaaaaacgt gattgcatcc tgatttcctc cttattttac aattgccaga acatcttcgt
    58381 ttgcaataat ctctttttgg tctaattcaa gtatatcggc aaatgcatca gtattcgtaa
    58441 tgacaactgg agtgattacg ctatagccag ctgctctaat tgcttcgatg tcaaaacgaa
    58501 tcaataaatc tcctgctttg acgagatccc cttgtttgac taagatctca aagccattcc
    58561 catttaattc tactgtatcc atcccgatat gaatcaaaat ctccgctccg tcagtcgttg
    58621 tgatccctac agcatgtcca gttggaaaaa tagtcgtgat ctctccgtct gcaggtgcaa
    58681 ctagttctcc tacttctgga tcaatagcga taccttttcc catcacgcca gaagaaaaaa
    58741 cttggtctgg tacttcactc aatggcagta ctttacccgt taaaggactt tttaaggtat
    58801 gccgtgctga agtaatcggt tttcctgcat tcgcatgttt gttttctaat tgattgtctt
    58861 gtgtttgttc gtcaaatcct aaaatcaatg tacctacgaa tgcaagaaca aaagcaattc
    58921 ctgtagctat tactgctact gtcacatttg actctacacc atccaccgta ttaatgaaag
    58981 tcggaatcgt caataagctg accaaactgc tggagaaagc ttttactccg gaaaatccga
    59041 taatagctcc accaatacct ccagaaatac aagcggcaat gaatggtttc ttcaaaggta
    59101 atgtcacacc atacacagtt ggttcagtga ttccaaataa cgaagtcact gtagaagaaa
    59161 tccctaatgc tcgaagtttc gtatcttttg ttcgcaatgc tacagctaaa gcagccccac
    59221 cttgtgccaa gatggctgga agaagcattg gcatcaatac atcaaaccca tattgttcga
    59281 tattcaaaaa catgatcggt acgaatcccc aatgcatgcc gaacatcacg aatacttgcc
    59341 ataaacttcc catgattaga cctgctacga ttggactaaa tccataaatc gagttgaaga
    59401 gaccgcctaa agcattaccg gcaaccgtcc cgattggtcc taaagcaaga aatgttaatg
    59461 gaaccatcac tactaaaacg acaagcggaa ctaaaatcat ttgcagaaat tgcggaatca
    59521 ctttttttac gaatggctct aatttacttt gaacccatac ggctagaatg attggtatga
    59581 cagaagaagt ataaccagat ggtgaaagaa tcactgggat cccaaagaaa ctgatagctt
    59641 ctcccgcacc agcaactgct gtgatagctg gatacacaag tgccatagca attgctactg
    59701 ctaaaaactg gttggctttg aactttttag ctgctgtaaa agcaagcatg acaggtaaga
    59761 aggtaaagac accatctgct gcagcaaaca atatttgata agtaccgcta tcagctgtca
    59821 gccagcccat caccgttgcc aatgataaga atcctttcaa taccccggcg gctgccatcg
    59881 cccctagaaa aggtgtaaat atactagaga taatatcgat cagacgattc aaaatatttt
    59941 tggactcttc ttttccatcc tccgaaactg caagaccttc ctcatccacg atcgattgat
    60001 aaacatctgc tacattactg ccaatcacta cttgatactg accgccactt tgtacgactt
    60061 ggatcacgtc cgggtcttct tttaactttt gcgtatctgc taaagattca tcttttaatc
    60121 tgaatcgcaa ccgcgtagca caatgtacta aacttttgac attcttttgt cctcctaccg
    60181 cttcccatac acgacgacca actgcttgat tatccattgt tttttccttc tttctttttt
    60241 atttaaaaaa taaaaaccca gattgaaaaa aacagtcaca aacattcgtg aacctatttt
    60301 ttcaatccag gttttgcctg ccatacagta acaatcctta tgattggatg ctttttttct
    60361 cacttactac tctttgaata tgaatcgtta aataaatctg ctctgcgatc cccatttcct
    60421 gtttttgcgt ttgtcgaaga tactcattga tccgttcgac acaatgaaac gcttttggat
    60481 actttccttg aactaatgcg tagagaaatt catcttgctc gtcagatcct tcccctttga
    60541 gataacgctg ggcaaaaaac tgcagatgag taatgatacg ctgataattc aaagattctt
    60601 catcgaacac gatgccgaag aatttactga tgatactcag tatatctctt accattgtgg
    60661 tagcggtcat cgtcacttgc atgtcttcgt ttccttgttg gctattcaca aaatgaaagg
    60721 caataaaacc ggcttcatcc tcagaaagtt ccacacccgt cttttgtttc acgaacaaaa
    60781 gagcctcttt cgctgcttga tattctttcg gataaaattt ttttgtttca aataataatg
    60841 gattaggcag ttggatattt tcttttgctc gtgtgatcgc ataatgaaga tgatcggtca
    60901 atgtcagata aatattaggc gaaaattcaa cttttagttt atcttcagcc agttcgacta
    60961 cttgtttgac ccattcgatc tcctctgccg gaatatcagc catcagctgc tggaattggt
    61021 ttttagcaaa agaatctctt aaaacaaatt ctttttggac cgcttctggc gaaatctcgt
    61081 ccccaatctt tttttgaaaa gctagtcctt ttcccataca aacgacttct tccccgaatc
    61141 catttttcga aatgacgaca ttgttattca aaattttatc aattttcatt ttgcttcgcc
    61201 ctctcaagaa aaataaaaaa acctagaatt cgcctactgt ttcttagctg aattctggtt
    61261 ttgcctgccg tacagtaaca atccatttct tattcaaaat catcatacca tcgaattttt
    61321 atttatgcaa gccttttcat ataaaaaata aaaaaactgg aacacaagtc tatctagaaa
    61381 acaaccaaat cagagatgca cctctttgcg tttctctttt tttcagcaaa ttggtttttc
    61441 cttatcgatt catatccttt ggctgttttt aatctgtaac gacttatgtc ccagtatcct
    61501 aaaatgtttt tgtctctaga ttgaaagcag atctcatatc atctgtcgca tcttttatcg
    61561 atgtaacact ttccagttct ccttgtacaa ctagtcgcgt gacggcgtcc atgtcgccac
    61621 aagtaattag tgtgacaatt tcttttccct cgacttcatt cagtaattgg gtatctgtag
    61681 gagcaacttt ttctttaaaa aatgttttgt atgtatacac attttctaaa tctgtcaaat
    61741 agatcttggc acccatctct aaattttcta gcggtgtaaa cagcaattca gggttcgttg
    61801 cacgatggct tgctaaggca taatttcctt ttcccatttc ttgatctgga gataatgttc
    61861 ctgctccgta aagtagtgac gtattatcca agcctttgaa aaccggtaaa ttgattgaaa
    61921 ctgaagggac tgcgatacta gcaatcactg gtagatctgt tcctctcatt tgcgacctca
    61981 tgaccccttc tgtcgtcatt ggttctaccg catcaaagtc aaaactgaca tctttatcgt
    62041 tattcttttt cagatcttct tttgttacat tcgctatagc atatttatct cctgtctctc
    62101 tgacaaaata atccttgatc tgttcattga aaatcaaagc tagtcctact aataacaaga
    62161 ggaacaagag gatattgact agccatcttt ttgcttttcc tttttttttc attttctctc
    62221 cctccagact cattttactg tttttctttt gctctttcca acagccgaat gacacatttc
    62281 tacaactctg tgtatttttt tcgtacacct gcgctttttt ctacaggata tttcaaatta
    62341 tttttgatca atttttcttc aatgatctca tctagatcca ggtgaaggtt gtctgccatc
    62401 atgtatgaat agatcaatac atctgccagt tcttctttga ttcgttctaa ttgtgtttct
    62461 tttacttctt ccggcgtttt ccattgaaaa agttctaaca attcagatgc ttccaaagaa
    62521 atagagatag ccaaatcttt ttcgttatga aattgtcgcc aattacgttc atctctaaac
    62581 tggttgactt tttccatact attcatttgc ttgccttctt ccaatcactt cttacttgat
    62641 taatcatacc atttttttac ataaaaaata gatgatgctt ttaaaaaaac tcctgttttc
    62701 ttttatttaa aataaatgtc atagaaaatc aataatttag gtagccacca tgtgtatgca
    62761 gtagcgtgtc ttcttccctt gaggcttttt gtttttcgat acgttcttct tcccaaacag
    62821 acaacgctac tagaatctct cttaatgatt ttccttcttc ggtgagcgag tactctactt
    62881 taggcgggac ttggttatag gcttttcgat ggatactttc atcttgttct aactctctta
    62941 actgctgggt cagcattttt tttgtgatcg tagggatttg cttgcttctc cacgcctaaa
    63001 ataagcgata ttgctcttcc ctttatctgg tgtaagcaaa gcagttagca ttttgacgat
    63061 cgtgggttcc gcttttcact tcttatctac ttagtatcat gaagtatact ttcgtactaa
    63121 tactaagtag cggaagtgtc aactactttg tttaactaca tagcaaaaga aacagaaggg
    63181 tgacaacggt catcacgctt ctgtttcttt tgaatatgct gtgttgttgt ctctatctcc
    63241 gtgtagactt tcctcaaact tatcctttga cagtaagtca gtaggctgat ttgctctttc
    63301 tagatgccag atacatttct acaaaaaaat agtgattctg ttttgaaaat tcgattttta
    63361 gttctgcatt ttcgtaaaca tccaacattt ttttcgcact gtatagacct aacccgccat
    63421 tttttttaga ggtttttcct cttttgacca tctccgatag cgtcaaattc gttggatttg
    63481 ccttgttcgc tatactaaaa cgtgatccct ctttttcttc cagcagattg atcgtaatcg
    63541 gactctgatc ggctacacga tgatcgaatg cgttagaaat aaaaatagat aaacagcgaa
    63601 taaaaacaac cagatcaatc gggattgttt tcacttcgac aggaataact acttggatcg
    63661 ggattttctc cgattctgct ttttctagaa attttaaaaa gacacccctg acagctgggg
    63721 atacaatatt tgttagctgt tgtatttgcg gtttttcaaa aatagctgca gaatgatctt
    63781 ctaatatttc taaaaattcc aacgtttctt ttttattttc ctgaagagta atttttaatg
    63841 aaaacagtag acttttataa tcatgtttga attcctctag atgatggtaa tattcttcat
    63901 ttatttgata cagttgaagc gattcctgat gttgtctttc ttttacttgg aaactgatca
    63961 acataatgga aaaaaacaaa gaaaaaaaac tgatgactat cgacaaaaga gatagaagca
    64021 gataattaga tgagcggaag tgaacacttc ccaataccaa gtaaatcacg ccccagctta
    64081 ataaaataca aatacctcca aaatagtgtt cagttggata agtaaaaagc aagttgagaa
    64141 atctcgtctg tttgtctata ctttttacta atagatagaa aataaatgcg ataagtagtg
    64201 caaaaaaact tgctgttcca agatgtctct ttcctaaata atccatcaga tcaaaaacca
    64261 gaaaaaagct gagtccagtg atagcaaaaa aaagattact gatcaaacag ctgataaaca
    64321 ggtttttcgt agtaaaataa aaaagaaaca tcaataataa gccaactagg taaggaaagc
    64381 tcactttagg gataggaagt aaaagtgtca ccaccatcaa agctatacaa ccaaagctgt
    64441 tttttactag cagttgtttc gaaaagaata aatagatacc taaatctaaa atcaaagtaa
    64501 aggtcaaata aagtgtcact gttgcgacca tcttattcct ccattgtttt gtttcaaaat
    64561 gtacacatat ttttttaata ggagcctgca tatgaaaaag tattttatca tagaagataa
    64621 ccagtttcac agcgaatgga tcatcagaga attggtcaat catcatatcg agaatatcgt
    64681 gcagctcact tctatctctc aaatcaatca gttgatccag ttgggcacta ttgataatca
    64741 cgatgttttc tttatcgata tcgacttgaa gcaatcactc acaggtattg atgtcgccga
    64801 acaaatacgt aaaaaaaatg cacatagtaa tattgttttt ttcaccgctt atacgaattt
    64861 tgcagtagag atcatcaatc ggaaaatcct tccgattgga tatatcagaa aagacaggaa
    64921 tttatctgaa caaattcgtc aagtgctaga acagatcaat tggaaagaac agcagatcgt
    64981 aaatgtcgat aaaatcttta ttgatcaaaa aggtggaagt ctcgtcctgt atcctcggga
    65041 aattttatat attgaaagta tcaaaggttt gaaaaaccaa gttatggtca aaacaactac
    65101 agaagataaa ttagtgaata cttctttaaa acaattgaag acagagttag aaaagtatgc
    65161 ttacttttta cttttaaaat cttactgtat caacttatcg caattgcaga aaattgattc
    65221 tgctcattat gtgttagaat ttttcaacgg tgactcctta ctcgttggtc gaaagatctt
    65281 tgaaaagaca aaggaacgtt tccatgactt ccaaaaaact gcttcgagtt gatgaataag
    65341 agctgagacg taagcatagc agatttcagg gagtgggcct tgaatcggct ccactccctg
    65401 aaatcgttga tatgatttta cgcttcctct tctgtaggag catctgttaa cgaccatgtg
    65461 aatgttgaaa cgtagttgtc tacagcataa ttggatacag gaacaaataa ttccactgat
    65521 tgtctccctt gttccacatt ctctcctaat gaataaatcc atgtgccaat cccttcattt
    65581 gctcctgcag ttactagatc tactgcctga tctgctggga tttcgattcc ttcagcgcca
    65641 gttccagaaa cggtagatgg cattccactg ctattagttg cgctatttcg aatcacggcg
    65701 tttttgaatt tcacctttga accagttagt ttattcgtat cagatgttgt accaataaat
    65761 tctgttcgtt tgactgataa tttccaaccg gcataatttc cgcttttatc cgttacttgt
    65821 acgaaattct gtacttgtgt ttgtgctcca gtactgtccg ttccgctttg taatgcagca
    65881 tatactgttt ttgctgtacc taacttgatt gttccaaaat caagatctgg agcttggtca
    65941 atcgtcagta atccggatgt tccctgattt ggttcgtcag gtgtaaaagg agtatcagga
    66001 tcatttggat caacaggagg aactggaaca acgtcttcag gatttgcagc atctggtttg
    66061 gtcacagaaa tcgttccttc tgtagtggct gagctcttat tctcagcaaa aacactttgt
    66121 gtacataata atagacttcc agctagaacg gtacttaatg caattgtttt tttcatttct
    66181 ttttccttct ttcttttaaa ataaataatc cagcaaataa gccaataaga atgactccaa
    66241 caagtatcag ccatcgccag tcaaatgttt ctggctgatt ttttaaatca atatcctttt
    66301 cattcaattg gtccgctgca tcatcagtta tttcaaaatc ggtatggaga ttccagcgtt
    66361 tttctttcca agcacccttc acaaccgctt gataatgtcc cggagctaat ttttttcctt
    66421 ctaatgggac aagtaaatca aaatttgtat tgggtgctac atctattttt tcttttttta
    66481 ttgaaaggta aggctctttt gtttcttttt tgtagatttc aaccgatgta gtcaagccga
    66541 acatataaga agcatttgga ttttgaagat tcgccttaat cacatttctt ccatcgcttt
    66601 gatccgcata agcttttttt aatttcaaat ctggggaaga ctctttttcg ctatttcaaa
    66661 tcaagattgc tttcacttga gaaaactgat tgacgacacc aactgtactt ttgttagaaa
    66721 cgttctcttt tttttcttga aacgtgatcc ctcctgcgac cacaccagcc agctcttttt
    66781 ctggcgcttt cagtgaaagt gtctcagtga ccgcactttg aggaggaaca gttatttctc
    66841 ccactgtttt tgctaagttt ttcatatcat aacttaatgt ttcatcaaat ttactttttg
    66901 attctccata ttcgatcaca ccgtttgaat tcgtcgtagc ggagtggatc aatgtctcaa
    66961 ctgttactga tttatctgtt cggttaaaga ccgtgtaggg gatttcctgt gttttcccag
    67021 gctccatcct tacatttata tatgttttgt tcttatcaat ctgattagcc ggtaattcaa
    67081 aatctaccgc aaaattgaat tcggaggcat ggacaggtgt ctgccaaagg atgaatccaa
    67141 ggagaaggat cataagtaaa gagcagttgt ttcgatgact tttattccat attcgattca
    67201 tcaatcatcc tccttgatct ttattctgtc ggcgcatctg ctaatgtcca tgttatctct
    67261 gctgagtaat taccagctat ctgattttct ttggttaaga caagtttaaa cgcccgaccg
    67321 ttatttaaat attctgaata atctgcttgc acattcgttg aattttgtcc tctgacaatt
    67381 tccagttctt cttcattcac taattgatac tcctctcctt tcttgaaata taggacatcc
    67441 tgcatagaaa atcctgcatc atttgtgaaa ggctgagata attttatcgt taagatccac
    67501 ggatttttcg tttctcgctg atcataaatc cataattttt cctgtatatc catttggcca
    67561 aatgcttctt gtgtcaaacc agttgctttg atctgaccaa aatcgagttt ggaaggggca
    67621 agaaatgaga gaccaccttc tactacgatc ttcgtagcag gtgcaaaagg cggggcatca
    67681 tgataaacaa ctggcgtatc acttggattg tgattcccaa tttcatcgtt ggtacttggc
    67741 ttgttctgca cacccttagc agtagcttct cctgcttggt cgtttaatac aatttctaat
    67801 acatctcctg caaaaagttg attttctggg atagaaagaa caaattcccc tgtatcatca
    67861 atagttgttt ctgacaaaac agcttgattt tttttcaatg aaaccaaggc tcctgcttca
    67921 cctgtacctg tgatcgtgcg agaagctgca ttgatcaatg tggtacttaa attagcaggt
    67981 tcaggaggaa tcacatcgac tgtcgttttg gcagagtcca gttctgtcac ttcttggcca
    68041 cctcttactt ttttagcagc aacaacttgc acttgatcct ccgtttttaa tctctcatca
    68101 ttgggtactt gaacttcgaa caagccgcct tgttcgcctt ctccccatgg gttatatcct
    68161 gccccttcat tttcatctgt ttgtccttgc gtgagagcag taatttctac agatgatttc
    68221 gtcgtatctg cgtaagtaat acgtaaagtc acttctactt cgcctgtcca agcatctctt
    68281 ggctttgcct cgacaccttc gggaacaacg acatgcccat agattttttt atctgcattc
    68341 gttggcactc ttaaggattc aatcatcggg agctgattat tggcactgat tcgattataa
    68401 tttttcattc cggcacctgt ctgagcaaat tcatttgtta aacctgcact agggcttgat
    68461 acgaaattgc ctaagtcggt cccattgatc gataagtctg tcatatcaga aaaataatcc
    68521 gggtcttttt caaagttatc gaatgttgcg cctgtttttc gccataaagc gatttctgaa
    68581 tgggcgatct ttaaagaaga atctccattt ccttggaata cccagccgcc tatgccattg
    68641 gtctcgcttc caatccgttt gttttggaaa tcaaaatagg taggagattc taagtccaca
    68701 ttcaacacat ccccactgat gatgccggtc ttatcttccc ctgttttccc gaccatgact
    68761 aaataagttt tttcacctgc cgtaaaattt aaatcaatat ctccagttgt atctacagtt
    68821 gctccggaat ctgcgcgtat atcgatattt gatttttctc cagtgactgt aaaatcagct
    68881 gaacctggac tttctagaaa accagaagcg tcataaaatt ggatcgcttg atttccttga
    68941 ttgagtctgt tcacgtaaga tttgccatca cctgtatttc ttaccctcaa tgagcctcca
    69001 ttggttactg tcagcttttg cgtaccattc tcaaaacgaa ctgcgctgga acgcccactc
    69061 tgcttatcga tgtcgataac tgatccggct ccttctacga tcaaagaaag gccttttcct
    69121 acaaaacgca tcccattgtt cccgggttga tcgtcatctc cagccatttt gaccgagatt
    69181 tgtccaccac ttgatacatg gatttgacta gaatctcctt gcaagcgaaa tccgcccgta
    69241 gttgctgtat tgatggtgat tttcccacca ttcgatacag tgactttcga tcctgaacct
    69301 ctcatttgga tgattccgcg attgttgtca ttggtaatat tgatctttgt atttaccgaa
    69361 aattcgcttc ccgaaccact gacttcaaac atgacattac caggtagcgt atcgactgcc
    69421 tctccttcat cgctaacgcc atctgcccga tcataatcaa gagagacctt tgaaccgctt
    69481 aagatatgga ttcctggcgc tgtttttcca ctaccagtct catctcctga atctagaaaa
    69541 ccgtagaatt tccctagata cggtccataa agttgactac cattgatcac attcaattga
    69601 tagcttctac aaaaataggc tttacccgga gcggcattca gtccttcctt cactggttgg
    69661 acatccaaca caccggaaac accatctaat gtgacagttg cacgaggagc gtaaatcagt
    69721 cctaatttgg acccatcata caaatcaagt gtcccggtaa attttatttc tcccgtaaaa
    69781 tgatatctgc ttccccaaaa atcggtcata taagcaatga taatggcatt tccatcgata
    69841 tttggtgtca atccacctgg atgtgcagct gttcctttaa aattctggaa ctcgatcgac
    69901 tgaggtatct cattgatata aatatggtgt gtgccgaaat tgtgagcgaa tccaccaaaa
    69961 tcaataacga tatctcgttt gatctctaat tccctgtcta aagtcaaagg ctgaatcagt
    70021 cggatcttag ttactgattc gtctgcgaga agctgcgcca gttcttctgt tgtggcagct
    70081 tcgctgtacc catccgctga aacagctctt gccagtgccg cttcactgct atcagacgtt
    70141 tgttcagcta cttctgaggt ctcacttgtc ttattttctt cttgcctttt attagtagaa
    70201 gcctcacttt gagattctgt agtttcagaa gtaatcgatt cttctgaaaa agtagtcttg
    70261 tcaaaacttt gattctctga ttgcgttgat tcgtttgttt tctctacatc ggcaaaaacg
    70321 gatgtcacca tcaagagagg gaagacaaac aaaatcgacg caatcagctt ttttacacat
    70381 tgattcattc tccaccatcc tctctgacgt tttgacaatt ttattttatc gttttaatct
    70441 atttccaatt gtactttcat atttaaaata tgtattttta tggaaaaatt tttctattag
    70501 gtgtaaattt cttttttgta caaaaaaaag gctgtgacaa aagtcttgtc acagtccctt
    70561 atcccgaaca agcggtgttc tcccgccaac tcctcggtca caacctccat tttaacaata
    70621 aacctgtaaa tgtgagggca atactttgat tgtcacaggt aatttatccc cttcatctcc
    70681 atcaacattc gtcgtcaatt cttcctcatc agctaatgac acaactaatt gctcacaagt
    70741 aaggtaggct agatttttcg tagattcatc tactcctttt aataggtctg gcaatgtttt
    70801 aagcgtatcg atcaaagaag aatcttttaa ataaaccaaa tggagcttac catcatccac
    70861 ttttgcattt ggaagtaatg tttcaaaacc gccgactgag ttagttagtc ctatcagcaa
    70921 ggtactgctc tcaatttctt ccttctttcc atcaacttct acgtgaaacg agtaggattg
    70981 tgtggaagct aactgtttga ttcctgacat gaaataagct agttttccaa atttcgtctt
    71041 cttctccgca tctacatcat tgattgattc agggatagag ccaattgcga cgacattcat
    71101 aaaatagtcg tcatttattt ttccaatatc caatggcttg actgattcaa tcgaaaaatg
    71161 attgatcgct tcctcaggtt ctagcggtat ccctaatgct cgagccaagt cattcaccgt
    71221 acccaatggg aaaaatccga aatttggccg atgttcttgt tcagcgattc cactgattcc
    71281 ttcgttgact gttccatctc cgcccatgac aaacacacta tgataccctt ccgtagccgc
    71341 ttcgcgagta aagttttttg catcccctgc tttttttgta tgcaagacga ccacttcatc
    71401 aaacaccgat tccaattttg ctatcgctaa ttgctcaaat tcctttgctt gttcaccgcc
    71461 tgaactgggg tttaccacta ataaaacttt tttcatcact ttctttccct tctgattgat
    71521 ttctttaata taacgatagc ataaagaaaa aaattaggct gtgatgaagt actgttcatg
    71581 aaacattcat ttattttttg acaagataaa acatccatac tatgaaactg aggaaaatta
    71641 aaacaatgat ataggaaata aaagaaaatg ataggcattg tatacctaat aaagtaaaaa
    71701 tcttaggtgg cagataaaaa cacaaaaata gcaagagaat gtaatagcgt ttcttccact
    71761 ttttgttatc agaccttata gcgatcagag gaataccaaa tgcataactg aaaaactctt
    71821 gatgtactgt taatatgtca cggccttgaa taataaatgc gacaatcccc gctataatta
    71881 gtgtgcctaa caccaaaacg gaaaattgat gaatatattt tatatacaaa tgatccattg
    71941 tacttcctgc ctttttattt gtgagaaaaa caaatccgta tacgtgaata acttctagaa
    72001 ataaaagaaa tttgcaaaac cactccattt atctacttct acgtgcattt tttgatattg
    72061 ttttctttat ttaagattat acagaaaaat atctagggtc gctggattct aagtaaaaaa
    72121 aatttttaaa ataaaaagaa ctaaatcaag tagtaattaa actactgttg atttagttct
    72181 aataaaaaga tttagatgaa cagttttatt gatttaaata gttcatattt tttatttata
    72241 ccgcttattt actacttgct gtactagaat ctgaagtgaa tgtactcaaa acattttcaa
    72301 agtctttgtc tttgattttt acattagcgt ctttcaatac ttcgccaatt actttattct
    72361 ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc tttcaattct tttttgtatt
    72421 tatccatgtc gttgcctttg ttttggtttt tcaccatttt aaccacataa tattccgtag
    72481 tataagttga agcattggta gaagtgatca catcagaaac ttgaccatct ttcaacttaa
    72541 aggctgcttc tttgacttct gccggaaccg ttgtagtagt ggaatcaaat ttcacttttc
    72601 cgccatcttc ttttgtcgta gtatcagttg acttgtcttt ggctaattta gagaaatcgt
    72661 ctcccttatc agcagctttc ttcacatctt ttgcatcatc ttcacttgct acttggataa
    72721 tttgagcttc cacttctgga tggaaagatt cccaagccgt ttttaaatct tcatcggtca
    72781 atttgatatg tttctttaga ccttcttgaa aggctagact ttgtttgata ctgtctttga
    72841 atgtttgttc tgtgtaacct gcagatttca attgtgaatc aaatgaatca cccaattgtt
    72901 tctttgtttg atcatattga gaatcgattt ctttggttga tacgtcatct ccatatttat
    72961 cttcaaatac ttgataaacg atcaactgtt gcacagcgga atctgctcct tgcatttttt
    73021 tcaagctatc cattacttct tgttcgctga ttgtaccgcc tttataacta acggctgttt
    73081 tttcaccggc attcgtacag cctgacaata agccagtgcc tagtacgatg gcaagtgcta
    73141 aaaatctttt tttcatttat caattctcct acttttcgtt ttttgcgaat tgctaaaaac
    73201 aaaggtgttc aagatgaaaa tcatcaagaa cacctttact aatctgctcg atacgtcctg
    73261 aatagatata aggctacaca atccagaaag ttcgctcagc agaataaatg tcaagtagca
    73321 catcacacct taatatacta cttcttttgt taccgttttt caagtctatc ttgcgttccg
    73381 tatttaacgt aactaattct tagaacaaat gttatcgatt ttgtttttct tcttccgctg
    73441 ctgggatatt caaatcagac acttttatgt tcgattttcc agacattttt ttgttgtttt
    73501 tattatgata tatttttata caactaaaaa tgaatatatt aaaactacca aaaataactt
    73561 ttaacgctgt ttcattatat gttttttcta ttttatataa acttttggat acttaatacc
    73621 aaccggttgg attgtaccaa catgagaatg tgtcagttct atttcctgtc gcacttgcat
    73681 aggcccatac tccttttgtt gcatttgctg atcgaataga gtttccaact ttcgcttttg
    73741 caccgtgacg attatagaca cgactatagt ggttagaatg acctgttttt ctcgtacaag
    73801 ttgtatcact ataataaacc catcttccag agctatgatt aattgctgtt ctagaaattt
    73861 cccggtctac tggtaaattt gacttaataa tattaccttt agcatcaaaa tgaatctctc
    73921 ctggctttaa agatttgtaa tagatttcac cgtgaactga atttgcttcc acttcagatg
    73981 acatagcgtt tcctccaaca aaacctatcc ctagtgcaca aataataact gcttttttca
    74041 taaaacgaca tcctttcctt tttgattttt aaagcatgat cttcttgttt cctattttgg
    74101 tcagatatgg atcatgcgtg actacgataa ctaacgtacc ttgggaagct tgttgcgata
    74161 agatttcaac tactttatcc ttatttcttt tatccaatga cccagtcggt tcatcggcta
    74221 aaatgatttt gctattcttc aataagactc tcgcacaggc gattctttgc ttctcaccac
    74281 cagataatgt gtagataggc gtacttagct tcttgtttat atttactttt ttcaatgcct
    74341 catactttgc tgttcgctta tctgaaacat ttaaattcga aaacttcaat ccaatatcta
    74401 aattaaaatc gattgtttca tattcaatca acccaaaatc ttgaaaaaga aaagatatgg
    74461 tatttctttt tatcaagcgc ttctctctcc cagttaattg tttcgtctct tgatcgaaca
    74521 aatagaattg tcctgaactg aactgatcat tcaatcctaa tatattcaat aacgtacttt
    74581 tacctgctcc actttctcca acaacagtca caagatcccc agcatccgca gtgaaacgat
    74641 ccaaatgaag aatcgttttg tttcgaatga tttttttcgc accttttatc actatcaacg
    74701 gctccaattg ttaacctcca ttcatcagca ataactgact tttcttttct gtagacaaaa
    74761 taaaaacaac tgaaagaata aaatcaatga agctaaagaa aaaaatatta aaaagatatt
    74821 taggctctct ttctataaga aaaaggaaga ttacacttgt cgttccccat ttgatgaggt
    74881 tcatcatgaa gaaatcttta tacgtttcaa agagcgaata accacttgaa cgaaagatag
    74941 cgattttccg acaattgtag acaaaatata tgcataacat gacatagctc aacaagaaga
    75001 aagaaaagaa agtgagtaaa aatatctgta tcaagtaatg aaatgatctg cttaacactt
    75061 tgacagctat ctcttcagtc tgagtatacg gaaatgataa ctgcaagtta tcacgtaact
    75121 gactttcttc aatcagctca aataaccttt ctttcgacaa agagcctatc ttgatcttta
    75181 acggtggata gatttctccg ctgagaatat ttctttccaa atacatactg tttttcaatg
    75241 tcagtatttc aacaaccgga taatgttcga tccaggggat agaaggaata aatgtataaa
    75301 tgggttgatt gttttttata aatagaaatt tcggttcttt ttcactgatt tcttgataat
    75361 attctgttat ttgcgccagg tcatttttta aatctgtatg tctatatcgt tcagggatca
    75421 atataacagg gtgcgtttct ttttcggtta tttggatcaa ccttccagag acatcaatca
    75481 aaggaaattt cttaagataa ttcagattta tttttatccc tctgccaaac tgttcatttt
    75541 cttcattcaa gtaatctgat acattgacta atatacttcc gtttttattc aaagtttcat
    75601 ataattcctg atctgctgct gatgcaaaac gattggaata aataaaatcc acgtggtttt
    75661 tcccgatggt tactggataa aatacatgat agtcctcacc agcgatttct tttggtggtt
    75721 catgggaaac agctaactgt gaagaagaaa aaacgatagt tgctaattcg acattggcaa
    75781 cggtcaaaag aagagaaaac aacaatccaa atgtaaaaag aatgtattgg atatagttga
    75841 acgcttctct taacgaagcg attttttcaa ctaataaaag agaaatcaaa gagataccat
    75901 atattccgct gaacaagaaa acgtacatct ggaacaaata aagaaattgc tccttataat
    75961 acataaaaat aaaaccacca atgattcctg caattatgaa tgtaataatg attttctttt
    76021 tccccaataa acaccacaat acttgatgta atgaaaagtc attattcaac aataaactga
    76081 tttctttatt catcaataag aaaaaggatg ataacaaaag gaataaaaga atcaatccaa
    76141 tagttaaata cactcgataa ttatatacgt ctgtatttga aataaccgaa tacgaatagc
    76201 tattttcaaa gtcttcaaaa atattagtgg cataatcagt gtgaaatgct tgattgaatc
    76261 gatcagtgaa tagtgaaatg aaccgttcat aggcttcttt ttctctcgtc atcaagaaaa
    76321 attcaccttc aaaatctttt attttgatcg attcttcaat ggacgttacg cgaaaggagg
    76381 tattagaata gggtaccttc tcgtgagaag atttgacatc cccttgataa tacaatgtca
    76441 tttcaagctt tggctgtatc ttgaagttcc aagatgtatc tacttcattg gattcattta
    76501 ctgttttttt catataagaa agctcagttt ctgatgagac attttcaaat accatgatta
    76561 gctgctcata ttcttcttgc ccaataaaat gttgtggcaa tcgaatcgga gtataagtat
    76621 ctgtttgatg gaaaaattca tttgtccgat agagtacctg tttatttata ctaaccaagg
    76681 caaaaccact catgataccg gtcactatga ttgtgagtaa aactgtgaac ttttttttga
    76741 ttatcaccac tctcccttac ttaaatgatt ctttgataaa atcataccgt ttttcatcaa
    76801 aaaaacgtat ctatcaagtg tataggtcat gtagaataaa tctatatact gtagaatcga
    76861 aaggagaaga agatgaacac cggagaaaaa atacgaactt accgcagaaa ttgtaagctt
    76921 acgcaaaaag aattaggcga aaaaattggt gtttccgata aaacgatttc tagttgggaa
    76981 aattctcgta caatgccaga ccttgaaatg ctgtcacttt tacatcatac tttaggtctg
    77041 ccgattgatt ttccttctat tgaatcagct accgaatcaa tagaaccaac attgccagat
    77101 gaacctcttg ttaacaagaa atcttattca tcaaaacaac ccttctcact caaaaagaca
    77161 ctatctttct ttctgctcgt cttttgtttg gtctatcttt ttcttatcaa ttatcaatac
    77221 aaaaatgaaa cgatcgatcg attcaatcct tttttgaaac cacaaatcgg ctataccaca
    77281 ctccctgtag gatacgtgga caattccatc gaacgttggg tatgtaatga tatctttggc
    77341 catggtacat ggctgtcttt tcacgctgga gaggtagaaa aagagaaaaa ccatgctgta
    77401 atcaaacaca aaggaaaaca cgtcatgaac gttcgcctgc tcacttggga agccattccc
    77461 tcagattacc aagaaatcct cagccaagac tatgacgacg ccttaaaaag aagccctcag
    77521 gagccagata ggcattaagt aggagatgaa cgtagagata gaaaaaatca atgcacatga
    77581 tcttatccaa aaaacagggg tacaaattaa ttgaaaaagc cttgagatga aagttcctaa
    77641 ataaaaaagt tcagaatgat aggtatttct ggcttatagt ataatattta cgccacagcc
    77701 tcccaatgat tctttttgga atcataatca aggatggaca ttcacaaaaa tattttgctc
    77761 attcgatccg aatagattaa cgaccaatct agtaagtaat atagattggt acgtagtcaa
    77821 gctgcacata atttagatca attttagttc ttttttatat ttctatgatc tgctcattta
    77881 cttttatatg atggcttaca gtaacataaa taaatatgat aatcgctggt agtttatcca
    77941 agagtactca ataaaataca aaatggattg ttcaagcgcc tatcataatg ataaaatgaa
    78001 acaaataaac gatacaagga ggaagagact ttgagtaatg gcagaacaat tttttcattg
    78061 taaaaattgt tcagttgtta atctatggga tttcggtagc atgggcattt ttttatagag
    78121 gaatcacacc aacctggccg ttttatctct tgttagtggg aatattagta ggaaattttg
    78181 cccctcttca ctatcgtacg aacttttttc caactgattc tggccttttt cttaaaacaa
    78241 agacgcgaaa attagaaaac attattgaaa tgacaattac ctgtataatc attttcttag
    78301 ttatattcct aaacgtcttc taataagaat ctcttaaaat tagcttgtta cagttagtta
    78361 cctagtttat ataacacagt tatcttatct tccttatcaa catcttttgc caactctttg
    78421 tcttaaattc gaaacatata agcagagcta cgaacaccaa gactgtttcg gctctgtttt
    78481 ttgcatcaaa ttcactctta tatagctgat aatctcggct ctttaaaaga aattattttc
    78541 gcttcatttt attataaaaa ggctaggaac cttgatgtta tcaggttcct agcctttatt
    78601 taagtattac cccacagacc cttccatata gatttttaaa ttacacacta gtttaaacta
    78661 aaacaaacaa ggaaaaactt accatttaat caaaaaatta gctcatatga atatattaga
    78721 atgtaaatta gactggtcaa aatctggtca aaaaagagga cgaatacagg tagtcctctt
    78781 ttttgttatt ttgatattct aaatcatttt ttattggatt attcccttgt tcgatataga
    78841 cccaaattga tctatcaata attatcaaac tgagtcatat agagacaaaa atttcaaaaa
    78901 tgacctgtta aatggtcatc gaatatcatt taaaatcaat acgacggagt cagacacgga
    78961 gtcaaaacaa gcccgctgag agtaaaaaat atacagtccc ataggtaaag tgccacagga
    79021 ctgtatattt ttttatttga gattacctga aatgattcca tttaattttt atctcatttt
    79081 ttcctactag ttagtatcaa atactctcct attttctcaa taaactattt tgggttacat
    79141 ggtatctgaa acagctaaaa ccttgtcatt atagcaaaaa atcactttat ctccgtttca
    79201 tttcagatac atttcactta agcggattcc aaaaaaacaa aagaaaaagg aacccctagt
    79261 tagcctatgc tttttattgg ttcctctcaa aacacggtca tataacaacg gggtggggta
    79321 aatgaatgaa aaaggtggag gaaacattat tcaatagctg tcttaaatac ttcgttataa
    79381 aatgtgaaca aataaaagac atctatccct attttctaca cttccacttg actaccatct
    79441 ttgaatcgta caattttctt aactcttctc gtttacctca attcattctc aagtggaatc
    79501 aactcatcat ctaacatttt acgccatgct cgcttcattt cttgtgggta tttaaatggc
    79561 ttcgcttctg gatgtttggc tttatatcct tcgtaatcct tactgttgat aattgctttc
    79621 atattaggaa ttggatcttc acctggtaga tattgcagat tagaagcatg taattctttc
    79681 tcagaaacaa accactcatt ggaaaagaca tgaatcaaat catcactcgc ttgtgtaaaa
    79741 tagcgacgtt taatgacaaa gatatcttca ttactatcta aagtccccac atcaaatgca
    79801 tttaaaattt gtcggtaaac ttctttgact ccttctgttt tattcaattt agccaaagct
    79861 ttttcaattt cttctcgtgc atctgaacta ttcgcagcat aagttcctag taattgatca
    79921 atataagttg aatcaatatc atacaactta gtggtatttt cactataaaa ttcgatcgtt
    79981 gaaaaatctt ctccttcttt ttctggttct tcctctatta atgaaccctt cacggtctcg
    80041 tacacgccaa tttgttcttc caaaagttga atttgattgt tcaaagttgg agatttcacc
    80101 atgtcatcat tgtaatcgtc ataagtcact aacgattcat aagcacgatt caattcttga
    80161 aatgctttga cgtactcgat acggatttca agtggcgtat gttcatcaat ggattccatg
    80221 gatgaaacct cttttaactt accatatgct tgtttgaatc tttttttgga ttcctgataa
    80281 cttggataaa tcaaacctga ttcttctttt gcttttgaat acagttttgt cgcatcctcg
    80341 acattcttct ccattgtcgc tggcttacga aacgtcacaa tcaatccttt tgtcttatca
    80401 ggatacgtac gattcgtacg tgaaaaagct tggatcaatc ctgcatactc tagattacga
    80461 tccacaaata gggtttgaat cgtaggggcg tcaaaacctg ttaatagacg atccactaca
    80521 atcgctaaat cgatttgttt cccaaattgt ttgaattccg ctctttttcg tgccaaacga
    80581 ttattgatgt ctccgttata acgctcaatg tctgctaatg accaggaagt atcataatac
    80641 tgattatatt cctcaataat tttttgcatt tcttcttgtt gggcacttga atctcggcta
    80701 ttttcttcta atgaataggt aatagcgatt cgtggaaaat caggatcctc catcgttcgt
    80761 ccttcacgaa tgagctgatt gggaaactct tttgttaacc aatcaggctc tttcgtcatc
    80821 tctttgatgg cccgataata ctttttggcc atgtcaatgg aactcgtggt taagattgca
    80881 gatttcgttg gcctgccatt tcgaaaatca aattttgtat aggcattatc tggacggaac
    80941 attttatgaa tgactttttg gatatgttca tctgcttcaa aaacagctgg gtccaaataa
    81001 ctttcttttt tgacaggttc catttgatca atcatacgat tgatctgttc tgatgaatac
    81061 tctgcatatg tttctacttc ttttaatttc cgatagattt tattctctag tgaagtcggc
    81121 tccacggtgt tttcatgctc tacttgaaat cctaagacag agccatcttc tagcgcattc
    81181 ttaatggtat aggtatgcaa gacctctcca tactgatcat gggttgtacg tgccaattgc
    81241 cctttggctt gtttccgatt ctcaggaaaa atcggtgtac ctgtaaagcc aaaccaagtg
    81301 gatttcggaa acattttttt gatttcttcc atattttcag cacttaacgc tcgatgacac
    81361 tcatctacta caaacacgat atgttggccc attaattttt ggaagcgatt ggttcctttt
    81421 ttctcttctt gtttctttgc atatttcaac gccgcatcta gtttttgacg ggttgtaatg
    81481 attacaacat ttgcattggc atctgccaac agggtttcac ttaattcttt ggcacttccc
    81541 gttccaacga tcaagctatt cgctttggcg ttcccggaag aaatcccagt attaaattcc
    81601 gaagcaaatt ttgtaaactc tgtggtggtt tgattatcta aatctttgcg gtccactaac
    81661 atgatcgttc gatcgatccc cgattttcgt gctaaaagct tagtagatac aaaactagtc
    81721 aacgtctttc ctgaaccagt cgcatgccac acataccctg attgatgttt atttgcggca
    81781 ataaacaagg cttgaatcgc atgtacttga taaggatgta acaccatgag ggttttattg
    81841 tcttgatctt cactgacgat tgtataatcc gcaatcaaac gatgagcatc cgggatattt
    81901 aacacttgtt tacaaaactc atataagttt tctacttttt cattgtcttt cgttcgccaa
    81961 ctaaataaaa acttagggtg caagtctttc ggtaaggcat ttgcaaaata ccgagtggtt
    82021 tgttcattgg aaaccacaaa caactgtaag gtcgaaaaga tattgttccg aaacatacct
    82081 tcttctgcat attttttgat ttggttaaat gcctgataaa caccatcttt tgcagttact
    82141 tgtttcagct cgatttggac aatcggtaat ccattgatta ataaggtgac gtcaaaacgt
    82201 cgatcccgtg cttctatgtt acttccacgt tttgcaattt gatggaccac ttcataagta
    82261 gagattcctc caccaatgtc ttgattggaa taaaggataa gtgagacaga acccaattgc
    82321 gaatcttccc gttcaatcgt gatccgagcc atcccatttt caccttttaa ccatttcgca
    82381 gcatcaaatg gggttttcgt tcgcagcagt aattccgttt tgatcgtttc aaattcttta
    82441 tcggtcaaag gaaaatcatt cagttctgct tgattatttg agataatttt ttggcgtaaa
    82501 ttgacccaaa ggtcttcttc tgattttaga tccggacgat acgtccactg attatgccct
    82561 tctccaagta cacgaatcaa ctgttcttcg acttctgctt cctcacggtg actaattttc
    82621 attcttcttc cctcctagac aaacatcttt tgtaagaagc tttttttcgt ttcttttagt
    82681 aaatctaact tacgttgatg aagagcgatc gtatcatcta gctgtttgaa gaaagtaccg
    82741 attttttgtt gttcatcctt tacaggaacc ttaacactga tattctcaat atcagaactg
    82801 ttataagaag gcaatgcccc tacttgggag tatcttggca aatttatagt ttcaaacaat
    82861 attttaccaa aatagatatc ccaagtatta ttaaataaat aagccatggt attgttatct
    82921 attaagaaag ggcttgtgac caaacgtttt ctattaagca ttattgctgc gccaacttta
    82981 gcaaatataa tcgctggcac atcttttatt ggcttccatt ttttacgctc aatttgctca
    83041 ttagatacat aatgatttgc atttctcatt tcatactcat taccaatgtt attcatgtcg
    83101 gaaactttaa aaaatggagt accttcagaa ccaccttgct cggcatcagg aaatccaatt
    83161 cccgattgag ttttacctaa ttctcccaac ttacgctctt cccaatcttc cgtaaaacca
    83221 ggaaaccgga tttctggcac ttttgctccg ttttcaggga acattttttg taagaatcct
    83281 tttttcgttt ccttcagcaa atctaactta cgctgatgaa gagcgatcgt gtcgtcaagt
    83341 tgtttgaaga aggtgccgat tttttgttgt tcttcctcac taggaaacat tgctactata
    83401 tttttaaact tttttagaga cagaacatgt tgacctgttc cctctgcttg aatagataat
    83461 tgtcgttgga ttccattgtt aatacgtctt aagaagaagt cggaatcagc agattttaaa
    83521 gtaaatacag gataaaaata gcttataatc cctctatcaa taatgtcatt acggttaaac
    83581 acaaacacat cattatcact tcgactacgg aacgtgaaat atcctctagg tagtacaaag
    83641 taaccaatat tattttctgt agttacttgc cgatttgcaa aatagtcttc ttgcaaaact
    83701 atcccttttt gttgagatga agttagcata ggaaattgat tttgtacact tgtcttttca
    83761 acataatcta caacgaagtc ccccaacttc ctctgttccc aatcatctgt aaatcctggg
    83821 aaacggattt ctggttgtgt atcattgctc atgtttgaac acctccagcg caccttgtaa
    83881 ccacgcttca ttttcaggtg ttgtttgcag ggaagagata gcttctaaaa gttttttttc
    83941 tagcacttgc tgttcttgtc gtatcttctt gatgtcattt ccgacatgaa ccatatcaat
    84001 tggttcttct tcttcgaaag tatctacata gcgtgggata tttaagttat agtcattttc
    84061 tttgatttca tcaaaggttg ccaaatgagc gtatttctcg acatcttttc tttcagcgta
    84121 ggtttcgagg attttttgaa tgttttcttc cgatagttta ttttgatttt tccctttaac
    84181 aaattcacga ctagcatcaa tgaataatac atctcgagtt tgacgattct tcttcaacac
    84241 aatcaccgtc gttggaatcg atgtcccaaa gaacaagttc gcaggcatac cgatcacagc
    84301 ataaatactg ccatcttcta gtaatttttg acggatgacc ccttctgcag cgccacggaa
    84361 taaaactccg tgtggtaaga cgatggccat ggtacctgtt tccttcaaat gatagaaccc
    84421 atgaagaaga aacgcaaaat ctgctttgga ttttggcgct aatttcccat aacgattaaa
    84481 ccgagaatca tctaagaaag tcgtatctgc tgaccaatta gccgaataag gtgggttcat
    84541 tactactgca tcaaatgtgt aaggttcatc tgttggccag tccttattca acgtatcccc
    84601 attgcggaga ttcatttctt cggcgtcgac accatgtaaa atcaagttca tttttgctag
    84661 attgtatgtc gttgtattta gttcttgccc atgatattta acgttatctg ggtgtgttaa
    84721 gtagttacgt acgtttaaca tcaatgaacc tgaacccatc gttggatcaa aaacactaaa
    84781 gaatcgtcgt tccttttgat cgagcgttac gatttgcgcc atcatatcag aaaccatatg
    84841 aggtgtgtaa aattctccgg ctttcttccc tgcttctgaa gcaaattgac tgatcagaaa
    84901 ctcatacgca tccccgatca cgtccccatc gtgttctaaa acatctacat cattcaattt
    84961 cttgatgact tcggtaatcg tcacatttcg ttgttgctca tctgttccta gtttttttga
    85021 ttggagatcc acatcatcaa ataacccatt aaattgattg taggtagatg ccaattgcac
    85081 aaaggctttg tttagatcgt tcaattggaa cgttgcttgt tttgcttgat ccgctaagac
    85141 gttgaaaaga tactctggtg caattgcata tcccaaaata tcaacgattg tggcaatcag
    85201 atcttctttt gattcttcat ctgaaagtaa ttcacgatac aacattgttt gtttgcttac
    85261 tgtgtcatat tcctctaagg attcatctgc ta
//
LOCUS       DACDKJ010000005        82655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDKJ010000005 DACDKJ010000000
VERSION     DACDKJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.005"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..217)
                     /locus_tag="IV328_000371"
     CDS             complement(<1..217)
                     /locus_tag="IV328_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP8936541.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNK"
     gene            complement(404..1054)
                     /locus_tag="IV328_000372"
     CDS             complement(404..1054)
                     /locus_tag="IV328_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936542.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1597..2532)
                     /gene="whiA"
                     /locus_tag="IV328_000373"
     CDS             complement(1597..2532)
                     /gene="whiA"
                     /locus_tag="IV328_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8936543.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2566..3564)
                     /locus_tag="IV328_000374"
     CDS             complement(2566..3564)
                     /locus_tag="IV328_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8936544.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3561..4445)
                     /gene="rapZ"
                     /locus_tag="IV328_000375"
     CDS             complement(3561..4445)
                     /gene="rapZ"
                     /locus_tag="IV328_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8936545.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4580..5311)
                     /locus_tag="IV328_000376"
     CDS             complement(4580..5311)
                     /locus_tag="IV328_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8936546.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5315..6580)
                     /locus_tag="IV328_000377"
     CDS             complement(5315..6580)
                     /locus_tag="IV328_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8936547.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(6843..9659)
                     /gene="uvrA"
                     /locus_tag="IV328_000378"
     CDS             complement(6843..9659)
                     /gene="uvrA"
                     /locus_tag="IV328_000378"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8936548.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9669..11663)
                     /gene="uvrB"
                     /locus_tag="IV328_000379"
     CDS             complement(9669..11663)
                     /gene="uvrB"
                     /locus_tag="IV328_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8936549.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11922..13424
                     /locus_tag="IV328_000380"
     CDS             11922..13424
                     /locus_tag="IV328_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8936550.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13426..14163
                     /locus_tag="IV328_000381"
     CDS             13426..14163
                     /locus_tag="IV328_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8936551.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14208..14381)
                     /locus_tag="IV328_000382"
     CDS             complement(14208..14381)
                     /locus_tag="IV328_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8936552.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14357..15022
                     /locus_tag="IV328_000383"
     CDS             14357..15022
                     /locus_tag="IV328_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8936553.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15104..16270)
                     /gene="dnaJ"
                     /locus_tag="IV328_000384"
     CDS             complement(15104..16270)
                     /gene="dnaJ"
                     /locus_tag="IV328_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8936554.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16421..18250)
                     /gene="dnaK"
                     /locus_tag="IV328_000385"
     CDS             complement(16421..18250)
                     /gene="dnaK"
                     /locus_tag="IV328_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8936555.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18301..18864)
                     /gene="grpE"
                     /locus_tag="IV328_000386"
     CDS             complement(18301..18864)
                     /gene="grpE"
                     /locus_tag="IV328_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8936556.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(18958..20001)
                     /gene="hrcA"
                     /locus_tag="IV328_000387"
     CDS             complement(18958..20001)
                     /gene="hrcA"
                     /locus_tag="IV328_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8936557.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20235..21401)
                     /locus_tag="IV328_000388"
     CDS             complement(20235..21401)
                     /locus_tag="IV328_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8936558.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21417..21644)
                     /locus_tag="IV328_000389"
     CDS             complement(21417..21644)
                     /locus_tag="IV328_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8936559.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22128..22718
                     /locus_tag="IV328_000390"
     CDS             22128..22718
                     /locus_tag="IV328_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8936560.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            22908..23621
                     /locus_tag="IV328_000391"
     CDS             22908..23621
                     /locus_tag="IV328_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936561.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(23736..24746)
                     /locus_tag="IV328_000392"
     CDS             complement(23736..24746)
                     /locus_tag="IV328_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8936562.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(24783..25265)
                     /locus_tag="IV328_000393"
     CDS             complement(24783..25265)
                     /locus_tag="IV328_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8936563.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25415..25783
                     /locus_tag="IV328_000394"
     CDS             25415..25783
                     /locus_tag="IV328_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8936564.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26248..26700
                     /locus_tag="IV328_000395"
     CDS             26248..26700
                     /locus_tag="IV328_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8936565.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(26824..27675)
                     /locus_tag="IV328_000396"
     CDS             complement(26824..27675)
                     /locus_tag="IV328_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8936566.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(27688..28473)
                     /locus_tag="IV328_000397"
     CDS             complement(27688..28473)
                     /locus_tag="IV328_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8936567.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(28825..29766)
                     /gene="ribF"
                     /locus_tag="IV328_000398"
     CDS             complement(28825..29766)
                     /gene="ribF"
                     /locus_tag="IV328_000398"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8936568.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(29770..30693)
                     /gene="truB"
                     /locus_tag="IV328_000399"
     CDS             complement(29770..30693)
                     /gene="truB"
                     /locus_tag="IV328_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8936569.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31061..33423)
                     /locus_tag="IV328_000400"
                     /pseudo
     CDS             complement(31061..33423)
                     /locus_tag="IV328_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            33871..34044
                     /locus_tag="IV328_000401"
     CDS             33871..34044
                     /locus_tag="IV328_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8936570.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34201..34548)
                     /gene="rbfA"
                     /locus_tag="IV328_000402"
     CDS             complement(34201..34548)
                     /gene="rbfA"
                     /locus_tag="IV328_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8936571.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34575..36881)
                     /gene="infB"
                     /locus_tag="IV328_000403"
     CDS             complement(34575..36881)
                     /gene="infB"
                     /locus_tag="IV328_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8936572.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(36894..37205)
                     /locus_tag="IV328_000404"
     CDS             complement(36894..37205)
                     /locus_tag="IV328_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8936573.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37202..37495)
                     /locus_tag="IV328_000405"
     CDS             complement(37202..37495)
                     /locus_tag="IV328_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8936574.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37517..38692)
                     /gene="nusA"
                     /locus_tag="IV328_000406"
     CDS             complement(37517..38692)
                     /gene="nusA"
                     /locus_tag="IV328_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8936575.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(38715..39188)
                     /gene="rimP"
                     /locus_tag="IV328_000407"
     CDS             complement(38715..39188)
                     /gene="rimP"
                     /locus_tag="IV328_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8936576.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39333..43691)
                     /locus_tag="IV328_000408"
     CDS             complement(39333..43691)
                     /locus_tag="IV328_000408"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8936577.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(43903..45612)
                     /locus_tag="IV328_000409"
     CDS             complement(43903..45612)
                     /locus_tag="IV328_000409"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8936578.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(45680..46948)
                     /gene="rseP"
                     /locus_tag="IV328_000410"
     CDS             complement(45680..46948)
                     /gene="rseP"
                     /locus_tag="IV328_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8936579.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47109..47909)
                     /locus_tag="IV328_000411"
     CDS             complement(47109..47909)
                     /locus_tag="IV328_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8936580.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(47906..48718)
                     /locus_tag="IV328_000412"
     CDS             complement(47906..48718)
                     /locus_tag="IV328_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8936581.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(48913..49470)
                     /gene="frr"
                     /locus_tag="IV328_000413"
     CDS             complement(48913..49470)
                     /gene="frr"
                     /locus_tag="IV328_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8936582.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49473..50195)
                     /locus_tag="IV328_000414"
     CDS             complement(49473..50195)
                     /locus_tag="IV328_000414"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8936583.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50331..51212)
                     /locus_tag="IV328_000415"
     CDS             complement(50331..51212)
                     /locus_tag="IV328_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8936584.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51311..52093)
                     /gene="rpsB"
                     /locus_tag="IV328_000416"
     CDS             complement(51311..52093)
                     /gene="rpsB"
                     /locus_tag="IV328_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8936585.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52452..52931
                     /locus_tag="IV328_000417"
     CDS             52452..52931
                     /locus_tag="IV328_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8936586.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53148..54239
                     /gene="phnW"
                     /locus_tag="IV328_000418"
     CDS             53148..54239
                     /gene="phnW"
                     /locus_tag="IV328_000418"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8936587.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54232..54360
                     /locus_tag="IV328_000419"
     CDS             54232..54360
                     /locus_tag="IV328_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8936588.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54364..54909
                     /locus_tag="IV328_000420"
     CDS             54364..54909
                     /locus_tag="IV328_000420"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8936589.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55363..57054)
                     /locus_tag="IV328_000421"
     CDS             complement(55363..57054)
                     /locus_tag="IV328_000421"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8936590.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57474..58421)
                     /gene="arcC"
                     /locus_tag="IV328_000422"
     CDS             complement(57474..58421)
                     /gene="arcC"
                     /locus_tag="IV328_000422"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8936591.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58536..59555)
                     /gene="argF"
                     /locus_tag="IV328_000423"
     CDS             complement(58536..59555)
                     /gene="argF"
                     /locus_tag="IV328_000423"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8936592.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59646..60875)
                     /gene="arcA"
                     /locus_tag="IV328_000424"
     CDS             complement(59646..60875)
                     /gene="arcA"
                     /locus_tag="IV328_000424"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8936593.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            61336..62037
                     /locus_tag="IV328_000425"
     CDS             61336..62037
                     /locus_tag="IV328_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8936594.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(62646..63662)
                     /gene="tsaD"
                     /locus_tag="IV328_000426"
     CDS             complement(62646..63662)
                     /gene="tsaD"
                     /locus_tag="IV328_000426"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8936595.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(63659..64123)
                     /gene="rimI"
                     /locus_tag="IV328_000427"
     CDS             complement(63659..64123)
                     /gene="rimI"
                     /locus_tag="IV328_000427"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8936596.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(64130..64672)
                     /gene="rimI"
                     /locus_tag="IV328_000428"
     CDS             complement(64130..64672)
                     /gene="rimI"
                     /locus_tag="IV328_000428"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8936597.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(64656..65480)
                     /gene="tsaB"
                     /locus_tag="IV328_000429"
     CDS             complement(64656..65480)
                     /gene="tsaB"
                     /locus_tag="IV328_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8936598.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(65569..66549)
                     /locus_tag="IV328_000430"
     CDS             complement(65569..66549)
                     /locus_tag="IV328_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8936599.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(66573..68057)
                     /gene="galT"
                     /locus_tag="IV328_000431"
     CDS             complement(66573..68057)
                     /gene="galT"
                     /locus_tag="IV328_000431"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8936600.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(68069..69058)
                     /gene="galE"
                     /locus_tag="IV328_000432"
     CDS             complement(68069..69058)
                     /gene="galE"
                     /locus_tag="IV328_000432"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8936601.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            69306..69473
                     /locus_tag="IV328_000433"
     CDS             69306..69473
                     /locus_tag="IV328_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8936602.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(69535..71346)
                     /locus_tag="IV328_000434"
     CDS             complement(69535..71346)
                     /locus_tag="IV328_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8936603.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(71343..71708)
                     /locus_tag="IV328_000435"
     CDS             complement(71343..71708)
                     /locus_tag="IV328_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8936604.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(71871..72266)
                     /locus_tag="IV328_000436"
     CDS             complement(71871..72266)
                     /locus_tag="IV328_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8936605.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(72284..73246)
                     /locus_tag="IV328_000437"
     CDS             complement(72284..73246)
                     /locus_tag="IV328_000437"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8936606.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(73246..73458)
                     /locus_tag="IV328_000438"
     CDS             complement(73246..73458)
                     /locus_tag="IV328_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8936607.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(73479..74177)
                     /locus_tag="IV328_000439"
     CDS             complement(73479..74177)
                     /locus_tag="IV328_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8936608.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(74197..74739)
                     /locus_tag="IV328_000440"
     CDS             complement(74197..74739)
                     /locus_tag="IV328_000440"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8936609.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(74871..75875)
                     /locus_tag="IV328_000441"
     CDS             complement(74871..75875)
                     /locus_tag="IV328_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8936610.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(75872..76861)
                     /locus_tag="IV328_000442"
     CDS             complement(75872..76861)
                     /locus_tag="IV328_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8936611.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(76858..77664)
                     /locus_tag="IV328_000443"
     CDS             complement(76858..77664)
                     /locus_tag="IV328_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936612.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            77830..78786
                     /locus_tag="IV328_000444"
     CDS             77830..78786
                     /locus_tag="IV328_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8936613.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(78863..79381)
                     /locus_tag="IV328_000445"
     CDS             complement(78863..79381)
                     /locus_tag="IV328_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8936614.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(79469..79618)
                     /gene="rpmG"
                     /locus_tag="IV328_000446"
     CDS             complement(79469..79618)
                     /gene="rpmG"
                     /locus_tag="IV328_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8936615.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            79846..80292
                     /locus_tag="IV328_000447"
     CDS             79846..80292
                     /locus_tag="IV328_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8936616.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(80487..82382)
                     /gene="asnB"
                     /locus_tag="IV328_000448"
     CDS             complement(80487..82382)
                     /gene="asnB"
                     /locus_tag="IV328_000448"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8936617.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        23445 a        17607 c        14299 g        27304 t
ORIGIN      
        1 ctttgttgag tattaatcta tttatttctt ctgcaggcaa gttaattttc ataaccttat
       61 gggggattat ccaattaaaa atcggaaaaa ggtagcctcc ccaaaaagta tagtgatctg
      121 tgtcaatcag ataataacct gattttgatt ccagaataac atatctcaaa gccgaacgaa
      181 aatttttcat gaatatccat aatctcactg tttccacacc taattcctcc aattccaatt
      241 aaatggattt atagcatcac caattatgtt aactgcctga ccaacctcat tgataaaatt
      301 ggcaccacta tcccatgctt caacaatgct atatatctat caaacaaaaa atggaaaact
      361 aaatactcgc gcacataaat tttacattct gttttttata agactaataa taatcattga
      421 aaaaatttct tatcggtggc gcatattccc aattgattaa tgtgatccca tattctgcaa
      481 gtattttacg agcttcttct aagtcatagt gagaaaaatg cataaaacct gcaatacgat
      541 cacggactgt gagctgtggc ttgcttccac catcattcat aaaatatcca gcagagccac
      601 tacttaaagt gattgttaca tcttcacttt ctattattaa gaattcataa tgaaatgctt
      661 tcaactctcc actattattt aaaagtttta tactccaact tcgtctaggt tgagttgtca
      721 cattccctct aaaacttcct ataaattctg caatcaccgt atcttcaatc aaaattcttt
      781 cctcatctat ttcatattca agactaaacg gaaattctcc atatgtaatc tctggcaatg
      841 gtggtgctgt agttctccaa ataaatacaa taactgtgaa tagaaaaaat ccaacgatcg
      901 atataaatgt tatgagacct gctcgaagta aaacccgata taataaaaat gctgtatttc
      961 gctttgctat atctagaaaa ataaaaagta ctgcaatcat aatgatagta aaagttgtaa
     1021 aaaagcacat tttttgccct ccctttataa acatcgattc tcaaattcta aaccaaatct
     1081 tgatagttca agttcaggta taacgtatac caaccataca tcccaattac tatttatgaa
     1141 cttatgatta tcactgaata aatcactcca tccccttgtt aaaacttcta cattaattac
     1201 acctggttgt tttcgactag ctaatccaat gaacttatca accatcctta tccaccaacc
     1261 acgagaatct tgaccagaag taagaaaaac agttctcaag gttgatcgag cagttccttc
     1321 cagtttttaa ttaatctcca gttatagata aatacttgat cctatctata tcaggacttc
     1381 tatattaagc aaaagtttat gtataaaact aaaatcttca ttcagttgtc tccccgcttt
     1441 ttatacgatt gtattaaaaa acaatagaag cctttcaaca ttatgttact ccagtaaagt
     1501 tggaattata gtttgacgga agaaaagcaa gtatagcttt taaaaaaaac tgatacattc
     1561 gcatctcctg cttcatgtat cagttttttc tttggattaa actgcttctt cacgcagttt
     1621 ttccgcaaat tcatttattt tgcgaattcg gtgattaatt cccgattttg atattgcacc
     1681 tgagggaatc atttccccta attctttcaa gctgacctct ggatgttgaa gccgaagttc
     1741 tgcgatttcc tgaagttttt caggaagggc atgtagaccg acctttgctt cgatcaattc
     1801 gatgttttcg atttgtttag aagccgcatc aatcgtcttg ttcagatttg ctgtctcaca
     1861 attgactaga cgattgaccg aatttctcat atcccgtacg attcgaacat cttcaaatcg
     1921 gagcattgag ttggttgcac caatcaatgt taagaaatca gcaatttttt ctgctccttt
     1981 taaatacgaa atatagccat tgcgccgttc aagcgttcga gcatgtagac cgtaataatt
     2041 taacatatcg caaatgtctt ggttatgctg ttcataaata gaaaatattt ccaaatgata
     2101 acgactcgtt tccggattat tgactgaacc tgaggccata aaagcaccac gtaagtaaga
     2161 acgcattttt tgcgaatttc ccataatctc gtccgaaacg tgtccattga acatcatacc
     2221 atccatgata tcaagatcca tcaacacatc ttttgtgcct tgcttcaacc ggacgatata
     2281 gacattgttc ttttttaatt tcattttttt acgaacgagt agctcacttc gtacctgata
     2341 atgatctttc aaaagagaat agatacgtct ggcaattgca gcattttctg tttggacgtt
     2401 caaaataaac tgctggttca caatacctaa agagccgttc attcgcagca aagcagctaa
     2461 ttccgctttc gcatgttctc gatgaacttc caaaccagtt agttctttct tcacatctgc
     2521 agcaaatgac atcctattct cctcctttct ccaacaataa attgcttaat attttgaacc
     2581 gaaaactaaa cggaacaatt cttctactac tttgtctcca tcgtggaaga caccaccatc
     2641 tttcaattct aaaaagtcgg tggaaatcac tcgacatcct tcatttctca atccgttgaa
     2701 gtcgtgtgcg acttgtacta gatactcatc atagatctct gggtccatat aatcttcagg
     2761 cactttttcc gtattgacca atactgtatc gataaaaggc tgattcaaat gcttgtgtaa
     2821 aacacgtata tgatcggcat ctgtgaaatg ttccgtttcc cctttttggg tcatgatatt
     2881 acagatatag acggtttcag cagttgtctt gagcattgct tcgccgattt ctggtatcac
     2941 taaatttgga aggatactgg tgaataaact tccgggtccc aaaacgacca tgtcggcgtc
     3001 cataattgct gaaacgactt ttcgagccgc tttggcttca cttttatcat ttgtgttgca
     3061 gacatacaca cggtcaatgg ttttacggtt gatcgcgatt ttagattctc ctactgcagt
     3121 cgttccgtct ttaaaaacag catgcaaggt caaaggagtt tctgaagaag gataaacatg
     3181 tccatctaca tgcatcattt ttgctaataa ttgaatcgcc tcataagtgc ttccccgcat
     3241 ctcggaaact gcggcaatga tcagattacc gatcgtatga ttggctaaaa actgatcttc
     3301 ctttttgaaa cggtattgga aaatatcttc atagaattgt ggcatatccg ataacgccac
     3361 taagacattt ctcaagtcac caggaggagc catagcgatc gattctctga ttgcaccgct
     3421 gcttccacca tcatctgcaa cagtcactac tgctgtgata tctgctcctt gatttcgtaa
     3481 acttttcagg ataactggca gaccagtgcc accgccgacc actacgatct ttggttttct
     3541 tatccggtag gttttcattt tcacgaccgg ttcaccgtct ctttcctttt atccttgtcc
     3601 cgatgtgtca cattgacttt atagtttttc cctaaagcat ttgccaaacg ttcagtcaat
     3661 gccacagaac ggtgttgacc gcctgtacag ccgattgcaa ctgtcaagct gctttttcct
     3721 tctttaatgt atccaggcaa tacagtttcc agaagatcca aaaatttatg gtagaatgat
     3781 tgcgtttcat caaaggacat cacataatca tagactggct gatcttttcc ggtcaatggt
     3841 cttagttccg ggatataatg agggttgggc aaaaaacgca catccatcac aatatccgaa
     3901 tcgatcggca acccgtattt aaaaccaaat gatacaaatt cgatccgaaa tccttggtct
     3961 ccctttgatt tgaatgactc attgattttt tcacgtaatt gtcgtggtgt caatgttgtc
     4021 gtatcaatga tcacggaagc ttgggctttc aagtcgtcta gtatcgcccg ttctttacgt
     4081 atcccttcag tgatcaaacc gtccatcgcc attggatgtg ctcgtcttgt ttctttgtag
     4141 cgagccacta gttcttcgtc tgaagcatct aaaaataaga tagaagtgtc gatgaagttc
     4201 gtattttcga tttcgatcag catgttttgg atttcttcaa agaaggaacg agaacgcaaa
     4261 tccactccta aagccatttt tgtgacttta cctgattctt tgatcaattc ccagaatttt
     4321 ggaatcaatc gaggcggcat attatcgata caaaaatagc ccatatcttc aaaactttgg
     4381 atagctactg ttttccctgc accactcata cctgttatga tgactaattg caaattatct
     4441 gtcatattta cgaacccctc tcacattgtg cataataaaa ttatatcatc ccgggcgtat
     4501 cattaccagt tttctttttc ttttacaaaa agatttggac caaatatctt ttaggatatt
     4561 tgccccaaat ccaatttttt tacttttccg atgctgtatc caatgcctcg cttctttcag
     4621 ctaatgcacg ttcgatcgtt cgatccgcta aaatagattg agcatcgatc actgggtaat
     4681 gattatcttc agcaaattca tgcatcaaag ataattctgt gcaacctagg atgacttttt
     4741 cacagtttag gcgctctacc gcttcctcta agatttcgta atagagctct tgattcaaat
     4801 aatccgattc cttgatctca tgatagatca ggtaattgat tttttcttga agcgcagtat
     4861 caggaatcac tgtttcgaaa ccaagatttt tgacttcgcg ttcatagatt ccagctttca
     4921 tacttccttc tgttcctaga atcgctactc tccctgtcgt atgctgacgg acaagttcat
     4981 ttgctgcttc tcttggcata tgcaagatag gaatatccgt tgccgcttgt aactcttcaa
     5041 aaaagtagtg tgcggtgtta caagtcaaca caatgaagtt tggtcgcaat aaattttgtt
     5101 tctcaatatc atccagcaga aacggcattg gattttcttc tgatcgatct aaaatataag
     5161 ctgtacgatc aggaactgtt gcatgattga ataagacata attcaaatat tcttgatctt
     5221 ttgtagcttt cgttcggtgg ttgatcaagc gaacaaaact ttctgtcgcc atcgtgccca
     5281 ttccgcctaa aatactgaaa aaattctcca tgaactagcc ttctttcaca tgaaaatact
     5341 ttttgaatct tggaatatag ttatggaaca tatatttcat caacaaccag cgcttgattg
     5401 atcggtcttt ttcataaaat acagtagtac cgtaacgatt ttctttgatc atttgctcag
     5461 ctagtttttt gtcttcattt tcccgagcat attctaaaaa gatttttttg ggcacaccca
     5521 tccataatct tgctttatct gattgattcg ttccataggt cgtttcaaca aagggtttgt
     5581 tgaacactcg atcttctgtg acaaaacgag ccagattcaa gccgttcaat gtcacaaaga
     5641 aactgctacg accttgtctc aaattgattt caaataattt atattctcca tctctaggat
     5701 catacttcat atcgaaattg gcaaagcctg tatattcgat tttttccaaa aatgccttaa
     5761 tggtttgata gattttttcg ttataatctg gcatgatcac aacgtaatta ccaatcgatg
     5821 ctggtgtagg atcttctaat agtggatgac caaggcacat catccgaact tgatgatctt
     5881 catctacata cgcatttagc acacgcatat tactatcatc acccgggata aaatcttgta
     5941 caatcatttc acttttataa ccggcttcgt atatccggcc taaaattaaa tcgaactctt
     6001 ctctagtctc taaaataaaa gctttttttc ttccttcaaa ttggacagaa agatactcca
     6061 cactgtttgc aggtttcaaa gcaactggga attcaaaagg caattcctgt tccaaatgtc
     6121 catttactaa catttcttct ctgacgatca atgttttagg atacggaaga tcatattctt
     6181 cacaaacttc ataaaagctt actttattga tcagcctttc aaataaagaa taatcaatat
     6241 atggacagat aaaatattct gacagctctt gtttatgttg ggagatcaat tctgcatatc
     6301 catcaccaca agcaatcaaa agatattttt tactcttatc agaatatctt tcttttgcca
     6361 aacgaagcat cgtctcgata aatacagggt ctttatcaaa tcctggaata acttctacat
     6421 tgacaatttt actgtagcgg gtaggggcta actgatccga agcatatgct tggcagattt
     6481 tgccgtatgc ttcattaaaa gaacgtgcca taccataaac gttcatgtcg cttcctaata
     6541 atatagggat aaattcttca ttttcaatac tgttcatcat tatctctcac ttactcataa
     6601 tctcttgttt ttcttttcca actacacaag gaatatcata ccataaagaa aaaagcaatc
     6661 aaaggaaagt cctccgattg ctcaattctt ttttgaacgg attgaactta taggaagttt
     6721 ttatctatac caatcagtgt agaaagaact accatgtaat gattctagaa cactgatatt
     6781 aataaaacaa gcaaaaattc atttttttat gtttttttaa ctatctcagc tatctatcct
     6841 ctttatacca ttattttttt caaatagtga cctgtataac tttcttttac gttaatgatt
     6901 tcttctggtg ttccagtcgc aaggatcgtt cctccacctt ctccaccttc tggtcctaaa
     6961 tcaatgatat aatctgctgt tttgattaca tctagattgt gttcaatcac taaaactgtg
     7021 ttaccagcat ctactaatct ttgtaataca tgcaacaagc gggcgatgtc atcgctatga
     7081 agtcctgtcg ttggttcatc tagtatatag aaattctttc cattagagat tttgtgcaat
     7141 tcactggcaa gtttcatccg ttgtgcctca ccaccggaca atgtcgttgc tggttgcccc
     7201 atcgtcacat aacctaagcc aacatcaaca atcgtttgca gtttgcgatg aatctttgga
     7261 atgtgcttga agaattctac agcatcctct actgtcattt ccaaaatatc agcaatgctt
     7321 tttcctttgt aatgcacttc taatgtttca gaattatatc gtttaccatg acatacttca
     7381 caaggaacat agacatcagg caaaaagtgc atttcgatct taataattcc atccccgcga
     7441 caagcttcac aacgaccgcc ttttacgttg aaactaaaac gccctttctt ataaccccgc
     7501 atttttgcct cgttcgtttg agcaaataac ccacggatat catcaaatac acttgtataa
     7561 gtcgctggat tacttctcgg cgtccggccg attgggcttt gatcgatatc gatgatcttt
     7621 tcgatacttt cgtagccgga aattgtcttg aatttacctg gcttagcaga attcttattt
     7681 aatttttgcg ctaacgattt ctttaagata ctattgacca atgtactctt tccagaacca
     7741 gacacacctg tgactgctac aaattctcct aatggaaatt caacactaat atttttcaga
     7801 ttgttttctg aagcaccagt gatcttgatt gctttcccat tacctttacg gcgttctttt
     7861 ggtacaggga ttgatttttt ccctgaaaga tatttccctg tcaaagaatt ttcatcatta
     7921 gctacttctt caggagtgcc tgcagccacg atttgtccgc cttgttctcc ggccccaggt
     7981 ccgacgtcaa tcaaataatc tgctgcccac atcgtatctt catcatgttc gactacaacg
     8041 agggtgttgc caagatcacg cattttcttt aatgagccaa tcagtcgatc gttgtcgcgt
     8101 tgatgcaatc cgatcgatgg ttcatctaaa atatacaaaa ctccagaaag attcgatcca
     8161 atttgtgtag ccaatctgat tcgctgcgct tctcccccag ataatgttcc ggaagcgcga
     8221 ctcaaagtta gatagtctag tcccacattt tccaagaatg aaagtctgtc atttacttct
     8281 tttaaaatcg gtttggcaat tactttctct tgttcagata acgttaagtt tgagaagaaa
     8341 tctactgctt tttgaataga caactcattt gtttgcccga tattcgttcc attgatttga
     8401 acagatagag cttggggatt caatcgataa cctttacatg tctgacaagt taattctgtc
     8461 atataaagac gcatttgctc acgcgtgaaa tcactgttcg tatcatgata acgacgctcg
     8521 atattcttca ataccccttc gaaagtcgtt tctacatcgc gaacgccacc aaaatcattt
     8581 tcataatgga aatggaaaag atcatctgtc ccattcaaga tgatctcctg atgctcttcc
     8641 ggtaattctt cgaatggtgt atctaaatcg ataccgaaac tttcacaagc ctgcgctaac
     8701 atttgaggat aatactgaga gctgattgga ttccatggaa cgatagcacc ttgactaagt
     8761 gtcttcgttc tatctggaat cactaaatct tgatcgactt ctaatttgac acctaaacca
     8821 tcacagtctg gacaagctcc aaaaggcgca ttgaatgaga acagtcttgg ttctagttcg
     8881 ccaacagtaa atccgcaata aggacaggca tagtgttcgc taaacaacat ttcttcttca
     8941 ccgatcacgt caaccagagc atagccgtct gctaatcgca gggccgcctc taaagaatca
     9001 aataggcgag aacgaatccc ttctttcaca atgatccggt cgatgacgat cgcgatatca
     9061 tgttttttgt ttttctccag ctcaggcact tcacttaaat catagatctc accatcgact
     9121 cgaacgcgga cgtatccctc tttttggatt cgttcgaata tttttttatg ttgtcctttt
     9181 ttctttacca cgatcggtgc caacacttgg atctttgtgc gctcaggcaa agccattact
     9241 tgatctgcca tttgatctac tgattgactg gtaatctcga tatgatcgtt gggacaaatc
     9301 ggatgtccta cacgtgcaaa caacaagcgt aaatagtcat tgatttccgt cacagtacct
     9361 actgtcgacc gaggattttt acttgttgtt ttctgatcaa tagaaatcgc cggactcaat
     9421 ccgtcgatgc tatctacgtc cggcttatcc atttgtccta aaaactgacg agcataagca
     9481 gaaaggctct ctacgtatct ccgctgccct tcagcgtaca gcgtatcaaa tgctagggaa
     9541 ctttttccag aaccggacaa acctgtaacg accacgaatt tatctcgtgg aatcgttaca
     9601 tctatatttt tcaagttatg ggcacgtgca ccatgaatcg taattttatc gttagccatg
     9661 ttgtcctcct actttgaagc ttttaattct aaaatcatat ctcgcacatt cgcggctgtt
     9721 tcaaaatcca aagcttttgc cgcatctttc atttcttttt ctaatttcat caataacgtg
     9781 tctttttctt ccttcgtcat ttcttcgtac gagacttgta ccgcttcttt tgtatcatct
     9841 tccgattctt tcgtaataga aatcaaatct cggatttctt taatgatcgt ttttggaacg
     9901 atcccatgtt cttcattgta cttctgctga atcgtacgac gtcttgacgt ttcatccatc
     9961 gctagacgca tagaatctgt tactttgtct gcgtacatga ttactttacc ttcttcattc
    10021 cgagcagctc gtccaatcgt ttggactaag gaacgttcac tacgtaaaaa gccttcttta
    10081 tccgcatcca agatagcaac aagggaaact tcaggtacgt ctaatccttc acgaagcaaa
    10141 ttgattccaa ctaacacatc aaattcacct aatcgaagat ctcgaatgat ttctgttcgt
    10201 tccagcgttt tgatatcact atgcaaatat ttgactttga tccctaattc tttgaaataa
    10261 tctgtgaggt cttctgccat ttttttcgtc aatgtcgtta caaacacccg ctggtctttt
    10321 tcgactcgtt catttatttc gcctactaga tcatcgattt gtcccatgat tggacgaact
    10381 tcgatcactg ggtcaagcag ccctgtcgga cggatgattt gttggataac ggtatctgtt
    10441 tgttcatgtt catatggtcc tggcgtcgca gagacataaa tgatctgatt gacatgtttt
    10501 tcaaactctt ctaaccgaag tggccgattg tctaaagcag atggcaaacg gaaaccgtaa
    10561 tctactagca tctgttttct cgctcggtca ccattataca ttcctctgat ttgaggcatg
    10621 gttacgtgcg attcgtccgc cacgatcaaa aaatcttcag gaaagaaatc caatagcgta
    10681 taaggcggtt caccttcttt acgtccgtcc atatgccgag aataattttc aattcccgac
    10741 gtgtagccca tttctaacat catttcaata tcgtaattgg ttcgctgttc taaacgttga
    10801 gcttccagaa gtttgttttc atttcgcaag acagttaaac gctgttctaa ttccgctttg
    10861 atattcgcta cggcatgctc catatgttca tcattcgtca caaagtgagt cgccgggaag
    10921 attgctacgt gttccgtttc tcctaaaact tcacctgtca gtgcattgac ttctcggata
    10981 cgttcgacct catctccaaa aaactccaca cgtaaggcac gttcatctct tgaagccgga
    11041 aaaatctcca cgacatcacc gcgcactcga aaacgaccgc gctgaaaatc tatatcattt
    11101 ctttcgaatt ggatactgac taagtcacga atcaactgat tgcgatctag ttctgctccc
    11161 tgtcgaatcg aaactacttg tttttggtac tcaaaaggcg agcctaatcc aaaaatacat
    11221 gaaacggatg caatgacgat cacatcattt ctttctaata atgaactagt agccgaatgt
    11281 cgtaatttgt cgatctcatc atttacactt gaatcttttt cgatatacgt atcgcttgaa
    11341 ggcacataag cttcaggctg ataataatca taataagaaa caaaatattc aacagcattg
    11401 ttagggaaaa attccttgaa ttccccgtac agctgaccag ccaatgtttt gttatgggca
    11461 ataatcagtg ttggtttatt cactttctca ataagattgg aaatcgtata tgttttccct
    11521 gttccagtcg cacctaataa gatttgtgct tttttcccgc cgactacgcc atccactaat
    11581 tggttaatcg cttcaggctg atcaccagcc ggttggtatt tcgagacaag ctcaaagtgt
    11641 cgggaggtat ctctctcaat cattcttcgt cctccttttc attcattcct tttcatttta
    11701 cgaccaataa aaagaaaagg aaagcaaagg cattccttac taaaaaatta taccacaccg
    11761 aacatacttt cgccactttt ttatcttatt atacatgtta tgttttataa cataaaatgt
    11821 tagaaactcg catttttttc attttttcga gcttacagtc ttgtctgatt cagcactttt
    11881 cgcctataat aataaaatat tggaaattta ggaggttgat tatgaaaaga aagcatttat
    11941 ttcactcatt tgttttctta atgacaggac ttgtgacatt tgcgtttggt catgttgcac
    12001 aagctgacaa cactacaacg gcagagtcaa gcacaagcga gacaatgcaa aaaatagagc
    12061 ccaaaaaaga cgtatacgta attgccagtg actctgcttt tgctccattt gaatttcaaa
    12121 acgcaaaggg cgagtacgaa ggcatcgatg taaaattgat ggaaaacatc gccaaactgc
    12181 aaggatttaa catccgaatg gactaccgtg gtttctctgc tgctttgcaa gctttggaat
    12241 caggacaagc agacggaatg attgccggaa tgactgtaac agatgaacga aagaaaagct
    12301 atgatttttc tgattcttac tttgatagcg ggatccaatt agctgtcaaa aaaggagata
    12361 cttcgatcaa gtcttatgaa gatttgaaag gaaaaacagt cggagcaaaa gttggtaccg
    12421 aaagtgctga atttttaagc gaccatgaat ctgaatatgg ctattctatc aaatattttg
    12481 atgctgctga tcaattgtac gacgggttaa aagttggcgc aatcgatgca atgatggatg
    12541 attatccggt aatcggttat gcgatcaaac aaggacaaga attggaaaca ccgatcaaaa
    12601 aagaatctgg cggaaaatat gcatttgccg ttaaaaaagg acaaaatcct gagcttctta
    12661 atatgttcaa tgaaggattg aaagaattaa aaagaacagg cgaatacgat aagattatta
    12721 aagaatatgt agcggatggt tcagaaactt ccagtgagtc ttccactgct gatgaatcaa
    12781 catttcttgg acttatccaa aacaactatc aagcattgtt gaaaggactt tggagaacaa
    12841 ttctcctaac tttgatctcc tttgctttgg ctatagtaat cggtgtgatt tttggattat
    12901 tcagtgtagc accaatacgc gctcttcgca caattgctag tatttatgtc gatatcatcc
    12961 gaggaattcc aatgatggtt cttgcgttct ttattttctt tgggttgcct ggtatcgttg
    13021 gatttacgat tcctgacttc cttgctggtg ttatcacctt gactcttaac gcaagtgctt
    13081 atatcgcaga gattgttcga ggcgggatca acgccgttcc tgtcggacaa atggaagctt
    13141 ccagaagttt aggtctttct tataatcgga caatgcaaaa aatcattttg ccgcaagcga
    13201 tacgcatcat gatcccatca ttcgtcaatc agttcgttat ctcattaaaa gacacaacga
    13261 tcatttccgc aatcggtgta gtcgaattgc tgcaaactgg taagatcatt gttgcacgta
    13321 caacacaaag tacgtatgtt tacttgatca ttgcgatcat gtacttgatt ttgatcacag
    13381 cattgacaaa attagcaaaa gttttagaaa agaaggtgaa gtaagatggc agaaaaaatc
    13441 ttagttgagc atttagtcaa aaaatacggc gataacacag tattaaacga catcaatgta
    13501 tccatcaacg aaggagatgt cgtctgtatc attggccctt caggttcagg aaaaagtaca
    13561 ttcttgcgtt gcttgaatcg tttagaagaa ccttctgacg gtgaaattat tattgacggc
    13621 gcccacttga tggataaaag cacagatatc aacttggtga gacaacatat cggaatggta
    13681 ttccaacact ttaacctatt ccctcatctt tctattttgg aaaacatcat tttagcaccg
    13741 atggacctga agaaagaatc tagagaagaa gcagagaaaa aagcaatcaa attactgcag
    13801 acagttggtc tagaagataa aaaagatatg tatcctgaaa tgctttctgg tggacaaaaa
    13861 cagcgtgtgg ctattgctcg tgctctagcg atgaaccctg acatcatgct ttttgatgag
    13921 cctacttctg cactcgatcc tgaaatggtc ggtgatgtct tgaacgtcat gaaaaagtta
    13981 gcagaacaag gtatgacgat ggttatcgtt acgcacgaaa tggggttcgc taaagaagtg
    14041 gccaatcgcg tcatgttcat tgacgacgga aactttttag aagatggaac gcctcaacaa
    14101 atctttgaaa atccgcaaaa tgaacggaca aaagatttct tgaataaagt attaaatatt
    14161 taataaaaac caaaaatgca cggtcgccat gaagggaccg tgtattttta tcttcttatt
    14221 ttctcaccta atcgtatcag ccgcatcgct tgttttcctg cacaaacaag caatcgctct
    14281 acctgttcca tcgcataaga aagtaccatc ggttcattga tgatatcaag tacatagtcg
    14341 ataccaccct cacttaattg agcttgattt ttcttctcca tattatgtag ttgtgggaca
    14401 actttctgac aatcagcaat tgtgtcaaga acggatcaat cctttattgg agttgctcga
    14461 aagggtcttc tcttattacg gacaggcttt gctcactgta catagacaac aaatcattat
    14521 cctagtcaat cggatttcca gagcctcatt gctatcattg cttgataaaa tccagacaaa
    14581 gtttaataaa atgtatcagt tacagctaaa ctttggaatt gggtcgcttt gttatacaga
    14641 gcaagagaca ccgcaatctt tccttcatgc caaacaagtt tgcgaatgga tagctttcca
    14701 tcaatctgtg aacgagatcc gcttttttga agatttagac ctcgggatcg ttttaccagc
    14761 aatcccgtca gatcaacgaa cattatatgt aaaacgtatt ttgaaaagcc tgacagaaga
    14821 agaagtacat ttgttcaaga agactttggc atgcttcagt aaaaataacg gaagcatcaa
    14881 aaattgctca gaagaattat ttattcataa aaacaccttg caatatcgat tgaacaagtt
    14941 ccattctctc actggttata cccctagaaa ctatgacgat tatcatattc taaagctggc
    15001 ttttttgttg gtacaaacat gacaactaca aaaaaaccag gcgaacatat gattgttcgt
    15061 ctggttttta attattaatg acttagcgtt tttactagaa aaactacttc cgctttttcc
    15121 cgccaaaagc atctttaaac ttatcgaaga aaccgtcttc ttgctgttct tcaactttga
    15181 tgccgccagc ttcagcgaat gcacgtaatg cttctctttg ttgttcattt agatttttcg
    15241 gtgtgatcag ttttactttg acatgttggt cgcctgttgc accaccacgt aacttcggag
    15301 cgccttttcc acgtaaacgg aagttcgtac ctgtctgtgt tccagcaggg atcttcaatt
    15361 tcacatctcc atggacagtt ggtacttgga cttcatcccc caaagctgct tgtacaaaac
    15421 ttaaaggcaa ttcatagtaa atttcagaac cttcacgatc aaagatatca ctttcttcta
    15481 cacggaagac cacatacaaa tcaccaaatg gtccgccgtt ttctccagct tctccttgac
    15541 cagctaaacg catttgttgt ccttcttcta caccagctgg tacattcact ttaactgaat
    15601 gtgctttctt ctcatgtcct gagccatgac aggttggaca aggttctttg atttctttcc
    15661 ctgtcccatg acaaacatca caagtttgac ggctcatcac tcgtccaagt ggtgtttggc
    15721 gttctacatt aatcgtacct gcaccatgac atttatgaca agtagttggt tgtgtgcctg
    15781 gttttgcacc gttcccacca caagtatgac agatttcttc ccgattatac ttgatttctt
    15841 tttctacacc aaaaatcgct tcttcaaaag acaagtcgac cgtatattgc aaatcagcac
    15901 cttgacgagg cgcatttgga tcgactgacc gaccgcctcc tccaaagaaa gagtcgaaga
    15961 tatcttcaaa accaccgaag ccgccaccag agaagccgcc gaatccacca aatccgccac
    16021 cagctccacc agcaccgtaa tttggatctg tgcctgcatg cccgtattgg tcatatgcag
    16081 cgcgtttttg cgggtcactt aaaatctcat atgcttctga tacttctttg aatttttctt
    16141 ccgcatcagg ttctttattg atgtcaggat ggtatttttt cgaaagctta cgataagctt
    16201 tctttatttc atcttctgat gctccttttg agagccctaa gacttcataa taatcacgtt
    16261 ttgttgccat aggctttcct cctattggtt ctcatcattt tcacgtcgct tgttatcata
    16321 tcatattttg tttgaaacaa aaaccgatac ttcaaactat ttttcaaaag aaaaagccaa
    16381 agtagcaact ttggccttct ctcgaaattc agtgaactga ttatttatcg tcgccatcga
    16441 cttcttcaaa atcagcatca acgacatcat ctgctccgcc ttgagctgct tctgggtttt
    16501 cttgagcttg ttgttgtgcc gcttgttcgt atagtttaac agtcaagttt tgaacgattt
    16561 cgttcaatgc atcgcgttta gttttcattt cttcgatgtt gtttgcttca acagcggctt
    16621 tcaattcatc gcgagctgtt tctgcttttt tcacttcttc ttcgtccact ttgccttcta
    16681 attctttcaa tgttttgtcc acagagaata gcaatgcatc tacatcatta cgtaggtcaa
    16741 cttcctcttt acgagcttta tctgcttctg catttgcttc tgcatctttt accatacgtt
    16801 cgatttcttc gtctgttaga cctgaagaag atttgatcgt gattttttgt tctttttgtg
    16861 tacctagatc tttcgcagat acatttacaa tcccgttttt atcaatatcg aatgttactt
    16921 cgatttgtgg aataccacga ggagctgctg gaatatctgt caattggaat cttcctagag
    16981 ttttgttgtc agcagccatt gggcgttcac cttgtaatac atggatgtct actgctggtt
    17041 gattatctgc cgcagttgag aatacttgtg atttacttgt tggaatcgtt gtattgcggt
    17101 caatcaattt agtgaacact ccacccattg tttcgatacc taatgacaat ggtgtaacgt
    17161 caagtaaaac aacatctttc acatcaccag tgatcacgcc accttggata gcagcaccca
    17221 tagcaactac ttcatcaggg ttaactgatt tgtttggttc ttttcctgtt tctttacgta
    17281 ctgcttctac aactgcaggg atacgagtag atccaccgac taaaataact tcgtcgattt
    17341 ctgattgtga taaacctgca tcttttagcg cttgacgtac tggtactttt gtacgttcta
    17401 ctaagtcagc tgttaattca tcaaattttg cacgtgtcaa tgtcatttcc aaatgcaatg
    17461 gtccagattc tcctgctgtg ataaatggca agctgatttg tgtgcttgat acgccagata
    17521 aatctttttt cgctttttca gcagcgtctt tcaagcgttg taaagccatt ttgtcttgag
    17581 acaagtcaat gctgttttct tttttgaatt ctgctaccat gtagtcgatg attttgttat
    17641 caaagtcgtc cccacctaga tggttgtcgc cagctgttga caatacatca aagacaccgt
    17701 ctcctagttc aaggatagat acgtcaaaag taccgccacc aaggtcgaat actaagattt
    17761 tttcgtcgcg atctgtttta tctaaaccat aggcaagagc agctgcagtt ggttcattga
    17821 cgatacgttc tacttctaga ccagcgatct taccagcatc ttttgttgct tgacgttgtg
    17881 catcattaaa gtaagctggc acagtgataa ctgctttttc gactttttca cctaaatagt
    17941 cttctgcgaa tcctttgata tattgaagga tcattgctga gatttcttgt ggtgtatatg
    18001 atttaccttc tacatctact ttgtaacctg cttcaccgat atggcgtttg attgatgaaa
    18061 tcgtatttgg gtttgtcaca gcttgacgtt ttgcaacttc ccctacttgg atttctccat
    18121 ttttgaatga tacaacagaa ggtgttgtac ggtttccttc tgggttagcg atgatttttg
    18181 cttcgtttcc ttccaatact gctactgcag agttagtcgt acctaagtca ataccgataa
    18241 ttttactcat aattaacgtc tccttcttta attgaaagtg taagttttat atccgcacaa
    18301 ttattgtgca acaatgacca tggttggtct taacacgcga tcatgtaatt tgtagccttc
    18361 ttgtagtact tctacaatcg tatcggctgg tgtatcttca gtcgctggaa cagtttgtac
    18421 tgcttgatga agattaggat caaaggcttc tcctttagca gggatttttt caattccttc
    18481 ttcttccagt gcgttacgca aactttccaa gaccatttcc acgccttttt tcaaacctgc
    18541 cccttgatca tctgacactt ctgtggctaa tgcacgttcc aggttgtcaa ttgatggaag
    18601 taattttttg gctaaatctt gtgaacggta tttttgtaat tgctcacgct cattttttcc
    18661 tcgatttgcc atatttgcga tttcagcacg agcccgcaag tatttatctt ccatttcttc
    18721 taactcagct tttaatttcg ctgtttcgaa ttcttccgct tcagcttctg aaatacccga
    18781 atcttctgca gcttcgacat cgatttccgg ttctggctga gcatctgtca tttctttatc
    18841 tagttcttct tgattttctt tcacgataca gtcttccttt cattattcag tcagctgttt
    18901 gattgctctt actaagaaag agagaacgat cagctgcaaa tagcttcgtt attcacatta
    18961 cgagtttgac aagtcaagcg aacggtagta atcagcaagt ttagctgcta gttctcttcg
    19021 ataaacatcg actagaccga acatccgaga ataaggcata ctagtaggcc ctaacaaggc
    19081 tattgtacct cttccatgac catctatctc gtaacttgct tgaatcatac tcatattctg
    19141 caataaatca ttgcctaatt cagcaccaat cttgatatga atattgctat ccatcggagt
    19201 gatcatttgt gtaagtccgt ctgagtcttt cataaaagaa aacatcgatt tgaactgatc
    19261 aagatcttga cggggctcat aatccaagag attcatttgt ccactgacat aaaccttatc
    19321 ttcaaaaaca tgaccaagaa cggtgtcaaa taactgagac atcccttctg tcgtttggaa
    19381 atatttatga agaatcatcg gaatctccgt acgaagttta tggtacaccg tcatcagagg
    19441 atctccgact agacggtcgt ttacaatctg taccattttt tctagttcct gacttcccat
    19501 atattcggga agagtgaaca cttggctttc tacattcccc atatctgtta cgacgatagc
    19561 aataatctgt cgattattca agggtacgat acgaaagcca gtcagtcggc ggtcttttat
    19621 ttcaggtccc aatgaaaaag tcgtatagct tgtcagctca gataatatct cagcagattg
    19681 cttaataatc tcattgattt catgaaattc tctgcctaga gactgacgaa tcagcagcaa
    19741 atcatcttct gctacgcgag caggttttaa caaatgatcc acgtaataac gatagcctaa
    19801 agcagatgga atccgcccag aagaagagtg agtcttcaac agcaatcctt catcttctaa
    19861 tgctttcata tcattgcgaa tcgttgctga gctggccttg actccctcag acatcaatgt
    19921 tttggaacca acgggcacgc cactactcgt atagtgttgg atgatcagac gtaaaatgtc
    19981 gttttgtcgc tttgttaaca tcttcctcac ctctcattag cactcatata ttctaagtgc
    20041 taatccactt ataaatatag caagaagccc ttctcctgtc aagagaaaga ctccattttt
    20101 tagcactcta aacacaagag tgctaattct aaataattga tgtaaatcca gttatatcaa
    20161 cgtttcaata gtcctttatt gtatttttga acttttttta tttaaattca gataaaaaac
    20221 aaatattctt taaactattt ttcaaacaaa aagcgctcaa acacattgtt ccccaaaaag
    20281 gtccctcttg gtgtcaaata aacacggtct tcttcattaa caagtaaccc ttcttgctct
    20341 aattccctta gcacatcacc gtacaccgat tcaattgttt tttgatagcg atcatagaaa
    20401 tgttttttag taatccctgt tttttttctt agtcctaaga acatttcttc ttctatttga
    20461 ttttttcgtg tcaatttttc ttcttccaat accgggcgtt catttgctcg taatgggcgt
    20521 aaatagtgtt ggattggtcc tttgttgcgg taacgaatat ctccaagata accgctagca
    20581 ccagctccaa acccaaaata gtggtcattg ttccagtaca tgaggttatg ttgagattca
    20641 tgtccggaga gcccaaagtt acttatttca tattggtgac gtccatgctt ttccattgct
    20701 tcaatcgtct cttcaaacat tctggtttcc gtttcctgat caggcagttc gagtcttcct
    20761 tgtcgtaccc agttcatgaa cattgttttg ttttcgagga tcaatgagta caaagaatag
    20821 tgaggcagat ccaaagccaa agctttttcc aaagtatcca gatatccttc tagtgtttgt
    20881 cccggtaatg cataaatcaa atcgatgctt acattggtaa aattttctgc ttctaaaaat
    20941 tgcatagttt gatacacatc ttctgcggtg tgtttacgtc caattttttt caataagcga
    21001 ttatcaaatg tctgcactcc catggataaa cgattgacgc catagttttt cattacttga
    21061 agtttttcac gggtcaaatc acctggattc gcttctacag taaactcatt accattttga
    21121 aaaggcagga tctctctagc acctgataac agtcgatcaa gttgtttagc agataaagaa
    21181 gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg ctgtcggatg actttctagc
    21241 attattttca tttcattcag cagcatttcc acatactcat ctactggctg cccttctaaa
    21301 aaaactttat taaaatcaca gtaatagcag atatgctcgc aaaatgggat atgaatgtac
    21361 gcagaaacct tgttttctgc gggatttatt gtttctatca ttgaaattct ttctccttac
    21421 gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc gttcaagcac caactcattg
    21481 aaataagccg catatttaga atcaaggaaa cctagctcta aaaatgcttg cttattgtca
    21541 tcggagctac acggtgcttt tacagcctct cgataaacgg taatcgcaaa gctgaccttc
    21601 ggcattaagt caaaatctgc aaaaatatga aaagctattt tcacagattc ctccttaagt
    21661 gctcagacca caacgctttg cttacaacct ctcatatacg catactaaac gtatttggcg
    21721 aacaaatcca ctaaaaaatc caagaatcga cttcttggat gattttttct ttcattaaac
    21781 tggttaccta gttatatatc tctagggttg ttttcatgtc tgagtgcccg atatgattta
    21841 tttttcgttt ctataattac tttttgcctt ccagtttctt ttaatattgt actcaagcaa
    21901 gtgggaattg gttcttttgt catccatgta tgcaaaatgt tataatgctg gtaaacaaaa
    21961 atgattacct gtcattttta tttagttcgt atttaattaa gttgactatc tgtatactct
    22021 gtctaaaact taagaattgt tgttttcgct gtcgctaccc aattttaatg aatgtctgta
    22081 ggttaaaact gttgtccttg ttatagatag taaaggagag cttgaaaatg aaaactgcga
    22141 ttacgtttct attagaggaa tatgccgatt gggaaggtgc ttatattact agcacactaa
    22201 accaaaatga gaaatggcaa aacaaaactg cttctatcca atcagaggtc acttccattg
    22261 gaggattaaa aacaaaaatc gattacttaa tcgaacaaat acctgaaaaa gttgaccttt
    22321 ttattatgat cggtggcaat tcatgggaca taaaaaatca tcatttatat caaataatcg
    22381 ataaattgtt aaaaacagac gtaccagtag ctgctatttg tggggcagtt gattatttag
    22441 ctcagagcgg attattgcaa ggttacaaac acaccggaaa tgcgcaattt ccttggttag
    22501 aatcggaaaa ttatcagaat aaagaagatt ttatagaaca acaagctgtg aaagatcgta
    22561 atttgattac tgcgaatgga acagctccta tagaattcac agacttgatt cttcaagcag
    22621 tccattttga aacagaagaa ataagagaaa aaacgattcg tctatacaaa cttggattct
    22681 atgattacta taaaaagtac ggagatcctt tctcctaatc attctcttca attaaatact
    22741 ccttatcaat agtgtgacaa gtaattttac tgatgattat ttgtcacact ttttctgaca
    22801 tttatcaaag aagatacact ctatttatat tcatagacaa ggttttacgg attcaaaaat
    22861 tcaggattat gctatactgg tattaaaaaa ataatgaggt attttatatg aaaaagttag
    22921 gttttatttt attgttggtc tctttattcc ttgtaggatg tacgtctcct agtaaagaaa
    22981 atagtgatcc atctgcgacc acagacaaaa gttcaatcag acaaaccagt tcagctgaaa
    23041 aatcaagtac tcagaaatca gcttctactg aaacgaatac atcttcaaca agcagtaaag
    23101 tctcagagcc caatacttct tctacatcta gtagtacagt tgagacaaat atttcttctt
    23161 caacaataga cacaacctcc tcacagtctt ctcagtcaat agatccgtta gcaggatatt
    23221 ctgatctaca aattgagtac gcacgtgttt ggctagctat tagaggtaat acttttagag
    23281 atacatcggg tgatttcgaa ttacatgtta ttcattatcc tgcaggaaca cctatcgccc
    23341 cttatgatgc agggagtgca gtctatccga aagatgtggt tatgctcact gggaaataca
    23401 gttaccaggg agttttagtg tattcaagta accatgacgg gacaatcact agttatccag
    23461 ttccttcaca ttggcaaatg cctgcagatc aaacatctta tccagcgttc attagagaaa
    23521 caactcaaga aatcattgat aatgcatctg tagtagctat tcctacagga aatcccaatg
    23581 acgtgaaaca gttaatcgat gtgacagtta tagataatta gactttctaa tatgttatac
    23641 aaaaagaagc tgacagcatt ttgtcagctt ctttcacttt cttttctatt tagatttatt
    23701 ggtctttgat agactccaag gcaggaaaaa attttttaaa taaacgtttg caaaacagtc
    23761 gcttttctat cttctaccgc tacttctgca taggcaccat taatcaatgt cagttgtggc
    23821 ggtacatgac cacaatcaat atcatacaag atcggtagtt ttaattcctt tgacagatct
    23881 tcgtatacat ccttcatttt ataatctaac aaatcctctg ccgcacttct accgaacatg
    23941 atccccgagc tgccatcaaa ccagcctgcc agtttcatct gaacaagtga tcttcttaaa
    24001 tcaactgacg atagctcaca gttttctaga aaccacaaga tcggctttcc atcattatac
    24061 tttcttttaa aagattggac atctccataa ggagtaccga ttaaatgcct gatcgtatca
    24121 atacaaccac ctaataatcg tcccttcatc cttactgcat ctccgctgac tgattgccaa
    24181 tttgttggtt cagtgagatg gaaaacagaa gatgatgaat gctcatgggg ccacactttt
    24241 tgatactttt cagaagattg ctgtagaatc gattctcctt ctcttgtttt cagtacactt
    24301 tcccacattg ctgtcgtttc atccaagtac tctcctctta aatcaataag gttagttcca
    24361 tgtgcagtgg ctatccctgt ttttaatgtg acagcaagta gcaacgtact aatatctgaa
    24421 taacctacta tccattttgg ttgtagagca tcaaaatcga caaaatcgag aatctcaatc
    24481 aataattctc ctccccaagt cggaaagatc atttggatat tttcatcttg catcatttga
    24541 ttgaattcat ctgctcgttt tcttgctgga cctgattttt ccttatcttg cgtccaaagc
    24601 gtctttccag taactacttg gaaagatttt tcttccattc tctttaaagc tgattcgagt
    24661 aacgaatggt gttcctctga tacgcctgag gatggtgcag taaccccaat cgttgcattt
    24721 ctttctaata atggataact tatcatgttg cttcacctcc aagtatgcac tattttactt
    24781 ggttaattgt ttttttcgtt tgccggcatc aaaaatatcg gcatctcaag tagctggatg
    24841 attttgttta agtcaaccgt ttggatgacc atcgtttgat ctggtctacc tggtgcaaaa
    24901 acaagttttt catttgataa gagctcttca tcaataataa tcggaatctg tttttcataa
    24961 ccaaacggag aaacagcgcc ctgcttttgt cccgtttgtt cttccatgcg ttctgatgaa
    25021 acaatagaaa gccttttacc gacgatcttc cctaatgctt tgaaatccgt ttgtttcgta
    25081 gtaaaagtca gatatacgta gttattatct tttttatctt ttaagaataa gcttttggtc
    25141 tctgtacctg taaatccttg ttctttcttt attaccaaag catcttcatt tgtataaatg
    25201 gctcgatgat gatacaactc aaaatatata ttttcgtgta agaatttctt tgccaagtca
    25261 atcattttta tgtctctttt ctattttatt atttgagaca tcttatcata taaatatagc
    25321 ggttacaaca ttacttgtgt tttgttcgtt ttttatacat aactattgtt tttctggtaa
    25381 aataaatcaa accacttaat ggaggcgttt tagaatggaa aaatttcagc cgatcatcaa
    25441 tcaaatcaaa gaaaaagaac atcaacaaaa gatcaaagac ctttttgcta agataacaga
    25501 aacgtatccc cagctagaca caactgtaaa atggaaccaa cctatgttta ctgaccatgg
    25561 tacctttatt attgcattta gttttgctaa aaatcatttt tctatcgcac cggaaaaagc
    25621 agcgattcgt gcccttgaaa aaaatatcca agaagcaggc tacgtatata cggataacgt
    25681 catcaaagtt ccttggaaaa gtgtaatcaa ctgggaattg attgaacaat tgatttcatt
    25741 taatattgaa gataaaaaag gtcatgataa attttggcga taaagcttta tacgaataaa
    25801 ccttttactg gcgaggaaac tattcatcac cttatacagg ttttgttcaa tcctatcctt
    25861 tttatcatcc ctggttaaac acttaattga caaagtatct gctattttta taaggagtct
    25921 ttcttgtgct gaaaaaaatc actgattgaa tggctttgaa ttttctacat agtaaaatta
    25981 ttttttcagc aattttcctg caaaactttt gaataaggaa aaaatacgag aaaatatcag
    26041 tgatctttca taatactgcc cattattttc aggtatattt aaatataata tcactttagg
    26101 agagaacgaa tttctttaga gaaataaaag ttagaatttt ctgacttgat ttttacaatg
    26161 accttaatta aaatcgttct ttctacattt cgtgattaag caaatatcaa ctaagtcagt
    26221 agaaccattc acataggagg gtgaacattg aaagattttt actcagtaaa tgaattagcc
    26281 gaacaattag gtgttactac acgttctatc agaaattatc ttcatgaagg aaaactaaaa
    26341 ggaacaaagg ttggcgggca atggaaattt tctgaacgta atctttttga gttcctatac
    26401 ggagatcaag cagaggaagc agcaaaagaa atgcagcgat tcatgctaaa tgcaccaatc
    26461 acgatgcgtt tcaaccttca atatcgtgat tttacagcaa tcaatcaatt ccgagaacaa
    26521 ttagtccagt accataacga tgtttacgca aataaaaaag accgtctttt acaatacgat
    26581 ctttataaag acaatcatgc ggagatacta atcggtggta acttcaatta tgtcgtaaac
    26641 tttagtcaat ggatcaatga aaaattattg atccagacag atatttccct tgtatcttaa
    26701 acaattaaaa agcgctagaa attccagaga tgttggaaat tctagcgcta tttttttata
    26761 agtacttact gtttggttca aacaaatatt tttttaataa atgaaatttt accatcttta
    26821 tcctcaaata aagaccgtga ccatgcttcc tactacgatc agcaataatc cgataatcgt
    26881 taattttttt tcttttggtg tcttattttc atgcaggatg tacataccgc ctaatgtaga
    26941 gataactacg tttagctgtg taatgatata tgcagtagca accccatttt catttgctga
    27001 aaaaatataa gccaatgctg acacactaaa gatggctcca acgatcgttg ctttccaact
    27061 cttgtcttca gaaaatgcgg ttggtttacg gaaaatcaca taaatcactg cccctaaaaa
    27121 gacaccgatc atttgcggaa agaaaatgga aagtccgctt gcatcaacta gtttaggcaa
    27181 tgcactgtat acccaataac caatacttgt tgatgctaat agactaattc cttttgtgat
    27241 accgcctttt tttccaggat catcactgat tgccgttaaa gcagaaccaa atacaagaag
    27301 aatgattgct gctgctccaa tgattttccc catacttcca gaccattctc caaaagcgaa
    27361 gacaccaatc aacgaggttc cgactaactg taatgcagtc gaaattggca tcgttttaga
    27421 aacaccaatc aagttgaaag atgtatactg tcctgtttgt ccaataaccc aaaatgcacc
    27481 agaaagtaaa ctaatcaaaa atgtagtcac tgaaatacca gctggagaag cgaataattg
    27541 aacgagtaag ccgacaagca ccgctccaat accagttcct aatatctcat ttgttggtct
    27601 gccaccaatc tttttcaaaa tcagtggctg gatcccccag ccaactgccg ggatcaatgc
    27661 aatcaataaa cccattttta ttcaccctta tccttttcca ccttggaaag aaggatataa
    27721 agtcatccca ccatctgcaa atagtgtaat acctgtaaca taactggatt cgtctgaagc
    27781 caaccatgca gctgtcgccg ctatttcttt tggttcacct acgcgtccta gtgggatcat
    27841 actttccaag agttcttttt gttcaggatc atcaaatttt gccgcattaa taggtgtacg
    27901 gatagcacca gggcctatac tattgattct gattcccttc ggtgcatact ccattgctac
    27961 cgtttcggta aataatttta ccccgccttt tgaagcagcg taatgagcaa acgttggcca
    28021 tgggatctgt tcatgaacag aagacatatt gatgatattt ccttttttat tattctctgt
    28081 aaagtaattt aaagccgctt tcgttccgat aaaaacgcca gtcaaattga cttttaacac
    28141 tctttcccaa tcttcaaggc taagttcatg ggttggatgc tgattttcca tcccagcatt
    28201 attaacccaa acgtccagtg tcccaaaatt ttctaaagcc gtatcaagta aacgctgtat
    28261 tccttcttca gaactgacat ctgcttgtac agagactgct tttccgcctg cctcttcgat
    28321 tgctttgact gctttttctg ctccttcttt atccgagtta tagttgacaa ctacattcat
    28381 tttttctttc ccgaatcgtt gagcaatcgc tgttccaatc ccctttgagc caccagttac
    28441 tactgcgact ttgttttcta attccttgta catagtaccc actccttttt catacctaaa
    28501 cctgttatta cggataactt ttatttccgc cttaaagtga gtgtacacca agatatatag
    28561 tctaaagcaa atgatacgct ctagcaatcg ttttcatgaa ttgctagtgt ccttttattt
    28621 ctataaagaa agaggttgtg acagaagtgg tcagcttcaa gaaatttcgg cttatttccg
    28681 aagaagctac ttctgaaaaa ccatttattt ggaaaaaaag gctgtgacaa gacttttgtc
    28741 acagcccctt tcctgggctt atttatcacc cattttattg taattgctag ttatacataa
    28801 acaagagatt ttatgcataa tgttttattg ctgagaaaaa tattcagctg ttgcttgttc
    28861 atcctccttg agttgttcaa tcaattcctc tacactagaa aatttcactt gatctcttaa
    28921 aaattgattc cagcgtaccg cgacatgttc accatagata tcttgattaa agtccaaaat
    28981 atacaactct acagtcagct cccgtccttc accgaatgta tcattatgtc caattgaacc
    29041 catggctgga taccatgttt ccccaatttt tatttccgac acataaacac cttctttagg
    29101 caaatgaacc gtgctcttta ctttgacatt tgctgtaggg aatcctagta gtctgcctcg
    29161 tgcatcgcca tggacaacta ccccttctac ctcatatgtg caacctaata gttttgctgc
    29221 ttcttttact tgtccgctgt ctagtaatcc acgaatacgg gtagagctga ttttttcacc
    29281 ttgttcttct tctttagaaa ctgtgacgat tttgaatctt cctttggcat attcaggaaa
    29341 atgagcgaca tctgcaatgt cttttggtcc atacgtataa tcaaaaccgg aaacagcata
    29401 ttttgcatgt aaccctacga tgtattgatc aacaaattcc tgcggtgcta aatgagcaaa
    29461 agaagaagta aaatcaactt catataaata atctataccc aaagcggcca ttttttcttc
    29521 tttttgctta aggctagtca aatatttcac gctgctagga tcgatctttt taaagacaat
    29581 agatggatgc tgattgaaag tcatcaatgc taatttcaat ccttcttttt gtgcaatttc
    29641 catacctgtc tttatgactt tctggtgtcc gatatgcacg ccgtcgaaaa atcccagcac
    29701 taaaacgaca tcgtcttctg gaatttgttc tgattggtaa ggatgtctga tcttgatgat
    29761 attcattcgt tattcctcat ttctcaatac tttactaggt tttaatttgt ttttttcttt
    29821 cgggtttggc tgataaatac tgacaacttt tcctttataa aacaaagcaa tttcttctga
    29881 cggcatttct gggagcgcaa atacagtata atcaagtctc ataccgtttt ttactttctg
    29941 ccatacttca tcagtaatat ctactcgttt gaaccgttcc actccttttt ctattggcag
    30001 caaacaatca tcgattgtcc catttttcac agcttgagct acttcttcta atgtcagagc
    30061 atccttctgg gacataccgg cacttgctgt tcgggtcaga tcagacatgt gcgcagggta
    30121 accaagtttc ttgcctgtat ctacagctaa tgtgcggaca taggttcctt tcccgcattc
    30181 tacttcgaag cgccaagaaa tggttcctgt cacttcgtct cttgtcaatt cacttgtccg
    30241 gataaaacgg taaatctgag cctttctaac aggacgctcc actgtttcat tattgcgggc
    30301 atattcatat aaccgcttac cgttgacttt taccgctgag tacattggtg gtatctgggt
    30361 gatctcacca ataaatgaag ccatcacttc atctattttt ttttcagata aagacatatc
    30421 tactggtttt gtttctaccc gttctccgga tcgatcttcc gtcgttgtac tgaaacctag
    30481 agttatctcg cctacataga ttttcccaga atctgtcaaa tattcgataa cttttgtggc
    30541 ttttccaata caaatgggca acacaccgtc tacgtctggg tcaagcgtac ccccatgtcc
    30601 gatttttttc gtatgtaaga tttttcttaa tttgaatacg caatcatgac ttgtcattcc
    30661 gcgttctttc cataaaggta ataacccgtc catgtctttt ctccatttca tttttgattt
    30721 aactgaatta ttataacaca ttgcatcaat tactgacgaa tttgacatca taatgaacgg
    30781 tttttatttt tcggcaataa aaacgtaaca aaattcatgc tcacgtaaat agaaatcatg
    30841 ttatgctttt ttaaaggagg gaatagaatg gaagattttt tctttgcacc atttgattgg
    30901 atttatgggt ggcttacagg taaatagttt ggttttcact tcccaaaaac gaaaaaaaag
    30961 actggccatt cttccagtct tttttccgtt ttttttattt attttttcag tttcttaatc
    31021 aatgtaaaaa attctatttt gcgggatcga ttttatcatt ttattggtca agatttttcc
    31081 atttccaatc caatacttct ggcagatcaa taccattttc acgaatatag ttttgatgtt
    31141 cttgtaattt ctgatccatt tttttcgcaa aatcttctgc ttgacttcct ttgattttta
    31201 aagctgcgtc ttttgccaaa tgataacgat caagttcatt tactacacgc atgtcaaatg
    31261 gtgtcgtgat atctccattt tctttgtagc catgaatcat taaatgatgg ttatgacgat
    31321 caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa agcaaataag atcggtttgt
    31381 cttttgtaaa atattgatca aattcttcat cgctcaaccc acgcggatct tgacttggat
    31441 gacgtaattt caataaatct acgacattga tgaaccgaat ttttagtttt gggaattctt
    31501 ccagcagcaa agttacagct gctaatgctt ctaagttaga ttccgttcct gcagcagcaa
    31561 tgaccaattc aggttcctcc tcttttgctg tcgaagccca atcaatgatt ttcaagcctt
    31621 cattcactaa gacggctgct tcttctgctg agtaaaattg agcacgcgga tgtttggaag
    31681 agatgatcaa attgattttt tctgacgagc ggaatgcttt atccatgact gcgagcaatg
    31741 tatttgcatc cgcaggcaga tattcacgga tgaactccgc cttcttttct gccaaatgtg
    31801 tcaatattcc tggatcttgg tggctatatc cattgtgatc ttgctggaag actgttgagc
    31861 ttgcaatgag atttagcgaa ggataatcat ttcgccaaga aaggtcatgt gatttccgca
    31921 tccatttgaa gtgctgagtc aacatagagt caaccactcg caagaatgat tcgtaacttg
    31981 caaaaaacca tgccgtccag tcagaacgta cccttctaag aacccttcat cttggtgttc
    32041 cgataattga ctgtcgatga cgcgaccaga tggcgaaagc cattcatcat tttctggttc
    32101 gattttttcc atccattggc gatttgtcgt tttgaaaact tgattcaatc ggtttgattt
    32161 tgtttcatct ggtccaaata tacggaaatt ttctggattt ttctcaatga tctcgcgaac
    32221 gaatttacca agttcggtca tatcttctgc tttaacggag cctggttttt caaattgcaa
    32281 agtatatttt ttccaatctg gcataataag cggtcttgga tcgattccac cattggtgat
    32341 aggatttttt gccattcttt ggtcaccagt aggcgcgatt tctctgattt cttctaaaat
    32401 acgtccttgt gcatcaaata atttttctgg ttcataggat ttcagccaac ggagtaaagc
    32461 atctgcatga tccatatgtt cttgatctac aggaatcggt acttggtgtg ctcggaaggt
    32521 tccttcaatt ggttccccgt cccattcttt tggaccggtc catccttttg gtgtgcgtac
    32581 gatcaatact ggccattttg gcatcacgac atctgttgct gtcttcaggc gagcttcttt
    32641 ttggatggat ttaattttct cgattgcttg atccattttt tcagccataa caggatttaa
    32701 tttttctgaa tcatttcctt caataaagaa aggttcccag ccaagtccgt taaagtaatt
    32761 tgctaattct ccatctgatt tacgtgccaa gatcgttggg ttggctattt ttgcaccatt
    32821 taaatgaagg atcggtaaaa cagctccatc tatcactgga ttgataaaga catttgagaa
    32881 ccagcttcca gctaaaggac cggtttctgc ttcaccatca ccaatcacga cagcagagat
    32941 tacttcagga ttgtctaata cagctccgac tgcatgtgaa agagagtatc caagctctcc
    33001 gccttcatgg atagaacctg gtgtttctgg tgctgcatga gaagcaatgc ctccaggaaa
    33061 tgaaaatcgt ttgaataatt tttgcattcc tgtctcatct tccgttactt ctggatagat
    33121 ctctgtgtaa cttccatcta aataagcatt cgataccatg acttgtccgc cgtgtcctgg
    33181 cccttcaata taaaacatgt tcaaatcata tttattgatg actcggttca aatggacata
    33241 aataaaattt tggccaggaa tcgttcccca atgaccgatt ggatgttttt tgacatcttc
    33301 gggtttcagt gtacgtctca gtaaggggtt atctttaaga taaagttgcc caacagaaag
    33361 ataattagca gcacgccacc aagccgtcat tttatcaaag tattctttac tagaataatc
    33421 catacataac actccttttc attattactc ttattctagc gcagatactc ccaattgaaa
    33481 cctaataagc acttctgttc atttgtttgg attttatatc tagccaaatg aacaggtata
    33541 aaaaaccacg aaatttttcc tttcgctgac gttttttaca ttgatccaaa aaacttgtcg
    33601 ttatcacaat ggctgttccg cagttcattt cgctcctcat catgcataat ttgctgaatg
    33661 acgctgggcc agttaacaca ttgctggaaa actggggctg gatcgagcaa ttcctttcct
    33721 gattgatccg ttttatgtaa aagtctctgt tatctttgtc aatatgtgaa ttggtatccc
    33781 agctactatg ctaatttcta cagggattat ccaaaatttg gatcgtgacc aaatcgaagc
    33841 ggcgtaatcg gaaatatcaa taacttcaac gtgatttttc ttttaactgt caacggaccg
    33901 acaaactcaa acttttatgg tgcaggttcc actgatcttc tagttacttg gctatacctg
    33961 actgtcacta aaatagatta caatctagct tccgttatcg gtatcttgat ctttatctat
    34021 atgcaaaaat ttatatacta ctagaaaaaa gaaagtcgaa ttttttactt ttctttttct
    34081 cttttttggt tagctgacac gccgcaagac taaagtcatg gaactcttag gtaatacgtc
    34141 ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca ggatttgcgg gagtttttga
    34201 ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg ttgccataga caactgattc
    34261 atcaagttca aagaacaatt caggtgtctt ataaatactt agattgtgcc ctaactctcg
    34321 acgaatcaat cctgatgctt tattcaatcc atcttgtgct gttttcttat ctgatgctaa
    34381 gtcagataaa atgctgtaat agatcgttgc ttgctgtaag tcccccgtaa ctttcacatc
    34441 tgtgatcgtt acgttctcca cacgagggtc acggaccttt ttgcgcaaaa tatcattgac
    34501 ttcttttaag atttcttgac cgactcggcg gtcacggtag ttagccataa tgattggcct
    34561 ccttttatca gtttttagtc atttttgact tcttccatga taaatccttc aattacgtca
    34621 tctactttga tgtcattgaa tttttcaatc attgcaccac attcataacc cattttgact
    34681 tctttcacgt catctttgaa tcgtttcaga cttgcaagtt ggccttcata gattacgatt
    34741 ccgtcacgga ttacacggac accgctatct ctacgaatat atccttctgt tacataggca
    34801 cctgcaatcg taccaacttt agaaactttg aaggtttcac gaacgatcat ttgaccggtg
    34861 attttttctt caaattcagg gtctagcatc cctttcatcg cagtttcgat ttcttcgatg
    34921 gctttataga tgatacggtg aagacgaata tcgacttctt cagtatcagc ttgcactttt
    34981 gcctgcggtg taggacgaac gttgaaaccg atgataatag cgttgctggc tgctgctaag
    35041 gttacatcac tttcattgat tgctccgaca gctgaatgga cgatttttac acggacacct
    35101 tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg ctgaaccttg tacatctgct
    35161 ttgatgatta cgttaacttc tttcaattcg ccttctttca ggctttcaaa taagttatcc
    35221 aaagtcacac ggcttgtggc agcacggttt tctaataatg cacgtttcgc gcgttcttca
    35281 cccgctgcac gtgctgtctt ttcgtcttcg aagactacga aacggtcacc tgcttgaggt
    35341 acatcattca agccagtgat ttctactggc gttgcaggtc ctgcttcttt atcgcgacgg
    35401 ccaagatcgt ttgtcattac acggacacgt ccgtaagtat taccgacaac gattgggtca
    35461 ccaacatgaa gtgttccttc ttggacaagt aaggttgaca caggaccttt acctttatca
    35521 agacgtgctt cgatcactgt tccgattgcg cgttgtgttg gatctgcttt taaatcttct
    35581 acttctgcga ctaacaggat catttccagc aattcttcga tgttttggcc gaatttcgct
    35641 gagatctcaa caaagatcgt ttctccaccc catgcttcag gaattaactc atactcactt
    35701 aattcttgca taacatgttg agggtttgca cctggtttat caattttatt tacagcgacg
    35761 atgattggaa cgcctgcagc tttggcatgg ttgattgctt caactgtttg tggcatgacg
    35821 ccatcatctg cagcaacaac taagatcgtg atatctgtga tacttgctcc acgtgcacgc
    35881 atacttgtaa aggccgcatg tcctggtgta tccaagaaag tgattggttt accatcaata
    35941 tcgatctggt aggcaccgat atgctgtgtg attccgccgg cttcgccgct tgttacacgt
    36001 gaatggcgta atgtatccaa taaagttgtt ttcccatggt ctacgtgtcc cataatcgtt
    36061 acgactggcg gacgtgagac taatttatct ggatcgactt cttctgcctc gaagaattta
    36121 tcgatatcag caatgtcaac ttggactttt tcttgtggtt ccatgccata gtccgtcgct
    36181 aacagttcaa tcgtatcttt atccagtgct tggttttgat tgaccatgac accaagcata
    36241 aataattttt taatgatttc tgctggctca cgatgaattt ttttcgcaat atctgctacg
    36301 ttcatacctt cagtatactc taatacatct ggcaattcac ggaatttgcg tggtggtaca
    36361 gctggtttag ttgattgttg ctgtttgcct tttttacctt ttttattata acgattacgg
    36421 ttattattat taaatttgcc gcgattgtta ttgtttggtt tgttgttatt gttagtacgg
    36481 ttttgattgc cgccttggct cgttccttga cgattttggc tgctggtatt tcccgtatta
    36541 ttttggtttg ttgttggttt ttttccttga ttcacttgac cgctcccttg accgcgatct
    36601 tgataactgc ggttattttt atttttttgt tgattgttcg ttgtttcttt tttacgttga
    36661 tcgttcggct gttgattggc tggttttctg cttgcttgtt gtgcagcttg ttgtttcgtg
    36721 cttgggtttt tgaacgcttg ttgcaatttc ttctcatcat tgctagtaat cgcacccata
    36781 tggtttttca catcgatgcc aagttgttgt gctttttcca cgacgtcttt actagatttt
    36841 ttgagttctt ttgctaattc atagattctt ttgttgccca tgcaatcacc ttcctaacct
    36901 tcaattagct ccttggtctt cttcgcaaat ccggcatcca ttattccgaa taccttgcga
    36961 ggttttccga tcatctgcgt gatttctgct tcagaaaata gctcaaagca aggaacttca
    37021 taatatgaac tcttatcttt tatctttttt cttgtattct cgcttgcatc ggttgccaca
    37081 aacaccaatt ttgctttttg tttgcggatt tcagcaatgg tcagttcttc acccgtgatt
    37141 agttttcccg cgcgcatcgc tagaccgatg agattcatgg ctttttgccg attaactgta
    37201 ttcattttcc aaataactct ttacgtgctt tttggtgagt gacatagtca agaagttctt
    37261 gataaaactc atctgttaat gttgtttcga gtactcgatc gagaattttt ttatcccaag
    37321 cttgttgtac ttcttccggc tcaagtgaga catacgctcc tcgtccaggc atttttccag
    37381 ttggatcgat cgatacttcc ccttcttttg aacgggtgat tcggaccatc tcttttttag
    37441 gtttcatttc acctgaaacc acggatttgc gtaaaggaat tttcctttgt ttcatctctt
    37501 tgcctcctta ctaaagttaa gcttctggtt tttcttcgac cgtttcattg ttgaatgcaa
    37561 ttgtttcata ttcatcgtct gttaaatctg actgaacgat tgcttcatca tgcagttctt
    37621 ctgtttcttt ttgttgttct tgattttcat aaaattccgt catgtcagat tctgatttga
    37681 tatcgatttt atggttagtc aatttagcag ctaaacgtgc gttttgtccg cgtttaccga
    37741 ttgctagcga taattggtag tctggaacga caactgtgca ggcctttgga tttgtctcat
    37801 cgaagatcac atccatgact tgtgcaggat tcaatgcatt tgcaatgaat acagctggat
    37861 cttcattcca ttcgacgata tccatgtttt cgccttttaa ttcattgacg attgcttgaa
    37921 ctcgttgtcc ctttggcccg acacatgtac cgactgcatc gatatttgga tcatttgagc
    37981 gaactgccac ttttgaacga tcgccagctt cacgggcgat gctgacaatt tcaacgattc
    38041 catcatagac ttctggtact tcttgctcaa ataagcggcg caataggtct ggatggctgc
    38101 ggctaacaaa tacttgcgga cctttagacg tattttctac tcgggaaaca taaactttga
    38161 ttcgatcatg tggttgataa aattcatttg gcatttgatc ttgtttagaa agaactgcct
    38221 caattttacc aagattgaca taaatataac gtttatcctg gcgttcgacg attccttgca
    38281 tgatgtcttt ttcataagca ctgaattcgt tataaataat cgttctttct gcttcacgta
    38341 cacgctgtaa aataacttgt ttagccgttt gcgcagcgat ccgcccaaaa tcttttggtg
    38401 ttacttcaaa acggatcgta tcaccgatct catatgctgg attgattaaa agtgcatctt
    38461 tcaatgagac ttccaactgt gaatccatca cttcttccgt tacttctttt accgcataga
    38521 catggatctt tccttttttc tgatcaaatt ccacttcgac gttttgtgct tggccatagt
    38581 gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc gatcacgatt tcttttgaga
    38641 tccctttttc agcttctaaa gcgtctaacg catttaacat ttctttactc attttttctg
    38701 attcctctcc gtgattaaaa ttggatagct aagcgagctt ttgcaatatt ttttcggtca
    38761 aaagtcaatt ctttttctcg tgtttttatg cgtattttca atgtaagctg ttcagcatca
    38821 aatgattgaa gaaatccttc atattgtttt tcgccatcta ctggttggta taaggagaca
    38881 tggacataat caccaagtgc cttttcatag tcgctttctt ttttcaatgg ccgctctgct
    38941 cccggggagg atacctccag gaaataagct tgaggaatcg gatctggatc tgctgcatca
    39001 agtttttcac tcagtttctc actgacaaag gcgcattctt cgatatcgat accgccttct
    39061 ttgtcaataa atactcgtaa gaaccagctt tttccttctt ttacaaattc gacttcgaca
    39121 agttcaaaat tttgttcatc taaaatcggt gtcaccattt ctgtgactgt ttcaacgacg
    39181 ctactcaaaa gtttcgcctc cttaaaaaat taagtgcgta attccattaa aaagagtgag
    39241 cgaaaaatcc gctcactcac agttagtatc attacctata agaactatac catactctgc
    39301 ttaaaattac aagcagacag gaaaacagat gcttatagca tatcgaataa cgataattga
    39361 ttttcatctg gtagatcttt caatacgcca ttgtcgttca tatattcgat caatgttttt
    39421 gaaactttac cgcgggtggc aagatcttct ttggataaaa atggaccgtc ttttcttgct
    39481 tcaacgattt gttttgccac gttggttccc aaacttggga cagctctaaa tggtgcgatc
    39541 aacgtatcac cttcaataac aaagttaacg gcatccgatt tatacaaatc aatcattcca
    39601 aatttgaatc ctcgttccag catttcattg gctaactcca gcactgtcag ttgattcttt
    39661 tcttttacgg aagcatctaa tcctttgtcc gtgatttctt tcattgcttg tttcacagca
    39721 tcttttcctt tgcacatgga gactagatca aagtcgtctg cacgtacaga aaagtatgca
    39781 cagtaataca aaatagggaa ataaaccttg aaataagcga cacgaagagc catcaatacg
    39841 taagctgcag catgggcttt cggaaacata tatttgattt ttgaacaaga atcgatatac
    39901 cagtcgggca cattattttc tttcatcgcc gatagatagg tttctcgcaa ttcatcaggg
    39961 atcttgttcc attgtccttt acgaacagtt tccatgattt taaatgccat cccgctatct
    40021 aatccagcat ggatcaaata aaccataata tcatcacgac agccgattac ttccgccagt
    40081 gtcgcatctc ctcgtcgaat caattcttcc gcatttccta accatacgtc tgtaccatga
    40141 gatagtccgg aaatttgcag tagttctgca aaagttgtag gatgcgtttc ttctagcatc
    40201 cctcgaacaa aacgagtgcc gaattctgga atacctaatg tccctgtttt ggaataaatt
    40261 tgttcctgac ttactccaag tacttcagga ccggcaaata tccgcatcac ttccgggtca
    40321 tccgtaggga tcgtttgtgg atcgatccct gataaatctt gaagcatccg aataacagtt
    40381 gggtcatcat gccctagaat atctaatttc aatacattat cgtgaatcga gtgaaaatca
    40441 aaatgagtcg ttttccattc cgaattttga tcgtctgctg gatattggat cggcgtaaaa
    40501 tcatagacat ccatataatc gggaataacg atgatccccc ctggatgctg gccggttgtc
    40561 cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt ctgcacttcg aaactgcaga
    40621 ttattgtccc tttcgtatcc ttttacatag ccatatgctg ttttgtcggc gactgtacca
    40681 atcgttccgg cacggtaaac atagtcttcg ccaaacaata ctttcgtata gttatgggct
    40741 tctgcttgat aatcacctga gaagttcaaa tcaatatcgg gcactttgtc tccatggaat
    40801 cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt ttaagcgtgc accacatttt
    40861 ggacattgtt tttctggcat gtcaaatcca gaaccatacg taccatcttc aaaaaactcg
    40921 gaatattggc actctggaca ataataatga ggggctaacg gattaacttc agtgattcct
    40981 gtcatcgtcg caacaaagct agagccaaca gagccacgtg aacctactag atagccatct
    41041 tcattacttt tatgcactag tttttgggcg atcagataaa taaccgagaa gccattacca
    41101 ttaatagaat tcaactcttt ttttaatcgt ttttgaatga tctcaggtaa cggatctccg
    41161 tacatttgtt ttgcttttgt gtaactcaat tctgatattt catcttctga acctggaatc
    41221 ttcggtgtat aaagttcatc tttgactgga atcacttctt cgcagatatc tgcgatttta
    41281 ttcgtatttt ctactacgat ctctttcgct gtttcttccc ctaaaaagtg aaatgcagtt
    41341 agcatctcat cggttgttcg gaaatggaca tccggcaaac tatgacgatt caacggattc
    41401 gctccgccca tagaattgat caaaattttt cggtaaatgg catcttcttc gttcagataa
    41461 tgtacattac ctgttgcaac aacgatttta tccaaacttt ttccgatttc taccaaattt
    41521 tggataattt cttccaaatc atgttcattt tttaccaatt cttgctcgat caaaggcgca
    41581 tagaccgctt ttggcataac ttcaatataa tcgtagaatt ttgctcggtt tcttgcctct
    41641 tctacccctt tttgcatcat ggcttcgaat atttctccct tatcacaagc tgagccgatc
    41701 aatagatttt cacgcatctt tttcaattgg gagcgtggta ttcgtgggac gcgttcgaag
    41761 tattcgacat tcgacattga aatcagtttg aacaaatctt tcagtcctgc ctgatttttt
    41821 gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag aatctccttc tccgatatgc
    41881 tcatttagct gatcatgata aagcatgtta tgattgtcca ttgcctcttt cacaaagatc
    41941 catgctaagt gaccagttgc ttcagcatca tagattgctc ggtgatgctg ttccaagctg
    42001 acaccaaatt ttttagataa cacacctaat ccgaaacgtt tgaattgagg atacagatat
    42061 ctagctaatt ccaacgtatc aatgactgga ttggccgctt caggaatacc ataccttgcg
    42121 tagctagtat tcaagaaccc catatcaaaa gccgcgttgt gggctaccaa aattgtatct
    42181 tttgaaaatt caaggaacat ccgcaatact tcttcttccg actttgatcc tcgaaccatt
    42241 ccatccgtga taccagtcag atctaccgtt gttcgtgaca aaggatgtcc tggatcgata
    42301 aattcatcaa agctttcgat cacatttcct ttatacattt tgactgcggc tagctcgatg
    42361 atcgtgtcat atactgcaga caatcctgtc gtttccacgt caaatacgac atatgtggct
    42421 tcactcaatg cttcatgtgc atcattataa gcaattggga caccatcatc tacgacattc
    42481 gcttccacgc cgtataagat tttcacacct gctttttttc cagcactatg tgcttccgga
    42541 aaggcttgcg caccaccgtg gtctgtaatg gcgatagctc ggtgtcccca tttccctgct
    42601 tgtgccacaa gatcagagat gctgtttgtt gcatccatcg tactcatatt gctatgaaca
    42661 tgaagctcta ctcgtttttc cccttcagga gcataatctt tgcgaggtgt atgtttgact
    42721 tctatgatat cttgcgcatt catgaccaaa tcacggacaa acgtatcttc ttgaatactt
    42781 cctcgtacct ttaaccagct accgacactg atcgcatcaa atacttgttc atctttttcg
    42841 ccgttcgaga attttttgac gataaatgat gaagtataat ctgtgatttt caaagtcaag
    42901 atcttgcgtt ttgaacgtag ttctctgact tctttgtcga atacgtaccc ctcaagtgtg
    42961 actcttcgtt cttcttcaac gatactgatc attggtgtcg ttggttcatc ggctggaata
    43021 tttcgtccta gctggatgtg gatgggtcct tcaagagctg gcgattgctc ttttctttcc
    43081 tttttctttt gttcgtggac aactagactt tcagctgctt gcttcataaa ggcttctgcc
    43141 tgttcttgtt tgcgttcttc aaaaagtttc aatactcgtt ccgcttgctg ctcatccact
    43201 tctggttcga tacgaaattt tgggaatccg taactgacga acaactcttc tactaatggc
    43261 aagtattgtt gcttcaaata agaaatagct cctgtactgt cgattggcaa gatgacttta
    43321 cgatctttta tcacaggtac ctgcgttttc aatacttgct gtgctagtgg tgtatcgcat
    43381 tgctgatttt ccaaagcttg ggcccagtaa tcttgcaaga gctgttcatc gaatgtctgg
    43441 tcatctgcta agatattcag tttgacttgt gcaatatctt taaaagcgat agtcagatgc
    43501 tgagtcaatg accgatatag cataataggc aaaattttgg aaaaatgcag cgtaaattcc
    43561 cataaacgcg atttacgatg gaccactaca ctatccattt caccagcagt aagcagtgga
    43621 tgattttttt ctgcttcctc tagttggatt tgttccaaca gtttttcaaa taaatctcgt
    43681 ttttctgaca aagatatacg ccctttctga tgagaaacaa tgcaattgat cgcatcttat
    43741 cctcttcttt ttcagtacct tttagtatac tcgttttttc tgagttttac attaaaaaat
    43801 tggaactgcg attcaaatcg cagttccaat tttaaaagcg aatcatcaga ttgtcaaaaa
    43861 cagctgattc agctattttt attttctgtg ttttaacgct tattattcgc cttggttcaa
    43921 gaggattggt aaagtattca ccaattcttc tttacgtact tcaaccattt cgccagtttt
    43981 cttgattttc acttctacga ttccatccac tgctttttta ccgacagtga ttctgattgg
    44041 acagccaatc aaatcagaat ccgcgaattt cactcctgcg cgttcatttc tgtcatcaac
    44101 cagtacttca tagccagctt ttgtcatcgc ttcttctagt tcattcgtca ggtttgtttg
    44161 gtattcgtct tttagattca tttggacgat atgcaaatca aatggcgcaa ttccttttgg
    44221 ccagttgatt ccttgttcat ctgaatgttg ttctacgatt gctgataata aacgacttac
    44281 accgatacca taacagccca taatcacaaa tttttctcgt ccattttcgt ctagtacagt
    44341 tgctcccata gattcactgt aacgagtgcc gattttgaag atatgtccga tttcgattcc
    44401 tcgagtaaag gcaagaacac cattgttgtc tggtgaagga tctccttctt gtacaaaacg
    44461 aagatcttca taagaaagta cagtgaaatc gcgttctgga ttcacatttt tatagtggaa
    44521 tcctgtttcg ttcgctccag taatggcatt cgctaaatct tggacataac ggtctgcata
    44581 tacttttacg tcatctgaaa caccgactgg tccgacagaa ccaaactcag cacctagata
    44641 tttttgtgct tcttcttctg ttgcttcttc taaaaagtca gcagataaaa agtttttcag
    44701 ttttacgtca ttgacttcat ggtctccgcg taccaaaaca aggacaggtt gttcatccgc
    44761 aatgaacaat acagacttga tgattttttg aggttcaaca gaaaagaaat ctgctacttc
    44821 gtcaattgat tttacgttag gtgtagctac tttctccaat tcaagctgtg tttcatgcga
    44881 tttttttgat acatagtagc ttgtagccat ctccaagttt gcagcataat cactttcagt
    44941 cgaataacag atcgtatcct ccccgatttc tgagatcgcc atgaattctt ttgagtcttt
    45001 cccgcccatt gcgccaccgt ctccgataat cgagcgaaat tctaagccac aacgcttgaa
    45061 tacttcggtg taggcttttt cgtaatcttt atatgtctcg tccaaacttt cttccgttgc
    45121 atggaaagaa tacgcatctt tcatgataaa ctctcttccg cgaagcaaac cgaagcgagg
    45181 tcgtttttcg tcacgatatt taggttggat ttgataaaga tttaaaggta atttcttata
    45241 tgaattgatt tcattacgca tcaaatcagt aaaagtttct tcgtgagttg gtccaaggat
    45301 catttttcga tcattgcggt ctttgaaacg atacaagttt ggtccatatg tttcgtaacg
    45361 cccggattct tcccaaagtt ctgctggcaa gatggcaggc atcagcattt ctaccgctcc
    45421 gattttttca aattcttccc gcataatgcg tttcagtttt tccaatacac gatttgctaa
    45481 tggcagatat gaataaatac ccgctgatac ttggcggata tatccagctc ttagcaagat
    45541 ttgatggctt aatacttctg catcatttgg aacttcgcgt aatgttggaa tcaacatttt
    45601 tgattgtctc attctatata ctcctttatt tcctcaaaat agttatcgtt tgttattata
    45661 ccctatttgt caactttttc tagaaaaaga atcgttgaat atcgttccat gtaactagca
    45721 ccatcaacaa cattaaaaat ccaaagccag ctaatgtcaa tattccttct ttttcttgac
    45781 ttaatggttt cccgcgaatc ccttcaaaaa tattcaagac taatttcccg ccatctaagg
    45841 caggaatcgg aagcagattg acgattccga gattcattga aagaagcgcc atcaaactaa
    45901 taacagttgt tacaccttga tttgctgctt ctgaagacaa ttggtacatc atgacaggtc
    45961 cgcctaattt atctaaactg aaacctgtaa atagtgatcc taaggctttg aaaatttcca
    46021 atgaaccgct gaaagcttga cgcgtccctc caatgatctt atccataaaa cctgtattca
    46081 ttggggcttt tataccaagt tggccaactt tttcgccatt tgactctaca gatttcggtg
    46141 tcacgtcgac agattgcacc tgaccttctc gttcgatctt aaagtctaat gttttatccg
    46201 gatttttcgt aatgactgtt gtcagatcat tccatgaatg gatttctttt ccgtccacac
    46261 tgactacctc atcattttct tttaagcctg cttctgccgc agctccatta ggcatgatct
    46321 ctcctacgcg attagtgttc gtcacttgaa caccaccttg cataaatgcc agaatgatga
    46381 ataacaggat tgccaaaata aagttattca tgggtccagc aaagttggtc agcatacgct
    46441 gccacaattt agccgattgg aactgtacgt cttttggcgc aattctgatt tctgttccgt
    46501 cgttttcaat aatcgtcgca tcgtgatcaa cagggtaggt tactacttct gtttcgtctc
    46561 cattcacata accagtgata gtcagctcat cttctaaatc ataacggctc aattccatcg
    46621 ggatagcatt tgtcaattgg attttcttac ttaaatttat cttctctacg atgccgtcag
    46681 aatttagtaa aagagaaaga ggcattcctg gtgccatttc tgtttcatca tccccattgc
    46741 ctgccattcg aacataaccg ccaataggta ataggcgaag cgtatatgtt gtcccgtctt
    46801 ttgcttgatg cccataaatt ttcggaccca taccgatagc aaattcgcga acgaggattc
    46861 ctgatcgttt cgcaaagaag aaatgaccaa actcatgaac aatcactaat ataccaaaaa
    46921 cgatgataaa tgtcagaatc gttttcatag cttttttctc ctttacttgt tttttgtgtt
    46981 ttttataaaa caacgataaa acactataat tttaccatag tataccaaag acaccaatag
    47041 tatcaatata atgaaaaata aaaacctgcg tcttttatga aattcacaaa gaaacgcagg
    47101 tatacttact aaataatgcc aaataaatgc atgataggaa atacaaacag caaactgtcg
    47161 aaacggtcta agattccacc atggcctggt aagatatttc ccgagtcttt cacatcataa
    47221 tgacgtttga ttgcagattc gaccaaatca ccaaattgtc cgactgctga aaaaataatc
    47281 gtcatcagaa gcatgacaaa cataccatga ttaaatagtt cttttgctgg gtatagcaat
    47341 aaataaagaa atgcgactac aacagcacta ccaataccac cgagcgcacc ttcaatcgtt
    47401 ttgttgggcg aaatatcagg acttaactta tgctgaccat aacgtcgacc aaccatatat
    47461 gctccgatat cagttgccca aacaataaat agaccaaata tcaaaacagc caagctttct
    47521 gttcgtgcat tgacaaaatt ctggaaacct attcccacat acaagctgac aagcacagga
    47581 aaacctgctt catcaatcgt atacgtattt ttagaaataa ctgatgttcc taaaagaatc
    47641 ataactataa gataaaataa cataaatgta cttacattct ctggaagaaa gaaaaaccat
    47701 ctttcttttg gcaaaacaag taacactgca ccaacggcgg ataaaactcc ttcaaaactt
    47761 aagagtgtca atcctttcat ccgaaacagc tcgtaaactc ctattactgc taagattgct
    47821 gctgtaagct caaccgctat gcctccgatc catataatcg gaataaacaa gactagcgcc
    47881 acggctgctg tgaccacacg ttgtttcatg actgatcccc tttctcatct tctgtttttt
    47941 ttacaccgcc gaatcgacgg tctcgatttt gataagaggc gattgcttct tcaagatgcg
    48001 caccatcaaa atcaggccaa agcgcttttg taaaatacag ctcactatat gcgatctgcc
    48061 ataacagaaa attactaatt cgctcttctc cacttgtacg gatcatcagc tcaggatctt
    48121 gtaactcttt aggcaagaaa cctgtcatta aatgcttagc aatcaattca tcatcgattt
    48181 cttctgcgtg tatttctcct tttgctactt cttcggcaat ttcttggaca gcagtcacga
    48241 tctctgctct gcttccataa ttcaaagcaa aattcaatac cattccagta ttgttttttg
    48301 tttgctcaat tgcacgtctt acagcatctt gcgtatgttc tggcaatacg ttttcataac
    48361 ccatgacatg aactttgaca ttttctttaa tcaattctgg tacgaacgta tcaaagaagt
    48421 cgacaggtag ttgcatgaga aaacttactt catcttttgg tcgcttccag ttttcagtcg
    48481 aaaaggcata aagcgtcaac actttcactc caagccttga tgcttttttt gtgactttct
    48541 ttactgtttc catgccttct ttatggccag caactcgtgg caaacgacgg ttttgcgccc
    48601 aacgaccatt gccgtccatg atgatggcaa tgtgctgagg aattggtcca tcggtattaa
    48661 attgaaattc gcttttttct tgaatatatt tgtttttttg cggaaaaaaa cgtaacattc
    48721 tttttcctcc tatacttaaa agattcccac tatcttaagt attatagcaa tatctaactg
    48781 tttttcctat gaaatcattg tttttttatt taaaaactga ataatttttt ctttttttaa
    48841 tttttaaata aaagaaatat aaataaaaac agaggaagcg gaatgatccg cccctctgtt
    48901 tttatgattt tattaaactt ccaataattc tttttcttta tcagcagcga tcttatctac
    48961 ttctttgatg ctgttatctg tcaatgtttg tacgtctttt tctaatccgc gcaaatcatc
    49021 ttcggtaata tcaccatttt tttgttgttt tttatattca tcgatcgcat cacgacggat
    49081 gttacgaatc gcgatttttg cattttctgc ttctttttta acatctttag ctaattcttt
    49141 tctgcgttct tctgtcagtt gcgggataac taagcgaatg acatttccat cattggttgg
    49201 actgattccg atatcacttg ccataatagc tttttcaaca tcttgaagaa tgctcttgtc
    49261 aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg attgatgcca tttggttcaa
    49321 tggtgttggc gcaccgtagt agttcacagt aatacggtcc aaaaggctag cgttcgcacg
    49381 gcctgcgcgg atttgcccaa gttcacgttg aagattttgt gctgcttttt tcattttttc
    49441 ttttgcttct gtcataattg catctgccat ttttatttcc cccttacagt tgttccgata
    49501 ttttcgccta agattgcacg gcgaatattt ccgtgttcat ttaaattgaa gacaagcagt
    49561 ggaatatcat tatccatgct tagtgaactt gcagttgaat ccatgacttg taatcctttg
    49621 gcaataactt ccagatgagt cagttcttca aattttactg catttgcatc tactttcgga
    49681 tcagcagaat aaacaccgtc tacattgttt ttagccatca ggataacatc tgcgccgatt
    49741 tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc ggtaccgcct
    49801 gcaaagataa cgatccgtcc tttttctaaa tgacgttctg ctttacggcg aatatatggc
    49861 tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc cacgttttcc
    49921 aacgtatcct gtaaagctaa ggcattcata actgtagcta acatacccat gtaatcagct
    49981 tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc gccagatatt tccgccgccc
    50041 acaacgattg ccatttcaac gcccagctcg tgaacttctt tgatttcttg gatgatttct
    50101 ttgattactg gtggtttgat tccaaatcct gcgtccccag ctaaagcttc tccactcaat
    50161 tttaagacta cacgctgata tttcggtgtg accatgttca ttcctccaat attttctatt
    50221 gccattttac catacttaga tgatttcgac actctaagac tggtttttct tgttttgctt
    50281 aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa ttattttttc
    50341 acttggctca taacttcttc tacaaagtta tcttcgcgtt tttcgatacc ttcgccaact
    50401 tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg caacgtattt ttcaacagtc
    50461 atatcaggat ctttaacgaa tggttggtcc accaaagcga tttctgcttt gaatttcttc
    50521 aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt caatgcttgt
    50581 tctgtaagaa cagttttttc atgttctaat tcagcttctg gaatttgtga ttcgtttaca
    50641 taacgaggat tgatagctgc tacgtgcata gctacgtctc ttgcaactgt ttcgtcagtt
    50701 gttccatcta gaacagtcaa tacggcaata cgtccgccca tatgcaagta tccaccaaat
    50761 gcagcattgt cgtctttttc tacaacttca aagcgacgga agctgatttt ttctccgata
    50821 acttgagttg cttcgatcaa gtcagattca attgtacctt tatctgtttt gattttcatt
    50881 gcagcttcca tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc ttttactaaa
    50941 tcttggaaca tttcgttttt agaaacaaag tctgtttctg agtttacttc aacgattgct
    51001 gcagtgttac ctttcacagc aactgaagcc aatccttcag ctgcaatgcg gtcatttttc
    51061 ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa ctgctttttc catgtcgcct
    51121 tctacttcaa ctaatgcttt tttcgcatcc atcattccta cgccagtcat ttcgcgtagt
    51181 tcttttacca ttttagctgt aacgtctgcc atgtttaact cctcctgtgt gtttttcttt
    51241 ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga aacagctcca
    51301 tcattcaaaa ttattctgct gaagaattgt cgccttcaac aacatctacg atttcttcga
    51361 ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac tacttgatct tcaccttggt
    51421 ttccttcgat gaaagcatca gccatttttg aagtaatcaa tttaacggca cgaattgcat
    51481 cgtcgtttga agggattact acgtcgattt catctggatc gcagtttgta tcaaccatag
    51541 ctacgattgg gatatttaat ttgtgtgctt cttgaacagc aatacgttct ttacgtgggt
    51601 caacgatgta cattacatct gggattcttg gcatatcagc gataccgcct aagaattttt
    51661 caagacgttc acgttctttg ttcaaaccag caacttcttt tttaggtaga acttcaaaag
    51721 ttccatcttc ttccattgca ttgatttgtt tcaaacggct gatacgtttt tggatagtat
    51781 cccagtttgt caatgttccg cctaaccaac gatggttaac gaagtattgt cctgcacgag
    51841 ttgcttcttc tttgattgct tcttgtgctt gtttttttgt acctacgaat aatgctacgc
    51901 cgccttcttc agcaacgctc ttcatgtaat cataagctgc atctactaat ttaactgttt
    51961 tttgcaagtc aatgatgtag attccgtttc tttctgtgaa gatatatttc ttcatttttg
    52021 gattccagcg acgagtttgg tgaccaaagt gtacgccggc ttctagtaat tgtttcatag
    52081 aaattactgc catgtttgtt tcctccgatt tggttttatt tttcctcttc ttatcttcta
    52141 ccttgccggc aaactttttc aagcacctac cgacaatcca ataagaatgt ggatttgctg
    52201 atgcataaag caccctcagc tattatacag acaaaagatc cgtatcgcaa gagcaaatat
    52261 acttttctgc tcaacattca atagtttacc tgaaagataa aaagaattca agaaaaacag
    52321 tagatttctc tttgtttctt tattatcctt tttccataaa ataattttag gtataaaaga
    52381 cgaatattat tgggcaatcc ttcttttttt tgttatgata catgtgaaat ctttcgtaaa
    52441 ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat taaacaagtc attgaagaat
    52501 tcccgatccg tacgcaagaa gaactattaa acaaacttgg gcattatggc gttaccgcca
    52561 cgcaggcaac gatttctcga gatatccgtg atctaaaaat cgtgaaggca cctgatgaaa
    52621 atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact aggactgagg
    52681 aagaggatcg gctggttcaa atgattgaag acattgtatt gaaagtcgaa cgagtccact
    52741 ttttgacgat cgtcaatacc ttgccagaca atgctcattt atttgcatct gttttagatg
    52801 agatcaaacc tccctctatt gtcagtacaa ttgctggatt cgatacgatc atggtcatct
    52861 cggaaaatga ggaggacgca aaacgagtag aagaatttct tcaccatcca actgaagtat
    52921 cactttttta atcagagcac gtattcctaa tatggaatgc gtgttttttt attttcaata
    52981 ttttccttat tgaataaacc tttacactct ttacaaaaat acatcatttt ttattcaaat
    53041 ttttcaacac cttgattgaa tcacaataat catcatttat attggatgtt aacaagagca
    53101 gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg tataaattat
    53161 taacttctgg accattgacg acttctgaaa cagtgaaaaa tgctatgctg atcgatcatt
    53221 gtacttggga tcaagaatac aaagaaatta cacaatggat aagaaaaaaa ttattggatc
    53281 ttgctcaagt atcggaagga atatacacaa cggtcttaat gcaaggtagc agctccttcg
    53341 gggtagaatc ggtattaagt tctgtgatca ggccagaaga tactgtactg atttgttcaa
    53401 acggcgcata tagacaacga atgatcaaca tgtgcgaatg tctgaaactg aactataccg
    53461 tttatgaggt atcggaacat caaatcctcc aagctcatgt gattacacag ctgattgaag
    53521 agaatccgtc tatctccgca ctagcaatgg tccatagcga gactacttcc ggtattttga
    53581 ataatttgga agagattgct gctgtcacac aagaaagaga tcttttgttt atagtggacg
    53641 cgatgtcaag ctttggtggg ataccgattt gtgtagccga actagggatt gattttttga
    53701 tttccagctc aaacaaatgt attcaaggtg ttccaggctt ttcttttgta cttgctaata
    53761 aagcggaact ggaaaaacga aaaaatgtta gccgaagttt gtcacttgat ctatatgacc
    53821 agtaccgcgt gatggaaaac gagaatggca aatggcgttt cacttcccct acccatacgg
    53881 ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc gaaactcgat
    53941 attcacgtta tctcgccaat aatcatgtgc tacgacaaaa aatgacagag atggggttcc
    54001 agcctttcat tcaagaatat caggggccat tcatcacctc ttttctttac cctaaggact
    54061 ggcgatttga ttttaatcat ttttatcagt atttaaagca aaatggattt gtgatctatc
    54121 ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa tattggcgag atctatccgg
    54181 aagatatttt ggaactgaca gcaattatca aagactataa ggagaacgtt aatgattaaa
    54241 actgtaattt ttgattgggc aggaacaact gtggactttg gctgtatggc tcctgtccac
    54301 gcttttagaa atgcttttct agaaaaagga atccaactga ctgacaaaga aatccgctaa
    54361 ccgatgggaa aattaaaatg ggatcatatc caacagcttc tttccctccc ttctgtcaaa
    54421 aaacaatgga tagctattta tggacaacta cctcaggaaa aagaggtgga ggaattgtat
    54481 caacaatttg aacattatct gtttaaagat ttggtcaagc attctacctt gaaacccgac
    54541 acattggaaa cgatcgaacg gttgagaaac gctgggatca atattggttc cacgaatgga
    54601 tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa aaaaagaaag gtattcacca
    54661 gacttcatgg caacttttga agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc
    54721 cataaaaaca tgcagtactt caaaaacaag tcggttgatg aagtaatcaa agtcggagat
    54781 acggtctcag atatccagga agggaaaaat gccggagtgt tgacagtagg tgtaattgaa
    54841 ggtagttcgc taatggggat ttcttatgat gagtaccaaa aattagagat ggggaaaaag
    54901 atcgtttaaa acagtctgtt gctcttagat tccaacaagc cggcgctgat atttgtgtaa
    54961 acaatttaaa agagttaaca gaatacattt tgtacgctga catgaatcca gaatgacttt
    55021 tatacaaaat aagaggttgt gaaaaagctg tcctgtatca agtaataaaa acagccaagc
    55081 aaaaatagct cctcatattt ttcgtttggt taggcttatt accgcagatg caagcttttg
    55141 aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg acttttgtca cagtcccctt
    55201 atttgcaaac aaacagtgtt taagagacaa cctctccact aaacggtgtt cattcagctg
    55261 actctcggta tttgctcaaa aaacgataaa atttgaaaag ctattttctc ttttgtttct
    55321 ctaatactcg gatcagaacg ctgatttcac attctctttt tcttacatct tatctggtgc
    55381 atgaagtcct agcaaacgaa ggtcttcttt caataataca gtcactgcat aaactagagc
    55441 taagcgtgct tctttttgtt catcatcagc taaaatcttc gtatgagcat agtatttgtt
    55501 gaatgcttgt gctaatttga ttgcatgttt cgcaatcact gacggttcgt atttttcccc
    55561 agcagacaag accgtttcag ggtatttttg aactaattta accacttccc agctggtatc
    55621 gtcattcaat gcatagtctg ctgtttctga agggacaaat cctgcttttt ccaacaggct
    55681 gactgcacga gcatgtgtat attgcacata aggtccagtt tctccttcaa atcgaacaac
    55741 ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac
    55801 tgccccaacc ccaacttgtt tagctacagc atctttgttt tccaaatcag ggtttttctc
    55861 gctgatttgt tctttagcag attggattgc ttcatttaat acttcttcta ataagacgat
    55921 ctttccttta cgagttgata attttttacc accttgcgtg atcaaaccaa atggaatatg
    55981 gtgcatgtcg tcagaccagt caaagcccat ttccttcaag acagctttca actgtttaaa
    56041 atgatagctt tgttcattcc ctactacata tagagattgt ttgaagtcgt aagtacgttt
    56101 acggtacaat gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt
    56161 aatcaacgct ggattcaggt cataagcaga taggtcaacg atctctgcac ctttatcctc
    56221 gttcagcaaa tgtttttctt ctaaaagttt gacgatttca tccatcttgt cattgtagaa
    56281 agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga tcatagattt tattgaattc
    56341 tttcatcgat tcatcacgga accattgcca taattgaata gcctctttat cgccttcttc
    56401 cagacgtttg aaccaagcac gagcttcttc atttaattct ggttctgttt cagctacttc
    56461 atggaattgg acgtataaac gaagcaattc attgattggt tctgctttaa cagcttcttc
    56521 tgtaccccat tttttgtaag cgacgatcag ttttccaaac tgtgttcccc agtcacctaa
    56581 atggttgatg cggattggct gataaccgat tttttccata atgaaaccga tcgaattacc
    56641 gataactgtc gaacgcaaat gtcccattga gattggtttg gcaatattag gagaagacat
    56701 gtcgattggt actgttcctt gattcccgat gttgctatcc ccataatgtt ctttttcttt
    56761 cactactgtt tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa agttcaagta
    56821 aggtccgaca acttcgattt tttcaaagtt ggcagaatcg attttttctg ctaaatctgc
    56881 agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac
    56941 atctccgtgt tcagctgatt ttggattttc tagtaattgc tcgacttgtt ccagtgttaa
    57001 atcatctttg acagcgtcat agacagcttt tgcgacaaga tctttattat tcatctttat
    57061 tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat ctctagcgac ggcatactta
    57121 gtatgccatc actagagacg agatttcccg cggtaccact ccaattgtcc taaaaaagga
    57181 ccagctccat ctgatatcgg tagacgaccg tctgtttaaa acagaattct ccaagtgcgc
    57241 ttcgttgtat cttcttcatt cggcttccac cacttccgaa ttcgctagcc aagaatgata
    57301 caagtactct cttgttcaac aaatcttagt cttattaaac tttcttgaat tatatcagaa
    57361 aaaaaaacga aaggctagtc gtttcataaa aaaacaatcc agatggccta aaggagaaga
    57421 aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac aaattaatca
    57481 gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc taaagaagtg
    57541 atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcttc tactttcgga
    57601 agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt gtttcatttc tgaaacagta
    57661 acagtttcta atttcttttg atctggtttt tggtaattga catacacatt gtctactcct
    57721 gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga agcaaaatct
    57781 ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac aggaatccca
    57841 ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat gacacgtgct
    57901 tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg tccagcatct
    57961 tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc ttccgtgtag
    58021 aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc cacaataact
    58081 tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac ttcgcccatg
    58141 gcgttttgta accagtaacc gatagatcct tctgtcattg caacacatgt atctaaaggc
    58201 attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt accgacttgc
    58261 ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt cactaaatac
    58321 tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt agacaagatg
    58381 gcattgcccc ctaaagcgac aactactttt cgtttggcca tttcattcgt cctttcttca
    58441 gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc
    58501 tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta
    58561 cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc ttggtcaaat
    58621 tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt gatgccaaag
    58681 cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc
    58741 aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt gatttggtaa
    58801 ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga tacccataca
    58861 tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt aatcatcaat
    58921 tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc ttcaggttgt
    58981 aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt gactaataaa
    59041 gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc ttctactgtt
    59101 ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg ccattcatca
    59161 gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac cattttttga
    59221 ctgaatccac ggaattcgat tccgtcaaac atacgtccta gtactttagc tgtatcttct
    59281 gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg atgtgcacct
    59341 agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa
    59401 agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt caagtctttc
    59461 aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc tttttctgct
    59521 aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct ttagattgtt
    59581 tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagactg ccgtcaggaa
    59641 acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg accaccacga
    59701 cctcgtgaca attcacttga attgatttct aacactttga tgccatagct acgaagcaat
    59761 tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat tgccaatgtg
    59821 tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt accagttggg
    59881 atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa gttatctgaa
    59941 tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat cttaccgtct
    60001 ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt aaatacggta
    60061 tccaaatgca tcattgcacg gttgttcgga attttgattg ccaacacttt ttcaaatcct
    60121 gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt agttcgttga
    60181 gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc ttcgatatga
    60241 tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct tggatgatgt
    60301 ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt catcttattg
    60361 actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt
    60421 gggtccatgt aaaatggata gtcatcgtct gctgacagat catatagact gctagaacgt
    60481 acttcgatat cttttgtacg aaccccagcc atgattttat ctaccatttc ttgcgtttcc
    60541 attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat atgagattca
    60601 tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc ttctgccgct
    60661 aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtcttcgc aaagttgtca
    60721 tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg atccataata
    60781 tctggtgtca aattttccac ttcttgtcca ggccgtttca gtaaaactgt ctttaatttc
    60841 cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct tctttttgat
    60901 tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc attcaaaatg
    60961 agacattcct cacattaata ttcgataatt aaataattaa acgtttttct atttttaagc
    61021 gtaatttatt cattttttat tctgtataca ctttcaaaaa aatatttatt cattgaaaaa
    61081 atttttttga ataaattaaa aaaataaatt atattatcgt tgttagcgtt tactatatgt
    61141 attattgttg ttttttatac acaaatcatt cgcttcaaaa caaattattc aataccattg
    61201 tatatttttt acataataac aaattatatt tgtaaagtat taaaacttgt ttctaatgtt
    61261 ggtatgttaa aactttacaa attctttaaa aaaagtgaca ataaatatag tgaacaataa
    61321 ggagaggact ttttaatgcg tgttaataaa atcgattttc acttttcaaa actcagatat
    61381 caacctgatt ttgaacattt ctcggatgaa gagttccaac agatcaagga acatactgtt
    61441 cttcgttctt ataaaaaagg acagatcctt ttcgacgatg gtgatcccag acaacggttg
    61501 tatatcttga ccaaaggact cgttcgttta gaacgttatg atgaaagtgc aacttattac
    61561 tactatgact atgtgaatac aaataacctt tttcctatgt caggaatgtt tggagaagag
    61621 acctacactt atacagctca agcgatgact gatattgaaa cattctacct tcctgtttcc
    61681 tactatgaaa aattagcacg aaaaaatccc gaacagttga tctatatgat acaaaaaatt
    61741 tctaaagtag ttgaaataca tgaattgcgt atccaaatcg gattgacctc tagcgccttt
    61801 gaccgggtaa aacaaaattt attcattttg aaagaagaat tgggcgtacc aacaaaagat
    61861 ggtcatatca ccattcctta tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg
    61921 acacgtgaaa ctgctggaca agtaatcaaa caactacgtg attccgggct gttagattat
    61981 gacaaaaagc gttttacttt ctgtaagaca gctactactc cagctatgag agaataacgc
    62041 gaacaaaaat aagcagtgac cctgataaat atcagggtca ctgcttattt tcctccctaa
    62101 aacttctgaa agatccggca gttttagatc actggacaag actcaaagat tttctatgtg
    62161 tgtttcagtt atgtaatatt cactgtttgc aaaaaatact gttagattaa ctagatgaag
    62221 cgtcttgtcc tcatccgatc tagattattc gatattcctc gacaataaac gattactcag
    62281 tgagggcaat cagaggaacg gataatgata aataagctgt aaaaaatagc aaaataacaa
    62341 taatgattaa aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca gccctatttc
    62401 gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga
    62461 ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc
    62521 ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac
    62581 atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcagc tttttcacaa
    62641 cctctttatt cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt
    62701 cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt
    62761 cgaagagggg gaatcactag ttctacctca gggagtttag ctgccaaagc atcctgcaat
    62821 ccttcacgca acccttgatt ggccgctaca cctccagcta taatcagttg cttgactgga
    62881 taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag
    62941 ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa
    63001 ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc
    63061 gctcgcggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg
    63121 cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca
    63181 tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta
    63241 tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct
    63301 gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca
    63361 aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg
    63421 acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag
    63481 cataaggtga tttgttctac gtgatggcgg ctagctactt caggtactac gccaccaaaa
    63541 cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta
    63601 acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc
    63661 atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc
    63721 gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg
    63781 ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg
    63841 taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag
    63901 caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct
    63961 ggttatcttc agctaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact
    64021 gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt
    64081 ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt
    64141 ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga
    64201 tcgttgttgc ttgaaacccc aatttccgat aaagtctttg tgcatcttga ttactcatac
    64261 gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa
    64321 taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat
    64381 ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga
    64441 tacctagata tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc
    64501 atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg
    64561 tagtcaattc tcttagtacg taacgacgat cgatttgttc gactgttttt tctgtataaa
    64621 tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg
    64681 tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctactcgttt taaatagcga
    64741 ggaagaaaag cttgtttctc tgcaactggt tcaactaaag cacctaattg agccacagtt
    64801 acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc
    64861 tttatttcag agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc
    64921 ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacagtttct
    64981 aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac
    65041 atgggcacaa taatattatc cacaccgatg caattagccg cgatggtttt cagactagat
    65101 actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata
    65161 cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga
    65221 cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg
    65281 cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc
    65341 aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg
    65401 acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca
    65461 aaactagtac aataatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac
    65521 acaaaaaagc tgcggtatca ctccattttc ccacagcttt tccatctttt atttcgttga
    65581 ttctcgaata atcaactctg tttccacaat aatctttctt gaaaccggtg ctttggaatt
    65641 acttaattga agaatcgtct ccactgctag ctctcccatc cattcggtat aaacttttac
    65701 agtagaaagc gagggcgtta catatttcgc tacactgaca tcattgaagc ccatcacaga
    65761 aatatcttct ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa
    65821 tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta aatattgctt
    65881 cactgcttga tatcctgact cgacagaaaa atctgcttcg ataattggac cttcgtcgta
    65941 gcctttttct gccaaaactt gttgaaatgc cataattctg ggatctttta gccgctgatg
    66001 gtttttcttc gtatattctg ttccagcaag catagcgata tgttgcgctt tatgggagac
    66061 aaagtgttca atgacttttt gaacacttga cttaaagtcg acaactagag agtcgattcc
    66121 ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt tgggatcctt tcatttctaa
    66181 taactgagct tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa gatcactaat
    66241 ggaatcccac gtttctttca ataaagtaac gtttgcttct tctgcctttt tttcgattcc
    66301 taggcgaata gataaataat aaagatcttc aagctcttcc gtatcgtcgt accactggat
    66361 caggcgaagc gtttgcttag tatttgctgc atttttacga tgtttcgtat aatttaagcg
    66421 ttctgctgct tcaaaaattt tttgctttgt ggctaagcca acggaaagat caggatcata
    66481 gttgagaact cttgaaacag tagcaggaga aactcctgcc aactgcgcaa tgtctttaat
    66541 tgttgccatt tgtctctcct ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac
    66601 gctgcctgtc ctttttcatc tcgcttgaat actccagcgt cttccagtac acgtgcaaaa
    66661 acctgaccta cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt
    66721 tcttttttta attgttctgc ccacggacga tgatattcag ccatctggtt ttcttctcct
    66781 aataagaaat gctctacttc tactagttct ggtttcaaac gaggtggtaa aacagcaagc
    66841 cccattactt cgatcaaccc aatgttctcc tttttgatat gttggacatc tttatgtgga
    66901 tgaaaaatcc catcaggatg ttcttctgaa acattgttgt ctcgaaggac aaggtccaac
    66961 tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct
    67021 gctgaaaaag ctctgataga tactgtttca tccgaatatg ctttccattt ttccaatact
    67081 gagttagctg cttcaataag caagtttttc tctgttgatt gcaaacggat cacagacatc
    67141 ggccatttca cgactcctgc gatgacttgc ggataatctt ctagtttgat aagtttttct
    67201 atcggtgctt ttgccatagg aaattcatga cgtcctgcct gataatgatc atgagaaaga
    67261 atcgatcctc caacaattgg taagtcagca ttcgaaccaa caaagtaatg aggcaagact
    67321 tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt
    67381 tctgataaaa tgatcgaatg ttcattgtaa taagcataag gggaatattg gaatccccag
    67441 ctttcaccgt ctagattcat tcgaataatc cggtgattgg tgcgggctgg atagttcaag
    67501 cgacctctat atccttcgtt ttccatgcaa agcatgcagg taggatagtc tacttttacc
    67561 gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg gcttagataa attgattgtg
    67621 atttccagtt caccatactc cgactctgcc ggaaatttga tgttttttgc gatggctctt
    67681 gttttgatgt agtcattttt cttacttaaa ttaaagaaat actcagttgc ttcctctggg
    67741 cttttcgaat agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa
    67801 tccattaatt gcgcttcaaa aatctcttgt tctgctggca attctgaaag aacaccattt
    67861 tctcttgcct tagcgacaag ctgatccaat aagtctacag attcttcctt aactggtctt
    67921 acttcaatgc tttctagagc atcttctcct accatcccta aaagacgatt ctgtaaatat
    67981 aagcggtcca tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc
    68041 gtttgactga ttgacatatc tcattcccct attcttcata accatttgga tggctgacat
    68101 gccaatccca tgctgttttg ataatcgctt tgatatctgt gtattcaggt ttccagccca
    68161 gaatctctct cgctttttca cttgaagcga ctaatcggct aggatctcca gctcttcttg
    68221 gtgctatttc tgctggaatt tcttttcctg tcacttctct agctgcttct agcatttcct
    68281 tgactgagta tcctttgctg ctgcctaaat tgaagaagtt gctttcattt ccttctttca
    68341 agtattccaa agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca taatctctga
    68401 tacatgttcc atctggtgta tcataatcgt ctccataaac agccaaggct tttctttgac
    68461 caagagctac ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga tcttctccga
    68521 tagatgcatc tgcttttgct ccggctacat tgaagtagcg caaagcgaca tagcgcatgc
    68581 catatgcttg gtcacaccac ttcatcattt tttccatcat caatttactt tcaccatacg
    68641 gatttttagg attagctggt gtatcttctg tgattggaga ctctttcggc tctccataag
    68701 ttgctgcagt agaagagaat acgattttat tcacgttgaa ctcgtgcatg acttcaagta
    68761 agatttgcat accgtatacg ttgttgttga aatacatcaa tggtttttcg acagattctc
    68821 ctactaaaga gcttgcagca aaatggatta ctccttcaat cggttccttt tcaaatacgg
    68881 aacgtagaaa ttctttatcc cggatgtctc cttcataaaa atgagcatct ggatgtactg
    68941 cttgtttgtg acctgtgagc aaattatcta ccacagctac ttgatacccc ttttgaacta
    69001 gctggtctac agcgtgcgaa ccgatataac cggctccgcc tagtactaaa attgtcataa
    69061 aaaacactcc tttgattagt aaaattgtgt ttgttgattc tatataattt aaaaaacgaa
    69121 gtttactttt atggtgaaaa caaacaaagt aaaatttcgt aaattcatat ggtaaattta
    69181 taataaaaca tttagtaaac attttcaagg gttttttgca aaggtttaca aacaaacgta
    69241 aaactagcct ttttttctct tttgttccat aatagatata acaaataata aatgaggtgg
    69301 attcaatgaa aaaatttgta tctggaatat tagtaggaag tctggcaaca gccgctgccg
    69361 ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag
    69421 caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg cgtttctcgt taataattat
    69481 ttaaaaatac gcatgaagac tttctaaaaa acagataagt catcatgcgt atttttattt
    69541 gtcagtcaat ataagttgtg ctattttatc tgcctgtctc agattccatg gatctgtata
    69601 aggtctagct actgctgcag ttagccaatc cacaccttca acaggtattc catagcaata
    69661 aaagtcagac tggatttccc ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc
    69721 tatagcttct gaatgataac ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt
    69781 gttttccttc aaatttttta tgacaggatt acttgaacga gaaaagcttg tttgtggcaa
    69841 tcgagcttca atcaaatagc gactgctgat ttctttatct tgtagagaaa taacatagcg
    69901 gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat
    69961 cagtgctgct aattctttcg tccgaatgat tggcggaccg atcgttaaaa agctatttag
    70021 atgagtaaac catccccata gtatttcttt gtattcctta gcagaaaaaa cgtgccattc
    70081 aatagcctgc cggattggat cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc
    70141 tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct tcaataaata ttcggctagc
    70201 ttctgaaaag ggctcattct gataggcaag aggagtttct aaatacgacc aatcccatct
    70261 ataagggatt agtgctggat gctttccgag acactcctct gaagagaggg agagaaaatc
    70321 atgctcaaaa tttttctttg gaatcggcaa gtgatattct tcgatgattt tcttgtaata
    70381 aaacaactca acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga
    70441 ctgatgccat tgctccaggt tttcttttgt tagtaaccgt ggccatgcca ttgctccgcc
    70501 ttgcttttga tttcttcctc gagggtaata agggagcccc cttcttgatc cactgtatac
    70561 aatcggttcc ttgcctgatg gatgatagac cagttttccc ttttcgttct cgaagcgccc
    70621 tcctcttgat actgtaaaca atccgacata atcaaaaaaa cttaagccta gacctcttaa
    70681 aaagacattt tctttaggcg gtatcttcga aagatctaca tctgcaggat tagaaggaag
    70741 ctgatacagt aaaacatttt tctttgcgta gtctacccac actctttctt cttctgtctc
    70801 atcattttcc cagtgaccgc ttgctaaaac aagctgatcg atagaatagc tttgctgatc
    70861 tgtctgtaaa agataaccat cttttctttt gcttactttt tttactaaag aataaacaaa
    70921 ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc
    70981 gtataaacat cgagtagatt gctgatcttt ttccagacga ttgatttctt ctataattag
    71041 ttgcttattt ttggttaccg tctgttcgat ataatctgta gcaggtcctt tactccattg
    71101 aaacaagttg ggtccctgtg ttaccaatcc tccactggac aatgtttcat ctgtaaaaag
    71161 cgtcacttgt tgagaaacag aattcattaa taactccggt ggctgattca accgccaaac
    71221 tcgacctcca ggaccattgg gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt
    71281 attatggaga atcctttctg ctacacttaa tcctctaggt ccggcaccaa tgatccctat
    71341 tttcatttgc atgcctccat ttcgataaat tttttcaata aaacagcatg gttctgatgt
    71401 tcattttttg caccataaac aaaagtaatg actggatgtt ctttcataag ttgactgagt
    71461 ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt cttgctggta tttctttgca
    71521 aatgaagcaa atcgttctgg tacatggtca aaagttttgc gtgcttcatt actaggtgca
    71581 atttctttca accacagatc taactgcgct ttttcttttg aaattcctcg tggccagaat
    71641 ctatccacga gtattctata gccatcctct ttttcagcat caaggtaaac acgttttaat
    71701 ttgatcatag ttgtttcctt ctttctacct tttcgcttag cttatatatg tagataatag
    71761 cacaaaataa aaaaaatgaa cggcacaact atccattgcg ccgttcatgt gatcttggtc
    71821 aaaatctaat gattgattcg gtttttaaag ctgtcataca atcatctgtt ttatactttt
    71881 ttacttttga tttttccaga ccatccgtca tatccaccct taagtaaata aatctcttga
    71941 taacctgctt tacgtaattt atttgctgta cggatgctca aacttttctt ttgatcgtaa
    72001 atatagacag gctggtcttt tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg
    72061 atgtttcttg cacctaaaat atgcccagca tcaaacacat ctttttcgcg aacatcgatt
    72121 acttgtgctt ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt
    72181 ttggccatta ctcgtagata gacttcccaa aggatgattg caagcacaat caatagcaaa
    72241 atgatattaa ttacccataa aaccatgtaa tgcctccgtt tttttaagca aatttcaagg
    72301 ctaatgaggc agcaccaatg acaccagctt cattaccaag ctctgctaat ttgatttttg
    72361 ttgattctct tacttgcggg aaagtaaatt ctttaaaata tttctctaca cggctacgta
    72421 aaaattctcc tgctgccgat actcctccac ctaacacgat acttgacgga tttaatgtat
    72481 tccccaagtt tccgcaagct aaacctaagt agaagcatac gcgatcaaca accatcaatg
    72541 caaacggatc gtcttcttga gccatttcaa agatatcttt acttgttact tcttctccgt
    72601 tgtctagcat tgctttcagt tttgaatccc ctgcgtattc ttctgccaag aaacgaccta
    72661 ggcgtaccac acctgtagcg ctagatacag tttctaaaca tccgcgttta ccgcaagtac
    72721 attcgaaccc ttcaggatcc acagtgatat gtccgatttc tcctccagct ccgctcacgc
    72781 cgtgaatcaa atttccgcca gcaacgatac ctccgccaac acctgttcct agagtaacga
    72841 atacaacgtc tggatcgttt tctccagcac ctttccatcg ttcacctaaa gcagcaacat
    72901 tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc ctcgatcagt tttttcacaa
    72961 attgaacttc tgtccagttt aagttatacg caccaataac cgttccagct tcgctgtcca
    73021 cgcttcctgg ggtacccatg ccgattccga taaagtcttc ggctttcata ttatataaat
    73081 tcaaacgatg attgattgat tcaatgattt caggaacgat atgcttacca tcatccaaaa
    73141 tattggtatc gatgctccat ttttgctgaa tctctccttc tggtgttaaa attgcaaatt
    73201 ttgctgttgt tccacctaaa tcaatcccaa taatcttctt gtccattaca aatttccttt
    73261 ctttcggctt tcttcaattc ggtgctctct ttttaatatg cgccacgctg tcatgtatgt
    73321 atcatgatca atcaaacggc tatcatacag attacgcaat tcgatagtca ttaattcaat
    73381 gtcatagatg cgtgcaccca catagataaa gatgccaaat tttttgaaca gctgctgtac
    73441 atcatataag ctctccatct ttaagcactt ccttcatatt atacaagtaa accatatttt
    73501 tttaaaccca gtaaaataca tattacaaaa agaaatacaa aaactagtgt agaaagaatc
    73561 ctttggtgta tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt ccctaacact
    73621 aatccgccaa ttagcccacc gacatgtccc caaacatcta cagaggtatc cagaagacca
    73681 aaaacaaatg tcaacacaat aaataaaaga aaatgtctga ctagctgctg gatcgctggg
    73741 ttatgtttaa aatgaaaccc taagataaac aacgcaccga acaaaccgaa tatcgaagta
    73801 cttgcaccac cagacagaac attcgcttcg ttaaaagcaa agcttgctgt atttcccatt
    73861 atcccgctga acatataaat caagaaaaaa cgccaatggc catagattgc ctcgatttgc
    73921 tgccccataa aatacagtat cagtgagttc attacgaaat gcatcagtcc aaaatggatg
    73981 aagattggtg tcactaaacg ccaccattca ttgaagtgaa caagataagg accatacatt
    74041 ccggcataat aagggatctc aagtcgcgga aataattcca tcatgaggaa gaccgctgtc
    74101 tgtatcccta aaaaagcata ggtccataac ggtcgtctta accatgcacg caattttatt
    74161 tgctgttgat aattcatgaa aacctcccat ttcattttag tcaataaaca attgttggat
    74221 cttctgatca aagctctccg gttgccattg ttcattcaat tgttcacgaa aaacaaggct
    74281 acatgttttt ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc caaaaccgat
    74341 tcgataaccg gctctattgt agataatacc tggtacaatg aggagatcga tcatctccgg
    74401 caaatagagc ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta caagaacgga
    74461 ctgttcatcc atttcataaa acgccatctg acgtttcggc aaagttttag gtacaactat
    74521 tttcttttgt tctttttctg ctcgttggat aaccggctgt gtatccagtt caaattcatt
    74581 cgataaagtc gtccctatca cttttgctga cttccagtac tcgctggcga atagctgttt
    74641 atagattcgc tgttcttttt cctttttctt gccacttttg gccagatttt tcagcttttc
    74701 gattcctttc cggcgtaatc ttactttcaa ttggtccaaa aactcaccct tccttctctc
    74761 ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa atgcataaat
    74821 agaataaaaa gaagacctag aaatgattgg ccttcctttt ttaatgaatt tcaactgttc
    74881 agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat atggcgcacc aaaaagggca
    74941 atcaaaattc ctgtaggtac ttccccactt ggtagaatca ctctagcaac catatctgca
    75001 ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt attttcttta
    75061 cgtacgacta gcttcgcagc atgaggagct agtagtccta caaaagataa actacctgtc
    75121 accgctacac taccgcttgc caaagcaaca gctaacaaca aaaggatttt ttgactttta
    75181 gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc
    75241 tgtgcataaa gtcctcctag cggaattaag ataaccgccc atggaagcaa tgcccaaaca
    75301 taaggccagc tcgcacccca gattgatccc gctaaccatg cattcacaaa tccataattt
    75361 tccttagata attggatcgt gcatagcaat gttaatgcag aaaggccggc attaacggca
    75421 ataccggaca ataagattcg attcataccc aaaaagccat ttcgatcata ggccgcaaga
    75481 tagacacagc aagcagctaa aacaccacct agaaaggcta caactagcaa tagaaacggc
    75541 agcgaatagt gcgaaaagaa acctaaataa agcatcacta aaaatcctgc tccggcatta
    75601 atgcccaata tactagaatc tgccaattca tttcttgtta ctccttgcaa gatataacca
    75661 gaaacgccaa gacaagctcc tgctaataaa ctgatcagta atcgaggaac tcgaaattct
    75721 ctaacaatca gcgccaaaga agatgattct ttccctatga acgtggcaaa aagctgatcc
    75781 aggtaaataa aaggcgtacc aatcattaaa ctacctatag acaggatgac aagtacaaaa
    75841 agcaagaaat agaacgtcca atatattttt ttcaccgttg ttccctcctt acttgtatca
    75901 gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa aggtgtttca aaagggggat
    75961 tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc accgccaact aaaaaagaga
    76021 caggtagaac ataacgatag tccgttccaa ccattccgcg aacaactggc ggaaccaaca
    76081 gcccaataaa tgaaacaggt ccgactaacg aaacggcaat ggctgctaac actgcaacta
    76141 gacagaaaac aatcaaccgc gttcgtttag gatgacttcc aagagctaat gcatgtgttt
    76201 cactcaaatg aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa acagcaagta
    76261 tatataaagg aaacaatact gctaattgtt tccatgtgat attggcagct ccgcctacaa
    76321 accaaaaagc aagatcctgc tgcagattaa agaattgggt aagtgcttgg ctgatagcag
    76381 taaaaaaaga actaatagct acgccagcta aaatcagctg taaaggattc atgcccattt
    76441 tcgaaaactg caaaaaacca aatacgagca aagttacagc aaaagaacct gccaatgaca
    76501 ttagaaacac agaaaaatca gaagctttag gccaaaaagc aaaactaagg gccattgcca
    76561 acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc taaaggattt cttgtcaccc
    76621 cttgcatcaa tgcaccgctc aatgcaaaac aactccctac aagaaatgct cccgccacac
    76681 gaggcaaacg aatatttcgt atgatttggt gactttgttt tgatctatca aaatcaaaca
    76741 acgcctgcca aaaatctgct ttttccacag tcgctgcgcc atttgaaaga gcaacaaaaa
    76801 aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct atttttcttc atagtcctca
    76861 taattgatcc tcctgctctt ttggcaacaa atcataggtt agaaaaactg gtttatttgt
    76921 tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac actcttttgt
    76981 gatcacttct tcaggtgtac cttctgtgat caacttaccg tttttcattg ctaataaata
    77041 atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac atcaagatcg ttgtttgttt
    77101 catttgattg atttcagtca ataactgtag gatctctaat tggtgtgccg gatctaaaaa
    77161 tgttgtcggt tcatccaaga tcaagatatc tgtttcctga gcaagcgcca tagcgatcca
    77221 gacacgctgg gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa atccagctag
    77281 acctgtagca tccaacgccc actcaattgc ttcatgatct tgcgtgtcca tgccactgaa
    77341 cagtttttga taagggtatc gtccgtaaga tactacttct tctactgtca tacctgtagg
    77401 gtgttcacta gtttgtgcta ataatgcaat tttttttgca agttcttttg aactgtatgt
    77461 agagatatca gagttattga caatgatctt tcctgatttc acaggtagta tacgggtaaa
    77521 acttttcaat aaagtagatt ttccacttcc atttggccca atcaaactag tgattttccc
    77581 ttcaggaatc gtaacagaca tgttttcaat gatttttttg ttttcgtagc cggctgcaat
    77641 gtcagctgct cttaatctcg tcatttcacc catcctttcg ctgaaaatca ttctcaatta
    77701 tgacgctgtt tcaacatttc gtcaagcgct actttcatat ctatagccaa atgaagcggt
    77761 tgacagcgtt cgaaatatca ggaataataa ccaatgagaa agattctcaa ttatgttagg
    77821 aggaaagaaa tgaaattaaa aacaaaagta ttcacattat ctagcatatt gctgctggct
    77881 ggagtactga gcgcttgctc cccctcttca gataaagaaa cagctacaac tgccaattcg
    77941 acgacttcat ccaatagtac tcatacagtc acagatacac ttggacataa agtagagatt
    78001 ccaaacgcgc ccaaacggat catcggcagt tacttagaag actatctgat tgcactaggg
    78061 gaaaaaccta tcgcccagtg gactgttggc agtggttcca ttcaagatta tctgcaagat
    78121 gatttaaagg atgtcccaac gatttcttat gaccttcctt atgagaaagt gttaagcttt
    78181 gagcctgatt tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa
    78241 tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac atgggaagaa
    78301 caactaaaag atgtcggcaa agctttggca aaagaaaaag aggcagaaaa aatcatttcc
    78361 gattatcaaa aatccatcaa gaaaactcga gaagaactag cagataaaat cgagaacaag
    78421 acagctgcag tgttgtgggt gaccaacaac tcagccttta tggtcagcaa aaatcggtca
    78481 agtggaagaa tcgtatacga cgatctggaa tttggtgttc ctgatctagt ggaagaggtc
    78541 tccaaaaaag ccacttctga ctggtctgct gtttcctctg aaaagctagc tgaattagat
    78601 gctgactaca tcatccttgt gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc
    78661 tggaagaatc taaaagcagt gacagacaat catgtactgc aattcggacc tgaatctagt
    78721 tggctatata acggacctat tgccagtcag gaaatggtca atgatatcaa agaaaaacta
    78781 aattgatttt aactaaaaaa agcatcttca cgattagtcg tgagatgctt tttttcttat
    78841 taacaaacta gacgcataga ttttattctt cttttgaagc aaccctattt tttactcttt
    78901 cccatttttg aaaacgatgg gcatatagat tttcttgatc cgttatcccc tcatactcct
    78961 ctataagggc gaagtctgaa aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc
    79021 tatcgatcaa tgttcgccag atccgaccag tgtaaggaag aagttcttga aaaatccgac
    79081 taccacccga aacatagata tcttctgaga tcgtttgact atacgacaaa agttcatcga
    79141 tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga
    79201 tgatatggcg ataaggaagt gaaagatccc ccattccttc ataggtcttt cttcccatca
    79261 ctaaaattcg atccattgta tgttcgcgaa agaaacgcat atcatttggc aatcgccatg
    79321 gtaacaaacc atttttgccg atcagaccat ttttatcttg tgcccacatg gaaataaaca
    79381 tattatccgc tccttatgat tttagatgtt ttgagataaa aaaagtctca attctttgac
    79441 cagcaaggaa ttgagaactt taaaaacatt attttgtttc acggtgcaaa gtaactttgc
    79501 gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg attgttacgt ttgtttttgc
    79561 ttgttagata gttacgttct ttacaagaag tacactctaa agtgatgttt acgcgcatgt
    79621 ttttccctcc catttttaca agaatcctct aagatgactc tcagacttga ttatcttacc
    79681 atgatttttt ctagattgct agtgtttttc acgattttgt taagtaaaac tacttgacga
    79741 taaatacaac ggttttactt ctcaaacaaa atctttttct ttttgtataa atacccttac
    79801 acttgtttat atagattatg ttttttaata aagggggata ctaatatgcc atggaacatg
    79861 aaggactttc ctgcatcaat gaaaaactta gataaattga caagaaaaaa agcaatcgac
    79921 atcgcaaatg cattattaga tgaagggtac cctgataacc gtgcgatccc aattgccatt
    79981 gatcaagcaa aaaaatggta tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa
    80041 gaaaaaaatc caagcaagac ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt
    80101 gattcagatg tgatcgtaga atatgaggaa gaacaatgga tcgtgaaatc taaaggggca
    80161 aaaaaggcaa gcaaccattt tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta
    80221 gcacaaaaca aagaatcaac tttgttcatt tataaaaaag atgggacaaa agaaaaagaa
    80281 atcgattatt gaacatatgt aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg
    80341 ttcgaaacaa tggtgttcct ctgccaactc cgaaccataa gtcataatat ctataaaacg
    80401 ggaagagctg ttttttgaaa ttgtttctta tcactcagtg ttaaacggta ggagaaaacc
    80461 tcttttttat tgatatttga ttttcatcaa acagtcacat agtcgattgc ttctgcttgc
    80521 ggaatcgttt catctacaaa gtagacttta taccatgtca agaaactgaa gattgtccag
    80581 atttttcttt gctcttcttt acgtccttcg tgatggtcat ctagcaactt catgattttt
    80641 tcttgatcaa aaaattcttt tgcaaaatct gcttcgaata atgctttgac ttgttcgtag
    80701 ccgtgttctt ctttcaacca agctttgatt ggcacaggga atcctaattt cacacgattc
    80761 gaccattctt ccggaagatg tttgttcgca gcttgtctga aaatatcttt tgtgttgttt
    80821 tgattcaata aaaacttact tggtatttgt tgagccaatt tcatgacttc gatatccaat
    80881 aaaggtactc ggacttccaa agaacttgcc atagaaagtt tatctgcctt taacaagata
    80941 tcttttggca tccattgatg aagatcgaca tattgcatct tattgacttc atctttgatt
    81001 ccttctgttt tctcgtaatg aacagtcatg atttcatcca cagatggtgc tgtttgatat
    81061 tttggtgtta acacttctga tgcttctgat tcttcgaaca ctcgagcatg accgataaaa
    81121 tattcttttg caggagcaag tgattcatat aaatggatcc gtccatggaa gtttttcatt
    81181 tttccgattt tacgtcctaa attatagcgt gttccttttg gtaatttttt caatccttga
    81241 gcaatcacac ggatgaattt tgaattcgta tggaatccat acgtttggta tcccgcaaac
    81301 agttcatccg ccccttctcc agattggact acccgcacac tttgtgaagc aagatttgct
    81361 aaaaagtaca aaggtacgca agaaggattt gaatcaggtt catctaagtg atactggatc
    81421 aaagggaatg ctttgaacga catgtctccg tcgatcactg ctgctgtatt atctaagtcc
    81481 aatagttcgg ttagttttct ggcttcaatc gcttcattat aggttttatc atcaaacccg
    81541 attgagaaag aatgatctgg tttcaataca gaggttacat aactggaatc aaccccgctg
    81601 gataagaaag aaccaacttc tacatcactg accgtatgtg cttcaataga agcttgaacg
    81661 gtttcgtcga ttttttcgat ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct
    81721 gcatcccaat attgctgaat gtctattttt ccatctttat acgtataata atggccttca
    81781 ggcaaacgat agacatcttt gaagaatgtt tcgccgttta atggagaata ttggaaagtc
    81841 atataaggtt tcaacgcttc tttgttcaat tctttgttaa aatcaggatg aggcagaaaa
    81901 cttttgattt ctgaaccaaa cataaatgtt ccgttcattt ctgcataata atatggtttg
    81961 atcccaaagt ggtctcttgc accgaataac tcattctttt cattatccca aatagcaaaa
    82021 gcaaacattc cgcggatctt ttgcaataat tctgttcccc attcttcgta accatgcaaa
    82081 aggacttctg tatctgcatg tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc
    82141 aaaggctggt aattatagat ttcgccatta aaaataatga tctttgtccg atcttcattg
    82201 taaataggct gagtaccgcc ctctaagtca ataatactta atcttctgaa tcctaaagca
    82261 acatgtttat cgatatattc tcctgaacta ttaggtccac ggtgaacgat tcgatccatc
    82321 attgtattaa taattgtttt tttgttgtcc ttgtcgttaa caaaacctac aataccgcac
    82381 atttaacttt cacatcctaa tataaaatta ctgtttattt cattcatatg catcttatca
    82441 tagaataaat agcggacgaa tattttctct cttacaattt cgtcacgtga aaacaaaaga
    82501 tgaaagtttc tgtttttaca gaaaataaag tcccaatatc taatttacca tgttttcttt
    82561 tttgaggcta ccttattctc ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa
    82621 aaccagcaaa aaaagacacg acctaaagat cgtgt
//
LOCUS       DACDKJ010000006        74207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDKJ010000006 DACDKJ010000000
VERSION     DACDKJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.006"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..562
                     /locus_tag="IV328_000449"
     CDS             <1..562
                     /locus_tag="IV328_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8936618.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="IV328_000450"
     CDS             762..1076
                     /locus_tag="IV328_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8936619.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="IV328_000451"
     CDS             1097..1474
                     /locus_tag="IV328_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8936620.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="IV328_000452"
     CDS             1484..2257
                     /locus_tag="IV328_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936621.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="IV328_000453"
     CDS             2279..3682
                     /locus_tag="IV328_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8936622.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="IV328_000454"
     CDS             3864..5048
                     /locus_tag="IV328_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8936623.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="IV328_000455"
     CDS             5045..5335
                     /locus_tag="IV328_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936624.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="IV328_000456"
     CDS             5332..5553
                     /locus_tag="IV328_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936625.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="IV328_000457"
     CDS             5595..6374
                     /locus_tag="IV328_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP8936626.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="IV328_000458"
     CDS             6441..6935
                     /locus_tag="IV328_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8936627.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="IV328_000459"
     CDS             6991..7629
                     /locus_tag="IV328_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936628.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="IV328_000460"
     CDS             7701..8096
                     /locus_tag="IV328_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8936629.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="IV328_000461"
     CDS             8080..10533
                     /locus_tag="IV328_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8936630.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="IV328_000462"
     CDS             10530..12557
                     /locus_tag="IV328_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936631.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="IV328_000463"
     CDS             12554..13576
                     /locus_tag="IV328_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8936632.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="IV328_000464"
     CDS             13593..14522
                     /locus_tag="IV328_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8936633.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="IV328_000465"
     CDS             14767..14883
                     /locus_tag="IV328_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8936634.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="IV328_000466"
     CDS             14899..15702
                     /locus_tag="IV328_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8936635.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15877..16173
                     /locus_tag="IV328_000467"
     CDS             15877..16173
                     /locus_tag="IV328_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936636.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="IV328_000468"
     CDS             16180..18132
                     /locus_tag="IV328_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8936637.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="IV328_000469"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="IV328_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8936638.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19063..20103
                     /locus_tag="IV328_000470"
     CDS             19063..20103
                     /locus_tag="IV328_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8936639.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20201..20389
                     /locus_tag="IV328_000471"
     CDS             20201..20389
                     /locus_tag="IV328_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8936640.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20447..20800)
                     /locus_tag="IV328_000472"
     CDS             complement(20447..20800)
                     /locus_tag="IV328_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8936641.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21331..21753
                     /locus_tag="IV328_000473"
     CDS             21331..21753
                     /locus_tag="IV328_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8936642.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21750..21980
                     /locus_tag="IV328_000474"
     CDS             21750..21980
                     /locus_tag="IV328_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8936643.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22478..22678
                     /locus_tag="IV328_000475"
     CDS             22478..22678
                     /locus_tag="IV328_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8936644.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22705..23898
                     /locus_tag="IV328_000476"
     CDS             22705..23898
                     /locus_tag="IV328_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8936645.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="IV328_000477"
     CDS             complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="IV328_000477"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8936646.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25590..26513)
                     /locus_tag="IV328_000478"
     CDS             complement(25590..26513)
                     /locus_tag="IV328_000478"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8936647.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26709..27314
                     /locus_tag="IV328_000479"
     CDS             26709..27314
                     /locus_tag="IV328_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8936648.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27622..28638
                     /locus_tag="IV328_000480"
     CDS             27622..28638
                     /locus_tag="IV328_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8936649.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28779..30080
                     /locus_tag="IV328_000481"
     CDS             28779..30080
                     /locus_tag="IV328_000481"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8936650.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30126..30788
                     /gene="deoC"
                     /locus_tag="IV328_000482"
     CDS             30126..30788
                     /gene="deoC"
                     /locus_tag="IV328_000482"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8936651.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30809..31207
                     /locus_tag="IV328_000483"
     CDS             30809..31207
                     /locus_tag="IV328_000483"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8936652.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31273..32355
                     /locus_tag="IV328_000484"
     CDS             31273..32355
                     /locus_tag="IV328_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8936653.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32567..34129
                     /locus_tag="IV328_000485"
     CDS             32567..34129
                     /locus_tag="IV328_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936654.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34122..35255
                     /locus_tag="IV328_000486"
     CDS             34122..35255
                     /locus_tag="IV328_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8936655.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35255..36211
                     /locus_tag="IV328_000487"
     CDS             35255..36211
                     /locus_tag="IV328_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8936656.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36310..37476
                     /gene="deoB"
                     /locus_tag="IV328_000488"
     CDS             36310..37476
                     /gene="deoB"
                     /locus_tag="IV328_000488"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8936657.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37500..38318
                     /locus_tag="IV328_000489"
     CDS             37500..38318
                     /locus_tag="IV328_000489"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8936658.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38335..39042
                     /gene="deoD"
                     /locus_tag="IV328_000490"
     CDS             38335..39042
                     /gene="deoD"
                     /locus_tag="IV328_000490"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8936659.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="IV328_000491"
     CDS             complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="IV328_000491"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8936660.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40198..40779)
                     /locus_tag="IV328_000492"
     CDS             complement(40198..40779)
                     /locus_tag="IV328_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8936661.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="IV328_000493"
     CDS             complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="IV328_000493"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8936662.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41914..42327
                     /gene="rpsL"
                     /locus_tag="IV328_000494"
     CDS             41914..42327
                     /gene="rpsL"
                     /locus_tag="IV328_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8936663.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42411..42881
                     /gene="rpsG"
                     /locus_tag="IV328_000495"
     CDS             42411..42881
                     /gene="rpsG"
                     /locus_tag="IV328_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8936664.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42975..45062
                     /gene="fusA"
                     /locus_tag="IV328_000496"
     CDS             42975..45062
                     /gene="fusA"
                     /locus_tag="IV328_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8936665.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45234..46421
                     /gene="tuf"
                     /locus_tag="IV328_000497"
     CDS             45234..46421
                     /gene="tuf"
                     /locus_tag="IV328_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8936666.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46828..47136
                     /gene="rpsJ"
                     /locus_tag="IV328_000498"
     CDS             46828..47136
                     /gene="rpsJ"
                     /locus_tag="IV328_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8936667.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47167..47796
                     /gene="rplC"
                     /locus_tag="IV328_000499"
     CDS             47167..47796
                     /gene="rplC"
                     /locus_tag="IV328_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8936668.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47824..48447
                     /gene="rplD"
                     /locus_tag="IV328_000500"
     CDS             47824..48447
                     /gene="rplD"
                     /locus_tag="IV328_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8936669.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48447..48737
                     /gene="rplW"
                     /locus_tag="IV328_000501"
     CDS             48447..48737
                     /gene="rplW"
                     /locus_tag="IV328_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8936670.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48776..49609
                     /gene="rplB"
                     /locus_tag="IV328_000502"
     CDS             48776..49609
                     /gene="rplB"
                     /locus_tag="IV328_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8936671.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49652..49930
                     /gene="rpsS"
                     /locus_tag="IV328_000503"
     CDS             49652..49930
                     /gene="rpsS"
                     /locus_tag="IV328_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8936672.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49953..50300
                     /gene="rplV"
                     /locus_tag="IV328_000504"
     CDS             49953..50300
                     /gene="rplV"
                     /locus_tag="IV328_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8936673.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50314..50970
                     /gene="rpsC"
                     /locus_tag="IV328_000505"
     CDS             50314..50970
                     /gene="rpsC"
                     /locus_tag="IV328_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8936674.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50973..51407
                     /gene="rplP"
                     /locus_tag="IV328_000506"
     CDS             50973..51407
                     /gene="rplP"
                     /locus_tag="IV328_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8936675.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51397..51585
                     /gene="rpmC"
                     /locus_tag="IV328_000507"
     CDS             51397..51585
                     /gene="rpmC"
                     /locus_tag="IV328_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8936676.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51610..51876
                     /gene="rpsQ"
                     /locus_tag="IV328_000508"
     CDS             51610..51876
                     /gene="rpsQ"
                     /locus_tag="IV328_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8936677.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51934..52302
                     /gene="rplN"
                     /locus_tag="IV328_000509"
     CDS             51934..52302
                     /gene="rplN"
                     /locus_tag="IV328_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8936678.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52339..52647
                     /gene="rplX"
                     /locus_tag="IV328_000510"
     CDS             52339..52647
                     /gene="rplX"
                     /locus_tag="IV328_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8936679.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52675..53214
                     /gene="rplE"
                     /locus_tag="IV328_000511"
     CDS             52675..53214
                     /gene="rplE"
                     /locus_tag="IV328_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8936680.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53233..53418
                     /locus_tag="IV328_000512"
     CDS             53233..53418
                     /locus_tag="IV328_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8936681.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53454..53852
                     /gene="rpsH"
                     /locus_tag="IV328_000513"
     CDS             53454..53852
                     /gene="rpsH"
                     /locus_tag="IV328_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8936682.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53884..54420
                     /gene="rplF"
                     /locus_tag="IV328_000514"
     CDS             53884..54420
                     /gene="rplF"
                     /locus_tag="IV328_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8936683.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54581..54937
                     /gene="rplR"
                     /locus_tag="IV328_000515"
     CDS             54581..54937
                     /gene="rplR"
                     /locus_tag="IV328_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8936684.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54958..55458
                     /gene="rpsE"
                     /locus_tag="IV328_000516"
     CDS             54958..55458
                     /gene="rpsE"
                     /locus_tag="IV328_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8936685.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55473..55652
                     /gene="rpmD"
                     /locus_tag="IV328_000517"
     CDS             55473..55652
                     /gene="rpmD"
                     /locus_tag="IV328_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8936686.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55696..56136
                     /gene="rplO"
                     /locus_tag="IV328_000518"
     CDS             55696..56136
                     /gene="rplO"
                     /locus_tag="IV328_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8936687.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56136..57431
                     /gene="secY"
                     /locus_tag="IV328_000519"
     CDS             56136..57431
                     /gene="secY"
                     /locus_tag="IV328_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8936688.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57493..58140
                     /locus_tag="IV328_000520"
     CDS             57493..58140
                     /locus_tag="IV328_000520"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8936689.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58331..58549
                     /gene="infA"
                     /locus_tag="IV328_000521"
     CDS             58331..58549
                     /gene="infA"
                     /locus_tag="IV328_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8936690.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58582..58698
                     /gene="rpmJ"
                     /locus_tag="IV328_000522"
     CDS             58582..58698
                     /gene="rpmJ"
                     /locus_tag="IV328_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8936691.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58716..59081
                     /gene="rpsM"
                     /locus_tag="IV328_000523"
     CDS             58716..59081
                     /gene="rpsM"
                     /locus_tag="IV328_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8936692.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59109..59498
                     /gene="rpsK"
                     /locus_tag="IV328_000524"
     CDS             59109..59498
                     /gene="rpsK"
                     /locus_tag="IV328_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8936693.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59578..60516
                     /locus_tag="IV328_000525"
     CDS             59578..60516
                     /locus_tag="IV328_000525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8936694.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60544..60924
                     /gene="rplQ"
                     /locus_tag="IV328_000526"
     CDS             60544..60924
                     /gene="rplQ"
                     /locus_tag="IV328_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8936695.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61196..62035
                     /locus_tag="IV328_000527"
     CDS             61196..62035
                     /locus_tag="IV328_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8936696.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62011..62880
                     /locus_tag="IV328_000528"
     CDS             62011..62880
                     /locus_tag="IV328_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8936697.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62870..63667
                     /locus_tag="IV328_000529"
     CDS             62870..63667
                     /locus_tag="IV328_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8936698.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63870..64616
                     /gene="truA"
                     /locus_tag="IV328_000530"
     CDS             63870..64616
                     /gene="truA"
                     /locus_tag="IV328_000530"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8936699.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64949..67618)
                     /locus_tag="IV328_000531"
     CDS             complement(64949..67618)
                     /locus_tag="IV328_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8936700.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67856..68044)
                     /locus_tag="IV328_000532"
     CDS             complement(67856..68044)
                     /locus_tag="IV328_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8936701.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68314..68676
                     /locus_tag="IV328_000533"
     CDS             68314..68676
                     /locus_tag="IV328_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8936702.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68728..70410
                     /locus_tag="IV328_000534"
     CDS             68728..70410
                     /locus_tag="IV328_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8936703.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70527..72563
                     /gene="recG"
                     /locus_tag="IV328_000535"
     CDS             70527..72563
                     /gene="recG"
                     /locus_tag="IV328_000535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8936704.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72612..73613
                     /gene="plsX"
                     /locus_tag="IV328_000536"
     CDS             72612..73613
                     /gene="plsX"
                     /locus_tag="IV328_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8936705.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73735..73977
                     /gene="acpP"
                     /locus_tag="IV328_000537"
     CDS             73735..73977
                     /gene="acpP"
                     /locus_tag="IV328_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8936706.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        24417 a        13186 c        15529 g        21075 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    20161 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    20221 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20281 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20341 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20401 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20461 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20521 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20581 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20641 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20701 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20761 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20821 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
    20881 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
    20941 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
    21001 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
    21061 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
    21121 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
    21181 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
    21241 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
    21301 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
    21361 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
    21421 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
    21481 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
    21541 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
    21601 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
    21661 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
    21721 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
    21781 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
    21841 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
    21901 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
    21961 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
    22021 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
    22081 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
    22141 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
    22201 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
    22261 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
    22321 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
    22381 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
    22441 ggagttttcc tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga
    22501 tttagttgag ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga
    22561 attgatgatt acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg
    22621 tgaagctgtt gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt
    22681 tttgcacagt aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga
    22741 acctattatt ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat
    22801 cgtagtggat tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg
    22861 aaaccagaag aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa
    22921 attttccttc catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca
    22981 actattaaga agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt
    23041 gatgtgcaaa attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct
    23101 gttcaaggac tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca
    23161 aatccatcta agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca
    23221 aaagaagaat ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt
    23281 ttatttatct ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc
    23341 attcaatggg aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat
    23401 tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat
    23461 attgtattag ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat
    23521 ctaattcaga cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta
    23581 tcatatgcta ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg
    23641 ttacggcatt ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa
    23701 gatcgtctag gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct
    23761 aacagtaatt tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa
    23821 caaaattatg atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa
    23881 atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat
    23941 aagaggattt ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag
    24001 tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt
    24061 tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta
    24121 cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc
    24181 ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga
    24241 tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt
    24301 cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat
    24361 caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg
    24421 gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga
    24481 tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta
    24541 ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga
    24601 attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta
    24661 agaagcgatc tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt
    24721 ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca
    24781 attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg
    24841 aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat
    24901 cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc
    24961 gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa
    25021 aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt
    25081 caaagccttc aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt
    25141 ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat
    25201 attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac
    25261 catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg
    25321 cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg
    25381 cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac
    25441 gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt
    25501 caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta
    25561 tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg
    25621 atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat
    25681 tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct
    25741 tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc
    25801 agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca
    25861 tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc
    25921 gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga
    25981 atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt
    26041 acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg
    26101 tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc
    26161 agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt
    26221 gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg
    26281 tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga
    26341 taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca
    26401 ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa
    26461 aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc
    26521 tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact
    26581 taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt
    26641 cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga
    26701 tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac
    26761 aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt
    26821 cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt
    26881 tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt
    26941 tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc
    27001 tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata
    27061 tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg
    27121 ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg
    27181 gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg
    27241 aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa
    27301 gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc
    27361 atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt
    27421 ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag
    27481 atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa
    27541 catactttcc ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt
    27601 agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc
    27661 gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag
    27721 acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct
    27781 tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat
    27841 ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg
    27901 atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt
    27961 tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat
    28021 gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat
    28081 cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg
    28141 ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat
    28201 caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt
    28261 aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa
    28321 aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc
    28381 ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata
    28441 ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag
    28501 ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct
    28561 caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa
    28621 gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt
    28681 tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat
    28741 aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg
    28801 aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact
    28861 atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc
    28921 aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg
    28981 tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg
    29041 gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta
    29101 aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc
    29161 ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag
    29221 tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc
    29281 gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa
    29341 aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca
    29401 tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg
    29461 ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg
    29521 gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc
    29581 tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga
    29641 caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa
    29701 aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac
    29761 gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa
    29821 aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca
    29881 aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg
    29941 tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca
    30001 ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc
    30061 atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa
    30121 aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag
    30181 aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca
    30241 atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta
    30301 cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag
    30361 atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat
    30421 caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc
    30481 gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag
    30541 cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa
    30601 caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg
    30661 gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg
    30721 gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag
    30781 gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac
    30841 agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg
    30901 tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg
    30961 attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac
    31021 atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg
    31081 tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa
    31141 tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga
    31201 tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga
    31261 ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca
    31321 gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc
    31381 tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca
    31441 ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga
    31501 ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt
    31561 gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca
    31621 gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc
    31681 atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt
    31741 gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta
    31801 aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg
    31861 aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca
    31921 gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat
    31981 gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc
    32041 gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca
    32101 ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta
    32161 gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt
    32221 tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa
    32281 aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact
    32341 cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg
    32401 gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg
    32461 gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt
    32521 agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa
    32581 tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa
    32641 catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg
    32701 caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt
    32761 gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg
    32821 gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt
    32881 gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca
    32941 aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc
    33001 agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact
    33061 gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat
    33121 catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga
    33181 aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt
    33241 caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc
    33301 attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct
    33361 agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc
    33421 tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc
    33481 tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac
    33541 taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca
    33601 taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca
    33661 tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag
    33721 agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt
    33781 atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct
    33841 gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa
    33901 acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga
    33961 agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat
    34021 cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt
    34081 agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa
    34141 aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga
    34201 tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag
    34261 cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta
    34321 cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac
    34381 aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa
    34441 aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg
    34501 ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga
    34561 tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct
    34621 atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat
    34681 ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg
    34741 cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg
    34801 ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt
    34861 tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa
    34921 cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg
    34981 tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta
    35041 ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca
    35101 atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca
    35161 tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac
    35221 caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt
    35281 gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac
    35341 tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc
    35401 ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg
    35461 gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc
    35521 aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc
    35581 tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac
    35641 gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat
    35701 tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg
    35761 ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca
    35821 agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc
    35881 tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc
    35941 agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg
    36001 gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat
    36061 cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc
    36121 atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc
    36181 aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac
    36241 gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa
    36301 ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa
    36361 gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa
    36421 gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt
    36481 gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct
    36541 gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca
    36601 tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct
    36661 ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt
    36721 gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag
    36781 atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt
    36841 cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg
    36901 ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc
    36961 ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag
    37021 atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg
    37081 gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc
    37141 aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat
    37201 gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac
    37261 ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg
    37321 cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct
    37381 caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact
    37441 gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa
    37501 tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa
    37561 aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg
    37621 aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg
    37681 ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag
    37741 ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga
    37801 tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat
    37861 catttgtacc aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc
    37921 ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt
    37981 atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta
    38041 aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa
    38101 tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag
    38161 cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta
    38221 tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt
    38281 ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt
    38341 gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct
    38401 ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta
    38461 cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca
    38521 ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta
    38581 aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat
    38641 ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc
    38701 tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa
    38761 gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat
    38821 gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca
    38881 gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt
    38941 agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat
    39001 gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct
    39061 gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg
    39121 ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct
    39181 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    39241 tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact
    39301 gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc
    39361 aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc
    39421 catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc
    39481 atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca
    39541 gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt
    39601 gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa
    39661 tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc
    39721 agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca
    39781 tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt
    39841 gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat
    39901 tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca
    39961 accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt
    40021 tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt
    40081 tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa
    40141 acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta
    40201 gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac
    40261 tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg
    40321 tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt
    40381 acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc
    40441 taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc
    40501 aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg
    40561 cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc
    40621 agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc
    40681 atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac
    40741 gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg
    40801 attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt
    40861 ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg
    40921 acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat
    40981 gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct
    41041 ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt
    41101 taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat
    41161 atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag
    41221 tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac
    41281 tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat
    41341 ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg
    41401 tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg
    41461 taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc
    41521 ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa
    41581 ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac
    41641 tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt
    41701 caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg
    41761 ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa
    41821 ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac
    41881 tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt
    41941 aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac
    42001 agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt
    42061 gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt
    42121 ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa
    42181 cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat
    42241 atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa
    42301 tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta
    42361 ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta
    42421 aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa
    42481 ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct
    42541 ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac
    42601 aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa
    42661 actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg
    42721 tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa
    42781 tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa
    42841 tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg
    42901 gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag
    42961 gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca
    43021 tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta
    43081 aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag
    43141 aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc
    43201 gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat
    43261 tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa
    43321 ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca
    43381 aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc
    43441 aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta
    43501 tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag
    43561 aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg
    43621 aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca
    43681 ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag
    43741 tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg
    43801 attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg
    43861 aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta
    43921 tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat
    43981 caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac
    44041 agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg
    44101 ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta
    44161 ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa
    44221 aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt
    44281 tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc
    44341 acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg
    44401 cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg
    44461 tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg
    44521 aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat
    44581 acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct
    44641 atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa
    44701 atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta
    44761 atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag
    44821 gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg
    44881 ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta
    44941 caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg
    45001 aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat
    45061 aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat atttttaata
    45121 tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat
    45181 atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa
    45241 aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc
    45301 atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc
    45361 aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa
    45421 tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc
    45481 caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta
    45541 tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt
    45601 ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg
    45661 acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat
    45721 tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg
    45781 cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc
    45841 cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg
    45901 gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag
    45961 ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt
    46021 tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg
    46081 ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc
    46141 atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc
    46201 cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg
    46261 ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg
    46321 aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac
    46381 gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt
    46441 aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga
    46501 gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca
    46561 cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca
    46621 agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta
    46681 gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca
    46741 cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt
    46801 ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa
    46861 gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga
    46921 actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc
    46981 atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa
    47041 cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac
    47101 ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt
    47161 gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa
    47221 tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg
    47281 aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa
    47341 gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc
    47401 ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca
    47461 gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga
    47521 ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt
    47581 taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa
    47641 cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt
    47701 gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct
    47761 ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa
    47821 ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt
    47881 aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg
    47941 caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc
    48001 ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc
    48061 tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac
    48121 aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct
    48181 gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc
    48241 aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt
    48301 aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat
    48361 aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt
    48421 actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga
    48481 tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc
    48541 gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa
    48601 acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata
    48661 caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat
    48721 tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc
    48781 gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc
    48841 tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg
    48901 acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata
    48961 tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga
    49021 atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc
    49081 ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc
    49141 agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca
    49201 caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc
    49261 tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg
    49321 tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt
    49381 aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg
    49441 tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg
    49501 acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa
    49561 aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc
    49621 atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt
    49681 tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt
    49741 tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc
    49801 agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa
    49861 attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac
    49921 gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag
    49981 caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca
    50041 gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg
    50101 gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag
    50161 atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt
    50221 tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg
    50281 tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga
    50341 atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct
    50401 gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct
    50461 gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca
    50521 gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa
    50581 ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat
    50641 ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc
    50701 cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa
    50761 actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa
    50821 ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat
    50881 acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact
    50941 aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc
    51001 gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg
    51061 gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg
    51121 ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc
    51181 ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac
    51241 ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc
    51301 ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta
    51361 agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa
    51421 ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt
    51481 agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa
    51541 tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc
    51601 attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca
    51661 gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat
    51721 ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa
    51781 gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt
    51841 ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga
    51901 ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa
    51961 gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt
    52021 cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt
    52081 ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct
    52141 cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat
    52201 gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac
    52261 aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa
    52321 aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga
    52381 aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt
    52441 gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt
    52501 ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact
    52561 ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa
    52621 aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac
    52681 cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat
    52741 agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat
    52801 gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt
    52861 caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga
    52921 atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa
    52981 ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt
    53041 gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat
    53101 tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct
    53161 gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag
    53221 gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt
    53281 tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag
    53341 ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggtgtg
    53401 aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca
    53461 tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg
    53521 agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg
    53581 aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt
    53641 tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac
    53701 caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta
    53761 tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata
    53821 tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt
    53881 taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa
    53941 gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca
    54001 gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa
    54061 gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc
    54121 agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa
    54181 ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct
    54241 ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa
    54301 gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt
    54361 aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa
    54421 tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac
    54481 cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg
    54541 cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa
    54601 gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga
    54661 gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga
    54721 cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac
    54781 aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg
    54841 cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt
    54901 agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg
    54961 gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta
    55021 acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac
    55081 aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt
    55141 aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc
    55201 ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa
    55261 ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca
    55321 gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt
    55381 gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt
    55441 gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac
    55501 gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa
    55561 agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg
    55621 tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa
    55681 ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta
    55741 cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa
    55801 aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca
    55861 ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca
    55921 gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct
    55981 ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg
    56041 gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca
    56101 attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt
    56161 ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc
    56221 gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt
    56281 ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca
    56341 ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa
    56401 tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac
    56461 tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca
    56521 ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt
    56581 acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac
    56641 agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt
    56701 ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt
    56761 cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg
    56821 tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac
    56881 gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta
    56941 ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga
    57001 gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata
    57061 cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct
    57121 ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac
    57181 caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa
    57241 gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg
    57301 tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg
    57361 tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat
    57421 tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta
    57481 ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa
    57541 gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc
    57601 gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt
    57661 gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat
    57721 acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg
    57781 gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt
    57841 gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact
    57901 taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat
    57961 gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac
    58021 atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt
    58081 gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag
    58141 cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat
    58201 gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt
    58261 gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga
    58321 ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt
    58381 gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc
    58441 aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt
    58501 gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt
    58561 aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat
    58621 tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat
    58681 aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata
    58741 ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta
    58801 ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa
    58861 cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc
    58921 ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa
    58981 tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc
    59041 gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt
    59101 aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag
    59161 caggtattgc acatatccat tctacattca ataacactat catcatgatc actgatactc
    59221 acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat
    59281 caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg
    59341 gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc
    59401 gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc
    59461 ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt
    59521 ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg
    59581 attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc
    59641 atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt
    59701 ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat
    59761 gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa
    59821 ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga
    59881 cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa
    59941 gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa
    60001 cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt
    60061 cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca
    60121 cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca
    60181 attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt
    60241 tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca
    60301 caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac
    60361 aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct
    60421 gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg
    60481 cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc
    60541 aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat
    60601 ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa
    60661 gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt
    60721 cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac
    60781 atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa
    60841 cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca
    60901 atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt
    60961 ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag
    61021 cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc
    61081 tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa
    61141 ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga
    61201 gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc
    61261 attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa
    61321 tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg
    61381 gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat
    61441 ggttggaatg atttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga
    61501 cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa
    61561 agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc
    61621 aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat
    61681 tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac
    61741 gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga
    61801 tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg
    61861 tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc
    61921 atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata
    61981 tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc
    62041 acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc
    62101 aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt
    62161 cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat
    62221 attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc
    62281 tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt
    62341 cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa
    62401 caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa
    62461 atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat
    62521 gaaccaacgg caggacttga tccccagggc agaaaagaaa tgatggagat gttttggcgt
    62581 cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc
    62641 aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg
    62701 caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc
    62761 accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca
    62821 gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa
    62881 attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc
    62941 taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac
    63001 gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa
    63061 attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca
    63121 ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga
    63181 atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc
    63241 gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct
    63301 tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc
    63361 tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc
    63421 tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt
    63481 gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga
    63541 agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa
    63601 tagagacacg atggttatca ttgcttttgt attattaact gtgctactga tctttttaag
    63661 aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag
    63721 gatgcaagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct
    63781 ctttctttta tctgaaaaga aagaacgaaa aaattttata gaaaaatcat tattccagat
    63841 aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcgtacg
    63901 acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag
    63961 tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa
    64021 ggacagatgc cggagtacat gcgatcgggc aagtaatcca ttttgattat ccagaggagc
    64081 gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa
    64141 gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt
    64201 atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt
    64261 attttcctta cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga
    64321 ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa atacgtacga
    64381 tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg
    64441 gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca
    64501 atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag
    64561 caggacctac agcacaccct gaaggacttt atttgtatga agtgaaatat gaataagagg
    64621 ttatgataga agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa
    64681 aattttgcaa aatttcggct tattttcgaa gaagctactt ctggaacacc gtttaagaaa
    64741 gaggtcgtga gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc
    64801 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    64861 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg
    64921 gacagtctga acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc
    64981 gtggttgttc ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt
    65041 accagacata aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa
    65101 atccttcagg caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca
    65161 agaatgcgtc tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc
    65221 ctttgtcata aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag
    65281 aagcttttct gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg
    65341 cttgataggc tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg
    65401 tcccataaaa acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat
    65461 agtattgacc agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg
    65521 cactagaact ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat
    65581 ttgcaggcac atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt
    65641 aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg
    65701 tatagaatgt aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac
    65761 tggtcaaatt atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg
    65821 ctagtggcaa tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc
    65881 ggaagccata tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa
    65941 acccatccat cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac
    66001 ggaaacctaa tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac
    66061 tgataccact gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca
    66121 agcggtaaaa cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat
    66181 aaggatcagt atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca
    66241 tactgttcgt attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat
    66301 gattgaatga aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga
    66361 aaaccactgt caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga
    66421 tgccccatgc caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac
    66481 catcgtcttg ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga
    66541 acagataggc ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat
    66601 tcaatggaac aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt
    66661 tatttttcaa cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca
    66721 gacctgcgta aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat
    66781 ttttcatgat tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca
    66841 gtgcctcgcc atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc
    66901 cgcctgccag taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt
    66961 ttgtcaatag acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat
    67021 aaaaactgga taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat
    67081 ccataacacg atgaatcccc aaaatcacta atggaagata aacaaatgca tccagccaca
    67141 tgatgatctc cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa
    67201 catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac
    67261 agccgacctt aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa
    67321 agaacaccag tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca
    67381 atcctagaga agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga
    67441 agctggcatg aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc
    67501 ttccaggata gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga
    67561 aactagctgc tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat
    67621 ttattttcca ccaaatttct atgaagttgt attcagttta tcatatcctt gtttttctct
    67681 gcttaaaaag agtctagttt tcacaaaaaa ataaaattta gaataaataa cttcataatt
    67741 tgttcgtata acaaaaataa acgcaaaaaa agggtaggaa tcaaaagtcc cacccttaaa
    67801 aatttttatt aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa
    67861 cgcgttcaac ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc
    67921 cgtcgaccat aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca
    67981 tagcgtgtga acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg
    68041 ccatggtaat ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag
    68101 actaacatat catatagaat gggaatttgc aagtacattt tcatagatac aattgacaat
    68161 tgagaagaat aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt
    68221 actttgggta actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa
    68281 tgggagtttt ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct
    68341 ggaaccattg aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat
    68401 gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt
    68461 ttgcgtaaag aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct
    68521 gtagatgttt atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc
    68581 caagaaaaag tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat
    68641 gtctttgtac aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag
    68701 cagtcgatct gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc
    68761 caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca
    68821 ttaaatgtat tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact
    68881 agtggagcta aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg
    68941 ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg
    69001 tttcgtggat tttctaagca aattccagat gttgtctcat taaatgcttc tgatcttgct
    69061 gcagccttca ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga
    69121 acgattttga cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact
    69181 gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag caaagcgtgc tttagctaaa
    69241 actccagact tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga
    69301 ctgctgtttg tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa
    69361 acatatgaac cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt
    69421 gtccaaggtc aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta
    69481 aaaatcggag aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat
    69541 ttagatggta taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac
    69601 gtgcatacag aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc
    69661 aaaatcaaag tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa
    69721 gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt atggtatcgt agcgatcgct
    69781 gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt
    69841 ggacaaacaa tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca
    69901 gataaagtga ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca
    69961 gaagtagcag atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca
    70021 gctatgcttt cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa
    70081 atgttgtcta gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac
    70141 ggtgtagaga tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct
    70201 aacccagata tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca
    70261 gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct
    70321 gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct
    70381 gtctatcctt atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag
    70441 tattcattca taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt
    70501 tttgttaaag gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg
    70561 caggagtcgg agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag
    70621 acttactgac ttattatcct tttcgctacg aagatattca ggaaagaaat ctaaacgaga
    70681 tccaggatca agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc
    70741 gatttggtta taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca
    70801 acgtttcttt ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg
    70861 ccgtatacgg taaatgggat gcaaagcgta aagccttgaa cgggatgaag attttaggtt
    70921 ctcaaacaat ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga
    70981 cattagttga tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc
    71041 tgccaaacaa ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta
    71101 tgcattttcc aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag
    71161 agttcttttt atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga
    71221 atggtttgga gatcctttat gatgtccaaa aattgaaaca atttactcaa aagctacctt
    71281 ttgaactgac aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc
    71341 cgcagcatat gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg
    71401 cgattgcgtt atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag
    71461 aaatcttggc acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa
    71521 ccatcgcatt gattacgggg tcaacgaaag caaaagaacg acgtgagctc cttgaaaaac
    71581 tagagcaagg ggaaattaac gtcgtgatcg gcacacatgc attgatccaa gatggagtcg
    71641 aatttcagaa tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc
    71701 ggaaagtact tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga
    71761 ttcctagaac attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac
    71821 ttccagctgg acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg
    71881 tactggattg gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc
    71941 tgattgaaga atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc
    72001 gtgaactatt tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg
    72061 aaaaagaagt aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta
    72121 ctgtaattga agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg
    72181 atcgatttgg attggcacag ctgcaccaac ttcgtggacg tgttggacgc ggaagtgatg
    72241 cttcctactg tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa
    72301 tcatgacaga gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc
    72361 ctggtgaagt atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg
    72421 tgacggatgg aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcaaataa
    72481 aggattggca gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc
    72541 aggataatcg ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag
    72601 gagtggaaac aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa
    72661 ttgtagaagg cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg
    72721 gaaaagaagc agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata
    72781 cagacgaaaa aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg
    72841 catcaatggt ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag
    72901 cagggaatac aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata
    72961 tcgaacgccc tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg
    73021 atatgctgga tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta
    73081 ttttaggttc tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct
    73141 taaacaacgg aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc
    73201 ttcagaatga aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg
    73261 gtgtggctga tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg
    73321 aaggaacagc aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca
    73381 aaggaaaaat gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg
    73441 attattccaa acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc
    73501 atggagcaac aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg
    73561 agacagatgt agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg
    73621 atatctgaag cgatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa
    73681 aattagccat ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact
    73741 cgcgaagaag tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc
    73801 gataaagtga cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt
    73861 atggaattcg ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct
    73921 gaacaaattg aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt
    73981 caaataaaag caaagaagga taaaaagctg catgcagctt tttatccttc tttttttaat
    74041 ctttttgaca cgtaaaagtc atttttctga taattctggc gataaacaga aattatgttg
    74101 caaagtaaag cggttttcta tataattatt aataattcat tacacaaaga taagaataac
    74161 ctttgttata aaacgattta ttctgtgtaa tgtccttttt tattaag
//
LOCUS       DACDKJ010000007        71162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDKJ010000007 DACDKJ010000000
VERSION     DACDKJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.007"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            161..391
                     /locus_tag="IV328_000538"
     CDS             161..391
                     /locus_tag="IV328_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8936707.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IV328_000539"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IV328_000539"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8936708.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IV328_000540"
     CDS             1802..1978
                     /locus_tag="IV328_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8936709.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IV328_000541"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IV328_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8936710.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IV328_000542"
     CDS             2593..3060
                     /locus_tag="IV328_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8936711.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IV328_000543"
     CDS             3145..4029
                     /locus_tag="IV328_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8936712.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IV328_000544"
     CDS             4146..4379
                     /locus_tag="IV328_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8936713.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IV328_000545"
     tRNA            4467..4547
                     /locus_tag="IV328_000545"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IV328_000546"
     tRNA            4554..4625
                     /locus_tag="IV328_000546"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IV328_000547"
     CDS             5140..5847
                     /locus_tag="IV328_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8936714.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IV328_000548"
     CDS             complement(5907..6551)
                     /locus_tag="IV328_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8936715.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IV328_000549"
     CDS             6678..6965
                     /locus_tag="IV328_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936716.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IV328_000550"
     CDS             7073..7393
                     /locus_tag="IV328_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8936717.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="IV328_000551"
     CDS             7474..8787
                     /locus_tag="IV328_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8936718.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="IV328_000552"
     CDS             8784..9503
                     /locus_tag="IV328_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8936719.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="IV328_000553"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="IV328_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8936720.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="IV328_000554"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="IV328_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8936721.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="IV328_000555"
     CDS             join(13064..13126,13128..14165)
                     /gene="prfB"
                     /locus_tag="IV328_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8936722.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="IV328_000556"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="IV328_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8936723.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="IV328_000557"
     CDS             14940..15833
                     /locus_tag="IV328_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8936724.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="IV328_000558"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="IV328_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8936725.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="IV328_000559"
     CDS             17770..18627
                     /locus_tag="IV328_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8936726.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="IV328_000560"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="IV328_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8936727.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="IV328_000561"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="IV328_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8936728.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="IV328_000562"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="IV328_000562"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8936729.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="IV328_000563"
     CDS             21383..22141
                     /locus_tag="IV328_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8936730.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="IV328_000564"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="IV328_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8936731.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="IV328_000565"
     CDS             23069..24100
                     /locus_tag="IV328_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8936732.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="IV328_000566"
     CDS             24097..24864
                     /locus_tag="IV328_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8936733.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="IV328_000567"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="IV328_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8936734.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26832..27149
                     /locus_tag="IV328_000568"
     CDS             26832..27149
                     /locus_tag="IV328_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8936735.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27149..27499
                     /locus_tag="IV328_000569"
     CDS             27149..27499
                     /locus_tag="IV328_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8936736.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="IV328_000570"
     CDS             27666..28604
                     /locus_tag="IV328_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8936737.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="IV328_000571"
     CDS             28619..29449
                     /locus_tag="IV328_000571"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8936738.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="IV328_000572"
     CDS             29493..30518
                     /locus_tag="IV328_000572"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8936739.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="IV328_000573"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="IV328_000573"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8936740.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="IV328_000574"
     CDS             31596..32036
                     /locus_tag="IV328_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8936741.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="IV328_000575"
     CDS             32039..32641
                     /locus_tag="IV328_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8936742.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32700..33806)
                     /locus_tag="IV328_000576"
     CDS             complement(32700..33806)
                     /locus_tag="IV328_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8936743.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34016..35017
                     /gene="ccpA"
                     /locus_tag="IV328_000577"
     CDS             34016..35017
                     /gene="ccpA"
                     /locus_tag="IV328_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8936744.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35354..37699)
                     /locus_tag="IV328_000578"
     CDS             complement(35354..37699)
                     /locus_tag="IV328_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8936745.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37978..38880
                     /locus_tag="IV328_000579"
     CDS             37978..38880
                     /locus_tag="IV328_000579"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8936746.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39093..40868
                     /locus_tag="IV328_000580"
     CDS             39093..40868
                     /locus_tag="IV328_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8936747.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40934..42520)
                     /locus_tag="IV328_000581"
     CDS             complement(40934..42520)
                     /locus_tag="IV328_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8936748.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42771..42995
                     /locus_tag="IV328_000582"
     CDS             42771..42995
                     /locus_tag="IV328_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8936749.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43137..44888
                     /locus_tag="IV328_000583"
     CDS             43137..44888
                     /locus_tag="IV328_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8936750.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44888..46672
                     /locus_tag="IV328_000584"
     CDS             44888..46672
                     /locus_tag="IV328_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936751.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46998..47849
                     /locus_tag="IV328_000585"
     CDS             46998..47849
                     /locus_tag="IV328_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8936752.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47903..49654
                     /locus_tag="IV328_000586"
     CDS             47903..49654
                     /locus_tag="IV328_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8936753.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49658..49828
                     /locus_tag="IV328_000587"
     CDS             49658..49828
                     /locus_tag="IV328_000587"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936754.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49868..50770)
                     /locus_tag="IV328_000588"
     CDS             complement(49868..50770)
                     /locus_tag="IV328_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8936755.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51001..52404
                     /locus_tag="IV328_000589"
     CDS             51001..52404
                     /locus_tag="IV328_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8936756.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52404..53837
                     /gene="ascB"
                     /locus_tag="IV328_000590"
     CDS             52404..53837
                     /gene="ascB"
                     /locus_tag="IV328_000590"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8936757.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53982..54908
                     /gene="trxB"
                     /locus_tag="IV328_000591"
     CDS             53982..54908
                     /gene="trxB"
                     /locus_tag="IV328_000591"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8936758.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55122..56849
                     /locus_tag="IV328_000592"
     CDS             55122..56849
                     /locus_tag="IV328_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8936759.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56921..57214
                     /locus_tag="IV328_000593"
     CDS             56921..57214
                     /locus_tag="IV328_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8936760.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57306..58166)
                     /locus_tag="IV328_000594"
     CDS             complement(57306..58166)
                     /locus_tag="IV328_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8936761.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58258..58686
                     /locus_tag="IV328_000595"
     CDS             58258..58686
                     /locus_tag="IV328_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8936762.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58848..59024
                     /locus_tag="IV328_000596"
     CDS             58848..59024
                     /locus_tag="IV328_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8936763.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59051..59497
                     /locus_tag="IV328_000597"
     CDS             59051..59497
                     /locus_tag="IV328_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8936764.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="IV328_000598"
     CDS             complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="IV328_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8936765.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61736..62707
                     /locus_tag="IV328_000599"
     CDS             61736..62707
                     /locus_tag="IV328_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8936766.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62731..64923
                     /locus_tag="IV328_000600"
     CDS             62731..64923
                     /locus_tag="IV328_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8936767.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64940..65416
                     /gene="ybeY"
                     /locus_tag="IV328_000601"
     CDS             64940..65416
                     /gene="ybeY"
                     /locus_tag="IV328_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8936768.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65394..65795
                     /locus_tag="IV328_000602"
     CDS             65394..65795
                     /locus_tag="IV328_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8936769.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65810..66709
                     /locus_tag="IV328_000603"
     CDS             65810..66709
                     /locus_tag="IV328_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8936770.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66852..67664
                     /gene="recO"
                     /locus_tag="IV328_000604"
     CDS             66852..67664
                     /gene="recO"
                     /locus_tag="IV328_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8936771.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68048..68965
                     /gene="glyQ"
                     /locus_tag="IV328_000605"
     CDS             68048..68965
                     /gene="glyQ"
                     /locus_tag="IV328_000605"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8936772.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68967..71042
                     /locus_tag="IV328_000606"
     CDS             68967..71042
                     /locus_tag="IV328_000606"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8936773.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23291 a        12456 c        15224 g        20191 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtt
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgc gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga
    31021 tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc
    31201 cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag
    31681 tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa
    31741 tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta
    31801 atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga
    31921 aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc
    32101 cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc
    32161 ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga
    32221 gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc
    32281 aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga
    32341 tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc
    32401 agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga
    32461 tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga
    32581 tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta
    32641 aaatcacaaa tttatagaat aaaaaggtgg gagcggacta gctctcacct tttttactct
    32701 tatttttcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca gccatcgctt
    32761 gtcacatata cacagtcttc gatacgtacc cctacttttt caggaatata gattcctggt
    32821 tcaagagaga agcacatccc ttcttcgatt actaagtcat ttccttctac aagggaaggg
    32881 aattcgtgga cggttgttcc gatcccgtga cctaaacggt gattgaaata ttcgccgtag
    32941 ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga
    33001 cggacggctt cttgtgcggc aagctgtgct tcaagagtga tgttgtagat tttttcttgg
    33061 aagtcggttg gtttttgata agcaattgta cgagtagcat cactgcaata gccattccaa
    33121 acaacgccaa gatcgaataa cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta
    33181 ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa
    33241 acaccttgtt ttttcaattg gtattcaatt tctgcaatga tcgcttgttc agtcacgcct
    33301 tctttcactg ctttaaagcc aatctcaaat gcaacatcgg cccatttacc agcttcgatc
    33361 agtttgtctt tttcctctag agttttgatc agttgtagac gttggatgat ggaagttaga
    33421 tcagtgtaag aatccgttcc cgtcaagata tcagagatag catcgaagcg ctcgacagtc
    33481 aaggcgttct tttcaatccc tacattgcca ggctgaccat agcgagcacg aagttctttt
    33541 ccgatgatct cccaagggtt ttcgctgtct aaatagccac ggacatcata cgcccaacta
    33601 ctgttttgag catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga
    33661 gggataaaaa gagctagtac acgctcatga ggatcacttt cgtatccgga aaagtaagcg
    33721 atgtggccag ggtcgctgat ataagccaat tcgacttggt gttgtgccat ccattgacgt
    33781 agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc
    33841 aataattgta ccactgattt attaaaagac caacgttcta aaaactgaaa aaagtaaaaa
    33901 ttttcatgaa aacagagata aaacggttgc aattcttgag attatttgat attctatttt
    33961 agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga
    34021 aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt
    34081 ttcacgtgtc gtaaatggca atccaaacgt taaaccagca acaagaaaaa aagtcttgga
    34141 agttatcgat cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa
    34201 aacaacaaca gttggtgtaa tcatccctga tgtcagcaat atgttctttg cttctttagc
    34261 acgtggtatc gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga
    34321 tggcaacgac caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg
    34381 cgtgatcttc atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa
    34441 aactcctgtt gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat
    34501 tgattatgta tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa
    34561 aatcgctttt gtcagtggtg cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg
    34621 ctataaagaa gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc
    34681 acaatacaac ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac
    34741 agctgtctat gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg
    34801 tggcgtgaaa gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga
    34861 agtagctcgt cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc
    34921 tatgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt
    34981 gccttatgga atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt
    35041 tgtgtgcctg ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg
    35101 tttgatgaat attgtgactc gtgattcgga gttggcagga gaacaccgtt tatttgggaa
    35161 aaggggttat gacaaaattt tgttataatc ctttttttgt gtcttaaaaa agagagaaag
    35221 tggttgaaat tacatttatc gtatataaaa caatccgtgt atagatgctg cattttgttc
    35281 accaccatca aaaaaataag ggactgtgac aatactattg tcacaatccc ttattactgt
    35341 gtattctatt aaatcaatca tctttctcat ctgattcttt agttgcttga ttcgctcttg
    35401 cataagctga agcggtgttc cagtaatctg tatagttttc atcggtaccg ccaacgccaa
    35461 atttaaaggc gcttttcttc ggtccatttt tcgcccatag cgattcaact gtttttcctg
    35521 gtgtttggat ggatttttta ttgacagtaa cttttccagg tgtttgacca gtaaatccag
    35581 atactttttt cttcatcaca tcagatgata attcgaattt ctcatcagtg ccaaaaatgt
    35641 caggttttgc ttggtaagct tgattgatca gattggcaag ataattagcc gaacgtatcc
    35701 ttgctttgct gtccattcca gtattgtcat cgtgccctgc ccagctactg atggtaatag
    35761 acggagtaga gacaattagc catgaatctc ggtattcatt ggtagaacca gtttttccga
    35821 cccaatctgc ttttcctaga tttccgttta agcttgagat ggcatctttg aatggtgtag
    35881 tgatttttgc attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttccg
    35941 aatagatccg gacagagttg ctttcgtgtt tataaattac atttcctgcg ttatctgtga
    36001 tggaatcgat gatatagcct tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt
    36061 tggtctgttg aagcgtcgtg acatctactg tacctaaagg agcagattca acgccccaat
    36121 tgtcatttgc cgggtagttc atctttgcca agtagtgctc gtaagcatat tgtttcgatc
    36181 caccggaatt caacacatct tgatataagt gataagccgg gatattgttg gaccattcta
    36241 gtgactcacg gacggtttgg aaggtgttcg atccttcgtt agtagcattg acgattttct
    36301 cgccagcatt ttctccttct tgccaagttg ttgcataatc tgatacacgg gattctgatc
    36361 cgataagccc catgtcgatt gcaggtccat aaacaagaac tggtttgatt gatgagccag
    36421 cttgacgttc tgtatcgaag gcatggttat tttgattctg actgtaattc cgtccgccaa
    36481 cgaatccgta gatgcggcca gttctgttat ccatcaatac atttccggtt tctacttgac
    36541 tgccagcacc atcatctaac atgtatccat aattttcaac gccattttgc aatgcagcat
    36601 agacatcttt atcgattgtc gaatggattg tatatccttt attttggatc gtttgctgtg
    36661 ccttttgata ataagcgtca ctgaccgatg aatcagacat atcagcgttg tctttttcag
    36721 ccaattgttg tgcaatgatt cttgttgctt cattcatcac agtataatac aagaattctc
    36781 tgtcagtctg ttctgcgatt tgttgtggta gaaagtcttt tgtaaggtca taggcttttg
    36841 cttcttcgta ttgttctttc gtaatttggc cttcacgata catactgaat aaaacaaaat
    36901 cttttctggc aaggccggca gaaagatctt cttttagcgc gcctgtgttc gtgtatggac
    36961 tatacgtgat cgggctttgg ggtaatccag cgatataagc agcttgcggc agcgtcacat
    37021 cttttgcatc tacaccaaag attccttttg cagcttcctg gactccagca atgttttgtc
    37081 ccttattatt acgaccaaat ggtgagacat tcaaatacgt agcaatgatc tcatcttttg
    37141 aaaagtattt ttctacttgt gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg
    37201 tttcatctgt aaggatctgc tgtttgacta attgctgtgt caaagttgat ccaccagaag
    37261 agccaatacc agtagcttca gatactaatg ctcgtaaaac agctttagga acgtaacctt
    37321 ggtgtttatc gaagtattca tcctctgtgc tgataattgc agttttcaat aaaggtgaaa
    37381 tcttatcaga tgaaatagtc gtgcgcatca gatctgtctg gatttttgat atctctgtat
    37441 tatctgcata aacaatcttt gacgtttctg taatgtttcc tagttctgtc tgcagtgctt
    37501 ttttagtcgg caattgtgtg tcttcgacga gataggcaaa atagccagcg cctattccaa
    37561 tacctagagc tgcgaacatc aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa
    37621 ctctaaaaat aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc
    37681 gagtattttt gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag
    37741 caaataatag aacaaaaaaa cagacaaggc tgtttttggc aggaatttct tttttaaaag
    37801 agttaaaacg tgagaaaatg atctttatta taaaagagtt cttaagcttt tctttagaga
    37861 aaatttcagt tactatggag aacagggcaa agaaaaatca atcattttct ttgtatatca
    37921 tcttaccaat atataggaat tgttagtaac aattgccttt caaggaggaa tccatccatg
    37981 ttattaggct cacacgtcag catgagcgga aagaaaatgc tgttaggagc agcagaagaa
    38041 gcagccagtt atggatcttc gacttttatg atctatacag gcgcccctca aaacactaga
    38101 agaaaagcca tcgaagagat gaatattgat gccggaaaag cgtacatggc agaaaaaggt
    38161 ctaacaaata tcgtcgttca tgccccttat atcattaatt tggggaatac gatcaaacca
    38221 gaaatgtttc cttttgccgt ccaatttctg agagaagaga ttgttcgtgc ggaagcttta
    38281 ggcgctactc aaattacact tcacccaggt gctcatgtag gggcaggaac agaagccgga
    38341 attgctagaa ttattgaagg cttgaatgaa gttttgacaa aagaacagac ggcacaaatc
    38401 gctttggaga caatggcagg taaaggtacg gaaatcggtc gaacatttga ggaattagcg
    38461 caaatcatcg atggagtaaa attgaatgaa aaattatccg ttacttttga cacctgtcat
    38521 acgaatgatg ccggttataa tattaaagaa gacttttcag gtgtaatgga agagtttgat
    38581 aagatcatcg ggttagatcg gcttaaagta ctccatatca atgattctaa aaatccgcaa
    38641 ggttcgcata aagaccggca cgccaatatt ggttttggaa caatcggatt cgacacgctg
    38701 aataagatcg tccatgatcc aatgtttgac aatatttcga aaatcttgga aactccctat
    38761 gtaggcaaag ataaaaagaa agcttacgca ccatatggaa aagaaatcgc catgttcaaa
    38821 gcgcaaacat tcgatccaga agtgtttcct gagttattag cagaagaagt gatactctaa
    38881 gattttctat ctgacagcag caaaaaagga ccaggcacat tgccttgtcc ttttttgttt
    38941 aagaaacaaa ggagtacttt ttgcagtctt tttcatcatg catcttctcc cccctctttt
    39001 taaatttaga caaacaaggt atactggtag aaaagtaaaa aaacgaaaag tttcacaagc
    39061 aagtggcaaa acgtgattgg aaggacgaaa agatgctgaa cactttaaaa cccttgatca
    39121 accaactaaa aaagaaacat acatacttta ttcttggcat agccctatta tttacaatta
    39181 ttcgtttgta tttatatagt aaagcaatct atggaacaga catgtcggca acctatgatg
    39241 accagctgct gattcgctat gctcaaaata ttttggacgg taaatggcta ggggagtata
    39301 cgactacaac actttccaaa ggaatctcct acagcttatt tttagtactt gcacataaac
    39361 tgcatctttc ttatgcagtt ttgttcggca tgttcagtat ttttgccagt atattggttg
    39421 cactcgcgtt taaaccgatt gtgaagaaca agtatcttct tttaagtatc tatttttatt
    39481 ttttgttttc tccgattagt tttacacatg aatattccac tcgaacatat cgtaatgccc
    39541 tcgtgattcc ggctgttgag atcattatcg cttgtttatt agctatttat taccgcaaat
    39601 acggttctag cagaaaattg ctgccatggt ttttagggct ttctgtcgct tttcctttct
    39661 tttggttcat tcgcgaagat tctatctggc ttcttccttt tactgtagta gctttgggta
    39721 tcagtttcgt ccagatcttg ctagggcaag acttttcttt caaagaagta aagaagttaa
    39781 aaaaacgact ctttcttctc tcgaaacggg aatggctgaa actttcattg tttttacttc
    39841 ctatagttgg cttttccatc gtctatcaat ggatcgaata taaaaatgag acaacatacg
    39901 gtattgcaac catcaatgac cgttccagtg gagaatttgg acgtttgatg aaacaactga
    39961 tccgaataga tgatgaaacg aacttgaata aagaaaacag tgagatatgg gtctcacatg
    40021 ctgcactaaa taaagcatta gccgcatctc ctacattcca tactgtttct acccgtattg
    40081 acgaattata tacgactcac gcgtggactc aaggtggaaa agtaaaagaa ttacccggag
    40141 atatcatctt ttgggcattg cgagatgtgg taagcgaatc tggttattac caaaacaatg
    40201 caaaagaaac caatgatttt tggaaaaaag tcaaccaaga attagaagaa gcctatcaca
    40261 agggaacaat caaaaagaaa aaagagttct atctgatggg atcaggagat ggaaagacga
    40321 tgagtgatct gccaatcgta ttcgatttcc ttcaatcagg atacgcttat gcatggcgat
    40381 ataaagattt ccatcaagga ggaaattata gtttcggaac gcaagaacaa gtcaaacagg
    40441 ctcaagaatt attaaacctt ccgctaatgg agaattggat cgatagtaat gaaccgagaa
    40501 cacctaattt ccaattaaca aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc
    40561 aagttctggc tattcccgtg ctgattgctt caatagcagg gatattactt atgatactag
    40621 gaaccgtatt tgcaaaagct gatcgtgcct attatagttc ctctttgatt gtggtcaccg
    40681 gattaatact gacacaattg gtttttcttt tcggtgtctc atggttttgt tcttatgcgc
    40741 cagaaaaaaa ggactatttt ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat
    40801 tcatcgtagt aacaagcatg ctagttttca gtaaattaaa tctgaaaaaa gctttatctt
    40861 ctaaataaaa agacccttgg tagctagatt tttaacctaa ctatcaaggg tctttgtgtg
    40921 gaaaaatcag tttttattcg atttcttcta ccgtttcctg caaaagctgt tcatattttt
    40981 tgtttccttt atataaatca ccaatcgtcc atgccaacag gagaaggaca agaacaatga
    41041 ttacataagc gatgtgatca gccaatgcta gttgagcgga agtggcttgc tcgccgaaga
    41101 aactttcgat ctgtcctggt aatcctcgta agttcaagta agttagacta atcacagaga
    41161 accagccaag aagttttacg atgaatgaat ttttaaatcg gctacccatc tccgcttggc
    41221 tgttggtcat catcagtaaa ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct
    41281 gggattcatt cattaagtta ttcaaagcgg catgttcttc gatcgttcct tttgtacttg
    41341 taaacaaaac acaaatcaat acaggaatca cagaaagcaa tcgtgtgacc agtcttctta
    41401 gccaaatcgg gatacgcata tggatgaatc cttccatgat gacttgaccg gtcaacgtac
    41461 cagtaatagt agagttctgg ccagaagcaa gaagtgctac agcaaataaa gtggacaaga
    41521 taccagattt tgctacagca atcaaaatac cattgctcat agctgatgga tcagataaag
    41581 cttcatacag accaaaaaag gaaggatctt ggacagaacc ggttttgaag acagcgacac
    41641 ccatgatcaa tagcagtgca ttgactagaa aagccattga caactggata ttagaatccc
    41701 aagtagaaaa acgtacagct tgtgctacat cttcttcgtt ttggtgatcg atcttccgag
    41761 tttgtgaaac tgctgaatga agatataagt tatgcggcat gactgttgca ccaataatcc
    41821 ctaaagctcc agtcagcgga gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac
    41881 tgttagggat aaagccagca aataaactgt gccaatctgg gttagacaag ccaacttgat
    41941 agacaaaaac gaataaaatg acgaaaatta agcagacaac cagtgcttcg atcttacgaa
    42001 acccgacttt tgtcagtagt aagagaacga aaacatctaa tacagtaata aagacggcaa
    42061 tcaccaatgg aatatcaaaa agtaagtaga gggcgattgc tgcaccaatg acttctgcaa
    42121 tatctgttgc cataatggct aattcagtca agatccataa aatgacacct aatgtcttac
    42181 ttgtgcgtgc tcggattgcc tgtgccaaat ccatttggct cacgattcct aattttgctg
    42241 ccatatactg taaaagcata gcaatcagac tagacatcaa aataactgac atcaataaat
    42301 attggaaatt ttgtcctccg gtgatcgaag tagaccagtt acccgggtcc atatacccaa
    42361 ctgctactaa cgcaccaggt ccagagtaag caagaagtgt tttaaaaaaa cttttcccct
    42421 ttggtacatc aactgttcca tttacttcag ctaaagataa accatttgta tgttcaataa
    42481 gtcgatgctt atgaaattgt ttattggatt gattattcaa gaaaaaacca cctttcctat
    42541 ttatctacga ttacattcta cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg
    42601 cctaattttt tttcatggcg cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa
    42661 aaaaaccact attttttgca tgaaactaaa gaatacgcct ttcttttttc aataaacttt
    42721 agtaaaataa gtcattgaga gcaattcgga aatagaaagg agagatttat atgttatcaa
    42781 caggtattgt cgtacttatt gctattattg ctttgctaat cggcgcagta ggtggctttt
    42841 tcctagcgcg taaatatatg aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca
    42901 tgttgcgatc aatgatgatg tcaatggggc aaaaaccatc tgagaagaaa attcgccaga
    42961 tgatgcagca aatgaagaac caagggaaaa aataggttct gacccaacga ctttttacgg
    43021 gctgattttt tcagtccgtt ttttatttcg taatttgtta gactattcag attattcagc
    43081 aaaaaagaca agcacagaca atcctaggct cttgataaag acaaaggagg agtaaaatgt
    43141 cgatttttag aaaattaggc tggttttttc gtcaggaaaa gaaacattat ttaattgggg
    43201 ttttctcgct attcgcagtg gccttggtac agttagttcc tccaaaagtg attgggatca
    43261 tcattgatga gatcgccgat aaaaatttat caataaagat cattttcggg tggatcttcc
    43321 tattgattat cgctgctttt ttacaatatc tttttcgtta tatctggcgg acgaatattt
    43381 ggggaagtgc agctcgattg gagaaagaac ttcggcagca gttattcaaa cattttacag
    43441 aaatggacaa tctcttttac caaaaatatc ggactgggga tttgatggct cacgcaacga
    43501 atgatttgaa tgcgatccag aatgtagcag gagctggtat cttgaccttt gcagattcag
    43561 tgattacggg cggaacaacg atcattgcga tgattttgtt tattgattgg cgcttaacac
    43621 tgattgcgct gattcctctg cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac
    43681 tccatgatgc ttttcgtgat tcgcaagaag ctttttctag tatcaatgat aagacacaag
    43741 aaagtatcac tgggatcaaa gtcattaaaa catttggaca agaaaaagaa gatctagcgg
    43801 attttgaaga aaagattgaa gatgcaatta ttaaaaacaa acgagtcaat tttttagact
    43861 ccttatttga tccattcatt accttgatta tcggtctgtc ttatgttgca acaattattt
    43921 taggtggaaa gtatgtcttg gaaggcacga tctcgatcgg gcagatggtc tcatttatta
    43981 gttatatcgg tatgcttgtt tggccgatgt ttgcgattgg tcgattgttc aacgtattgg
    44041 aaagaggaaa tgcttcttat gatcgcgtca atgaactttt gcatgaaaag acgcatatcg
    44101 tggaacaact gggtgcagtg cagaccccgg caaaaggcga gctgaatgta gctatttcct
    44161 cttttaatta tccgaaagaa gaatcaccag cattagaaca aatcaagttt gcgatccaag
    44221 aaggagaaac attaggtatt gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat
    44281 tactgatgcg tgaatacgat gattatcaag ggaaaatcca atttggcgga cgttcgatca
    44341 aagaatattc attgaatgct ttattaggat caatcggcta tgtgcctcag gatcacttct
    44401 tgttttcgat gaccattcga gataatatcc gtttttctaa tccaaaactt tcagaagaag
    44461 aagtagagca tgcggcttct ttagcattta tcaacaatga aatcaaggaa ttaccaaaag
    44521 gttatgatac cttagttgga gaacgtggag tctctttatc tggcggacag aaacaaagga
    44581 tttcgattgc tcgagcattg atcaaaaatc ctgaattatt aatattagat gatgcactct
    44641 ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa tttaaaagaa ataagaaaag
    44701 agaaaacaac tatcattgca gctcaccgtt taagtagcgt gatgcatgca aaagaaatca
    44761 ttgttgtcga tcaagggaaa atcattgaac gaggaaccca tcatgagctg cttgcactag
    44821 gcggctggta tgaacaaatg tgggagaaac agcaattaga agcgaagata gaaggaggcg
    44881 aggcctgatg gatgaaaatt atcaatcgga atggacaaaa tcgattccac ttaaagaaca
    44941 gaaaaagatc atcaaacgac tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt
    45001 tgctatctta tttgcgtttg ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca
    45061 aacttttatg gatgatcatc tggctaaaca gtcggctacg acacaagtta ttttgttttt
    45121 tgctggtatg tacttatttg gagtcatagt aaaaagtatc atttggtttt tccaatggta
    45181 tttgtattcc atggcctcat tgaagaccta tcagtatatt agggtaaaat tatttgaaaa
    45241 gctgcataca ctaggtatgc gttattttga tcagacaccg gcaggatcaa ccgtttctcg
    45301 agtgacgaat gacacagaaa cgctatttga attttggtac gtctttttga tggtattgac
    45361 tgggatcttt gcagtcgttt cttcattcct tgccatgttt caaatcaatg gagaaattgc
    45421 cttgtggact ttggtgtttt tgccgatttt aggaatcgtc atctggtatt accagaaatt
    45481 cagttctcgt atttaccgaa atatgcggga aaaattaagt cagctgaaca cgaaattgaa
    45541 tgaatcgatt tccggcatgc gtattatcca gcagtttcgc caagaagctc gtttagctaa
    45601 agaatttgaa caagtgaaca atgagtattt agacacgcgt tatgcgatga tcaaaacgaa
    45661 ttcgattctg ttaagtccga tcatcaattt tctatatgca ttggcgattg cgttggtgtt
    45721 gactttgttt gggatcgatg cgttgcattc tcccgtagaa gttggactga tctatgcatt
    45781 tacgacctat gtccagggat tttttaatcc aatgtctcaa atgatggact ttttgagcat
    45841 cttcacagat gggatggttg ctggtagtcg tatcttgaag attatggata cggaagaata
    45901 tgcaccacaa caaaaccctg gagcagatca aatgattact tcaggtaaga ttgagtttcg
    45961 caatgtaagc ttttcttatg atggagagca caatgtatta aacaatatct cttttgttgc
    46021 taatccggga gagaccgttg cattagtggg acacaccgga agcggaaaaa gctcgatcat
    46081 caacgtattg atgcggtttt acgaatttta tgaaggtcag atattgattg atgatcatga
    46141 cattcgagaa tatccaatgg aagagttacg taagaaaatg gggctggtct tgcaagatgc
    46201 tttcttgttt tatggggata tcgcaggaaa tattcggatg atgaatccag ctatcacaga
    46261 cgaacagata cgccatgcgg ctgagtttgt tcaagcaaac aaattcatcg atgaattacc
    46321 aaaaggatat cactcaaaag tgatcgaaca tggtgccagc tattctagtg gacaacgaca
    46381 gctgatcact tttgcgcgaa caatcgtgac tgatcctaaa attctagtat tggatgaagc
    46441 aacagcaaat atcgatactg aaacagaagg attgatccaa gaaggattgg caaaaatgcg
    46501 tcaaggacgg acaacgattg cgattgccca tcggctgtca acgattcgag atgcagaatt
    46561 gattttagtt ttagacaaag gtcatatagt agaacgcggg aatcatgaaa cacttctagc
    46621 gcaaaatggt ttgtatgcag atatgtacaa actgcaaagc agtggtatat aaaagatgtt
    46681 gtgacagtag aattttgacc taagcactag agtgaaaata aaacatagct tctcatattt
    46741 ttcatctgtg agagttagtg tcgtagtcac cagatttaaa aaaagattcg acagctagtc
    46801 agaagtgcct aatagccact aagaaagcgt tgacaaaagc atttgctttc gataatataa
    46861 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    46921 atggtttcat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatacatc
    46981 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    47041 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    47101 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    47161 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    47221 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    47281 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    47341 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    47401 aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat
    47461 ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccagcaagac aatacctacc
    47521 tgtttactat aacaatacag gatattctaa atatcgtccg ctattactat aagattacat
    47581 ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cttacgtttt tttctgacac
    47641 gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg tttcaggtta
    47701 ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg
    47761 agacgttcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc catactgcgc
    47821 gtttagtgca tgagaatcgt tgtaaataaa aaaaggagga taaattaaca agaacgtatg
    47881 aaataagggt agtgacattc agttggctaa accttccact aataaattgg agggaataga
    47941 aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa
    48001 tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa
    48061 agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc gagcgggagg
    48121 gcagtatcaa gttgtcattg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc
    48181 aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct
    48241 tattgatgga atatcgggct gtttccagcc attccttgct atcctagctg ctggcgggat
    48301 gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg gttctggcac
    48361 atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg
    48421 tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc
    48481 attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactaac
    48541 gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc
    48601 ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc
    48661 tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt
    48721 tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg gaccagtatt
    48781 cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt
    48841 tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg gtctccattg
    48901 ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atattatgac
    48961 acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct actttaagct
    49021 tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta ttgtggggat
    49081 tacagagcca gctatttatg ggtttacatt accacgtaaa aaaatattta tttttacctg
    49141 tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt cttggaatca
    49201 aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacaga
    49261 tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatccttta cgctgacttt
    49321 tttcttttgg aaggatgaag caggtgaaac agatgatata cagaaaaaat caggtaaaga
    49381 aattgtaaaa acaccgatac aaggacaaat cgctccacta aatgcagcaa aggatgcagc
    49441 ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg
    49501 tgcccctttt gacggaacag tcatgaccct gttcccgacg aaacaggcaa ttggaatggt
    49561 ttctgaaaca gggctggaat tgctgattca tgtcggattg gatactgtgc agttggaagg
    49621 gaaatatttt gaaagcttag tgcagcaagg ataaagagtg aaacaaggcg atctgttgct
    49681 acgcttcgac aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt
    49741 caccaattac acggattatt tggatatttt ggaaaaccaa ggggagacgg tcgggccaga
    49801 cgatgcactt atcaccgcac tgacctgaac ggttaggtga agaatggact aggcttaact
    49861 attcttttta ttccacttct ttattttcaa aattgaaagg ctctttcatg atggctgagc
    49921 tgatagggcg tttatcgaat ttttgaccga cagaaattag atggactaaa ttctgttgat
    49981 aattttctgc aaaaacacaa ctatataaca cgtctagaag ataacaaatt gatgtattga
    50041 tcgtaaaatt accaattttt gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg
    50101 tactcattgc agatagcgtt gaatcaccga tactggtcaa agctaaaatt ggaacatgat
    50161 tttgtttcaa tatttctgcg gtacgaagga tcacattgtt ttcacccgta taagaaatca
    50221 gaatcccgca catatcactc gtgcaattgt acgcttcata gaatgattca ccaaaagtgg
    50281 tgacaatcgt gacatccatt ttgatgcgct tcattttcag catgaaatct tgagaaatca
    50341 aagtattcga gttactggcg aaaatcacga ttttttttgc ttgaagcatt agttgtttgg
    50401 ctttttgcag ttggtcatga tgaatgagag aaagtgtatc atgaatcgtc atctgttcta
    50461 aagaagccat tttatttgcg atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg
    50521 catctacgtc ttgaaaattc ccttcaagat aggctgtttc agctaaaaaa gcattacgaa
    50581 aactgaccca gccatcaaaa cccatttttt ttgctacacg gatgagtgta gaggggtgga
    50641 cataacatcg ctcagcaatc gcttggatgg tcatttcgcc aagtgctgga gaatcgctta
    50701 agagaaactg gatcacttca ttttctgaag gagaaaagga gatttctttg attttttcac
    50761 gtaataacat gtagatacct tcttcttatt tttttacgta aattataact tttttgaaaa
    50821 tgatttgtaa acggatttat actatataac ttaaaataga cattttttga caagatattt
    50881 acaaaaatcc actagaaaac agtaaatttt gtaaatgtta ctggaaagaa acagatttct
    50941 attgtttgtt ttgtttacaa aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac
    51001 atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccccgagttg
    51061 atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat
    51121 gtagaagaag gaaaagaaaa gcttgagatc gttagtcaga tgtctcagcg tatgtgggat
    51181 gcttctccgt atgaagtcaa gatctatgca actctggatc gggaagaagc attgaaagat
    51241 gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag
    51301 cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc
    51361 aaagcttttc gtaccgttcc agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt
    51421 ccaaatgctt ggctgatcaa tttcgcgaac ccaagtggga tggtgacaga agcagttatc
    51481 agatatggca aatgggataa agtgattgga ttatgcaatg tgccagtgat ggcgatgatg
    51541 acagaaccag caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta
    51601 aatcatttcc attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg
    51661 attgatcgtt tatacgatga agagaatgga ttaccgaaaa atatctttga tatcccgttt
    51721 ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactat
    51781 cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg
    51841 cagcaagtga aagaaacaga agcagagcta tttgaattat acaaagatcc agaattagac
    51901 cataaacctg aacaattgca aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa
    51961 acgattgctt caatctatgg aaataaagaa acacagatcg tggtatctac aaagaatgat
    52021 ggagctgttc ctgacctgcc ggcagattgt gtagtagaag tcactgccta tgttggcgca
    52081 caaggagcga gaaatgtagc gtttggttct cttcctgctg ctgaaagagg ctggttgcaa
    52141 gtaatgaaga atatggaatt gctgacgatc gaagcagctg tgactggtga ttatgggaca
    52201 gctttacagg cgtttacgat caatccactt atacgatcag gaaagacagc aaaacggatc
    52261 atggatgagt tgtttattgc acaccagcag tatttaccgc aattcaagga gacaatcagc
    52321 cgattaaaag aagaaggaat cgaagttcaa gatgaactgg caagagaatt atcaggtaat
    52381 acacatataa tggaggaagt ataatgggat tcagaaaaga ttttttatgg ggcggtgcta
    52441 cagcagccaa tcaatgtgag ggcggttatg atgaaggtgg acgcggacta gcaaacgtag
    52501 atgttgtgcc aatcggaaaa gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg
    52561 aatttgatga ggatcatttt tatccagcaa aaattgcaat cgatatgtat catcgttata
    52621 aagaagatat tgctttgttt gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct
    52681 ggagtcgtat ttttccaatg ggggatgaga aagagccgaa tgaagctggc ttgaaatttt
    52741 atgaggatgc ctttaaagaa tgccaaaagc acggaattga gccacttgtg acgattacgc
    52801 attttgattg tccaatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg
    52861 ttggttttta tgaaaatctg tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat
    52921 attggctgac attcaatgag atcaatatga tccttcatgc accattcatg ggcgcagggc
    52981 tgtattttga aaaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt
    53041 tggttgcaag tgccatcgca acgaagattg ctcatgaagt agatccggag aacaaagtag
    53101 gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg
    53161 cttctagaaa atcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat
    53221 atcccgctta tgcattgaaa gaaatggagc gaaatggcat tcagatcgaa atggaaccag
    53281 gggatgccgc attgctgaaa gaacatactg ttgattttat ctctttttct tattatgctt
    53341 cccgagtttc gactaccgac aacgaactct tggaacagac tgcaggaaat atctttgctt
    53401 ctgtcaaaaa tccatatctg caagcaagtg aatgggggtg gcaaattgat ccattaggct
    53461 tgagaataac gatgaatgat atttatgatc gttatcaaaa accattgttt attgtagaaa
    53521 acggcttagg tgcagtcgat acgccagatg aaaatggtta tgtggaagat gattaccgaa
    53581 tcgattacct ggcagcgcat atccaggcga tgaaggatgc agtagaacta gacggagtcg
    53641 atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt accggtgaga
    53701 tgaagaaaag gtacggattc atttatgtcg atcgtgataa ccaaggaaat ggcacattag
    53761 cccgttcaaa gaaaaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa
    53821 atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg
    53881 agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct
    53941 gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta
    54001 ttggcgcagg tcctgctggg atgactgctg ctttatatgc ttcacgttca aacttatccg
    54061 tattgatgat cgaacaaggt gcccctggcg gccaaatgaa caatacggca gaaatcgaaa
    54121 attatcctgg attcgattcg atcatgggcc ccgatctagc ttataagatg tatgaaagtg
    54181 tctcaagatt tgggacagaa aatgcttatg gaatcgttca agatatcaag gatcacggca
    54241 gccataaaga agtcatctgt gaagataaaa gttacgaagg aaaaacagtc attatcgcaa
    54301 caggatgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgaggcg
    54361 tttcttactg tgcagtctgt gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg
    54421 ggggtgggga ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag
    54481 tgatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg
    54541 caaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa
    54601 tggtagtgac aggtgtgaaa gcacgtaatg tcaaaaccgg caatgaattt gatttagcag
    54661 cagacggagt attcatctat gttggactgg atccattaac tgcacctttt agagaagcag
    54721 gtttgacgaa cgaagctggc tggattccaa cagacaacga aatgaaaacg aatatgccag
    54781 gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact acagctgttg
    54841 gtgacggcgg tattgctgga caacaagtgt ataaatactt ggagtcttta gaagaaacaa
    54901 cagcataagt ttaaaaagag tagaagaacg ggttcaattg ttcttttact cttttttttc
    54961 gcgtgaaatt tactgaatct gatttatgcg aacacgtcca acaccttttg ataaaagaat
    55021 cctttcttga tttaagcgtt ttttggtcct catactttca tatctaataa gtgaatgcta
    55081 tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg gaacaagtgt
    55141 atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc
    55201 tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa ttcggtactg
    55261 caggtatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat acggtaagac
    55321 aagcaacaga aggactagca cgtttcatgg atacacaagg tgaagaaaca aaacgccgag
    55381 gtgtcgctat cgcttatgac tcacgccatc agtcaccaga atttgcaatg gaagcagcca
    55441 aaacactagc acatcacaat atcccgtctt atgtattcga aagtctgcgt ccaacaccag
    55501 agctttcttt tgcagttcgt catttgaaga cgtttgcggg aatcatggta accgcatctc
    55561 acaacccagc ggcttacaat ggatataaag tctacggaga agatggcgga caaatgcctc
    55621 ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg
    55681 aagtattatc tgacgaagaa gctaaacata gcgggttgat cacgatcatc ggagaagaag
    55741 tggacgctgc ttatctagaa gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa
    55801 caatggggaa agatttgaaa cttgtgtaca cgccacttca tggaacaggg aaaatgttgg
    55861 gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttccc gaacaagctg
    55921 tggcagatcc agatttcact acggtcaaat cgccaaatcc agaagaacat tcagcttttg
    55981 aatatgctat acgacttgga gaaaaagaag gagctgactt gctgattgca acagatcctg
    56041 atgcagaccg tttaggcgca gcggttcgtt tgccagatgg ttcttatgaa gtattgactg
    56101 ggaaccaaat cggagcattg tttgctcaat atattttgga agcacatcaa tcaagaggca
    56161 ctttaccaga aaacgcagcg gtattgaaat caatcgtttc cagcgaattg cctacagcaa
    56221 ttgcggaaca ctacggtgct accatgttca atgtgttgac agggtttaaa ttcattgctg
    56281 aaaaaatcca acaatttgaa gaagaccatt cacatacttt tatgtttgga ttcgaagaaa
    56341 gttatggtta tttagttaaa ccttttgttc gcgataaaga cgcgatccaa gcattgcttt
    56401 tattagcaga agtagcagct tactataaaa aacaaggcaa aacgttgtat gacggcttgc
    56461 aaacgatttt tgaaacctat ggctatttcg cagaaaaaac gatttctgtg acaatgagcg
    56521 gacaagaagg ttctgcgaaa atctctgctt tgatggaaaa attccgtaaa gaagcaccga
    56581 cagaatttgc cggagcaaaa gttattcaaa cagaagactt taaagaattg acgagaacag
    56641 accgtgaagg aaacgtagaa aaactaacaa caccgccatc cgacgtattg aagtatacat
    56701 tggaagaaaa tggctggatc gctatccgcc cttcaggaac tgagccgaaa atcaagtttt
    56761 atattggtgt aaaaggaagc tctacagaag acgcagccga aaaagtagcc tcttatgaag
    56821 cgattatcaa aaagatgaca gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa
    56881 ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc
    56941 aaaagtagag aaagtcagtc aattattcaa aatgttgagt gatcccactc gtcttaaaat
    57001 tctgctatat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtggaaat
    57061 ggagcagtct gctgtatcgc atcaactacg tttattaaga gaaaatcatg tcgtcaagtc
    57121 tcatcgcgag ggaaaagcga ttttgtatag tcttgatgat tctcatgtac tggatatttt
    57181 gaaccagaca ctcaaacatg tggaacatca ataacgatag gtgaaaatag ataaacactg
    57241 gagtgtgaca acgaagaaat ttcgttgtca cactccagtg ttttttaaga aaaagacaaa
    57301 aatgattaat cgcttttttc tgttgtttca gttccataat aactatgatc ttctaggtct
    57361 aaagcattca aaataagcga agccgtttct tcaatagata aagatgccac attgatcact
    57421 acacatccta gtttttcata aagatcattc gcaaaatcga gttcagctct aattttttcg
    57481 atatcggaat aagcagtatc agggtttaat ccgtaagagc gcatccgttc tttacggata
    57541 ttatttaaaa tatctgggtt attcgttagt cccacgattt ttttaggatc gatttcccac
    57601 agctgtttgg gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt taaattttta
    57661 tttgctaaaa acaagctcaa aggcgttttg gaagttcggg atacgcctag caaaacgaca
    57721 tctgcttcta aaaaaccacg aggatctttc ccatcatcgt attttacagc aaattccatt
    57781 gctttgatcc gtttgaaata gttctcattc aataaatgga gggcaccagg ttgccgaatt
    57841 ggcttgatac cagttaaacg ttcaatttct gatatcggtg gcgtcatcac atcgaaacta
    57901 aataattgat gttcttcaca aaaagaacgt gttatctcga tcagttcttg attgattaat
    57961 gtatgcagga caagtgcttt ttcttctaat gcatcttgca atgcctgcct taatgttttt
    58021 tcatctgtca cgaacgttct gcggaatagg ttgaactcaa cggtaggata ctgagccata
    58081 gtagcttgcg ctagttttgt cgcagtttct ccagcagagt cggagatgac aaaaaaagta
    58141 acgatttctg tagaatcgga agacatggat aaaacctcac ttgcattttt tttatgttta
    58201 ttataccata ctctagaatg cgtacttcgt aatcattttg atttggagga ataagtcatg
    58261 aaaaaacaga cattgatcga acaatcatta gaaacattga aaaataacgg ttttaaatat
    58321 actaaaaaaa gagaagcatt gttaacttat ttagtgaagc gtaaccgcta cgtatctgct
    58381 aaagaagttt ttgactatat gaatcaggaa ttcaaagggg taagttatga tacgatttac
    58441 cggaatctcc atgattttgc agagatcgat ttactggaag aaaccgattt aaatggtgaa
    58501 atgaagtttc gttttcattg ctgtcagggg gaaatcggcc atcaccatca ttttatctgt
    58561 acagtgtgcg gaacaacgaa agaaatccat atgtgtccaa tgaattattt cgaagaacag
    58621 ctaggtggat gcacgatcga agggcataga ttcgagattt tcggaagatg tgaaaattgt
    58681 caataaggat ttaaataaaa taacatttga taatttaaaa aaaaagcaag atttccttga
    58741 cggtcaaaac agcattagat ataatattta caggacagtc ttgttttttg agatatattt
    58801 caataacaat tacggttact ttttaagctc ggagggaggg aattaacatg tcaaaaacag
    58861 tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa
    58921 aagcgggtac tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac
    58981 gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg
    59041 agttggtagt gtgtcactac taagtagatt aaacgatgac atgaaagcag cgatgaaagc
    59101 gaaagataag gaatctttac aagtcatccg aatgatcaag tcatctatac aaaacgagca
    59161 gattaaagaa ggtcacgact tgacagaaga agaagaacta acggtgttgt ctcgcgaaat
    59221 gaaacagcga cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg atttagctga
    59281 aaaagtaaaa agtgaaattg taatagtcga aaaatatatg ccggaacaac tttccgacga
    59341 agaaattcga caacttgttc aagaagcaat cactcaaact ggtgcttcat caatgaaaga
    59401 atttggcaaa gttatgggtg caattatgcc taaagtcaaa ggaaaagcag atggaaacca
    59461 agtgaatgcg attgtgaaag aactattaca atcttaattt tatcatttaa actgcggaca
    59521 gtgattgtcc gcagtttttt gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag
    59581 aataagattc tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg
    59641 tttgatttgg ttattcttct ggtagttcat ttgcttcttg tttataccct gataggcgat
    59701 aggcgtaata aaagagtaag ctgccagcgt aaccaagtaa cgttgggacc atccagccaa
    59761 gtcctaatgc agcaaaagga acgtgatgat gataaaatcc tagtaagctt gctacgacag
    59821 tttgctgact cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag
    59881 aaaagaacat cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg
    59941 aaagcaagat taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt
    60001 ggatgatatt atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca
    60061 gccatgtcat ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa
    60121 cgagtcccat agcggtagtg aagacaccta atgtgaccat tacccctaag aagctagcac
    60181 cgaagttacc taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata
    60241 aaggcatctt attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt
    60301 ctaacgcaac gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa
    60361 aaccaagatc acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat
    60421 caaccgtgtt gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg
    60481 cagcagattg gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta
    60541 ataatgcaag aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat
    60601 gtacagtaag gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg
    60661 aatcatattt ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc
    60721 gcggtgtacc aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat
    60781 accattttcc gacaggttta gctaagtcat acagaccatc actttttgtc actactacag
    60841 ctaagatagc taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga
    60901 tccagtttga tccagccatt tgacctaagt ggacaggaaa gatcagatta cctgacccga
    60961 agaacatccc gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg
    61021 tttcttccat aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca
    61081 gaaactagaa atgccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa
    61141 aaagcccttt tccacttggg aaaagggctc tagattctag aacggtacca ccttttttcg
    61201 cttaaaaaag cttcttcatt cacggacgct tttcgataaa gttatccgct agcctgataa
    61261 tggaggcagc cacaatgatc tacgttttcg acattgactc ggaaatgatg ttcgtctatt
    61321 cgatttattg tcatttctca cttacatgac tcactgggaa taaatcgctg aatagcctac
    61381 tgttttcctc gacgctttgc tgtatattta tttgttgacg ttataataca cctgtttttt
    61441 ttgaaatgca agaatatttc aaatattttg ttttcttttt ttgaaacaag aaaaataccc
    61501 agagaatact tgctaagtta gacggaatac ttgtcctcta tcttatgcat agcttacatg
    61561 atcagctttt tttattcaaa cgagaccttt agagaatgga agcagctatt ttttaacgtt
    61621 tcttggtact tatggttaag caactctttt agaacctttt cttatttgct atatagtttc
    61681 ttctgagtat ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc tatttttgac
    61741 ggaagagtct agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt
    61801 aggtgcacac gacaagcata taaaactgat tgaagaaaca acagaaacaa ccatccatac
    61861 acgtggagaa atcattcaga tcattggcga acaatcccag atttccttgg ctgcctcagt
    61921 cattcggaca ttgcaggaat tgatcaaacg aggaatccat gtaagtacac cagatgtcgt
    61981 gacggcactg aaaatggggc aaaaaggcac attagattat tttgttgaaa tgtatgagga
    62041 agagatcgtc aaagaccgta atggaaaacc aatacgggta aaaaacagcg gccaaaagaa
    62101 atatgtcgaa tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg
    62161 aaaaactttt cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa
    62221 aatcattttg acgcggcctg ctgtggaagc aggggaaaat ctaggctttc ttcccgggga
    62281 tctaaaagaa aaagtagatc cttatctgcg tcctgtttat gatgctttat accaaatttt
    62341 tggattagat catacgaacc gattgatgga acgaggcgtg atcgaaattg ctcctttagc
    62401 ttacatgcgg gggcggacat tggatgatgc attcgttatt ttggatgaag cacagaacac
    62461 gacagttgct cagatgaaaa tgttcctgac acgtcttggt tatcaatcaa aaatgattgt
    62521 caatggagat acgagtcaga ttgatctgcc tcgagggaca acaagtggtc ttgttcatgc
    62581 agaacgaaca ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt
    62641 tgttcggcac cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca
    62701 agaataagct ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa
    62761 gaaaaacttg gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc
    62821 ggcgttacat ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca
    62881 gaagaaagta tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga
    62941 aaactggcag cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac
    63001 cagaacaatc ggatcaagca actatttgaa ttgatcgata aaacgaatga tgccatcaac
    63061 aaatcttatg acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact
    63121 acagaagagc ggatcgccgc gttgaagaaa aattttgaaa atgtgaatgc agaaaatgtt
    63181 gcgttttatc aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact
    63241 gagatccata ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa
    63301 gttcgtgaaa gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg
    63361 aatgtcacac cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg
    63421 aatgacgtat tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta
    63481 caaccagtga tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct
    63541 tcagctatga aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg
    63601 ctaattgcca tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt
    63661 caattccaaa atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca
    63721 gtcagcatag tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc
    63781 ccgctgtttt atcccgctgc ttttgcacca cttgttttaa gctttttctt gaaccgaaga
    63841 gcagggatca tggctggtat tttccaagca gtatctgcac ttttcatttt ttatgattcg
    63901 attggcacga actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca
    63961 acgattgtca gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt
    64021 atttttcctg tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat
    64081 gcaacgactt ggttaatgct tgtctgcggg ttcactggtg cactcttctc attcttgctg
    64141 acgatcggtc tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta
    64201 aatgaactca gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt
    64261 acttatcatc atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc
    64321 ggacgttctt tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat
    64381 gctagctttt tcgttgagaa cttgccttca ggagcggaaa acccgcacaa tttcttattg
    64441 ccagaagaca gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa
    64501 gattataata tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta
    64561 atgcgttatt tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc
    64621 cgttatcctg gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt
    64681 tgtgaggctg cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc
    64741 cataatctta tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg
    64801 aaagaaatcc gaaaagtgga aaaatcattg atcagcggtt taagtagcac cttccattct
    64861 cggatcaaat atcctaaaat gaaaacagaa gttgagaaga taaaggaaga acaagaagaa
    64921 tagaaagaag cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga
    64981 agagaaaatg aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc
    65041 agaggaaaca gaaatgtctg ttactttcat ggacaatgcg gcaatccaag taatcaatcg
    65101 tgattaccgt ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg
    65161 cgaagacgaa atcccagtga tttttgaaga tggagagaat ccattgcctc gtgtactagg
    65221 agacatcatg atttctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata
    65281 tgatcgggaa cttggctttt tagcggtaca tggattcttg catatcaacg ggtatgacca
    65341 tatgacacca gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta
    65401 tggactcaaa agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg
    65461 ctgtccaagg agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt
    65521 tcggtatcct tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat
    65581 ggctgctttt agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa
    65641 atgtagtgga catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata
    65701 tggcagctgg agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat
    65761 ttgtccctaa aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca
    65821 taaatcaggg tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa
    65881 ccgaatcgtg ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa
    65941 aatccaagga gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat
    66001 ccataaacca aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa
    66061 agaagtggat gcagtcttat ttatgatcag tgctgatcaa aaacgcggcc ggggagacga
    66121 ttttattatt gaacgtctaa aaaacgtgca ttctccagtt tatctgatta tcaataaaat
    66181 cgataagatc catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc
    66241 ttttgcacaa gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga
    66301 tgtattagtc agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga
    66361 tcatcctgaa tactttatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg
    66421 agacgaagtg cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga
    66481 taaaatccat atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat
    66541 cggcaaaggc ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa
    66601 tcttttagga gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga
    66661 caaaaaagtc tatctgcaag actttggcta ccgaaaagac gaatactgat attctgaact
    66721 tcttataggt taagtataga aataaactgg ataaaaatcc gcacgttgag aaaaacggcg
    66781 gaagatcgaa acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta
    66841 ggagggagaa tatgagccag cttgcagaat cgaaaggctt gattttatct agccgtaatt
    66901 acaaagaaaa agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt
    66961 atatcaaagg aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg
    67021 aagcagtcta tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag
    67081 acgtccatgc gtttcggaac atccaagaag atatcttttt gagcggttat ggtacctatt
    67141 tgctaaatct agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat
    67201 ttacgcaaca agctctgcag cggatggatc aaggagatga tgcagaaatc atcacgaata
    67261 tctttgaagt tcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg
    67321 tatgcgggga aacaaaagga aaatttgact attcttccaa atctggcggt gtgatatgcg
    67381 agaaacactg gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg
    67441 ttcgaatgtt ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa
    67501 cgaagcaggc aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc
    67561 taaaaagcaa aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag
    67621 agaaaaatat gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa
    67681 aaaaatcaaa actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa
    67741 tagatcgttc actgagagga tctattatat aaacaagaaa actgcaaaat gaatacgtac
    67801 attgaccaag atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac
    67861 ccaaacgatt tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt
    67921 cggaagtagg gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac
    67981 tttttgtcgt tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc
    68041 aaaacagatg agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg
    68101 gtcatcaaac ggctgcatgc tgatgcaagc ttatgacaca gaaaaaggag caggtacgat
    68161 gagcccatat acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga
    68221 gccatcccgc cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca
    68281 tcatcaattt caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga
    68341 aagcttgcgt cttttaggaa ttgatccatt agaacacgat attcgttttg ttgaagacaa
    68401 ctgggagaac ccatcgatgg ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat
    68461 ggagatcaca caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac
    68521 ttctgagatc acttatggac tagaacgtct tgcttcatat attcaagaag ttgaaagtgt
    68581 ctatgatctg gaatgggctg atggtgtgaa atatggcgag atcttcaaac aaccagaata
    68641 tgaacattcc aaatattctt ttgaagtaag tgaccaagac ttgctactaa gcaactttga
    68701 ccgttttgaa aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga
    68761 ttatattttg aaatgcagcc atacattcaa tttattagat gctcgcggtg cagtatccgt
    68821 aacagaacga gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat
    68881 ctttgtggca gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc
    68941 agaaaacatg aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg
    69001 aagaaatgcc tgctcatgtt gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa
    69061 aatttttaga tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc
    69121 gcttagcaat caaagtaaca aacattccag aacgtcaagc agacagtgaa gaagaagtca
    69181 aaggaccagc caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag
    69241 gatttgtccg cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg
    69301 tggaatatgt ctatgtaacg aaatacactc acggcaaaga agcgaaagaa gtattgacag
    69361 gtttagcaga tgtcattaaa agcctcactt tcccagtaac gatgcattgg gcagaatacg
    69421 attttgaata tatccgccct attcactgga tcgtggcatt gttagatgac gaagtaatcc
    69481 cgtttgagat cttagatgtc aaaacaggca gagcctctcg aggacatcgt ttcttaggcg
    69541 aagatgtgac atttgcgcat gcaaaagaat atgaagaaaa attgaatgaa caattcgtta
    69601 ttgcttcacc gcaaaaaaga caagaaatga tcgttacaca agcaaatgaa ttagccgaaa
    69661 aaaatcactg ggttattgat ctagacgaag atttactaga agaagtcaac aatttagtcg
    69721 aatatccaac tgtcttttct ggcgacttcg aagaaaaata tctttctgtt cctgaagaag
    69781 tactagtcac ttctatgaaa gaacatcaac gttatttcga ggtacggaac gatcaaggta
    69841 tgctgcttcc tcattttatt tctgctcgta atggtgatcg ggtaaaattg gaaaatgtgg
    69901 ttcgaggcaa tcaaaaagta ttgacggctc gtttagaaga tgcggaattc ttttataatg
    69961 aagacaagaa agcttcaatc gaccaatacg ttgaaaaact aaaaactgtt actttccatg
    70021 aaaaaatcgg tagtatctat gagaaaatgc agcgggtaca agtcattgcc cgtatcattg
    70081 gcgaacaagt aggactatct gaggaagaat tgatggatct gcaacgagct gctgaaatct
    70141 ataaatttga tctagtaacc aacatggtag gggaatttcc tgaactacaa gggatcatgg
    70201 gcgaaaaata tgctctgctg aaaggcgaga aacctgctgt agcaacagcg attcgtgagc
    70261 attacatgcc tatatccagt gaaggagagc tacctcagtc tacagtaggt tctgttttag
    70321 cgattgcaga taaattagat agcatttttt ctttctttgc agttgggatg attccaagcg
    70381 ggtcaaacga tccttatgcc ttacgtcgcc aagcatacgg tgtcgttcgg atcatcgaat
    70441 cgaaaaaatg ggcctttcct ttgtctgttc tgcaagaaac gattagtgat gttatcagta
    70501 aagatacgga tcgtttcggc attggtttat cagaagggca acaacaggtg atcgacttta
    70561 tcaaaggccg tctgcgccaa ttattgacaa cgaagaatat tcgtcatgat gtcatcgaag
    70621 ctgttttgaa tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca
    70681 agcagcatct tgcagatgaa gacttcaaac catcgatgga agctttgaca cgtgtcgtga
    70741 acttagcgaa aaaagcggag cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa
    70801 atgaagcgga gaaagaactg cacaaagcag tagaggcaat gaaaaaaagc tttgctgacc
    70861 aaacgatcaa cgaaaattat gatcagcttg taagccttcg cccactgatc gaaaattatt
    70921 ttgatcagac gatggtcatg gcagaagaac cagcagtacg taagaaccga ttaacgcaat
    70981 taagtcaaat tgctgccatg gctttgtcgc tagcaagttt agatcaaatc atcacaaaat
    71041 aatttctaat atgtaaataa accagagaat aggcacaaaa tagggctctt tgtcaataag
    71101 gactgatgag ttgtgcaaaa tcaaatctga gtcagaatga accacattct ggctcagatt
    71161 tt
//
LOCUS       DACDKJ010000008        66049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDKJ010000008 DACDKJ010000000
VERSION     DACDKJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.008"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IV328_000607"
     CDS             complement(27..725)
                     /locus_tag="IV328_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8936774.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IV328_000608"
     CDS             complement(718..2070)
                     /locus_tag="IV328_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8936775.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IV328_000609"
     CDS             complement(2293..3693)
                     /locus_tag="IV328_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8936776.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IV328_000610"
     CDS             3854..4591
                     /locus_tag="IV328_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8936777.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IV328_000611"
     CDS             4716..5195
                     /locus_tag="IV328_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8936778.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IV328_000612"
     CDS             5192..5959
                     /locus_tag="IV328_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936779.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IV328_000613"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IV328_000613"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8936780.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IV328_000614"
     CDS             complement(6862..7464)
                     /locus_tag="IV328_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8936781.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IV328_000615"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IV328_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IV328_000616"
     CDS             8145..8729
                     /locus_tag="IV328_000616"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8936782.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IV328_000617"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IV328_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8936783.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IV328_000618"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IV328_000618"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8936784.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IV328_000619"
     CDS             10630..10809
                     /locus_tag="IV328_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936785.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IV328_000620"
     CDS             10831..11856
                     /locus_tag="IV328_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8936786.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IV328_000621"
     CDS             12031..13275
                     /locus_tag="IV328_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8936787.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IV328_000622"
     CDS             13428..14375
                     /locus_tag="IV328_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8936788.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IV328_000623"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IV328_000623"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8936789.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IV328_000624"
     CDS             complement(15277..15519)
                     /locus_tag="IV328_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936790.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IV328_000625"
     tRNA            15640..15712
                     /locus_tag="IV328_000625"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IV328_000626"
     CDS             complement(15831..16469)
                     /locus_tag="IV328_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8936791.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="IV328_000627"
     CDS             complement(16592..17224)
                     /locus_tag="IV328_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8936792.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="IV328_000628"
     CDS             17575..17874
                     /locus_tag="IV328_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8936793.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18369..20534
                     /gene="acm"
                     /locus_tag="IV328_000629"
     CDS             18369..20534
                     /gene="acm"
                     /locus_tag="IV328_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8936794.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20806..21291)
                     /locus_tag="IV328_000630"
     CDS             complement(20806..21291)
                     /locus_tag="IV328_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8936795.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21771..22412
                     /locus_tag="IV328_000631"
     CDS             21771..22412
                     /locus_tag="IV328_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8936796.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22431..23066
                     /locus_tag="IV328_000632"
     CDS             22431..23066
                     /locus_tag="IV328_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8936797.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23084..23899
                     /locus_tag="IV328_000633"
     CDS             23084..23899
                     /locus_tag="IV328_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8936798.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24218..25654
                     /locus_tag="IV328_000634"
     CDS             24218..25654
                     /locus_tag="IV328_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8936799.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25843..28290
                     /locus_tag="IV328_000635"
     CDS             25843..28290
                     /locus_tag="IV328_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8936800.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28520..29587
                     /gene="rlmN"
                     /locus_tag="IV328_000636"
     CDS             28520..29587
                     /gene="rlmN"
                     /locus_tag="IV328_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8936801.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29780..30883)
                     /locus_tag="IV328_000637"
     CDS             complement(29780..30883)
                     /locus_tag="IV328_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8936802.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31436..31984
                     /gene="aac(6')"
                     /locus_tag="IV328_000638"
     CDS             31436..31984
                     /gene="aac(6')"
                     /locus_tag="IV328_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8936803.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            32530..34191
                     /locus_tag="IV328_000639"
     CDS             32530..34191
                     /locus_tag="IV328_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8936804.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34261..34608)
                     /locus_tag="IV328_000640"
     CDS             complement(34261..34608)
                     /locus_tag="IV328_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8936805.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            34692..35633
                     /locus_tag="IV328_000641"
     CDS             34692..35633
                     /locus_tag="IV328_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8936806.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            35634..36677
                     /locus_tag="IV328_000642"
     CDS             35634..36677
                     /locus_tag="IV328_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8936807.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            36683..37753
                     /locus_tag="IV328_000643"
     CDS             36683..37753
                     /locus_tag="IV328_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936808.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            37743..38696
                     /locus_tag="IV328_000644"
     CDS             37743..38696
                     /locus_tag="IV328_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8936809.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            38708..39718
                     /locus_tag="IV328_000645"
     CDS             38708..39718
                     /locus_tag="IV328_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8936810.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(40003..40824)
                     /locus_tag="IV328_000646"
     CDS             complement(40003..40824)
                     /locus_tag="IV328_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8936811.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(40921..41199)
                     /locus_tag="IV328_000647"
     CDS             complement(40921..41199)
                     /locus_tag="IV328_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936812.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            41467..43476
                     /gene="metG"
                     /locus_tag="IV328_000648"
     CDS             41467..43476
                     /gene="metG"
                     /locus_tag="IV328_000648"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8936813.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(43955..44542)
                     /locus_tag="IV328_000649"
     CDS             complement(43955..44542)
                     /locus_tag="IV328_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936814.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            44717..46039
                     /locus_tag="IV328_000650"
     CDS             44717..46039
                     /locus_tag="IV328_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8936815.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            46194..46970
                     /locus_tag="IV328_000651"
     CDS             46194..46970
                     /locus_tag="IV328_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8936816.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            46963..47532
                     /gene="rnmV"
                     /locus_tag="IV328_000652"
     CDS             46963..47532
                     /gene="rnmV"
                     /locus_tag="IV328_000652"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8936817.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            47525..48409
                     /gene="rsmA"
                     /locus_tag="IV328_000653"
     CDS             47525..48409
                     /gene="rsmA"
                     /locus_tag="IV328_000653"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8936818.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            48860..49489
                     /locus_tag="IV328_000654"
     CDS             48860..49489
                     /locus_tag="IV328_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936819.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(49613..50722)
                     /gene="ugpC"
                     /locus_tag="IV328_000655"
     CDS             complement(49613..50722)
                     /gene="ugpC"
                     /locus_tag="IV328_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8936820.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            50931..51353
                     /gene="mgsA"
                     /locus_tag="IV328_000656"
     CDS             50931..51353
                     /gene="mgsA"
                     /locus_tag="IV328_000656"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8936821.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            51676..52212
                     /locus_tag="IV328_000657"
     CDS             51676..52212
                     /locus_tag="IV328_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8936822.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(52252..54015)
                     /locus_tag="IV328_000658"
     CDS             complement(52252..54015)
                     /locus_tag="IV328_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936823.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(54018..55733)
                     /locus_tag="IV328_000659"
     CDS             complement(54018..55733)
                     /locus_tag="IV328_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8936824.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(56512..56736)
                     /locus_tag="IV328_000660"
     CDS             complement(56512..56736)
                     /locus_tag="IV328_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936825.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            56936..59704
                     /locus_tag="IV328_000661"
     CDS             56936..59704
                     /locus_tag="IV328_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8936826.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            59785..60204
                     /locus_tag="IV328_000662"
     CDS             59785..60204
                     /locus_tag="IV328_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8936827.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            60216..60686
                     /locus_tag="IV328_000663"
     CDS             60216..60686
                     /locus_tag="IV328_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8936828.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            60703..61482
                     /locus_tag="IV328_000664"
     CDS             60703..61482
                     /locus_tag="IV328_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8936829.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            61469..62290
                     /locus_tag="IV328_000665"
     CDS             61469..62290
                     /locus_tag="IV328_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8936830.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            62307..63356
                     /locus_tag="IV328_000666"
     CDS             62307..63356
                     /locus_tag="IV328_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8936831.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            63369..64373
                     /locus_tag="IV328_000667"
     CDS             63369..64373
                     /locus_tag="IV328_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8936832.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            65006..66025
                     /locus_tag="IV328_000668"
     CDS             65006..66025
                     /locus_tag="IV328_000668"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8936833.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        22102 a        11477 c        13458 g        19012 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
    18181 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat
    18241 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
    18301 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
    18361 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
    18421 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
    18481 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
    18541 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
    18601 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
    18661 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
    18721 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
    18781 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
    18841 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
    18901 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
    18961 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
    19021 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
    19081 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
    19141 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
    19201 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
    19261 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
    19321 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
    19381 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
    19441 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
    19501 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
    19561 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
    19621 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
    19681 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
    19741 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
    19801 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
    19861 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
    19921 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
    19981 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
    20041 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
    20101 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
    20161 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
    20221 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
    20281 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
    20341 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
    20401 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
    20461 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
    20521 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
    20581 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
    20641 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
    20701 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
    20761 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
    20821 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
    20881 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
    20941 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
    21001 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
    21061 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
    21121 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
    21181 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
    21241 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
    21301 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
    21361 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
    21421 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
    21481 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
    21541 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
    21601 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
    21661 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
    21721 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
    21781 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
    21841 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
    21901 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
    21961 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
    22021 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
    22081 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
    22141 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
    22201 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
    22261 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
    22321 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
    22381 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
    22441 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
    22501 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
    22561 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
    22621 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
    22681 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
    22741 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
    22801 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
    22861 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
    22921 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
    22981 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
    23041 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
    23101 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
    23161 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
    23221 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
    23281 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
    23341 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
    23401 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
    23461 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
    23521 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
    23581 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
    23641 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
    23701 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
    23761 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
    23821 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
    23881 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
    23941 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
    24001 acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc
    24061 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
    24121 tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa
    24181 gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc
    24241 atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg
    24301 gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac
    24361 atttaaagga acgaacggtg atttggcagt agaccactat catcgttata aagaagatat
    24421 tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat
    24481 ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat
    24541 cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc
    24601 acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac
    24661 tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt
    24721 gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt
    24781 gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt
    24841 catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac
    24901 gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga
    24961 cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat
    25021 gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct
    25081 agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt
    25141 caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc
    25201 agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac
    25261 aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag
    25321 ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac
    25381 agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc
    25441 catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac
    25501 agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca
    25561 agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca
    25621 aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac
    25681 ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa
    25741 aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac
    25801 ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg
    25861 cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa
    25921 atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa
    25981 ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac
    26041 caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag
    26101 ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta
    26161 ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg
    26221 acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt
    26281 ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga
    26341 agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa
    26401 ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa
    26461 ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc
    26521 gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca
    26581 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
    26641 gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa
    26701 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
    26761 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
    26821 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
    26881 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
    26941 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
    27001 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
    27061 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
    27121 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
    27181 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
    27241 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
    27301 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
    27361 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
    27421 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
    27481 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
    27541 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
    27601 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
    27661 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
    27721 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
    27781 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
    27841 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
    27901 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
    27961 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
    28021 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
    28081 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
    28141 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
    28201 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
    28261 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
    28321 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
    28381 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
    28441 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
    28501 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
    28561 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
    28621 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
    28681 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
    28741 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
    28801 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
    28861 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
    28921 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
    28981 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
    29041 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
    29101 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
    29161 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
    29221 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
    29281 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
    29341 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
    29401 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
    29461 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
    29521 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
    29581 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
    29641 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
    29701 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
    29761 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
    29821 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
    29881 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
    29941 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
    30001 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
    30061 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
    30121 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
    30181 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
    30241 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
    30301 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
    30361 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
    30421 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
    30481 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
    30541 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
    30601 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
    30661 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
    30721 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
    30781 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
    30841 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
    30901 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
    30961 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
    31021 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
    31081 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct
    31141 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt
    31201 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
    31261 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag
    31321 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
    31381 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
    31441 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
    31501 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
    31561 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
    31621 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
    31681 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
    31741 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
    31801 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
    31861 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
    31921 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
    31981 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
    32041 aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa
    32101 caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt
    32161 gaaaatggct ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
    32221 acaccgttta ttcagataag ggactgtgac aagacttttg tcacagtttc tttttgttta
    32281 ttctttttta tctttgtatg gcaataatag acaatataac gaaaattatt taatggcttt
    32341 tggaagacca tggaaaacct tgtacaaagc tattttttct gaaaaattcc ttgaaacctc
    32401 acgaaaaaat cattatttta tgaaaaagac aaaaaaaagt gttgattttt taataattat
    32461 tcattaaact cttaaacaac ttcagaaaat attaattatt ctgaaaattc caataaaggg
    32521 gtacgtaaaa tgaaaaagaa atttacattt ggtttggttg cggtatgcgg ccttgtactt
    32581 gctggatgtt atggcggcag ttcagccaca agcagtagtt cagcaaaaag cggttcagca
    32641 gatggaggag gcgtatttaa cttagtcgtg cctcaagaaa tgcctacagc tgacttatca
    32701 gttgcaacag atacaatcag ttttacagca ttgaataacg tttatgaagg aatttaccgt
    32761 cttgatgaag acagcaaacc acagccggca ggagctagcg agttggcaga agtcagtgaa
    32821 gacggattaa cttacaaaat caaattacga gaagacgcaa aatggtcaaa tggtgatcct
    32881 gttacagcag cggattacgt ttatggctgg caacgaacag tagacccagc tacagcttct
    32941 gaatatgctt acctgtatgc gccagttgaa aatgcagaag acattacagc tggaaagaaa
    33001 gataaatcag aactagggat caaagcagtt ggtgactatg aattagaaat caaattgacg
    33061 aaacaaacac catatttcca atatctgctt gctttcccat catttttccc acaaaaacaa
    33121 tccgtagtgg aagaaaatgg ggatgcctat gcatctgcaa gtgataaagc tgtttataat
    33181 ggaccattca cattagaaga ttttgatgga ccaggaacag atacagaatg gacttataag
    33241 aaaaatgatc attactggga caaagataca gtaaaacttt ctgaaatcaa agtcagtgta
    33301 gtaaaagaat cttctactgc attgaactta ttcaaagatg gccaagcaga tgacgtgatc
    33361 ttgtctggtg agttagcaca gcaaaacgca aacgatccag cttatacttc tgttaaagaa
    33421 gcgcggacaa gctatatcga attcaatcaa cgtgaaaaga attcaccatt caacaatgtg
    33481 aacctaagaa aagctatttc ttattctatc gatcgtgaag ctttagtaaa acaagtacta
    33541 ggtgacggat cagttgtctc tacaggattg atcccatcag atatggtaaa aagtccaaca
    33601 acgaacgaag actttgcaaa agaagctgga aaattagttt cttatgacaa agataaagca
    33661 aaagaatatt gggaaaaagc gaaaaaagaa ttgggcgttg attctttaga atttgatttg
    33721 atggcttctg atgatgattc atcgaaaaaa gtaatcgaat atgttcaaaa ctcaatccaa
    33781 gaaaacttgg atggcgtaac agtcaaacca acacctgttc cattctctgt acgtcttgac
    33841 cgctcaactt cgggtgattt tgacgcagta ctaggcggat gggctgctga ctatgcagac
    33901 ccaagcagct ttactgattt gtttgtcaca ggtaattcat acaaccgtgg tcgctggagc
    33961 aatgcagaat atgacgaagc agttaaagca gcttcatcaa cagacgctgg cgatgaacaa
    34021 gcccgttggg atgatttcca aaaagcaatc aagatcatct ctgatgatat gggtgtcgtt
    34081 ccggtgtacc aaaaagcaga aggtcaccta gtgaatgaaa aaatcaaagg tatcgttcac
    34141 catgcggcag gcgcttcatg ggattacaaa tggacatacg ttgaagaata atcgttaata
    34201 ccgtgagcat gagaaaaaac gaggctgcgg caaatgtcgc aacctcgttc tcatattcac
    34261 ttatcctaac aaagaaaaaa gcatgaaaat agcagaaaga gcaattagta ttccggcaat
    34321 gatcagccaa aagctgtaca taccgtagct ggcagacgag agcgaggaaa attctggttt
    34381 tttctttcgg ttccttgttc gtgcaagatg gaccgatcgc tgaaaatgat cgaagaaacc
    34441 cgaagaaaag acccataaaa aaccgccgat aatcagaaac ggcaatgacc ataagaatag
    34501 atcattagat aattgaatga gtgtaaagga atggttgatg agatttttta ttaaaataac
    34561 cagaatcagt agaaaagcta ctgcgtaggg gatagatttt tttttcattt gattgctcct
    34621 ttatttttgg ttcattatct aataatgtag cgaaacatct gtcagaagtc aaaagctagg
    34681 gggaaacatt agtgaatagt tatatcaaat atgtattaaa acgtgtcttt ttcatgatca
    34741 tcacgctttg gttaattgca acaatcacct ttttcttgat gcaattgctg ccgggcacgc
    34801 catatacgaa tcaggaacgt ttgagtcctg aaacaatcga gatgctaaat aaacaagtcg
    34861 gtttggataa accagttatc gtacaatatg ggatttatct ttctaatttg cttcaaggag
    34921 attttggaat ttccttccaa tttaaaaacc agccagtcgc acatttgtta gctggaagaa
    34981 tcggaccatc cttgcagtta gggttacagg caattatctt tggaacagtc ctagggacga
    35041 ttttaggaac gatctctgcc atgaagcaaa atacatgggc agatacatct tctacactcg
    35101 tagcgatatt aggtcgttcc attccaaact ttgtatttgc tgttttatta caatatatct
    35161 ttgcaatcaa attacaagtt ttgccaatcg caaaatggga tggctttgtg tacacaatct
    35221 taccaacaat tgcgttggcc atgtcgccgc ttgctgactc tgctcggttt atccgtacgg
    35281 aaatggtgga agtactgcat agtgattatg tggaattagc aaaagcaaaa ggattgagcc
    35341 gttgggaaat cgcctttaaa catggtcttc gaaatagttt gattccattg atgactttat
    35401 taggaccgtt agctgtagca ttgatgaccg gatcactggt ggtagaaaat atctttgcta
    35461 ttcctggtat cggggaacaa ttcgttaagt caatcacaac aaatgattat cctacgatca
    35521 tggctgtaac gatcctttac tcatttatgc tgatttttgt tattttgatc gtggatctat
    35581 tgtacggtct agttgatcca agaattcgtg tttcagaagg gagccgagga taaatggaaa
    35641 tgataccaaa gcgttatgaa acagttgcag atatccctgc ggatgaattt cttccattac
    35701 aaaaaaatac cgaagaagaa cgtgaacaga ttactgcacc ttctttgaat tttatccaag
    35761 attcatggcg ccgtttaaag aaaaacaaag ctgccgttat ttcaatggtt ttactattgg
    35821 tcatcatttt gatctctgtg atcacaatct ttgtttctcc tcatgatcca actgcccaaa
    35881 acgtggctta tatcaatttg cctcctcgta ttccgggaat caatatcgat ggattgaatg
    35941 gaaaagcaat ggttggtggt gaacttgttg ataaatatgc tcaagcaaac gtcccttcaa
    36001 acgtaaattt ctttttagga acagacggtt tgggacgtga tgtattaagc cgcttattta
    36061 tgggaatgag aatctcgttg ctgatcgcat ttattgctgc attactagat gtaacgattg
    36121 gtgtagctta cgggttggtt tctggtttgt taggcggacg tgtcgataac gtgatgcaac
    36181 gttttttgga aatcttatca ggaattccaa acctcgttgt catgattttg atgttggtcg
    36241 tttttgaacc aggaattttt tcaatcgtgg ctgctatggc catcacaaac tggataccaa
    36301 tggcacgtat cgttcgtgca caaacaatga aattaaaaga tcaagaatat gttttagctg
    36361 gattgacatt aggtgaatca agaatgaaaa ttgcattcaa acatattttg ccgaatatct
    36421 ctagtgtcat tattattcaa atgatgttta gtattccatc tgctattttc ttcgaagcct
    36481 ttttaagctt tatcggttta ggtcttacgc ctccgtcagc ttcattagga acgatgttga
    36541 gcgaagggta caagacattc ctgtatctgc cttatctatt atggattccg gcagtaactt
    36601 tatcggttat catgattggc tttaacttac ttgctgatgg attgcgtgat gcctttgatc
    36661 ctaaaatgaa agagtgaaga cgatgacgaa aattttagaa gtgaaagact tagaaatctc
    36721 ttttgatacg tatgctggaa aggtacgagc gattcgcggt gtcaattttc atttgaataa
    36781 aggagaaaca ttggcgatcg ttggggaatc tggaagtgga aaatcagtga cgaccagaag
    36841 catcatgcgc ttgctgtcaa gcaacgccaa tatagatgct ggtcaaatcc tctttaaagg
    36901 taacgatatc gttggaaaat cagaaagaga gatgcaaaaa atccgtggta aagaaatagc
    36961 gatgattttc caagatccaa tgacctcttt agatccgacg atgcccattg ggaagcaagt
    37021 cgctgagtcc cttagaaagc ataataaagt ttcaaaaaaa gagggacaaa aagctgcatt
    37081 agaattgctt aaattagttg gaatacccga agctgagaaa cgtatcaaca gttatccgca
    37141 tcagttctca ggaggtcagc gtcaacggat cgtgatcgcg attgcactgg tatgttatcc
    37201 ggaaatcctg attgctgatg aacctacaac agcgcttgat gtgactattc aagcacagat
    37261 tttggagctg ctgaaagaaa ttcagacaaa aatcgataca tcaattatct ttatcactca
    37321 tgaccttggt gtagtagcga atgtcgctga tcgtgtcgct gtcatgtatg gaggaagaat
    37381 cgttgaagtt ggaacatcgg aagaaatttt ctacaacccg caacatccct atacttgggg
    37441 actgcttggt tcaatgccga cattagatag tgcgaacgat cgattgtatg ctattcctgg
    37501 ttctccaccg gatttattga acccgccaaa aggagacgca ttctatccaa gaaatgaatt
    37561 cgcgatgaaa atcgatgcag aacaagaacc accatttttt gaactatcaa aaacacacaa
    37621 ggcagcaact tggctacttg ctccgcaggc acccaaagtg acaccgccag aagaaatcca
    37681 acgtcgctgg gctatcttta aagagaaaca aaaacatact tacggtcaag gaggaattgc
    37741 tgatgccaaa taaactactt gaagttaaag gattgaaaca gtatttcaat gtaggaaaaa
    37801 agaatgaagt acatgctgta aatgacatca gctttcacat ttatgaagga gaaacattcg
    37861 gtttagttgg agaatctgga agcggaaaat ccactactgg ccgtacgatc attcgcttga
    37921 atgaacctac tgatggtgag atattattcg acggtcaaga tataacgaag atcaaagata
    37981 aaaaaggttt gacgaaattc cggcatgatg ttcagatgat ctttcaagat ccatacgctt
    38041 cgttgaatcc tcgtatgaaa gtcagagata tcattgccga aggaattgat gttaatggac
    38101 tagctaaatc atctgaagag cgtgcaaaaa aagtcgatga tctattgaga actgttggat
    38161 tgaatccaag ccacggtact cgttatcctc atgaattttc aggtgggcaa cgccagcgta
    38221 ttggtattgc acgagcattg gcagtaaaac cgcgttttat catttgtgat gaaccaattt
    38281 ctgcactaga tgtgtcgatc caagcccaag tggtcaattt gttgcaagat ctccaaaaag
    38341 aacatcaatt gacctatcta tttattgcac atgatttgtc gatggttaaa catattagtg
    38401 accgaatcgg tgtaatgcat aacggcttgt tattagagat gggaacaagt gatgaaatct
    38461 ataatcacgg tgttcatcct tatacagaaa gtttgttatc tgctattcct ctaccagatc
    38521 cagaccatga gagaaaacgc agaagaatca aatatcaacc tgaaccagat gacggacaag
    38581 tacgtcaact tcgtgaaatc gcaccagaac attttgtcta tgcaactgag caggaagtcc
    38641 catattacgc aaaaaaacta aaacgacaaa aggaatctct tctggttgct aactaggagg
    38701 gacatagatg ctaaaagctt ttaaattccg tatgtatccg acagaagaac aaaaacagca
    38761 attgattcgt acttttggat gtgctcgatt tacctataac catttgctga aaaagcggca
    38821 gaaaagttgg caacaaacag gagtagccaa tttttcgctc acacccgcta cattaaaaaa
    38881 agaataccca ttcttaaaag aagtagacag tcttgcttta gcaaatgcac agcttaattt
    38941 ggatcgggct tttcgcaatt atttcaaggg acgtgccagc ttcccaaagc ttaaaacgaa
    39001 aaaaagtatg tggcaatctt atacgaccaa caaccaaacc cgtacgatct atctggaaaa
    39061 tggctactta aagttaccaa aacaaaaaga actaatcaag ataaatcagc atcggccagt
    39121 tgaaggatcg attcgctccg ctacgatttc tgctcgatat aatgaagaat tttatgtcgc
    39181 actactttgt gatgtttcgc cagtaaaaaa agaatcttta gctaaatgga tcgggatcgc
    39241 ctatcatccg aagacattga tccaaacatc tcgacctcta gaagtaactt taccaaaatt
    39301 tcaccagaca gaagaaaagc tgcaacatgc acagcggaaa ttgaatgtga aagtccgctc
    39361 cgctcaccat agaaagatca ggttagatca ggcaagcaat tatcagaaac aaaagagaaa
    39421 agtaatggat ctttatctga aacaaaagaa ccagcgagaa gattatcttg aacaattatc
    39481 tgggaaattg gtcaaacaat atgactactt atttgtagaa tcttttccaa aagaagaggc
    39541 acatgctgat ttttctattc acgactggca taaattaata acaaaattgc gttataagtc
    39601 ccaatggtat aataaaaaat ttcttttgat caataccgac ggagcagaag aaagcaattc
    39661 cgtaagaaag agccaagtag tagaaaaaat gggcagacat tcggttatca agggataaaa
    39721 aatgaatgaa actgatagga gcgttagggt tagccttgtt aataaaaagt cttaatgact
    39781 tttgtttcta ggaatttttc ttttgaagaa aagctcacta attatgacaa accaataaaa
    39841 caatagcaga aaaagttcgt acgagctttt tctgctattg ttaatttttt attcagaaag
    39901 tttacgaata catcaaaatt ctgaaacaaa aaacgtaact cacaaagagt tgtttttgcg
    39961 ttttttcttg aatgattagg tctgaatctt ttgctcacaa ccttattcat ggtgccccgg
    40021 cttgagctga tagatttttg aaggacggcc gattcctaca ggacgttcac cgatctcatc
    40081 gaaatattgg agcatagctt tcttaaaatt ggaatgatcg atcgttttat aatcgatccc
    40141 taagaattta gcaaatactt ttcgagcttc cgtaatcgtg aaatctttac ctaaaacttg
    40201 tagaacttgc ggctcgtgtt ccattttatt gactacacgg ttgaatgctt tcaagatgat
    40261 ttgactatgg tcaaaagcca aagtatcttt gccagaagac tctccagtga ctaaatcaag
    40321 cgagatagcc acttctccgc tagataaatg gatcttgtta ctatgacgtt ctagtgtgaa
    40381 ccagcgtact tccttggcat catctccggc gattagcggt tcttctccaa taaaggctaa
    40441 gtagctgact gtgacgaccc agcctcgcgg atctcgatta ggcgtactga atgtatgcag
    40501 ttgctcaata ttttcttttg aaataaccac accagtttct tcctttgttt ctcttaagac
    40561 gctttctcca gttgactcat ccttttggac aaagccacca ggcaatgccc atgaatttcg
    40621 ataaggatgc ccttttcttt ggatcaacaa aactttcagc tgatcttctt ctttgttata
    40681 gcacatcagt acgatatcga cagtcaaaga gggcttgtca tattctggac gttcctgttt
    40741 ttgataccag ttaagaaatt cttcttcact tgctttatgt tcataatata atttttcttc
    40801 ttctttagaa gcaaaacggt tcatctgacc acctccattg acagtaaaag tactttttac
    40861 cttattgtag cagactctga aaaaaaacca aggatttact tgaaaaatct aggttatcat
    40921 ttaaatatga aaaataaatg gaagtatgag attgaataaa aagaaaagca gataaaacgg
    40981 caatacttta taccgctctt cacgaatggc aaagaagcca caaataacga tatatacgtc
    41041 aatgataaaa cgcaaaatcc aagctgctgt cataaaactg ttcaaatgcc catggaggtt
    41101 aaaataacca tacatacgaa tataaaaata gctgaatatt aaatgtgcta atgaaagaaa
    41161 agcaatacta agtgtaaaaa aaagatgttt cttctgcatg attgttccag tcccttacta
    41221 agatagatag tctttattat accacgtgtg aggggagaaa taaaaagccg ttttttagta
    41281 gatatgaaac aaaacaaaaa cccgcattta ttcaacctat tcatcctttt aaaggataga
    41341 ttgaacgtag atagaagtcg tttatccagt ctttttcatt ttgaaaaaaa gctaacagca
    41401 tgcttttttt atggtatact agcaaggaaa cgaattcgca tcatgcgata aaggaggaag
    41461 agccccgtgg cagaaaagga aactttttat atcactacgc caatctatta tccaagcggc
    41521 aaacttcata tcggcaactc gtatactacg attgcatgtg acgcaattgc tcgttataaa
    41581 cggttgatgg gctttgatgt gttttatctc actggagtgg atgaacatgg tcaaaaaatc
    41641 gaaacaaaag cagaagaact aggtgtaaaa ccgcaagaat atgtagataa aatggcggct
    41701 gatgtcaaga aattatggaa aacgcttgat atcagttatg ataaattcat tcgcacgaca
    41761 gatgattacc ataaagcagc tgttcaaaaa atatttgatc gtttattaga acagggagat
    41821 atttacttag gtgaatatga aggctggtac tctgtttctg acgaagaatt ctttacagaa
    41881 acacaacttg cagaagtgta caaagatgac gaaggtaacg tgatcggtgg taaagcgcca
    41941 agtggacacg aagtagaatt ggtaaaagaa gaatcatact tcttccgcat gagcaaatac
    42001 gcagaccgtc tattggaata ctatgaagaa catcccgaat tcatccagcc agaatctcgt
    42061 aaaaacgaaa tgatcaacaa ctttatcaaa ccaggattag aggatcttgc agtttcacga
    42121 acaactttta cttggggagt accagtcaag agtgatccga aacatgtggt gtatgtatgg
    42181 atcgatgcat tatccaatta tattactgca ctaggatatg gctccgaaga tgaaagtcta
    42241 ttcgaaaaat actggcctgc agatgttcag atggtcggta aagaaatcgt tcgctttcat
    42301 acgatctatt ggccaatcat gctgatggcc cttgatttgc ctctacctaa aaaagtattt
    42361 gggcatggct ggctattgat gaaagacgga aaaatgtcta aatcaaaagg aaatgtcgtt
    42421 tatcctgaaa tgcttgtgga acgttatggt ttagatgcac tccgttatta tttgcttcgt
    42481 gctgttccgt ttgggtcaga tggtgtcttt acgccagaag attttgtttc ccgtgtcaat
    42541 tatgatctgg caaatgatct agggaactta ttgaatcgaa cgattgctat gatcaacaaa
    42601 tattgtgatg gactcgttcc tgactatgcc tcacttgtca ctccatttga tagtgaactg
    42661 tctacgacgg ctgctaacgt tgtcggacgt taccatgatg caatggaaaa aatggaattc
    42721 aatacagcat tggctgaaat ctgggtgctg atttctcgtg caaacaaata cattgatgaa
    42781 acagaaccat gggtattagc aaaagacgaa gaaaagaaag ctgaattgaa tagtgtcatg
    42841 atccatttag ctgaatcttt gcgtatcgca gcgatattac ttcagccaat catgacagaa
    42901 acgccagaga agatcttcaa tcaactagga ttggactctg aaacaatgaa tcttgaagga
    42961 ttacactttg gcgaatttcc ttcaggaaca aaagtagttg caaaaggtac accaatcttc
    43021 ccacgtttag atatggaaga agaagttgct tttattcaag aaaaaatgtc tgaaggtact
    43081 caaacaaacg aagatacagt caaatgggat ccagaagaaa cagaacttgt ttcaacgaaa
    43141 gaaaaacaaa tcaaatttga tgtatttgaa aaagtagaac taaaagtagc agaagtcatc
    43201 aactgtcaaa aagtagaagg ggcagataag ttgctgcaat tccgactaga tgctggagac
    43261 agccaagacc gtcaaatctt gtcaggaatc gctgaatttt atcctgatcc aagcgaattg
    43321 atcgggaaaa aagtcgtcat tgtagcaaat ctgaaaccac gaaagatgcg tggacaaatc
    43381 agtcaaggaa tgatcctttc tgcagaagca ccagatggct ctttacaagt aatcgaagca
    43441 ccaaaatcca tgccaaatgg ctcagaaatc gcatagaaat tcaaaggggg tatgacacta
    43501 gttgtgtcat accccctttt tccgaaattt gagaagcaat tttcgtaaat tccttcttat
    43561 tacttgaagc caatcatata atacagaaac agagaactat ccaaaataaa tcctagaaaa
    43621 agaagtacga gcttcgctga tttttgttaa tttagaataa tactaatagg aatcgtaaaa
    43681 acagaaaaaa agcataatga taaagggtcg ggcagatttt aattgattca tctggtactc
    43741 ttttttattg tccttacatt cataaagttt gaatgcaatg actttattac atctatattt
    43801 atatctaaca gtagcgttga tgtgttaatt ataagttcta tcactttatg ttcaattagt
    43861 ttgtgctctt atatgtgtga ttgaaaatat agataaaaaa acaatcatcg gctttgactt
    43921 cgatgattgt tttctttatt ttgtaaaaat cctatcactt gattttatgg taacctttta
    43981 tttttgtctt caaacggctt ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga
    44041 cataccagaa aatcgtacgt ccgataccat aatgttgata gagctttgtc aacatcacgt
    44101 gtaacaagat caaccctaaa agcaaggagc cagctataaa ccaatgatta tcccaatgca
    44161 tgacttcact aagtgccatc actccaaaag gatagaaata aaaatagatc ccgactaagg
    44221 tcatccgcca gttctttgta aatgttccga tattcaataa aaagaaaatc gctgaaagca
    44281 aaataccgat aggcggaaaa agcccaacca aaaaggaata caacatcccc cacaagatca
    44341 tcacaggacc tttgatcaat gcaacgattg cgatgaacaa agtcagcaat attagttttt
    44401 gtgataacgt tgcaacagtc attaatccaa ataaaattaa tgtgaaccaa tgccaatggg
    44461 actgctgttg ttctttacgg gaaaaatcga cccggttgga taaataatct gctaatttcg
    44521 gataagaacg cttaaaatcc ataaactgtt cctccttcgt ggaaaaggta ggtttagtat
    44581 acgaaaaata aaaaagaaat acatccgtct taagagggag tttttcactg aaagtggaga
    44641 aaaaagaatg gtaaattaaa atattctcag ttatactgaa aattatacca aatttcaaag
    44701 aagaaagagg atgggaatga aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt
    44761 agctaccgtt atgggaaccg tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac
    44821 tgctagtact cagtctacaa gtttgacatt gctagcttgg cttttgggtg gtttcttaac
    44881 gatctgtgct ggattgacag tggctgaatt agctacagca atcccggaaa ctggtggagc
    44941 tgtaaaatat attgaagctg cgtatggaaa attgcccagc tttttattag gatgggcgca
    45001 aagcttgatt tactttcctg ctaatattgc tgcattatcg attatctttg cgacacaatt
    45061 gacgaactta ttacaattat ctaccgatta tcttttgttg attgcagtga tcaccgctgt
    45121 ttctgtgaca gggttgaatt tgttagggac aaaagtcggt gcttctgtac aatcagtgac
    45181 gttgattgtt aaattaatac caattgctgt gattgtcata tggggattat tgaccccagg
    45241 acaaggaact gtccagttat ttccaatcga agctggaaaa gatgttactt ttgtcgaagg
    45301 attgagcagt gcgttgttag caacgttgtt tgcttatgac ggctggttag gtgtcggtgc
    45361 aatggcaggg gaaatgaaac ggccggaaaa agatttgcct aaagcaatta ttcttggttt
    45421 gagttttgta acagtcgttt atctattgat caattttgtt tttttgaaaa ctttgccgat
    45481 tgatcatctt gccggtaatt tgaatgcagc ttctgaagca tcagacgtca tttttggagg
    45541 aatcggtgga aagctggtca ctattgggat cttgatctcg gtatacggtg cattgaacgg
    45601 ttatacacta actgggatcc gcgtgcctta tgcgatggct ttagaagacg agctaccttt
    45661 cagtaaacag ctgacaaagc tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt
    45721 ccagttagca gtagcttgta ttatgatgag ccttggttct ttcgactttt taacagacat
    45781 gttgattttt gtgatgtggc tattcagttt actgattttt atcggtgtct ttattttaag
    45841 aaaaaaagcg ccggaattgc cacgtccata taaagtacca ctttatccaa tcgttccgat
    45901 tattgcgatt ctcggtgcta ttttcatttt aggtatgacg atgatgacgc aaacgaaatt
    45961 agcattgatc ggtataggtg ttaccttgat aggtatccca gtctattatc aaaaaaagaa
    46021 aaaacgctca gaagagtaaa atatttcaac aacagacaga aaaagtacga actaggtcct
    46081 ctagatcttc gtgctttttc tatttcaata aaacaagttc ttctattttg atttttgcta
    46141 cttctgcaag tagtgcttat gtgctaaagt aagaatgaaa aggagagata aacatgatat
    46201 ttgattctca tacccattta aatgctgaac agttcaatga agatattcca gaaacgattc
    46261 agcgagcaca agaaatgggg gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga
    46321 ttgaaaagtc tttgcaatta agccatgact atccaaatat atacagtatt atcggctggc
    46381 atccgacaga ggctggcagt tatacgaaag aaatcgaaaa taaattacaa gaacaattga
    46441 ctttaccaaa ggttgtagca ttaggggaaa ttggcttgga ttatcattgg atggaagacc
    46501 cgaaagaagt ccaagataaa gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc
    46561 taccaatcag tattcatact agagaagcac ttgaagacac gtatcaaatc ctaaaagaag
    46621 aagatgttcg agatatcggt ggaatcatgc atagtttcag tggtgattat gaatgggcaa
    46681 aacgatttct tgacttaggg atgcatattt catttagcgg tgtcgtcact ttcaaaaaag
    46741 cattagacgt ccaagaggct gcagcaaatg taccaatcga acgattactt gtggaaacag
    46801 atgcccctta tctggcacca gttcctttta gaggaaagcg aaatgaacca ggctatactc
    46861 gttatacagt agaaaagatt gctgaacttc ggaatcttcc gacagaagaa gtggctgccc
    46921 aaacatggac aaatgcgcac cgattatttg gtctaagcga acatggataa gatgaagatc
    46981 caagagatta tagtcgttga aggaaaagac gatacaagaa ggctgcggga agtagttgat
    47041 gcagatacga tagaaacaat agggtctgca atcaatgaag agatcctgat gcaaattgaa
    47101 catgcccaag agactcgtgg agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa
    47161 ataagaaaaa tcattatgga agctgttcct caggcaaaac atgctttttt gccgcgcaca
    47221 caagctaaac caaagaaaaa gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt
    47281 ttagaagcat tgaaaaaggt cgtgacgcca gacatttccc aaacggctgt ttctgaaatc
    47341 actagacaag accttttaga ctatggttta atagctgggg cacaagcaaa agaaaaacga
    47401 gaaaaactag gtgacgagtt gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt
    47461 ttgaagatgt ttcgtattac gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa
    47521 caacatgagt gaatatcgtg aaattgcgac accctctaga acaaaagaga ttttattgaa
    47581 acatggtttt tcatttaaaa aaagtttagg acaaaatttt ctgactgaac caaatatttt
    47641 aaggaaaatc gtagaaactg cgcaaataga tgaacatacg aacgtgatcg aagttggacc
    47701 aggaatcggt gcattgacgg aacaattagc caagcatgca aaacaagtcg tagcatttga
    47761 gatcgatgat cgtttgatcc ctgtattggc tgatacgatg caaccctatg acaatgtgac
    47821 tattgtacat caagatattt taaagaccga tttgtcaaca gctgttagag aaacatttca
    47881 tgaggagctt ccgttaaaag tcgtagcgaa tttaccttac tatattacga caccgatcat
    47941 gatgcatttt cttgaatcag atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga
    48001 agtagctgat cgtatctctg ctgaaccagg aacgaaagct tatggttcat tatcgattgc
    48061 tgttcaatat tatatggaag ccagtttagc attcatcgta ccaaaaacag tatttgtccc
    48121 tcagccaaat gtagattcag cgattttgaa actgacaaga agagatatac cggcagtcga
    48181 agtgacagac gaaaaagcat ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa
    48241 aacgctttgg aataacttgc agcattcgta tggcaaagat gaccagacaa aagcatggtt
    48301 agcaaaaagc ttggaaacag ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt
    48361 acaagaattc gccgcattaa gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt
    48421 gtgacagaag ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
    48481 agttcctcat gtttttcgtt tgtttgggct tgttaccgca gatgcaagct tttgaacacc
    48541 gtttaagaaa gaggttgtgg gcctgccgtt taactctaag tcacaaggaa caatttcaaa
    48601 aaacagcttc tcatgttttt gagttagttg gtcttattac ttgatgcaag cttttgacca
    48661 ctattatgcc gaacaaggag atgtgacaca aattttgtca catctcctat ttttgtttct
    48721 tgatcaaaaa attcttatac ttgatactca cactcatttt gtatggattt agaaaagaat
    48781 atgtgtcttg acagttatta gtaagtgagt tatactaagt cgacatttat gtgtcaagac
    48841 atattaggag agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga tttctgcttt
    48901 attgattggg atgggggtca ttatcccaat ggtcatgcct aaaatcatga ttccaccagc
    48961 ttcctttact ttggcgtctc atgtgccatt atttattgcg atgtttttta caccaggagt
    49021 tgcagtggct gttgcgctag gtacgacatt tggtttcttt ttaacaacgc cggttatcat
    49081 cgcattgcga gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa
    49141 gcatccgagt atcgttttaa aaaatggaca gtttactttt gctaattggc gtttccaagg
    49201 gttcaatttt gtgatcggtg tgattcattc tttagcagaa atgctagtag tcagcatctt
    49261 ttactttatt ggtaatatgc ctgaaacata ttattctcaa ggatattttt atacagtatt
    49321 catcttgatg gggattggtg ggcttatcca cagtttagtt gattataata ttgcttattt
    49381 tatagcagga acactcagca aatattttga tatccccgta tttacggcag caaaaaaaga
    49441 aaatcggaaa gagtcgattc aagggtacgc gaagaaaacg acatattaga catgatctct
    49501 caaaaaaaaa actctcatct agtaatggat aataaatcct ctaagatgag agttttttgt
    49561 cctaaaaaga gctgcaccaa catcttctgg cacagctcta acagggggca atttatggaa
    49621 gtttgatgac ttgttctgtt tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa
    49681 ggtcgactat ttcgcctggt ttatggaaat ctcttgcatc tacttttgag atgaactcag
    49741 tagaaccagt ttttgtgtaa agcatcgttt cagctccaag taattcggaa acgacaactt
    49801 ctgatttcac tacggcttca ggtgaagctt caagggctac ttgttcactg tggatgtctt
    49861 ctggacgaat accgaagata acttcttttc cttcataacc tttttcaact aatactttgt
    49921 tttttccttc aggaatacga agcttaagcc catgtccatc tgtgataaca ccatttttca
    49981 atgtcacatt aaagaagttc atggcaggag atccaataaa gccggcaacg aacatattga
    50041 cgggtgtatc ataaacttct ttcggtgtac cgatttgttg gataaatccg tctttcatga
    50101 taacgattcg gtcagccata gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg
    50161 ttgtttcaag acgctggtga agtttggcaa tctcagcacg catggctaca cgtaatttag
    50221 catctaaatt tgataatggc tcatccatta agaagacttt tgcatcccga acgattgccc
    50281 gtcccaatgc cacacgttgg cgttgtccac cagataaagc agctggtttt cgtttcaagt
    50341 attctgtcag acctaagatc tctgctgcat tgtccacacg ttttttgatg tctgctttat
    50401 catatttacg taatttcaag ccaaaagcca tattatcaaa aacagtcata tgaggataca
    50461 atgcgtagtt ttggaaaacc atggcaatat cacggtcttt aggtgccacg tcattcatga
    50521 cttttccgcc gatatttagt tctccttcag agatatcttc cagtcccgcg atcattcgca
    50581 aagtagttga ctttccacaa ccggaaggac caacaaaaac gataaattca cggtctttga
    50641 ttgatagatt aaaatcagtt acagaataat tttctgcatt gtcatatttt ttatatacgt
    50701 ttttcagagc catttctacc ataatacgtt ccacactttc ttttttagtt ttattaacta
    50761 tttaaagtat aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa gaattttttc
    50821 ttttttttgt caatgtgcta gttttaagct ttaataaata tccataagaa ataaaaactg
    50881 atacactgga gtaaataagt atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg
    50941 ctttgatcgc tcatgaccga aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa
    51001 caatattgga aaaacatgaa ctttttgcta ccgggacgac tgggcagagg atcacggaag
    51061 caaccggctt gccgatccat cggtttaaat ccggaccgtt aggcggggat cagcaaattg
    51121 gagccatgat ttcggaggat cagttagatt tggtgatttt tcttcgagac ccgctggctg
    51181 cacagcccca tgaaccagat gtaacagcac ttatccgttt atctgatgtc tatgagattc
    51241 cgttagctac gaatatcgga acagctgagg tgctactaag aggactggaa gccggcttcc
    51301 tagattttcg aagaatcgtc catgaaatag acaagcatcc actttcattt tgatggataa
    51361 gcgtatggaa aaataacgtt taatgttcgc ctatatgtag ctataaaagc taaacaaact
    51421 gataatatgc gatataatac gaacgtattg gataatataa acaataatat tagtttttcg
    51481 tatttgcagt aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc caaaaataat
    51541 acagagtaga aaataaagcg ttaagtgcta agaggatggg atgttgcctc ttggacgaag
    51601 gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg atacgatgta gcagctctta
    51661 aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc tattatgggc
    51721 ttattgattg cattgatggt cactttgtcc cgactggtag caatcgagac cccatttatt
    51781 aaaatcagtg tcacatttat tccacaagtg atcatgggca ttctttttgg tcctttttgg
    51841 tcagggatag gcgcagtttt agctgattta gtaggaatgg ccctcttctc gaaatctgca
    51901 ttctttattg gatttacatt gaatgcattt attgaaggag caatctacgg attctttttc
    51961 tatcgaaaag agatcacttg gaaaaatgcc attctggcta cgcttagtgt cacgctgatc
    52021 atcaacttat ttttgacacc attatggctt gcgttgatgt accatgtgcc attgttcagc
    52081 tgggtcgttt gggcgccccg cctgttaaaa acagtaatat ggttaccgat tcaatcaatt
    52141 gccatttatt acgtaggacg tagtattcct tacaaaaaaa ttttaagaag tttagcgatc
    52201 cacgctaaat aaaaaacaag cagtcttccc atcccagaag actgcttgtt tttattgtgc
    52261 tgtgaattgt gcttgataga gccgtcggta ataaccatct ttttgaagca gctcatcatg
    52321 ggtgcccatt tcgacaatag ctccctgatc catcactaat atctggtccg catttttgat
    52381 tgtggacagg cgatgggcaa tcacaaaact ggtttttcct tccatcattg ctaagaaggc
    52441 ttgttggatt tttttctctg tcaatgtatc gactgagctg gtagcttcat ctaagatcaa
    52501 cataggggga ttactgatca tcgttcgagc aatcgtcaac agttggcgtt gtccatctga
    52561 tatctttaac ccttgaccgc caatcttagt atctaatttt tccggcaaac gttctacaaa
    52621 ttcatacata taagaggctt ttagggcatc attgatttct gcgtcacttg cttccggatt
    52681 cccaaaacga agattttctc gcaatgtact gtcaaatagc caagtatctt gtaacaccat
    52741 tccaaaactt ttacgtaatg tatcacgttg gatagagcgg atgtcatggt catcgactgt
    52801 aattgttcct tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg tggattttcc
    52861 ggcccctgtt tttcctacta tagcaatggt ttgacctgga taagcagtaa aattgaaatc
    52921 ttcgatcaaa cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag agacttttcc
    52981 tttgatattt ttgggttcca aggcataggc atctggcaaa tcaggctttt cttctggctg
    53041 atcgatcaaa tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat
    53101 tccagaaagt tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa agctggaaat
    53161 cacaccaaca gtgattgacg aaccactctt caatgccaat aggccgccaa ctaaaccaat
    53221 cgataaataa gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa
    53281 ttgtgcccgt tgtccttttt catatagatc agcatttagc agttcaaatt gctgttgact
    53341 agcttcttct tgctgaaaag ctttcacgat tttctggtta ccgattcgtt cggtcacaaa
    53401 gcccgagata tccccaataa tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc
    53461 aacgatccaa ctcacaagga aaataattgg tgtagaacaa agaacgacca atgtcagaag
    53521 tggacttaga cgcatcataa aaataaacga aagaatgatg attgccactc cagaaaataa
    53581 ttgattgaag gccgcagaaa ttgcaatgga aatattgtcc atatcatttg taaatcgact
    53641 gacgatattt ccgtgggagt tttgatcata ataacgaagt ggcaatcgat tcagatgagc
    53701 aaatgcatct tttcttaatt ttgcaacgga taagtaagct aaccgattgc ttaacacttg
    53761 gatgagccat tgactgacta cgttgattaa aacgatacca ccgaatagaa taagtaaatg
    53821 gaacagtcga ttgaagcgga cctgtccttc tccaatcatt tgatcgatac tttgaccaat
    53881 ttgtaacgtc atcacaactg ttgcaatccc attgatgatt cctagtatca cagctagcca
    53941 aatgtctttt gcataggcag taaggtaagg aatgaatcgg cggagcgtct gcaaggataa
    54001 tttttttact ttcataacta ttcttcctcc tgtgatgcga cgatttcctg atattctgct
    54061 gattgagcaa gtaattcttc atgggtaccc tgagcagcaa tcttaccttg atccatcact
    54121 aagatatgat gtgcttcttg aatagagcgg acacgttggg agataataat cactgtactg
    54181 ccaagataag tctgtagcgc cttacgcaaa tccaagtctg tttgataatc caacgcactt
    54241 aaagagtcat ctaaaatcag taatgctggc ttggcaatca aagctcttgc aatcgtcagt
    54301 cgctgttttt gtccgccaga gaaatttttc ccattttcca tgacttttgt atctaaacca
    54361 tttggaagct gacggacaaa ttcttctgcc tgagcgattt tcagagcttc ccaacattct
    54421 ttttctgtgg cgtctttctt accccattga aggttttcac gaatagtccc ggtaaatagt
    54481 acagaagttt ggggaacaag agcaatttgc tgacggagat gatccagctg ccaagaacga
    54541 acatccattc cgtccattat aactgtaccg gaactgacat catagaaacg cggaatcaat
    54601 tgtgtcaaag tcgttttccc gcttccggtt ggaccaatga ttccaaggac tgtatttcgc
    54661 ttcagctgaa atgtaatatt ttggagagaa agaccagaat caggagtata acggaagtca
    54721 acatggtcaa aagcgataat tgcttcagaa gaaaaatcgg gagtagtact gacctcagct
    54781 tcaagaatcg aattttctgt gtctagcacc tctttcaccc gatttcctga agcttcggca
    54841 cgtgtgaaga tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat ttgattcata
    54901 taattgatca aagcaagaac ttgtccttgt tgcaagctac caatatttac tttgatcccg
    54961 ccaactgtca gcaaacagat gatcccgaca ttcatgatca aggttgtagc aggtgataat
    55021 agggcagaga tattggaaac acgtaaataa ttgttcgcta gatcatttgt gcttttatcg
    55081 aacttctctg tttctttttt tgtctgcgca aatgcgcgga tgacacgaac accgctaaga
    55141 ttctgactaa cgtgttcatt gagctgatct agtttttctt gcactttttg gtagagagga
    55201 acggtttttt tgatgataaa aaataaaatg attgagaaaa taggcaataa tgctaagaaa
    55261 aacagaccta cttcccagtc gatcacaaaa gccatgacca ctgacccgat acttaaaaac
    55321 ggtgctctga tcaataaacg aatgaccatt gctaaagcta gctgaagctg attgatgtcg
    55381 tttgtcattc gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa
    55441 gtattgattt ttttgattag ctgattacgt aactctgtac caaatccttg tgaagctact
    55501 gaagcatagt attggcaaat gatagcacaa atcagaccaa tcacagacat agcgaccatc
    55561 cataagccca tttcaatgat ttttcctcgg tcattcattt ttagtccatt atcgatcaag
    55621 gaagccatca ttaaaggcag gacaagttcg aagacagctt caagaaattt gaagaacggg
    55681 cccaaaatga tttgcttacg atagtctttt gcataacgta ataactgaat catatgattc
    55741 ctcctattat ttatttgtcc acaattgaaa aaacaagtac taccacattg tagcttagga
    55801 aatttagaat gaaaagagaa caagcctttt cataaatgaa agttttatta tgaagaagct
    55861 ttagaaattg aatagactat tttttttaaa aaaaagaaaa tgagaaaaca tgccagtctc
    55921 ttcattcata ggaacgtcta ttttacttaa tttttctata aatgaattct gcataaaaaa
    55981 tatatcaagt aaatgtttat agatgaaact ctatttactt cttttttaat ttgttcttgc
    56041 aatctttttt gctacgtaat ttttgtaaaa aagtgggttg agagaaatct aattgatttt
    56101 caaagaagga gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag
    56161 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
    56221 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
    56281 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
    56341 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
    56401 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
    56461 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
    56521 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
    56581 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
    56641 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
    56701 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
    56761 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
    56821 aattataagg gataagtagg ctgctgtcta taataagata tatttttcaa ttctttatta
    56881 aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga ataagttgtt
    56941 gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt tagaacatat
    57001 gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt
    57061 cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca attctcgacc
    57121 agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac gcacgaattt
    57181 ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac caaaagactt
    57241 attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac aaatcaaaac
    57301 agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa gtggcacagg
    57361 taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc tgctagaaga
    57421 ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg aacttttgac
    57481 tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa ataaaaaagg
    57541 ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc gcttaacacc
    57601 agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc gtatgggaga
    57661 acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg aagagattac
    57721 tagtcatttc ttaacaactt tcattcgaag aatacctatc cgaatcgaac tacctgcact
    57781 taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc tcgaagaaca
    57841 aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt taacaagtca
    57901 gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc
    57961 atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga
    58021 actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata ttacgcaaga
    58081 ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa tcgttaaaag
    58141 ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt cttgcgaagg
    58201 aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga ccagtagaca
    58261 agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct ttcgacaaat
    58321 ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga gttactatct
    58381 ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc aactaatcaa
    58441 agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg tccaacgaat
    58501 tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc ggattttact
    58561 gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat
    58621 tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc gtttattaca
    58681 agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc agattgcttc
    58741 agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca tattagtaga
    58801 catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg taccgctttt
    58861 gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa
    58921 tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag aatggcgact
    58981 gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca ctggaatcgg
    59041 tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt gtcagttgaa
    59101 aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc cattattttc
    59161 catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca atatcgacta
    59221 tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg aatggttgga
    59281 cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc gtaatttttc
    59341 attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga tgggagaaat
    59401 cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca atcccaaaaa
    59461 attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac aaatgccaat
    59521 cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt tatctgctat
    59581 taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca attctgagat
    59641 tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag aagaagaatt
    59701 ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt ttagtatgtg
    59761 taagtgaaaa gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa
    59821 tttgcttcag ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact
    59881 gtattcgatt gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt
    59941 atgaggtaca aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac
    60001 aacgaattct tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg
    60061 ccactattga ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc
    60121 cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag
    60181 aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc
    60241 atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta
    60301 ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag
    60361 ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga
    60421 aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat
    60481 ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa
    60541 aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct
    60601 tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa
    60661 aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc
    60721 ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt
    60781 tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg
    60841 atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg
    60901 attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga
    60961 gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg
    61021 aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga caattatgca
    61081 gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc
    61141 ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat
    61201 ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt
    61261 ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat
    61321 tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt
    61381 tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt
    61441 caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat
    61501 aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa
    61561 ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt
    61621 atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac
    61681 gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa
    61741 ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc
    61801 tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt
    61861 gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt
    61921 caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac
    61981 gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc
    62041 tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc
    62101 tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt
    62161 tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt
    62221 gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg
    62281 gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa
    62341 aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa
    62401 aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga
    62461 agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt
    62521 tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga
    62581 aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg
    62641 ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag
    62701 gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt
    62761 tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac
    62821 aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa
    62881 cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca
    62941 ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa
    63001 tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg
    63061 cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc
    63121 aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa
    63181 aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat
    63241 tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg
    63301 acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg
    63361 tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag
    63421 ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg
    63481 tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga
    63541 atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa
    63601 acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag
    63661 aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg
    63721 ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg
    63781 gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat
    63841 tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg
    63901 aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata
    63961 gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt
    64021 gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc
    64081 acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg
    64141 aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata
    64201 cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg
    64261 ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt
    64321 tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat taggatagac
    64381 tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta
    64441 aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta
    64501 taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt
    64561 agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag
    64621 cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac
    64681 aatctgtccg ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt
    64741 taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca
    64801 ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt
    64861 agactattgt aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac
    64921 acttaaaaac aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat
    64981 caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc
    65041 ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg
    65101 aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc
    65161 tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa
    65221 cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact
    65281 tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga
    65341 tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc
    65401 aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac
    65461 aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa
    65521 tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact
    65581 gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc
    65641 aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg
    65701 tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac
    65761 aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga
    65821 agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt
    65881 agagctagct caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt
    65941 gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat
    66001 ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatcgtc
//
LOCUS       DACDKJ010000009        63475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDKJ010000009 DACDKJ010000000
VERSION     DACDKJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.009"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(152..550)
                     /locus_tag="IV328_000669"
     CDS             complement(152..550)
                     /locus_tag="IV328_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8936834.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="IV328_000670"
     CDS             complement(547..1320)
                     /locus_tag="IV328_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8936835.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="IV328_000671"
     CDS             complement(1317..2000)
                     /locus_tag="IV328_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8936836.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="IV328_000672"
     CDS             complement(2167..3054)
                     /locus_tag="IV328_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8936837.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="IV328_000673"
     CDS             complement(3051..3920)
                     /locus_tag="IV328_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8936838.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="IV328_000674"
     CDS             complement(4057..5343)
                     /locus_tag="IV328_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8936839.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="IV328_000675"
     CDS             5616..6194
                     /locus_tag="IV328_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8936840.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="IV328_000676"
     CDS             6194..7123
                     /locus_tag="IV328_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8936841.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="IV328_000677"
     CDS             7120..8856
                     /locus_tag="IV328_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8936842.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IV328_000678"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IV328_000678"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8936843.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="IV328_000679"
     CDS             complement(9752..10261)
                     /locus_tag="IV328_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8936844.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IV328_000680"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IV328_000680"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8936845.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="IV328_000681"
     CDS             complement(11562..12653)
                     /locus_tag="IV328_000681"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8936846.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IV328_000682"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IV328_000682"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8936847.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IV328_000683"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IV328_000683"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8936848.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IV328_000684"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IV328_000684"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8936849.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="IV328_000685"
     CDS             complement(16616..17590)
                     /locus_tag="IV328_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8936850.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="IV328_000686"
     CDS             complement(17571..19454)
                     /locus_tag="IV328_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8936851.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="IV328_000687"
     CDS             complement(19472..21697)
                     /locus_tag="IV328_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8936852.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="IV328_000688"
     CDS             21812..22846
                     /locus_tag="IV328_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8936853.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="IV328_000689"
     CDS             complement(22913..23926)
                     /locus_tag="IV328_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8936854.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="IV328_000690"
     CDS             complement(24004..24192)
                     /locus_tag="IV328_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8936855.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24632..24760)
                     /locus_tag="IV328_000691"
     CDS             complement(24632..24760)
                     /locus_tag="IV328_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8936856.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24763..26136)
                     /gene="rlmD"
                     /locus_tag="IV328_000692"
     CDS             complement(24763..26136)
                     /gene="rlmD"
                     /locus_tag="IV328_000692"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8936857.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDELLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26247..27302)
                     /locus_tag="IV328_000693"
     CDS             complement(26247..27302)
                     /locus_tag="IV328_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8936858.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27312..28742)
                     /gene="gatB"
                     /locus_tag="IV328_000694"
     CDS             complement(27312..28742)
                     /gene="gatB"
                     /locus_tag="IV328_000694"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8936859.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28739..30211)
                     /gene="gatA"
                     /locus_tag="IV328_000695"
     CDS             complement(28739..30211)
                     /gene="gatA"
                     /locus_tag="IV328_000695"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8936860.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30211..30516)
                     /gene="gatC"
                     /locus_tag="IV328_000696"
     CDS             complement(30211..30516)
                     /gene="gatC"
                     /locus_tag="IV328_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8936861.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30622..32658)
                     /gene="ligA"
                     /locus_tag="IV328_000697"
     CDS             complement(30622..32658)
                     /gene="ligA"
                     /locus_tag="IV328_000697"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8936862.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32675..34909)
                     /gene="pcrA"
                     /locus_tag="IV328_000698"
     CDS             complement(32675..34909)
                     /gene="pcrA"
                     /locus_tag="IV328_000698"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8936863.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35064..36623
                     /locus_tag="IV328_000699"
     CDS             35064..36623
                     /locus_tag="IV328_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8936864.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36734..38665)
                     /locus_tag="IV328_000700"
     CDS             complement(36734..38665)
                     /locus_tag="IV328_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8936865.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38683..39597)
                     /gene="pfkB"
                     /locus_tag="IV328_000701"
     CDS             complement(38683..39597)
                     /gene="pfkB"
                     /locus_tag="IV328_000701"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8936866.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39594..40346)
                     /locus_tag="IV328_000702"
     CDS             complement(39594..40346)
                     /locus_tag="IV328_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8936867.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40505..40714)
                     /locus_tag="IV328_000703"
     CDS             complement(40505..40714)
                     /locus_tag="IV328_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936868.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40725..42053)
                     /locus_tag="IV328_000704"
     CDS             complement(40725..42053)
                     /locus_tag="IV328_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936869.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(42418..43005)
                     /locus_tag="IV328_000705"
     CDS             complement(42418..43005)
                     /locus_tag="IV328_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8936870.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43088..44338)
                     /gene="clpX"
                     /locus_tag="IV328_000706"
     CDS             complement(43088..44338)
                     /gene="clpX"
                     /locus_tag="IV328_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8936871.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44585..45874)
                     /locus_tag="IV328_000707"
     CDS             complement(44585..45874)
                     /locus_tag="IV328_000707"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8936872.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46028..46960)
                     /locus_tag="IV328_000708"
     CDS             complement(46028..46960)
                     /locus_tag="IV328_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936873.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47117..47776)
                     /locus_tag="IV328_000709"
     CDS             complement(47117..47776)
                     /locus_tag="IV328_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8936874.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47779..48804)
                     /gene="sppA"
                     /locus_tag="IV328_000710"
     CDS             complement(47779..48804)
                     /gene="sppA"
                     /locus_tag="IV328_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8936875.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48817..49107)
                     /locus_tag="IV328_000711"
     CDS             complement(48817..49107)
                     /locus_tag="IV328_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936876.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49516..51321
                     /gene="glmS"
                     /locus_tag="IV328_000712"
     CDS             49516..51321
                     /gene="glmS"
                     /locus_tag="IV328_000712"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8936877.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51393..52748)
                     /locus_tag="IV328_000713"
     CDS             complement(51393..52748)
                     /locus_tag="IV328_000713"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8936878.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52772..53941)
                     /locus_tag="IV328_000714"
     CDS             complement(52772..53941)
                     /locus_tag="IV328_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936879.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53938..54825)
                     /locus_tag="IV328_000715"
     CDS             complement(53938..54825)
                     /locus_tag="IV328_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8936880.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(55014..58706)
                     /gene="nifJ"
                     /locus_tag="IV328_000716"
     CDS             complement(55014..58706)
                     /gene="nifJ"
                     /locus_tag="IV328_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8936881.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            58905..59426
                     /locus_tag="IV328_000717"
     CDS             58905..59426
                     /locus_tag="IV328_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8936882.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(60125..60682)
                     /locus_tag="IV328_000718"
     CDS             complement(60125..60682)
                     /locus_tag="IV328_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936883.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(60861..61472)
                     /locus_tag="IV328_000719"
     CDS             complement(60861..61472)
                     /locus_tag="IV328_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936884.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(61585..61842)
                     /locus_tag="IV328_000720"
     CDS             complement(61585..61842)
                     /locus_tag="IV328_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936885.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(62439..62717)
                     /locus_tag="IV328_000721"
     CDS             complement(62439..62717)
                     /locus_tag="IV328_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936886.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(62751..63002)
                     /locus_tag="IV328_000722"
     CDS             complement(62751..63002)
                     /locus_tag="IV328_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936887.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        17688 a        13739 c        11219 g        20829 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattgat tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aactaaaaag actaagaaac aataacaaaa
    24601 gttaacactt cttagtcttt ctttagcacg tctatttatt aaagatttta gggccttttc
    24661 tcatgttttt acgatcattc ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt
    24721 tctttttgat caattctttc attttttctt tttggtccat ttttattctc ctttaacgaa
    24781 tgaagcgact aattcgatat gatgggtttg cgggaaaaga tctacaggct gaactttctt
    24841 cacggcataa cctgcttctg caaataactt agcatctctt gcaaaagttg ccggattaca
    24901 tgagatatag acgattcgtt ctggtgacat ttcgattgct gattcaataa aatgatgatc
    24961 taatcctttt cgtggagggt caactacgat cacagatggt ttgatccctt tttcttgcca
    25021 ttttttcata actttttctg cttttccgac ttcataatga acattttcga ttttattatt
    25081 taaagcattg aactgtgcat cttcaattgc ttctggaata acttccatcc catacacctt
    25141 atccactctg tctgctaaag acaagccaat cgtacctatt cctgaataag cgtcgatcac
    25201 aatgtctttt ttatctaact cggcaaactc gatggctgtt tggtacagat actctgcttg
    25261 ttgagtattt acttgataaa atgactttgc tgagattcga taggtttttc ctaatagctc
    25321 atcttcgata tagtctctgc catataaaac tttttctttg tctcctaaaa tcacatttgt
    25381 tttttcttca ttgatatttt ggatgaccga aaccacttct ggcaattctt catgaatagc
    25441 ttcagcaatt tccttggatt taaagaattt ttctttacga gtaactaaaa cgaccatcat
    25501 ttcatgggaa taatgaccac gacggataat gatgtgacga agctgtcctt catttttttc
    25561 ttcattatat cctcgtactt ggaaccgttg caagatatct ctgactactt tgattgcttg
    25621 atcgatgaca ggatcttgga tataaaaatc ttcgataggg actagatcat ggctattctt
    25681 tttgtaaaaa ccggtggcta actgtccatc gatacggcga acagggatct gtgctttatt
    25741 acggtagccg aatggttctg ccattccgac cgttggaagt acaggaactt ctggcatctt
    25801 agcaatcttc gccattacat gctctacttg tttttgtttg aagacaagct gttgttcata
    25861 cttcaaatgg gctaaaggtg cgatacctat gcgaagtaat gcattgtttt ctacaggctg
    25921 acgatcaggt gattgtgtaa taaatgtttc gacttttcca tatccaaact tattgcctgc
    25981 ttttactact agtatttcta cttcttctcc agggagtgca ttttcgataa ataatggata
    26041 accatcgatt tttgctaccc ccattccttc ataactcaag tcgacgattt cgactgtata
    26101 tcgttcgttt ttctttacta tttgctgttc tgtcatattt tgctccttta agcctatttt
    26161 tcaatcctac tgaactctac tataaatcag ctaattacac aactaaaacc ggcactgacc
    26221 ttactacgtt cagcaccggc ttttgattaa ttcttttgat taggtgtttc gtctgctatt
    26281 tttccgtcac cgtcgatatc ttcatccgtt aaacgttcga cttcttttac gaattctttg
    26341 ctgacaacct ctaattcttc ttcatctgta cctgtgattg cttcatcagg aatctgatcg
    26401 gtattggcaa agaactcaat gtgttgatga agattttgga aatgcatcgg tgcttccccg
    26461 ccgtattcac catctaagtt gatcattact ggtcgattga tatcatcttc tgtttcaacg
    26521 aacaaactgc ttgttttggt atagattatt cttggatcat cgacatgtcg accgccattc
    26581 agcatgagtg ctgcaatatg caagatctca aagatatttg ctgtcttcac aatgattaaa
    26641 gaaaacttcc catcatccaa tttggcatct ggcgcgatct gttcgaatcc tccaacagag
    26701 ttcgtcagcc ctaggaaaaa catcgaagca tttccgatat attccccttc atcatacttc
    26761 aagcgcattt tgattggctt tactcgaggg agcatctctg ctcctttcgc aagataagca
    26821 agataaccaa aaatactctt tagttctgat ggaacttcat aagtcaattc tgtcaaatag
    26881 ccacctgcag cgatattgat gaagtaactg ttatcagaaa ggccgatgtc catttttacg
    26941 gtttggtgtt tttttatcac ttctgctgct gctttaatat tatctctagg aatttttaat
    27001 gctcgagcat agtcgttcgt tgtaccagct gggatgatgg ccattttagg acggtgttca
    27061 agcggagcga ttccattaac cacttcgttg attgtgccat cgccaccagc tgccactact
    27121 agatcaaaac ctagttctgc gactctacat gcttcattct tggctgaatc tggttctggt
    27181 gtagttgcat aggcgctggc ttcaaatcct gcttcttcta atgcctgtag gatatccgcc
    27241 aaattttttt tcaataattc ttttcccgag gtggggttat aaattacgcg tgcttttttc
    27301 attttcagcc tctatcgttt cgctaattca tctttaagta gcttattcac gacaccagga
    27361 tttgcttgtc ctttagttgc tttcatgatt tgaccgacta ggaagccaac cgcacgatct
    27421 tttccatttt tgaaatcatc gacagattgt tggttgttat ccaaaatttc attaatgatt
    27481 ggcagtagtt tgttcggatc agataattgc acccaaccat ttttttcaac tacttcttgg
    27541 gcttctccgc catttgtgat caattcacgg aagacttttt tggcgatttt tgagctgatc
    27601 gttccatcgg cgatcaaacg aatcattcct gctaagttct caggtgtcaa ttttgtctca
    27661 gctaattcca gtttctcact gttcaagtaa gcagaaactt cacccatcaa ccagttagat
    27721 gcttgcttgg catctgcacc attttccaaa acagcttcaa aaaaatcaga catttctttt
    27781 gtaagtgtca ataccattgc gtcatactct ggaagtccaa actcacggat atagcgttcg
    27841 cgacgagaag ctggcatctc aggcaaacta gcttttactt tttgaatcca ttcgtcatcg
    27901 atcacgaatt tcgggatatc aggctctggg aagtaacgat aatcactaga cccttctttg
    27961 acacgcatca aaactgtttt attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt
    28021 tctcctcctg aaagaagaac ttttgcttga cgtttttctt caaaagctaa accttttttc
    28081 acgaaagata aagagttcaa gtttttcaat tctgctttcg taccgaactc ttcttgtcca
    28141 taagggcgaa gagaaatatt ggcatcacag cgcatagagc cttcttccat tttcacatcg
    28201 ctgacttctg taaattggat aattgatcgc aatgcttcta gataagcgta tgcttcttct
    28261 ggtgaacgca tatcagcttc agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa
    28321 tcaacgtaag aataaccgcc aataccatgc atgttttttc cagcatcttc ttctaaatgg
    28381 acacgttcga tccggatttt tttcttttta ccttctactt cgatttcgat ccatccgtca
    28441 tgaccgattg gttgatcaaa ttgggaaatt tgatatgctt ttggattatc tgggtaaaag
    28501 taatttttcc ggtcaaaatg agtatcttta gagatttcac agtttaatgc caaagctgct
    28561 ttcatcccga attccagcgc agctttattc attactggca gcacgcctgg ataaccccag
    28621 tcgatcacgt ttgtattgct gtttggttct gcaccaaaat gagctggcgc tggagagaaa
    28681 atttttgagt tggtttttaa ctcgacgtgg acttctagtc caatgactgt ttcaaaattc
    28741 attcgtcttt ccctcctaaa atagctggtt gttgtttatg gaattctgtt gcttgttcaa
    28801 acgcatatgc cgcttgatac atcgtttttt catcaaaata attaccgatg atctgcaatc
    28861 caactggtaa tccttctgat aaacctgccg gtacagacat acctggaagt ccagccaagt
    28921 tgacagggat cgttaagatg tcactcatgt acattgtgat tggatcgtta atgttctcac
    28981 cgattccgaa tgcaactgtt ggtgaagatg gcccgatgat caagtcataa tctgcaaaga
    29041 ctttttcaaa atctcgtttg atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt
    29101 cataataacc cgcgcttaag gagaatgtac ctaacatgat acggcgtttt acttcttcac
    29161 cgaatccttc tgaacgtgag ttgacatata catcatctaa tgatttcaca ttttcagagc
    29221 gataaccgta gcggatacca tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa
    29281 tgatataata aacagccaca ccgtattttg aatgaggcaa gctgacttct tccacaacag
    29341 cacctaatga acggaatgtt tctgctgctt tcaatacagc ttcttttacg ccaggggcca
    29401 caccttcgcc taagtattct ttaggtaaag caatcttcat tcctttgatg tcgcctgtca
    29461 gtccctctgc aaaatcagga actgaaacgc ctgaagaagt accgtctttt tcgtcataac
    29521 cgctgatagc tgtcaatgct aatgcattat ctttcaccgt acgggtcatt ggtccgattt
    29581 ggtcaagaga tgaagcaaag gcaatcaaac caaaacgtga gacacggcca taagtaggtt
    29641 tcatccctac gatgccgtta aaagctgctg gttggcggat acttccccca gtatcactac
    29701 ctaaagaaac tggaatttgt ccagctgcaa ctgctgctgc agaaccacca gaagagccac
    29761 caggtacttt tgttgggtcc caagcatttt ttgttttctt gaaataagaa gtttctgtac
    29821 ttccacccat agcaaattca tccatgttta gcttaccaac agggatcata tctgcttgat
    29881 agattttatc catgaccgtt gcatcataaa ttggttcaaa attgtaaagg atcttagatg
    29941 cagcggttgt caagatatct tttgttacga tattatcctt gataccaatt ggaataccag
    30001 caaggacgtt gctttcatca atcccttttt catctaaagc ttttgcctgt tccaatgctt
    30061 tttcttcgtt caatgtaatg aatgcctcga cctcaggctc tgtttctttg attcgttcaa
    30121 atgttgtttt tgtcaaatct tgtacagtga tttctttcga tacaagcata tgatgcagtt
    30181 cttctagaga acgattgtgt aattctgtca ttatgcgcca gcctccccat tatcgatgat
    30241 tgcgggtact ttgataaagc cgtcttctgt ttccggcaca tttttcaata attcatcgcg
    30301 gttccatcca gcttgcgccc gatcttgtcg catcacgttt actgtttcaa ccacattcga
    30361 cgtaaatgga acaccttccg tatctacttc ttccagcatt tcaaccatat cgatgatttt
    30421 acctaattgg tcggtaaaac ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt
    30481 ggcaacgtgt ttcacttgtt cttcactaat cgccatacta ccccttcttt ctaaacgagt
    30541 taaaaacttt gctctctatt ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat
    30601 ttgtctgaaa aaactcattt ttcattcttc tactgagcga cttttagcca atgcatctgc
    30661 catttgatct tctgtccaaa cttcgattcc taattcttgt gctttggtca gcttgctgcc
    30721 agcatcttct cctgctacca cgatatccgt ttttttcgat acgctaccag tcactttccc
    30781 gccaagattt tcgatcgtct cttttgcttc ttcacgagta aatcgtgtta gtttaccagt
    30841 aaggactact gttttttctt taaaaggaga ctcgacttct tgcaattggg catttcgaag
    30901 ccctttgtat tcgaaattga cgcctgcttg ttttaattca ttcatcaatt catggacttc
    30961 ttcgttactg aaataagtga ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc
    31021 aataatcgac tcatagtcac ttttactcaa tgtttctaga tcaccgaaat gttctgctaa
    31081 aatttttgct gctttcgctc cgacatgtcg gattcctaac ccgaaaatca accgctcgac
    31141 cgaattatct ttgctgttgt cgattgctgt caaaatatta tttgctgatt tttcttttat
    31201 tttgtccaac gtcaataact cttcttctgt caatttgtaa agatcagcaa catctgctac
    31261 tagtttttta tcatacatct gttctagtac gcgcggcccc aacccatcga tattcatcgc
    31321 atttcttgaa acaaagtggt tcaaaccttc tttcatttga gcagggcatt taggattgat
    31381 gcaccgtaag gctacttctt catccaaatg gacaagttca cttccacaaa ctggacaatg
    31441 tgtaggcaat tggtattctt cgctgtcttt aggtcgttta tctaacacga cttgcgaaac
    31501 ttctgggatg atgtctcccg ctttataaat cagaactgta tctttcaagc ggatatcctt
    31561 caacttaata taatcaccgt tatgcaggct ggcacggctt actgtcgttc ctgctactcg
    31621 gactggtgtc atgatagctg ttggcgttac tacccctgtt cgaccgactg tccattcgat
    31681 gttttcgatt agcgtttcaa cttcttctgg cggaaacttg taagctgctg cccatctcgg
    31741 tgctttgata gtaaaaccaa gttgatcttg tagcgagaat tcattcactt tgatgacgat
    31801 tccatcgatt tcatagggca aatccacacg tttgtcatga tattcttcaa tgtatgacca
    31861 cacttcatca atcgaatgac atagacgttt ttcccgattc gtgtggaatc caattttttc
    31921 tagctcttct agcgcatcat actgggtttt tgcttgcata ggaccgaaat cagctactgt
    31981 gtataaaaaa gtgtccagat ttcttttcgc tgtgatttta gaatctaact gacgcaaaga
    32041 accagctgcg gcgttccgag gattcgcaaa gatatctttt ccctctgctt ctctgtcttg
    32101 gttcaattgg acaaatgacc gttttggcat gaaacattcg cctcttactt cgatgttcat
    32161 tggttctttt aatttgattg ggaccgaccg gacagttttt aggttttcag tgatgttttc
    32221 tcctacagtt ccatccccgc gagtcgcacc tctcacgaaa tttccatctt cataacgcaa
    32281 agaaacggaa agtccatcta tttttagttc acagcaatag tcaactacac gtcccactgc
    32341 tttttgtaca cgttgatcaa aagctatcag ctcttctttg ctgaaaacgt cgttcaaact
    32401 gtaaagcgga atatcatgag tgactttttc aaaaccttct aatacttttc cgcctactcg
    32461 ttgagtggga gaatcagacg tgatcaaatc aggatattgt tcctcgattg ctactaattc
    32521 tgcgtattct ttatcataaa catagtcttc cactgttggt ttatcttcta cgtagtattc
    32581 gcgagaccat tggttcagtc ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat
    32641 cgtcatttca tgttccatca tttaccctcc actactaagc tttttcaatt ggcgcaaagg
    32701 cagccaaaag acgttttact ccttgacttg ggaaagccac atctagttct agatccttcg
    32761 acgtcccgct tactcggacg actgttccta gaccccattt tttgtgtttg actttatctc
    32821 caggtttcca gctgttattt tcccctccag tagcactttt agagggaaca accgctgtct
    32881 ctggctgttt atagctatat gctgtttttt tagcgaatac aggcgttttt ttaggcgtag
    32941 gacgcatgcc ttcgatttcc agtaactcct gatcgatttc ttctacaaaa cgagaaggtc
    33001 ggttatattg cgtccgtcca tataatgttc tggagaaagc atttgttaaa tacaacgctt
    33061 cttctgctcg cgtgatcccc acatatgcga gacggcgttc ttcctccagt tcgctttctt
    33121 ccatcaatgc tcgagataaa ggaaaaaccc cctcttctag accaatcagg aatacaactg
    33181 gaaattctaa tcctttggct gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt
    33241 ctaaattgtc aatgtcggaa acaagtgcca gatcatttaa gaagaccgtt agtttttctt
    33301 ctggtgcatc agcgtcttct tcattttgct gttcgaattg tttgtcgaat tcttgtgtga
    33361 ccgttaaaaa ttcctccaag ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt
    33421 ttaagtcttc tagatatccg ctacgatcca aaacctcttt cgtcaattct gtcactgtca
    33481 agtatgggat catctgagtg acttcttgga tcatctctcc aaatgcacct aattgctgtc
    33541 ctgcttttcc accgatattt gccagatcca cattttgcgc tgcttcaagc agtggccatt
    33601 catgaagaga agcaaaactg cgtagttttt cgatagatcc tggtccgatg cctcgtttgg
    33661 gcgaattaac gatacgctca aaactgattg aatcttgagg atttaccagc acattcaaat
    33721 acgctaaaat atctttgatt tctttacgat cgtagaactt gtgccctcca accattttat
    33781 aaggaatatt tgctttcaac agagtttctt ccattacacg agattgtgcg ttcgtccggt
    33841 acaagatggc aaagtcgccg taattccgat gattgcttcg aatctcttct tgcatccggt
    33901 ctacgataaa acgggtttcg tctcgttcat tatctgcacg gtagtaagtg atcttgtttc
    33961 cttctttgtt ttctgtccac aagttttttg gttttcgatt gctattgttt tcaatgactt
    34021 ggtttgctgc gctaaggatg tttttcgtgg agcgatagtt ttgttctagc aaaattactg
    34081 cagcatctgg ataatctttt tcaaaatcca agatgttctg catatctgca ccgcgccagc
    34141 cgtaaatact ttgatctgca tctcccacga cgcacaggtt cctaaatcta cctgccaata
    34201 aattgactag cgtatactgt gcgtgatttg tatcttggta ctcatctaca tgaatataat
    34261 ggaatttgtt ttgataatac gtgagtgaat caggatgttc ttcaaataat cgaatcgtat
    34321 tcatgatcag gtcgtcaaag tccatacatt gattatattg aagctctttt tggtaagcag
    34381 cataacattt tgccgcaatc tcttcgtaat aactgccttg catttttgca aaatcctgag
    34441 gggtctgcag gctgtttttt gcttgagaga ttgttcctaa tatacttcga ggatcatatt
    34501 ttttcggatc aatgttcagt tcttttagta tccgtttcat caaggtcagc tgttcagaag
    34561 aatcgatgat tgtaaagttc cggttatacc cgataaaatc aacatctctt cttaaaatac
    34621 gcacacacat cgagtgaaaa gtcgataccc agacatcttc gcctccagat gcgagaatcg
    34681 cattgacacg ttctttcatt tcttttgcag ccttattggt aaaagtaatc gctaggatgt
    34741 tccaaggatt gacttctttt tcctcaatca aataggcaat gcggtgtgtc agcactctcg
    34801 tctttccact tcctgcacct gccattagca gtaatggtcc gtcagtgtga agaacagctt
    34861 ctttctgacg ggggttcatc ccgtttaata attcagcttt ggatgacatt aacaacatcc
    34921 tttcttttat caatcttgtt aattatagca ttttcttggc tttcattaaa ctagctagtt
    34981 caaaatcatt tgtttctttt tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa
    35041 ttaaaagaaa gcgaggcgtt tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga
    35101 ctcagatata ttttattcta aaaggaatat ttgttggact gctgacagga atcgtcgtta
    35161 gcctgtttcg attatctatc gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga
    35221 gtcgggaaca gccgatttac ttaattggcc tagctatttg ctgcatcctt gcagcatttt
    35281 tcgtgggtta tctggtcaaa aatgaaccgg atatcaaggg aagcgggatt ccacaagtag
    35341 agggacagct tcgcggggaa ttatcaatga actggttctc tgttttatgg aaaaagttca
    35401 ttggcggtgt actttcagtt ggtgcgggcc tttttctagg tagagaagga ccttctatac
    35461 agcttggcgc atccattgga caaggagctg gacaattgtt tcggagtcca tcttcagaag
    35521 aaaagatttt aatttctagc ggtgcaagta gcggacttgc agcagcattc aatgctccta
    35581 tcgcaggatt actctttgtt ttagaagaaa tccatcactc cttttctcca cttgtttggc
    35641 tgacatcatt tgcttcagca atcactgcta acttcgtctc tctttacttt ttcggcttga
    35701 gacctgtttt atatctaggt gaccttcctt ctttgccgtt gaaatattat ggttccttag
    35761 tgatcttagg agtacttcta ggcatcttag gttttattta tcagaatgtc ttgctggctt
    35821 tgccaaaatt ttacaaccga ttgcctttac cagcttattt tcacgggttc gttcctttct
    35881 tgctgatttt gccaattggt tatttatggc ctagtactct aggcggaggc aatcaaatcg
    35941 ttttagcttt tggacatgct tccttgccaa tcattatgat tatcggtttg tttgttcttc
    36001 gattcgtttt ttccatgatt tcttacggtg ggggtcttcc aggggggatt ttccttccga
    36061 tattgacatt gggggcttta atcggtagct tttatggaaa cattatggtt gatttcggga
    36121 tggatccaat ctacatcaag gattttgtcg ttattgccat ggctggttat ttcacagcga
    36181 ttggaaaagc accgctgaca gcaattattt tagtgacaga aatggttggc tctcttaatc
    36241 atctgatgcc tttaggattg gctgctcttg ttgcttatat cgtcaatgac cttctcggcg
    36301 gtaatccaat ttacgaatca ttattggaac gtctacttgg aaatactcag accgcctcac
    36361 taaaaggtgc aaggacaact tttgattttc cagtaacggc agagagttct ttagacggat
    36421 taatggtacg agacttcaat tggcctaaaa atatgctgct aatctctata cgcagaggaa
    36481 atcaggaaat cttgactcac ggtgatacga tcatgaatgt aggagatatt ttggttatct
    36541 tgactgatga agcaaacttg ccaagaatca agcaggaaat cgaggctaaa tcttctatca
    36601 caaatttgaa gcaatcattt taacgaacat ggaaaaacta ttttacaaca actattcatc
    36661 tacactactt gattctatat gattctataa aaaaagaaga ggctgggaaa acccagcctc
    36721 ttctttttta tttctatgct tctgctactg caggagcttt tctcaataaa ccataaatca
    36781 caccagatac gactgctccg atagcaataa acaccaaata catgaccggg tgactcagta
    36841 agaatacaac gaaaatcccg ccgtgaggag ccagtaattt gattccgaaa gctccgacta
    36901 atccgccagt taaagcagaa ccaataataa aacttggaat ggcacgaatc gggtcagctg
    36961 ctgcaaatgg aatcgatcct tccgtgacaa acgatagtcc catcacgatg ttcgtcaaac
    37021 cagcatcttg atctgttttg ctgaatttat tcttaaacaa acgtgtagca acgaaaattg
    37081 ccaatggagg taccattcct ccagccataa cagaagccat cacgacactt ccgccagttg
    37141 caacagtcgt agctaaagtt gctgtaccaa acacataagc ggctttgttg attggaccgc
    37201 ctaaatctgc cgccatcatt ccagctaata atgcaccaag taaagcagca ttcgaaccac
    37261 tcatacttgt caagaaaccg ttcaatgcat cattgattgc tttcataggg acattgacca
    37321 gcaacatcaa gaaaccagta atcagtaagc caaacactgg ataaaagagg atcgttttaa
    37381 ttccatccaa tgattttggt aagttcttga ataatttacg taaaaatacg atgacataac
    37441 cagctaagaa tccaccaatc agtgcaccta agaatcctgc accgcctgta ttagctaatg
    37501 caccagcagc aaatccaacg atcaaaccag ggcggtcacc gatacttgaa gcaataaatc
    37561 ctgctaatac cggcagcatg aaaccaaatg cagcttgtcc gatttggttg aaccagctag
    37621 cagcttgatt gtaattccct agatttgata attgatcatg tggcacgcca ataaattggt
    37681 cgatcatgaa agaaagcgca atagcgattc ctccaccaat aacgaaaggt aacatgtgtg
    37741 atacgccgtt catcaaatct ttgtaaattc tttgtccgat agtaccatcc gcatttcctt
    37801 cttctgaact gctttctttt ccatcaccat ggaatactgg tgcagtacca ctaagggcta
    37861 gattgatcaa ctcttctggt ttacggatac catcactgac aggacgattg atcagttctt
    37921 ttccgtcaaa gcgattcatc tctacttttt tgtctgctgc aacgattaca ccttctgcac
    37981 gggcgatgtc ttcagcagtc aatcggtttt tgattccttc tgaaccgttc gtttcgactt
    38041 tgatctccac acccatttct ttcgcttttt tcttcaaagc atcttctgcc atgtaagtgt
    38101 gagcgatccc tgttggacaa gctgtaacgg caacaacgaa ttttctttct ggatgagcag
    38161 aagcttctgc agcttgtgtt tcttgttctg ctttttcttc tgcttctttt tgctcttcag
    38221 ctgtttggaa caagtcttgg acttcttctg gtgtatttgc ttcttttaat tttcctacga
    38281 aatctgcatc aatcagcaat cgagatagtg ctgctaatgc ttgcaaatgt gtgtcgtttg
    38341 cgccttctgg agctgcgatc atgaaaaaca gataagttgg ctgtccgtct aaggcttcat
    38401 aatctactcc tttttggctt tttgcgaata aaactgttgc ttctttaact gcactgtttt
    38461 tcgcgtgagg catggcgatt ccatcaccaa gacctgtaga agtttgtgct tcgcgggcta
    38521 gaattccttc tttatatgtt tcgatatcgg aaatccggcc ggcatcaaac attttttgga
    38581 ccatttcatc gattgcgcct tttttatcag tcgcttgcaa atccatgatc atcgcttctt
    38641 tgaccattaa atccttgata ttcatgatta tcctccttga aactaatcaa tttttgtgat
    38701 ttcaacttct ggcagcaact catcaatcaa ttcgcgagct gccaagtcat cagaaaaagc
    38761 tgttgcgctt ccgcaagcaa ctccccattt aaaggcttcg attggatctt tcgtacggct
    38821 gtaagcccca ataaagccag caatcattga atcccctgca cctactgaat ttttcaatgg
    38881 gcgaactaat acattcgttt tataagttcc ttgttccgtg aagagcaaag caccgtctcc
    38941 agccattgag atcagcacat tctgtgcccc ttcagctagt aattttttcc catacggaat
    39001 gatttcttct acagattgga aggttgtgtt gtaaagctca gctaattcgt gattgtttgg
    39061 tttgatcaac aacggtttct taacaagtgc attttttagg tcatcacctg ttgtatcaat
    39121 cacgaaatct gcaccttttt ctcgaatgat atcaatcagt tcttgataaa atccttttcg
    39181 caggctagct ggcgcgcttc ctgacaatac gacgatatct ccttgtccta ctgttcctaa
    39241 tgcttgcttc aactcatcga tttcttcatc tgaaatagat gggcctaagc cattgatctc
    39301 agtttcagtg tctgatttta gtttgatatt gattcttgta tcttctttta cggttgtgaa
    39361 caaagactgg atttgttctt tttcaagcca cttcgaaata aattcgccag tgaaccctcc
    39421 taaaaaccct agtgctgtag attgagaatc gatccgtttc aaaatacggg aaacattgat
    39481 cccttttcct ccaggtaact tgaagtcttc cttcattcgg ttcaaatcgc ctagttttaa
    39541 cccatctacg cgtacgatat aatcaattga tggattaagt gttactgtgt aaatcattgt
    39601 attgcctcct taattgttgt cttttcacgg aattcattaa cgaattctaa tggacaacgc
    39661 tcggttatga tcgtggctgc ttccaaaggt gcaacagagg tgaaggacaa ctggttgaat
    39721 ttggtatgat caatcaatac aaatgtctct tcggaatttt tcatggctaa acgtttaaca
    39781 gctgcctctt ctggatccgg agtcgtgaat ccttgttcaa gatgggcacc gttcattccc
    39841 ataaatgctt tattaaaatg aaaatttttt agttgttcta tcgcattaga tcctaaaaca
    39901 gcattagttg ccagctttat cgttccacct aaaataatgg tagcaagctc caaatcaact
    39961 aaatttgccg catgttgaac ggaatttgtc actactgtga tctgttttcc ttgcagaaaa
    40021 ggaatcattt ctaacgtagt agagccagcg tctaaataaa tgacatcttc gtcattaatg
    40081 gtttcagctg ccgatttggc gatttgctgt ttttcgtgaa cgtttttgag tgatttttca
    40141 ctcatattct gctcaaaatt caataattgc tctttcttag ctccgccatg aactctttct
    40201 agaaacccac tattctccag ctcttgtaaa tcacgtctta tcgttgattc agaagcattc
    40261 aaaaggttta caagatcttg tgatttcacg attttctgct gttcaagcaa gtctaggatt
    40321 ttttgtctgc gttcctctgt aagcattttc acgatcacct caatacaaat aatagcatat
    40381 tttttttcta ctagcaatat aaatcagtca aaaacgttca taaaaattat aaatcagtca
    40441 aaaaaaaata aaaaaccagt cagaaagact ggttttttat ttttttgtct atcatcaagc
    40501 cgttttatcg ttttttattt tcgcttcttg tttttttcat ataaacagaa gcaggttttc
    40561 tttccttatg aagaagataa taataaagtc ccatcccgcc aaacatgacc aaaaaaccga
    40621 tgactgatcg aataggaaaa ttaggaacaa aaaagagaca gacggataat aatgatgccg
    40681 gaaccattat tcgataatag agttgtttat ccattttctt cacctcatat tttttgtaaa
    40741 gtaatcaatt gttctaattc tcggtaatct aaaaattgca cacgcagatg ctgctcacct
    40801 ttcttgacat cgaattggat acttcgccgc tttttcttta ttcgtttgaa ggtaacatca
    40861 gagagtctat gtttgatttc ctttaacggt aattctgaca gggattctga aggaacatag
    40921 attcttaagg ataaaagata ctcttgatat ttttcatctg ttacctctac ttctagatat
    40981 tccctaatat aataacaata agcttcgatt ccattatcca aagcaacagt cggatcgata
    41041 actttatagc acatcatgcg tgatcgattc tcgtcaaccc aaggaaaata cgcctgagca
    41101 tttagtgttc cgcgaacacg cctttcttta taatctcctt gttggatatc ataacttttt
    41161 gttacaaaaa atgaagcatt ctttttagga acaggttgca tttgtgcatt ctgatactgg
    41221 acagattgga tcgtatggtc agtgtaaatg tattcaacga atggcggttt caataaaccg
    41281 acaccattgt cccaagcaag atgatcaaac ccaagctggt ccctaaaata ggattgaaac
    41341 atttgattgt ttggcttttg ccagttaaag gggtctcgat aaagctggat acatatgact
    41401 tcgttttcta attccttcac tttttctact tgttggatgt cgagaaaaat cggtttaggg
    41461 atgatttgat gataatagcg actataatac atttcccaac acgaagtgaa acataaacct
    41521 tggtaaaaaa gatcgtatcc tggaaattgg ttgcaatcga ctactacttc cccgttttgt
    41581 tgtttcattt taggtaaatg ttcgatttca tcctttttct caaacaatag ccgttgagag
    41641 atctgttttg tattgtgata gataaactcg ctataagctc gcatataggc gaacacccca
    41701 tgagcaatca tttttttctg aatgaagtct cgaatcaaaa attgcttttc tttgaaaaca
    41761 gagatagaca atacattttt tatcaataat tgattatctt ccaagcgaaa actacattcg
    41821 ttctcgtcat ttcctacaac taaccaactt tcgtctctta ccgatgctag cattattttt
    41881 tcttgaagct ttcgaagatc atacggatcc gcttgttgtg tgaaagagcg gataaatgtt
    41941 ggctggatca acagacgtgt caaaatagcc caatcgctta tttcctgttt tttttcttct
    42001 tcatttaagt ctgtgagctg gatagtcatc aataatttat cttcgccaat cattttctca
    42061 cctctttcaa atctttttat aatttaatta taaccaatga taaagaaaaa ctaaaacttt
    42121 atgtcttaga aaccaatttg gacgattaca tgatagacta agtaaaaaga aacaaagagg
    42181 tgtttacaat ggggttgaaa tttgaacgta cagataattt agataaactt ttcgaatcat
    42241 ttgctgtcga tccggaaaaa acagttgcac caaaagaaga gatcgatcga tttctaaaac
    42301 cacagccaca aaatgaaaag aaaaaagaca agaaacaaaa gtaagttaag ctcaaaaaaa
    42361 gacttaccag ccaaactttt atgaaagagt ttgactggta agtctttttt attcttttta
    42421 tgattcaatt gcttgttcga ttgctgccca cgcttcttct tttccctgtt ttgtaacgga
    42481 agaaaagaga ataaacgtgt cattgcgatc aaaatccaac gcttttttga tttcgctctc
    42541 atgcttgttc catttaccac gagggatctt atctgcctta gtggcaacaa caattacagg
    42601 gatatcataa tacttcaaaa attgatacat ctggatatct tcttgagaag gcttatgacg
    42661 caaatctact aaagaaacga ccgcacgtag ctggtctcta gtcgttaaat atgtctcgat
    42721 catctttccc cattttgctc gctcagtttt ggatactttt gcatacccat atcccggaac
    42781 atcgacaaaa tgtaatgcgt tctcgatcaa ataaaaattc aatgtctggg tctttcctgg
    42841 cttaccggaa gtacgggcta aattctttcg atcaatcaat gtgttgataa aagaagattt
    42901 ccctacattt gatcgtcctg ccagagctat ttctggcaat tgtgtatctg gatattgttt
    42961 tggtgccact gcacttatga taatttcagc ttgatggact ttcatggtct ctctccttaa
    43021 caaggtgatg aataaagcaa gcggctgtcg caaaagccaa aatgactttt acggcagcct
    43081 cttttcatca acccgctttt ttctctgaat ctttatagat tactgttggc tcaccagaca
    43141 attcagctgc ttcttttgtg ataatcactt tttcgatgtt ttcatttgaa gggatatcaa
    43201 acatcacatc catcatgatt tcttcaatga tggaacgaag tcctcgagca ccggtattgc
    43261 gttcgattgc ttttttcgca atggctctca acgcttcagg ttcgaattca agttcggtat
    43321 tatctagaga aagaagtttt tgatattgtt tgactagcgc atttttcggc tcagtcaaga
    43381 tccgtaccag atcatctgtt gtcaatttct ctaaagctgc cataacaggt aatcgtccga
    43441 taaactcagg gatcaatccg aattttagta aatcttcagg aatgatatgc tgcatgacac
    43501 tttcgtcttc agaaagtttt tggttttgtg taccaaatcc aatcgtcttt tcgcccatac
    43561 gattcttcac aatcgtttcg atgccatcaa atgcaccacc tacgatgaac aatatgttcg
    43621 tcgtatcaat ctgaataaat tcttgatgtg gatgtttacg tccgccctga ggtggcacac
    43681 ttgcaacagt tccttctaga attttcagca aggcttgttg gacaccttcg ccggaaacgt
    43741 cacgagtaat agatacattt tcgcttttac gcgcaatttt atcgatctca tcaatataga
    43801 tgatcccttt ttctgcaagt tccacattgt aatcagcaga ttgaagcaat ttaagcaaaa
    43861 tgttttctac atcttccccc acataaccag cttctgtcaa acttgtcgca tctgcgattg
    43921 caaaaggtac attcaatgtt tttgctaatg tttgtgctaa aaatgtttta ccagaccctg
    43981 taggtccgat caagcagata ttgctttttt gcagttcaac atcatctgtg ttttcttgtg
    44041 cttgctgatt gacacgttta tagtgattgt atacggcaac agctaacgag cgttttgcgc
    44101 gatcttgtcc aatcacgtag ttattaaata cttcaagaat ttctagtggt ttagggactt
    44161 cagtgaattc gcgtacagct tcttcataaa attcttcatc aatgatttct ttacataggt
    44221 cgatacattc attacagatg tagacccctg gaccagctac gatctttttc acttcttctt
    44281 gggttttccc gcaaaaagaa cagcgtacgg tctcattgct acttggattg tcatacatgg
    44341 tcttcacccc ttaacttgca aaaagtatgc aagaaactat caaagacttt acagtctttt
    44401 actatcataa catagtttag taaaaagaaa aagtgtttaa caaaggatca actgacctat
    44461 acctttgcat tttttactct taacaaaaaa taaggaactg tgacaaaatc gtgtcacagt
    44521 cccttatttc gaataaacaa ttttctagaa gtagcttctt cagaaatgat ttattacttg
    44581 atttctattc tttcgcttct gtcgctgtac ctgtgatcaa ttcgattgct tttttcattg
    44641 tcacatcatg ttccagcata tcttctgtca acacgcgttt gatttgttcg attggcatgt
    44701 tgtatgtttc tgataattct tggatttctg cattgatttc ttcttctgtt gcttgaagat
    44761 tttcagcagc tgcaattgct tcgatcacaa gatttgttct tgtgcgcatt tctgcttcac
    44821 cttcgaattg tttatgcaag tcttcttctg tagaaccagt taattgatag tacatttcag
    44881 gagagatacc ttgacgttgc atgttgttca agaattcgtc cattgaacga tgtacttcat
    44941 catgaaccat cacgtgtggc aattcaacga tttctgcgtt ttcaacagcc atacggatcg
    45001 ctgcttcatc ttttgcatcg tccgctgctt tttcttttgt ttctactaat tctttgcgat
    45061 atttttcttt caattcgtct aatgttgata cttcgtcatc aacatctttt gcgaattcgt
    45121 catctaattc aggcacttct tttgctttta cttcatggat tgtcactttg aagactgctt
    45181 cttttccagc aagatcttct gcttgataat cttctgggaa agttacattt acatctagac
    45241 tttcacctga tttatgacca actaattgat cttcaaaacc tggaatgaat gagcctgaac
    45301 ctaattcaag tgagtaattt tcgccttttc cgccatcaaa cgcttcgcca tctacaaagc
    45361 cttcgaagtc gattactact gtatcgccat tttcagctgc agcatcttct ttgataacta
    45421 attcagcttg tgcttcttgt tcacgtttga tacgtgcatc cacgtcagca tctgttactt
    45481 cacggtcttg tttttcaaca actagatctt tatattgtcc taattttact tctggtttca
    45541 ctgttacttc agcagtgatt acccagtctt gacctttttc catgctttca acatcgattt
    45601 ttggttgaga aacaggatcg atgccagctt ctttgacagc agcttcataa gcttctggta
    45661 atacagcatt caatgcatct tcatataaag cttcttcacc gtacatacgg ttgaatactt
    45721 gacgagaaac tttcccttta cggaatccag gtacgttcaa gctttttttc actttattaa
    45781 acgcagttgt taatcctttt tggatttcgt cttgcgcaat cgtaaatttt aaaacaccat
    45841 cattcgtgcc ttttttttca aatgttgcag tcatttaatt ccctccgagt ttagaaattc
    45901 attttataca tcaaaaacgt ttcatgcata cctttaaata gtacactaac cctttcaaat
    45961 tgtaaacata ttgaatggat agaatccttg tttttcataa catttttatc tttttcactg
    46021 ataaaatcta atggaagaag atcatttgct gcaaaatttt ttctcttttt tttaaaaaat
    46081 cttgtttttc atcttctgta ccttcataca tcgtttttcc tgtacacgcc tctacagtca
    46141 gttgaaacca gcgcttcggg tcttcgatgt atgtatcata gacaggataa acaattgcag
    46201 attgcaacgt gaattcttct tttaacacgg aactaagcag cgcatcttca ttcccgtaac
    46261 gatctgaaaa tacacataat tgctggtaac ttgcctgctg ctgcggcttt ggcagatctt
    46321 ttggtatgac tttgactaac ttttcttcaa tcgtcaaata gaaaacagga tggcattcgt
    46381 tcaattgcct aagtgtctcc agtaattttg cccttgctaa aatatggacc tctggcgata
    46441 caataagttt actcgctgtt tggataaact catgataagg aagctgttcg atctcatcaa
    46501 tagacgcaag ctgacgtgca ggttccaacg aaggaagttc ctccgattta ttgtggagtg
    46561 ccttgatctt ctgcctctcc atttgtccgg ataattcttc tgcaaattgg attttttcaa
    46621 tgacccgctg ctcgaaagct tcataaaaat ctgtacttgc tatcaatttt ctagcatata
    46681 aaaaatcttg tgtactgatt aacaagtcaa aataaaattc agcaaactcc tcattttgaa
    46741 gatactgttt ttcatgcaag agtgcttgct tcaatgcttc ttttttctcg tccaactcgt
    46801 aaagacaaaa aacaagcagg ctattagcct caaagctttc tttcaactcg taagctcgtt
    46861 gaaataattc ttttgcctgc tgatattgct ggtttgctag tgcttgttgt gccactcgca
    46921 ttaaattctc atatttttct ggaaaatcaa tcggcttcac gcagtgtcct cctcttgctc
    46981 atattcagct catgttcaat agtagcaaaa caaaaaaagc atgacaatta aaaataactg
    47041 aagcaccctc taaaagctag attttagatc tgacttttag aaggtgcttt caactgttac
    47101 tgctttttat tgctcatcag ctgttgactt cctggttgaa cgctttgatc agattgatcg
    47161 taacaacact tgtattagtg aaaaaatcgc ccagatgttg ctttttctga ttaaagactg
    47221 ctggaagata ccctaacatc agcaaaggaa attttagaat gaaccggcaa gcaccttctc
    47281 taactaaaac ggttgaccag ctaagttctt cttcatcaaa gctgatgact cgaataccaa
    47341 agatcatttt cccaatcgtt tggccatgat tgaatttcgt caataaaata aaatacgcta
    47401 gataaataag taaggctgtg aatccgtaga gactcagata agaatctgaa aaattaagcc
    47461 aacctagatt tttggcaata ccaagtgtta aacgcgtaat agctccgata cacaaaagat
    47521 cgaccaagaa ggcaaacatt cggataaaaa aacctgcata gaaatactta ggaaaatcat
    47581 ttgcttttgg cggttcattt cttgtgtccg tatacttttt ccatttacgt tgtttctttt
    47641 tgatttcttc gggcgtcaaa ggctctcttt caaaagagct cagtaccttt tgggaaaagt
    47701 tggagtcagg tcctacagca ggctgctgtt gcttatcttt ttcttgttcc ttattttcat
    47761 ttgaaagctc tgacatgctt actcacctcc gtaataatac attgcttttg gtgattccgc
    47821 tgtccctaga ttctcaagaa cggataagat ctgactagtc tcagaaggct tcaatccctg
    47881 gaattctgct agtttacttc ctaaccatgt actagcaaac ccagatggcg tggaagaata
    47941 ttctactagt tccgcatctt caagctgttg ttcctttcgt atagctttta aagcatcttc
    48001 aggaaatccc aattcatcga ctaaacctac ttcttttgct tggacaccat cataaatcct
    48061 tccatcagca atttttttga cttcttcttc agatttattc cgtccctcgc tgacgattgc
    48121 tacaaaacga ctatatgcgc tgtcgatata tgcttgaagc accgcttctt cttcttttgt
    48181 ctctggtcgc gttcctgaac ccatatcttt caacgctccg cttttaactg ttgtatcttc
    48241 cacacccagt ttttctagaa gtcctgaata attcaatcct gacatgatca caccaattga
    48301 gccggtcact gtttcttctg ttgcaaagat tttatctgct tgagcagaaa tatagtagcc
    48361 accgctagct gccatgtttt tcatgctgac atacattggg atatcatgtt ctttacgaat
    48421 cgtatccaat ttcttagcga tttccgcact ttcgtagaca ccgccaccag gagaatttac
    48481 ttctaaaaag atacctttga ctgttggatc ttcttcaatc gccttcagtt gttccataaa
    48541 atttttatga ttgtaacctt cagaagcaaa taaacctgaa gaccctcccg cagcaatcgt
    48601 tccatctact gataatttta cgattttttg cgtactgtct ccctcttcca acacatttgg
    48661 agctagttct tcatccccat ataaccaact gttcaatccc tcaatttgat cgttttcctg
    48721 aggcttagtc aaactagctg aaactaacga aaccacaaat aatcctgcag caatcagaac
    48781 tgctgcccaa cgtcttttat tcattctttc tcctttctaa tacaagtttt ttatgacgat
    48841 cacagtttct gactgctcag aagtgtcgaa ccgctctgct ttttcttccc gcgcataccc
    48901 ggaaaacgac tcttttgtta attcatcttc taccgtaaac tcacgagctt gagcaacttc
    48961 caaggcatca ggtgcgattt cttggatgct atcccagcca gtatctaaaa atattttcgt
    49021 atcatctgat acaccagctg atgcttcgaa tttggacaat tgttttttta acattcccag
    49081 tgtcatgatc tcaatttttg aatccatttt tactcctcct ctatccgatt actttctatt
    49141 gtaccgaact cctgaaaaag atgatagata aaaacccatt attttttgac ataaaaagga
    49201 catttgacaa agaatttgat atataccaat ttatttttta ttcttttttt gttgacaaga
    49261 acattcgcaa aaggttatga ttttaaatgt ttccagataa aaaacaaaaa ggaggatagt
    49321 tatacctttt aggatagcgc caggtctgtg taaacagatg acgaggagag ggtttatcga
    49381 aagattcggc ggatggccct agggactgca ctctacagaa aatccacaaa aaatgtctgc
    49441 aaagataaaa caaaaggatt ttcctgcagc tggaagcatt catacgaatt cattatagaa
    49501 agtaggatgt tttttatgtg cggaattgtt ggaatgattg gattaaaaaa tgtaacacca
    49561 ggattgattg acggtctaga aaaattagaa taccgtggtt atgattcagc tggtatcttt
    49621 gtctcagatg gcacaactga ttatctggtc aaagcacaag gcagaattca aaatttgaaa
    49681 aacaaaataa atattgatac tacaggaaat ataggtattg gtcatacacg atgggcaact
    49741 cacgggcaac cttctgaaga aaacgcccat ccacatactt cccaaagtgg acgcttcgtg
    49801 ctcgttcaca acggcgtaat cgaaaacttt gaagaactta aaattgctta tttagcaaat
    49861 gaccacttta ttggtgagac cgatactgag attatcgctc atctaattga aactttcgcg
    49921 aaggatacta caacgcaaga ggcgttttta aaagctttgc gggtcatcaa aggttcttac
    49981 gctttcgctt tgattgatcg gacagcacct gatgttattt acgtcgctaa aaataaaagt
    50041 ccactattaa tcggactcgg cgatggattc aatgtgattg ccagcgatgc aatggctatg
    50101 cttgctcata cgaaggaatt cgttgagatt gaagatgaag agatggttac cgtcacatca
    50161 gaaaaagtta ttattcaaaa ttttgcaggt gacatcgtag aacgttcttc attcgaagca
    50221 caagtagatg cttcagatat cgagaaaggt acctatcctt tttatatgct gaaagaaatc
    50281 gacgaacagc ctattatcat gcgtcgtatc gctcaaaaat acgtaacaga agacaatcgt
    50341 gtctctcttg atcaaaagct ggtcgataca ttgtctgata gcgatcgaat ctatatcgtt
    50401 gcttgcggaa ctagttacca tgcggggtta gcaggtaaac agactttgga aaaattgaca
    50461 cagattcctg ttgaggttca tttagccagt gaatttggct ataacacacc tcttctttca
    50521 aaaaaaccat tctttatttt cttgagtcaa agtggcgaaa cagcagacag ccgccaagtg
    50581 ctggtaaaaa tcaaccgtct cggctaccct tcacttacga ttacaaatgt tgctggttct
    50641 actctttcgc gggaagcatc ctttacactg ctattgcatg ctggacccga aatcgcagta
    50701 gcatcaacga aagcttatac tgcacaaata gctgtactaa ctttattagc caaagctatc
    50761 ggtgataaaa aagaatttga aacttctctt gtattcgata tcactcatga attgagttta
    50821 gttgctaatg cgatggaatc ggtcatcgcc caaaaagatt atttagaaga gttggcagaa
    50881 gtatatctca gcgatacaag aaatgccttt tacattggac gtggtgctga ctacaacgtt
    50941 tctcttgaag ctgctttgaa attgaaagaa atcagttata tccaggcaga aggttttgcc
    51001 gctggcgaat tgaaacatgg aacgattgct ttgatcgaag aaggaacacc agtgatcgga
    51061 atcatcagtg aagaagtgac tggtgcgcat actcgaggaa acctgaaaga agtcgaaagc
    51121 cgtggcgcta aaacactagt tattgtctca gagggattag aaaaggaaag tgaccagctg
    51181 gtgctaccaa ctgtccaccc ttatctaact actctaacca cagtcgtacc gactcaatta
    51241 cttgcatatt acgcaacgct attacgggga ttcgacgttg acaaaccacg taatttagcg
    51301 aaatcagtga cagtagaata ataaaagaac aaggaccagt gtcagacatc tgacactggt
    51361 ccttgttctt ttatttttta tgaaaatatc tcttaatcca gtccgatttc ttctttgact
    51421 actgccgcga ttttatcgac atagtaatta actttctcat cagtaggcgc ttctgccatc
    51481 actcgaagta atggttctgt accggaagga cgaactagga ttcttccttc tgatcccatt
    51541 tcagcttctg cctcttcaat cacttttttg attgccggaa catccatcgc accgttttta
    51601 tcacttacgc ggatattcac taatttttgc ggatagattg ttacctctgc tgcaagctct
    51661 gataatttct tacctgtctg tttcatgata ttcagtaact gaattcctga aagcatccca
    51721 tctccagttg tgttaaaatc taagaaaatc atgtgcccag actgctcgcc gccaaagtta
    51781 taatcgtttt tgcgcatttc ttctacaacg taacgatcac caacttgtgt aacgacatct
    51841 ttcaaaccga ttccttccac cgctttgtgg aatcctaagt tactcatgac agtcgtaacg
    51901 atcgtatctt ttttcaatcg atttttttct gccaagtatt tagcacagat aaacatgatt
    51961 ttatcaccgt cgacaatgtt tccaagttca tccaccgcaa taatacgatc gccatcgcca
    52021 tcgaaagcta gtccagcatc tgctcctttt tcaacgacca tctcagctaa acgttctgga
    52081 tgggtagagc ctacgccgtc attgatattt aacccgtctg gatttgtgcc cattgtataa
    52141 aaatctgttt caagatcggc aaacaaacga ttaacacttg ttgaagttgc tccgtttgct
    52201 gcatcgacac aaatggtcaa accggaaagg tcaccgttga tcgtttgtac aaggaattga
    52261 gaatatttca ataagccttc tggaaattct tctacagttc ccaatccttc agctgatgga
    52321 cgaggcaatt catccacttc agcatctaat aatgcttcaa tttctagctc ttggtcatct
    52381 actagtttaa aaccatctgc accgaaaaat ttgattccat tatcttgtgc tggattatga
    52441 gaagcagaaa tcatgacccc tgcgcttgct ttttgcaaac gtgtcagata agcaacacct
    52501 ggtgtagaga tcacacctag ctggaacact tcgataccca ccgataaaag tcctgaaatc
    52561 agcgcttctt ctaacatttg acctgagatt cttgtatccc gccctactaa cacgcgtggg
    52621 cgttttcctt cttcttcatg ctggcttagg acataacctc cgcaccgtcc cagcttaaat
    52681 gcaagttctg gcgtaagttc cttgttggct tcaccgcgta cgccgtctgt tccaaaatat
    52741 ttacccatag ttgtaaaatt actcctttaa cttaattttc tgaggaattt gttctgctca
    52801 tttgtgttga ttcgcttgtt tctgatgttt cttctgtact tgtggtcgag ctgcttgtcc
    52861 cagattccga actggaagaa atggttgttg tcgatggttc cgttcctgaa tcagcagatg
    52921 aaatacttct actcgtactt gtattttctt gtgtccgact gctcgtcgaa gcagagctac
    52981 tgcttgacgt ttgcaacacg ggtgtaatct ccacactaac tgattcgggc ttgatactta
    53041 acccctcttc tactggtatt tcaattttac gagttgttcg atgattgata cctgagacat
    53101 tgatcggtac agcaatactg tcaccgatcg atgcaagcaa atcgctagag ccgcttactt
    53161 ctgcttccag ttttgaaaaa ctgaaatcat agctttctac cccggcagct ggtgttcctt
    53221 gctgtatccc ataaagtcgt accgacttgc ttggcttgat cgaatcagct ctcactgtta
    53281 cttgcactgg atcagagacg atacttaata cttcccctgc tgcattcagc gcctgtattg
    53341 ggacctcact attaatccca tcatctgtga ccatcgaagc gtccacatca gctactactc
    53401 gtttgatttc tgccaatgtt ttgtcacctg tagtgataga gactttttta ggactaacgg
    53461 aagtatcatc aagttcatat ccagatggtg tgaacgttga aggaatcaac gtttccacat
    53521 cgaaatcttt tattaccttt ttctcaaccg ttactgtaat agtcgatggt tcgatctttg
    53581 ctgtgacagc actgctcaaa ttttgaattt ttagcggaac ttcatgtgtt ccttcgccca
    53641 gcttagtgat atctgctgtg acgcggaaca tccgtgtatc ttcgtttgcc tctgcattca
    53701 actgaactcg gtttgcactg gaaagtttga cagtcactgc attttcatat ccatggatat
    53761 agtagcgctt tgaatcataa gaaatcttga tcggcacccc cgtaactgtt tgattatacg
    53821 cttcatttcc agaaagcgta tttggaacgg actggccatt tgcattgaag tacagcgcaa
    53881 tactgaacag caatgcaata aaactgtaaa gaatgcttgt ccgtttctct tttgagatca
    53941 tttgcgatcc cctcccttag tcataccgtc taggaaagat tgaatcacgt ttttcttgtt
    54001 ttttgttttc tcttcagcaa ttagttctct acgcaagatt gccaaatatt cttgctggga
    54061 taagccagcc atcagttcat tatctaacgt gatgctgacg tccccggttt cttcagagac
    54121 aacgatggta atcgcgtcac tgacttcgct tataccgaca gctgcccggt gtcgagtccc
    54181 gaattcttta gggatcaaca tactttctga tagaggaaga taggcgcttg ctactgcgat
    54241 ctttccttct tttacaataa ctgcaccgtc atgcagcggt gtattaggaa taaagatatt
    54301 gatcagcagc tccccagtga tatctgcatc taaagcaatc cctgtctcaa tatactcatc
    54361 taatccagta tgacgttcga tagtgatcaa cgcaccgatt tttcgcttgg acatatattg
    54421 gatggctttg tcaaaagaca agatcatctt ctcatcttcc tgctgttcgc tttttgattg
    54481 tttgaaaagc gaactgcgtc caagatgttc cagtcctctc cggatttccg gctgaaaaat
    54541 cacgatagca gctatcaccc cgtacgtaat gacttggttc atcagccaag acaaggtgtg
    54601 cagaccaatg atttccgcta cgattcggat aacaataaaa atagctacac ctttcagaag
    54661 ctgaatcgct tttgtgcctc gcagcagttg gatcagtttg tatacaagat accagacaac
    54721 cagaatatca agtatgttca ccaaaaaatt cagagagaac aagtcaccgg aaaacatttg
    54781 ctgccaatag tccaaattaa aaagttcccc gatttgaaat gacataaaaa gcctctcttc
    54841 tcttttgaga tttcctctct agtatatcat atgttttaat tttagaggct gatatgctcg
    54901 tatttttctt gagaaaaacg cataattttc aagaaaattg caagtatgcg ttttattttt
    54961 ctctgcaata aatattttta aacttcagag aattattcat tatatttttt ctgttatttt
    55021 tccttttttt cttttgtagc ttgttccatt tttttacgtc gttctgctct ggcagctctt
    55081 ctagcagctc ttctttcttc tgcttcaagg tccgtcgttt tttcttttct agtcttaacg
    55141 ggtacatcga tagattcctc tggttcaagt cgtttcttga ttttttctaa ttctcccgtc
    55201 agtgcagcat agttgtaaaa acgtgtttta gcgtcgtgga cagtcttttc aaataaagct
    55261 tttgcaagat ttggatttgc gttatctagc gaggaaaagc gaacttgttt cctcatgaaa
    55321 tcaggcatag cagagaaatc aggttttttg tagtccaaag tcattgggtt cttgtttttt
    55381 tcgaccattg tagggttata tcgatataat gaccaataac cagaagaaac agcttctttt
    55441 gcttctttaa gcgattcttt cattccgcca agcaagccat gtgtgataca aggcgtatag
    55501 gcaataatca gagaaggacc aggatacttt tctgcttctt caaatgcctt gatcgtttgc
    55561 atttgattgg cacctgaagc aatttgcgcc acataaacat ttccatatgt catcgccatc
    55621 attcctagat ctttctttga agcatacttt ccactagccg aaaatttagc gattgccgaa
    55681 gctggtgttg cttttgaggt ttgaccgcca gtattggaat agacttcgtt atccaaaaca
    55741 aggatattca cgtctgctcc gctggcaagt acatgatcga tcccgccaaa accaatgtcg
    55801 taagcccatc cgtctccacc aatcatccat tggctgtttt ttacgaatag atcctttctc
    55861 ttgtatatat cctccagttc agggaaaatt gatcgttcgc tttcgatcgc agcttgcagt
    55921 tttgctgctc tttgctgcgt tccttcactc tcaaacatgt gtactaacca gtcttccatc
    55981 aattctcgta gttcttctga tacttttggc aatacttctt tgattgaact tgccaaagcc
    56041 tcgcgacgag tctgtgccgc tagaagcata ccataaccaa attcagcatt atcttccaat
    56101 aaagaatttg accatgcagg cccttgacca cattcattgg tcgtataagg agaagcagga
    56161 gaagcaccgc cccaaataga agaacagcct gtcgcattcg cgatcatcat tcgatcaccg
    56221 aacatttgtg tcaataattt tacataaggt gtttcgccac agcctgagca agcgcccgag
    56281 aattcaagaa gcggttgatt gaattgtgta gacaagacag tattctttcc tttgactgga
    56341 tttgcttttt gtcttaatgt cattgcaaaa gcccaattca cagcttgttc tttctgttct
    56401 tcataaggct tcatcactaa agctttttct tttgcgggac aagcctctac acacagacca
    56461 caacctgtac agtcttccac tgaaacttgg atacggtatt ttacgccgtc tgcgcctcgc
    56521 atttctctca cgataaatcc ttctggcgct tcttccattt cctcttcatc tgctaaaaat
    56581 ggacggatgg cagcatgagg acaaacaaag gcacattcat tacacatcgt acagcgatca
    56641 ctaatccact caggtacttc taaagcgacg ccccgctttt ccatagaagc cgttcctaaa
    56701 ggcatttcgc cagttgtcat tccatttgct acaagtgtac caacagagag ttgatttcct
    56761 tcctgtgcat tcactggttc aagtatttct tgtatataac cagtgttcct tttattgatt
    56821 ttttcaggca tctccaacgt tctccaatgg tctgggatcg cgacttgatg aagcagttcg
    56881 gtcgtccgat cgattgcttg tatgtttttt tctacgatag ccattgattt atgtccatag
    56941 cttttcaggg cttcttcttt tagcagcgga agcacctcat caaaaggaat gatgtcagct
    57001 aatttaaaaa aagccgtttc cattgccgta ttgatccggc gtcccaaacc gacttcttgg
    57061 gccaatctca tggcattgat cgtatagaat ctaatgttat tttcagccaa gtaacgtttt
    57121 aattttaagg gcagatgcgt ttctaattgt tcgtcgctcc aaagagtatt caataaaaaa
    57181 atgccacctg gcttcaatcc tttgaccaag tcatagctat gcaggtaagc tggtgtgtga
    57241 caggcaacaa ggtccgcatg ctcgacaaga tacgctgaac gtatcggcgt atctccaaaa
    57301 cgaagatgag agacagtcag accgccagat tttttcgaat cataataaaa atagccttgc
    57361 gcatatttat cggtgtgatc tccaataatc ttgatagctg atttatttgc tccaactgtt
    57421 ccgtcagatc caaatcccca aaacttggcc tggaatgttt gcggttctgt caaatccaga
    57481 ctttcctttg gttccaaaga ttgataagtc acatcatcca cgatccctat ggtaaaccgt
    57541 tttttgcgta tgcttgggtc ttttttcaat tcatcaaaaa ctgctgagat ttggtcggga
    57601 gtcacatctt tagaccccaa tccgtagcga ccaccaataa cagccgggcg aagttcagaa
    57661 tcatataacg cactttgaac gtctaataag agaggttctc cacctgcccc tggttcttta
    57721 cttcgatcaa gaacagcaat tgtttttact gtatgaggca acttttgcac gaatgtttcc
    57781 actggaaaag gacgataaag atggatattc aaaaaacctg ttttccgtcc gtttgcattc
    57841 aagtaatcaa tcgtttgttc aatcgtctgt gcaactgatc ccatagaaac aatgacttct
    57901 tccgcatcgt ctgctccgta ataagtcacc aagtcataag aagttcctcg taatcggttg
    57961 atctctttca tatatttttg aacgatttca ggtaatttat cataataggg gttgatcgtt
    58021 tcccgttgtt gaaaatggat gtctggattc tggttcgtac cactaacagt cggatgattg
    58081 ggattcatcc ctcgtttacg aaatgaagct aatttctcct gattgatcag aggagctaat
    58141 tcttcatatt ccaaaacttc gattttttga atctcgtgac tcgtacggaa tccatcaaag
    58201 aaattcaaaa aagggacttc tccttcaata gaagcaagat gggcaacagg tgctaaatcc
    58261 atcacttctt gtacactgct ttcagctaac atagcaaatc ctgtttgtct tgcagccatc
    58321 acgtctccgt gatctccgaa aatattcaac gcatttgtcg tcactgcccg actagcaacg
    58381 tggaaaacac ttggcagtag ttccccagca atcttgtaca tattcggtat catcaaaagc
    58441 agcccttgtg aagcggtata tgtagttgtc agtgcaccag ttttcaatga tccatggacc
    58501 acaccagctg ctcctgcttc ggattgcatt tctactactt ttactggttg accgaatacg
    58561 ttttcttttc ctgctgctga ccattgatct actaattcag ccatggtcga actgggtgtt
    58621 attggatata ttgcagcaat ctcggtaaat gcataagaaa tatatgcggc tgccgtattg
    58681 ccatccattg ttttcatttt tcgcatgatt catcatcctt gttcatattc tctcttatta
    58741 tctgttccat tataacgtaa aacatgccat ttttttcttt tatttataaa aatattttac
    58801 atgtatctat ttgaattggt catctaattt ttagacaaac agtctaaatc gttgcctttt
    58861 tttgtcagac atgtataaac tataagcgaa agaggtgttt taacatggat atatcagtga
    58921 ttgatgcaac gaaaacaaat acacaaaaag gaatccttta tggttcatcg aatgctccta
    58981 aaaaaatggt cgaattcatc aacttagctt gtccgtattg ccgacaatgg tttgaagaat
    59041 cttatgagct attagaagaa gcagtccaaa gcgggcaatt gcagcgagtg atcaaattgt
    59101 tcgataaaga aaaagaaagc ttacagcgag ggaacgtgat gcaccggtat ctcacaatca
    59161 gcgatggcca aaaagcgatc aaagagatca aacaaatatt cgatactcaa gacgagtgga
    59221 aacatctttc tctccaagaa gtagcggatt ttgcagtaga taaactaaag ctgacagaac
    59281 aaaaagacga acagctgagc caagctgtca tcaacgaagc tgagcaagca catattcgat
    59341 ttgttccaac tgtcatcttg ggtaaagaaa tctttgatga atctatttca atagaggaat
    59401 taaaagaatt gatccaaaca aaataaaaaa ataaggagcg tgggacaaaa gtaaagaaca
    59461 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcttatt
    59521 tcttggagtt aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc
    59581 ttgcatctgc cagattatct catttcttat gacaagctaa gcaaattatc tccaccaaat
    59641 aatttattca tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt
    59701 tctcaatttc ccttaacgag taatatttat gtacggtgat tctcactagt atcccaatca
    59761 ctttttaagt tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt
    59821 tagttgatta gatggtcatt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt
    59881 agcttatacc tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg
    59941 tatttgccac tttgattcgt ataaagctaa caactattaa ctcctctcag ctaatagttg
    60001 ctctcttttt tattcagatt catttttgct gatcacagcc tagtatttca atggttttga
    60061 acactaaacc aaattgttcc attacaacat tcactggctg atgaaatgga ttagtagatt
    60121 tcgattattc aaagcgattt tctattggtg gatcgtgttc ccaactaata agacggattc
    60181 caaacctatc gtacagatct tctaaaccac ttataaattt tgcacctatt tcaggtgttt
    60241 tattgcgtgg atattctatt gttacgccat taaatggata ccaattagga ttaggctctc
    60301 tttttttctg tacatctccc atagaatagt tagcactttc tgggacataa tatattcttc
    60361 gaccatcctc taattcatct aataagatat ttcgtgatcg tctatcactt gctaaatgta
    60421 gacgccacgt gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg
    60481 caacaattgt atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat
    60541 caaactcccc tcgggtaatt tctggtcttg gtggcttagg acccaatatt attctactta
    60601 aaaccgcatt cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt
    60661 ttctatttga taaatttctc attattcgca catcacctct acattctcac atattagttc
    60721 gtaatactcc tttaatatca aaatatctac tggtcgtaat aatattcgct atgaattcct
    60781 actactcgta taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt
    60841 ctatttatcg taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc
    60901 cagttgatta aggtaattcc gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt
    60961 acagttctta tgtatccact ttctcctaat ataaagtcta cctctctttt aaaaccttcc
    61021 tcgagatttc taagggctat atgtggtttt aatgacattg attgtacatt ttcccccata
    61081 taatattgcg cacttcctcg actaaaatag attcctagat cgtccccaaa aggtagatct
    61141 tctctaccac tagctaatct acttctccat cttcgttctc ttttcataga acctgcgctg
    61201 aactcacacc cgttaaactc agctatgaca gtatcttcaa ttatgttaat ctgtccatct
    61261 agttcgtatt caatcctaaa tggaaattct ccataggtga tttccggttc gggtatttta
    61321 ggtgagatta atgcaaagaa aggaaatatt aagcttggca acatcaaaaa aactcctcct
    61381 atgatcacaa ttcccgatgt aatccaaaat ataatttgta caattctcat tgatccattg
    61441 tattccattt tattccccct attactattc atatccgcct atctatatcg atcttccaat
    61501 gcagagataa ttgcttccat accttgattt tttactgagt tacctatgcg agatatcatt
    61561 ctccactaag gtgcatcata ttgtctatct aacatgatta attctccaat aggtgtatac
    61621 tcctccaaaa acaacattta atgcatctcc ctcgacaaca taagctacca tgtttgctgt
    61681 atatgtgttt gagtctaatt cataagcaga taaatttact gtcgctggat taaatataat
    61741 cgcattcgta tttatagcta ctgaatttgc tgcttcagca ccaccttttg aatgtccgat
    61801 cattgtgatt tcaatagtag gatttacgtg gacagccctc accgtttacc ctgaccatta
    61861 tagaaatcgc ttactttttt gaactacgca acaaaggata caatgctcct aacttcactg
    61921 tctccatttt tgtccactaa ggaatataaa aaataggcat taattcattt aattcggtca
    61981 caacacgtta ccttttaaat cattatactg tatctcatac attattatca ataaactatt
    62041 ataaaaatgc aaaaaatgct ttatcaatct attttgtaat tggtcataat atcattgaaa
    62101 tcataccctg tatcgatttt tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg
    62161 caagacagac actggaatgg taattttacc cactattttg ctccaactat ttgtaatcgt
    62221 acccactttt gaaataccga gatgatcatt ttaattgata tgctgattcc caagcaaaac
    62281 cccaagaaat taaatgctct atcgcatcta gcttcttggg ttttgcttat ataaattcca
    62341 acatttaaat tttttatgct gttcggtctc agaaatgccg tttttgctta tatcacaata
    62401 tcaagatgtc aaattattcg tgttgccgat gactttgatt aatttttttc aaattgttta
    62461 ttaagactaa tgctgtcttt ttgcagatac caatgttcga actttttaac attatcatat
    62521 gatcctgcaa gttttaactt cttaccatta gatgttatca aagttataat taaccttgcc
    62581 tttttcccat taggatcttt tattttatga attttctgca ggtaatctct cctaatggca
    62641 gctattttta cctcatcagt gctgataaac cattgatcat ccacatacat actgcctgtt
    62701 atgttgtgtt tttccatttc ttccgagaac gaaaagttta aggctcgttc ttacttccaa
    62761 agttgaatga gtccatggat tggacttagt gtgagaattg agaacaacaa taagaatcca
    62821 tatcctcccg caaacatccc taaggctata ctgtctctcc atccataagt aggattagaa
    62881 gagaaaagag tcaccgtttg taataatatg aaaaggaata atccagcaca aacaccaaag
    62941 ataacaactc caaacattga atttttatac aatgaccatt tatttatagg cttttctttc
    63001 attttacttc cccctactgt tctgttcaca cggaatgaat tcaaagttca taagccctcc
    63061 tctttgaaca tatgaatacg acttgctaac tatactttaa aaaagcttgt tttaagcggt
    63121 tataggcgag ttgtaatctc tattcatctc attagcaaat aacttgccga aaagtctact
    63181 gaaattaaag cagatagtgg aagaattatt tagccaggat agacactatt tgtgaaacac
    63241 atgtctttac ttttgaaatt ctatgatgaa aagccttgga atacagtagc tcttaattgg
    63301 taatactttt tccattataa ataacctctt ttcattttat atttatcccc tctaaaaata
    63361 atatctaatc taatatttta aacttatatt tccctcgctg tgctaataga gcattactaa
    63421 ataaatagaa actagaaaaa agcattacgc ctgccagaat aaaccaatta tttgt
//
LOCUS       DACDKJ010000010        57915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDKJ010000010 DACDKJ010000000
VERSION     DACDKJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.010"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IV328_000723"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IV328_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8936888.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IV328_000724"
     CDS             852..1040
                     /locus_tag="IV328_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8936889.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IV328_000725"
     CDS             1053..1610
                     /locus_tag="IV328_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8936890.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IV328_000726"
     CDS             1642..1884
                     /locus_tag="IV328_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8936891.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2225..3439
                     /locus_tag="IV328_000727"
     CDS             2225..3439
                     /locus_tag="IV328_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8936892.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3470..4897
                     /locus_tag="IV328_000728"
     CDS             3470..4897
                     /locus_tag="IV328_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8936893.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4920..5846
                     /gene="glsA"
                     /locus_tag="IV328_000729"
     CDS             4920..5846
                     /gene="glsA"
                     /locus_tag="IV328_000729"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8936894.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6054..7514
                     /locus_tag="IV328_000730"
     CDS             6054..7514
                     /locus_tag="IV328_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936895.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7735..9498
                     /locus_tag="IV328_000731"
     CDS             7735..9498
                     /locus_tag="IV328_000731"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8936896.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9582..10040
                     /locus_tag="IV328_000732"
     CDS             9582..10040
                     /locus_tag="IV328_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8936897.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10149..10832
                     /locus_tag="IV328_000733"
     CDS             10149..10832
                     /locus_tag="IV328_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8936898.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10979..11593
                     /locus_tag="IV328_000734"
     CDS             10979..11593
                     /locus_tag="IV328_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8936899.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11609..11815
                     /locus_tag="IV328_000735"
     CDS             11609..11815
                     /locus_tag="IV328_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936900.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12354..12911
                     /locus_tag="IV328_000736"
                     /pseudo
     CDS             12354..12911
                     /locus_tag="IV328_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            12969..15008
                     /locus_tag="IV328_000737"
     CDS             12969..15008
                     /locus_tag="IV328_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8936901.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(15117..16268)
                     /locus_tag="IV328_000738"
     CDS             complement(15117..16268)
                     /locus_tag="IV328_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8936902.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(16422..16952)
                     /locus_tag="IV328_000739"
     CDS             complement(16422..16952)
                     /locus_tag="IV328_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936903.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(17051..17401)
                     /locus_tag="IV328_000740"
     CDS             complement(17051..17401)
                     /locus_tag="IV328_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8936904.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="IV328_000741"
     CDS             complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="IV328_000741"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8936905.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="IV328_000742"
     CDS             complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="IV328_000742"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8936906.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(19804..20466)
                     /locus_tag="IV328_000743"
     CDS             complement(19804..20466)
                     /locus_tag="IV328_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8936907.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            20650..22296
                     /locus_tag="IV328_000744"
     CDS             20650..22296
                     /locus_tag="IV328_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8936908.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            22287..23369
                     /gene="uxuA"
                     /locus_tag="IV328_000745"
     CDS             22287..23369
                     /gene="uxuA"
                     /locus_tag="IV328_000745"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8936909.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(23477..25030)
                     /locus_tag="IV328_000746"
                     /pseudo
     CDS             complement(23477..25030)
                     /locus_tag="IV328_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            25178..26319
                     /locus_tag="IV328_000747"
                     /pseudo
     CDS             25178..26319
                     /locus_tag="IV328_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(26445..27524)
                     /locus_tag="IV328_000748"
     CDS             complement(26445..27524)
                     /locus_tag="IV328_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8936910.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(27627..28067)
                     /locus_tag="IV328_000749"
     CDS             complement(27627..28067)
                     /locus_tag="IV328_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8936911.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(28124..28534)
                     /locus_tag="IV328_000750"
     CDS             complement(28124..28534)
                     /locus_tag="IV328_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8936912.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(28714..29148)
                     /locus_tag="IV328_000751"
     CDS             complement(28714..29148)
                     /locus_tag="IV328_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8936913.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(29248..30096)
                     /locus_tag="IV328_000752"
     CDS             complement(29248..30096)
                     /locus_tag="IV328_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8936914.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            30485..32251
                     /locus_tag="IV328_000753"
     CDS             30485..32251
                     /locus_tag="IV328_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8936915.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            32353..33612
                     /locus_tag="IV328_000754"
     CDS             32353..33612
                     /locus_tag="IV328_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8936916.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            33759..35060
                     /locus_tag="IV328_000755"
     CDS             33759..35060
                     /locus_tag="IV328_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8936917.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            35063..35914
                     /locus_tag="IV328_000756"
     CDS             35063..35914
                     /locus_tag="IV328_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8936918.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            35927..36742
                     /locus_tag="IV328_000757"
     CDS             35927..36742
                     /locus_tag="IV328_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8936919.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            36893..38059
                     /locus_tag="IV328_000758"
     CDS             36893..38059
                     /locus_tag="IV328_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8936920.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            38289..38936
                     /locus_tag="IV328_000759"
     CDS             38289..38936
                     /locus_tag="IV328_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8936921.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            39020..39964
                     /locus_tag="IV328_000760"
     CDS             39020..39964
                     /locus_tag="IV328_000760"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8936922.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            40099..41550
                     /locus_tag="IV328_000761"
     CDS             40099..41550
                     /locus_tag="IV328_000761"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8936923.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            41571..42407
                     /locus_tag="IV328_000762"
     CDS             41571..42407
                     /locus_tag="IV328_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8936924.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            42407..43066
                     /locus_tag="IV328_000763"
     CDS             42407..43066
                     /locus_tag="IV328_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8936925.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            43192..43881
                     /locus_tag="IV328_000764"
     CDS             43192..43881
                     /locus_tag="IV328_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936926.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            43887..44843
                     /locus_tag="IV328_000765"
     CDS             43887..44843
                     /locus_tag="IV328_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8936927.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            44887..45399
                     /locus_tag="IV328_000766"
     CDS             44887..45399
                     /locus_tag="IV328_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8936928.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(45655..46203)
                     /locus_tag="IV328_000767"
     CDS             complement(45655..46203)
                     /locus_tag="IV328_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8936929.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(46292..47473)
                     /locus_tag="IV328_000768"
     CDS             complement(46292..47473)
                     /locus_tag="IV328_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8936930.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(47709..49217)
                     /locus_tag="IV328_000769"
     CDS             complement(47709..49217)
                     /locus_tag="IV328_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8936931.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            49376..49567
                     /locus_tag="IV328_000770"
     CDS             49376..49567
                     /locus_tag="IV328_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936932.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            49770..50687
                     /locus_tag="IV328_000771"
     CDS             49770..50687
                     /locus_tag="IV328_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8936933.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            50680..51753
                     /locus_tag="IV328_000772"
     CDS             50680..51753
                     /locus_tag="IV328_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936934.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            51901..52419
                     /locus_tag="IV328_000773"
     CDS             51901..52419
                     /locus_tag="IV328_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936935.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            52400..52792
                     /locus_tag="IV328_000774"
     CDS             52400..52792
                     /locus_tag="IV328_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8936936.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            52868..53803
                     /locus_tag="IV328_000775"
     CDS             52868..53803
                     /locus_tag="IV328_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8936937.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            53936..54715
                     /locus_tag="IV328_000776"
     CDS             53936..54715
                     /locus_tag="IV328_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8936938.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            54708..55025
                     /locus_tag="IV328_000777"
     CDS             54708..55025
                     /locus_tag="IV328_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8936939.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            55269..56879
                     /locus_tag="IV328_000778"
     CDS             55269..56879
                     /locus_tag="IV328_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8936940.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            57036..57884
                     /locus_tag="IV328_000779"
     CDS             57036..57884
                     /locus_tag="IV328_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8936941.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        19269 a        10229 c        11305 g        17112 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
     2101 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
     2161 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
     2221 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
     2281 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
     2341 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
     2401 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
     2461 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
     2521 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
     2581 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
     2641 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
     2701 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
     2761 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
     2821 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
     2881 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     2941 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     3001 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     3061 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     3121 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     3181 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     3241 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     3301 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     3361 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     3421 tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca tgcaacaaag
     3481 caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc
     3541 aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt
     3601 tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat
     3661 ggcaacggtg aaaggatggg aaaaaggcgg tgtctatact tgggtcagtc gcacattggg
     3721 aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt
     3781 gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca
     3841 aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc
     3901 acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat
     3961 cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc
     4021 actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct
     4081 ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc
     4141 aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct
     4201 ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg
     4261 tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc
     4321 aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga
     4381 aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt
     4441 acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc
     4501 aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa
     4561 tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat
     4621 gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca
     4681 ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact
     4741 ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat
     4801 ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta
     4861 cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa
     4921 tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac
     4981 aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga
     5041 tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca
     5101 tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa
     5161 aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata
     5221 tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa
     5281 caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta
     5341 tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat
     5401 caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg
     5461 aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg
     5521 ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg
     5581 gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg
     5641 gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg
     5701 taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg
     5761 cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag
     5821 aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa
     5881 cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt
     5941 ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat
     6001 ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg
     6061 aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat
     6121 tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc
     6181 ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag
     6241 agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt
     6301 ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca
     6361 cgagtacaga actggctgat cttgaattta tttctttgag caaagtatat acaatcaaaa
     6421 aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc
     6481 cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct
     6541 atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga
     6601 ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata cgaggaatag
     6661 agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg
     6721 cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc
     6781 tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata
     6841 ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc
     6901 tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg
     6961 atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag
     7021 atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt
     7081 acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat
     7141 ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag
     7201 cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt
     7261 atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat
     7321 ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt
     7381 gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct
     7441 cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa
     7501 aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg
     7561 tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct
     7621 ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa
     7681 gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt
     7741 acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt
     7801 gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat
     7861 gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat
     7921 gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat
     7981 aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga
     8041 tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac atctacatct
     8101 atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg
     8161 gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat
     8221 ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat
     8281 gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc
     8341 agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat
     8401 ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt
     8461 cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat
     8521 cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa
     8581 acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct
     8641 attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa
     8701 catccagatc attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct
     8761 gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg
     8821 gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc
     8881 ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac
     8941 ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct
     9001 atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat
     9061 ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta
     9121 aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa
     9181 acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc
     9241 acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca
     9301 ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat
     9361 tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta
     9421 gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt
     9481 gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta tactagtatc
     9541 tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag
     9601 aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct
     9661 tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga
     9721 taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa
     9781 aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa
     9841 atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag
     9901 gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg
     9961 cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat
    10021 ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc
    10081 ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac attactggag
    10141 gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt
    10201 tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag
    10261 aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa
    10321 tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta
    10381 ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac
    10441 tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga
    10501 ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag
    10561 cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac
    10621 gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc
    10681 cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag
    10741 gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat
    10801 atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca
    10861 aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact
    10921 tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat
    10981 gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc
    11041 agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat
    11101 cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat
    11161 ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc
    11221 tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct
    11281 gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat
    11341 ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt
    11401 cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata
    11461 tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa
    11521 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
    11581 acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga
    11641 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
    11701 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
    11761 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
    11821 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
    11881 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
    11941 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
    12001 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
    12061 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
    12121 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
    12181 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
    12241 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
    12301 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
    12361 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
    12421 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
    12481 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
    12541 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
    12601 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
    12661 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
    12721 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
    12781 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
    12841 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
    12901 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
    12961 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
    13021 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
    13081 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
    13141 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
    13201 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
    13261 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
    13321 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
    13381 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
    13441 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
    13501 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
    13561 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
    13621 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
    13681 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
    13741 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
    13801 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
    13861 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
    13921 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
    13981 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca
    14041 gtctaggagg aaaagctgca tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
    14101 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
    14161 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
    14221 atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac aacggcacta
    14281 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
    14341 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
    14401 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
    14461 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
    14521 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
    14581 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
    14641 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
    14701 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
    14761 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
    14821 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
    14881 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
    14941 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
    15001 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
    15061 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
    15121 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
    15181 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
    15241 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
    15301 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
    15361 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
    15421 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
    15481 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
    15541 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
    15601 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
    15661 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
    15721 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
    15781 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
    15841 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
    15901 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
    15961 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
    16021 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
    16081 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
    16141 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
    16201 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
    16261 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
    16321 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
    16381 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
    16441 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
    16501 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
    16561 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
    16621 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
    16681 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
    16741 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
    16801 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
    16861 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
    16921 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
    16981 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
    17041 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
    17101 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
    17161 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
    17221 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
    17281 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
    17341 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
    17401 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
    17461 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
    17521 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
    17581 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
    17641 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
    17701 tcactatttc atttcttgga ttcatttcga tcgttctatc tagccaatcc gtattaggga
    17761 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
    17821 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
    17881 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
    17941 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
    18001 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
    18061 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
    18121 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
    18181 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
    18241 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
    18301 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
    18361 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
    18421 ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt tctttttcaa
    18481 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
    18541 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
    18601 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
    18661 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
    18721 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
    18781 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
    18841 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
    18901 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
    18961 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
    19021 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
    19081 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
    19141 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
    19201 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
    19261 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
    19321 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
    19381 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
    19441 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
    19501 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
    19561 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
    19621 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
    19681 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
    19741 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
    19801 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
    19861 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
    19921 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
    19981 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
    20041 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
    20101 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
    20161 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
    20221 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
    20281 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
    20341 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
    20401 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
    20461 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
    20521 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
    20581 catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg ttagtttagg
    20641 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
    20701 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
    20761 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
    20821 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
    20881 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
    20941 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
    21001 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
    21061 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
    21121 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
    21181 attcttgctg tgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
    21241 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
    21301 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
    21361 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
    21421 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
    21481 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
    21541 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
    21601 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
    21661 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
    21721 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
    21781 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
    21841 ccaaatattc ctaatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
    21901 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
    21961 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
    22021 ggaaaaccat tcatcccgag tccagaccca ctattgacag accttcagca taagctttcc
    22081 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
    22141 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    22201 gaaatgttca tcaagatggt agcaggacca aatgccgtac gaaaaacatt acaggattac
    22261 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    22321 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    22381 cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa gaactaaaag
    22441 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    22501 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    22561 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    22621 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    22681 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    22741 aatttggatt gccgggatgg gaacctgagc gcttagctcc tatcaaatcc ttgcttgaag
    22801 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    22861 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    22921 actcgatttt tggattagct cgaatcgtaa agaatcgaga agatttagat tggttgtgtc
    22981 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    23041 cagaaaatga tgtatatggg attttagcag aattttgcca aagagatcga atcccatttg
    23101 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccacataaat
    23161 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    23221 acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    23281 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    23341 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    23401 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    23461 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    23521 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    23581 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    23641 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    23701 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    23761 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    23821 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    23881 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaata
    23941 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    24001 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    24061 caggtataaa caaatcaaat acagtacaaa gggactccac catttctctt gaataaagaa
    24121 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    24181 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    24241 ttggcagatt aggtcgcaaa atatctacag agagatcaat aaaaaagtca atatattcat
    24301 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    24361 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    24421 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    24481 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    24541 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtactatg
    24601 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    24661 gctgactgaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    24721 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    24781 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    24841 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    24901 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    24961 gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta atcgcttctt
    25021 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    25081 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    25141 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    25201 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    25261 gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    25321 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    25381 catcaaagca aaaattgact attcgggaga cttagcacct atactacaat cagctgttac
    25441 atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt tgactatcca
    25501 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    25561 gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa cgccgtatag
    25621 tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc ttgttgaaga
    25681 accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga
    25741 cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga
    25801 gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg taactccaga
    25861 accagatacg gattctggaa accaaacagt accggaaaca aatccagaca cagataatga
    25921 aactgaaaat ccagaaaaac cagaggtaga tccagaagaa aaaccagatg tgactccaga
    25981 accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa aaacaattca
    26041 agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa taaatgaaga
    26101 gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa ccttaaaaag
    26161 tagcaaggat acaacctttg ctgtcctgaa acgaaaaata aacagctacc aaaatccgga
    26221 gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat agcaacaatg
    26281 ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa tgtctgtgaa
    26341 cgcatttttc aaataaaaag aaatatccag aaaatgaccc caaaatacca gattgaaatc
    26401 tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg gatatctgtt
    26461 tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa gaaggtaaag
    26521 atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac aagtaagacc
    26581 acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg attcagcaca
    26641 taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac atctcggatg
    26701 cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac acctgctact
    26761 gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg aaagccaaat
    26821 acagccaagg cgattgtagt gagcaacgta tttaaaagag cgacaacaaa ttgagcttct
    26881 aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc ttgaaagaac
    26941 caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt cttttcaatc
    27001 ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt tcctatatcc
    27061 tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc
    27121 tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa
    27181 aaattaataa cggactggat catgtcaaat gtttgatgaa taagcatcgg gatatacttt
    27241 gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc taaaacacct
    27301 gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag
    27361 ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac aaaaacgaca
    27421 aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg ataaacggtc
    27481 aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg atggaatatg
    27541 aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga gttggttttc
    27601 ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg aaaattatct
    27661 agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag tggaagataa
    27721 gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc ttgagcagtc
    27781 agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat ataatttttt
    27841 tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa atcatttcct
    27901 agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag atactgggta
    27961 taggtaagat tcagcggctt gaggagggga gtgtagtgtc taatgatttc tttagaggcg
    28021 gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt tccacctccg
    28081 ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca ctaactgctt
    28141 tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact ttatgcgctg
    28201 cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct tcaatctcta
    28261 cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca ctgacagtag
    28321 aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta aaacatgcac
    28381 tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag
    28441 ctgcaatatt caattcgaat gtacgatcgg gattatgcac ttctccagag cgtccgcctg
    28501 tgttgatcat tttcgtagaa tacatttttt tcataaagtt accaccttta attatatttt
    28561 atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt ggcataagat
    28621 aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa
    28681 tatcagagac aaaaaataat ctcatcataa acattacata tatttctcaa aaagttgttt
    28741 tctttcttca aaaattactt cacgatggac ctgatgaagc atccataaat aaccaaaagg
    28801 atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt cttgaatctc
    28861 tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga cgatgatatt
    28921 gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag acgtgttttc
    28981 atccagcata tggaatctcg taccatacaa ggtaaaaatg acttcattat tgcctctagg
    29041 gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca gttctagtgc
    29101 tttcaaacta tcatttaata caaaatcgat ttccgttcct ttatgcataa agatctcctc
    29161 tccttttcag tcgaacagtt actagattct tttattatac cagctaaaga aaacgcattc
    29221 aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa atccgcgtcc
    29281 cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt cttctatcaa
    29341 tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct cttttgactt
    29401 tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct cagcacttaa
    29461 gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt tctttcgatt
    29521 gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata aaatagcata
    29581 aagaaactct tctattccaa aaataaacag attaagagaa acaacgaatg cgtctgtaaa
    29641 aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg gagggatcgt
    29701 cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag caaaaatcgc
    29761 acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg gaactattgc
    29821 aatgattaaa ggaaaaacca agcttccgaa aagtacttta aaaaagggtt ttttacctaa
    29881 aaatagaaga gctagtacca acataacgat attcaacact aaaataacta tagctcggtc
    29941 aaagcctaaa gcagcttcca gtaatattcc tagaccgctg acacctccag cagcaatatg
    30001 atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa aaataatctt
    30061 aatgtatgtc catataaatt gtctcgtttc tttcatttat tcacttcctt aattcattaa
    30121 taatactctt atcttattat aacttacttt tcaccttaag ttaactttaa ttaataaaaa
    30181 aattaggcta gtctaaatta ttatgtttca ttcttctaga atatacttct acgccaaaaa
    30241 ctgttaaaaa acttttactt tagaaaattg gagaaattga tcgcttaaga taaattcagg
    30301 acactaaata gtacaggaag tagactaaaa cttttaaaag ttttaagtct atctatttgg
    30361 caagtttaaa aaataaaaat tcggtatcaa aaaactgtta ccgataacat ttttgaaagc
    30421 gttgtctttt gcttctctat cacttatagt gatgacgtca tattaaaaca caaggaggaa
    30481 atcaatggat acagctgcaa tttatcatcg accggaaagt gaatatgcat ttctatatac
    30541 gaatacaaga tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt
    30601 gctatgtgga gatccatata ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat
    30661 gaaaaaatgt ctaagtacca atgttcatga ttattgggaa attgaagtga cgagcgaaac
    30721 aagtcgctta cagtatgcgt tccatgttgt tggggaagat aacacggact gtttttacgg
    30781 tgatcaagga atttttcctt atcgagaaaa tgtgattact gaaccaaatt tttattttcg
    30841 aataccttat tttcatcaaa tcgatcgatt cactattcca gaatgggtaa aagaaacagt
    30901 atggtatcaa atttttcccg aaagatttgc aaacggggac aaagcaaatg accccgaaca
    30961 cactttacct tggggaagta aagatccaga ccgagaagac tttttcgggg gagatttgca
    31021 aggtgtgatg gatcatttgg attacttgac ggaccttggc atcaatggca tctatttttg
    31081 tcccatattt aaagccacat ccaatcataa atacgatacg attgattatt atgaggtgga
    31141 tcccgctttt ggcgataaaa aacttttaaa aaacttgatt gatgaagcac ataaaagagg
    31201 gatacgtatc atgttcgatg ctgtattcaa tcatatgggt gtccattcac cacagtggca
    31261 agacgttctc aaaaacggag aaaaatcgat ctataaagac tggttccaca tccgtttttt
    31321 cccagtagat tcttatcaga tgactgactt accagaaact gctgaaaata tcccatatga
    31381 tacttttgct tttactccat tcatgccgaa actaaataca gcaaatccag aagtgcaaaa
    31441 atatttgctg gatatcgcaa cttattggat aaaagaattc gatattgatg gatggcgtct
    31501 tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag cagttacaga
    31561 aatcaaacca gacatctata tccttggaga gatttggcat tcctctcaag cttggctgca
    31621 aggggatgaa ttccatgcag tgatgaatta tgcatttaca gattcgatta aagattattt
    31681 tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga atgaatcatc agcaaatgct
    31741 ttacagagat caggtgaacg aaggaacatt caacttatta gattctcatg atactgccag
    31801 gatcttgacc ctttgccaag gtaacaaaga attgatgaag tctgtgatgg cctttatgtt
    31861 tcttcaaaaa gggtctcctt gtatctatta cgggacagaa atagggatga ctggtgaaga
    31921 tgacccggat tgccgcaaat gtatgatatg ggagaaggag gaacaagatc tagagctatt
    31981 cggttttata aaagaacttg tttctctaag gaaacagttg tctaagatta tttctgaagg
    32041 ttctactcag tggctaatag taaatgatcg tgaagataag ctctatttca cacggaaact
    32101 agagggacag atcatctatg tttactttaa tcagtcaaaa gaaccttggg tagtagaaca
    32161 ggaaaatgag gtgattctta gtcaaaattg tcagttgtta gaagacggga aagcagagat
    32221 acgacaatac ggatttttga tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg
    32281 tttgcaaatg aagatttgtg cccattataa taagggtgta acagcacgtt gaatacaaag
    32341 gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt aatctcttta
    32401 ggaatcttag gcggattagc ggcttgtggc aatggtaact cttcagcaga aggggaagcc
    32461 gatgaaggaa aaacgttaac agtttccgtt gattctggat ataaagatta tataaacgaa
    32521 ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga aaaggacatg
    32581 ttcgatcagt tggaatcatt agcgttagat gggcctgctg gaaagggacc tgacgttatg
    32641 atggcagcat atgaccggat cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa
    32701 ctgggaaatg aagcagctta cgatgaaaca gacaaagccc aagtgactta tgatggaaaa
    32761 atctacggag aacctgcagt aatcgagacc cttgttttgt actataacaa agatttagtc
    32821 gatacagcac catcaacgtt taaagatttg gagaatctat ccaaagacag tcgttttgct
    32881 tttgagtcag aagcaggaaa aaatacagga tttttagcta aatggactga cttttattat
    32941 tcctatggtt taattgcagg ttacggtggc tatgtatttg gtgatgatgg cacaaatcct
    33001 tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc aacagattgg
    33061 ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc
    33121 cagcaattca tgtccaataa aactgcagca atcattgacg gtccatggca agcgcaaaca
    33181 tacaaagaaa acaatatcaa ttatggtgta gccaaaattc ctactctaaa taatggacag
    33241 ccttatcaac cgtttggcgg aggaaaagga tgggtagtca gcaattatgc taaaaacaaa
    33301 gacctctctc aaaaatggct tgattatgta acgaatcagg aaaatcaaga aaaattcttt
    33361 gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac
    33421 gatgaattaa caacagctgt gattgaacaa tatgaaacag ctcaagcgat gccaaacatc
    33481 ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc agcatcaggt
    33541 tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga agccatagaa
    33601 caaaaatact gattaaaatg ataaccgtcc atttttataa aaaatggaac aggtgcttca
    33661 gataaaagtg gcaagagcga gatggaaaat tatcttgctc ttgttgccat tatcttaaaa
    33721 gcagtttgcg gtttagcaag tttagagggg gcattcagat gcagcaagat aaaacaagca
    33781 atatcaacga aaaaaaaatc acgctttgtt cacttattcc agggatgggg cagtttgtaa
    33841 atggccaagc ttttaaaggt atcgtttttt tcgccatatt cgtcctattc attggtcaga
    33901 tgcttctggg cggatttcat gcattggtag gattggttac tttgggaagt ataccaatgg
    33961 aagatcattc attatttatt ttgattgaag gcactttgca attcatcatt acaatcattt
    34021 tcttgatttt ttacggtttg aatcttttag acgcttatcg agtagcgaag atgcgtcaag
    34081 agggcaaaaa aatcaatcgg acgatgaagg aagtcataca taatataggt gatgaaggtt
    34141 ttccttatct attgacactt cccgcttatc ttctcatgat ttttacgatc attttcccag
    34201 tcctagttac attgttcact gcatttacta attatgactt caatcatatt ccaccagcca
    34261 gtttaattga ttgggtaggg gctaaaaact tttttagtat cttcttttta agctcatatc
    34321 gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt gcaacaactt
    34381 tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca ccagaaattt attaaattca
    34441 aacgtatttt cggtatcatt ttcttattac catgggcggt cccagctttt attactatca
    34501 tgagtttttc gaatatattc aacgatagcg taggtgccat caatactcaa gtgattcctt
    34561 tcataaacca gcttcctttt gtagatatca gtgcaatctc ttggaaaacg gacccgaact
    34621 ggacgaaagt agcaattatc ttagtgcagg gctggttagg atttccctat atctatgtga
    34681 tggttacagg aatcttacaa tcgatttcag aagacttata tgaagcagca aaaatcgacg
    34741 gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag
    34801 cgccaacatt tattacacag tatacgggga atttcaataa tttctccatg atctatctgt
    34861 ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg ttctactgat attcttattt
    34921 cctggatcta taaacttacg actggaactt ctccgcagta ttctatggct tcagcaatca
    34981 cgttgattat ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa aagacaaatg
    35041 cgttcaatat gggggattaa caatgaaaaa atttcattcg agtgctcgat tccaaaaacg
    35101 actttctcac ttttttactt acttttttct tttaatactg tcagtcatta tcatctatcc
    35161 gcttttgatc acagcaagta ctgccttcaa gtcaggaaat attgcagctt ttagtttaga
    35221 tctttcaagt gactggactt taggaaactt tactcggcta ttcagtgaaa cactttacgg
    35281 gacttggtat aaaaatacat tgatcatttc tgtcctaaca atgatcgtac aagtgactgt
    35341 agtaactttg gcgggctata cttatagtcg ctatcgtttt atcggccgca aatcaagttt
    35401 gatgttcttt ttagtgattc agatggtacc gactatggca gcattgactg ccttttatgt
    35461 catggctttc ttattagaag cgctcgataa gtactggttc ttgacaatga tctatatagg
    35521 cggtggaatt ccaatgaata cttggctgat gaaaggatac tttgacactg taccaataga
    35581 tttagatgaa tctgctaaac ttgatggggc agggcatttc aggatttttt tccagattgt
    35641 attaccgctt gtcaaaccga tgctcgccgt tcaagcatta tgggctttta tgggaccttt
    35701 tggggatttc atgttagcta agttcctgct tcgaacacct gaaaatcaga ctgttgctgt
    35761 cggcttacaa acatttattt ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg
    35821 ggcaattttg attgcactac cgatttgtct attattcttc tttttacaaa agaactttgt
    35881 taccggcctg acatctggcg gaacaaaagg ataggagcag agtatcatgc aaacatttcc
    35941 gttaaactat ttttccgcat tacgtacgcc aactcaagtg tttgcgggac gcaaactatt
    36001 atcatggcct aaatttttct tgatatttgt atttttagtt tccttgatgg tcatgcctgt
    36061 gactcttttt tatgccaatc agatccaagc gattccatta gaacaatttc tatccgttca
    36121 ttcgctgatc gatgaacaag gaacccaaaa attttctgaa cttgaattat ctgaaactgg
    36181 gttacaagca tcgcaacaaa cgatagctgt gacccctgaa attttggtag gtgtgtcttt
    36241 atctgagaaa caacaatccg atcatggaac ttttattgat tttgagaagg aacaatgggt
    36301 gatccaacaa aaggataaat ctggtatacg ccgctatacg atgaattata gcccatcttt
    36361 ccaaccagat tctgtacgta cgcccgaaga tttccaacga tttttagaaa gagaatttta
    36421 tgccagtaat cgtccaacaa tcattttatc ttatagttta agtcttggtc ttgtcctatt
    36481 tgtcatgacg agtctcattt tattcggggc ttcctttttc ttgtggatga caagaaaaag
    36541 ccaactttca tccattcata cattcaaaga gagtgcaaat ttgatgctga atgttatggg
    36601 aataggttcc atggcagcag cagttgtagg gtttatccac tttgacttta tcttgatgct
    36661 aagtgtccaa acaacgataa ctgtcttgct gcttttatgg gtttttgtca aaacaagatt
    36721 caaagataag cgtgtcgaat gacaagttta agcgatttat gcgacaatga atgtgaaaaa
    36781 aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc
    36841 ttttcttttt tagtattaac gtaatagggt agttttttta aggaggaaaa tcatgttaga
    36901 gaaaacttta tacaatcaat tattttcacg ttctttctcg ctccctgtcg aagtaaatta
    36961 ttgggacggt acaacgaaac aatacggaga tacggacact cctccccaaa tcaaaatcaa
    37021 tatccatgaa gaaatcccaa tgaaagagat tacaagtaac gcttctttgg cactaggaga
    37081 agcctatatg gatcatcgaa tcgagatcga gggaagtatc caagcattga tcaacgatgc
    37141 ttatcagcaa gcaaacagct ttttacgagg aaaagattat ctaaaatggc tgccaaagaa
    37201 agaaaaacat acgaaacaaa aaaacaaaga agatatccat gcgcattatg acttaggtaa
    37261 cgacttttat aaactttggc tagatccaac tttgacttat tcttgtgctt atttcaatac
    37321 acctgaagat acgttggaac aagcacaaat caataaagtc catcatatct tagacaaact
    37381 ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa cattgatgtt
    37441 cactgctacc aaagagtata atgtcaaggc aactgggatc acactaagtg aagaacaata
    37501 cgatttgatt accaaaagaa taaaagaaga acatttagaa gataaatgcc gtgtattatt
    37561 aatggattac cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg taggtatgtt
    37621 cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag atttattgaa
    37681 accaaaagga acagctttga tccatggaat cagccgacaa caaggcggtg cgacaaatgc
    37741 ttggatcaat aaatatattt tccctggcgg ttatgtccct ggtgttactg aattagttga
    37801 taatattaca aaaaacgatt tgcaactgat tgatttggaa agcttacgtc gtgactacca
    37861 attgacatta gaacattgga caaagaattt tcatcaagtc caagatcaag tcacgaaaga
    37921 aatgggtgat tccttctatc gaatgtggga tctatattta caagcttgtg cagcttcatt
    37981 tgaatcaagt aatatcgatg tgattcaata tttgcttgtt caacctggaa acaatgacat
    38041 cccaatgcat cgtgcataat tgaggcaaaa gtactgttta aagactattt aaaaagaagc
    38101 taagttttat gatgctccag aagtgaactt ttttgtctaa cttctaggat tgtctctaaa
    38161 gcttagtttc tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca
    38221 tgacagaaaa ttttttcttc gttatacttg taatagaata ctattttata acaaagggga
    38281 tcgaatcaat gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg
    38341 cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc
    38401 gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa
    38461 aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg
    38521 aattgttcca agataaatac ccagatactg caattactag tttggaatta gatagtgatt
    38581 ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa
    38641 ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac
    38701 aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg
    38761 aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg
    38821 ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat
    38881 cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat
    38941 ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt
    39001 gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa
    39061 tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc
    39121 tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa
    39181 tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa
    39241 cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca
    39301 aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct
    39361 tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa
    39421 tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga
    39481 agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc
    39541 tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat
    39601 caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc
    39661 aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt
    39721 tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg
    39781 gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac
    39841 cgagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat
    39901 tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc
    39961 atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct
    40021 gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga
    40081 ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa
    40141 gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat
    40201 gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt
    40261 ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa
    40321 gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct
    40381 catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc
    40441 aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga
    40501 aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt
    40561 tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc
    40621 gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca
    40681 tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga
    40741 gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta
    40801 tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt
    40861 gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta
    40921 gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt
    40981 aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg
    41041 attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact
    41101 ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg
    41161 gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca
    41221 acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca
    41281 cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg
    41341 tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca
    41401 gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg
    41461 gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat
    41521 cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa
    41581 aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg
    41641 ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag
    41701 cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt
    41761 atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag
    41821 aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga
    41881 acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat
    41941 attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag
    42001 atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa
    42061 aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg
    42121 tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat
    42181 ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa
    42241 gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa
    42301 aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg
    42361 tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcctct
    42421 aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac
    42481 attagggatt ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc ttttgacagt
    42541 tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt
    42601 tagaggcatt cccgcactag tcctgctgtt tctgttatat tttgggttac cttatcaatt
    42661 aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga
    42721 aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc
    42781 tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc
    42841 agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac
    42901 aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta
    42961 tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa
    43021 agtgcaacag tatttagaaa aacgttctat gcatcagtca atctgaaaaa tagaaatggt
    43081 gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta
    43141 aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc
    43201 ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg
    43261 gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg
    43321 acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat
    43381 tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt
    43441 aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt
    43501 ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca
    43561 gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta
    43621 ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaacagat
    43681 caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga
    43741 tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa
    43801 gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg
    43861 cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg
    43921 acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag
    43981 tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa
    44041 agcaccatcc aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc
    44101 agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa
    44161 cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac
    44221 agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt
    44281 tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg
    44341 actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta
    44401 gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg
    44461 aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt
    44521 atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg
    44581 ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc
    44641 gtcaccatca tcaactcttg attacttcag gaatgattga attattggct ctttaccagc
    44701 aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac
    44761 ttattagtca cctgctagat catccaaaag attggcttaa atattattca aaagttcagg
    44821 aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga
    44881 aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt
    44941 aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa
    45001 ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg
    45061 gaagatcaaa ttgctggaac agctgcatta gtctcaagcg tggatcccgt ttacacaaaa
    45121 atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta
    45181 gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt
    45241 gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg
    45301 gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa
    45361 cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa
    45421 gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag
    45481 aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg
    45541 aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg
    45601 acatagcttt tgtcacgacc tctcggctta tcctacgagt attattcgat acatttatat
    45661 caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac
    45721 taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc
    45781 cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt
    45841 ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg
    45901 ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat
    45961 ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt
    46021 tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt
    46081 ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat
    46141 agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt
    46201 cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa
    46261 gcttttaaaa gtaactttgc ttttttgtat attataccag caagccagtc acaatagcac
    46321 ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat
    46381 ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt
    46441 tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat
    46501 taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga
    46561 tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa
    46621 agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga
    46681 tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga
    46741 aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa
    46801 tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat
    46861 aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca
    46921 tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca
    46981 aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc
    47041 ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg ttgattttac
    47101 ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac
    47161 cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc
    47221 ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat
    47281 gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata
    47341 cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt
    47401 ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta
    47461 ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa
    47521 ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt
    47581 tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag
    47641 tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta
    47701 aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg
    47761 attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct
    47821 tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact
    47881 gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga
    47941 agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg
    48001 tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc
    48061 atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca
    48121 gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg
    48181 actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga
    48241 ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga
    48301 cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct
    48361 acacgattca cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt
    48421 tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact
    48481 ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg
    48541 gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt
    48601 tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag
    48661 gaagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt
    48721 gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact
    48781 ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg
    48841 ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat
    48901 aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca
    48961 taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca
    49021 gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt
    49081 acacgtttcg catgcttgtc gtcaactagc ttcccaccaa attctaaata taatttgtca
    49141 tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca
    49201 aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt catttttttg
    49261 aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca
    49321 acttgtctag atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa
    49381 aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta
    49441 tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga
    49501 aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag
    49561 taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact
    49621 agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc
    49681 cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa
    49741 aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg
    49801 agccagacga actagaggag acgaatgcgg atcaggatac aggacaagaa tcgtcttctt
    49861 tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg
    49921 cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc
    49981 gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga
    50041 tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca
    50101 tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg
    50161 gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga
    50221 aatattccca accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg
    50281 tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac
    50341 aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa
    50401 ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt
    50461 atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag
    50521 cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg
    50581 gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg
    50641 agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca
    50701 gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa
    50761 gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa
    50821 atacacgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct
    50881 tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc
    50941 gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg
    51001 atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa
    51061 ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa
    51121 ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga
    51181 catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta
    51241 ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag
    51301 acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg
    51361 gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta
    51421 gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca
    51481 cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt
    51541 atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa
    51601 gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc
    51661 ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat caattacgaa
    51721 ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca
    51781 ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct
    51841 tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga
    51901 atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg
    51961 atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta
    52021 ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt
    52081 ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg
    52141 agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga
    52201 gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg
    52261 ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg
    52321 cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac
    52381 aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag
    52441 tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga
    52501 ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc
    52561 ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg agtattttga aacgaaattt
    52621 tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa
    52681 aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga
    52741 gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata
    52801 aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga
    52861 aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc
    52921 taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat
    52981 gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg
    53041 tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa
    53101 atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt
    53161 gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt
    53221 tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa
    53281 agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg
    53341 acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt
    53401 ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag
    53461 cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta
    53521 tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt
    53581 tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga
    53641 gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt
    53701 catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata
    53761 cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat
    53821 tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga
    53881 atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa
    53941 caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag
    54001 ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct
    54061 cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc
    54121 tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt
    54181 acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt
    54241 tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt
    54301 ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac
    54361 tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc
    54421 tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa
    54481 aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt
    54541 attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt
    54601 tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga
    54661 tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat
    54721 atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga
    54781 tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac
    54841 cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg
    54901 atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa
    54961 cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc
    55021 tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct
    55081 atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa
    55141 gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt
    55201 ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg
    55261 gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc
    55321 tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc
    55381 aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc
    55441 tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa
    55501 aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg
    55561 tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca
    55621 gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga
    55681 ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc
    55741 cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta
    55801 cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag
    55861 tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa
    55921 aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc
    55981 gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca
    56041 ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc
    56101 cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata
    56161 aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat
    56221 taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt
    56281 cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta gctggaccac
    56341 tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc
    56401 tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc
    56461 accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag
    56521 taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg
    56581 gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag
    56641 caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat
    56701 gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa
    56761 tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg
    56821 gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg
    56881 ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg
    56941 tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt
    57001 ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata
    57061 gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc
    57121 ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt
    57181 atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag
    57241 ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc
    57301 tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg
    57361 gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga cacatggtag
    57421 aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg
    57481 atcgaatccg agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac
    57541 gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga
    57601 tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag
    57661 catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca
    57721 aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc
    57781 agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac
    57841 atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa
    57901 aacataccaa aaata
//
LOCUS       DACDKJ010000011        57375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDKJ010000011 DACDKJ010000000
VERSION     DACDKJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.011"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IV328_000780"
     CDS             591..1280
                     /locus_tag="IV328_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8936942.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IV328_000781"
     CDS             1267..2475
                     /locus_tag="IV328_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8936943.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IV328_000782"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IV328_000782"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8936944.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV328_000783"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV328_000783"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8936945.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IV328_000784"
     CDS             complement(6578..7273)
                     /locus_tag="IV328_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8936946.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV328_000785"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV328_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8936947.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IV328_000786"
     CDS             complement(8472..8663)
                     /locus_tag="IV328_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8936948.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IV328_000787"
     CDS             complement(8684..9574)
                     /locus_tag="IV328_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8936949.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IV328_000788"
     CDS             complement(9561..10328)
                     /locus_tag="IV328_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8936950.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV328_000789"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV328_000789"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8936951.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV328_000790"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV328_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8936952.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV328_000791"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV328_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8936953.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IV328_000792"
     CDS             14944..15282
                     /locus_tag="IV328_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936954.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IV328_000793"
     CDS             complement(15450..16208)
                     /locus_tag="IV328_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8936955.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IV328_000794"
     CDS             complement(16226..17044)
                     /locus_tag="IV328_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8936956.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV328_000795"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV328_000795"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8936957.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV328_000796"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV328_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8936958.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IV328_000797"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IV328_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8936959.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IV328_000798"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IV328_000798"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8936960.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IV328_000799"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IV328_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8936961.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IV328_000800"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IV328_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8936962.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IV328_000801"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IV328_000801"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8936963.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IV328_000802"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IV328_000802"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8936964.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IV328_000803"
     CDS             27086..27385
                     /locus_tag="IV328_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8936965.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IV328_000804"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IV328_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8936966.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IV328_000805"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IV328_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8936967.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IV328_000806"
     CDS             28352..30325
                     /locus_tag="IV328_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8936968.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IV328_000807"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IV328_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8936969.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IV328_000808"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IV328_000808"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8936970.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IV328_000809"
     CDS             33204..34496
                     /locus_tag="IV328_000809"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8936971.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="IV328_000810"
     CDS             35232..36095
                     /locus_tag="IV328_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8936972.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="IV328_000811"
     CDS             complement(36224..37846)
                     /locus_tag="IV328_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8936973.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="IV328_000812"
     CDS             38095..38616
                     /locus_tag="IV328_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936974.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IV328_000813"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IV328_000813"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8936975.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="IV328_000814"
     CDS             40697..41515
                     /locus_tag="IV328_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8936976.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="IV328_000815"
     CDS             complement(41618..41815)
                     /locus_tag="IV328_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936977.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="IV328_000816"
     CDS             41984..42562
                     /locus_tag="IV328_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8936978.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43607..44110)
                     /locus_tag="IV328_000817"
     CDS             complement(43607..44110)
                     /locus_tag="IV328_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8936979.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44469..45494
                     /locus_tag="IV328_000818"
     CDS             44469..45494
                     /locus_tag="IV328_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8936980.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45817..46695
                     /locus_tag="IV328_000819"
     CDS             45817..46695
                     /locus_tag="IV328_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936981.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47171..47287
                     /locus_tag="IV328_000820"
     CDS             47171..47287
                     /locus_tag="IV328_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8936982.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48215..48553
                     /locus_tag="IV328_000821"
     CDS             48215..48553
                     /locus_tag="IV328_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936983.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48647..48877
                     /locus_tag="IV328_000822"
     CDS             48647..48877
                     /locus_tag="IV328_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936984.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49277..49648
                     /locus_tag="IV328_000823"
     CDS             49277..49648
                     /locus_tag="IV328_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8936985.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49922..50614)
                     /locus_tag="IV328_000824"
     CDS             complement(49922..50614)
                     /locus_tag="IV328_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8936986.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50617..51402)
                     /locus_tag="IV328_000825"
     CDS             complement(50617..51402)
                     /locus_tag="IV328_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8936987.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51639..53039
                     /gene="lacG"
                     /locus_tag="IV328_000826"
     CDS             51639..53039
                     /gene="lacG"
                     /locus_tag="IV328_000826"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8936988.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53064..53381
                     /locus_tag="IV328_000827"
     CDS             53064..53381
                     /locus_tag="IV328_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8936989.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53394..55067
                     /locus_tag="IV328_000828"
     CDS             53394..55067
                     /locus_tag="IV328_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8936990.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55081..56712
                     /locus_tag="IV328_000829"
     CDS             55081..56712
                     /locus_tag="IV328_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8936991.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT        17649 a        10569 c        11419 g        17738 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctccgg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
    43321 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
    43381 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
    43441 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
    43501 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
    43561 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
    43621 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
    43681 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
    43741 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
    43801 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
    43861 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
    43921 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
    43981 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
    44041 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
    44101 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
    44161 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
    44221 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
    44281 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
    44341 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
    44401 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
    44461 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
    44521 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
    44581 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
    44641 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
    44701 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
    44761 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
    44821 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
    44881 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
    44941 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
    45001 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
    45061 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
    45121 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
    45181 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
    45241 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
    45301 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
    45361 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
    45421 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
    45481 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
    45541 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
    45601 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
    45661 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
    45721 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
    45781 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
    45841 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
    45901 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
    45961 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
    46021 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
    46081 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
    46141 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
    46201 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
    46261 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
    46321 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
    46381 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
    46441 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
    46501 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
    46561 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
    46621 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
    46681 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
    46741 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    46801 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
    46861 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
    46921 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
    46981 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
    47041 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
    47101 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
    47161 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
    47221 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
    47281 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
    47341 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
    47401 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
    47461 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
    47521 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
    47581 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
    47641 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
    47701 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
    47761 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
    47821 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
    47881 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
    47941 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
    48001 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
    48061 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
    48121 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
    48181 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
    48241 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
    48301 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
    48361 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
    48421 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
    48481 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
    48541 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
    48601 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
    48661 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
    48721 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
    48781 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
    48841 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
    48901 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa
    48961 tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct
    49021 acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta
    49081 gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa
    49141 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
    49201 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
    49261 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
    49321 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
    49381 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
    49441 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
    49501 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
    49561 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
    49621 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
    49681 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
    49741 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
    49801 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
    49861 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
    49921 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
    49981 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
    50041 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
    50101 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
    50161 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
    50221 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
    50281 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
    50341 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
    50401 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
    50461 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
    50521 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
    50581 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
    50641 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
    50701 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
    50761 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
    50821 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
    50881 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
    50941 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
    51001 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
    51061 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
    51121 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
    51181 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
    51241 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
    51301 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
    51361 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
    51421 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
    51481 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
    51541 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
    51601 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
    51661 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
    51721 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
    51781 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
    51841 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
    51901 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
    51961 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
    52021 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
    52081 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
    52141 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
    52201 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
    52261 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
    52321 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
    52381 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
    52441 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
    52501 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
    52561 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
    52621 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
    52681 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
    52741 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
    52801 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
    52861 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
    52921 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
    52981 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
    53041 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
    53101 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
    53161 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
    53221 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
    53281 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
    53341 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
    53401 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
    53461 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
    53521 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
    53581 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
    53641 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
    53701 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
    53761 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
    53821 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
    53881 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
    53941 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
    54001 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
    54061 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
    54121 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
    54181 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
    54241 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
    54301 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
    54361 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
    54421 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
    54481 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
    54541 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
    54601 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
    54661 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
    54721 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
    54781 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
    54841 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
    54901 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
    54961 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
    55021 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
    55081 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
    55141 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
    55201 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
    55261 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
    55321 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
    55381 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
    55441 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
    55501 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
    55561 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
    55621 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
    55681 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
    55741 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
    55801 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
    55861 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
    55921 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
    55981 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
    56041 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
    56101 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
    56161 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
    56221 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
    56281 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
    56341 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
    56401 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
    56461 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
    56521 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
    56581 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
    56641 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
    56701 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
    56761 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
    56821 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
    56881 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
    56941 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
    57001 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
    57061 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
    57121 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
    57181 ccctccatat tatgttggct ctttattccc actctgtttt caacgtaaag tgtataaaac
    57241 agaatgcgat actatcaagg gagttaaaac aatatacaga acagtaaaac actataaata
    57301 aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat catactctgt ttatatatga
    57361 aaactagatt ctgaa
//
LOCUS       DACDKJ010000012        53848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDKJ010000012 DACDKJ010000000
VERSION     DACDKJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.012"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            195..458
                     /locus_tag="IV328_000830"
     CDS             195..458
                     /locus_tag="IV328_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8936992.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            452..802
                     /locus_tag="IV328_000831"
     CDS             452..802
                     /locus_tag="IV328_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8936993.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(826..1440)
                     /locus_tag="IV328_000832"
     CDS             complement(826..1440)
                     /locus_tag="IV328_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8936994.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1754..2176
                     /locus_tag="IV328_000833"
     CDS             1754..2176
                     /locus_tag="IV328_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936995.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2186..2389
                     /locus_tag="IV328_000834"
     CDS             2186..2389
                     /locus_tag="IV328_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936996.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2600..3184
                     /locus_tag="IV328_000835"
     CDS             2600..3184
                     /locus_tag="IV328_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8936997.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            3555..4880
                     /locus_tag="IV328_000836"
     CDS             3555..4880
                     /locus_tag="IV328_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8936998.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            4873..5223
                     /locus_tag="IV328_000837"
     CDS             4873..5223
                     /locus_tag="IV328_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8936999.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            5220..5399
                     /locus_tag="IV328_000838"
     CDS             5220..5399
                     /locus_tag="IV328_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937000.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            5535..5825
                     /locus_tag="IV328_000839"
     CDS             5535..5825
                     /locus_tag="IV328_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8937001.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            6193..6981
                     /locus_tag="IV328_000840"
     CDS             6193..6981
                     /locus_tag="IV328_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8937002.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            6968..7294
                     /locus_tag="IV328_000841"
     CDS             6968..7294
                     /locus_tag="IV328_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937003.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            7643..8683
                     /locus_tag="IV328_000842"
     CDS             7643..8683
                     /locus_tag="IV328_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8937004.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            9519..9851
                     /locus_tag="IV328_000843"
     CDS             9519..9851
                     /locus_tag="IV328_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8937005.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            10442..10771
                     /locus_tag="IV328_000844"
     CDS             10442..10771
                     /locus_tag="IV328_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937006.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            10758..11099
                     /locus_tag="IV328_000845"
     CDS             10758..11099
                     /locus_tag="IV328_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8937007.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            11213..11452
                     /locus_tag="IV328_000846"
     CDS             11213..11452
                     /locus_tag="IV328_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937008.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            11519..11758
                     /locus_tag="IV328_000847"
     CDS             11519..11758
                     /locus_tag="IV328_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937009.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            11844..12515
                     /locus_tag="IV328_000848"
     CDS             11844..12515
                     /locus_tag="IV328_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8937010.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            12568..14544
                     /locus_tag="IV328_000849"
     CDS             12568..14544
                     /locus_tag="IV328_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8937011.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            14557..15213
                     /locus_tag="IV328_000850"
     CDS             14557..15213
                     /locus_tag="IV328_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8937012.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            15309..16136
                     /locus_tag="IV328_000851"
     CDS             15309..16136
                     /locus_tag="IV328_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937013.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            16146..16394
                     /locus_tag="IV328_000852"
     CDS             16146..16394
                     /locus_tag="IV328_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937014.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            16425..18536
                     /locus_tag="IV328_000853"
     CDS             16425..18536
                     /locus_tag="IV328_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8937015.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(18574..20631)
                     /locus_tag="IV328_000854"
     CDS             complement(18574..20631)
                     /locus_tag="IV328_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8937016.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(20745..20957)
                     /locus_tag="IV328_000855"
     CDS             complement(20745..20957)
                     /locus_tag="IV328_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937017.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            21144..21611
                     /locus_tag="IV328_000856"
     CDS             21144..21611
                     /locus_tag="IV328_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937018.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            21608..21850
                     /locus_tag="IV328_000857"
     CDS             21608..21850
                     /locus_tag="IV328_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937019.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            21868..22170
                     /locus_tag="IV328_000858"
     CDS             21868..22170
                     /locus_tag="IV328_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937020.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            22241..24295
                     /locus_tag="IV328_000859"
     CDS             22241..24295
                     /locus_tag="IV328_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937021.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            24295..26127
                     /locus_tag="IV328_000860"
     CDS             24295..26127
                     /locus_tag="IV328_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8937022.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            26822..28738
                     /gene="ltrA"
                     /locus_tag="IV328_000861"
     CDS             26822..28738
                     /gene="ltrA"
                     /locus_tag="IV328_000861"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8937023.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            28855..29655
                     /locus_tag="IV328_000862"
                     /pseudo
     CDS             28855..29655
                     /locus_tag="IV328_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            29652..32183
                     /locus_tag="IV328_000863"
     CDS             29652..32183
                     /locus_tag="IV328_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937024.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            32233..32565
                     /locus_tag="IV328_000864"
     CDS             32233..32565
                     /locus_tag="IV328_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937025.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            32566..33159
                     /locus_tag="IV328_000865"
     CDS             32566..33159
                     /locus_tag="IV328_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937026.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            33322..35016
                     /locus_tag="IV328_000866"
     CDS             33322..35016
                     /locus_tag="IV328_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8937027.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            35704..37581
                     /gene="ltrA"
                     /locus_tag="IV328_000867"
     CDS             35704..37581
                     /gene="ltrA"
                     /locus_tag="IV328_000867"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8937028.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            37712..38089
                     /locus_tag="IV328_000868"
                     /pseudo
     CDS             37712..38089
                     /locus_tag="IV328_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            38089..39294
                     /locus_tag="IV328_000869"
     CDS             38089..39294
                     /locus_tag="IV328_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8937029.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            39310..39954
                     /locus_tag="IV328_000870"
     CDS             39310..39954
                     /locus_tag="IV328_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937030.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            39965..40771
                     /locus_tag="IV328_000871"
     CDS             39965..40771
                     /locus_tag="IV328_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937031.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            40793..41023
                     /locus_tag="IV328_000872"
     CDS             40793..41023
                     /locus_tag="IV328_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937032.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            41569..41844
                     /locus_tag="IV328_000873"
     CDS             41569..41844
                     /locus_tag="IV328_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937033.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            41886..42308
                     /locus_tag="IV328_000874"
     CDS             41886..42308
                     /locus_tag="IV328_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937034.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            42327..43919
                     /locus_tag="IV328_000875"
     CDS             42327..43919
                     /locus_tag="IV328_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937035.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            43931..44266
                     /locus_tag="IV328_000876"
     CDS             43931..44266
                     /locus_tag="IV328_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937036.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            44413..44607
                     /locus_tag="IV328_000877"
     CDS             44413..44607
                     /locus_tag="IV328_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937037.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            44639..47011
                     /locus_tag="IV328_000878"
     CDS             44639..47011
                     /locus_tag="IV328_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937038.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            47072..48532
                     /locus_tag="IV328_000879"
     CDS             47072..48532
                     /locus_tag="IV328_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8937039.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            48543..50747
                     /gene="topB"
                     /locus_tag="IV328_000880"
     CDS             48543..50747
                     /gene="topB"
                     /locus_tag="IV328_000880"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8937040.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            50993..51586
                     /locus_tag="IV328_000881"
     CDS             50993..51586
                     /locus_tag="IV328_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8937041.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            51602..52501
                     /locus_tag="IV328_000882"
     CDS             51602..52501
                     /locus_tag="IV328_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8937042.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            52504..52989
                     /gene="ssb"
                     /locus_tag="IV328_000883"
     CDS             52504..52989
                     /gene="ssb"
                     /locus_tag="IV328_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8937043.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            53355..53615
                     /locus_tag="IV328_000884"
     CDS             53355..53615
                     /locus_tag="IV328_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937044.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT        19808 a         8402 c        10798 g        14840 t
ORIGIN      
        1 tttttcttac ctgattctaa agattttgtt ttcttaaaca tattatgatt tcctttcgac
       61 tattttcata taaatgttaa catagtttat ggattcatgg aatgttaatc taatttaata
      121 gaaaaggata attaagtata tacgtttttt cttatgtatg ttatactatg tatatacaga
      181 aagaggtgtc tactatggaa atgttaccta taaaaaaatg gggaaatagt aatggcctta
      241 gattgccaaa gtatattatg gaatatcttg gaattcatac agaagacaaa gtgaagataa
      301 tacaagaaga agtaaacggt cagaagcggc taattatcga agcggctaat atagaaaatg
      361 aattaacaat tgaacagtta tttgaaaatt acactgatga aagaaatcat gtgacgattc
      421 aaaatttagg tgaagcagta ggtaatgaaa aatggtgaga aaacctagac aaggcgatat
      481 tctattattg aatacagcac ctcgatctgg acacgaacag acaggtaaaa gaccttatat
      541 tgttttaagt catgatatta ttgcggatta tagtaacgtt gttacagttg ctccaatttc
      601 tagtacaact agaaactatc cgctatatgt aaatataaac tcagaatatg aaatgaagac
      661 aacaggaaaa gtattattag atcaactaac gacaattgat tatgaagcca gagagtgtat
      721 ttttttggaa aaagcgcatg atagtttagt tgaggagtta ttgatgaaag ttagaacagt
      781 ttttcaaaaa gtaagcaaat aaaatagaaa cagcagcatg atcaactatt cgtgattgac
      841 catgctgtct ttttttattc tatatacggt ttgtctcgta acattgtagt cttttgcaat
      901 cttagcgatc gcaacacctt tttttagatc ctcaacgatg tttttataaa caagacgacg
      961 ttggggatct tttgcattgg cactgtatag cttaggacgt cctttataaa caccgttcgc
     1021 ttttgcaatc ttaatgcctt gtgcctgacg tcgtttagat tcggtacgtt cttgttcagc
     1081 gatcatggcc aaaatttgaa tgatcaaatc tttaatgaat ttatccagga gaggattgcc
     1141 aattgcttcg gccatgatcg gtagacttgt gatgatcagc tgcacatttt tcttcttcaa
     1201 gtaattgact gaagcgatga tctcgtcgta atttcttcca agtcgatcaa tagcttccac
     1261 aataaaaata tcttgatctc ggacgaaatc cagtgcttct tgaaaaacag gtcgatgagt
     1321 aatcgttgcg ccagattgtt tctcaatgaa aattctctct gcaccgaatt ttttcatttc
     1381 ttcaatctgc cgttgttcat tttgatcgat ggatgagaca cgtatgtatg caactctcaa
     1441 aatttttacc tactttctgc aactatttaa aaaacaccct aatcatacac gagaaaacca
     1501 gatttttcaa tgtcgcggtc gtgtctagtt agggtatacc ttatatagac aaaaactaat
     1561 gatggaggaa atagtatgtg tcacttttct ttttaaatgg caatattttt tgtaaataaa
     1621 tgtcaatcaa gaaaatttaa acgattatga cattaaaaat atattttatg aattttctag
     1681 tatataactt gatttatctg ttaagattta ttaagaaaac gttttcttat ttttgatttc
     1741 gaggtgatag acaatgaaaa ataagtttat tttatcttta ggtattttaa ctattggaat
     1801 tagtgtaact ggaactacaa ttcctacatt tgctgatagt acaaatgaac caactactgt
     1861 agtagataat aaccaagaat taactccttt agataggaac ggaatagcca ttggtaaccc
     1921 aactaacgac ttaactgtta ctgaaaatta tttaatagtt tctccattac ttgctcgagc
     1981 agcttctaaa acgatacgag tgacaggaac gatgattttt gataaagttg gttggccttc
     2041 tagacctcct caatcacgga attggaaaga aaaaagaaat ggttattggt actatggaaa
     2101 attgtattta caaagttata aatcagctgg aaacaattgg attgcgactt attcaggaac
     2161 cttaactaga aagtagggat tttttatgcc agaatttgtg aacacaccaa atttttatca
     2221 agatagcgat attctaagta aaattaattt accgaaagaa gaatcagctt tacaagttca
     2281 gcgaacttta caggagacac aaaaaattca aaaatcaatt tctaatctag aagattctga
     2341 agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat gattttaaaa
     2401 aaagagtgtc tagtcgacat tctttttttc taattgaaag tgttcataaa atcacccatt
     2461 tctggacact tccttttttt gctttatttt tcaaaaaaac aattcatttc atgttcataa
     2521 acccataaca atatctatgt tcagttaaac tatatggata aagttatgtt acaataaaat
     2581 taatgaagga gtgatttttt tgaaaatagg atacgcccgg gtatctactg gtttacaaaa
     2641 tttggattta caagaagaca gcctaaataa atttggttgt gaaaaaattt ttactgatca
     2701 tatgagtggg gcaaaaagta atcgaccagg attagaaatg gccattgatt ttgttcgctc
     2761 tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg aagatttgat
     2821 ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt catagtattc aagaaaatat
     2881 tacgatggac aaatcaagtt ctacaggaca gctcatgttt catttgtttg cggcatttgc
     2941 agaatttgag cgtaatttga tcctggaacg atccgcagca ggacgagcag ctgctcgtgc
     3001 aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa caagatttgg aattactgaa
     3061 gactttagtg gatagcggta cacctattaa gactattgcg gaacggtgga acgtctcaag
     3121 aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga caaattctca
     3181 ttagtttttg tcatttaatg ctgaaaattt ttgccattta tctagaaaag tgacaatagt
     3241 atgagaattt tttgtaagtg tctactgata acttattatc aaatgttgta aggattttat
     3301 tcatttgtta atattaaaga acggaactcc aaaaaagtcc gtactctaat ttgcagtctc
     3361 tcactctttt agagtgagag tttttattag attttcaaat taattagtaa agtgatagaa
     3421 taaattgatc atacaaatca taagaagaag cagggtgata gcatacctat gcttcttttt
     3481 ttatgtattt agctatttct tctttacaac atcgaacata tgtttgtata atgtggaaga
     3541 atggagtgtt atttatgatg gattattcaa aagagccagt aaatgattac tttttaatcg
     3601 atatgaaaag cttttacgca agtgtcgaat gtatagaaag aaatttagat ccattaacaa
     3661 cagaacttgt tgttatgagt cgatctgata atactggttc aggattgata ttagcttctt
     3721 ctcctgaagc aaaaaagcgg tatggtatta caaatgtgag tagaccacgt gacttaccac
     3781 aaccatttcc taaaacacta cacgttgttc caccacgtat gaacctatat atcaagcgaa
     3841 atatgcaggt aaataatatt ttcagaagat atgtggctga tgaagatcta ctgatttact
     3901 cgatcgatga atcgatttta aaagtgacaa aatcactgaa tctttttacg actgaagaaa
     3961 cacgaagtca gcgaaggaag aagctcgctc aaatgatcca agaacgtatt aaagaagagt
     4021 taggtttgat tgctacggta ggcgtcggcg ataatcccct tttggctaaa ttagccttag
     4081 acaatgaagc aaagcataat gaaggattta ttgcggaatg gacgtacgaa aacgttcctg
     4141 agaaagtatg gaatattccc gaaatgacgg acttttgggg aattggttct cgaatgaaaa
     4201 aaaggctcaa ccaaatggga attttgagca ttagagacct tgctaactgg aatccttata
     4261 cgattaagaa tagattaggt gtgattggat tacagctgta cttccatgct aacggaatcg
     4321 atcgaacaga tatagcaatc ccgccagaac cgacaaagga aaaatcttat ggtaattcac
     4381 aagtgttacc acgagattat actagaagaa atgaaatcga gctggtggta aaagaaatgg
     4441 cggaacaagt agccattcgt atcaggcagc ataattgtaa aacaggatgt gttcatttaa
     4501 acattggtac gtcgatctta gaaacgagac caggattttc acaccagatg aaaataccta
     4561 tcacagataa tacaaaggaa ttgcagaatt attgtctttt cttgtttgat aagtattatg
     4621 aaggacaaga agtaaggcac gttggaataa cctatagcaa gcttgtgtac accgattcgc
     4681 ttcagctgga tttatttagt gatccacaaa agcaaattaa cgaagaaaat ttagataaaa
     4741 ttattgacaa gattcggcaa aaatatggtt ttacgtcgat tgtacatgct tcttcaatgt
     4801 tagaaagcgc tagaagcatt actagaagca cacttgttgg tggtcatgcg ggcggaaatg
     4861 ggggaattaa aaatgattga ttacaatgat cgtggacgtt taaagtgggc agggttttat
     4921 ttatctgatc atactgaaga aattgatgcc gacaatgaac aacgaaccag tcaaaattta
     4981 gcaaaagaac aaatgacgac tgaagagatt gcggaagtat tgaacgctgc agtcataaaa
     5041 aatagatcag tatctattca aaaagaagaa cgaaacgctg aaggatacta tccagaagat
     5101 attgttggca agatcttagg tagtgatgag ctggggatct acgttggaaa agacaaaatt
     5161 cactacgacg aaatacgtca tatcgaattt taccaggata ataaatggag tcgattagaa
     5221 tgaacattat ccagcaatat gagctaaagt atataacctt tgatcagcta tcagaagaga
     5281 tttggggtta tggccaacgt ttgataaatg aggttggtgt tgagcgcttc tctttctatg
     5341 tagaagctgc agcaggttat cataatttta ggttttatat tttcccgtta tatatataag
     5401 gtattttacg gattctatag agttatctct atgaatcctt ttttgttttt acaaatagga
     5461 gtagatacta gaataatgcg tgtgttaaac tattattgca aacgcaataa taaaagaaag
     5521 taggcttacg caccatgtct atcaatacat ttgtttactc gcacccgatc aatgtttaca
     5581 ttatcaagaa tttaggaatc actgtagaac aattttgtga gttatatgcc tacccacaag
     5641 gaacggttgc aagctggata acccgacaac gtagaataaa atcattacct gcttcattcg
     5701 tttatgattt aagcctggcc tctagtttaa acatgtctga tgtttatgaa aagctcctgt
     5761 ctttagaaaa agaatatgat tccttcaaga ttaaacatga taaaaaaata aaaaaacata
     5821 tataatatca atcacaattg tataatatta cgatatctta atatcatgat atcgtaatat
     5881 tgtttttttc taagaaaatg cactataaga agggaattat tagtaacaat atagacagtt
     5941 taataatgtt taaattattc tagttgctgc tcgcaaaaca tatttcttat gattaataaa
     6001 aaattttaaa gtaataaaaa gtgattattt tactttcgaa taacatatga ataatcttta
     6061 tatccgaacc gatatatatc tttaaaacaa aataatcaca caatattaca atataatgca
     6121 tatacgatat tgcaatatca caatattatg atataattat attataatat aagatgaatg
     6181 ggggatattt taatggcaag aatattgtca gtagccaatc aaaaaggtgg agtttctaaa
     6241 actacgacta gcgttaattt agcagcatgt ttgaaatttg tttacggtaa aaatgtactt
     6301 ttagtggaca ttgatcctca gggaaatgct acagataatg taggtttcga tatagatgga
     6361 acaaaccaac ctacaattta tgaagtatta aaaggggagg ctgacattac agatgctatt
     6421 ctggactata aaggaattga tgttttgcca gccgacatag ccctttcttc tgcagagagg
     6481 gaatttactc aagtaggaag tgaacacagg cttaaaagag tacttcaacc aatcaaggac
     6541 aactatgatt atattataat tgattgtcca ccaagtttag gaatgcttac agtcaacgca
     6601 tttactgttt ctgatgaaat tatcattcca gttgaagctg cgtatttctc attaaaagga
     6661 ctaataaagt taagtgaaac tattgaaacg gtaaaagaat atacgaacga caagcttgaa
     6721 gtacgaggag tcctatttac taagtataat aagcgttata acatttctaa agaaatgacg
     6781 actgcagcaa ccgaaatttc tagagtcatt ggtgcaaaaa ttttcaaaac ttatattcgt
     6841 agaacaataa ccgttgacga agcgcaagct gctggatcag atctaatcaa ttttaataaa
     6901 ccctcaacag ctgaagatga ctataaagca tttacagaag aatatttaaa agaagttggt
     6961 gattttgatg gcagaagata atcgcagtca agctgcagta agtagactac taggaaataa
     7021 aaataaaaag aactacaaac caaaagaagt agaagaaatc gaattaacca agaaaaatat
     7081 aagtaaagtc ataaaagatc aaggaagtag tgtaagaaca gcagatagca ttcgtataaa
     7141 tccaattcta atgtcagcac tcaaatattg gacgactatt gctgaatcag acaaaagtaa
     7201 accagacgtt atagaagatg cgctattaaa atccatacct gaagaatact tgattgaagg
     7261 atataaacta gcaaagaaac aaaataaaat ttaatttaca ttcaatatca cgatatcata
     7321 atatcgtgat atttttttta taaaaatcag tagtatcaaa gctgataagt cgcttatctt
     7381 catattgtaa tattacgata tcgtgatata atgttattgt aatatcgtga tatagttacc
     7441 atgagcttaa accctgaaat agagcggttt gctttaattt attttttaag gtaaactaaa
     7501 aaaagataaa aaaagactac tgctttaaca aagtttggcg acttatcagc agtagaaaaa
     7561 tatatagtgg atagatatat ttgtttaatt cattgtaaca tagttttgaa caaaacgcta
     7621 tcctacagaa agaggattgg ctatgaatga ttttaattat tacaaatcaa aagaaattta
     7681 tcgtgaaaaa tattatcaaa tgcctaaggt attttttact aacgaaaaat acatggatct
     7741 ttcaaatgat gcaaaaattg cttatatgct acttaaagat cgatttgatt attcagtcaa
     7801 aaataactgg gtagattctg atgataatat attcttcata ttcacagttg aagaattaat
     7861 gaaattgtta caatgccgcg aaggtaaagt atctaagatc aaaaaagaat tagaaaaagc
     7921 aggtcttctg aaacagaaga gaggaagagt aaataaacgt gatggtaaga ttgaatctat
     7981 gcctaattta ttgtacttag gcaagccaga agttacgaat caagacgtct ttaaaatcat
     8041 ggaagaggaa gacaatactg attccaccgt aattgcgaaa atcgcaaata cggcaaaacc
     8101 gcgaaaaatc aatgattcta ccgtaattgc gaaaatcgca aatacggcaa aatccagtca
     8161 tatcaaggac tcaaccgtaa ttgcgaaaat cgcagacaat ctattctatt ctaattctat
     8221 agacactaat agacacataa tagacactga aactgaccag ttacaaaatc aagttttgct
     8281 agataacttc gtcgacatca tgcaagagga cagtataaat acatttgtac cagaaaatgt
     8341 attaaacttg ataaaaacat tttcttcaag ttacagtgaa gctcaacaaa ccgtccaaac
     8401 tattcataat gcaaagaaaa aagctgaaga attagagcaa actattgtcg tttatgaaga
     8461 actggaatct tatggaattg atgctgacaa aggattatat atgactttac tgaaagctta
     8521 tcaaaaacaa aaaactgaaa aagtaaacaa tttgcagaat ctaattttcg tttatgtaaa
     8581 aaattggttt gtcgaaaaag ctattgcggc aaagcttgca aacgaacaaa gagaaacatt
     8641 taacgaatta ccaacagtta gtacggaaaa ttggttagag tagttgataa aaagcaatta
     8701 ctataaaaat gatcaatttt aaattagaac ttttttatga ttttaatttt attacaaata
     8761 ttacaaataa atagttagta ttccggagtt tttagattaa aagtttatga aaacaatcac
     8821 aatcgtaggt tttaaattca taatcgttag atattttagt tatttttcac tcaaaaaaat
     8881 agggaactag tgtttgcata tgtgcttttt tgagttataa taattcatga agagaggtaa
     8941 ttcacttatt tccccaagct gaaaagcctg atattacttc ttttcaggca gtgctgctaa
     9001 tattcaatta cacttttttg tatattttta gttgttttaa atttaattgt ctgctatgct
     9061 tgtgatgaat tgattttcgt tctgcacttt tggacgataa atttggacaa ttgaaaatac
     9121 aaaaaaaaga aaggccctgt gagactcgga aggctggtaa ccttcgtcga cgtccacagg
     9181 tggcattaag atcactccct taatgcgttt acctttcagt gttacggtat tacccgtaac
     9241 ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg ttaaggttaa
     9301 aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa tgtccgtgtg
     9361 gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga tgcgttccct
     9421 cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac
     9481 ttgttaaaaa tatttaaagt aagtgggtgg attattttat gtcatataca ggaattttga
     9541 gtctcgaaga tatttgtcac tacggtaaac ggtgtaccgc tacagaaaag atcacgaaga
     9601 aactatcaac tggtcaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag
     9661 acaaagtctc agaagaaatg atttattaca ttgggaaaca aaaacaaata attttaaaag
     9721 atcctatccc tttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg
     9781 tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt
     9841 taatcagctg aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa
     9901 atattgtcga gacttctaaa ggaacttaga atcactagaa aaataaggag tagacttgat
     9961 ctatcaacaa ttcctttcgg ggggtgacgg ggtaggtttt tacgattggt aacgtaaaaa
    10021 attggtcaat cgagtaaatg cccttgtttg gatagtggtg aaatgaattt gtgtaaataa
    10081 tatgcacaga gtaaacaaga gggaatttag aagttagaca agttagaata gcgcctagat
    10141 taatcatgag cagctactaa gggatctagg ctttgacgca aactcatgga ataaggagta
    10201 ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag
    10261 atacttgcga agacgttgat ctttggatca atcgagcatt taccgttttc tactagggtt
    10321 tctcgtttcg gacgccataa acgagtcgat ttattttata tatttttgag tgaacgaaaa
    10381 acgataaaaa tattcgttat gaagtaaaac taatttagta gatagcaaag gtggtagagg
    10441 aatgattcaa aaatttgcga aaatgagtaa aactgcatgg attgaaaagc ttgaaaatga
    10501 cttgagtgtc atgcgattag ctccaaagaa attcaaacga gagattgcag aaaaagaagc
    10561 aattttgtcg gtattgaatg cggtagacga taaatgtcca gtaaaaatta gtttttcaga
    10621 tatagcaaca attatcgaag gtatttatta tggtgaagtt gatgaagaga tatcttctac
    10681 aaaagattta ttagattttt tggataaaaa caaagatacc tttatagtac taactgatta
    10741 tgaggaggtt acaacaaatg aaaaattatg atccgaatag ccgatgggga acacatacga
    10801 ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga agaggcggaa
    10861 attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat caaaaattga
    10921 ctgataatcc aattggattc ggcttgctac ctgaagatga tcaaggtaat gaatggttta
    10981 aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact tggtcatatt
    11041 tgagtgatta tattgtcagc gttgaaatca ttgaatttgt agcggataaa gaagaataaa
    11101 aactggagga ataaatttta cagataaagg cttagaagga gtcacgacga ttaataaaaa
    11161 ggtagtatta ccttaggcat tgagtaagga atttatttag gaggaaaatt agatggcgcg
    11221 accagtttta gaggcgatgg atatggatac tttgacaaaa ggaaggtatg tacagctgtt
    11281 agctcgtggt tattcaccta aagaagtatt caaatccctg tctaaaggaa cagatatgga
    11341 aaaagaaaga ttacgaaaag aattcgatta ttggagaacc cataatggaa tcaaggattt
    11401 gaaaccagca cggccagtaa ttaaacgaaa gaaaaagata acccaagact gaagtgtaga
    11461 gcgtatgtga tcataggctt ttttattttt gcaaaaaaca aggaaggtag gaaaactcat
    11521 gaattatgaa gaaatcgaaa acagaaaaaa agtgtcgaaa gaaatggaag aaaagttgct
    11581 aaaaacgatg aaacaaaaac accttaaaag attatctgtc atgcaatata tcaatgatat
    11641 gcagataaca ggaaaagaga aagcttgttt gttaggttca atgaaaaact ttgaacagtt
    11701 gagacgaact tatgtgaaaa aaagttctaa ttgtcaacta ttgttagaag tctcatgaca
    11761 ttcattaata gaattcattt ttaaaacttt ttctatggaa atgagaaaaa gattttttgt
    11821 tttgtctgaa agggggtagc tggatgagaa aatggttatt tggtttttta ggagtcgctt
    11881 taatcgtctt ttgcagtgtg tttggctatg ttagctacca aaaacatgaa ggaaaggtgt
    11941 ttaagcaaaa tatagaaaaa aagatgcccg tagatcaaat caatgaacat gcaaagagtt
    12001 ataaggagga tgcaacgaac gtcaataatg atatgtcttt ggggcaaatg ttgtccatac
    12061 aaaaagaagc aatcgaaatg ggtgtcaata aacaagcctt tgcacaaatt cagatacctg
    12121 cacttggttt agcattgcca atttttaaag gggcaaatca atacactttg agtcttggag
    12181 cggctactta tttttatgaa gatgccgaaa tgggcaaagg aaactatgtc ctagcaggtc
    12241 ataacatgga aatgccaggt gttttatttt ctgatattca aaagttgtca ttaggggagg
    12301 tgatggactt agttagtaac gatggcgtct atcgttacaa ggtaacgcgt aaatttatcg
    12361 tacctgagta ctttaaatta atcgatggcg ttcctgaaga gaactctttc ttgtctttac
    12421 caaagaaagg agagaaaccc ttattaacgt tgtttacttg tgtttatacg tcacaaggaa
    12481 aagaaagata cgtggtacag ggagagctac aataacaagg ttttaatcaa cgcgagtcgt
    12541 tgaaatatga aatgaggaga gtaaaaaatg gcaataaatc ggtatgtaaa aaaagctgga
    12601 atgcttgttg gaatgttagg aatcattgca agctgctttc aggggtatca agtacacgca
    12661 gaagatgtca cgcaaaaaac accaccagag aaggtcaata tcacggttca taaattaatg
    12721 tacgatcaag gaacgcaatt gaatgttgat attgacggga tcaaaaacga tggttatacg
    12781 cacgatcaat atcctacagg tgtaacgaaa tacaacaagg cagattatgg tgatgttgag
    12841 tttacgttag gaaatattac ggatcaagta ttaccgagag aagattctga tctaaccaac
    12901 gcgaaagtgg acgagattgt acaagatgta gaagacaagg gatctaacag tgagtacgtc
    12961 aagaatgcaa ccaacaaaat tacttcagct gtagatgaaa atggtgagat cacttttact
    13021 gatcaaccag cctatgtcaa ttccaaaggg aatgtatatg tggtttatga aagtaaatcc
    13081 gctgctggat tagtcacaca aaaagcaaaa ccaatggttg tgattgcgcc aatgacagat
    13141 aaaacaggat caggattttt aaaaaatatt catttgtatc ctaagaacat tgtttctaag
    13201 ttgagttttg aattaacgaa atttggtgac gatggcgcag cccaaagcaa acaaaccctt
    13261 ttaaaagggg caaaatttga gttatacaag ggcgaacctg gaaaaggaac aaaattgggt
    13321 gatcttgtat cggatgatca aggaaagctg actgcgactg atttgacttt aggaaaatac
    13381 tattttgtgg aagttccttc ggaagttgta gtgggatcag atcaagaacc aacagcagat
    13441 caatatttat taggcgcaga cgctcgcaac gatgcgcata ataaattaac ctttgaaatt
    13501 accaatgacg gcgtgacaag tgacttaaaa gcttcctacg tgaactataa agcaccagtg
    13561 atcgataaaa cagtgacaaa tggaacagga caagaacatt ctttccaaat tggtgacgca
    13621 gtgaattatc aaggaacgat tcatatccct acagatattg caggtggaac agatgggatt
    13681 acagtaaatg gtgtgaagag tgaaacttct ccttacagcg tatttaagtg gggagataca
    13741 gcaggacaag gactttctta tgtcgctgct aaagcaaata tcaaagtaac aaataaagat
    13801 ggatctgtgg tattaaaaga aaatacagat tataaaatcc aaaattcaga aaatggcttt
    13861 gtgatcgatt ttatcgtgaa taatggccaa gtaagtgata ctgtagcaag tttacacgga
    13921 caagatcttc aaatgactta taacatgtat gtaaatgaca gcgcagctgt tgccaatcca
    13981 ttaacaaata gcgtggattt cgtttataac aacaatccat ttaatcagga agaacaccac
    14041 gaaaaaacga aagctgacgt tgtgacttat ggtgcaaaat tcttaaaagt cgatagcgga
    14101 ctatttggaa caggaatcaa agcaacaccg cttgaaggcg cagaatttgc tgctaaaaac
    14161 gcagaaggaa aatattatgg tggattagtt gatacggata aagacggtgt caaagaagca
    14221 gtatgggtag acgatgtagc aaatgcagcg atcctaaaat cagataaaga aggtcacttt
    14281 gaaatcactg gtttaactga aggtgaatat tcactggaag aaacaaaagc accaactggt
    14341 taccaaaaat taacagaaga tattgcattt aaagtaaaca aaaactcatt taaagaggaa
    14401 aatcgaatca ctgttaaaaa taatcaaaaa gcagctattc cattaacagg tagtaatggt
    14461 ttccaaacgt atgtattgat ctcttgcttg ttgctaggag caggggcact ttctgcagtt
    14521 gtttacttta agaagaaagc ctagaaggat cagctaatga ttattcgtaa gcccccaaaa
    14581 agaagagtca tgcacaaact gatgatattt ttttggttgc tgtcggcagt agcagtctat
    14641 ttaattccag ctgtggaaac aaatgtagcg aaatttcaac aacagcaaag aattttagcc
    14701 attgaaaatc ttgaaaaaac agattcttct ttggatgttg cgaaaataga gttaggcgat
    14761 cctgttggtg ttcttacaat tccaagtatt cctttaaaac taccaattta tgacggaact
    14821 tccgacaaaa ttttagagaa tggtgtaggg attacggaag gaactggcga tattacagga
    14881 ggaaacggta aaaatccttt aatcgcgggg cacagtggac tttataaaga taatcttttc
    14941 gatgatttgc cttcagtaaa aaaagggcaa aaattcttta ttaaggtaaa tgaagaacaa
    15001 catgcctatc aaatttatcg aattgaagaa gttaaagcag aagaattaca aaaaaattat
    15061 ttagattatc tcatgcctga aaaagaccaa gataggatca cgttaatgac atgcacgcca
    15121 aaaggtgtta actcacaccg ctttttagtg tatggtgaac gagtgtcatt cagtaaagaa
    15181 gatttaaaac tacagaaaaa agcaaaaaaa taggaaatca atttcattgg attggcgatt
    15241 agcactgcta ttcgcttttt ttgcgttcct attattttta tttataaaaa aataaggagg
    15301 agatcggaat gaataagaga aaatggctgt ttgtttgtgt agcagctctc tttgcaactg
    15361 cagcttactc acagatatct tttgctgata gcagtactac tccaactacc agtcagacaa
    15421 cagatagttc aacaactgca acaagtgaca cggacagttc gaagaacact gcagaagaaa
    15481 cggcagattc aagtacgagt tctactgaat cagcggaatc aacgtcagaa agttcttcag
    15541 agacttcaga aaaactgcaa gaagtagaag atacgcaagt aatcaagcaa aacattcata
    15601 ttcaaaaaat tatttcttcg aatcgaatca acaatcaagg aaggcaagtg actcagcaag
    15661 aaggtgtcaa tggtgcaaag tttattgtct acgatatcac gaatatctta gaagaaatga
    15721 cacaagatga taatgagaaa aatcaatcgg tagaaataat tgagtcaaaa ttgaaagatc
    15781 gagcaaaaaa actttcatac gatcagttga aaaaagtgac tgaaggagaa acccaaacaa
    15841 ttgatgatca ggaaggcgtg attgattttt caattgaagt acctgcaaac aaaaaacaag
    15901 catactatat cgtcaatgaa tcaagccctg aaaacatttc aaatagcgaa gatattgttt
    15961 tattgacacc tgtttcagat gaaaagggtg aatttttgac ggatgtttgg ttatatccta
    16021 aatcaaagaa atcagagccg aaagaagaaa ttaaaaaagt tgtatcaact ggtgtaaaga
    16081 aaaacttttt tgataattgt tgggattttt tcactcattt attttttaac gattaggatg
    16141 aaatgatgaa aataataaaa atgttagttt ttaatctatc ttttttagcc gcaacaatat
    16201 tgtgttgcgg tttagttttt tctcaattat caatttaccc aagatatcaa cgattgttcg
    16261 ttcattttct aaatatccag catgcttcca tgccactaga agtgattaac aagctcttaa
    16321 tcaatccaat ttttatggct agtttgtttc ttcttgcagg attggtaacg tgggtattag
    16381 gaaaaaaaga atgagaagct tacaatcgtt taacaaaata atagatggag ggcattatgg
    16441 aaaataaaat tcattgcact acgggaagag tatttacttt agtgttgttt ttgttccttc
    16501 ttctttttgc aaggggaact gcagtccagg ccttagaaaa agttgatccg agtcaaatca
    16561 aagaaaataa taatctggta ctttatcatg atagtgattc attgagaaat atcgaagtaa
    16621 ctgggatcaa tggatttaag atactagatg agtattcaac taaaaaacaa aaagtacttg
    16681 cttggccaaa agtcaaagat ttgaaaagca cgtcaaaaat tgttgcccat tatcaaaagg
    16741 tgggcactta tctaggaaaa gaaattgatt gcgacgtaac tttttctaat tttgtagcgc
    16801 acgctggggt taaccaagac ggattagcta aaacaattaa tgggaaaaag tattataatt
    16861 atttgctctt tgataaaaac atttatcgag gctttttcat gcagtcaatc aacgttatga
    16921 atgttaaact agattttaag tatgcaggat caaaaacaaa agtagctttg agtgaaccac
    16981 agccagaaat taattcaaaa atgagatcga tgagagcagc tggtattgag ttgaatgata
    17041 catttctatc tatttacagt ttaaatggac agtatcagca acgtgtgtct tggtataatg
    17101 acaataatga atctgtcggt gaacgtaata ttgatatgag agaatttgtt gggtacgaaa
    17161 aaatggatcg cttaccttat tttgtcacaa cagatacagc atgtgcagaa tacaaagctc
    17221 ctgcgttatc aacaaacaat ttaacttcaa aagtagtggg aggacgtgca gaaaaggctt
    17281 atagctcgaa tgatcatttc accgatgttg taggagctga tacttatcac agaagtggtg
    17341 taacgtatac gcttcaaggc gcttccccaa cattcatgat tggcgcaaat acgaatagta
    17401 tgatgtttag ctttgatact gcattgcttt ggacaccaca accatcgaag cctacaaaag
    17461 aagtgtttaa caaagctaat actgaagagg cagcacacaa tattgacaaa aaagtgattc
    17521 cacaaggatc agatgtttac tatcatattc accaaaagtt tgatgcatta acagtcaaca
    17581 caatgaacaa atacaaatca tttaaaatca ctgatacctt tgacagcaaa aattttgata
    17641 tggtatcgga tgggaaaaac tatgatggcg cattgtggta tgttgaagcg aatgggactt
    17701 ggaaaaaagc tgatacagga ggaaaattaa ctgtatcagg taatacagtc acttatactg
    17761 cttctgacag ctttttagct aatgctgcaa attacggtag ggactacatt ttgcagctcc
    17821 atatgagagc aaaaaatgat gcaaaaggaa ttgcaccaaa tgtggctact gtaacgttta
    17881 acaatgatcc gaacaaagga acacaaaaaa caaataaagt agaaaactat ttctctctta
    17941 aaccaaataa aaaagtggaa caagatggga aagatgttga tggtcgaaat gatggtaaaa
    18001 aaggaacgcc tacagctcct ttaaatgcag gatctgaagt acaatatcta gtcacacaaa
    18061 aatggcatac aaaaggtgta gacattgcgc tcgatcatta caagcaattt agtattcaag
    18121 atccagtgga agctcgtttg acgtataaag aaggttctgc gcgagtgata gatatgagta
    18181 caggaaaaga tattacttca gaaggtatac taacctatga tagccattca agaacgctga
    18241 aatgggaagc ttccgctgat ttcttaaata acaatctttt agatggacga gaaattcaac
    18301 tgatatttac agctaaaact cctttgaatg aagaattgga tatcgacaac caagcaaaag
    18361 tttcagtaga aagtattgag aaagcaacta atgtagtaac gattggggta aaacctaact
    18421 tacctcaggt cattgtgcca aaaactggat cgaatcattt agtgctaact gttattggaa
    18481 gtatattatt tcttacttta gcatcattgg gctatgtagt tttaaaactc aaataaatat
    18541 aaaaacaagg ctgattttca gccttgtttt cttttatttg aagattggat tcgcactaat
    18601 aaatacatta tctacgccaa ttctaccagc agagtcaact acaaaattat gacctaatcc
    18661 gagaatactt tggttattat aaagtaaatt tgcattatgg gcagggctat ttttccatcc
    18721 taccatggca tcgatagcac catcgtctga taaaaggtca tcaatataca gcccagtttg
    18781 acttatgttt tctcccttag caccatagca gcctaagctt tccaataaat cttggaaagt
    18841 accaccacca gggcgatagt gactgaagct gacttgtaat tctttagctc gaatatctgc
    18901 agcctgttga acaatatcat tgaattgcat tggggcaaca ccattttgtt ggcgataatc
    18961 atttaaccaa gcaagttctt tttgacgcat aaagttacgt aattcgtcta atgaaatctt
    19021 tttatatttg aatgtgattg ttttattttg atcgacgtta ttaatggatc gttggccgtt
    19081 tttatccacg gtaacggtag gatcgtcatt tctgcacaag aaataatttt gatcagtcat
    19141 atttttccaa ggagtagaga agctatcccc ttcgtatact gtttttgatt cactagagag
    19201 aacagcgttg gtatcagcat ttacatgatt aacagtcaca ttaaatttac gagtgtagtt
    19261 gaaaatataa ttttgacttt cagtcacatt gtttaaggtt actgaatttg ttggttctac
    19321 ccgatcgtct aaaacatatc cttgaataga aggagcagtt aaagtgacac tttcaccgtg
    19381 agtgacgttg attatctgtt ctttcaaaat atcaactcgc ccctcatttt tgtagttgtt
    19441 attgcacaac aaattgattt gatgcacttg attataattg aatgtgattg ttgtattacc
    19501 tgtgattgtt ttgctttgag tatcattacc aatcactttg tagctaaaat tatctttagc
    19561 accttcgcta tatttaaatg cttttgcctt agcagtgtaa gtagaaccag aagtcacttt
    19621 tgcttgatct gtagcaatca cttgacctgt ttgattgtca atatggttaa tggtaaccat
    19681 atattctttt ggcgtttcag gtttgtgttg caacccttta aatgcagcat caagactatt
    19741 tttagcattg tttacttgtg tttgagaaac cttagaatcg tctagtactt ttttagcatt
    19801 ggataaagca gtctgaaaat tattccaaga ttcttctgta aaatctcctt tagcagttga
    19861 ttttactcgg ttgtataaag cttgtaattc agctttattc acagtaggaa ctggtttttt
    19921 ctttaatcct ttgtaagcag aatctaattt atttttggca ctatccactt gcttttgagt
    19981 cgcttttgag tcatttaaca ctttcttagc attgtttaaa gcggtttgga atgcgttcca
    20041 ggtattgtcg gtatagtcac cttttttcgt tcccttaacg ctcgtataaa gagcttctag
    20101 agccgattta ttcactttgg gtttagaggt gtcagcgcca taccaaccga taccttcgta
    20161 tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag tgtagtgatg
    20221 tgagccagat tttacaccag gaaggaaaag gcggtagatc ggtttattat ttttttcggc
    20281 agaacgccag gcaggccctt cataagtcca gccgtaattt ttagtcaacc atttcacttc
    20341 atcccaacta gctgtgtaat ggtgatcacg aagaccttta ttgtacaaac gatagacaag
    20401 atggccaccg ctagtcggtg cttcccaggc ggggccttca taatttcccc attttatctt
    20461 tactagatgg tcacgttctt ttttgctaga ggtgtaaaaa tgttcattat tatttggatt
    20521 gtaaaggcga tacatgacgt tagttgaagc agcgtcaaca gatccagcaa gactaagtga
    20581 ggcagcaaat gttccaatag ctaaaacagt ttttggatta aataatttca ttttttcaac
    20641 tcctaaattt aattaaaaat gtttacatga ttattatata tcggttaacc gatatagtca
    20701 ataaaaaata agaaaagcct tatataattt tggcttttct tattttattt gttagttgaa
    20761 gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt tatgaatctc
    20821 atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa gggtacgcca
    20881 gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca ttcgattttc
    20941 tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata gctctttttt
    21001 gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt ttatgagtat
    21061 tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat atactaaaaa
    21121 taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc tctatgtttg
    21181 caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa gctaaagaag
    21241 ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt cttattcata
    21301 atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg catccagatc
    21361 ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga tacaaaatag
    21421 tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt attgttgttc
    21481 cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag gatgagaagc
    21541 gaacgaaaga gaaattaatt catgatggat cacaatcaaa taaatacaaa ttacaaaaga
    21601 ctagcgagtg attgctagtc tttttatttg tcctgaagga ggaatcacag tggcaaaagc
    21661 taaaacgcca tttgaaaaag ccgcgtctaa aatcttagaa cgccgcggtg tatctgaaac
    21721 acagtggaaa caagaagtcc ttgaaaaagc acaatttgac ctattacggg gaaaagacaa
    21781 ggacttagaa ggatatatct atcaacgtga acgtgaaaag cttgttttcg atgaaattat
    21841 taaacactaa aaggagcgaa caattttatg tttgaatcaa ttacaatgaa ttttttaaca
    21901 caaccgaaag tagtagctgc ggttgatatt tttagagcat tacaaactgc cgcaactagt
    21961 ttgaaaaata atctgatgat ttttgctgct gttgcggcag tggcttccat tgctcttgga
    22021 ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca cgtaggttat
    22081 gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc aatgttctca
    22141 atgtttggac aaaccccaca aggactataa aaaaacaagt agaaggagta aaaagtacgt
    22201 ggctactttt tactcctttt tttatggaag gagaatattc atgtttttta cgatttcagg
    22261 caatgcaaaa ttggataaag agttaataag aaaagcaacg aagaaagtgg gaaagcgtac
    22321 ttccttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
    22381 gttaggacaa gtgttagaac aagagaatca atcttctgtt aaattgactc tcacgttaca
    22441 tgataaagaa ggaaaaatag aaaaggctgg cacagaatac gtttttggct cactggatta
    22501 tcaaatttca gaaggttttt atcctacatt gattgattta atccgcctag atatatttaa
    22561 agatacggat gaaaccatga gtgtggaaga gtcttatcga aacgaaaaaa ttttacaagt
    22621 tttagaagaa gagttagaaa gcgaccaaag aaaagtgaat caagtgagtg aagtcgaagc
    22681 aaacgaagaa gtaccatggg aacgcccaat ctttccaaag gatgaatcaa aagaaaaaga
    22741 gaaaggaatt attgacgaac aagctgacat tttgactcat aaatcaatat ttgaggaaga
    22801 gaacgaggta atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
    22861 gacggccgaa gtagaagttg atccagttga agctaatgaa gacccaacat ggactgaagg
    22921 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagagaaaa atgaaacaga
    22981 gatacaaaag gcaacggtat ttgattatat tttggatcaa tatccgccag agcatgaatg
    23041 gatgaaagaa aaaaataaat tatttttaaa tgaactttat tcgaaatatc aattacctca
    23101 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
    23161 tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt
    23221 attagctact tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac
    23281 ggaacagtcg tcgaattggg agaaagcaaa agaggaacta aataaagaag aacagcgagt
    23341 gattgaagaa gaagtacaaa aaattaaaga gcggtacgaa aagaaacgtg acaatgcgta
    23401 ttccttgtgt gtagaaaaga tcaacaattt tactcagacg aatcaagaag cattatcgaa
    23461 agaaaaagaa aacttattaa aagaacgtat aaaagaacga aaagaagaat tttaccgagg
    23521 gttgcgtact gataagctaa ggattagcaa agaattaaat gaacaactga ataaactcta
    23581 tcaaactacg actgaagttc tgatggaaaa acacgagcag atccaacaag aattacaagc
    23641 acaaattcca aaatggaaaa aagagtatga aattcaccaa caagaattga aagaacaaga
    23701 agaaaaacaa cggaaaaaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca
    23761 ggatcttgcg gaacaaaaaa tggcgataga aaaacaacgg ctagaacaag aaaaggaaaa
    23821 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagcacagtt
    23881 aaattatctg acacatcctc aaccagtagc tgctgttcaa acgccagtga acaaccaagc
    23941 aaatttaact ccttcacccc agcgttcctt taacggatgg atggtgggat gtattgcaag
    24001 tttaagcttg cttctaggtg gaggaacagt atttgctttt aatcatatca atcaatcttc
    24061 acaggctgat tctgtagcca caacggcgtc aatcaaggaa gaagcccagg aacaagcaag
    24121 aaaagagtat gaacaacaat tagaagaatt attgaatcgt accaatcagt caacaacaga
    24181 gtcaagcatg gaaaatagca aagagagtga ttctagtaag gctaaagaag gatcaagcga
    24241 ttcaactgta caatcaaaca cttccgaaag ttcagaatca aatgaggtga aataatgcgt
    24301 gttagagaaa caacgaagcg aaaagacaaa cgattcaatt tagaaaagaa taggtatctt
    24361 tctcatttct atcgctatac tccctcagga gaagagctac cagaaaaaca tgacaaacgc
    24421 atttcgaaaa agatttttta tagtggactt gtatacctcc ctttagagta tttagtaaca
    24481 agagtaccag aagttttcaa ccgattggaa attgttaagg gaagcttaca aggaataaat
    24541 ctgtttcaat tacaggaaat gccacaggtc tttcattttg attcagctgg tttttggaat
    24601 ccatttactc ctacaactag tttgggtaca cgtttagcaa tttcgacagt ttgtgtggtt
    24661 ccagctgtac ttatgggatt gaaaatggac atgtggtttc gtcctctagc agatggacaa
    24721 aaaggggata atcgattaat gaccttaaaa gaaatccaac agatttatcc gcaaatacct
    24781 gaaacaaaaa ctacttttcc aggttatgga ggtattccaa tcacacatta caaaaaatat
    24841 tggtacattc aaacagatac tgttaatacg tgtattgttg gtacctctcg atcagggaaa
    24901 ggtcagattg aagtgttagc caccattgac aatttatctc gtgcagaaaa acaaagttca
    24961 atggtcgtta atgatccaaa aacagaatta tatgttggta gtaaagatga actagaagct
    25021 agagggtatg atgtatatgc ctttaatatc accgatccac tgcaatcaat gagtgataat
    25081 ccattggcac tcatcgtgaa atactggaaa cgaggagaca tagatactgc aacacagtta
    25141 accaatactt tcacaaatac gatttattat gatgccaacg cttcagataa caaatacttt
    25201 aacgaaaacg cgcagaaagc tgtcaatgcg cttatttttg cgttgttaga acaagctgat
    25261 caaacaggag attactccaa attgacaccg aataatctcg ttgagcttct ttctgagatt
    25321 ggcggtttta attattatcc agatccagac aatgaattca aacagttgaa tgccttagat
    25381 gaatttatga atcagttgcc acctggaaac attgcaaaaa aacaatatgg ggcaacgaaa
    25441 attggatctg ataaagcgaa gggaaatatt ttatcaacgg caatcacggg attgaatcca
    25501 tttactctaa cgaaaattgc aaagatgact tctcaatcaa cgattgatta taaatcggtt
    25561 gggtttccaa aatacttaga tttgaaatta catgaaagtc ttttaaatca acgagtcgta
    25621 gtagaatttt atcataaggg aaagaaattt catgaagaac gtgtcaaagt aggctataaa
    25681 ggatttagtg aaatcaattt tgattgtcat ttagttgcag gtgatcaagt ttgtttaaaa
    25741 tacgatcatc aaggaaaaga atatgtggct tggtatgaat tttctcaaag agtattgaaa
    25801 gacgaagagg gtaatatcgt ttatcggaaa aaacgaggag aaacccattt gccagaatta
    25861 gaaaaagaag ccgttctaac attagatgag gaaaaaagta acttattagt agagtctatt
    25921 cggatgcact ataccgataa accaatggct atttttatgc ttacacctga ttatgatcca
    25981 tctaaccata tcattgttag tatttttctt agtcagttat ataaagaatt atctacgcaa
    26041 tgtgttaaga ctaaaggaga taaatgtcac cgcaggattc attttatttt agatgaattt
    26101 gggaatagtg tgacctgttt agcctaagga taaaagaggc tagttccaac gaacattgga
    26161 ataactttac tatcgagaaa tcaaatgacg gggtaacgcc ctgaagggtt tccactgata
    26221 ctgcgacatg cgttagtagg atgtgcatcc aaacgcgtat gaagctcgct gaagtcggca
    26281 gaagggtacc gtaaggaaag acacgatacg tatctaccac gcaagtgctc tagaggtagg
    26341 gtatgtacct gatatgtcgg gtgtcctaaa aagttcaata aggtgagaat gatttgatta
    26401 tctgacaaac ttgcgaatgg aatgtctgaa acgaataact catagaaatg tgagggggaa
    26461 gggggatacg tcctctttcc attgactcga ggttgagtaa aaatgtgttt ctatgaaaaa
    26521 ccttgcatgg ttaaaggcta tggagtagtc aacaggcaaa agcaagcacc taagttgaaa
    26581 tctatggata gatagtaagg aacaggggaa gcatctagca cggagggagc ataccgaaga
    26641 tgtgtttcta aggaaaaacc gaaatcggtt ataacttagg tgaatagatg tgaaagtaga
    26701 gccacgacca gtgaaaattt cgtaatgaaa ttggaggaac aggctcaagt cgttatttat
    26761 aatataagaa acctatttaa aatcatggat tctcgtaaga ctaaaaaggt cacgaaccag
    26821 aatggagcgt gataaaacca tgactaacac aaaaggaatt agcttaaaga acaaacaact
    26881 cagacacgcc gaatactacg acatgactga gactttcgat gaattgcacg caatgagtgt
    26941 tgcaaaatca aattttaaaa atctcatgaa attcattatt gatgacaata atatctttct
    27001 agcgtataga aatatcaaaa agaataaagg aagtgcaacg gccggatacg atggtgtcac
    27061 tataaaagac attgaaaaat tggcatgtgg tgacttttta actattatta aaaggcgatt
    27121 taaggattac agaccaagaa aagttaaaag agtagagatc ccgaaaccaa acggaaaact
    27181 tcgaccttta ggaattcctt caatatggga caggatagtc cagcaatgta ttctacaaat
    27241 tttagaacca atatgcgaag caaaatttaa taagcatagc tatggattca gaccaaattg
    27301 ttctacagaa aacgccatag cagattgtct tgctagaatc aatcaaagta agatgaatta
    27361 tgttgtagat attgatataa aaggattctt tgacgaagta aaccatacca aacttatgcg
    27421 tcaaatatgg acaatgggga ttcgagataa acaactcctt gtaattattc gtaagatgtt
    27481 aaaggctccg attgttatgc gaaatggcaa tgttgttttt ccaaccaagg gtactccaca
    27541 aggagggatt ttaagtccac tgcttgcgaa tatatgcctg aatgaatttg attggtggat
    27601 agctaatcaa tgggaggaac gaactgtcaa agagctgaaa caccactatc gaaaaaacgg
    27661 agcatgggat aaaggaaata tctattcgaa attaagaagc cacacaagtt taaaagagat
    27721 gtatctggta cgctatgcag atgattttaa aattttctgc cgaacaggag aagaagccca
    27781 aagggctttt tatgccacac aaaaatggct cagagaaaga ttaaagctcc caatttcaat
    27841 ggagaaatct aaagttacga acctaaataa agctgaaagt gaattcttgg gtttttcaat
    27901 aagactccac aaaaagggaa aaaagaaagt ggctttcacg tacgtttgtc gcaaagcaga
    27961 agaaaacatt aaaaataaac taaaacaaca gattaaaaaa attcaatacg ctggagcaaa
    28021 aaaaaatagg caccgagcaa taggtgacta caacatgatg gtcatcggta tccacaatta
    28081 ctatcggata gcttcacatt gtagtgtcaa tttcactgag atttatagaa gtgttaggac
    28141 agtaatagaa gcaagactga aagctagagg aatgacgaaa aatggaatct atggacaaga
    28201 tcatgaagga taccgtccgt atctaaagtc taagcaaatg agatatattg aaggttttcc
    28261 gatacttcca attggctata ttaaaaaaag aactccactc aacaagcgga gaaatctaaa
    28321 taaatatacc aaggaaggga gaaagctcat tcatcaagag caacaagcta tagaaagctg
    28381 gaaaatcaag tatcttgtaa aaggttttag taacaataag aaatacacgg tggaattttc
    28441 tgataacaaa atatctaagt ttatttccca aaaagggaaa tgcgctatca caggaaacga
    28501 acttttgatg gaagaaattg aatgccatca cattaaccca tatcatttgt ccaaagacga
    28561 tagttacaag aaccttgtca ttatctctag agaaatccat agattaatcc atgcaactag
    28621 tgaagaaact attctgaaac tgcttcggtt actgaaacta aatcactatc aaattagaaa
    28681 actaaacacg tatcgaaaaa aagcagctaa ttttccaata tcaatcaaaa tgaattagat
    28741 aggaaacaaa ttatattgta attaacgatg gaacgccgtg tgcggtgaaa gtcgcacgca
    28801 cggtgtggac cgggggaaaa gacggagata atatcaaact cttacctatc ggaattgcca
    28861 ccaatggata atatggaagg aatcatgacg gtcacagctg gccgaaacat gttgtgggat
    28921 ctgtttattc aatcttatca gcaactttat gcgaagtatg gagaacaaaa cgcagcgatt
    28981 atcaaagaaa attgtcagac acatatttat attatgtcga cagatgataa taccatcgaa
    29041 gaattttcta aaaaagtggg gaataagacg gttgaacaag agaacagtac catcaattca
    29101 ttaggtatga atactcatat caatcgcaat gttgattcag atcgtatctt aactcctgaa
    29161 agaatttcta cttttttaga aggtgaaacg attgtactcg ctcctttaaa acgacaagat
    29221 ttgaaaggtc ataaaatacg gccgtatcct atttttaatc atggggaaac gaatcttcct
    29281 tatgcttatc aatatattga agatttcaat ccatcgtggg atatcaatga tatagacata
    29341 cattgtacac atgcggactt ggatcttaag gctttacaaa ttgattatga ttatttccta
    29401 ccagatgaat atgctagaaa tctctatcat ggactgcatg atcaattaga agaaaaaaac
    29461 gaagaaaata tgttttataa aatcgttcaa tctcttgaga atgagacggc taaaaaattg
    29521 ttaattcaat tttacgaaga acaagcagca gatcgacttc aagaatatgt tgaaaagttg
    29581 atcaaaaatg aagaaatttc tgaaatgatt gggaaacggt tgttattgga atctgaaaag
    29641 gtggtgaaag aatgatcatt ggaggaattt tttctggcat agccgactgg tggtcaggct
    29701 tattcggaga aggggatgca aaccccaaaa ccgtaacgaa agtcttacaa gattatggta
    29761 attatctgca gtatcaagat gtgttcagtt ggatttgtaa tactttttta tgggggttga
    29821 tcaaagggtt atataatctc aatactttac tagaaaaaac catttatcaa agctttgatt
    29881 taaaagactt attgaacgcc gctggggtaa atgaactatt tcaaagtttg attagtaagg
    29941 tattggctct tttttgtgtc gtcacattaa tttatttagg cttgaaattt tttatgagta
    30001 aacacccacc aaaagtcaaa aatatcttgg ttaatttatt tatggccatg atcttgattt
    30061 ttggtggtag ttctttaatc gatgaagggt taaatatcag taaaagcttt tacggagacg
    30121 tcactgcagc aaataaagca gataaaacgg gttcacctgc ttttcaaatt attcaaaata
    30181 atgtgatcga tgtcagtaca ttactagcaa ctgacccgaa taaagtagaa tccctgccaa
    30241 cagacaaaag aaatgcttta acagcgaaaa attttaaaac agcgaatatc aatgggatta
    30301 ttactcctaa acaagctaaa gaaatggcgg ataaagataa gagtggttta tcagatttac
    30361 aaaaggatcg gttaagagcg ttacaatacc gactggaact ggatgataat ggaaaagaaa
    30421 ttcctgtgca aatcagtgat gaaggcttag ccaaatatgt ttatacttca gggtatcgtc
    30481 gatacattag tagtccaggg attattttag ctggtgaatc ttctttggcg gtggcctatt
    30541 tatttatcct atttacgatc gttacttgca ttattgagct ggtatttaaa aaattctatt
    30601 tagtgattgc cgcgtcaacg gactttgaga caggacaacg gatgaaaagt gctatcgaag
    30661 atatttctca atccttcttg ttattggcat ttacaatttt agaattacga atctatacct
    30721 taatgctttc aggtattggt gatttgcatg cggcaggaaa gatcaatgga ttcttgtatg
    30781 ttgtcgcttt gattgtacta accattgcgc tattcaaagg ctctcaatca gttacgaaaa
    30841 ttttcggcgt tgatacgagt ctgaaaaacg gcagtaattc tcttcttagc atgtttgcct
    30901 taggaaatat tgcgaaaaat gtgggtagcg gagctaaaaa ccttgcaagt gctggtaaga
    30961 gtggtttaag ccatttaaat gatattcgta aaaatggatt tggccatcga aaagatgtta
    31021 cccctgataa cggggaagaa caatcagacg ataaagctac aactgtacct aatagtaaga
    31081 aaacagtttc cgaaatgttc gggaaagcga aaaatggtgt ttctaaagcc gcagaaggtc
    31141 ttggttacgt caatgaacgt gggttaaaag gaacagctga agatcttaca gaaaaagcca
    31201 aagacacctt agaagataaa ttagacggca cgagagctaa agaattggct gaagctgtga
    31261 agaatccatc cgaagcacta gaaaaacaaa aagaaaaagc ttcagaagtc aaagaaggac
    31321 taaaagatgc gtatggtgaa ggacaagtca atgcagcagt gaaagctaat acaacaaccg
    31381 aaaaagaaaa tcaagaaggt atagtgattc cttcagtcaa agatcaccaa gaagcgttgc
    31441 actctaatga aaattcgatt gaagagaaaa attccataga agaaagaaat tcagaagaag
    31501 gaaaaaatgg acagttacca aaaactgatc cagaacaata tcgctataaa cctaacagtg
    31561 accttgaaaa tgagaagcga atttcagatt tatcaaaagt aaatcaagaa gacaaagcag
    31621 aaaatgaggg acagttacca aaaactgatc cagaacaata tcggtataga tctgttaact
    31681 ccgaaaatga aaaacgcatt tcagatttac caaaacgaaa tcaagaagta caaacggatg
    31741 aggattccat cgttctgcca aatgataatg gaatcgaagt tggaactgaa aaatcaacga
    31801 aaattggttt acctaaggca caaaatttac caatgaaaga tagtcgtttg aatcaattca
    31861 aggataatga tcaaagtttt gttcatcaat cacctgcagg tactgttcaa atgaaaccaa
    31921 gtcgtttgcc tgaaaatgga aatctgacaa ctgtttctta caatttatct gaaagtggtg
    31981 agacagggaa tgcgtccgtt caaaatgttg tgacaagaga agctgcacca aaacctattc
    32041 aaaacgttca tacaggttac aagccagcaa atgtggcatt tactgcacca acaaaaataa
    32101 atgtgacaag agaaactaaa aacaggaaca atgcattgaa agaacaacta ttgagggata
    32161 tcaaacagga tatcaaacca taaaaggcct acaagttagg ccttttcttt atatgaaaag
    32221 gagaaatcaa ttatggataa atatataaac atgaaaaacc ctccaagatt tagtggagaa
    32281 tggaagtttt tcgctttttt ctttttagat ctattaacgc ttggtggctg ctttttttgg
    32341 gcgaaaatgg tcaataatct tgtccactta ccactctttt ttgcactctt gaattatggc
    32401 ttatggattg cgatagcgtt agtgattgtt tggcgaccct ataataaccc aggtcaacga
    32461 caaatttttg cgattatcga gtctattttt tttgcggaca agaaccatta tcatgcgtta
    32521 gatcctaacc gaaattatca tggtacgtat aaggaggatg aataaatgcg agtcaaagga
    32581 caaaattcag ttgaaaggaa atccattttg cagtttggaa aaccagaaga gcgctctatc
    32641 ttggacttat ttccgtataa atcctgtaca gaagagggag tcttaattac tagagaagat
    32701 cattttcaac gttattatcg tgtagtttct acagacgtgg aaggattaaa tgaacaagag
    32761 aaactagaac gaatgaatca gttgaccacc gtcatgcgta cgtatgtgcc aacaattaag
    32821 ttagttagtt tgaccacaga gacggatcta acggaacaaa tcgtgcaaaa gcggcagtta
    32881 ttagataaaa atcgtatgga acagtcctta ggaagaaatc tacagcgatt aagaaaatat
    32941 gaaaagaaat tagtagagga aatccaggaa ttgaaacgag cagaacagga acgccctgat
    33001 ttaagcttct ttttcgtcat tgaaggaaag acaatcaagg atttgcaaac cagaaaccgt
    33061 cagttaatgc gatctagtgg cattttaggg ttaaaaccac tctacaaaaa agaattgatc
    33121 aagattcttt atcgaatgaa taatatgaat gatgagtagg aatggggaag agagaatgaa
    33181 atatccgttg atatggttag aaaaagatgt tccgaaagaa gagaccacta gaaaatttcc
    33241 tcacacttta agcctgggta agttgggaaa aggaaaatct tttaaccaag taaagaatca
    33301 aacaagaaga ggtgactgta catgttagaa gcaattaaaa cagctttttc accacaaaat
    33361 gatcaaaagg tacttgatcc attaaagcaa ctaggaaatc gggaaataga aattttaaaa
    33421 tccaaagggt atgatttaga ctttttaaaa caagtgatgc cacaaggtgg attaaatttc
    33481 gatgaagagt atgtgaccta tggaaatggt tattcacgat gtgtgacagt ttatcactac
    33541 acaaaaaatc ctacgctttt ttggttggcc aatctcatga acaatcctca tacagtggca
    33601 acactagatg ttagcacaga tgaaaagcaa aaagtgattg atcaaatcaa tcgtgcctta
    33661 gatgaattaa atgatcggtc agataatgaa cgaaaagcga ctgaccgaaa taaaagcgcg
    33721 gaagaatacc tagaactagc ggaatatgcc cgaaaattga cttctaatgg ggaaatttct
    33781 aaacaagtca aagtgagagt acatgtttac ggaaacacga ttcaagaagt agatgaacga
    33841 gcacaggaac tgatcgaaaa tctcaaaggg atggattatc gtgcagtgac ctatctgcac
    33901 aaaacgaaaa gagactggca atcgatgttc accatgttcg atgaacaagc tgaatggttt
    33961 ggaaatatca aagggcaatc actaccatcg ctaaacattg gcggaggttt cccgttccac
    34021 caccaaacgc taaaagatcc aggtggaaca caatatgggg aaaccatgac aggcggcccg
    34081 tttgtttgga atgccacgta taaaaatagt gtgcggttgt ctaccaatac cttgcttcta
    34141 ggaatgatgg gagctggtaa atcgaccgtt cttaaaatga tcgcggaagc ccatttagct
    34201 gcaggagact ttatttgggg ctttgaaaag ggaaaagact ttattccctt tttaaaggaa
    34261 tataacggca ttatggtgcg tttagacgga tctgacggga tgatcaatcc tttagaaata
    34321 tttgccacac gtacgtatga tgaagcttcc agtttatatg acgatggatc agtcaaagat
    34381 ttgaagatca atgaagcagc gtcctatcaa acgcacttag acaaagtggt ctatcaggta
    34441 cagttagtga gtcctcagct aaaaggaacg atgaaagcag aatttaagac ctatttaaat
    34501 cgtttttatg aagagtatgg aacagtacca cgcggcttta cttcctcaaa ttcgcgttca
    34561 aatactgata cgcaagtcac aggtaaagat ccagaagctt atccgacgtt caaagaattt
    34621 ttagactttt taggacaact ggaattacca ggtgcttccc aagagaaaaa gaatcgaaaa
    34681 gaagaaatgg aatccatcgt agaatcctta tgtgagacgt atggcatgat ttttgatggt
    34741 catagtacga ttcgacacct tgaccaacaa cagttggtct tttttgacat tgactctatt
    34801 acaggcttag atcgaggggt acaacaatgt caaatgtaca tggctatgac gttgatttgg
    34861 aatcaggcat tgattcaagg aagaagacaa cgacgtttaa ttaaagaaaa acaaatggaa
    34921 gaacaagact tcaaatgctt cttggcgatg attgatgagt gccataatgg tgcgacctgt
    34981 tctgcagtga aaagctgtgg gttgcaagta gtctaaaaac tattgtaata actcttataa
    35041 cgagaagtca aatgagtggg taacgccaca aagggcttcc actgatactc cgacatgcaa
    35101 caagtagttt atgcagacca acgttgtgtg aagctcggtg aagtcggctg aaggataccg
    35161 taaggtaaaa gaaatagtga ataccacgaa agttctctag aggtaaaggg tgtatccgat
    35221 aagtcgggtg tccaaaaaaa cttaatatgg tgagtatgta caaacgtact gacgaactag
    35281 cgaatgtact gtccgaaagg catagctcct agaaatggga gagacagtga gcaaaaaatc
    35341 actcattgtg ctaacttgtg gttgagtaaa agtcgttgtt atgaaagacc atgctgcgtt
    35401 acaggcgtag gagaagttag ggggcaaaag ctagcatcta agttaaggca tgtggataga
    35461 tataagggaa caggggaagc catcaacatg gagagtgcat actcgatgaa gtggtagtaa
    35521 gaaatatgtg aatgacaaat aacttacttt agtgatggtg aaagtagagt cacgactgat
    35581 gaaggttctg taatggaact ggaggaacag gctcgattcg tctgtaaaag tcatgaaacc
    35641 cgttcaacca tgggattctc gtatgactaa aaaggtcacg agccaattgg agcgtgataa
    35701 accatgacaa aagaaacaag tccaaaagat aggaagttgc ggcatgcaga atattatggc
    35761 atgacggaaa tcttcgataa gatatactcc aaaagtttga agggagataa tttcaagaat
    35821 ctaatgcaga ttatcatttc tgataacaac attgtcctag catatcgtaa tatcaagaga
    35881 aatcacggta gtgtgacaag aggagaagat ggagtaacga ttaaagatat tgaaaatctc
    35941 aaccgtaatg aattcgtaga aattgttagg aagcgattcg cacattataa ccctcggaaa
    36001 gtaaggcgtg tagaaattcc taaatataat ggtaaattaa gaccgttggg tattccatca
    36061 atatgggata gaatcgcgca gcaatgcatc ttacaagtat tagaaccaat atgtgaagca
    36121 aaattcaata atcacagcta tggtttccgc ccaaatagat cagcagaaca cgcaatagca
    36181 gattgtatgg cccgaatgaa tcaaagtcac atggaatatg tggtagatgt agatatcaaa
    36241 ggtttcttcg acgaagtcaa tcatactaaa ctcatgagac aaatctggtc tatgggtata
    36301 cgagacaaac agcttctctt aatcattagg aaaatgttaa gagcacccgt agtacttcca
    36361 aatgggaagt tgatataccc aacaaaagga acaccacaag gcggaatact tagcccatta
    36421 cttgcgaaca tcaatctcaa cgaatttgac tggtggatag caaatcagtg ggaggaaaaa
    36481 gatgccaaag agctaaaaca tcattttagg aaaaatggag cttgggataa aggtaatatc
    36541 tatcacaagc tcagaaagag tacaactcta aaagaaatat acctagtgag atacgcagat
    36601 gattttaaaa ttttctgtag aaattataat gaagcgaaaa aagttttcta tgcaactaaa
    36661 atgtggttac aggagcgtct aaagttacca atatctgaag aaaagtcaaa aatcacaaat
    36721 ctttcaaaag aagaaagtga atttttaggc tttacgatga aattagtgac caaaggtaac
    36781 aaaaaggttg ttaatacgca tataggtaca aaagcacaaa gcatgattaa agtgaaacta
    36841 aaaaaacaaa tcaaacgaat tcaaaaaagt cctaatagca aagatacctt tatctatatt
    36901 ggagtctata acagtatggt tattgggatt cataattact tcaaaattgc gactcattgt
    36961 agtgtagcaa tgcgaaaaat tgcaagagaa ctcagaccaa gttttgaaat acgactacga
    37021 gaacaaggaa tctcaaaaga aggacaatac ggaaagtacg aaggatacaa gccttaccta
    37081 cggtcaggac aaatacgata tataagaggg tttccaatat taccagtagg gtatattcaa
    37141 cacagacatc cgatgtgtaa aaaaagaaat attaataaat acaccgaaga aggtagggaa
    37201 gccattcata agaagcaaca aaccgtagaa gaatggaaga tatcgtggtt gtgtagcaat
    37261 ccagtgctta gtaagagagc aacgattgaa tttgcggaca atcgaatttc aaaattcatc
    37321 gctcaaaagg gaagatgtgc agtaacaggt gaggagctta tcttatctga aatgcattgt
    37381 catcatatta tcccttatca tgaaagcaaa agtgatagct atgaaaacct agtgattgtt
    37441 actgaggagg ttcatcgggt aatacacgct acacaaagtg aaacgattga ggaacttcta
    37501 aaatatctaa aattaaatcc aaaacaaaaa gaaaaactca atgagttgag attaaaagtt
    37561 ggtaacgaag aaatatctta ataaatattt gtaggacggg aaaacagaat gtcttttata
    37621 gacgagggaa cgccgtatgc ggtgaaagtc gcacgtacgg tgtggactgg gggaaaagac
    37681 ggagataata tcaaactctt acctatcagt atcgattaat ccagatttta ttgaaacggt
    37741 aaattatgtc acaaactttc aacgagaaat gcgtaaagta gaagcaatga ctgttttagc
    37801 tacacaatcc ccacaagaaa tggtgccaga aaatatgtct tctgataact tctcgaaact
    37861 gaagaaagtc tttgaatttt cttctacgaa gatctttatg cgtatggatg aatctatttt
    37921 acgtcatata gaaggattag ttggtaaatc aatgacaaac tcagaattag aatccattcc
    37981 tcaacaaaat caaggagacg ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca
    38041 ttttagtccg aacaaacgtc aattaaaatt gtttgatgga ggaaaataat ggaaaaccaa
    38101 aatgaaagtt taatcaagca gtatgttaag cgtcgtgcaa agcgacgctt atttttgtgg
    38161 ttgtttggta cttctgcagg tttgattact attttgatta ctgtgttcgt gacccttttt
    38221 ctaatacttg cggcgggttc gattgataat tctgattcag attcaagtag tggtggcgaa
    38281 gcttttacag gagaatattc ggaaggctta ccgatttata aagaaatcaa aggaagaggc
    38341 cctttttctg atgagattgc gcaatatgct gtaggtgcag cagtgaaata taaattatta
    38401 ccaagtgtga tcctttcgca atacggttat gaatcagcgt ttggtacgtc ggcgtccgca
    38461 agaaatgatc tcaactattt tgggattact tggtttgatg gttgtctgtt tcctaaagga
    38521 actgcgcgag gaatcggtgg tattgaaggt ggctggtaca tgaagtttcc caactcaaaa
    38581 gcagccttct cctattacgg ctttatggta gccacacaat ccaattttaa tgcatgtgta
    38641 ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggtggtta tgccgctgca
    38701 ggaattactg aagatagtcc gtattataca ggttgtatgt ccattatcac ttctaataaa
    38761 ttaaccgaat atgatgaatt cgccatcaaa cattggggtg aaggtggcaa taacaacggc
    38821 acgatcactg gtgaatggac gaatccattc cctggcagca gtttagataa aagttccttc
    38881 agtggtggtc aattatttgg aacaaatcca ggcggtgaat tccgtccgaa tggcttccat
    38941 gatggattag actttggttc agtcgatcat ccaggaagtg aaattcacgc ggtgcatgga
    39001 ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt taggtgcatg tgtgatcgtg
    39061 attaattacg acggtttgaa tatggtatat caagaatttg cgaatagtac aggaaattct
    39121 cgtgtgaagg ttggcgatca agtgaaagtt ggtcaagtaa tcgggatacg agatacagca
    39181 cacttgcatt taggtttcac tcgaatggat tggcgtcaag cacaaggaca cgcattcaca
    39241 gacgatggta catggattga tccattacca tctttgaata gcagcaaaaa atagatcagg
    39301 agaaaaaata tggaaaataa aactttaaaa atcgtcttaa cagctgcaat tagcgttagt
    39361 ttaatcggca atatcgcgtt gttttggcag ctgaaagaag aacaagagac aaataccttt
    39421 aaaattgaag aagtggctaa aaacgcgtct gagaagctca aaaaaaattc ctctgaggca
    39481 caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga aatcgatcag
    39541 ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca aagaaagacg
    39601 gcagaagagt tcggtcaatt ggccattgat aaatcattaa gtagaaatga attaacggaa
    39661 aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctgc agacgatgat
    39721 caccaacatg atgattacgg aagttactcc tttacattag gtgacgtgca aacttatgct
    39781 caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta tacaatcggc
    39841 aatccgcaat tcaaagatgt caaaccacaa aaaataaaag gtggattaac tgtcactgaa
    39901 aaacaagtag atggtcaatg gaaagtcata gactttagtt atttcactcg ttaggaggaa
    39961 gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa aaacgaaaaa gttatcttca
    40021 atggctggga atctttatga ttctcggctt tttgattctt gctagtccga tttatacgaa
    40081 tcagaaggta gatataccag atgaggtaag cacgaacgcc tttttaaaca cgaaagagct
    40141 gacaatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat ttgcgatcga
    40201 tgatgaagat caaagcttgt taaaagaact ggctaatctt aattatcgcg tagaagtaaa
    40261 gacagctaaa ggggattatc aagcaatacg agccaaagtg atcaaggtgt ctgatgatta
    40321 ttttgcagtg aagctaacgc atgtaccaga agaatatatc gccatgcgtt taaccattat
    40381 tcctgaaaaa attgatccaa aagtggatat gcaagaacca caagatctga tttattacgt
    40441 gcacgaagat aaggttaaag atcaagtgaa agatgaagat tatgaacaac acgcgatcaa
    40501 ttacaaagtg aaaggataca agaaagaaca aaaagcagct caaaaacaaa tggaatcttt
    40561 ccaagcaacc attgatttga atgaagaatt agtgaagaag ctaaaagatc aattgcctta
    40621 tcaagtcgca gatgatcagg aaaatactga aaacaagatt aatagctacc agcaagaaat
    40681 tgaaacatcg aaaacgcaac tgaaagatca agaagagatc attcaaaaac ttcaagaaaa
    40741 aattgatcgt atgacaaaag aaaagattta gaaggacagg tggagtgaaa aaatgaagcg
    40801 aacaaacttg gctgacaaag atactttcgc tgagcagaaa gtattgatta aagcctatct
    40861 caaagttgta aaagctaaag aaatatgtcg agaagtgaat ttatctaaca caacaatatc
    40921 tgagttgaag aatggtcgaa agaatttaga tactttaaaa ttcagatcat ttgaagctct
    40981 atatgtagct gctaaaaaat attttacagc tgataacaaa taaaaaacag gagtatttta
    41041 tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat atcaccttaa ccatttgtta
    41101 atgagatggg agaaaacatt aaccacgatt ctaacgcaaa catcaagcac ataaaacatt
    41161 taacactcct cctttctggc tgaagatttc aaccaaacag gaagacagcg tatcaattat
    41221 agcatacact ttttcttaaa ttcatttttc atatgacaaa atatttcctt ctaatcgaca
    41281 aagttagaag gattttttat tgtctaaata cacaaataac cttaattttt aaagagcata
    41341 aggaaacgtg atatcacttt ctcacgaaag gtgttaggta ggctgtgcct accacgtttc
    41401 taagaaagat aaatgatatc aaaattttgg atttgtgtgt agatttgtgg gtaggatgaa
    41461 agcgaatgtg ggcagtaaaa taagcgtttg tgtgtagcaa aatcaaaaaa tgtgtaggga
    41521 tatgtgtagc tacacatatc cctacacata ataaatttgg aggaaaatat ggacttatta
    41581 attagaaata ttgaagaaaa gtatattaat aaaattgaca aacgttgtga agaacttacg
    41641 gtcaagactg gcaaaaaatg gagtcgaaat caatacctaa aagttttgat cgaaaatgat
    41701 tttgatcacg ctcttctgaa ttataaaaaa gaccaatttg atcggttgtt agaaaaattt
    41761 gtggatattc aaacttataa tacgaaaact ttagaagaat atattgcaac aaataatcaa
    41821 ttaattggtt tgttaattga ataataaaaa tagtattgtg aatttataaa aggaggaaat
    41881 caattttggc aaaagcaaca agaaaacatg tatatcttta cgatatgcat atcgaagtac
    41941 ttgatgaaat aatttctact catgaagata tatctaatta tagtgaagca gtcagacatt
    42001 tgtgtatgat gtatgatcca aaaatcgaag aaaaaataaa tccaattcaa attaaattga
    42061 atgcaatggg aaaagaaatt tctattttaa ccgaaatggt ttcagagttt gcggatcttc
    42121 atttgaaaga tacaggaata ctaattggta caaattcaac tgtttatcaa gaagcagaag
    42181 aacgtgtgga aggaaaaatt aaatccaatc aaaccaaaaa atattcttct aagttaaaga
    42241 aaccagtgaa tgaatctgtt cataccagag tagatccatt tgatccaacg aagattctaa
    42301 aaagataaaa gtagaaggag aacgtgatga ataaatcgcc tgcagttatt cttacttgtc
    42361 aatttacctt gcccaataaa aaatcttttt caacatacat tgattatatg acaagagaaa
    42421 aagcactgga agaaatggaa cgacgtacgc ccgaagaaga acaggaactg caacgaatca
    42481 agaatgcttt agaggatttt tatattcctg aaggagaaac tttttcggaa agtaagacgg
    42541 ataaagaatt cagtgaaacg tcgaatgaag ctcaatcgtt gatggaacat ggaatcaatt
    42601 ttgacaagtt agaggaacaa gattttacca aatatctttc ttatatgacc cgtcactatg
    42661 cgttagaaca aaagaaagaa ctgacgcaaa gggaacaaaa agaataccaa cggttgaacc
    42721 aagcaatgaa aaaatatcaa acagagccac ataaaaaagc ttcaggaaat cttcctggag
    42781 ttttttctat ggcttcagac gaagtgaaag gaagtgatct gaaggagatc aaacaaatct
    42841 tccaaagagg acaacaaaaa ggctcaatca tttatcaaga tgttatctct cacgacaatt
    42901 cgtatcttga aaaaatgggc ttgtacgatc ccaaaacaga taccttagat gaagaaggtt
    42961 tgaaagcagc tggaagaaaa atgatggaga cattattcga gaaagaacaa ttgaatgaaa
    43021 caggttactg gatggcaacg attcaccgta acactaagca tatccatatt cattttgcag
    43081 ttgttgaaaa agaaaatacg cgtaaaccgt atactgagat tgaaaatggt gtgagttata
    43141 tcgtaccgaa ggggaagaga agacaagcaa cattagacgc aatgaaaacc aattatgtac
    43201 atgagttaga acgtttctct tatgaaaagg atcgaatgat cggtatggag aaacgaaatt
    43261 tattgacacg aaaaagtgat ttgcgtaaca ccttaacaaa agtagtaaaa gaaccagcac
    43321 gttacgatcg acacgccatg aacttattaa atgaagtata taaaacccta cctgaaaaac
    43381 gttcggaatg gaattatgga gatgaaaatc gaaccaagct ttcaccattt acaaggggaa
    43441 aacttgatga gttaaccaaa tatgttttaa caaaagaacc tggatatcaa gaatatatac
    43501 agatttcaaa agcattgaaa gaagaagcca aaagccttta tggggaaagc aaaagagaaa
    43561 caaagaatgc agaaaaaaac gccctctttg atttaaaaaa gcgcacagga aatgcgattt
    43621 taacagaact gaagagacag gatagtcaaa taaaaccatt aatagaaagt gtcaatcaga
    43681 tgcaatctat ttacaatgaa cgatcctttc gaacactcaa acagttgcca acaaatgaat
    43741 acaagtatag ttatcaagaa ttcgtaaagc gtaggaaaca attccaacaa agagttgtca
    43801 cgcagcgaag cttgcgtcaa ttaaatcgaa caattaatgc acatgaagaa aaataccacg
    43861 caagtaaagc atatgaagag caacagcgaa gaatccaaat ggaacaagaa agacaatagg
    43921 aggaagagaa atgaaacaat atttagatca atggaaagtc atagaaggat cactaaaaga
    43981 agaacgaatc gaacaacttc ctgattgttt agaaaaagag catttgtttc agataagcga
    44041 aatgttgaga aacgaacagt ttgatccaaa tcaattttta gttgttgagt acccagctac
    44101 tggtgtgtat tgttgtaatc atgtgaaggg tgaaaaatat tttattattc aagaatacga
    44161 aggtaagtta gcgccttatt atactacttg ggaaataaat gaagagggaa tcaataattt
    44221 cccatgtgaa agcattgaag agtctatttc tctcacggaa tgttaaataa atagagacgt
    44281 aaaagagtcc tgaacgtgat tgtccaggac tctttttcaa tgaattgttt tccgccaatt
    44341 cactgagtgg tgcgattcaa ttttattata tcaaaagaaa gtgagattcc aaagtgaagg
    44401 gaggaagaag aaatgactca gacaacgatt ggtgttgtgg ttgaaaaaag cagggataat
    44461 ctcgtttttg tgacagaaat tcaaacaggt agagcttttg tagtcacaga caaagctgca
    44521 aaagcgtatc aaaatggcaa tattttaacg ttaaatctga ctacgaagat gttcgttgat
    44581 gcagtagaaa attatccatt tgtttagaca aacgatattt tacaaggagg aaacggacat
    44641 ggcagaagaa aaagaaccac tagataatag tcgtttggga aagtccaaaa gaaaattagt
    44701 acggttgcaa aacgaattaa ataatcaatt tcaaaagcta tttgatcacc aaaaattgac
    44761 aaatgggcaa ccaatgaatg acaaacgaaa tggtcgttca tggtttaacc agcaagaaaa
    44821 aatagaaaat aaaatacaaa gtttgagaga agagatcaag caacaagaaa aacaggtaga
    44881 aaaacttgaa agacaagaag agctaaagga aatgggttat aacaagtacg gtggcctgga
    44941 catgacgatc gaaaatattc caagaatcaa agaagaaatc gaacgctttg aaaaaggtga
    45001 atcgactttt tcagctgcca cgatccgaaa ataccaacga aaattggaaa cattagaaca
    45061 gctgaaagaa aaatcagaga aaggaaaaga aaatcttctt cctgaagtac aagcgatcat
    45121 tgacagcgga cgagtgacgc aatggaaaaa gaatccaact atctattttc tgaaaggcta
    45181 tcgaaaagtc gctttagaac tcaatgaaaa cgggggattt gaggaatcta aaaaatacca
    45241 agccacgtcg gaagaagaaa aggcagctgt tcgtgaatta cttgaagaag ctacacctac
    45301 tgattctgtg aaggagaaag aaaccgatca tttctatcga gtagaattca acgaaaatga
    45361 tccagcaaaa gggttgaaaa gttatggtgg ggagatcgta acactagaat tgatcgatca
    45421 actcaaaaca ctggataacc aatacgccgc agaagcaggc tattataaat tctattttga
    45481 agaagtgaag aatggagaag tagtcgatca gatacgaatg gatctagggg atggcgtcaa
    45541 tagtaatcac cccatctatt tagagttagc tagattgtgc gaacaatcac ctgaaaagga
    45601 acaagaacaa acagtcaaaa atgaacctat ttcaaaagta caaaacgaga ttaaagagac
    45661 aaaaacaaaa caaaagggtt atcaaaaacg aacggcagaa gagattaagc aagaagtcaa
    45721 atcactttcc caacaggcgc ttgatgcggt gaaaaagcat actcattcac ctgaagacgt
    45781 caaagaactt ttgaatttta tgtctcgttt tccagaacga tcctttagaa atcaattatt
    45841 gatcgaggaa caatttccag gcgcaacggc ttgtttaggt cgtgccgcgt tgaaaaaaga
    45901 aggaatctat atcaaaaaag gagaacaagg gaacaagatc tttgtcagaa aaacggtcaa
    45961 aggattttat gagaaaggac gtggctttgt tcgagaaaca gaggctacac cagaagataa
    46021 aaaacgaatc gaaagtggtc aaatcgaagt gatcaaaaaa ccttactaca cgattgaaaa
    46081 agtgtttgat atcacacaaa cacaattgaa acctgaagac tatccgaaaa tcttcccgaa
    46141 ccgcgtcttt gattttcaat tggatcaaac agggcagaca gaacttcaag ttgggattca
    46201 agcggtagcc aaaaatattg gaattcaaat tcgagatatg acagaaagtg aagtctatca
    46261 aagagaatta ggacaagcac gtggggcgta cgttcgtaac gaatttactg gcgaagaaga
    46321 aatcgtcatg aatactcgaa atactcctac gcagcattta gccacaagta ttcacgaatt
    46381 agcccatgct agaatgcaca aattttcaga attagatact gcaacaaaag aactacaggc
    46441 agaaatgacc tcctatattg tgtgcaagca ttttgggatg gacacctcgg agaaagctat
    46501 tccttatatt gcgacatgga cgaagaatag tcagacgttg gatgataagg aagctgcgga
    46561 acgtgggaaa atcatgaatg atgtcagtcg tgtcgcaaat gaattcattc aaaccatttc
    46621 taatgaaatc aatcaataca gagaaagaga accagagatc cagccagcag aagtgaagca
    46681 ggagcataaa aatactgtag aaacggcaga acaaaagaat attgtgcccg ttggctcact
    46741 ttggatcgac aagaaagacg ggaaagtcct ggaagaagac acgggccgaa cgttacattt
    46801 gaaggatgcg gcgtttattg aagatccgca gcataatcaa gtgattttaa aaggaaaaag
    46861 ttatggaaaa gagatccaaa cggcgttacc ttcagaagaa tttgcgctag gaaatccatt
    46921 ccgattttca cttgagacag tgtcagaagt ggcaaaacga aaaaatgaaa tctattatga
    46981 acaacaattg tcacaaaaag aacgcgtata aaacaagcaa gaaagactat ctgaaaaatg
    47041 gatagtcttt tttcttttgg gaggtttgaa aatgacagag aaaaaaatga gtctcattga
    47101 tcgatgcaag caaatggata ttgtggattt tgcacgaaac aatggtttag cggtggccaa
    47161 taaaggacga gactatcgct tagaagacca tgattcattt gtttttgatc gaagaaaaca
    47221 acgtttttat tggaacagtc aaaatattag tggcgacatt attgaattag ctaaactttt
    47281 tttcatagac aaagagatcc aagaccctaa gcaacaattt aaagcagcca ctgattttat
    47341 tctaaagaat gaagacaaaa cagaacgagt ggaaaatctt cattttgaga cagaaaagta
    47401 caaagatcac cccgttgatt atcaaccact cacagaaaaa ggaaggaatt atctgaagga
    47461 agaacgaaaa ctgcccgatt ggttgattga ttatgcagaa aaagaaggat tgattgccga
    47521 actaaagcca aaacatgaac ggcaaaattt cttagttcga gacgatcgtt tggatcatgc
    47581 ggtcgccttt ttatggaaag atccacagac aaaagagaca gtaggggcga gttatcaagg
    47641 aacgttcatt gattatgaac gttttggtga gagaggcaca tataagcata tagataaaaa
    47701 ttcgacagcg aatcatggct ttaacctaaa gattggtgat cctaaacagt taaaattttt
    47761 tgagagtagt attgatttac ttagctacgc tgcactgaat cgtgatcagt taaacgatac
    47821 gtggttagtc tctatggaag ggttaaaaca ccatgtgatc agtcattatt ttggtgaggc
    47881 agtatctgaa ttgagaaaga aacaagcctt tcctcaatcc atcgagattt gtgtggataa
    47941 tgaccgtgca ggacatattt tctacgaaaa agaacaatta atgggcgcag ttgatccatt
    48001 tacgaatcag aaagtacgtt gtgaacgagg aatcgctaat gattggcaag tgccaagaga
    48061 atacaaagtc atttatgaag aagtcgcaaa agaagaaaag gtcacaccag aagctattat
    48121 ggccatacac aagacagaaa acaatctgca attaacgaac caactggttt ccgctcacaa
    48181 agtcaatgcg tcttttggtc agcagctttc tgttaacgat tcaatagaag tcattagttt
    48241 gaaagatatt tgtagagaag tcgcaaaaga attaaaagat tgtgaacgct cgaatggtac
    48301 gtatgatttt gatcgttttt atcaaggaaa aggggatatt aattctcaga ttcttttctc
    48361 ttataaagca gaacaatact acaaaggcta taaaaatcat gaacatgaat ttgtacctga
    48421 agtgaaaaag gattggaacg atcaattgaa gcatgagatc catcaacagg aaattagaaa
    48481 gcaaaaacgt gcgatgttgt ttcaacaagg cagacaacaa gaaagggagt gagatcgtca
    48541 cgatgaaaac tgtcatttta gcagaaaaac cgtcacaagc gaaggcctat gccgatagct
    48601 tttctaaagc cacccgaaaa gacgggtatt ttgaaatcca agatcgattg ttttcgggtg
    48661 aaactgtgat cacgtatggt tttggtcatt tagtcgagtt agattcgccc gatatgtacg
    48721 atgaaaattg gaaacaatgg tcattagagc atttgcctat tttccctaca cattatcact
    48781 atcatgttcc taaagataag aaaaagcaat tcaacgtggt gaaacagcaa cttcaatcgg
    48841 ccgacaccat tattattgcg acagatagcg atcgtgaagg ggaactgatc gcttggacga
    48901 tcattcaaca agcaggcgct gatcaaggaa aaacctttaa aagactttgg atcaactcct
    48961 tagaaaaaga agccatttat cagggattcc agcaattacg agacgcagaa gagacctatc
    49021 ccaaatttga agaagcacaa gcacgccaga tcgctgattg gttgatcggc atgaacggca
    49081 gcccactgta tagtttactc ttgcagcaaa agggcatacc aggaagtttt tccttaggca
    49141 gagtccaaac cccaacgctt tacatgattt atcagctgca ggaaaagatt agaaacttcc
    49201 aaaaagagcc ttattttgaa gggaaagccc aagtaaccgc tcaaaatggc atatttgatg
    49261 cgaagcttga tcccaatgaa acccaagcga cgcaagaagc atttgaagcc tatttaaaag
    49321 aaaaaggtgt tcaagtagga aagcagccag gcacgattct tgaagtcgaa accgagaaaa
    49381 aaagcgcggc aagtccacgt cttttttctc tatccagcct tcagtctaaa atgaatcagc
    49441 tcatgaaagc tagtgccaaa gatactttgg aagccatgca agggttgtat gaagggaaat
    49501 atctaagcta tccacgaaca gacacccctt atattacaga aggagagcat gcgtatctgt
    49561 tggatcattt agatgaatac aagcattttt taaaggctga atcattccct actcctattc
    49621 atacaccgaa tagtcggtat gtgaataata agaaagtcca ggaacattac gcgatcattc
    49681 ctacaaaaac agttatgaca gaagcaacgt atgaaaagct gacaccttta caacaagcga
    49741 tttatgatca agttttaaag acgacggtgg ccatgtttgc ggaaaaatat acctatgaag
    49801 aaactacgat tttgacacaa gtccagcagc ttcagctaaa ggcagtcgga aaagtaccag
    49861 tagatctagg ttggaaaaag ctatttggca aagagagcga cagaaaagag aaggaagaag
    49921 aaccgctact tcctaaagta acaaaaggag aaacggtgac ggtagatcta caaatctttg
    49981 aaaaagaaac caagccacca caaccttaca cggaaggtac actaatcacc gccatgaaga
    50041 cagctggtaa aaccgtggat agtgaagaag cacaatccat attgaaagaa gtggagggaa
    50101 ttggtacaga agcgacacga gcaaatatta ttgaaacctt gaagcaaaag gaatatatca
    50161 aggtagagaa aaataaactt gtggtgacga ataaaggaat tttgctttgt caggctgttg
    50221 aaaaagaacc gctgttaaca agtgccgaaa tgaccgccaa atgggaaagc tatcttacaa
    50281 agattggtga gcaaaaaggg acacaagcta cttttctagc aaatatccaa aaatttgttt
    50341 cccacttgtt ggaagtcgtc ccaggacaaa tccaagccac tgattttgga gcaacgttac
    50401 aagaggtgaa agtagccagt gaaaagcaag aagcggcgcg tcatttagga tcttgtccaa
    50461 agtgccagga aaaagaagtc atactttatc agaaggtggc gacttgcacg tctgaagcct
    50521 gtgattttaa attgtgggta acgattgcca aaaagaaact cacagcgaca caactgaaag
    50581 agatcattca gaacggccga acaagtcaac cagtcagagg actaaaaggt cagaaaggtt
    50641 cgtttgaagc aacgatcgtt ttaaaagaag actttacaac aggatttgag ttttctgaaa
    50701 agaagaagac cacctacaaa agaaaagtga gacgtaaaac gagttagaat tgacggaggg
    50761 aaaaacgatg agaaatacaa cgcataaaga acaagaaatc gaaaatcaaa aagccaaaat
    50821 acgttcagag caggagaaaa tgcgtgccag caaacgaaga ctacagcaat tgaagaatca
    50881 acaacggatt caaaaagaac ttgaattaga tcgataaatc ctcctgttgt taatattaaa
    50941 ctaacatgtt atagttatgt tagtttaata ttaacattcg agagggaaag cgatgaaaga
    51001 taaagtcaag tgcatttttg agaaatacaa tggtaatcta tccttgaagg atgctagaaa
    51061 tgaagggtta tcacctatga cgttagatcg tttagccgaa aaggacaaaa tagaaaaaat
    51121 agcatcaggt ttttatacct taaaagatag ttttgtggat gaattatatc tcgctcaaag
    51181 tatctatact agaggaatct tctctcacga gacagcgctt gatctttttc aagtaggaac
    51241 atatatccct aaaaaaatta atatgatgtt tcctaaagga tacaatgttt cgaaagagaa
    51301 tttagatcag tatgccattc aaccgcatta cacgaataaa gaaaactttg aactgggaat
    51361 cacagaggca gaaagctttt acggcaataa aattttcgtc tatgatttag agcgaacgtt
    51421 atgtgatatg tggaatccca gatataaagc aagcgtagaa gtaaaacaag aagcattaaa
    51481 agaatatatg acaagtacca gcagaaatac aaataagcta agaagataca tgaatatttt
    51541 aaaatcacca aaagaaatga ctttgtatat gttgccactt tattgagagg agtaaaggtg
    51601 aatgacaccg acacaattta aagcgaaaac aagaaatgtt gcagctgaga aacatttaaa
    51661 cccacaaatt gttcaaagat attatatgat ggaaaaatta ttagaatgta ttgttgaatc
    51721 gccttatcat gatgattttg tattaaaagg gggatttctg atcggatcaa aatacgggtt
    51781 agaaaaccgt tcaaccgttg atattgatac gacttatcga aattcgaaac taacaaaaga
    51841 aaaattagaa actgtttttt ctgaattaac agctgctcct acaaaagatg gcattcggtt
    51901 tgaattaaag ggcttaacag agacgaggga agccgattat tacccaggtt ttcaagcaaa
    51961 gtttattgct tttcttgaaa atgcacgtgt accttttaaa ttagatatta cttcaggtga
    52021 ttctattatt ccagcagcat tgatatatga acataaattg atgtttgaag ataaaacaat
    52081 tcatattcca gcttatccta cggaacaaat tttagctgaa aaattacatg ccacgtttag
    52141 tttcggaata gataattcac gtatgaaaga tttctatgat ctgtatatga tccctaaact
    52201 agaacgtatc gatgagataa atttgtataa gagtgttcaa ggaacattta aggaaagaaa
    52261 taatcaatca gtgtttaaga atttgtataa tcaggaaatg cctcaattga tgaagaatga
    52321 gggattgagg gagaaatgga aaagatatca gcaagaaaac tattttgcca aagatatttc
    52381 tttcgaatct accttaactt ctgttgattc gctgatgcaa tcaattgtcg agcaagagga
    52441 agtagaacga aaacaaaatt ttaagagatc acaacaaatg aatcaagaaa tggagagata
    52501 aagatgatca ataatgtaac tttagtaggt cgattaacca aagatcccga tttacgttac
    52561 accgcaagtg gcactgcagt gaccactttt accttagccg ttaaccgtaa ttttactaat
    52621 caaagtggca atcgagaagc tgactttatc aattgtgtga tttggcgcaa accagctgaa
    52681 acgatagcga cattagctaa aaaaggcagc ttgatcggcg tcgtcggacg gattcaaacg
    52741 cggacttatg acaaccagca aggacagcgt gtctatgtga ctgaagttgt ggccgataat
    52801 ttccaactat tagaaagtaa agcagcgaca gaaagccgta tgcacgctga tcaatcgtcc
    52861 agcagtccaa gccctaccac ctttgaacaa cgggatacag cgacgcctaa caacaatgga
    52921 ttgaacgctt cacagaatcc atttggtgga caatcaatcg atattagcga tgatgatcta
    52981 ccgttctaag tagcattttc tattggttgc atttgctacg gtttatgtta taatttagac
    53041 aacaaaaaga attttcaatc tataaccaaa aaccactagc tggcgggcta gtggctttct
    53101 tttacgtttc tattataaga attaacgggt aggcttactg tctttcttgg caaatttaca
    53161 atcaagccat ttgcagatat agtgcgcggc tacttctgcc atgattgcaa aaacaaaatc
    53221 aaaaagaatt ttcaaaccta taacctcctc cccttgccag attaggccgg ggtagcagac
    53281 agcaagacta ttatagcata attgaaatat ccaaatgatt ggatttttgc tataatttta
    53341 gcgaggtgat tgtgatgaac aaatatgttg gtttgcttga taaaatccga gtaataaaaa
    53401 cgcaaccgtt attggttcgc tttactttac aaacgattca tgaatcgatt aattgtgtag
    53461 ttgcagatat tgaaattatc gataaattgc taattatgga tgatggaaaa tacaatattg
    53521 ccgtcacagg tcacttcaat aaacgaaatc agctagttat tgctagcatg tatgttagaa
    53581 atcccgatca ttttacgcga tcgatgggca tataaataaa agaagagttg tagtcctaaa
    53641 tgaggattgc agctcttttt gttttacata gaaacaggaa aagacctttt taggaagaca
    53701 caagtgcctt ctcaattgaa aaacctttct ctcactccaa ccgccaccgc cgttcgagaa
    53761 gaaaggaagt gaacgctgca cgttcactaa gatatggtgt cttgcaaagc aagacaaaat
    53821 agacaaaact tcctctcacc aaaaggtt
//
LOCUS       DACDKJ010000013        52293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDKJ010000013 DACDKJ010000000
VERSION     DACDKJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.013"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(124..501)
                     /locus_tag="IV328_000885"
     CDS             complement(124..501)
                     /locus_tag="IV328_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937045.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            757..1485
                     /locus_tag="IV328_000886"
     CDS             757..1485
                     /locus_tag="IV328_000886"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8937046.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1485..1739
                     /gene="purS"
                     /locus_tag="IV328_000887"
     CDS             1485..1739
                     /gene="purS"
                     /locus_tag="IV328_000887"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8937047.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1741..2412
                     /gene="purQ"
                     /locus_tag="IV328_000888"
     CDS             1741..2412
                     /gene="purQ"
                     /locus_tag="IV328_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8937048.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2413..4635
                     /gene="purL"
                     /locus_tag="IV328_000889"
     CDS             2413..4635
                     /gene="purL"
                     /locus_tag="IV328_000889"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8937049.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4620..6059
                     /locus_tag="IV328_000890"
     CDS             4620..6059
                     /locus_tag="IV328_000890"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8937050.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6091..7134
                     /locus_tag="IV328_000891"
     CDS             6091..7134
                     /locus_tag="IV328_000891"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8937051.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7131..7709
                     /gene="purN"
                     /locus_tag="IV328_000892"
     CDS             7131..7709
                     /gene="purN"
                     /locus_tag="IV328_000892"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8937052.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7706..9241
                     /gene="purH"
                     /locus_tag="IV328_000893"
     CDS             7706..9241
                     /gene="purH"
                     /locus_tag="IV328_000893"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8937053.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9257..10531
                     /gene="purD"
                     /locus_tag="IV328_000894"
     CDS             9257..10531
                     /gene="purD"
                     /locus_tag="IV328_000894"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8937054.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10653..11567
                     /locus_tag="IV328_000895"
     CDS             10653..11567
                     /locus_tag="IV328_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8937055.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11700..13265)
                     /locus_tag="IV328_000896"
     CDS             complement(11700..13265)
                     /locus_tag="IV328_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8937056.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13258..14709)
                     /locus_tag="IV328_000897"
     CDS             complement(13258..14709)
                     /locus_tag="IV328_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8937057.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14723..15739)
                     /locus_tag="IV328_000898"
     CDS             complement(14723..15739)
                     /locus_tag="IV328_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8937058.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15959..17047
                     /locus_tag="IV328_000899"
     CDS             15959..17047
                     /locus_tag="IV328_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8937059.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17101..18627
                     /locus_tag="IV328_000900"
     CDS             17101..18627
                     /locus_tag="IV328_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8937060.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18629..19834
                     /locus_tag="IV328_000901"
     CDS             18629..19834
                     /locus_tag="IV328_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8937061.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20091..21068
                     /gene="guaC"
                     /locus_tag="IV328_000902"
     CDS             20091..21068
                     /gene="guaC"
                     /locus_tag="IV328_000902"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8937062.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21332..22756
                     /locus_tag="IV328_000903"
     CDS             21332..22756
                     /locus_tag="IV328_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8937063.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23217..24326
                     /gene="pdhA"
                     /locus_tag="IV328_000904"
     CDS             23217..24326
                     /gene="pdhA"
                     /locus_tag="IV328_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8937064.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24329..25306
                     /locus_tag="IV328_000905"
     CDS             24329..25306
                     /locus_tag="IV328_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8937065.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25331..26974
                     /locus_tag="IV328_000906"
     CDS             25331..26974
                     /locus_tag="IV328_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8937066.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26982..28388
                     /gene="lpdA"
                     /locus_tag="IV328_000907"
     CDS             26982..28388
                     /gene="lpdA"
                     /locus_tag="IV328_000907"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8937067.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28550..28825
                     /locus_tag="IV328_000908"
     CDS             28550..28825
                     /locus_tag="IV328_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8937068.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28831..29598
                     /locus_tag="IV328_000909"
     CDS             28831..29598
                     /locus_tag="IV328_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8937069.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29766..31595
                     /gene="typA"
                     /locus_tag="IV328_000910"
     CDS             29766..31595
                     /gene="typA"
                     /locus_tag="IV328_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8937070.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31856..33628
                     /gene="recQ"
                     /locus_tag="IV328_000911"
     CDS             31856..33628
                     /gene="recQ"
                     /locus_tag="IV328_000911"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8937071.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33742..34044
                     /locus_tag="IV328_000912"
     CDS             33742..34044
                     /locus_tag="IV328_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8937072.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34047..35210
                     /locus_tag="IV328_000913"
     CDS             34047..35210
                     /locus_tag="IV328_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8937073.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35236..38664
                     /locus_tag="IV328_000914"
     CDS             35236..38664
                     /locus_tag="IV328_000914"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8937074.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38685..39809
                     /locus_tag="IV328_000915"
     CDS             38685..39809
                     /locus_tag="IV328_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937075.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39799..40236
                     /locus_tag="IV328_000916"
     CDS             39799..40236
                     /locus_tag="IV328_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8937076.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40220..40558
                     /locus_tag="IV328_000917"
     CDS             40220..40558
                     /locus_tag="IV328_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8937077.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40677..41237
                     /gene="rsmD"
                     /locus_tag="IV328_000918"
     CDS             40677..41237
                     /gene="rsmD"
                     /locus_tag="IV328_000918"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8937078.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41234..41725
                     /gene="coaD"
                     /locus_tag="IV328_000919"
     CDS             41234..41725
                     /gene="coaD"
                     /locus_tag="IV328_000919"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8937079.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41715..42758
                     /locus_tag="IV328_000920"
     CDS             41715..42758
                     /locus_tag="IV328_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8937080.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42917..43645
                     /locus_tag="IV328_000921"
     CDS             42917..43645
                     /locus_tag="IV328_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8937081.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43761..44006
                     /locus_tag="IV328_000922"
     CDS             43761..44006
                     /locus_tag="IV328_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8937082.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44003..44221
                     /locus_tag="IV328_000923"
     CDS             44003..44221
                     /locus_tag="IV328_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8937083.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44357..44896
                     /locus_tag="IV328_000924"
     CDS             44357..44896
                     /locus_tag="IV328_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8937084.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44889..47162
                     /locus_tag="IV328_000925"
     CDS             44889..47162
                     /locus_tag="IV328_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8937085.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47200..48246
                     /gene="holA"
                     /locus_tag="IV328_000926"
     CDS             47200..48246
                     /gene="holA"
                     /locus_tag="IV328_000926"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8937086.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48335..48589)
                     /locus_tag="IV328_000927"
     CDS             complement(48335..48589)
                     /locus_tag="IV328_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8937087.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48989..49258)
                     /locus_tag="IV328_000928"
     CDS             complement(48989..49258)
                     /locus_tag="IV328_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937088.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49728..50183)
                     /locus_tag="IV328_000929"
     CDS             complement(49728..50183)
                     /locus_tag="IV328_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8937089.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50321..52249)
                     /locus_tag="IV328_000930"
     CDS             complement(50321..52249)
                     /locus_tag="IV328_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8937090.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        17173 a         9000 c        11407 g        14713 t
ORIGIN      
        1 aagcagatcc gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag
       61 gaacctgatt ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag
      121 gaattaagcg taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag
      181 caagttttcg agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata
      241 atgatttttg aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc
      301 tgtctgataa agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat
      361 ttttcggaat gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag
      421 caaatcatta tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc
      481 ttgttttatt gcatcttcca tcggcaagtg cctcctcgaa cccattatcc aaatttttag
      541 ttaattctaa tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt
      601 tcgtttctga taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg
      661 ttgatttata aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt
      721 atttgctttt aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat
      781 gaaggaaaag ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat
      841 ttagatcaag caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa
      901 ttgaataatc aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat
      961 cattttattg aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct
     1021 ctggaagtgg ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc
     1081 gaaggtaaag aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat
     1141 gatccattta tcaatgaaga gcatatctat ttcttacaac ttgcaaaacc ggaagaaatc
     1201 agtgagctga aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag
     1261 gtcggtttgg ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg
     1321 cttgcggatg aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat
     1381 atggacaaag acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta
     1441 tataaccgtc tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag
     1501 tatatgtaac ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag
     1561 tccatcgtat gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac
     1621 aacttgctga tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc
     1681 ttgcaaatgt aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac
     1741 atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca
     1801 gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa
     1861 ggatttgatg gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt
     1921 gcgattgctc ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa
     1981 ccagtattcg gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt
     2041 gtactgagac gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac
     2101 cccaagacgg cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct
     2161 catggggaag gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat
     2221 cagattgcct ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt
     2281 acgaacaaag cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac
     2341 ttgctaggat cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag
     2401 gtgaccgtat agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga
     2461 atctatgccg aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta
     2521 ggaagaatgc ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt
     2581 tcctataaaa attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg
     2641 caaggccctg gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc
     2701 aaagcagaaa gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga
     2761 gttggcggaa tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat
     2821 tcattgaggt tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt
     2881 gcaggtatcg gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca
     2941 tttgatcctt gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa
     3001 cacaaacata tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt
     3061 gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa
     3121 gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg
     3181 cttgaagcgt gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat
     3241 atgggagcag caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga
     3301 cttatattga cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg
     3361 atgttatcgg aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa
     3421 atccaagaac tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac
     3481 gacgggcaat atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca
     3541 ttagctgaag atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa
     3601 tttcagcagt tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat
     3661 ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa
     3721 gtacaaacca atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg
     3781 acgaataaag cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca
     3841 aaaatcggag gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt
     3901 aagcccttgg cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt
     3961 tgggaattat cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct
     4021 gttatttcag gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca
     4081 ccaatgattg gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc
     4141 aaaaaatcag gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt
     4201 gaattacaaa agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc
     4261 attgaaaaga gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt
     4321 gctcatgact gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat
     4381 cagctaggga tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa
     4441 tcaagatttg tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga
     4501 gatgctgctg ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact
     4561 gacggtcaga tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta
     4621 tgtcttatga agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc
     4681 cagacgccgc tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag
     4741 gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc
     4801 tttctgaggt attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg
     4861 gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat
     4921 tcaaatttca tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt
     4981 cccttagaaa agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga
     5041 tattgatgca tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg
     5101 ctttgaatga agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg
     5161 ccgctttgga cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat
     5221 acgtgattgc ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg
     5281 tccagcctgg tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga
     5341 aggacacgca gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa
     5401 acatcgcagg gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag
     5461 aatcgccagt aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa
     5521 gcggttatgc ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca
     5581 ttgcccgaac atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat
     5641 tatctgctgt acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg
     5701 ttcggggtac gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag
     5761 tccatgtgag gattgcttct cctcctttga aatatccatg tttttacggg attgatattc
     5821 aaacgcgtaa agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg
     5881 gtgcagattc tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa
     5941 aagatgctcc gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt
     6001 tatatgacta tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa
     6061 agcagaccaa gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta
     6121 gatgtcgaag ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa
     6181 cgtttaggta tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt
     6241 caattaaaag agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg
     6301 gcaatcgaag cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat
     6361 gatatcttag cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa
     6421 aatgatccgt tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga
     6481 aatatggcgc tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat
     6541 tatgatctag ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa
     6601 acgatcaacc caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg
     6661 tactctcttg ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg
     6721 ccagaattga aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct
     6781 tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga
     6841 gggtttgtag aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt
     6901 ggctcatggc cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag
     6961 atggagatgt atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg
     7021 aaagtaaaag aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg
     7081 aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg
     7141 ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa
     7201 aaggattgga atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga
     7261 aacgtgcaac tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa
     7321 aaaaagagta tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg
     7381 tattagctgg atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa
     7441 tcatcaatat ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg
     7501 cctttgaagc tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag
     7561 atactggacc aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt
     7621 ctttagaaga aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa
     7681 ttattgaaaa tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac
     7741 tgggattgta gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg
     7801 aggaacaaag catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac
     7861 agggtttcct gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg
     7921 tcttttagga agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca
     7981 accaatcgac tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga
     8041 tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag
     8101 tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt
     8161 actttcagaa ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc
     8221 aaaagtcttt cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg
     8281 ggtggaagaa aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg
     8341 ttacggtgaa aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata
     8401 ttcgatcgca gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga
     8461 tgcagatgct gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa
     8521 acacatgaat ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc
     8581 gtatgaagca gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga
     8641 tctgccaaca gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta
     8701 tacacaagaa gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga
     8761 ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct
     8821 tgtacaagat caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg
     8881 taagccgact ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt
     8941 gaaatctaat gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca
     9001 gatgaatcga gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg
     9061 tgcagtttta gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa
     9121 acatggtata aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga
     9181 tatggcgaat aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta
     9241 gagaggagtc ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat
     9301 aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc
     9361 ggggatgaca aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt
     9421 aatccatttt gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt
     9481 gttaaatggg atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa
     9541 agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca
     9601 aatcccgaca gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa
     9661 agaaaatggt gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt
     9721 cgtggctacc acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg
     9781 atttggggat agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc
     9841 ccttttggcg tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa
     9901 gcgggcattt gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt
     9961 accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc
    10021 tttgggaatg aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc
    10081 tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca
    10141 agtcgttctt tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa
    10201 tcgtacagta gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc
    10261 tgccggttac cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga
    10321 gctttctgtt tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg
    10381 acgtctttat ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta
    10441 caccgcgtta atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa
    10501 agctgccaat agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca
    10561 acaaacataa tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact
    10621 tttgttgtgt atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg
    10681 atcagaaagc agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag
    10741 taaaagtgat tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt
    10801 atggaaacgg tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac
    10861 gagaagatct ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt
    10921 ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg
    10981 tggatgcttt acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag
    11041 cgtttgatga attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac
    11101 caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc
    11161 aatttggctt gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg
    11221 aagcaagtat cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga
    11281 tccaagcatg gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa
    11341 aattccctga attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa
    11401 ctggcttggc ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta
    11461 cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg
    11521 aagattggta cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag
    11581 caggtcaaaa gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg
    11641 agatattctg tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat
    11701 tagttatttt tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt
    11761 tttttcttaa ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata
    11821 tctgaaatac gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct
    11881 gtcagataat cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag
    11941 ggacttaatg caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt
    12001 tctttgataa aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt
    12061 gagggcagag cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg
    12121 tcggcgctga ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt
    12181 ttcttttctt tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat
    12241 cgtgttcgag ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg
    12301 tttttcgata atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga
    12361 agtttcgctt cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg
    12421 gtttcaagta tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg
    12481 acaggaccaa tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac
    12541 gtttgggcta cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa
    12601 aatttgatga atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa
    12661 aacttcaaat aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct
    12721 gctaataata cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc
    12781 aatgtttcac tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc
    12841 aattcgaaaa caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt
    12901 actgcttttt ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca
    12961 tctgcctgta cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat
    13021 atgagacgag tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg
    13081 aaaggcattt ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt
    13141 gccatctcac catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca
    13201 gttatttgtt cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta
    13261 tacatgtttg tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt
    13321 tccttctcgg tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag
    13381 gagacgcttg tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt
    13441 ggctaactct ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat
    13501 aactttgaat tggatcatct catcaatttt gcatgcggta atacgaataa atcctttgtg
    13561 acggacgtac ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt
    13621 taaaatagaa acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc
    13681 gtatcttatt tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg
    13741 aatccagtct tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat
    13801 agtcagagct tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt
    13861 ataaataaaa tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg
    13921 gtattgtttc aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat
    13981 gctagcggta agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt
    14041 ccctgaagaa agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct
    14101 ctctaaaaag cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc
    14161 cactattgtt aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc
    14221 gatctccaca cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac
    14281 ctgattgatg atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag
    14341 ctcgttttga taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat
    14401 ttctttaagt gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag
    14461 gatattttcc tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact
    14521 ttccaaaact ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat
    14581 cgtctgttga taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat
    14641 aacagcaaat agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt
    14701 ttttgtcatg atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct
    14761 gggaaaagac agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat
    14821 ttccaaaata acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga
    14881 ctacacaatt gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag
    14941 ttacaatgat caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac
    15001 cgtgagtcat ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt
    15061 ctaaatatcg tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt
    15121 gaacaatagt catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac
    15181 gattgattga ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac
    15241 cagcaagttc taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa
    15301 ataaacccgc ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct
    15361 cgggatcatt cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg
    15421 cttcttttaa tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca
    15481 ctttataggc aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat
    15541 ttcggtagag aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct
    15601 ttttccgcca gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg
    15661 cttcctttgt attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa
    15721 aaaataaaac acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca
    15781 gataaaaggg aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa
    15841 aaatccaaga caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa
    15901 tgttaacgct ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat
    15961 gaaaaacaaa tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt
    16021 aagtgcgtgt ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat
    16081 gccgaccaaa tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga
    16141 aaaactgggt tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc
    16201 acaaatagaa aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg
    16261 ttcggcttta actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta
    16321 tgatcgttta ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg
    16381 agtaggtgta tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg
    16441 accattcacg attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta
    16501 caacggagtg atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc
    16561 tggacagacg agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc
    16621 aaggatggat aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc
    16681 gccatatgat ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag
    16741 tgaaagtaaa cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc
    16801 gatcattgcc ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa
    16861 tacgattgaa atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga
    16921 aacttatgat aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga
    16981 taaagataat tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa
    17041 aaattaagag gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt
    17101 atggcggatt gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg
    17161 ttagattgcg tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac
    17221 ggagcgggaa aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat
    17281 gacggggaaa tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa
    17341 gaaaaaggca tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa
    17401 gagaatattt ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca
    17461 gaaaagaaaa catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta
    17521 gtatcccaga ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa
    17581 tccgtccgtc tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg
    17641 aatttgctga gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca
    17701 cataagctga atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag
    17761 acgattgaga cactagaaaa tgaaatgatc agcgaagagc atattattcg cgggatggtg
    17821 ggcagagatt tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt
    17881 aaagtagaag actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac
    17941 atcaacttgc atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc
    18001 agaactgaat tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt
    18061 atattaaagg aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga
    18121 ctcgcttatg tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg
    18181 gaaaacgtga cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa
    18241 aaagaagtaa ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc
    18301 catcaaattg tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg
    18361 ctgatgacac aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg
    18421 gcaaaatatg agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt
    18481 cttatctctt ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac
    18541 gaaggacgta ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa
    18601 ttaatgacga aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa
    18661 agagaaaaaa ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt
    18721 gatcatttta gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg
    18781 tccattgaat ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg
    18841 gatgttatta gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt
    18901 tgttggtgca attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt
    18961 tatcgcatgt cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta
    19021 tgcagggata ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac
    19081 acaagttgtt ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc
    19141 tacgggttat ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt
    19201 aggaatgatc atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa
    19261 aaatcattta tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc
    19321 agtcgttgta ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt
    19381 gatcattctt ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg
    19441 tcgacagatt tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac
    19501 aaagcgaatc acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt
    19561 agttttagct gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga
    19621 tgcaatggcg gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat
    19681 gggtgcgatt gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg
    19741 cgtcggggta gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct
    19801 agacttgtat aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag
    19861 actgtgacaa gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg
    19921 tttgtataga ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct
    19981 cttgattttt ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa
    20041 attaataatg ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat
    20101 ttgattacga agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat
    20161 gtgatacaac ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca
    20221 tgcagacgat tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata
    20281 tcatgcatcg gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg
    20341 gattgattac ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt
    20401 tggcagaaga atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta
    20461 actcagttat tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg
    20521 ccggtaatgt tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa
    20581 caaaagtcgg gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga
    20641 ctggaggctg gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca
    20701 tcgctgacgg tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga
    20761 caatggtcat gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag
    20821 tcgaaaatgg aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg
    20881 aaaaacgaaa tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata
    20941 cactagtgga aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc
    21001 tggaagccat tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca
    21061 caatctaaaa aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa
    21121 taagaacagc caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc
    21181 agatacaagc ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac
    21241 agtcccttat gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa
    21301 agaaaaaata gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg
    21361 accgatttat cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa
    21421 tcctggaagt caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc
    21481 caccgttgtc catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg
    21541 cagcggatgg tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg
    21601 gcggcaaatg atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga
    21661 agcaaagcag atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga
    21721 gcttttgccg aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa
    21781 agaaatggat cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag
    21841 taaatttgaa ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc
    21901 aatctttttg attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc
    21961 ttcttttttt taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc
    22021 gatggatgaa gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt
    22081 gaaagcagtg cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa
    22141 acgacgagaa aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga
    22201 tgtatttacg gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga
    22261 tccggataat gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat
    22321 cggttgggtg ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat
    22381 ggagttttcg atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat
    22441 tgatttgaaa atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc
    22501 ttggggagat tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg
    22561 gattacttgg gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg
    22621 tcttggaatt gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta
    22681 cagtaggctg gatgaaaacc aaataccgtt gttttattca aagatgaaaa ttctagaaaa
    22741 gtggttaaca gaataatttc acaaagtaaa tagattaaag aatataacag cttggattta
    22801 aatttcaagc tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa
    22861 accattattt gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg
    22921 tataacacca acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa
    22981 aaatgcagat gaaaaataaa acgctttcct tattgtgatt ttattcacta atgctgttaa
    23041 tatgcggatt ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct
    23101 cataaatcca tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact
    23161 gtttcaatcg cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg
    23221 caaacaaaaa agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc
    23281 cagtatatca agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt
    23341 tatccgatga tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc
    23401 aacgttcaac agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac
    23461 aagaggcaag tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag
    23521 gttatcggga tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat
    23581 ggtctcgagg acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac
    23641 aaattatcat cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac
    23701 gaaacaagaa aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt
    23761 tttacgaagc aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa
    23821 acaacgggta tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac
    23881 aaaaagctgt cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag
    23941 tatatactgt atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa
    24001 tcgaaacact gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct
    24061 accgtacaaa agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta
    24121 aatatttgac ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa
    24181 ctaaagaaga aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt
    24241 cagatttctt gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa
    24301 tttatgaagc gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac
    24361 agatgcctta gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt
    24421 tgggaaaaac ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga
    24481 ccgagtattc gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc
    24541 gttagaagga tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat
    24601 ggacgaagta gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct
    24661 acccatcaca attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga
    24721 taatttagaa ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc
    24781 ttatgatgca aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct
    24841 agaacacatg aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt
    24901 tcctttggat aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg
    24961 agcaatggtg cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt
    25021 tgaaatcatt gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt
    25081 ggaaaaaact ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc
    25141 gcaagtagtt tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg
    25201 agtttctgca cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa
    25261 tgcaaaagat atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg
    25321 aaacaaagtc atggcttatc aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg
    25381 tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact
    25441 agaagttcaa aatgacaaat cagtagaaga aatcccgtca cctgttacag gaacggttaa
    25501 aaatgtgatt gttccagaag gtaccgtagc aaatgttggg gatgtattag tagaaatcga
    25561 tgcaccagga cacgaagaca atgaagggga cagtggtgta gccgcagaat cccaaactcc
    25621 agctaaaccg gctgctgaac ccactgttga tacagaatca gctggatcgt caagcgaagg
    25681 agtcttccaa tttaagttac cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa
    25741 atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa
    25801 tgacaaatca gtagaagaaa ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt
    25861 tccagaaggc actgtagcaa atgttgggga tgtattagta gaaatcgatg ctcctggaca
    25921 caatagtgca ccgtcaacta gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac
    25981 atcaggttca gcaagtgtcg ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc
    26041 atctgttcgt caatttgccc gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg
    26101 aaaaggtggc agagtaacaa aagaggacat cgaaaacttc ttggcaggtg gtccaagcag
    26161 tgcaccagcg aaatcagaag cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc
    26221 agaaagcaaa ccagctgcac cagcaaaacc attcaagtcc aacttaggag acttggaaga
    26281 acgtgtagca atgacgccaa cacgtaaagc tatcgctaaa gcaatggtca acagtaagca
    26341 tacagctcca cacgtgacat tgcatgatga agtagaagtg tctaaactat gggataatcg
    26401 gaaacgattt aaagaagttg ctgctgcaaa tggtacgaaa cttactttct tgccatatgt
    26461 cgtgaaagct ttaacagcga ctgttaagaa atacccagta ttgaatgcat ctatcgacga
    26521 tgcaaaccaa gaaatcgttt ataaacatta ctacaatatc ggtattgcaa ctgatacgga
    26581 tcacggacta tatgtaccaa atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc
    26641 agacgaaatc aatgagaaag caaaattagc tcatgacggc aaactatctg cagaagatat
    26701 gcgcaatgga acaattacga tcagcaatat cggatctgta ggtggcggtt ggtttacacc
    26761 agtcatcaac tatccagaag tagctatttt aggtgtagga acgattgcac aacaaccaat
    26821 cgtaaatgct gaaggcgaaa tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga
    26881 tcaccgtatc gtagatggag caactgcgca acaagcaatg aataatatca aacgtctttt
    26941 agctgatcca gagctattaa tgatggaagg atgataaaca aatggtagtt ggagattttg
    27001 ctgtagaatt agatacagtt gtaattggtg cgggacctgg tggctatgta gctgccatcc
    27061 gcgctgctga aatgggacaa aaagtagcaa tcattgaaag agagtatatt ggcggtgttt
    27121 gtttgaatgt cggctgtatt ccttcaaaag cactgatagc tgcaggacac cattatcaag
    27181 agtcactaga ttctgaaatg tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa
    27241 aaacacaaga atggaaagaa aacaaagttg tccatacgtt gacttctggt gttggtttcc
    27301 tattaaaaaa acataaggtt gaaacaatcg aaggagaagc gttcttcgta gatgatcaca
    27361 cactacgcgt cattcatcca gattcagctc aaacttattc gttcaataac gcaatcatcg
    27421 caacaggttc ccgtccaatc gagattccag gatttaaatt cggaggacgt gtgttagatt
    27481 ctactggcgg attagcattg aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg
    27541 tgattggtgc cgaattgggc ggagcgtacg caaacttagg agcagaagta acgattcttg
    27601 aaggctcacc acaaatttta ccaacttacg aaaaagatct agtaaaatta gtagaagatg
    27661 atttcaagaa aaaaggcgta acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata
    27721 acggtgacag cgtgacagtg aaatatgcag tggacgggaa agaagaatcc gttactgctg
    27781 attatgtgat ggtcactgta ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag
    27841 cgggtgtcga agtaggagaa cgaggattga tcacggtgga taaacaagga cgtacgaacg
    27901 tacctaacat ctatgccatc ggtgatatcg ttccaggggc ggcattagca cacaaagcca
    27961 gttatgaagc aaaaatcgct gctgaagcaa tctctggcaa aaaagtagct gtagattata
    28021 aagcaatgcc ggcagtagcc ttcactgatc ctgaattggc ttctgtaggt atgacgatca
    28081 aagaagcaaa agatgctggt atcgaagcaa aagcatataa attcccattt tcagggaacg
    28141 gtcgggcatt atcgcttggt aaaacagaag gattcatccg tctagttacg acgatcgaag
    28201 ataacgtgct tattggcgcg caaatcggtg gagtcggcgc aagtgatatg gtctctgaat
    28261 tagctttagc aatcgaatca ggaatgaatg ctgaagatat cgccttgacg attcatcctc
    28321 atccatctct tggcgaaatc gtcatggatg cttcggaatt agcattaggt ttaccaatcc
    28381 atatttgatt tgaataatac aggcgaaacg caactgaaaa atcagttgcg ttttgttttt
    28441 tagaattatc aataatatta ttatgtaaac ttaaaaatat gacaacttct tggtttatta
    28501 ccacttccta tgctacactg aagaggaata tgaaatagga gagttgaaga tgcaagaata
    28561 tcagtatcca cttgatttag attggacaac agacgaaatg gtcattgtaa tgaagatgtg
    28621 ggaagatctg gaacgtgcga atgaaaaagg gataaacaat gaagcttttt tgaagactta
    28681 tcagcagttt aaaactgttg tcaaatccat tggtgaggaa aaacgattgg gacgggaatt
    28741 tgaaaaagct tccggttatt ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa
    28801 caaaactttg agaatgaacg ggtgagataa atggaacaga tgattgatca tatcaagtcc
    28861 tggcttgaag aagctggtga gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa
    28921 aaatcaaatc gaacggatct agtaacaaat gtcgatcgaa acatccagaa ttttctaatc
    28981 gaaaaaatcc attcctttga tccagaggcg aaaatattgg cagaagaaga agggtgtaat
    29041 caattatcgg atctcgatgg aagagtcttt atcatcgatc ctattgatgg caccttgaat
    29101 tttgtgattg aaggggaaaa tttttgtatt atgctcgctg tttatgaatc cggcgttgga
    29161 aagcttggtt tcatttatga cgtgatgaga aatgaacttt tttggggtgg aaaaggaata
    29221 ggcgtgtata aaaatgatga gccattgcct agaccagcaa ataaatcctt atcggaaggc
    29281 ttacttggtg tgaatagtta tcttttcggt cataataaat atggaatccg agaaattgcg
    29341 gaaagcagca tggggacacg tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg
    29401 aaaggaaacc acattgctta tatttcaaac cttagccctt gggattatgc agcgggaaat
    29461 gtcttattgg aagagtttgg tatggtaaca agcggtcttt ttggtactcc tttgacattt
    29521 gaaaaaaggg aatactattt agcagggact tcttgtgcat atgcagaaat cgcagacctt
    29581 ttgaaaaaag aaggctgaaa aaaaggtaga aatcattcac aaaaatgata aaaacgagca
    29641 aaagattaat tttctcgaaa aaatgaaaac attttactat atttcatgaa aaaagtctgc
    29701 tataattagc agtcgagtaa aatttgccct gtcataaaga accaataaaa aaggagttag
    29761 ttacattgaa ttatagaaac gatatccgta acgtcgcaat tattgcccac gttgaccacg
    29821 gaaaaacaac attagttgat gaactattaa aacaatcaga tacattagac gcacatacgc
    29881 aattgcaaga acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt atcacgattt
    29941 tagccaaaaa cacagctgta gactataaag gtattcgcgt aaacatcatg gatactccag
    30001 gacacgcgga cttcggtggc gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt
    30061 tggtcgtaga tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac
    30121 tggaacaaca tatcacacca atcgttgtcg taaacaagat tgacaaacct tctgctcgtc
    30181 ctgagcatgt cgtagatgaa gttcttgaac tattcatcga attaggagca gatgatgatc
    30241 agttagactt tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg
    30301 atccagcaga tcaagaacca accatggcac ctatttttga tacaattatt gaaaaaatcc
    30361 ctgctcctgt cgataacagc gacgaaccat tacaattcca agtttcatta cttgactaca
    30421 acgattatgt aggtcgtatc ggaatcggac gtgtatttag aggaacaatc aaagtaggag
    30481 accaagtagc attaattaaa ttagacggaa cagttaagaa attccgtgta acgaaattat
    30541 ttggtttctt tggactaaaa cgtctagaga ttcaagaagc aaaagctgga gacttgattg
    30601 ctgtctcagg tatggaagat atctttgtcg gtgaaacagt tactcctgtg gatcatcaag
    30661 atgcgttacc tatccttcac attgacgaac caacattgca aatgactttc ttagttaata
    30721 attcaccatt tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt
    30781 tgatggcaga acttcaaaca gatgtatcct tacgagttga accaacaaac tctccagatg
    30841 catggacagt ttctggtcgt ggagaattgc atttgtctat cttgattgaa aacatgcgcc
    30901 gtgagggata cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg
    30961 taaaatgcga accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg
    31021 ttatcgagtc cttgagctta cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg
    31081 gacaaatccg tttgacattc ttaacacctg cccgcggttt gattggttat tcaactgaat
    31141 tcctttcaat gacacgtggt tacggaatca tgaaccatac ttttgaccaa tatctgccaa
    31201 tgcttcctgg tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa
    31261 aagcaacgac ttattctatc atgagtatcg aagaacgcgg tacagtcttc gtagaaccag
    31321 gtacagaagt atatgaagga atgattatcg gggaaaacag ccgtgataat gatttgactg
    31381 tcaacatcac gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa gaccaaacat
    31441 ctgtcatcaa aaaaccaaaa caattgacgt tggaagaatc tttggaattc ttgaatgatg
    31501 atgaatattg cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa
    31561 atgctcgtga aaaagcatct aaaaagaaaa aataatctgt aaaaggatga agagagaaac
    31621 cagcttgtta taaaaaatag ctggtttctt tttttgtcca actcttttct gtggagaagt
    31681 catctttgtc aatggacata aattcagaaa atacgccgaa agacgtttaa aagactttta
    31741 aaaaacttat ttctagagag tttttgtttc ataataagat aatgtttcac gaaaggagat
    31801 caacttgtcc ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa
    31861 cgacttatta aaacaatact ttggttatga tgagtttaga cctggtcaaa aagaaattat
    31921 ccagaaagtc attgatcagg aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc
    31981 aatctgttac cagcttccag ctttactatt agatgggctt actgtggttg tgtccccttt
    32041 aatctctttg atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt
    32101 tatcaatagc tcgttagatg gttacgaaac agctagacgc ttccaagaaa tcgatagaca
    32161 gcagtaccgt ttactttata tcgctcctga acgttttatc atgccggatt ttatccaagc
    32221 aatgaagcga tggaacgtcc gtatgatcgc tattgacgag gcacattgta tctctcagtg
    32281 ggggcatgat ttccgcccaa gttatttaca gatggcgaac cagctagatc aactgccaaa
    32341 tcgtcctgta attgttgctt tgacagctac agcgactgtg caagtagctg ctgacataaa
    32401 aagactttta aaaattccag aaaacaacca tattcagact ggttttgaac gagaaaatct
    32461 acgatttcaa gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat atttaaagat
    32521 caacaaaaat caatctggta tcatttatgc agcaacgaga aaagaagtag accgtctcta
    32581 tcatttgctt aaaaaattcg atttttccgt gggtagatat catggtggtc taaatgagaa
    32641 tgagcgaaca gaaatgcaag aagccttttt atacgatcgc ttgcagctga ttgtagcgac
    32701 aaatgcattt ggtatgggta taaataaaag caatgtccgt tttgttatcc attatcaaat
    32761 tcctggctct ctagaagcat actatcagga agctggtcgg gcgggaagag atggtctttc
    32821 cagtgaagcc atcctgcttt tctctcctca agatatccaa gtccaaaagt tttttatcca
    32881 gcagtcccaa agagaagaag ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac
    32941 agaatacgtg tatattgagt cctgtcttca gcaatacatc ctaaactatt ttggcgagac
    33001 aagcagtccg tgcaaccgtt gtggtaactg tttagatgac agagaaatag tagaagtaac
    33061 gacagaagca cagatggttc tttcttgctt aaaacgtatg ggtgagaact atggtaaaca
    33121 aatgctgatg aaagtcttag caggatcgaa agaacaaaag ctgcaagctt taggttttgg
    33181 acatttatct acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat
    33241 tgaatatttg atatcgaatg gctatcttct gaccatcaat ggtgagtatc ctgttttgaa
    33301 agtaacagaa agagggatac aagtattgaa aggacaagaa tctgtttatc gaaaggaacc
    33361 caagaaagtc cagcagctat cggatgaaga aaccgataca ctatttgaag tgctgcggga
    33421 actgcgaacg gatctagcat cagaagcagg cgttcccccg tatgtcgtat tttcagattc
    33481 cacattgaaa gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc ttcagataaa
    33541 aggagttggc caaagcaagc ttgataaata tggcgaagcc tttttatctc ggataaaaaa
    33601 tgcggactct aacgttctgg ataagtagaa agaacgaata cgtaaaaatg aataaaaaca
    33661 actggttaaa tctgtttttt ttgctcctta tgaaaatcaa atatgttata attaaggaat
    33721 tagaatggga ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat ccaagtatta
    33781 aatgatgaag cagaacggat caaacgatta atccgaaatc aaaacaacag tctctgtatc
    33841 tcacaatgca aagccttcga agaagtggta gatacacaga tgtacggctt ttcccggcaa
    33901 gtcggttttg ctgtccgtat aggagtaatc ggaaagaacg agggccagct gatgttaagt
    33961 gagctagaac gagaattgaa tcagttatat agtgaggttt accaagaaat ttatgataaa
    34021 aaagaagttg gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg catctattag
    34081 actatagcat cttgattccc tatttaattt tatgtgtatt gggattgatc atggtatata
    34141 gttctacgtc gtatctatta ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc
    34201 agagtatttt ttgggtgctt agcttgattg ctatcgcact actttataaa atgaaaacag
    34261 atgttttgaa aaaccagcgt ttgatcatgg cagccatcgc agttttgacg attttgctgc
    34321 tgattgttgt gttttttggt aaggagatca atggggcgaa aggttggctg caaattgcag
    34381 gattctcgat ccaacctgcc gaatacctaa aaatcatttc tatctggtac ctgtctctga
    34441 ctttatctaa aagacaaaac agtgtacaaa aagattttct tgggactgtg aaacgtccac
    34501 ttgcaatggt tatcggactt actgcattag tcgctattct tcccgatttt ggtaatgcgg
    34561 cagttatctt tttgattatt ttggttctgc ttttagccag cggagtcaat tatgtgtata
    34621 ctttgattgt aggagtagga ggcttttgtc taagtacttt tacgatctgg ctgatcaata
    34681 tcacaaacgg aaagatctta cccggacgtt tacagtatat ctataatcga tttgctattt
    34741 accaaaatcc tttctcagac gaattaaata aagggcatca actagtcaac ggctactatg
    34801 ccatgttcaa cggaggacta tttggcaggg gattaggtaa cagtatccag aaaaaaggat
    34861 ttttacaaga agcacaaacc gattttattt atgcaatcgt agtggaagaa ctgggagtga
    34921 ttatggggat tttgatatta gcgttgctat tctttatgat tgtgcgcatt gtccttgtag
    34981 gtatccgttc aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt
    35041 tgatccaagt atttgtcaat cttggaggaa ttactggcgt tattcctttg acagggatca
    35101 cgttcccatt tttaagtcaa ggaggctcca gtttgctgat gctttcgatt tgtgtcggct
    35161 ttgtcctcaa catcagtgcg gatgaaaaac gcaagagctt aggtttataa taaaaaaaga
    35221 tgtaggagtg gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt
    35281 gtatttcgtg cttgtacaga attaggaatt aaaactgttg gaatctatgc agaagaagat
    35341 gaatattccg tacaccgttt caaagcggat gaagcatatt tagtaggaca aggaaaaaaa
    35401 ccgattgatg cgtatctaga tattgaaggg atcatttcta tcgcgaaaga atgcggagct
    35461 gacgctattc atccgggtta cggtcttttg tcggaaaact tgaattttgc gcaacggtgc
    35521 gaagaagaag gcatcatttt tgttggaccc aaactgcatc atttagatat ttttggagat
    35581 aaaatcaaag caaaagctgc agctatcgaa gcggggattg cttcgattcc aggcacagat
    35641 ggaccaatcg cttctataga cgatgccttg gagtttgcga aacaatacgg ttatccgatt
    35701 atgatcaagg cagctctagg cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag
    35761 agtgcaagag agggctacga acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat
    35821 gaagtgtatg ttgaaaaata catcgctaat cctaagcata tcgaagtaca gattctcggg
    35881 gatacgcatg gaaacgtcat ccatcttttt gaaagagatt gttctgttca gcgccgtcac
    35941 caaaaagtag tagaagtagc gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc
    36001 tgccaggcag ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa
    36061 tttcttgttg aaggagatga tttttacttt atcgaagtca accctcgcgt ccaagtggaa
    36121 catacgatca cagaaatgat tacagatatt gatatcgtaa caacgcaact gttgattgct
    36181 caaggtttgg atctgcataa agaaatcgga ttgcctcagc aagaaggaat taaattaaat
    36241 ggttcagcca ttcaatgccg aatcactaca gaagacccgt tgaataactt tttaccagac
    36301 acagggaaaa tcgatacgta tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga
    36361 aatgcttacg cgggctatgt agtgacaccg tattttgatt cattgctagt aaaagtatgt
    36421 acgcatggtg ctacctttga aacagccatt cagaaaatgg aacgttgctt gaaagaattt
    36481 agaatccgtg gagtgaaaac aaacattcca tttatgctca atgtcatcac acatcctgaa
    36541 ttccaatcag gaaatgcaaa aacaaccttc attgatagta cagcaacatt atttgaattt
    36601 cctcgactac gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat tactgtgaat
    36661 ggttttccag gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa
    36721 gatctaatca caagaactga ttacgtaacg gctaagaatg tattagatgc gaagggtgcc
    36781 gatgctttgg tcgaatggat caaaggacag gaaaatttgt tgttgacgga tacgacattt
    36841 agagacgccc atcaaagtct gctagcaact cgtgttcgga caaaggattt caagcagatt
    36901 gcacgtttga ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca
    36961 acatttgatg tggcttaccg ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt
    37021 cgttctttaa tgcctaatac gttgctgcaa atgcttttca gaggttcaaa tgccgttggg
    37081 tattctaatt atcctgacaa tgtattggtg gaatttgtca aagaagcagc agcgcaagga
    37141 atcgatgtat ttcgaatctt tgatagtttg aactggactc cacaaatgga aaaaagtatc
    37201 caagctgtac gggatactgg taaaatcgca gaggcagcta tttgctatac tggcgatatc
    37261 aacgatccaa gtcgggcaaa atacaatgtt cagtattata aagacatggc aaaggaacta
    37321 gaacaactgg gtgcgcatat cattgcaatc aaagatatgg ctgggctgct taaaccgcag
    37381 gcagcttatc gtttgattag tgaattgaaa gaaacgacag atctgccaat ccatctgcat
    37441 actcatgata ccagcgggaa tggaatcatc acttattctg ctgcttcaaa agcaggagta
    37501 gacatcgttg acgtggcaat gagtgcaatg agtgggaata ctagtcagcc tagcatgagc
    37561 agcttgtatt atgcactggt caatggtcca cggctgccag aaatcacgat tgaaaatgca
    37621 cagaaattaa accattattg ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc
    37681 ttgaatgctc ctgaaacaga agtatatatg catgaaatgc caggaggaca gtattcgaat
    37741 ctacagcaac aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat caaacaaatg
    37801 tatcatactg tcaacttgat gtttggcgat atcgttaaag taactccatc atctaaagta
    37861 gttggagata tggcactatt tatggtccaa aatgatctta cagaagaaga tatttatgaa
    37921 aaaggcgaaa cactaagttt ccctgaatct gttgtgactt tcttccaagg agaactaggg
    37981 caacccgttg gcggatttcc taaaaaattg caaaagatta ttcttaaagg cagacctgca
    38041 ttgaatgaac gtccaggtct tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa
    38101 ttggcagaaa aaattggcta tgaaccaaaa caagacgaag tattaagcta tttgatgtat
    38161 ccgcaagtct ttttggacta tcaaaaagca tataatcaat ttggcgatgt tacattactg
    38221 gacacaccaa catttttcca aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa
    38281 cgcggaaaaa cgcttatcat tcgtttagat gaaatcggag aacccgatat cgaagggaat
    38341 cgcgtactgt tcttcaattt gaatggacaa agacgtgaaa tcactatcaa agataattcg
    38401 atcatcagta cggtccaaac ccgattgaaa gctgaaccga caaaccgaga acaaattgga
    38461 gcaacgatgt ctggttctgt tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa
    38521 ggtgatacgt tgatgattac tgaagcgatg aaaatggaaa cggccatcga agcacgtttt
    38581 gacggagaag tcgcacatgt atatgtgtct tcaggagaca ctatttcgtc tggtgacctt
    38641 ttgattgaag tgactgagaa ataataagcg aaaggtgggg aaacatgaaa agactgggtt
    38701 tgttttggct gattctgcta ttagttattg tggcattcta catggaacca gtcttgtttc
    38761 cgccaaaact tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa atgacaacgt
    38821 ggaaatacca ggagcttaaa gcaactggtt tttctactta tatcggaaaa ccgatcgatc
    38881 agctggaaaa ggattttggc caagcctatg atcgattaac tagcggattt ggttttgaaa
    38941 caagatatta tgaaaataaa gctagtcatc tcacatttga agcgaacatc caagacagca
    39001 gagtcacaac tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg ttttatttag
    39061 ggatgacgat gcaagatctt acgaaaatca ctactatcta tactaatttc acttttgagt
    39121 accatgatac agaagttgga atcgaactaa tggaagagga tatgaattat cgtccgctaa
    39181 ttgcttttga taacgacact tttgctattt tattttttga tcaaacaaca aacggcctat
    39241 tttcagttgt gtatttagat aaagatagtc ttttgaaact attgccatat caagtgtttg
    39301 gtgaaggcct tccacattat caagcagata aatcggctga ttgggaatca atcaatcgcg
    39361 ttaaagaacg aaaaagtacg actttattaa atatgcttag aagagaagac gaacttccca
    39421 tatacaatca aacacttgcc tttcaaaacc acagtcagct gctactacat gattttttga
    39481 caaggcctga ggaaatattg tcagaggaac gtatggctga ttggcaaaaa tcgctaggca
    39541 gtgctcaaac gacagtgaaa ttctcactga cgaatgatga attggagaag ttaatcagta
    39601 aacaaaaatt agaaaatact agtgggctat ttattcatcc ggcgattgac gctacttttt
    39661 cgtttttata ttggtacagc gatccttaca accatgaccg tttcatggac ccgggaacgg
    39721 attctgtagg cattgctttt tctaaagaaa atatgttagt cttattgcaa gaagaaaacg
    39781 gagaaagtag tgatagcagt gatcgttaac gaagagctgt ttcaactgga ggatcaatgc
    39841 ctccaagtcg ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct tgaacataaa
    39901 cagtcaatgg aagaatctgc aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa
    39961 gaagcatttg aaaatgtagc ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa
    40021 cgagctgttc gaaaagcaaa aagacagctt gatatggatg agcatgttgc agctttccga
    40081 tatagtgaaa cacagctgca aagtctgttg gatcaaattg tccaagtact tgccgaagaa
    40141 gtttctaatg aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt
    40201 ggatgcggag gtaattgtca tgcaattaga caatagtgaa tttgaaataa caaaacgacg
    40261 cgggctgatc gtatgggtct acagcttaaa acaattaaag aatctgaaaa agttcggctt
    40321 gttgcattat gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg aagagacatt
    40381 ggaagaaaca gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag aacgttctta
    40441 ccgaccggat atcgaaatga attttgccga aaaaatagga aacaaaacag tattaaaaga
    40501 cgaaggtttc gaaattgaag agctaaatac agaaatccga ttagctgaac atctttaaaa
    40561 atctcttatg agcagttttt aaataaaaaa taacttggat aacgatcgat ttttgatcat
    40621 tgtccaagta tttttttggt atactagtac cagtaccatt tctaaggagt gagtagatgc
    40681 ggataatatc aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca
    40741 gaccaactac agataaagta aaagaatcca tattcaatat gattggacca tattttgatg
    40801 gagagacagt attggatctg tttgcaggaa gcggtggttt agctatcgaa gcagtatcta
    40861 gaggctgcag tcatgcggta tgcgtagaca aaaattacca agcgctaaaa atcatcaaag
    40921 aaaacatcga aattacgaaa gaaccagaga aatttaccac aataaaactt gatgcggaca
    40981 aagcaatcga acggctagca gcggagaaac aacaattcga ctacttgttt cttgatccgc
    41041 catatgcaaa acaaaaaata atcgatcaaa tccataaaat ggaagaactg ggactttttt
    41101 ctaaaaccgc agttatcgta tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg
    41161 ctggattcca tcaaataaga aaacagattt atggcataac ggccgtaact atctatagaa
    41221 aagagggaaa tcaatgaaac gtgcattatt cccaggaagt tttgatcctt ttacaaaagg
    41281 tcacttagat acggtggaac gtgcggctaa attattcgat gaggtagtaa ttggtgtttt
    41341 tatcaatact tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat tgatcacaaa
    41401 agctgtttct catcttccta atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga
    41461 aacagcaaaa gagattggtg cagatgcgtt gatcagaggc atacgaagta tcaaagactt
    41521 tgaatatgaa cgagagattg ctcagatgaa tcatcatcta tatgatgaat tggaaactgt
    41581 cttcttatta gcaaaaccag aatatagtca cgtgagttcc agtattttaa aagaagtact
    41641 gcactttggc ggagatgtga gcagctactt accgccagtg atcaacgaag ctttagctgg
    41701 aaagcgtgaa tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt atcatatttg
    41761 gaatgttgat cattggattt gtagtagttc ccttgccgta ttatgtagaa tcaccgggag
    41821 ccacgatcga tctgaaagaa ctaattacgg tcaatgataa ggaagataaa ggttctgggt
    41881 ctttctctct tacctctgtt ggtatcaggc aagctacagg tttcttagct ttaaaagcta
    41941 aaatgacccc ttttgaagaa ttgatcagtg aagaagaatt gactggtggg gcgacaaatg
    42001 aagaatataa tcaaatgcaa cagtattata tggattcttc ccaaaatgca gcaatcgaac
    42061 aagcgttaaa acttgcagac aagccttata aaatggaatt caaaggcgta tatgtcatga
    42121 gtgtagagaa aacatcgaat ttctttggca agctttcagt tggagataca gtaacaaaag
    42181 tagacgggaa aagctttcaa agtacagaag catttatgga ttatgtaaaa agccaaaaag
    42241 ttggacaaac aatcgaaatc agttatttgc ataatggtga agaaaaaaca gcttctggtg
    42301 atctgatcga gttaccaaca gataaaaagg caggaatagg tatcacgctt actgaccata
    42361 ccgagatcga atcagataca gatgttcgta ttaattctgg ctctatcggt gggccatcag
    42421 cgggattgat gttcacttta gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg
    42481 gtaagcaaat tgcaggaact ggaacgatca acagtgaagg agaagttggc cgaatcggag
    42541 gaatcgataa aaaagtggca agtgcggata aagctggcgt agagattttc tttgcaccag
    42601 atgatacgat ttcagacgat gttaagaaag aatatcctga aataaaaacc aactacgaag
    42661 aagcaaaagc tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag
    42721 tacaagaagc gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt
    42781 tgtgacacga gttctgtcac aacccctctt tttcgtcaaa aatcttcacg ataaaaataa
    42841 aaacaaaaga aaaaattgac gtaattagat gttgaaggga cgattttgcc agagctcact
    42901 gtttcttcaa ttttaaatga aaggagtaat gaatgatttt tactggtcat tctttgaaaa
    42961 aagaacaatg atagaaaaaa tcagcaagaa aatactagct tcacccaaat tgatcatttg
    43021 gatgtctatg atagttctta tcttttgtat cgcaagtggt tttttattat caaatttgct
    43081 attttataga gaaacagaag agactgctgc aaaagaaata ctttcttcgt ttagttccag
    43141 tggagaagat ccatcagctg aaggaaacga tgtagaagag gatgctcgtc caaaggtcat
    43201 gtacaccgat atcaaaggaa gtgtgaagga gcctggtatc tattcctttt cttcagaaga
    43261 acgggtctat gatgtactaa agcgtgcagg tggattatta gaagaagcgg atagcgatcg
    43321 aatcaacttt tctgcgaaga tagaagatca gcaagtccta tatatccctg ctgtaggtga
    43381 agagctgcca gaacatcttg atcagttggc atcctcggaa ggaaaacagc ctacagcaga
    43441 ttccgagcct cccaaaataa atatcaatac agccactcca tcagaacttc agcagattcc
    43501 tgggatcgga tcagtcaaag cgcaagaaat cattcgattt agagaagaaa acggatcctt
    43561 ccagaaagtg gaagacctgc aggagatttc tggtatcggt gaaaagacag ttgaaaaact
    43621 aaaaaacttc gttacaatta aatagaatga ttcaaaatcg actggcacga gcaataaatg
    43681 gacgctaatg gatactaaga ataatccaat tctaagagct tggcaggacg aggaggtagt
    43741 atttttggat aggaaatagc ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga
    43801 atgtctatct ccaaaataaa atatctggga ttcatattat gaatacggct gtcagcggca
    43861 ataacagtca agctctacga gaacgctttg ccaaagatgt acttagctat acagcagata
    43921 aagtatttat tctcattggg acgaatgatc tagcagagca taaacaactt tctaaagaaa
    43981 cgtaccaaaa aatctgcagt ggatgatagt tcagctgaaa aaagactata gttctgatca
    44041 tgctttcttt atcacgccac cagcagtcga tgagacaaaa caaaaatatc ggaataacaa
    44101 gttgatcaaa gaatatatct ctgttctcac agaagtagca aaacgccatg attgtgaagt
    44161 atcgattttt atacggaatt tcttgaacaa atggattcga agaattttta taatgggcta
    44221 atggatgacg gacttcattt cggcagtgaa gggtaggatc ttttgtcaga caggatgatt
    44281 cgttatattt aaagaatatt ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa
    44341 cggaggaata taaaacatgt tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa
    44401 agttactgat aagtcacata atgaacgcat tccttgggac cagtacttta tggctcaggc
    44461 agtactgtta tctttacgta gtacgtgtac acgtttagaa gtaggagcga cactcgttcg
    44521 agaaaaaagg atcattgctg gcggctataa tggagcagtc agtggagatg tccactgcat
    44581 agatgaaggg tgttacgtcg tggatggaca ctgcttgcga acgatccatg cagagatgaa
    44641 tgcactccta cagtgtgcaa aacttggtat cccgacggaa ggttcagaaa tctatgtcac
    44701 acattttcca tgtttggcct gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa
    44761 ttatctccat gactatcgaa atgatcctta tgcccaagcg ttgatcgaac aattaggtgt
    44821 atctgtacac caagttgctt tagactcaca gcatttttca aaacttcaaa atgaattaac
    44881 aggaaaacat gtctagcgtt ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa
    44941 ttacgtcgat gattatctgt ttgttgtatt acactgacaa ttggggttat cgtttgcttc
    45001 ttgccggaat ttttttatgg ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta
    45061 ttttgtgtag cataacggta tccatttctt ataggctaca agagtattca tttttagagg
    45121 aaaaaacaac atctgcgcaa attactgtat ttacagatac gattcgtgca aatggtaatt
    45181 ttttgacaat ggaaggaagg ttatcagaga ataaacaaaa gctccagctt tcttatcaag
    45241 taaacacaga agcagaattg aaaacatggc ttaacttccg aggaaaggga atagaattga
    45301 ctgctgaagg cgagttcatc tctccagcaa aaaagcggaa tctttataat tttgaccaag
    45361 aagcttactt caggaaccat agaatttctg gacggttcca aattcaaaag attgaacaga
    45421 tcaccatgca aaatcactgg cgaaactggc ttcagagaaa aagaggatct tttatctcct
    45481 ttgtccaatc tgaatttccg caaaaaacaa gtgtatatat taattcttta ttatggggct
    45541 ataaagatgc aggattcaga gaaccagaag aaatttttag ggagagtggt ttgctgcatc
    45601 tgttcagtct atcgggtcta cacatccaat tttatttagg gtggctgtat tatttatttc
    45661 gacgtatcgg atggccacta cacgtcagta ttattccgtt gagtctacta actatctgtt
    45721 atgtttctat tgcaggaagt tctatttctg ttttgcgtgc tagtttgctg tttttattgc
    45781 gattgctagt caaattgctg aataagcatt attccaccat ggacttattt gcagttgtct
    45841 tttggcttct tttactattt gatcctcgat ttctttttca tgcgggtgga cagcttagtt
    45901 ttttcatgag ttttttgttt atcctgattt cttttgatgg agattggttg aaaaaaacgg
    45961 cctctcacgt cctgtttact atgatcacga tgccgttgat tgtatggcat ttttatgagt
    46021 ggcctatttt aggtagtctt tttacattac tgattgcacc ggtattcaag tttctttttc
    46081 ttcctggtgt gttatttctc acactcttca atcgctggct gccggaaact ttacttttgc
    46141 tgatagaaga gggattaatc ttatttgagc gaataatcga tttttcttca gggtcgtctc
    46201 tcattatagg acgtttgccc cttgtgctat ctgggatgta tattttcgga ttactgattt
    46261 gtatggatag gttgaaagaa caacctgtga aacacttgtt ttatgttggt cttttttctt
    46321 ttattttttt gactttgcca tttttacgct tttcttcaac gatcgctttt atcgatgtcg
    46381 gtcaaggaga cagtatcttt ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata
    46441 ctggaggtca gttacacttt gaaagagaaa agtgggcgca aggtatgata cgtcctcctg
    46501 ctgaaagaaa tatcgttcca tatctaaaag gtcaggggat aagtgtaatc gataaactca
    46561 tcttgacaca cgacgatacg gatcatgtgg gagaattacc cacgttatct cagcatttta
    46621 ctatcaaaaa aatttatatc ggttggggcg ctggacgaaa tgagccatta caaaggcatt
    46681 tatctgaagc tcaaaaaaat ggtactgaga tcattgaaat caaacatggg gacaggatca
    46741 gcggttatta tgatctttat gtattgactc cttttgaaaa gggagaggga agaaatgaag
    46801 attcgattgg tctatggatg gaatataaca ataggcgctt tttattttta ggagatttga
    46861 atcaagcaat ggaaaaacaa ttgcttttga tttatcctaa tctgaaagcc gatgtagtca
    46921 aattaggtca tcacggtagc cgtacgtcaa gtaacacaga ttttctttcc catattgaag
    46981 caaaacatgg gattatatcc tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag
    47041 tattggagac actagaagaa aatcagataa aaacatggcg aacggatcaa caaggaatga
    47101 tcacctatcg ctggcatcca gtttttcatc ctcatgggct agtggaaaca atgatagact
    47161 agtaattctc atctgctcat ggtaggataa tttttgaaag tggtgagtaa tttgagtata
    47221 caagcagagt tacaaaaaat acgtaaagaa aaacttgcat cttgttactt gattttagga
    47281 acagaaaagt ttctccaaga tcaagttcgg acagaaattg tcaaaaaaat ccaattagag
    47341 ggcgaagatg acttaaattt tttaagtttt gacatggaaa atgcaagttt agatgaggta
    47401 gtagctgaag cagagacgtt gccgtttttc ggagatcaac ggttagtatt cgtagagaat
    47461 ccttattttt taacaggtga aaaagtaaac aatggaatcg aacaaaatac tgatttattg
    47521 acggcttatt tgaaagaacc attagaatcg actgtattag tattctttgc gccatatgaa
    47581 aagttggatg aacggaaaaa agtaacgaaa caattgaaaa aaacagcaat tatcatcaat
    47641 gtccaacagt tgaatgaaaa agaagtacgt caatatctga tgaatacctt agagaattcc
    47701 tcaatcaaaa tggaacgaag tgcaattgat ttattcttga ggttgaccga cttagattta
    47761 tccaaaatga tgagagagtt acagaaattg gtcctttatg gacaaaatca acagcaaatc
    47821 acggtaagag aagtagaaga attagttcct aaaacgttag aacataatat ttttgacatg
    47881 acccaatata ttttaaaagg gaagacggaa caagctttgc gtttatacga agatttagtt
    47941 atgcaagggg aagaaacaat caaaattaat gcgattttgc tttctcaatt acgtttattt
    48001 ttacagacaa aatttcttgt aaagattggt tatcagcagg caaatattgc tgaaacattg
    48061 aaaatccatc catatcgtgt aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat
    48121 ttgctggtac agttgtttga tcaattagtt gagatggatt atcaaatcaa aacgggacaa
    48181 atcgaaaaag aattaagttt tcaactattc gttttgcgca cagggcagtt gttaagaaat
    48241 cgataagaag aaagacgagg gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt
    48301 tgacaaataa aaaaatcggc cgaagccgat tcttttattt agcgattttt ttgcttaaac
    48361 gagatttgtc gcggttagct ttgtttttgt ggattagacc ttttgattca gccatatcaa
    48421 tagctttaac agcttcttta tataacgcat ctacgttgtc agcacctgaa gctacagcat
    48481 cttcaaattt tttgattgca gtacgcatag cgcttgtttg agatgaattt ttcacattag
    48541 cgttttcgct tgtacgaaca cgtttgattg cagattcaat gtttggcatt agtttcacct
    48601 ccgataactt aagttcttac gcgtgttacc gcataattca acattcatta ttatacctaa
    48661 aggcagcgtg ctttgcaata tgaacatgac aagttttttt ccttaacagt cttttattag
    48721 gatttttttt cagtaaaaac ggatagtaca agctgaaaaa aatcaaaaag acgctattgt
    48781 gggaggcaat agcgtcaaac caaactagat ttttatttat ctatttaaac aaagtttgaa
    48841 tgggaatgtt tggtactaag ttattatata acaaagagaa ccgtttgcaa agtttttttt
    48901 gaaaagggat acgattccct gttgttcttt gtttttctat taagaaactg cagttatttt
    48961 ataaccagta tcctttgatc agtaaaggct attctggtgt accgattatt ttaatgtatt
    49021 catcgatatt ttttatcaac atttttttta tgaccaactg gttccgacgg ttccatttgc
    49081 cacttacttc cagcacatat ttatttaggg gcaaacgtaa gatatatttt gccaaatagc
    49141 gatctttaag caggccattg atcacacggt cctcaagctg caagttgatc ccagtcagca
    49201 tcattggctg ttcttgtaaa acgtacgtat caattaaaag accttcaaaa gtttccacaa
    49261 gcgtcttcct ttcttttcaa agtaacctga aaacaggcac taattcttcc attttgatat
    49321 tcgttgaagt ggatgcgatc attctttctt ttgtccactg taatctagta taacaaaaaa
    49381 gagcagaaga gccaaaaaga aaaaagagca tcgaattcta taggaagtct cattggcacg
    49441 gcctaaatta ttacaaaaca ttgattaaaa aaagcccctg catcgcagga gcatccccta
    49501 tatcgtttta ataggtcaat caaaaagcag tatcaaacac attttgtatc aaatcaggaa
    49561 ataatagata agctaagccc agaatcaaaa gtaccgctag aagaaaccag ccgattgttc
    49621 ctatgaaata ctttaatttt tcgaacattg tatcacctcg ttgtcagtat atcacggatc
    49681 agtaaattgg gggtgttaag aatctcctaa aatagacaaa atttgttcta ttttcctaaa
    49741 gcaaatactt gtaagataca gtaagtgata ccagaagcaa tgaccggtcc ggcggcgatt
    49801 cctcttaaga agacaacacc taaaatcgtg ccgaaaacaa gtgcaacagt gatttgtgga
    49861 tcagaagcaa tcaatcctac acccttggaa gataagacag ccactagccc accgcaaaaa
    49921 acagctaccc agccaatagg ggatttaaag gcttctaata gatcacgtag accgatttct
    49981 cctgttgcga tagggattag aatagccgct gtgatgatcg tcaccccaat attgattcct
    50041 ttgccttgaa gccaagggaa aaaggaacgg gaggctggta tgagcttcat aatcaaaaca
    50101 aaagctgccg ctatcatcag actttgattt ttagctaaaa ttgctacaag taaaataatt
    50161 cctaaaaaaa gccaactttc catgaaatat attcctcact ttcttcgaaa caatcataac
    50221 atgtttttag aaagttacaa aagtaggaat tcatccttca agacctattc ataagctgtt
    50281 tttttcataa aataaacaaa atcataaaaa aacactttgc ttatttgaca gaagacagcc
    50341 gcgctttgta tagtggacaa gacttacatt tttttgaaga atcagaaaga tcaggtaaag
    50401 gtcttagtaa acgcaagcca ttcagaataa ccaatattgt gcttccttcg tgtcctacaa
    50461 ctccaagagg tagatttaaa aattgcagga agttagaagc aatcaacaag agaataacaa
    50521 gaatcgaaaa aataatattt tgtttgatga tccgccgcat ttttaaagat agccgatgac
    50581 tgtagacaaa tttatttaag tcgcttttca ttaagacgat gtctgcgata tccatagcgg
    50641 catctgtccc ttgccccata gcgacaccta aagaagcggc tgctaaagca ggcgcatcat
    50701 tgactccatc accaaccatc gcatttactt ggtaaagttc attctctttt ttgactagtt
    50761 gtgttttttc ttccggagta cagcttgcat agtaatgttc aatccctact tgttcagcaa
    50821 tggcctttgc tgtgccagaa tggtcgccgg ttatcatact tgtatggatg ttttgggatt
    50881 taaagtagga aattacttgt tgggtatttg cttttggaat atctagcaga cctaaaacag
    50941 caattaactg ctgatcttta gaaagataaa tcacggtttt gccttgattt tccaaacgtt
    51001 cgatcatttc cgcaatctct ttgctgatta tcatcgtctc aggatcatat gcgtgcttgc
    51061 caactttcca ttgcgcacct aaataggtag tttgtaatcc aaaaccagta atttcttcta
    51121 ttggtaagta ttggatttct tcaggtattg tgcaatcgaa atattgcaaa atcgcatgag
    51181 caagtggatg cgtcgttttt tgttccatag caacaagaag ttgttgtgcc aaatcctttt
    51241 catcaaagaa aaagctgtca gttacaactg gttttccttt agtgagcgtt cctgttttat
    51301 caaaggcaat cgcttttaat tcagaaagct tttctaaatg gataccgcct ttgattaaaa
    51361 tcccattttt tgcagaattt gaaagagcag ctagagaagc tggtgtagca gatgcgacaa
    51421 gcgcacaagg agaagccaca actaataata ccattcctcg ataaaagctt tcatcccaac
    51481 tccaacctag gaaaaagtgg gggagaagga tcattagagg aacagcaagc aaaatcaatt
    51541 ttacataatt attttcgatt cgttcgataa atgaagctgt ttgagttggc gtattctgcg
    51601 cttcttctac taattggata atcttagcaa agacagtttc ggaactcgtt tttgttactc
    51661 gaatcgtgat gggatttcct tgattcagcg ttcctgcata catctcatcg cctaaatatt
    51721 tttctacagg cacagattca ccgcttaatg cagactcatc gatcgttgcc tgtgtgcttt
    51781 ctaaaaagcc atctatcgga acagctgctc ctttagcgac aaataccaag ttatccacct
    51841 ttagttgaga aacatctact tcttgcattt gtccattttc ttttaataat tgggcttttt
    51901 gtggttgaag attcatcaaa gcagtaattt ctttttgact tttattagtt gtgtattcct
    51961 ctaatgcacc gcttaaacaa aagatgaatg tcaacattgc tccttcaaag taattaccga
    52021 tcaaacaggc accaatagca gctaaagcca tcaataaatc cacattcagt gtccgatcat
    52081 tccacaattc tttcaagcct tccgatgttt gtttatatcc accgatgata atggcactga
    52141 tataaaaaag tggggtgatg gtaagagaga atcggtcgaa gataaaaccg aagatcataa
    52201 ataaaaggca gccgattgtt gctgcaaaag cttttttctg attagtcatc gttattagac
    52261 ctccttgtca aaacaaggat agcataataa aaa
//
LOCUS       DACDKJ010000014        51636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDKJ010000014 DACDKJ010000000
VERSION     DACDKJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.014"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="IV328_000931"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="IV328_000931"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8937091.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="IV328_000932"
     CDS             1281..1727
                     /locus_tag="IV328_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8937092.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2000..3352
                     /locus_tag="IV328_000933"
     CDS             2000..3352
                     /locus_tag="IV328_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8937093.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3523..3810
                     /locus_tag="IV328_000934"
     CDS             3523..3810
                     /locus_tag="IV328_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937094.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4036..4479
                     /locus_tag="IV328_000935"
     CDS             4036..4479
                     /locus_tag="IV328_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8937095.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4485..5669)
                     /locus_tag="IV328_000936"
     CDS             complement(4485..5669)
                     /locus_tag="IV328_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8937096.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5937..6635
                     /locus_tag="IV328_000937"
     CDS             5937..6635
                     /locus_tag="IV328_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8937097.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6642..7319
                     /gene="nth"
                     /locus_tag="IV328_000938"
     CDS             6642..7319
                     /gene="nth"
                     /locus_tag="IV328_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8937098.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7445..7978)
                     /locus_tag="IV328_000939"
     CDS             complement(7445..7978)
                     /locus_tag="IV328_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937099.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(8275..10656)
                     /locus_tag="IV328_000940"
     CDS             complement(8275..10656)
                     /locus_tag="IV328_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8937100.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IV328_000941"
     CDS             complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IV328_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8937101.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            11357..11896
                     /locus_tag="IV328_000942"
     CDS             11357..11896
                     /locus_tag="IV328_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8937102.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            11986..12405
                     /gene="gpsB"
                     /locus_tag="IV328_000943"
     CDS             11986..12405
                     /gene="gpsB"
                     /locus_tag="IV328_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8937103.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            12427..12794
                     /gene="rnpB"
                     /locus_tag="IV328_000944"
     ncRNA           12427..12794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV328_000944"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13003..14169
                     /locus_tag="IV328_000945"
     CDS             13003..14169
                     /locus_tag="IV328_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8937104.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            14215..15711
                     /locus_tag="IV328_000946"
     CDS             14215..15711
                     /locus_tag="IV328_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8937105.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            15792..16475
                     /locus_tag="IV328_000947"
     CDS             15792..16475
                     /locus_tag="IV328_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8937106.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17120..18310
                     /locus_tag="IV328_000948"
     CDS             17120..18310
                     /locus_tag="IV328_000948"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8937107.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            18412..19365
                     /locus_tag="IV328_000949"
     CDS             18412..19365
                     /locus_tag="IV328_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8937108.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            19434..20783
                     /locus_tag="IV328_000950"
     CDS             19434..20783
                     /locus_tag="IV328_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8937109.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(20966..21289)
                     /locus_tag="IV328_000951"
     CDS             complement(20966..21289)
                     /locus_tag="IV328_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937110.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            21418..22581
                     /locus_tag="IV328_000952"
     CDS             21418..22581
                     /locus_tag="IV328_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937111.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            22651..24000
                     /locus_tag="IV328_000953"
     CDS             22651..24000
                     /locus_tag="IV328_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8937112.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24045..24476)
                     /locus_tag="IV328_000954"
     CDS             complement(24045..24476)
                     /locus_tag="IV328_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8937113.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            24864..27506
                     /gene="alaS"
                     /locus_tag="IV328_000955"
     CDS             24864..27506
                     /gene="alaS"
                     /locus_tag="IV328_000955"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8937114.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27706..28302
                     /locus_tag="IV328_000956"
     CDS             27706..28302
                     /locus_tag="IV328_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8937115.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            28424..29572
                     /locus_tag="IV328_000957"
     CDS             28424..29572
                     /locus_tag="IV328_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8937116.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(29610..30023)
                     /locus_tag="IV328_000958"
     CDS             complement(29610..30023)
                     /locus_tag="IV328_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8937117.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30102..30533
                     /locus_tag="IV328_000959"
     CDS             30102..30533
                     /locus_tag="IV328_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8937118.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(30632..30865)
                     /locus_tag="IV328_000960"
     CDS             complement(30632..30865)
                     /locus_tag="IV328_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8937119.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31048..32718
                     /locus_tag="IV328_000961"
     CDS             31048..32718
                     /locus_tag="IV328_000961"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8937120.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            32865..33251
                     /locus_tag="IV328_000962"
     CDS             32865..33251
                     /locus_tag="IV328_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937121.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            33238..33936
                     /locus_tag="IV328_000963"
     CDS             33238..33936
                     /locus_tag="IV328_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8937122.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            33941..34405
                     /locus_tag="IV328_000964"
     CDS             33941..34405
                     /locus_tag="IV328_000964"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8937123.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            34402..35313
                     /locus_tag="IV328_000965"
     CDS             34402..35313
                     /locus_tag="IV328_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8937124.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            35548..36087
                     /gene="pyrR"
                     /locus_tag="IV328_000966"
     CDS             35548..36087
                     /gene="pyrR"
                     /locus_tag="IV328_000966"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8937125.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36101..37384
                     /locus_tag="IV328_000967"
     CDS             36101..37384
                     /locus_tag="IV328_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8937126.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            37397..38323
                     /locus_tag="IV328_000968"
     CDS             37397..38323
                     /locus_tag="IV328_000968"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8937127.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            38342..39622
                     /locus_tag="IV328_000969"
     CDS             38342..39622
                     /locus_tag="IV328_000969"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8937128.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            39627..40703
                     /gene="carA"
                     /locus_tag="IV328_000970"
     CDS             39627..40703
                     /gene="carA"
                     /locus_tag="IV328_000970"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8937129.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            40708..43893
                     /gene="carB"
                     /locus_tag="IV328_000971"
     CDS             40708..43893
                     /gene="carB"
                     /locus_tag="IV328_000971"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8937130.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            43947..44723
                     /locus_tag="IV328_000972"
     CDS             43947..44723
                     /locus_tag="IV328_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8937131.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            44720..45652
                     /locus_tag="IV328_000973"
     CDS             44720..45652
                     /locus_tag="IV328_000973"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8937132.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            45649..46365
                     /gene="pyrF"
                     /locus_tag="IV328_000974"
     CDS             45649..46365
                     /gene="pyrF"
                     /locus_tag="IV328_000974"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8937133.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            46372..47061
                     /locus_tag="IV328_000975"
     CDS             46372..47061
                     /locus_tag="IV328_000975"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8937134.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            47034..47303
                     /locus_tag="IV328_000976"
     CDS             47034..47303
                     /locus_tag="IV328_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937135.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            47341..47982
                     /locus_tag="IV328_000977"
     CDS             47341..47982
                     /locus_tag="IV328_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8937136.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48013..48717
                     /locus_tag="IV328_000978"
     CDS             48013..48717
                     /locus_tag="IV328_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8937137.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            48714..49616
                     /locus_tag="IV328_000979"
     CDS             48714..49616
                     /locus_tag="IV328_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8937138.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(49774..51480)
                     /locus_tag="IV328_000980"
     CDS             complement(49774..51480)
                     /locus_tag="IV328_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8937139.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        16895 a         8983 c        11096 g        14662 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaatctta gatataaaag tgaatattat taaaataaga atcttgtaga
     1861 tattgggttc ttatgcttga aaaggaatga aactacaaac aaacctttgt gaattcacaa
     1921 gcattaaact gttgtatccg ttttatttct gttatagtag ttttgtcgaa ggacaaaatg
     1981 ttaatcggag ggaccataaa tgaaagtagt agtaattggc tgtactcatg caggtactgc
     2041 agctgtaaaa agtattttaa aaaatcatcc agaagcagaa gtgacagtat atgaaagaaa
     2101 tgacaatatt tctttcttat cttgcggtat cgcactttat gtcggcggag tagtaaagga
     2161 cccagcaggc ttgttctact caaatcctga agaattatct tcattaggag caacagttaa
     2221 aatggaacac gatgtaacag acgtcgatac agaaaataaa acagtaacgg caaaagatct
     2281 gaaaacagga gaaacagaaa cggtttctta tgataaatta gtgatgacaa ctggttcatg
     2341 gcctattatc ccaccgatta gtggaatcga tgcgaaaaat gtactcctat gcaaaaacta
     2401 caatcaagca aatgaaatta ttgcacaagc gaaagaagca aaacgtgtag taattgttgg
     2461 tggtggatat atcggtattg aattagtaga agcttttgtc gaatctggaa aacaagtgac
     2521 attgattgat ggattagatc gcatcttgaa taaatatcta gataaaccat tcacagatat
     2581 cttagaaaaa gaattgacag accgcggtgt tactcttgcg ctaggcgaaa atgttactga
     2641 atttatcaca gatgaagaag gaaaagtgaa acaagtagct acaccaacag atacatttga
     2701 tacagatatg gtcattttat gtgtgggctt ccgtccaaat acaaaactga tcgaaggaaa
     2761 agtagataca ctgccaaacg gggcaatcaa agtgaatgaa tacatgcaga caagtaatcc
     2821 agacattttt gcagctggag actctgcagt agtcaactac aatccaagtg gcacgcaaaa
     2881 ctatattcct ttggcaacaa atgccgttcg tcaaggtttg cttgtgggaa ataacttgac
     2941 agatcataaa atggcgtatc gtggtactca aggaacttca ggactttacc tatttggctg
     3001 gacgatcggt tcgacaggtg tgaccaaaga aagcgcaact ttgaacgatc tagaagtcca
     3061 agcgactgta tttgaagata attatcgtcc agagttcatg ccaactactg aaaaagtaat
     3121 gatggagcta gtctatgaaa aaggaacgaa tcgtatcgta ggtgctcagt tcatgtccaa
     3181 atacgacatt acacaatcag caaataccat gtctttagct gttcaaaata aaatgactgt
     3241 agaagatctc gcactttcag atttcttctt ccaaccacat tttgaccgcc catggaatta
     3301 cttgaaccta cttgcacaag cagcgctagg cgaaatcgaa actgcacaat aaagaaaaag
     3361 ggttgtgaca caattttgtc acgaccctct gtcttttttt gtgaaaagta caattagttg
     3421 tctttcttcg aattttttgt cacaataagc agcaaagata aagctgttac ataaaagaac
     3481 aagcagcttt atttgatttt gaaaagaaag gtgagaaaga agatgaataa agaatatttt
     3541 gtccaacaag atgcattttt agaagattat aaaagtgctt cgaagaaatt aacgatcaat
     3601 attgatgagt tggcagatca agtactctct cagttgaatg aaacgcaaga ggaatattac
     3661 acgatgccag caaatgacgc caaagataat gtttctcatc gttttccttt cagtagacgc
     3721 atctactcag atgaaaatga cggcacattg aatacagagt acacatatga aggtcagcca
     3781 tatgtgctag aaactgatga tgatctttaa agacagtttt tgaaaaggcc gctcatcctt
     3841 aagtgatgac aaaaaatcat cacaattacc gatgactgtc tctttactat cttttaatca
     3901 gaaaaagggg ttatgacaag ctttgtcaac cccttttttt cttggtttca gattgggttt
     3961 tcctacaaaa tatttttatc tttgttgaat aggaaacaaa aaaacaatat aatgaaagaa
     4021 aaagaaggtg tagaaatgga taaaagacaa ctgctaatag aagaatatct ggatttagct
     4081 aatgagatta ttcatcgtaa taaaccagaa atggaaaaag aatttgcagg atttactcta
     4141 aatgaactag aagtgattga acatatcggg aaaatccctg atcctaacgt gacaaaatta
     4201 gcgaatgcta gttacatgac tagaggagcc atcagtaagc taacaaaaaa actgatagct
     4261 agagggatca taaacactta tcaaagcgaa gagaataaaa aagaaatcta ttttcaattg
     4321 acgccaatcg gacaaaaaat aaggaataga cacgagcaac tccatcaaaa atgggtacag
     4381 cgagatgcta ctgtatttga aaatatgtca aaagaagaat ttgacacagt atttcgtttt
     4441 atcggtcgat acagagaatt tatacgaagt agcaaatgaa gctactattt tttattgtag
     4501 tgataatagg caaaaatcat aagcaaaaat ccgaataccg tcggaagaaa tggtgctgca
     4561 gcataaagct gtcctgcgat tagcgggccg atgacgcgcg atagagattg tatactttgg
     4621 gtaccgccca ttacaagacc ttgctggtta tctgagacag aattggatag ttggccattg
     4681 aatacaggag taaaaataga atcaccgaaa ccataacaga taatcccaaa taaaaacaaa
     4741 atcggcagta gaagaattcc agataatgtg atgaatagat aggcgaggat ttcactgaac
     4801 atccctattc ttgtgatctg ctgttctgaa aattttttga gcaatcgagg cataatgaac
     4861 aattgcgaga cagtatccaa gattccaatc aaagagaagc ttaaaccaac caacccagcc
     4921 ttccactgaa aggcgtcaat cgaaaattga gaaaagatag attgaagaga gccagctgcc
     4981 aaccaaacag caaaacctgc tgtcaataaa ggaataacgg atgaaatctt gaataattgt
     5041 tgtaattggt gaaaaggtct tacatgggag aaacttaaat ctactgaacg tttttttatt
     5101 ggaaggcttt ctagcataaa tgtgtagcca taaacggcat ttaagaaagt aaagagtgct
     5161 ccaataaata taggaacaga attccccaac tcagctaata gaccccctat aataggaccc
     5221 agcgttgttc ctataccgac taaagcaccc atccatccaa aaactttggt tctttcatta
     5281 ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga tttctcctgc cgtcaatcct
     5341 tcaatgatac gaccaaggaa caacatccat atcgaatttc ctaaaccaaa aagcaaatat
     5401 ccgatacttg atcctaataa actgataata aggatcggat gacgcccaaa atgatcactt
     5461 aagctaccga gaatgggggc agataagaag gcagctagag cataaacaga catgagcaaa
     5521 gtgatataga aagcttgctg atgtatggag tgagaataag gcagcgttaa aaacggaagt
     5581 acaggactga cgatagtaaa tcctaatcca gtgaataaga cagagacgta tccaaaaact
     5641 ttagaatgtt tatttgtgaa actgtacatg taaaaactcc tttatgcaat aatgttaact
     5701 tggaaacaaa aatagtgtat agtaattttg tttccttgtc aacattctaa ggaacagcga
     5761 aatctactta atttatttat tccgaaaagt gaagatacgc atcaaaaaca tgaacaaaca
     5821 aaaatcaagt agacgacatt gtaaatctga ccgatcagtg aagtaaatag aaaagaaacc
     5881 tgttcttgct tcccatgttt tttttacgta tactgtgatt gagcggagga gaaatcatgt
     5941 tgaatttaga aaattactta agcgctggac aaacagtcat atctaatctt ttacttaaaa
     6001 attatcatag gatcggtcta caatctgaag aatttttatt tgttctacag ttgcatatgg
     6061 cacagcttga aggagatact ttccctgatt tgcagatgat tgctatggat atgggaatca
     6121 aacaagatca gatttttcaa atactggatc gtctcgtaac aaatggattt atcaagatag
     6181 aaacgacgat tgtcaacggt aaaaaagcag atcgatataa tctttacccc atctatgatc
     6241 tattaggtga gtatttaaaa acacaagaaa aaaaatacga agaaaaaaca caagaaaaag
     6301 aaatccagtc agtgtatcaa ttatttgaac aagaatttgg acgcccgctt tcttcgatcg
     6361 agttccagcg gatcggacaa tggctagaag aagaccatta tcagccggaa attttgaagc
     6421 tagcactgcg ggaagccgta ttaaatcagg cttatagttt taattacgtt gaccgcattt
     6481 tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca agtggaagaa gaccaaaaac
     6541 gaagaaaaca gcagcttctt caaagagaag tagaacagtc agctcaacaa gaagaattgc
     6601 cgaaagtctc actgaaaaat tggttggagg agtagcggaa tatgttaagc aaacaaaaaa
     6661 caatggaagc tctagaaacg atgtatggaa tgtttcccga agcacatgga gaactcaagc
     6721 acaataatcc atttgagtta ttgattgcag tgatcttaag tgcccaggcg acagatgtat
     6781 cagtgaataa agcaacgcca gatttgtttg cctcttttcc gacacccgat gcgttagcag
     6841 aagcttcaat cgatgaaatc attcttaaaa taaaaacgat tggtttgtat cgaaataaag
     6901 caaaaaacat caaagcgtgt gcacagcagc tgattgaacg tttcgatggg caagtgccaa
     6961 cgtcaagaga agaattgatg tctcttcctg gtgtaggcag aaagacagca aatgtcgtat
     7021 tgggggatgc attcgggata ccagcaatcg cagtagatac ccacgtggaa cgtgtctcaa
     7081 aacgcctgag gatctgcaaa ctagatgcta cggtcatgga agtagaagaa acactgatgc
     7141 ggaaagtacc acaagaatta tgggtaaaga cacatcatac actgattttt tttggtagat
     7201 atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc tttgctatca atatgtcaag
     7261 atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa aaagaaacca cgcctgtaga
     7321 cgtggtttct ttaattgcaa ataaatgtat gaattatctt gttattatgg agaaaagctg
     7381 tttcgtttat cttttttgca tgaaaaagta aatgtacata ctcagtagct aggatggtaa
     7441 cttattacaa tttattgcga taaaaataaa agttgttatt tttattggga aaatacccga
     7501 tgtagaaaat aatgccttcc aatataccta taaaataagt ccagtattcc aaaggattat
     7561 ataaataagt gacgtatcta tctaaaggtg taataaaaat gataagtgat aataaaaaaa
     7621 taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc atcagaaaaa
     7681 tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga gagcttcttt
     7741 ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata aatgcaagga
     7801 taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct tttttattct
     7861 tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt atgataatta
     7921 agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc aaaaccatat
     7981 attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact aaataaaaga
     8041 ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta tatgtaatga
     8101 taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct
     8161 ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt
     8221 tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttactatct
     8281 gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat ccgctccgct
     8341 agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg agctttcttc
     8401 ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg ctgtcgtgct
     8461 ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc tttgagtcgt
     8521 tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa tagttgttga
     8581 tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt caccgcccac
     8641 acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg aaacatactg
     8701 cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga caggtgtcat
     8761 ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc ccgcaaatag
     8821 gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat ctgtgtaatt
     8881 ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg taccagttcc
     8941 ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg gagacatggc
     9001 tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga ttttattaac
     9061 atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg ctgccatttt
     9121 taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag agatcgcatt
     9181 cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag ccactttatc
     9241 tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt aaagtgcttg
     9301 gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag tcgaagtccc
     9361 ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca aatattcaaa
     9421 agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg aagtattcac
     9481 tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa tctgggcttt
     9541 tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt cgtctgggta
     9601 agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt ccagatccaa
     9661 attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt cttgtacttc
     9721 attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg attgagttag
     9781 ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg tttgagagat
     9841 tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg cctgatctgg
     9901 ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca atgctgtttg
     9961 cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg tctccatccc
    10021 gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct cttgtttcga
    10081 ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt tcagagtctg
    10141 atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca aagtacttcc
    10201 gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc gaatagggtc
    10261 aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat cttctaatgt
    10321 tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat cttcgaatag
    10381 ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt tatccgtcaa
    10441 ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa agaggataca
    10501 gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat gtgttccaga
    10561 ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag taggctgttt
    10621 ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct tcaatagata
    10681 atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa ataccagttt
    10741 tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt cctttttcct
    10801 tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag cgcttcaaag
    10861 acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct ttcatatgtt
    10921 cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg gtttcttttg
    10981 cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt gaggcttggg
    11041 taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact ttcacgattt
    11101 ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa tatttattac
    11161 tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg atctgatttc
    11221 ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg ccatttggat
    11281 agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa gcggatagaa
    11341 agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt atcgaagttt
    11401 cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa atgttttaaa
    11461 aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca gtgggaattt
    11521 gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat atccggaact
    11581 atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg aagggaacca
    11641 agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta gccatcaacc
    11701 ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc atacagtggg
    11761 ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt ttgaagatgc
    11821 acagaattac caagcagatc atccctacga aattcggctg atcagtatgg acgatcttca
    11881 aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct atacaatgaa
    11941 aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc gaatttggtt
    12001 tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg ctatgatccc
    12061 gttgaagttg atgaattctt agacaatatc atcaaagatt atgaagctta cagcaaagaa
    12121 ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca attatctaaa
    12181 actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag cgcagcagtg
    12241 accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt tggtaaaaag
    12301 cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa ctacacaaat
    12361 gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt gattaatgaa
    12421 tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt ccatgctcgc
    12481 acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag gtactctttg
    12541 gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct tgaaagtgcc
    12601 acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc ctcgagcgag
    12661 caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca aaaaggcagt
    12721 ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca aagtacagaa
    12781 catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag taatgcgtta
    12841 taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa tcgttcttat
    12901 tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg tggggtagga
    12961 ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa aaacaggaag
    13021 ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa agaattaaga
    13081 gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg aacaatggaa
    13141 acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat cgtggttggg
    13201 gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt gccttgggaa
    13261 gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa gtcgaaacta
    13321 tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc
    13381 tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt tcagttggaa
    13441 gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc aagtttattc
    13501 aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat ggcagcagca
    13561 cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt atgtggttca
    13621 ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg attcaatcgt
    13681 gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa tgttcgatca
    13741 gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc agatatcaat
    13801 ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg ctcttcgatt
    13861 acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg ggtcatcgtg
    13921 gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa attatataaa
    13981 gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt gactagcgac
    14041 ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt
    14101 gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa
    14161 gacgaataat cttactaatt gattcattca attggatgtt tggaggaata gggaatgaaa
    14221 gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc tttaggaatc
    14281 ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg aagtgaagta
    14341 gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc
    14401 attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc
    14461 aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat gagaaaatat
    14521 acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac acaacaattt
    14581 tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt gataccttac
    14641 tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga acccgggatg
    14701 actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct ttcgatccgc
    14761 cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg gagaatgacg
    14821 aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta tgattttagt
    14881 cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc gaatgatgca
    14941 cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac
    15001 gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta tacaccggtt
    15061 tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga gatcattatt
    15121 ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga
    15181 acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac aaaatcaagt
    15241 ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat tcgttatgaa
    15301 atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga aatatggaat
    15361 caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg catcctacaa
    15421 gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt agggtacatg
    15481 tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa tgttctagcg
    15541 tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca atatggtgca
    15601 tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa tccgcaatac
    15661 ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata aaatacaatt
    15721 tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta cttaagaaag
    15781 aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac caattagcgc
    15841 atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa
    15901 ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct tacctacatg
    15961 atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat
    16021 tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc attatcgaaa
    16081 atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa ggtaagatcg
    16141 ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt actgcttatt
    16201 tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa acattcaagg
    16261 acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa aaactgttct
    16321 tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga agagaaacct
    16381 tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat gaaaatttta
    16441 ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag aagtggtcag
    16501 ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg tttaccaaga
    16561 ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa atagcttctc
    16621 gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc gtttattcgg
    16681 aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca acaagtaaaa
    16741 cttttaagaa atattcagag agtaaaatta aaaattgctg ataaacgaag atggaaattt
    16801 tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac aattgatttt
    16861 aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag agtaaaatag
    16921 atcattatct aaaaggtaat tgttcatact atataagtca tttgaaagta aaaagatact
    16981 gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat caaaaatata
    17041 gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg caagacagat
    17101 gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag agaaagaatt
    17161 ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa gtgaaaatgg
    17221 tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca attatcttgg
    17281 atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa cttatggtgt
    17341 aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc aattagaaga
    17401 aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg gattcaactg
    17461 caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat cggatgaatt
    17521 aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca tccgaattaa
    17581 acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa gtaagaaata
    17641 taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca ttgcacgatt
    17701 gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg atgatgcgca
    17761 cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat taagcgacaa
    17821 aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg gttatgtagc
    17881 tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc tattttctac
    17941 ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga tgcaagaaca
    18001 tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag aattgaaaaa
    18061 agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt tgggagacga
    18121 aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg ctaaaccaat
    18181 cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga ctgcggaaca
    18241 tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa aagaaatgga
    18301 aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat gttcacgcat
    18361 atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat catgaaaaaa
    18421 gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg gctacgtcaa
    18481 gaactaggca gtgaaaatgt cattgcgaca gatatacgca tgcctgaagt cattgagaaa
    18541 aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag catagcagaa
    18601 caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt tgcagaagca
    18661 aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgcatt ggaagtcgct
    18721 agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg tccaaatact
    18781 ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta tggggtaacg
    18841 aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt agatactcgt
    18901 ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg tacgacagac
    18961 tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag tttcattgat
    19021 caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat tataaaactc
    19081 atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc ggcgatgtct
    19141 tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt tgaaatggat
    19201 tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc actagatgtt
    19261 tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt gatgacaaag
    19321 gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa gaaccacatc
    19381 caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa gggatggaag
    19441 aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa cgtacgatgg
    19501 gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg gtatttttta
    19561 aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt tcttggtttc
    19621 tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca gctgctattc
    19681 cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca gcagcttttt
    19741 tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc ttgtcgatca
    19801 ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt gttcccgtag
    19861 ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa gctggagggg
    19921 cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc gtcattttcg
    19981 gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca ggagaaaatc
    20041 tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca tacgacggtt
    20101 ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat ttgccaaaag
    20161 caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat gcggtgttct
    20221 taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt gacgttgcaa
    20281 aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg atttctgttt
    20341 atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt atggcaaaag
    20401 aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact aaagttcctg
    20461 tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc ggtggttttg
    20521 atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg gtctttgttg
    20581 gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa gttccaatgt
    20641 atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca agcacgctta
    20701 tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt atcccgattt
    20761 atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact gcacttctct
    20821 gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa aagtactcgt
    20881 taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta aaagacagaa
    20941 atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact attcggcgta
    21001 cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga agccaaatca
    21061 gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta tccatttcaa
    21121 gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa ataatcagga
    21181 gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc gtagcataaa
    21241 ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc tcgacacccc
    21301 ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc gttacttgct
    21361 tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag gagtcatatg
    21421 gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt agccttagcg
    21481 atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct agtaacaagg
    21541 gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt ggtgatctta
    21601 ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga gctgcattta
    21661 tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga acctagtacc
    21721 tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt tttattactc
    21781 ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa aacgatgatt
    21841 gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact ttattttatc
    21901 tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac attgggtgga
    21961 atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag aagacgaatg
    22021 gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt ggtcgcttat
    22081 ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt ctttaaaatt
    22141 gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga agtaggggta
    22201 tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa aattgtgcgt
    22261 atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat gatccgctac
    22321 gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct tcagcaaatc
    22381 atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc tctcggtgtc
    22441 ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag aaaaaaacgg
    22501 attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg ccaaacaaag
    22561 gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata atgccaaagt
    22621 cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt ccgcttcaaa
    22681 ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga ggtccaggaa
    22741 cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca aactggtaca
    22801 ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga aaaagaggaa
    22861 gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta tgaagcagct
    22921 acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt cggcggtaca
    22981 gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt agtaggcacg
    23041 cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac cgcatttgca
    23101 tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga agtagaccaa
    23161 attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac tattccagaa
    23221 aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga aatcaaacca
    23281 aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg taaagatgag
    23341 aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat cgtatttgcc
    23401 aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg cttgaaagta
    23461 gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg acaagtacag
    23521 aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat cgatatcgaa
    23581 ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt tatccatcgt
    23641 gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata ttcgccaaat
    23701 gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc aaaagaaatc
    23761 aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg agaaaaatca
    23821 aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa aaagatcaag
    23881 cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa agaacgcaaa
    23941 atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc acataaataa
    24001 aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc tagacttttt
    24061 ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt atgataccct
    24121 tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt ctgacaacct
    24181 tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc ttttcctcgg
    24241 tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt cttttcataa
    24301 cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg ttgcgggata
    24361 tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa aacttgatat
    24421 ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt tttcatcata
    24481 ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc gtaccaaaaa
    24541 tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca gtgatcaata
    24601 gaatgcgtat actaattaag tacgaaaagg atatgatttg ttttttaaga ctcacagaaa
    24661 attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt ttctattttg
    24721 gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt acacatcatt
    24781 gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt tttgtattta
    24841 atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt cgacaaatgt
    24901 ttcttgattt cttccaatct aaaggacata cgattgaacc tagtgcgtct ttgatccccg
    24961 tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa aaatattttg
    25021 atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct attcgaacaa
    25081 atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag atgctgggta
    25141 atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg gaatttttga
    25201 ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt tatccaaaag
    25261 atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat cacattattg
    25321 atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat acggaaattt
    25381 tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa aactatccag
    25441 gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa ttcaaccata
    25501 agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg atgggattgg
    25561 aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac ttgtttatgc
    25621 ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa aacccagtaa
    25681 cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt gctattggcg
    25741 acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg cttcgtcgtg
    25801 ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt ttagtccctg
    25861 ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa gaatttattg
    25921 aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa ggtttggaaa
    25981 tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta gcaggaaaag
    26041 acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa gaggtggcag
    26101 aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat gcacaacgcg
    26161 aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct gctttattga
    26221 cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca gatagtcaat
    26281 tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt gaagctcaaa
    26341 tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg gatcaaggat
    26401 ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa aaagcgccta
    26461 atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga caaacctatc
    26521 atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca gcaacccatc
    26581 tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct ggttctttag
    26641 tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact tcagatgaat
    26701 tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca gttgaaacaa
    26761 ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt ggtgaaaaat
    26821 acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg tgcggaggca
    26881 cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa tcaggaattg
    26941 gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa ctacttgaag
    27001 aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa ttgaaagaag
    27061 ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag gaaaatgaac
    27121 aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat ccagaaacga
    27181 tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat cagctgcgtc
    27241 aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta gcaaacgaac
    27301 aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa gctggactaa
    27361 aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt ggtggacgtc
    27421 cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca cttaaagctg
    27481 cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac aaaagttcaa
    27541 acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct catacttttt
    27601 tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag ttgacgaaag
    27661 tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa tagtagaaat
    27721 aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt acaagcgatg
    27781 gttccttggc tggggtttat tccattagga ttcattagtt taacaattat ccatatcaca
    27841 gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg cttgttttgg
    27901 ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga tacattagtg
    27961 ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt agcaggcttg
    28021 gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc ctttgcaggg
    28081 gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt attttataca
    28141 ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac cttagcaggg
    28201 gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac accattacta
    28261 gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc ttttaatagt
    28321 aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc gtcctctcca
    28381 tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta agaaacaaga
    28441 agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag cgggtatctt
    28501 aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc ttttttcagg
    28561 aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt catccatatt
    28621 gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc cttatggaca
    28681 cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt atgtaggatt
    28741 tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct ctttaacacc
    28801 catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag gcaaaatgta
    28861 tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa cagatagtta
    28921 taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat gggtaactgg
    28981 atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt atagtggatt
    29041 gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc cagcagagat
    29101 tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc atgatttatt
    29161 ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag tagatgaatc
    29221 aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact ttaaaaagtc
    29281 tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg aaaaatacta
    29341 taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc ttaatattca
    29401 tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg tccctgagaa
    29461 attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag aagaaaaaat
    29521 cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt aaatgagaaa
    29581 ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt taattgttta
    29641 tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg caaccattgc
    29701 aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta gggaaggaag
    29761 agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct gtctgaaaaa
    29821 cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt ttttaaaaag
    29881 acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag ctgttcttgt
    29941 ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc ttttgtagag
    30001 gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa ctaatgttat
    30061 ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa aatattcgat
    30121 ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc attctgtttt
    30181 ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat tttgttttag
    30241 gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta aaatcaaact
    30301 ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc ggacagattc
    30361 gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag aaagccccat
    30421 ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt gaaaaaaacg
    30481 gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc taattttaaa
    30541 aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac gtacggttca
    30601 tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta tcggtagtct
    30661 gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg atgatctcaa
    30721 attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc gcagtaaaat
    30781 gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt ttgttgtcaa
    30841 accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct gatcatagcc
    30901 ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta attttatgtt
    30961 ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat attttgttga
    31021 ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc acaagaaaca
    31081 gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga cgatttggaa
    31141 ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc aaaggataac
    31201 gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg cgaaggaaag
    31261 tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa atctgttatt
    31321 gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc tgcaggcgga
    31381 ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg tgacatgcat
    31441 gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat tcatcaagga
    31501 aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga tctcaatgat
    31561 cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt tccaagagaa
    31621 gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct tgcggcagac
    31681 cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt tgaccgtcag
    31741 cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct gaaagatgca
    31801 atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca cgggggaccg
    31861 tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc attgagatta
    31921 gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga gaaattttta
    31981 gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt cgccactgtc
    32041 cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga gaatcttgat
    32101 gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga acaatatcag
    32161 ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga actcacactg
    32221 ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt atgggcagat
    32281 ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga tagaaaagaa
    32341 gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac agaaacaatc
    32401 gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca aaaacaaata
    32461 gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa aacacagtat
    32521 tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat cacgatccgc
    32581 gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga tgttatgacg
    32641 atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa tgacggagaa
    32701 gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt attttgacta
    32761 cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc attctttttc
    32821 tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa acgacattta
    32881 taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc aacaatgaaa
    32941 aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg ccctttacga
    33001 tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa gcaaagcctg
    33061 cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta tttctagcaa
    33121 ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca gttattgtgt
    33181 tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag gaaaattatg
    33241 gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat gagagacgag
    33301 atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc tcaaagaaaa
    33361 caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt aaggaatgcg
    33421 atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga ttggacagtc
    33481 aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct gcctcgattt
    33541 gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt aaagatagta
    33601 gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt agcattgatt
    33661 actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc ggttgattta
    33721 acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa ttatcatttt
    33781 gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca agggactttg
    33841 gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg gatcgtacga
    33901 cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta tttattggat
    33961 attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca ctgataatct
    34021 agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc atattcgaaa
    34081 cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta tagtgacact
    34141 cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag gtaatcgctg
    34201 gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta tcgaccgtat
    34261 tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc cgatttttaa
    34321 cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata ttattttaga
    34381 tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca tgaatctggt
    34441 aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca aattcagctg
    34501 tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa ttacaaagta
    34561 aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga tgtggtccca
    34621 gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat aaacaaacct
    34681 caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt caatgcgctg
    34741 atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg tattgttcac
    34801 cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca ggcacacgaa
    34861 tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc tttagtccat
    34921 ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc gaaggaaaac
    34981 cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt tactgtattg
    35041 gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag aacacaccag
    35101 atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt atacggaccg
    35161 aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg ttttacgcac
    35221 ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt tgaaaaaact
    35281 ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag taattaacaa
    35341 cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct aagaagggtg
    35401 ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag tatagctatc
    35461 agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc aggagttttt
    35521 ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga tcaggtaacc
    35581 atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca tagtatccaa
    35641 gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag aattgccgaa
    35701 cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat cactttatac
    35761 cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga catcccagtt
    35821 tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg acgaacgatt
    35881 cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc attagcagtc
    35941 ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg gaagaacatt
    36001 ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg acaagatcgc
    36061 atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga aaaaaggatt
    36121 tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac attgggtagg
    36181 attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc ccatcttagt
    36241 aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg tgtacttatt
    36301 tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca ttgcagcgat
    36361 gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag tcacaactgg
    36421 attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat ggctggataa
    36481 aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg gacttgcggc
    36541 aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt atattgctgt
    36601 agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag gttttctcgg
    36661 attgattccg attttactag gtattattag cggatacatc gtagccttgc tgtttggaat
    36721 cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact ttgagattcc
    36781 ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg cacccatcgc
    36841 ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac taactaaacg
    36901 taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat tagcacaaat
    36961 cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca ttggtgttct
    37021 ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat ttgcagtcat
    37081 tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac cagtcatctc
    37141 aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga ttttaatcga
    37201 gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta tcttagtagt
    37261 aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa tggcattagc
    37321 aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa gcgaagaaaa
    37381 ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc atttattgac
    37441 cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc aggaattcaa
    37501 gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc tctttttcga
    37561 aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag ggctagaagt
    37621 gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat acgatacagt
    37681 actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg atgagaacta
    37741 ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg gaggagacgg
    37801 aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg aagaatttgg
    37861 tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc gcgtagctaa
    37921 atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag gtcctagaga
    37981 atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg aactactaga
    38041 tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg gaaaagaaag
    38101 tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg cgaagaagtt
    38161 gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag aattagctga
    38221 ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg gagtattcgt
    38281 cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg aggatgaaaa
    38341 aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga cgacaccagc
    38401 agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct cagaagccga
    38461 atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag tggacgtcca
    38521 tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg ggactcgctc
    38581 agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc ctgtacctga
    38641 tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag ttgtgaaggt
    38701 attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg atcaagaagc
    38761 actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc aaacagctgg
    38821 gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag tagcccatac
    38881 agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg cagaagaact
    38941 tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag atctgttgct
    39001 agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag aaagtgtccg
    39061 tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt caccccatca
    39121 tcttattttg atcgacgaag atattcccga agatttcggc ttttggaaaa tgaacccgcc
    39181 gctaagagga cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg gtacgatcga
    39241 ttgcatcgct acagaccatg ccccccatgg attagaagaa aaaagccaaa gtttcatgaa
    39301 atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata ctcattttgt
    39361 agaaactggt cgattcacgt tggaacaagt gatcaattgg ttggcagtta aaccggctga
    39421 gatttttggt ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg tagcagtttt
    39481 tgatattaca caaacttgca cgatcgataa agaagacttt ttatcaaaag gggagaatac
    39541 accttttatc ggctggaaag taaaaggcga aacacaaatg acttttgtca acggaaaact
    39601 tgtgtggcag aagggagaat aaaagtatgg aacgtttgct tatattagaa gatggaacag
    39661 ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc gtttttacaa
    39721 caagtatgac tggttatcaa gaaactatca ctgatcaaag ttttaacggt caaatcatta
    39781 catttaccta tcctatggtg ggtaattatg gggtaaatag agatgattat gaatcgattt
    39841 ctccaacatg caaaggagta gtagtaaaag aacatgcacg tgtagcttca aactggcgaa
    39901 accaaatgac gctagattca tttttgaaac aaaaaggaat tcctggaata tcaggtatcg
    39961 atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac gatgaaagct agtatagttg
    40021 atgctggaga ttccttcgaa catgcatttg atcagctgaa agcaaccgtt ctagcaacca
    40081 atcaagtgca gcaagtctca actaacaaac cttatccaag tccaggaaca ggtcggaaag
    40141 tcattgtcat tgacttcggc ttgaaacata gtattttacg agaattgagt aaaagaaact
    40201 gtaacctcac agtacttccc tataacacag atgccgagac gattttagcg ctatcaccag
    40261 acggagtcat gctgacgaat ggacctggtg atccaaaaga tgtgccagaa gcaatcgaaa
    40321 tgatcaaaaa aatccaagga aaagtaccga tttttggtat ttgtctcggt caccagttat
    40381 ttgctttagc caacggtgca gatacctata aaatgaaatt cggacatcga ggattaaatc
    40441 acccggtacg agaagtagcg accggaagaa ttgattttac ttctcaaaat catggctatg
    40501 cagtcgatga agccacgatt gatccagaaa aactcatcgt aactcatgta gaagtaaatg
    40561 acggaacagt agaaggagtc aaacacagag attatcccgc tttcagtgtg cagtttcatc
    40621 cagatgctgc ccctggtccg catgatgcac tacatttatt tgatgaattt atggaaatga
    40681 tggacgcagg aaaggagcag taaacagatg ccgaaacgta cagatatcaa gaaaattatg
    40741 gtgattggat ctggtccgat catcattggt caagccgcag aatttgacta cgcaggaaca
    40801 caagcctgcc ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa ttctaatcct
    40861 gcgacgatca tgacggataa agaagtggca gatcaagtat atatcgaacc gatcacactt
    40921 gaatttgtat caaggattct tcgaaaagag cgtccagatg ctctccttcc aactttaggc
    40981 ggacagacag gcctcaatat ggcaatggag ctggcagcat caggtatttt agaagaattg
    41041 aacatagaac ttctaggaac aaagctttct gcgatcgatc aagcagaaga tcgtgatcta
    41101 ttcaaacagt tgatgaaaga attgaatcag ccaatccccg agtctgaaat cgtcaataca
    41161 gtagaagaag ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt tcgtccagcc
    41221 tttactttag gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt acgtatcgta
    41281 tcagaaaatg gattaagtct ttcccccgct acccaatgtt tgattgaacg aagtattgca
    41341 ggttttaaag aaattgaata tgaagtgatg agagattcgg ctgataatgc aatcgttgtc
    41401 tgcaatatgg aaaattttga cccggtgggt atccatacag gtgattctat cgtatttgcc
    41461 cctagtcaaa cattgtctga ccatgaatat caaatgcttc gtgatgcttc tctttcgatt
    41521 attcgagcac ttaagatcga gggaggctgt aatgtccaac tcgctcttga tccacatagt
    41581 tttaattatt atgtgattga agtcaatccg cgtgtttctc gttcatctgc gttagcaagt
    41641 aaggcgactg gctatccgat cgctaagcta gctgctaaaa tagcgcttgg attaacatta
    41701 gacgaaatga aaaatcctgt tacaggaacg acttttgcag aatttgaacc tgcattagat
    41761 tatgttgtag ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg agaacggctt
    41821 ttaggcacgc agatgaaagc aactggtgaa gtgatggcta tcggtcggaa tatcgaagag
    41881 tctttattga aagctgtgcg ttctttagaa atcggtacac accatatcga acttcctgag
    41941 ttggcagaca tcacggacga tgacctaatc caaaaagtgg ttcgagcaca agatgatcgc
    42001 ctgttttatg tagctgaagc tattagaaga gggtacacca tagaagaatt agcggatttg
    42061 acaaaaatcg atttattctt tttagataaa ctgttgcata tcgtagaaat cgaacagcag
    42121 ttgaaacagc agccgttgga tgacaagata ttgaaaacag cgaaacaaaa cggttttaca
    42181 gacaaaaaag tggcggagct gtgggaaata aacgaagaag ccgttcgcca gatgagaaaa
    42241 gaaaatgatt tgattcctgt atataagatg gttgacactt gtgcagcgga attcgaatca
    42301 aaaacacctt atttttatag ttcctatgaa tatgaaaatg aaagttcacg atcagaaaag
    42361 ccctcaattc tagtactggg atctggtcct atccgtatcg gacaaggggt tgaatttgac
    42421 tatgcgactg tgcattctgt caaagcaatc cagcaggcag gatacgaagc aatcatcatg
    42481 aacagcaatc ctgaaacagt ttcgactgac ttttctattt cggataaatt atattttgag
    42541 ccattgacct tcgaagatgt gatgaatgtg atcgagctag aacagccgat aggagtaatc
    42601 gttcaatttg gtggtcagac agcaatcaac ttagccgaac ctttgactgc tgctggtgta
    42661 aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga tttattcgag
    42721 caagcgctga aagagttgga tattcctcaa cctttagggg atactgctac aagtaaagaa
    42781 gaagccgtgg ttatcgcttc gagaattggt tatccagttc ttgttcgtcc aagctatgta
    42841 ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag atttagaaga ttacatggaa
    42901 catgctgtga aagcatctcc agaacatcca gtactagtgg accgttactt gcttggtggt
    42961 gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac tcatcccagg gatcatggag
    43021 catattgagc gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc acctcaaaca
    43081 ttatctgaat cgatcaagaa aacgatcgaa gattacacga tccgcttagc attaggtttg
    43141 aattgtgtcg ggatgatgaa tatccagttt gtcatccatg aaaatgaggt ttatgtgatc
    43201 gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac aggaattcca
    43261 atggcacaaa ttgcgacgaa agctattttg ggagagaaac taaagacact tggttatgaa
    43321 aacggacttt atccagaaac aaaagaagtt catgttaaag cacctgtctt ttcttttacg
    43381 aaattgcaga aagttgatac ttacttaggg cctgaaatga aatcgactgg agaagtaatg
    43441 ggatctgaca gacaattaga caaagctttg tacaaggcat tcgaagcttc tggattacac
    43501 ttgcctgaat atggagccgt attatttacg attgcggacg aaacaaaaga agaagcacta
    43561 gaattagcta aacgttttac agaaattggc tatagtctga taggaacgaa acatacagct
    43621 gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc tgaggaagca
    43681 gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa cacaagtggt tgtcaatacg
    43741 attgataaag atcggggaaa tgcttctaaa gacggtttca tcatccgaag agaagcagtt
    43801 gaacatggta ttccattgtt tacttcttta gacacagcag atgcgatcgt tcgagtgatg
    43861 gaatcccgtg ctttttcaac taaccctatt taacggcgag cacatgttaa gacaaaaaga
    43921 cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat gatggaaatc gttcagcaaa
    43981 aacagctggc accaaagatt tttgaaatga cattgaaagg tgatctagta aaagaaatgc
    44041 agcaaccagg acagttcttg catattctcg tgccgcgtgc agacttgcta ttacgccgac
    44101 cgattagctt gaatcatatc gaccacgaaa caaatacttg ccgtatcatt tatcgggtgg
    44161 aaggtgaggg gactagagtc ttctctactc tttctgccgg agaccattta gatgtcatgg
    44221 gtcctttagg caatggtttt gatttgtcca atcttcatac aggagacgaa gtatttataa
    44281 tcggtggagg aatcggtgtc cctcctctat atgagttatc aaaacagtta gtcgaaaaag
    44341 gaattaagcc aacgcatttt ttaggttttg ccacacatga agtcgtttat tatgaaaaag
    44401 aatttaatcg tctaggagaa acaagaatcg ctactgacga tggaacatac ggtatgcacg
    44461 gaaatgtcgg taatttattg ttaggaacga agacggaacc tacagctgtt tttgcatgtg
    44521 gttcaaacgg gttattgaag acagtggaac agctgttttc ttcgcatcaa aatgtccagt
    44581 tatcattaga atcccgcatg gcatgtggga taggggcttg ctatgcttgc gtttgtcata
    44641 cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga ccgattttta
    44701 aaataggaga ggtaattata tgaatgagaa tcctttagcg gtcaaacttc ctggcctaga
    44761 attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag aatacggaaa
    44821 atactatgat ttgaatcagc ttggatcgat catggtcaag gcaacgactc caaatgcccg
    44881 ctttggcaat cccacgccaa gagtggcgga aacaccatct ggcatgttga atgcaattgg
    44941 tttgcaaaat cctggtctag acgttgttat gcataaactt cttccagaat tagaggagta
    45001 caatgagtta cccatcatcg caaatgttgc tggtgcttgt gaagaagact atgtagaagt
    45061 ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc gagctgaaca tttcttgccc
    45121 aaatgtcaaa catggcggaa ttgcttttgg aacagattct gatgttgctt ttcaattaac
    45181 acaagcagta aagaaagttt cttctgttcc tgtctatgtc aaattgtccc caaatgtaac
    45241 agacatcgtt ccaatcgctc aggcaatcga agctggaggg gcagatgggt ttacgatgat
    45301 caatactctt cttggtatgc ggattgacct taagacaaga aaaccgattt tagccaatca
    45361 aacaggtgga ctctccggac cagcaatcaa gcctgtcgct attcgtttga ttcaccaagt
    45421 agcaagtata tccagccttc caatcattgg gatgggcggc gtgcagacag tggatgatgt
    45481 tcttgagatg tttatggctg gagcaagtgc tgtagctata gggaccgcga atttcaccga
    45541 tccgtatatc tgtcctaaac taattaaaga gctccctgtg cgaatgagcg aactaggcat
    45601 cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat gaatcaacta
    45661 gaccaacgac caattattgc attagatttt tcaactaggc aagaggtaga agactttctt
    45721 cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt gttctaccaa
    45781 gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt tttagatctg
    45841 aaacttcacg atattccgaa tacggtcgaa aaagcaatgc ggggattggc aaagcttggt
    45901 gttgatctca cctgtgttca tgccgctggc ggtattcgta tgatggaggc ggcaatgcga
    45961 ggattagaag aggggactcc agaaggtgga aaacgtccgc tacttctagc tatcactcag
    46021 cttacttcta caagtgaaga agaaatgcat gccgatcaac taatcgaagt ttctttagaa
    46081 aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag gattagatgg tgtggtttct
    46141 tctgcttggg aagtagaagc gatcaaagaa atggcaggag acgaatttgt ttgcttaact
    46201 ccgggtataa gaccagaagg aacagtcgca ggagatcaga caagagtagt cacaccttct
    46261 caggcaaaaa agatcggctc aacttttatc gttataggtc gaccaatcac tcagtcaact
    46321 aatccttata aagcatatca aacgattcaa acagaatgga gctaacctaa aatgaatgtt
    46381 gaacaaagta tcgcaaaaga cttattagaa attgaagcag tatttttaaa tccaagtaat
    46441 ccttttacat gggcaagtgg aatcaaaagc ccaatttact gtgataaccg aatcacgatg
    46501 agctatccaa aagtccgaaa agagatcgca aaaggattag caagcaaaat caaagaagct
    46561 taccctgaag tacaagtgat tgctggaact gcaacagcag gaatccctca tgccgcatgg
    46621 gtagcggaaa tactggattt gccaatggtc tatattcgaa gtaaggcgaa agatcatggg
    46681 aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga agatggttgt gatcgaggat
    46741 ttgatttcta caggcggaag tgtcttggaa gcagctgaag cagcaaaaag agaaggtgcg
    46801 gatatccttg gtgttgcggc tatctttacg tatgagttgc caaaaggaaa agcaaatttc
    46861 gaaaaagccg aaatccctct tatgacgttg accaattatt ctgtattgat tgaagcagct
    46921 ttagaagatc gttatatcga tgagcaagaa ttaactctgc taaaagaatg gaaaaaagat
    46981 cctgaaaatt ggcagattga agcgcgcttg atgaagatag agaaatccga tttgtgtctc
    47041 cagaatcatc aattggaata gcaaaaatcc aaaaactgat tgcgagaagc atcttttcat
    47101 caggtatgaa aagaaacttc tatcgtgatc agtttttgat ttttttaaaa ttcatttttc
    47161 taagactggt tagcagaaag aagagaataa tctcttctct tttttctatt atatatagat
    47221 tttctctatt ttttggtaag ggagctgttt caaaaatggt aaagagcgga aatgttgcga
    47281 atatcagtta tttaatgata tgatattcgg aaaaagaaat ctcaaaggga ggtaactttt
    47341 atggggttga atgaatttat tttaggattg aacaatttag agacgatcac aagggcacct
    47401 ggatttttta aatatacaga gcatacagtt gcagcacatt cgtatcgtgt agcatctatt
    47461 gcgcaagttc taggagacat tgaagaagcg aatggacaac cgatcgattg gaaactgctc
    47521 tatgaaaaat cattgaatca tgactacaca gagcgtttta ttggtgatat caaaacgccg
    47581 gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa cagttgaaga aaagatgacg
    47641 gacgaattca tcaaccaaga gattccagct gagttccaag aagtttatcg ccgtcggttg
    47701 ttcgaaggaa aagatgaaac cgtagaagga gaactattag ccatttcaga taaggttgac
    47761 cttctttatg aatcttttga tgaaatcatc aaaaataacc cggaaaaagt ttataaggag
    47821 atgttcattg aggcagtgac tacgattaaa agctttgaac atcggccttc tgttcagtat
    47881 ttctttaaag agatatttcc ggaactattg aaccaggaat tttatggaaa agaggcgttt
    47941 ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat aaaaatgata tgagatgcag
    48001 ggggaagtat agttgaaaaa gaatatggat gtcgaatggg gctattctgg agacaaaggt
    48061 ccggaaaatt gggcgagttt atgtgattgg tttgcgagag gtgcggaatt tccattgcag
    48121 tcgcctattc atttgaccaa gggggaagaa accaaaatag aaggagacac gctttccttt
    48181 cattatcaaa agcaacggtt tactgacaag gaattcaaaa acacgattca tttagtacct
    48241 tttgaccaac tcagttatgt agagttcaag aaagaacgct attatttgac agatattcat
    48301 ttccacttgc cttctgaaca tatcatcaac ggaaaacagg aagatattga atttcatttt
    48361 gttcatatga acagcaaaaa agagaatcta gttgtgggtg tcatgtacca gctaatggta
    48421 caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt ggaacttaga aaaacatgag
    48481 cattgggtaa atccggatgt gttctttccg gagcaaaaaa gccatttcca ttatattggc
    48541 tcattgacaa ctccgcctac cagcgggcca atccaatggt ttgtaatgga tcatgtagga
    48601 aaactgaacc aagagtttct tctccccttc gatggtcagt atgctcgacc gaacaaccga
    48661 ccaatccagc ccttgaatgg aagagaaatt tattatttcg aggagtttga gcaatgaata
    48721 ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg tagtttcaga gaagcggcaa
    48781 agaaaatgtt tgtctcccag ccaagtcttt cagctggtat caaagaatta gaaaccgagt
    48841 tagggatcac gctgtttact cgaacgaatc gaggggcata tttaacagaa gaagggcaag
    48901 acttcttaaa tcgtgccgag aaaatattgg tccagcttga tacattagaa actcactatc
    48961 taacgagtga aaagagagac aatttttcga tcgctgccca gcattatgat ttcttaggtc
    49021 ctttgaccgc acagctactc gaggaattca aagacgagat caaacaattt cggatcatgg
    49081 agacgacaac agcgagagtg atcgaagaag tcaaagagca gcacagtgag atcggcatta
    49141 tttatatgaa tgagcataat caagctggta tcagaagata tttacaacaa ggagagctaa
    49201 gtggccgcag cattggaaaa ttcaaaacgc atattttttt aaggcaagga catccattag
    49261 ctgaacacgc agagatcacc aaagaagaat tgttatctta tcctcaagtt cgttttacac
    49321 aggatggaaa taatttcccg tatttttatg aagaccttat cgagatccct gatcaagaaa
    49381 cagtgattta tacgagtgat cgaggtactt tgatgaatat cgttcttgaa actgatgctt
    49441 acgcttcagg atctggtatt gtgattggag ggattcgcga gcacttgcgg ttaatccctt
    49501 tggcagaagg cgaactaaat gaattttata tcatttattc ggcgaaacgt ccgctcagtg
    49561 aaatcgctca aagatttatc aaagagctta ctcggttact ggaccagcag agctaagaaa
    49621 agcagatgag aacgctgttt attgaacacc gttgatcaaa aaagaggggt tgtgacagaa
    49681 ttttgtcaca actcctcttt ttgtagcaaa caatttataa ctaattgctg ttgtgggaaa
    49741 gtacgcaaat atgattctag aaacactcat tgattacctg ttttgtttca tagctgtaat
    49801 tttttcttct tctggtgtca cgataatcgt cttttggttt tcataaataa cataaccagg
    49861 cttggcgcca tttggttttc ggatatgctt tacctgtaca agatcaaccg gcacttgggc
    49921 agagtatcgg tattttgaaa aataggcagc taactcggca gcttctgtaa ttgtttcatc
    49981 tgagggcttg tcacttttaa tgatgacgtg cgaaccgggt atattttttg catgaagcca
    50041 atagtcggtt tttttagctg tcttcattgt cagctggtca ttttgaagat tgtttttacc
    50101 aacaagaatg agcgttccgt cagaagaaaa gtattgatca ggctgagatg gtttcttacg
    50161 tttttgtttt tttaagttct tctttttcag gtatccttct gaagtcaatt cttctttaat
    50221 tgcttcgata tccattgggc cggcaatttc cagttgagat aagacagatt caagatattg
    50281 gatttcatct ttggcttctt ggatttgttc gccaatcagc ttaacagcgt tcttcagctt
    50341 ttggtatcga tggaaatatt tctgtgcatt ttgatttggt gtcaatgccg gatctagtgc
    50401 tattttgatt ggccgatctt cttcataata attaggtaaa acaacttctt ttgcgccgcg
    50461 tggtacttgt gtcagaaaag tagtaagtag ttcgccattt ctacggtaat tctcagcgtt
    50521 ttcggattct ttgagtgttt gttcacgttt tttcaattta tttttatttc gttttagttc
    50581 attttcgatt tttcgaatca gttctccgcc ttgttgtttt gcccgatctt tctctgcttt
    50641 ttcgtgataa tagctatcta ataattgact gagtgttgga taagctgtta cagcagatgc
    50701 ctgttcggac aatacctgat aagcaatcgg tgtgaaaaaa tctttatttt ccgtttcgta
    50761 aaaagtagga ataggctgat ggctgatggc tgagaaaaat tcgtcccata caaccatctt
    50821 ttcattagga cgttccagta atcttttggt cagctcttga gcgatatcga aaccgattcc
    50881 ctgaaactga cgttggattg cttttggatc tagttcagtt tgggaaagcc gctgaaagat
    50941 tttttctttc tcggagctaa atgggttcaa gacctcttgt tttggcggag caacatattc
    51001 tacaccagga agcaaagaac gataagtgtt ttgagagctt ccaatgtgtt tgatcgcatc
    51061 cagtattttt ccagtttctc gattaactaa gacgatcgta ctatgacgtc ccattaattc
    51121 aacgatcaat atgatatttt gtagatcacc caactcgttt cgtttagcaa agtggaaatg
    51181 gatcactcga tcattttcga tttgttcgat gtcttctaaa atagcgccat ccaaatattt
    51241 tcgaagcatc atcacgaagt taggcggatt atcaggattc tgataatcga tttgggtaat
    51301 ctgtacacga gcataacttg ggtgcgcaga taataataat cgatggtttt tgccgcgtga
    51361 acgaatcacc agcacgattt cattttcata aggctgatgg attttcgaga tccggccaga
    51421 tagtaacgtt tcacgcaatt cgttgaccat cgcgtgggta aatacaccat caaaggacat
    51481 ggttcattcc tcatttcttt tcataagtat attcattata acgaaaaaaa gaagaaaaac
    51541 caatttggcc gaatagtaaa gcaaaagtct agaaaaatct tggactaatt acataaaaaa
    51601 cagtgtttct atcgttgaaa tccaaatttt taggta
//
LOCUS       DACDKJ010000015        51224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDKJ010000015 DACDKJ010000000
VERSION     DACDKJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.015"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            290..1135
                     /locus_tag="IV328_000981"
     CDS             290..1135
                     /locus_tag="IV328_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8937140.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IV328_000982"
     CDS             1416..2636
                     /locus_tag="IV328_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8937141.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IV328_000983"
     CDS             2633..5353
                     /locus_tag="IV328_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8937142.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IV328_000984"
     CDS             5337..6281
                     /locus_tag="IV328_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8937143.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IV328_000985"
     CDS             complement(6350..7360)
                     /locus_tag="IV328_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8937144.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IV328_000986"
     CDS             complement(7540..7884)
                     /locus_tag="IV328_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8937145.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IV328_000987"
     CDS             complement(7877..8311)
                     /locus_tag="IV328_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8937146.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IV328_000988"
     CDS             8449..9186
                     /locus_tag="IV328_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937147.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IV328_000989"
     CDS             9179..10390
                     /locus_tag="IV328_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937148.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IV328_000990"
     CDS             10597..11376
                     /locus_tag="IV328_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8937149.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IV328_000991"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IV328_000991"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8937150.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IV328_000992"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IV328_000992"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8937151.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IV328_000993"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IV328_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8937152.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IV328_000994"
     CDS             14978..16027
                     /locus_tag="IV328_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8937153.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IV328_000995"
     CDS             16024..16302
                     /locus_tag="IV328_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8937154.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IV328_000996"
     CDS             16277..16747
                     /locus_tag="IV328_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8937155.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IV328_000997"
     CDS             16764..17066
                     /locus_tag="IV328_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937156.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IV328_000998"
     CDS             17083..17454
                     /locus_tag="IV328_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8937157.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IV328_000999"
     CDS             17426..17785
                     /locus_tag="IV328_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937158.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IV328_001000"
     CDS             17957..18964
                     /locus_tag="IV328_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8937159.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IV328_001001"
     CDS             18989..20173
                     /locus_tag="IV328_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8937160.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IV328_001002"
     CDS             complement(20610..22193)
                     /locus_tag="IV328_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8937161.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IV328_001003"
     CDS             complement(22771..22914)
                     /locus_tag="IV328_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8937162.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="IV328_001004"
     CDS             complement(23136..23615)
                     /locus_tag="IV328_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8937163.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="IV328_001005"
     CDS             complement(23688..23972)
                     /locus_tag="IV328_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937164.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="IV328_001006"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV328_001006"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="IV328_001007"
     CDS             complement(24316..25602)
                     /locus_tag="IV328_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8937165.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="IV328_001008"
     CDS             25740..26411
                     /locus_tag="IV328_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8937166.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="IV328_001009"
     CDS             26377..26865
                     /locus_tag="IV328_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8937167.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="IV328_001010"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="IV328_001010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8937168.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="IV328_001011"
     CDS             27826..28590
                     /locus_tag="IV328_001011"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8937169.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="IV328_001012"
     CDS             28823..31036
                     /locus_tag="IV328_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8937170.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /locus_tag="IV328_001013"
     CDS             31047..31493
                     /locus_tag="IV328_001013"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8937171.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="IV328_001014"
     CDS             31664..32923
                     /locus_tag="IV328_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8937172.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="IV328_001015"
     CDS             complement(33012..33959)
                     /locus_tag="IV328_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8937173.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /locus_tag="IV328_001016"
     CDS             34353..35645
                     /locus_tag="IV328_001016"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8937174.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="IV328_001017"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="IV328_001017"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8937175.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="IV328_001018"
     CDS             37538..38413
                     /locus_tag="IV328_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8937176.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="IV328_001019"
     CDS             38679..40562
                     /locus_tag="IV328_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8937177.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="IV328_001020"
     CDS             complement(40637..40921)
                     /locus_tag="IV328_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8937178.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="IV328_001021"
     CDS             complement(40911..41645)
                     /locus_tag="IV328_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8937179.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="IV328_001022"
     CDS             41774..42397
                     /locus_tag="IV328_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8937180.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="IV328_001023"
     CDS             42589..43710
                     /locus_tag="IV328_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8937181.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="IV328_001024"
     CDS             43717..46845
                     /locus_tag="IV328_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8937182.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="IV328_001025"
     CDS             46864..50058
                     /locus_tag="IV328_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8937183.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="IV328_001026"
     CDS             complement(50204..50911)
                     /locus_tag="IV328_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8937184.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16768 a         8945 c        10632 g        14879 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cgggacagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acagtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatat cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgaaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttctgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    51121 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt
    51181 taatacagtc tcaatataac tattcaaaca taaaataacg ttat
//
LOCUS       DACDKJ010000016        47953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDKJ010000016 DACDKJ010000000
VERSION     DACDKJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.016"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IV328_001027"
     CDS             complement(38..1189)
                     /locus_tag="IV328_001027"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8937185.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IV328_001028"
     CDS             complement(1202..1639)
                     /locus_tag="IV328_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8937186.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IV328_001029"
     CDS             complement(1654..3420)
                     /locus_tag="IV328_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8937187.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IV328_001030"
     CDS             3684..4361
                     /locus_tag="IV328_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937188.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IV328_001031"
     CDS             4364..5491
                     /locus_tag="IV328_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937189.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IV328_001032"
     CDS             complement(5691..6131)
                     /locus_tag="IV328_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8937190.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IV328_001033"
     CDS             complement(6142..8175)
                     /locus_tag="IV328_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8937191.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IV328_001034"
     CDS             complement(8177..9880)
                     /locus_tag="IV328_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8937192.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IV328_001035"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IV328_001035"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8937193.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IV328_001036"
     CDS             complement(10963..11988)
                     /locus_tag="IV328_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8937194.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IV328_001037"
     CDS             complement(12197..13066)
                     /locus_tag="IV328_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8937195.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IV328_001038"
     CDS             complement(13525..15417)
                     /locus_tag="IV328_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937196.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IV328_001039"
     CDS             complement(15414..17141)
                     /locus_tag="IV328_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937197.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IV328_001040"
     CDS             complement(17157..17807)
                     /locus_tag="IV328_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8937198.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IV328_001041"
     CDS             18011..18307
                     /locus_tag="IV328_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8937199.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="IV328_001042"
     CDS             18479..19099
                     /locus_tag="IV328_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8937200.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="IV328_001043"
     CDS             complement(19191..20357)
                     /locus_tag="IV328_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8937201.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="IV328_001044"
     CDS             complement(20369..20722)
                     /locus_tag="IV328_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8937202.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="IV328_001045"
     CDS             20850..21872
                     /locus_tag="IV328_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8937203.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22311..22745)
                     /locus_tag="IV328_001046"
     CDS             complement(22311..22745)
                     /locus_tag="IV328_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937204.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="IV328_001047"
     CDS             complement(22855..24714)
                     /locus_tag="IV328_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8937205.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="IV328_001048"
     CDS             complement(24832..25992)
                     /locus_tag="IV328_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8937206.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IV328_001049"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IV328_001049"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8937207.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="IV328_001050"
     CDS             complement(27739..28437)
                     /locus_tag="IV328_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8937208.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="IV328_001051"
     CDS             complement(28394..30055)
                     /locus_tag="IV328_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8937209.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="IV328_001052"
     CDS             30273..31673
                     /locus_tag="IV328_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8937210.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="IV328_001053"
     CDS             complement(31925..33004)
                     /locus_tag="IV328_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8937211.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="IV328_001054"
     CDS             complement(33090..34049)
                     /locus_tag="IV328_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8937212.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35584)
                     /locus_tag="IV328_001055"
     CDS             complement(34061..35584)
                     /locus_tag="IV328_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8937213.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="IV328_001056"
     CDS             complement(35594..36157)
                     /locus_tag="IV328_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8937214.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="IV328_001057"
     CDS             complement(36163..37026)
                     /locus_tag="IV328_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8937215.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="IV328_001058"
     CDS             complement(37040..37936)
                     /locus_tag="IV328_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8937216.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39382)
                     /locus_tag="IV328_001059"
     CDS             complement(38111..39382)
                     /locus_tag="IV328_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8937217.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="IV328_001060"
     CDS             complement(39372..40889)
                     /locus_tag="IV328_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8937218.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="IV328_001061"
     CDS             41087..41977
                     /locus_tag="IV328_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8937219.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IV328_001062"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IV328_001062"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8937220.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="IV328_001063"
     CDS             complement(42886..44358)
                     /locus_tag="IV328_001063"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8937221.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="IV328_001064"
     CDS             complement(44995..45357)
                     /locus_tag="IV328_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8937222.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="IV328_001065"
     CDS             45580..46332
                     /locus_tag="IV328_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8937223.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="IV328_001066"
     CDS             complement(46472..47395)
                     /locus_tag="IV328_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8937224.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="IV328_001067"
     CDS             complement(47410..47682)
                     /locus_tag="IV328_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937225.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13980 a         9954 c         8268 g        15751 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga aca
//
LOCUS       DACDKJ010000017        41016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDKJ010000017 DACDKJ010000000
VERSION     DACDKJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.017"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            269..537
                     /locus_tag="IV328_001068"
                     /pseudo
     CDS             269..537
                     /locus_tag="IV328_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            1209..4610
                     /locus_tag="IV328_001069"
     CDS             1209..4610
                     /locus_tag="IV328_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8937226.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(5034..5204)
                     /locus_tag="IV328_001070"
     CDS             complement(5034..5204)
                     /locus_tag="IV328_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8937227.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(5398..5577)
                     /locus_tag="IV328_001071"
     CDS             complement(5398..5577)
                     /locus_tag="IV328_001071"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937228.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            5964..7076
                     /locus_tag="IV328_001072"
     CDS             5964..7076
                     /locus_tag="IV328_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8937229.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(7154..7447)
                     /locus_tag="IV328_001073"
     CDS             complement(7154..7447)
                     /locus_tag="IV328_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8937230.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            7615..7929
                     /locus_tag="IV328_001074"
     CDS             7615..7929
                     /locus_tag="IV328_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8937231.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7950..9929
                     /locus_tag="IV328_001075"
     CDS             7950..9929
                     /locus_tag="IV328_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8937232.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            9942..10268
                     /locus_tag="IV328_001076"
     CDS             9942..10268
                     /locus_tag="IV328_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8937233.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            10284..10802
                     /locus_tag="IV328_001077"
     CDS             10284..10802
                     /locus_tag="IV328_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937234.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            10802..12154
                     /gene="celB"
                     /locus_tag="IV328_001078"
     CDS             10802..12154
                     /gene="celB"
                     /locus_tag="IV328_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8937235.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(12238..13743)
                     /locus_tag="IV328_001079"
     CDS             complement(12238..13743)
                     /locus_tag="IV328_001079"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8937236.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(13835..14425)
                     /locus_tag="IV328_001080"
     CDS             complement(13835..14425)
                     /locus_tag="IV328_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8937237.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            14604..16250
                     /locus_tag="IV328_001081"
     CDS             14604..16250
                     /locus_tag="IV328_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8937238.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            16299..17018
                     /locus_tag="IV328_001082"
     CDS             16299..17018
                     /locus_tag="IV328_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8937239.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            17497..18444
                     /locus_tag="IV328_001083"
     CDS             17497..18444
                     /locus_tag="IV328_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8937240.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            18456..19376
                     /locus_tag="IV328_001084"
     CDS             18456..19376
                     /locus_tag="IV328_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8937241.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            19417..20874
                     /locus_tag="IV328_001085"
     CDS             19417..20874
                     /locus_tag="IV328_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8937242.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            21219..21833
                     /locus_tag="IV328_001086"
     CDS             21219..21833
                     /locus_tag="IV328_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8937243.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            21826..23550
                     /locus_tag="IV328_001087"
     CDS             21826..23550
                     /locus_tag="IV328_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8937244.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            23547..24998
                     /locus_tag="IV328_001088"
     CDS             23547..24998
                     /locus_tag="IV328_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8937245.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(25471..27624)
                     /locus_tag="IV328_001089"
     CDS             complement(25471..27624)
                     /locus_tag="IV328_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8937246.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(27626..28672)
                     /locus_tag="IV328_001090"
     CDS             complement(27626..28672)
                     /locus_tag="IV328_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8937247.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            28822..30111
                     /locus_tag="IV328_001091"
     CDS             28822..30111
                     /locus_tag="IV328_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8937248.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            30124..32826
                     /locus_tag="IV328_001092"
     CDS             30124..32826
                     /locus_tag="IV328_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8937249.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            32811..33668
                     /locus_tag="IV328_001093"
     CDS             32811..33668
                     /locus_tag="IV328_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8937250.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(33739..34596)
                     /locus_tag="IV328_001094"
     CDS             complement(33739..34596)
                     /locus_tag="IV328_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8937251.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            34807..36978
                     /locus_tag="IV328_001095"
     CDS             34807..36978
                     /locus_tag="IV328_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8937252.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            37046..37387
                     /locus_tag="IV328_001096"
     CDS             37046..37387
                     /locus_tag="IV328_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8937253.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(37663..39417)
                     /locus_tag="IV328_001097"
     CDS             complement(37663..39417)
                     /locus_tag="IV328_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937254.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(39428..40264)
                     /locus_tag="IV328_001098"
     CDS             complement(39428..40264)
                     /locus_tag="IV328_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8937255.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(40266..40949)
                     /locus_tag="IV328_001099"
     CDS             complement(40266..40949)
                     /locus_tag="IV328_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8937256.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        13306 a         7157 c         8494 g        12059 t
ORIGIN      
        1 gttttataaa agtgttattt tttggaaaaa ttcacaaata tgtaacgaat gtttttctgg
       61 aatttgctgt gttaaaaagc agtatgacta atataaatac tattgtcatt tatgcaattt
      121 tttatggata gtaattttag aggggaatca atgaaggaag aatagaaggg aaaaatagtt
      181 gcaaatacgc cagttagcaa aaatagcaat acatcttcaa gctttaaaat tcgagagaat
      241 catcttggta gtggaatcaa ctacaacaaa aggattaaac caattaatag atagaattac
      301 caatggaaaa atagaaaagg tagttattct atacaaaagt tgtttaattc gcttcggtta
      361 tgaacttagt gaaacccttt gcaatatatg gtacagaata tagaaattat tgacattaca
      421 gaaaatgatg aagatttaat acagattgtc actatttgta gatacctttt acaaggaaac
      481 cgagccaata aagctaagaa gatgattaag gagttgatgg aagacgatac aagttaagaa
      541 agtgaaacta cgcccaaata aagagcaaaa agagtaattg gacgtggaaa atgcaagaaa
      601 tgaaccatcg attcggtgga cagtttatta gtaatgacgg tttgagaaca tccatgacga
      661 gaatgaagga acatcctaaa tgcgtttagt tgaaccaagt gccaaataat atggccaaat
      721 aagcgatcag ttatcgatga atcccaagta ttctaaacta tatattcatt ttgatgctaa
      781 gcattgatat atttttttgg gattgatgaa ggttaaccaa tacagggatt cactgatgaa
      841 attctagagt ctgaccttgg tatcaacgat ttagtgattg tttctaatgg aaaagtataa
      901 aagtatataa gtacattaac aaaataacta ttagggttac gctccagctt gtgcaaggca
      961 ctggcaggag gaatcgtgaa atcataatta tggttaagcc acacggacaa ctaaaagtca
     1021 tacaaagcta tcgaatcaat caacagaaga caatcggcaa tcattagctt tgtttacagc
     1081 ttacattgca acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag ccattttgag
     1141 ttggggaatc tttttagaaa attatcgggt accttgttgt ttagtcaaaa ttataagggg
     1201 gagtaatagt gaggaacaaa tttcataaac tgttaagtat gtttctagtt atgcttattg
     1261 tgatcaatca atttgggaac ataacggttc tagctgagtc tttagcagaa cagccagagc
     1321 gttcgctgac gatcgaagtt gatcaagagg aggctgcttt gggtgagcag ttccagttgc
     1381 gagtcagagg aacaccagac ctgctaaatg cattggtcat tgataagagt gaggatttga
     1441 ttgaagggga agtgaagcaa ctcaatgatt acgaacgtct gattacttat tcttcccagc
     1501 attcaggtaa atacgaattg accgcaagaa gcggtgatat tcaatcaaat acggtctcaa
     1561 tagaaatcag tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca gaagaaagca
     1621 caagtgaatc ttttaatata ccagaagaaa gcttaagtga atcttttaat gaagccagat
     1681 catctgagcc atctataacg caggaatctc agattgaaag acaagaggag ctcttgaatt
     1741 atgacatcgg catctctgct gagcgcccca ctttgccgag caggcataca caaacgttaa
     1801 agggatacat tcaaggaggc gctggcgata ttcttgaaga atatggggag atggtcgtac
     1861 gagttcctag agcatcgatt ggcttaattg agttcattag agtggaggtc gctgatgatt
     1921 cacctttaaa gccactggca ttttaccaag aaggcacaga cgttgttttt ccatttacct
     1981 ttgattatga aggattaaat gaagccgctc agtcgtatac attcagtatc acgtttgaag
     2041 ttgctttctt tcaatcaggc ggtactgagc caaataatga ctttcaagtg agtcttactt
     2101 atgctgagcg agcagcaaac acggaatttt caattacgcc tattccttca tcaagaccca
     2161 ttttttcgaa attatactat ggctctagag aaatcgacag ccaaggaaat cttgggaatc
     2221 tgctgattga cgggcaagac gtgaccaaca gtaataaatt tattttgcag gtaaattatg
     2281 aaggatcacg aaaagacggt gtagtggtta ccgatgtact accgcaagga acaactttgg
     2341 caccgaatcc gactacatgg agttccactg gaaatagaga acctatttca ggtggggttc
     2401 ggatcgcaga agtaacgaat ccaggtgaag caaacgttag aactcattat gtttccgagg
     2461 aatttgtgga caaagtatct tttaataagg aaacgaatac acttgttgtg gactttggta
     2521 atctagcaga gggtcaatcg tattttattg aatatgctct tgaagtgacc gatgtcgact
     2581 tgttcaatga aggaagacaa gaggatgggt tttctcggaa tcgagcgagt atgacctaca
     2641 ataatcaggt aatgagccga gactaccgct tgagaatccc tgaaagtaat cttccggcaa
     2701 tctctttaag aaaaagtgtg gacaaaaatg ttatcaatct caatgaaaat gaactaacgt
     2761 atactctgca tgtctcgatg ttttctggaa caagaaatac gcttgttatt gaagatgttt
     2821 tggaagaagg catggaattt gtgtcatttc tatcagatat tccctctttc attgatgccg
     2881 aagtcaataa tggaatagtt cgtttcagtg cgaataatac gattggtggt ccgacaagtt
     2941 ttaccctctc ttttaaggtc aatgtagagg cgtatgcggt cggcgatcaa attttaaact
     3001 ttgcaacgct tgtgaatagt gacaatcgat tgaatacgag tactgtaaga acaaggaaaa
     3061 tcgatgggcg tattaaagta attaaaactg atgagaatgg ccaaaggctg gcaggagcaa
     3121 aatttgaaat tttatcaagc gattcacaag tgatacaaga aggtataacg gcagaaaacg
     3181 gagaattttt aagtgaaccg cttcctgtag gtagctatca agtccgagaa acagaagcac
     3241 cggaagggta tcttttagat gcgacggtac atgatgtttt tataaatgaa tactccatag
     3301 aagaagtgac gttaaccatt gaaaacatcc ttgatacggg tggtgttgaa ctaattaaac
     3361 atgctgaagg cagtggtgaa gtccttcaag gagctgtttt tgagttacag aaccgagaag
     3421 gagaaacgct acaaaccggt ttaactacag gagaagatgg aaaactggca attgacggtt
     3481 tagcaccagg agcatatcag ctggtagaaa ctcaagcgcc aatagggtat gaattagatg
     3541 cgacaccaat cgagtttgag atcgaaagaa gtcagacagc ggtggtagaa ttgacgaaag
     3601 aaaaccgact tacaccaggc ggtgtcgtgt tgacaaagat cgatgaccaa agcggggaaa
     3661 tcctgcaagg agctgttttt gagttacaga accgagaagg agaaacgcta caaaccggtc
     3721 taactacagg agaagatgga aaactggcaa ttgacggttt agcaccagga gcatatcagc
     3781 tggtagaaac tcaagcgcca acggggtatg aattagatgc gacaccgatc gagtttgaga
     3841 tcgaaagaag tcagacagcg gtggtagaat tgacgaaaga aaaccgactt acaccaggcg
     3901 gtgtcgtgtt gacaaagatc gatgaccaaa gcggggaagt cctgcaagga gctgtttttg
     3961 agttacaaga tgccaatgga accgttctgc agtcaggtct aaccacagga gcagatggaa
     4021 aactggcgat tgacggctta gcaccaggag tgtatcagct ggtagaaacc caagcgccaa
     4081 cggggtatga attggatgcg acaccaatcg agtttgagat cgaaagaagt cagacagcgg
     4141 tggtagaatt gacgaaagaa aatcgactta caccaggcgg tgtcgtgttg acaaagatcg
     4201 atgaccaaag cggagaagtc ctgcaaggag ctgtttttga gttacaagat gccaatggaa
     4261 ccgttctgca gtcaggtcta accacaggag cagatggaaa actggcgata gatggcttag
     4321 caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa ttggatgcga
     4381 caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta acaaaagaga
     4441 atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc agtggaaata
     4501 agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca atattgggtg
     4561 tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga gctaaaataa
     4621 aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa tggaaactac
     4681 cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg ttgaacccaa
     4741 ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata aacctttgta
     4801 gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt gagtttcgca
     4861 gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct ttctacaaat
     4921 aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca ataaggagaa
     4981 aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa aggttacgcc
     5041 tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc atttggtaga
     5101 acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa ttctatttct
     5161 ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg tgtaggtaaa
     5221 ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt ttatcctcaa
     5281 cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc ttttggaaga
     5341 ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca aagataccta
     5401 acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag tgattttatg
     5461 tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag cgttccaatg
     5521 gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca ttggcatatg
     5581 attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca ccattaattt
     5641 cctatttcca tctaccacag cagaaccaca atttctacag acacatggag cagggaagag
     5701 aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga cctctgtaat
     5761 catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca taaagcatat
     5821 cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga tatgtttttt
     5881 tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat atgatgttcc
     5941 atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag aaaggacgac
     6001 aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa caaaaaatac
     6061 gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt ttacgatcat
     6121 tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta tttgaatcga
     6181 agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt tttgttgact
     6241 ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc tcactattag
     6301 tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt cattttattt
     6361 tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat gcttctttgt
     6421 acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg aacaagaatt
     6481 tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca gttctccttt
     6541 acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt tttttgaaag
     6601 gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt agtcttttga
     6661 tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt gcattttctt
     6721 ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca gtagaccgaa
     6781 tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt attcctttta
     6841 actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat agatataaaa
     6901 cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg gttccaggag
     6961 cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc atgtatattg
     7021 gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat cattagatga
     7081 gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt gctatcattc
     7141 catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt aatttatact
     7201 ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt tcgattgctt
     7261 cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt ggaaagtaat
     7321 agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt ctttcgacca
     7381 tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata ttcactgtga
     7441 tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt gccagaaatt
     7501 attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat attttgaata
     7561 gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg atttatgaaa
     7621 aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc aaagacaacg
     7681 gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc tacagaaggc
     7741 gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca aacgacgatg
     7801 tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt tagtatccct
     7861 ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt gatcgaagag
     7921 aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg ctgttgagat
     7981 atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct gaattgttat
     8041 tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta gaaaaaaacg
     8101 gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta caaagactga
     8161 catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga gaaccgcaag
     8221 gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt ttggaagatg
     8281 ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc agtttgacaa
     8341 aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc gtttctaagc
     8401 atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt atcatggata
     8461 ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa agccgttttt
     8521 tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca agagaagcta
     8581 agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct ttggggatca
     8641 aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa gtttctaagc
     8701 gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc gcaggtgtat
     8761 cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag tcgaatcaca
     8821 aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg aaaagattgt
     8881 ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga ctactcgatc
     8941 atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac ccattgactg
     9001 aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat ttcagcaaca
     9061 tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg gctttgcatc
     9121 tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg attatctgtg
     9181 caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa ttcggtaaaa
     9241 atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact ttaaaaggaa
     9301 tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt ccagttttac
     9361 atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta gttggagaat
     9421 ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag caaaaaaagc
     9481 agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac gaaccgaaaa
     9541 aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa gaaggatata
     9601 taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc atattcagtg
     9661 atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag gtagccgttg
     9721 cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt gtttttttag
     9781 tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca atcgttaggt
     9841 tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt gagaatttca
     9901 gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa aaaataaaca
     9961 gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat gcacggacac
    10021 tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta gaagagaagt
    10081 tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa cttctgaaag
    10141 aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca caagatcatc
    10201 tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg tatcaaaaaa
    10261 ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc gaattagaaa
    10321 aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta cgttacctga
    10381 ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca gctaaaagtt
    10441 taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta ggagaacaag
    10501 taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat tttttgttcc
    10561 agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt acattcttgt
    10621 atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt gttgtaagcg
    10681 gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc aaccaagacc
    10741 aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag gagaataact
    10801 aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg cgcaacttaa
    10861 aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga ttgtaggctc
    10921 gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag gattttttaa
    10981 tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga cgatggggat
    11041 tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg aacgagatga
    11101 acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg cattctttat
    11161 gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt ctatcaatga
    11221 gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt taggaacttc
    11281 aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt gtatgtgtgt
    11341 caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt ctcgttcttt
    11401 cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca atggattatt
    11461 agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt ttgttacgca
    11521 gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg cattgtggat
    11581 cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg tattagctaa
    11641 tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca acaattctta
    11701 tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg cattcctcgc
    11761 gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat tattcaatat
    11821 caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag cggttccgtt
    11881 tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc tggaaatcgt
    11941 caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag catttgttgg
    12001 tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca tcgcattcat
    12061 tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag aaagagaaaa
    12121 ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg aactgtgaca
    12181 aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata aataccatta
    12241 ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat ctacaccatt
    12301 gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt gcggagcgct
    12361 ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa cgatcaaatc
    12421 tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt caaatgctgg
    12481 gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc caaaatcatg
    12541 tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac ggccatctgg
    12601 atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat gtgcatagtc
    12661 cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg gtacacgagc
    12721 ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg cggcaatgat
    12781 cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat aagcagtatc
    12841 gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac ctttatctga
    12901 acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg gaatctggaa
    12961 tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta aaacaactgt
    13021 ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg tcggatgttc
    13081 gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta aagtcaagcc
    13141 gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat gagtcatacc
    13201 attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg ttgtcaaatg
    13261 ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca atgctgtttt
    13321 tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg ttttaccttt
    13381 tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa aagccatagc
    13441 aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg aattttcgta
    13501 tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca aatattcttc
    13561 tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc ttgaatcata
    13621 acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc ttgttgaaac
    13681 aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta aagagaagct
    13741 cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac tctttttaat
    13801 atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctc gacacagggg
    13861 acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg agaacgaata
    13921 gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta ttggtaagta
    13981 aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc ggtatcgtta
    14041 taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg aacaaaagac
    14101 ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga acaatctcta
    14161 aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat tttcctaata
    14221 ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact gagcgttgga
    14281 tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa gctatatcgt
    14341 gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa aatataacgg
    14401 gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca taaaaggctg
    14461 tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat tgttcttata
    14521 aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat agtaatgttt
    14581 tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta gatcaattaa
    14641 caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat atcatttcaa
    14701 gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa aatgctaagg
    14761 ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta ctgatctcta
    14821 cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca ttgaatgaat
    14881 atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg ttaggcctgt
    14941 tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct ggtggcggag
    15001 aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt ccttgtatga
    15061 gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg ctgtcgcaga
    15121 ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc atcatgaaag
    15181 tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca tttatcggta
    15241 tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg tatacatcgg
    15301 cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg ttgttagata
    15361 ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc ttcaaattag
    15421 tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc aatgcacagt
    15481 tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg gtagttattg
    15541 cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct gttacgttga
    15601 aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt tcatttttta
    15661 tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca ttattttata
    15721 caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga ctcttcctag
    15781 gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag gcggcaatta
    15841 attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc attcgtttat
    15901 ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct tgtaccttga
    15961 tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg cgcaggacat
    16021 tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca aagatgattt
    16081 ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt cttgtagtag
    16141 caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg gcagaaagac
    16201 tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa gtgaatcaga
    16261 agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat aagtttttag
    16321 cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa ggaacgatcg
    16381 aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc gaagatgcgg
    16441 ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt ttgcataaac
    16501 ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc gatttattgg
    16561 aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa gacacagagg
    16621 gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca cctaaaaaac
    16681 atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa gatatccgtt
    16741 tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca aaactccaaa
    16801 ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg catgaaggaa
    16861 agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta tatttaaaaa
    16921 gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa tatcgaccca
    16981 tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag ctaaaagaat
    17041 aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact ttcggatggc
    17101 ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc gtttgttcgg
    17161 aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg actcagagtt
    17221 agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc acaacctctg
    17281 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg
    17341 aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc
    17401 ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta
    17461 taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat
    17521 cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg
    17581 ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat
    17641 tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg
    17701 gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt
    17761 ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt
    17821 atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct
    17881 tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa
    17941 ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa
    18001 ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac
    18061 agcattattt actttgcatc tgttatggga atcgatccga cttattatga agctgctatg
    18121 gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca
    18181 ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg
    18241 ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg
    18301 tatatctaca atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt
    18361 taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt
    18421 gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag
    18481 tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg attgcactct
    18541 ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt
    18601 ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata
    18661 cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg
    18721 ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt
    18781 cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt
    18841 tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata
    18901 cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca
    18961 cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta
    19021 gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa
    19081 tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc
    19141 aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat
    19201 atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag
    19261 gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc
    19321 catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa
    19381 acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc
    19441 tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct
    19501 aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa
    19561 ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt
    19621 gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc
    19681 atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa
    19741 aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat
    19801 gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca
    19861 gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa
    19921 tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa
    19981 ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa
    20041 gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat
    20101 gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg
    20161 catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat
    20221 ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc
    20281 gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca
    20341 ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat
    20401 aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg
    20461 cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc
    20521 aaattgctag atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac
    20581 aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg
    20641 attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa
    20701 acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg
    20761 actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat
    20821 gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga
    20881 ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt
    20941 gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat
    21001 tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg
    21061 gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt
    21121 tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat
    21181 aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag gttttggaag
    21241 agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat
    21301 ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt
    21361 atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa
    21421 gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact gctttattga
    21481 gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct attgatttta
    21541 ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata
    21601 gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca
    21661 gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc
    21721 aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga
    21781 tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg
    21841 attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg
    21901 atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa
    21961 aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat
    22021 aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa
    22081 aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag
    22141 caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat
    22201 tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct
    22261 tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt
    22321 tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt
    22381 caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta
    22441 tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa
    22501 gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg
    22561 ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat
    22621 cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc
    22681 ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca
    22741 ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca
    22801 gggaatttag agacgcggat cgacataaca aacacggaat atgaactgag ggaactttca
    22861 atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta
    22921 gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt
    22981 ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta
    23041 gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg
    23101 atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt
    23161 tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta
    23221 cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat tgattttact
    23281 cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc
    23341 atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg
    23401 tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta
    23461 cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg
    23521 accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg
    23581 aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa
    23641 tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg
    23701 atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg
    23761 ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt
    23821 atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt
    23881 tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa
    23941 aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg
    24001 atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat
    24061 tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac
    24121 aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg
    24181 cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc
    24241 tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag
    24301 cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat gttttttacg
    24361 cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc
    24421 aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt
    24481 tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt atggagattc
    24541 aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc
    24601 aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga
    24661 gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc
    24721 aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag
    24781 gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta
    24841 tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg
    24901 agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat
    24961 cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa
    25021 ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa
    25081 ggcagctttt tcatgacctc tcgataaacg gagttcaaaa gccagtttct cggtattaag
    25141 ccaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa tactcgaaac
    25201 taaacagctt tttcacgacc tctccactaa acggtgttca atcagctgac cctcggtatt
    25261 agctcaaaaa atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg
    25321 tcagaacgct gatttcacaa cctctcaata aacggtgttc aaaagcttgc atctgcggta
    25381 ataagaccaa ccaaacgaaa aatatgagga tctatttttg cttggctgtt cttattactt
    25441 gatgcaggac agctttttca caacctcttg ttaattatag tttgataagg aaaaaggcaa
    25501 atcttttgct tgatatttct tagggtgagg gatgatccct aattggtaag agatcgtgcc
    25561 gcctttcagc aaatcttgat gggtaaaata taatttcgta tattctctgt ccatccgttt
    25621 gattttatgg ataaattgtt gctgcggccg attaggcgtt gcttcaatcg tcaattcctt
    25681 tccatttgac aaaagcaaaa cagccttgtc aattaatggc ataccgataa catattcacc
    25741 ggttccaggc gtgacaggat aaaatcccag actattgaag atataccaag cggccatact
    25801 tccattgtcc tcatctccag ggtaacccat tggagaatcg tcgaaggtct gagtcatcaa
    25861 ctgtttaagc agtggctgtg ccatttcagg ttgtcctata taactgaaca agtaaggata
    25921 gtgaaaactt ggctggtttg aaatagctaa ttgtccaaat tctatcgcgg ccatctcact
    25981 catttcgtgg atctcaaaac catatcctcc tgggtcaaac gttgggggct gattgcacag
    26041 ctcaatgagt ttttctttat atctctcact gccaccatat tttccgatca gtcctgaaaa
    26101 atcgtgataa acagcaaagc tgctttgcca agcacttcct tctgcataat cttgtcccca
    26161 acgattagct gaaaatggtt ctctgaactt gccatcttta tcttttgaac gcataaatcc
    26221 tgactcatag tcaaagatat tttgatagtt caatgattgc tgctgatatt tcttcgtcat
    26281 ttcctcttct tctagtacct tggcaacttg gctaatacaa aaatcgctat aagcatagtc
    26341 taatgtgtga ttgactgatt cgtggtagtg attaggaaca tagccatatg tcaagtaatc
    26401 ttctgttcct tggcgtccat aatttggatg tttgctttga accgtagcac ctttttccat
    26461 tgccgttaga aattcaggca tcaagtctcg tcggatatct tttacagcag catctgcaat
    26521 gaccgcatcg atcaatgtcc caggcataag tccacgttca tcaggagaca gccattttgg
    26581 caaatagcca ctttctctat agctgttcaa aaaaccttcc agcatttctt cgtatttttc
    26641 tacagcaatc aaagaataaa gaggataaac cgttttatac gtatcccaaa acccattatt
    26701 tgtatagaga acaccaggtt ttatagatcg actagtcgta tcatagtgga ttttttgccc
    26761 actaggatca atttcataaa atgtttgtgg aaataaaaac acccgatata aattatgata
    26821 aaacgtccgc aattgttgtt gatcatggtg tgtgagctgt atacgatcaa aataggactg
    26881 ccattgttct tgactgtgag caaggtaagt ctctttgctt tggttttgtt ctagttccag
    26941 gttataagct gcttgttccg agctgataaa agatgtccca aatcggatag tttgtttttt
    27001 tgtctgttca aaatacaacg ggatcgagtc gctatttttg ttggaaacag ctaactcatc
    27061 aattgttaaa ggatattcaa atctcaatat aaaataaaaa gaaaaatcag gatcttctga
    27121 accagcaaaa tgtgtgattt ttccttccac tgtgtaagga tctataacaa agagctgatg
    27181 gcgtcccgga aaggataaaa gtaaaccgct gtcatttttg ctgtaatcaa tagataagat
    27241 tccgccatac atgctaggta tcaatgtaga acagatgcca taacgtaatt gacagatcga
    27301 cagatgagtc gggcaaaata tactttcttc tgggcggtaa gaactttgag catggaaaag
    27361 ggtgttctcc tgtagttttc cattgatcgg cgtaaacagc atatgactga aatcacccat
    27421 ccaaggactt ggttgatggg taagacggta gccttgaaaa acgcggtcat caggatgaaa
    27481 ccaccagctg ccgttttgat ccgtagtttg cggtgcaaaa taattcatcc caaaaggaac
    27541 gccagtataa ggctggcagt tcccattaga gaaatttggc tgattcgctg ttgcatgtct
    27601 agtatctatt tgaccgatat tcattttatt tctcctttgt tgattgtctt ttgatgaatg
    27661 aaactggaaa tttactatcg gatggtcgat cttttgtctc taaccaagtg accaaagcat
    27721 ctcccgcaag cttacctaac tgttcaaaat cttgtgcgat ggttgtcagt ggcgggtcaa
    27781 caagagctgc tgcttggata tcatcaaatc cgataattga aaaatcttca gggatatgat
    27841 aaccatgctg tctcaagaga ttcatggtct ggatagcgac aacatcgttt tcacaaacaa
    27901 aagcagttac ttcatactta gtgatataag aaagcaattc atctaatgtc gcgcttttgt
    27961 catccagtga agtgtggaaa gtcaaaccag ctttgtccaa tgcttcaagg taaccaaggt
    28021 aacgctgtcg aacggattgt ggatgagtct gcgtaccaaa caaataagcg atttttgaat
    28081 gtccctgttc gatcaaataa gaggcagcaa gcgcaccgcc ttggaaatta tcggaaagaa
    28141 cggtaggaaa aggtaactca tagatttttt tatctaatat gacgacaggg aattctttca
    28201 atgaaagctc aaaaagtaag tcaagatgtt gttctgtatc aagagcataa taaatcaatc
    28261 cgtcaaattc attgattagc tcttttgctg ttttattttg tagaaagtca agcgtcgtca
    28321 tcatgacatc aatctgttta tcttgcatca cagggttcaa tcccttgcta aaatcaccaa
    28381 cagacaaatc agcgacatat ggaagaagaa acagaatccg tcgtgttttt gtaggagaag
    28441 caagaatctg ttccggagaa gcggatttaa cgaaactgcc tttcccgcga acgcgataaa
    28501 taagtcctaa ttgctctaat tcagtcaatg cacgtttgga agtgattctg cttactttat
    28561 aggtacgtga aagttctttt tcagttggta gttggctgtc tacaggtaat tcaccagtta
    28621 aaatagccgt ttgcaaatca ttaaatattg tctggtacaa tggttgagtc atataaacac
    28681 ctcttaatga tatatcatat tcaaataata ccataatgga tgaaaaagta aataaaaaga
    28741 tattttttga aagcgtagac ataattggct gtatgaagta taatctttaa agatatatca
    28801 aatagaaaga aggttttatt agtggtttat aaagatatac caaaatcggt gcagaaattt
    28861 atggaagata tcacacaaaa atgtggggat atccatgcgg actgggcaaa gaattttaca
    28921 gcagcatttg ccaatactct gttgacaaca gtcaaaaagc aaaatgacgg aaccaccttt
    28981 ttattgacgg gagacattcc agcgatgtgg ttaagagact ctactgcaca agttcgtcct
    29041 tatttagtga ttgcaaaaga agatgaggaa ttagctgaca tgatcagcgg gttagtcaaa
    29101 aagcaatttt tttatatcaa tatcgatcca tatgcgaatg ccttcaacga agaagctaac
    29161 ggtgctggcc accaaacaga tcatacaaaa atgaacgatt ggatctggga aagaaaatat
    29221 gaaattgatt ctttatgcta cccaatacag ttggcctatc ttctttacaa aaatacagga
    29281 aaaactgatc aattcaatca atcttttatt gaaggtatca aaaaaatctt ggacgtgttc
    29341 aaaacagagc aagatcatga acactctcct tatacatttg tacgagacac gacaagaaaa
    29401 gaagatacgc tgatcaatga cgggcgaggt gcaaaaacag cttttacagg aatgacatgg
    29461 tctggtttcc gtcctagcga tgatgcttgc cgttatggat atctggtacc atccaatatg
    29521 tttgcggtgg tcgtgttagg ttatgtggaa gaaatttttc aaacgatact cgatgataaa
    29581 gaaattgtac agcaaacaca gaaattgaag aaagagatcc aagaggggat tcagaatcac
    29641 ggacgaacgg ttaatcaaaa agggcaaacg atttatgcat atgaagtaga tggaatggga
    29701 aacgccacag tcatggatga cagtaatgtg cccaatctga tcgccgcacc ttacttggga
    29761 tattgttctg aacaagagga agattatctg caaacaagaa gaacattgtt gagtaaagaa
    29821 aatccatatt tctatgaagg aaagtacgca aaaggaattg gatcctccca tacgccagaa
    29881 aaatacgttt ggccgattgc attagctatg gaaggaatga caacgtcaga caaatctgaa
    29941 aaagagcgga tattgaattt acttgtagca actgacgcag gaacacacct tatgcacgaa
    30001 ggattcgatg tgaacaatcc tgaaaattat acgagagaat ggttttcttg ggcaaatatg
    30061 atgttttgcg aattagtcat ggattacttt gatattagag tagaaaagta ggaggtatcg
    30121 taaatgaaaa agaaagtata tatcatttct catagccact gggatcgtga atggtacatg
    30181 gcttacgaac agcatcacat gcgactgatt gaattgatag acgatctact agagctattt
    30241 gaagttgatc cgtccttcaa cagttttcat ttggatgggc aaacgattat tttagatgac
    30301 tatcttcaag taaggccaga aaaaagacag gcaatccaac aagcaatcaa tgaaggaaaa
    30361 ttgagaattg gaccatttta tatcttgcaa gatgattttc ttatcagctc ggaatcgaac
    30421 gtgagaaata tgctgattgg catggaagaa agccgtaaat ggggaacccc tgtcatgtta
    30481 ggctattttc ccgatacatt tggaaatatg ggacagacgc ctcaattaat gaaacaagct
    30541 ggtatttcag cagcagcgtt tggccgtggt gtaaaaccaa ttggctttga taatcaagtt
    30601 ttggaagcag aaaactattc ttcccaatac tcagagatgt ggtggaaagg ccctgatcaa
    30661 acagcaatct ttggtctgct atttgccaat tggtatagca acgggaatga gattccagta
    30721 gaaaaagaag cagctcttgc cttttggaag caaaaattag cggatgcaga acaatatgcc
    30781 tctacgaacc acttattgat gatgaatggt gttgatcatc aaccggtaca aaaagatata
    30841 tcaaaagcaa tccatctcgc aaatgaactg tttccagatt acgagtttat tcattctaat
    30901 tttactgact atctggaagc agttcaaaaa gatgtgccag aagacttagg ctcagtagaa
    30961 ggagaattga caagtcaaga aacggatggc tggtatactt tagccaatac agcatctgct
    31021 cgggtttact taaaacaatg gaataccaag gtccagcgac agctagaaaa tattacagaa
    31081 ccacttgcga cgatggctta tgaagttagt ggaaactatc cccatgatca attagattat
    31141 gcttggaaga cattaatgca aaatcatcct cacgacagta tttgcggttg ttcagtcgat
    31201 tctgtccatc gggaaatgat acctcgtttt gaaaaagcag atgaagttgg aaagtatctt
    31261 gcacaagatt ccttagaaca gctgaccgca gcaatcgata cgaccggatt cccaaaagac
    31321 agctttccat ttgtcattgt aaatactgca ggaatggata aaactggtga agcagaaata
    31381 accatcgaat tagaaagaaa aagattcgct gaaggaatac ctgaacaact atatcaagag
    31441 ttagaaaatt tgcctaaacg taagtatcat gtagaaacaa aaagtggggc cactatacca
    31501 gctattttat ctgaagaaac tgtccaattt ggttatgatt tgccgaaaga ccgtttccgt
    31561 gtgccatata tggcaagaat gatcaaagtg actttaccat tagaaaacat gccagcattt
    31621 tcttgggaaa cattcgcgct tgtagaaggg gaagcgaaag cagaagaaaa ggaaacaatg
    31681 attcatcaga gtggacgaat catcgaaaac ggccctctac atttgacgat tgaaaaaaat
    31741 ggtaccatta caatggaaga tcgcaaaaac aaacgaaagt tgaataatct gcatattttt
    31801 gaagatatcg gcgatatagg aaatgagtat atctttaaac aacctttctg tgatcagcca
    31861 atcctatcca gtaacaaaga aaactctgaa gtaaaagtat tagtagatac accggaaata
    31921 gcaaaaataa gtattctaca ggaaatggaa atccctgttt ctgcagacga gcgtttagaa
    31981 aaagaacagc agatggttgt ggaatttcgt tatcgaaaag cagaacgatc caaagaaaaa
    32041 cgaatccttc aaataaaaac aatcatgaca atcagaaaag atagtaaaaa aatcgacttt
    32101 gaaacgactc tcgacaatca gatgaaagat catcgcttac gtgtcttgtt tcctacaaag
    32161 ttacatgtgg aacatcacga agcagatagt atttttgaag tggtgaagcg cccaaaccat
    32221 gtgtctaaaa gttgggaaaa ccccacgaat ccgcaacatc agcaagcatt tgtcaatatt
    32281 catgatgaag agtacggtgt aacagtcgga aactttggtt tgaatgaata tgaagtaaca
    32341 gaagacggac aaatcgcagt gacgctactt cgcagtgtgg gagaactagg cgattgggga
    32401 tattttccaa caccagaagc acagtgtcta ggtgagcatc ggttcaatta cagcattgaa
    32461 ctacatggac cagaagaaaa attctctact tatttgcatg catacgcagc acagattcct
    32521 ttttccactc aacaaatcaa acatcatgaa ggaacattga tttcaaaaca acaatattta
    32581 acgataaaaa gtgaaacgtt tgcaattaca gctttgaaac gcagcaagtt tagtgataaa
    32641 gtagtagtaa gaggtttcaa tatgtctagt catttagaaa aattggaaat aactaaagac
    32701 aatggaaaaa ctgtcatatt gaatctgctt gaagaaccaa cgaagcaagc ggttgtccca
    32761 ataatccaac cttatgagat tcggacaatc ggattcgagg aggaaaacga atgtatggcg
    32821 gaatagaagc aggcggaaca aaatttgtgt gtgcggtttc agatggtgga gaaattttag
    32881 aaaaattaag tatccctacc acaacaccag aagaaacatt ggatcaggta tttgaatttt
    32941 ttgatcgtta tgaattgaag ggaatcggga ttggctcatt cggcccgatt ggtatcgaca
    33001 aaacaaaaga taattacgga tacgttttgg caactcccaa aaaagggtgg gcacaattcg
    33061 attttcttgg cacaatcaag gaacgctaca atattcctgc tgcttggaca acggatgtca
    33121 atgctgctgc atatggggaa ttgaagcaag gagcagcaca aggaaaaaat agttgtattt
    33181 accttaccgt gggaacagga atcggtgcag gtgtcgtgat caatgaagaa attttttcag
    33241 ggatcgcaca tcctgaaatg ggacatattt gggtgaaacg ccatccagat gaccattatg
    33301 aaggtacttg tccttatcat aaagattgtt tagaaggatt agcagcagga ccgtctattg
    33361 aggcacgcac agggatcaaa gggcaagatc tgccgcaaga tcatcctgtt tgggctattc
    33421 aggcttatta tattgctcaa gcactgatca actatacgtt gacgttagca cctgaaaaga
    33481 ttattttagg cggtggggta atgaatcagg atcatctcct tcaaaaaatc cgtcagcaat
    33541 ttgttgaatt gatgggtggt tatatggaaa cacctcaagt aagtgaatat atcgttcgtt
    33601 ggggactgcc gaatgaaagt gggattatcg gtagtttgct gttagcagaa cacgaacatc
    33661 aaaagtaagc agacaaatta cacaaaaaga acagagtctt atcattatct gctgataaga
    33721 ctctgttctt ttttatttct attcccataa tccaggtaaa gctttaccga ctattttttc
    33781 ttgagtaaat ggatggatca tgtgcagttc taatgcatgg agcatcagtc gttcagctgt
    33841 tctattttgg tagagagggt ctccaacgat cggatgtccc ttatgagcta aatgaactct
    33901 gatctggtgt gtacgtccgg tttccaaaac acaatagact tgtgtttgtt cagaaagtac
    33961 tttgttgaca gtgacatgtg tcaaggcgct ctgaccattt ttatcatcta cgaccctttt
    34021 tctccgatca tggcgatctc gtccaatttt attggtgatg gtcatatctt tggcaatttc
    34081 tccccatacg atggcttgat atctccgata gatcattttt tgttccaaca ttcttccaag
    34141 tatcggcaag acaaatggat tttttgcaaa taaaacggca ccgcttgttt ctttgtccaa
    34201 gcggtggaca acgtaaggtc gttgctgttt agtgtcgaga taagcagcta aatgattgag
    34261 taaggtactg ttttctgtcg gttcatttgg atgagtcttc attcctgcag gcttatccac
    34321 tataatcaaa tgctcatctt catataaaac acggatgtgc ttcgcatctc ctaattgaac
    34381 ttcttgataa ttataatctg tttcttcgaa taacaatgtg atctggtcat tgccctttac
    34441 ttcatgatgg aacaaagcag gttgttgatt gatccaaacg tttttacgta tacgcaaaaa
    34501 gtgtcgaacc tttctaggaa ctaaccattc cttttcaagc aagtcgcgaa ttgttgtttc
    34561 aggatggttc ataggtaaag tgatcgtgat tttcacaagt gtactccttt cagtgcaatt
    34621 gcagactaat tgtaccattt ttaaaacata tttaagaaaa aacttacatt tttctgttca
    34681 ttcttatgtt actctacttg cactaacgta aaaaaacata ttgaactaca acttaagcag
    34741 gaagaaaaca ctctttcact atggtaaaat aagacgattg aatcaaatag acaggagaag
    34801 aagcaaatgg attttcaaag cattttagaa aagataaaag ctgctttgat cagtttttgg
    34861 cgttggatca gaccatatct cggacaattc catcgatggc gaaagcgcat ctggaaaaaa
    34921 tatcatgtga acaaattgat tttgctgatt ggtttgatct gtgtcttagt gacaagtatc
    34981 tacctcttta ttttagcaaa acaagcaaat gtcgaaacgt taaaatcagg attaagccaa
    35041 tcgacagtca tttatgataa gaaaaacgaa gaagctggca cactgtacgc ccaaaaagga
    35101 acatatgtag aattaaacgc gatttctccc tatatccaag atgcagtaat ctcgacagaa
    35161 gataggaatt tttacgagca tcacgggttt gatatcaaag ggattgcccg tgcagcagtc
    35221 cgaatggtct tgtctggcgg aacatcaggg ggcggaggaa gtacgatcac tcagcagctt
    35281 gccaaaaatg cgtatctgac attagaccaa acgtttgatc gaaaagcgaa ggaattattt
    35341 ttggcaatcg agatcgaaaa gaagtacagc aaagaagaaa tcttgaccat gtatcttaac
    35401 aatgcctatt ttggcaacgg ggtatggggt gtccaagacg cttccagaaa gtattttgga
    35461 gtagatgcaa cgaatgttac gctgtctgag gctgctactt tggcaggaat gctgaaaggt
    35521 cctggtattt ataatccgat cgatcaccca aaaaatgcga atgatcggaa aaacacagta
    35581 ctaagtgtaa tggaagaaaa cggaaaaatc acaaaagaac aagaacaagc agaaagccag
    35641 acagatatca gcgcgtatct taatgacaca tacgataatt cagattctgg ttatcgttat
    35701 ccttattatt ttgatgcagt gatcgatgaa gcagttaacg agtatggtct tgatgaagaa
    35761 gatgtcatga ataaaggcta taaaatctat acagctttag atcaggatta tcaaaagcaa
    35821 ttagaagcta cttatcaaaa cagtgcatta ttcccagcaa atgcttcaga tggtgccatg
    35881 gtccaatctg cttcagtagc tttagatccg aatacaggtg gtgtacaagc actagtagga
    35941 cgaagaggag atcatgtctt tagaggattc agctttgcta cacagatgcg tcgttcacca
    36001 ggatcaacga tcaaaccatt aagcgtatat acaccagcac tggaagctgg atacaagcct
    36061 tccagtatct tgaaagacga accgcaaagt tattacgatg cgcataattt tgacgggaca
    36121 taccaaggag aagtaccgat gtatcaagct gttgcacaaa gtttgaattt gcctgctgta
    36181 tggcttttgc atgagatcgg tttacaaaaa ggatatgata aagcagaaga attcggcctg
    36241 cctttagcaa aatctgataa gtattatggc ttagcccttg gtggactgga aaaaggagta
    36301 tctccattga tcatggcggg tgcttacagt gcatttgcca atgatggtca gatgtatacg
    36361 ccgcatctta tcacgaaaat cgttgattcg actggagcgg tgatcgtcga taacacaaac
    36421 aaaaaaccaa aacaggtcat atcaaaggaa acagccgatg aaatgaccag catgcttctt
    36481 ggtacatttt caaatggaac agcggctgct gcgaatcctg caggttatac gatagcaggg
    36541 aaaacaggaa ccacagaaac taatttcgat gccacaaaag caaatgacca atggatgatc
    36601 ggttacacgc cagacgtagt gatttcgaca tggatgggtt acgaagaatc cagcaagctg
    36661 cactatttgg aagggacaag tggaaatgtt gtcgggaaag tcttcaaatc agcggcagaa
    36721 ggcatccttc cgtatacttc aaaaacagga tttagtgtag cagatgccta tgccacagga
    36781 ggacaagtgg tagctgcaga tcaagttgga aacacaggaa attctaatgg agagtctgga
    36841 aattggcaag ataacttgaa tcaatatggt gaacaagcaa aagaagggct gaaaaacttc
    36901 ggcgatatgg tgaaagaagg cgttcaggga attggtgaag cggctaaaga cctctggcgg
    36961 aaatatcaag gagagtagcc cctttgaaaa cgaataaccc catgttacaa tagagagtaa
    37021 ctactaaaag aaaagagggc ataacatgtc aaacatctac gatagcgcga atcaaatcga
    37081 acgtgagatc cgtgaattac cagaattcaa agcgttgaaa gaagcgtacg agaaagtaca
    37141 agcaaacgaa gaagcacaca aactgttcaa agacttccaa gcgatgcaaa tcgaattaca
    37201 agaaaaacaa atgagtggac aagagttcag cgacgaagat gcaatgaaag cacaagaaat
    37261 ggctgtgaaa atccaatcag aagaagtaat caatgacttg atgcaaaaag agcaaggctt
    37321 cagtacaatc atcaatgact tgaaccgtat catcatgaca ccagttcgcg acctttacag
    37381 cgaataaaac tattttatga aacaacagag ggtgtgaaag agacgttcgg ccttgagcga
    37441 tgacaaaatt gcttcttatt gccgataggc tgtctcttga acaccattta ccaagaggtc
    37501 gtgagcctac cgtttaactc tgagttacaa ggaacaattt caaaaaacag cttctcatgt
    37561 ttgatgacta ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcagaaa
    37621 taggagctac gacaaaagtc ttgtcatagc tcctattttt tgtcattcat ttttcaagcg
    37681 gtagaaatag cctttttgag ctaacagttt ttcgtaagta cctatttcaa cgatttttcc
    37741 ttcatcgatc acaacgattt tatctgcctc tttgatcgtt gaaagccgat gagccacaat
    37801 aaaagtagtg ggtgtttctg tcaggtaatt cagggcttgc tggatttttt tctctgactg
    37861 gttatctaaa gctgaggttg cttcatctag caaaatgatt tttggctgac gtatcaacgc
    37921 tcgtgcaatg gagatacatt gcttttggcc accagacagc ttgttgccat gttcgcctac
    37981 taaggtatcc agaccatcag gaagctggtc aatcagtgaa gaaagttgcg cagcttcgat
    38041 gacttcatct aaacgttctt ttgatacgtt tttcaagcca taagtgatgt tctctttgat
    38101 cgaagcggag aacaacaaag tggtttgagg aacaacggct aaataatttc gcacacttcg
    38161 catatccagt tcatccaaag gctgatcatc tagaaaaatt tttcctgaag tcggctggat
    38221 aaatccaatc aataagttaa ggagtgtaga ttttccagag ccagatggcc caacaaaagc
    38281 aattgtttct ccagctttca cctctagaga aaaatgatcg ataatcggtt tgagtgagtc
    38341 cggataaaga aaagaaacat cctcaaaacg aaaatttcca tccaaatgtt ggattttttt
    38401 cttaccgtga taagtttcta catcgccagc aatcaacagt tcgccaatag aagtgataga
    38461 ttccatgcct tttgaaatcg taggaatcag cgtgatcatt ccagaaacgg aattgacgat
    38521 cgtcgtgaaa tacgtctgat acatcacgac atcgccgggt tggatgcgcc cttgtaaagc
    38581 taatatccca gtgaaaatca aacaaatcag ctgaaatatc tgaaaactag cccagctgac
    38641 tgatccgaaa acagattgca ggatatctaa gtggaatcct ttttcataga tttgagtaaa
    38701 atgcttattt aagcgagttg tttcgtgttt ttctaaccca tgagctcgag caatcggtgc
    38761 taattcgatc acttcgatca cttttgcaga agtctcttcc atttcttgcc tgaagtctga
    38821 gttggactgt ctgatccttt tacggaaaaa gagagtaacg aatgcggcga gaggaacagt
    38881 caataagaaa aacatgaaaa caacaggact tttataaagc gtgataccta aagctacgaa
    38941 taaattgaca gcgatattta atccgctgac aaatacttga gaggacaacg tttggatagc
    39001 ttctacatca cggatgatct ttgactggat tcgtccagat tgcaattctt tttgataagg
    39061 aatagatagt tcttggattt tcttgataag ggtcgctctt agtccagctt ctacttgacg
    39121 gatcgaagtg ctatggtatt ttacatgaaa ataattcgtc aatatatttt gaccaatcag
    39181 acaagccatc aaaaaagcgt tgaaataaat ggcctgcaac tgagaagaat ctttcgatga
    39241 agctgcattg attacattgg caatagcgat cggcattaac caagatggaa gatgtttgat
    39301 aataaaaaag agtcctgaca tggcaaaacg ccaataattt ccctgataaa gatgcagcaa
    39361 tgtctgaaaa gcatttgcat gatatttttg ataacttttc tgtattcgtt cctccataat
    39421 cttctcctta acaatcttgg taaataaact gcaagtgttc tattgcttca ggaacataaa
    39481 tctcaggagt agcttcaaga gtggcgtaca ttagaggacg ctcatatttg atataatgca
    39541 gtaccgggcg gaaatctaaa cagcccttgc caacaggtac catgcggatc tttccatttt
    39601 ccatgatata gtctttgaga tgaagcgctg taatataacc atgtaaattg tccaaggcac
    39661 ctcggatcac tttttcctga tcagcatgat tttctttatg catcaaattc gcacaatcta
    39721 aaatgatttt taaatttggt gaagctactt catcgattaa acgtttagct aactgatagt
    39781 tatagattgg atgattgatc cctgcttcaa cagcaacagt tacgccaaaa ttttctgcat
    39841 gagcgactaa ttcaatgata gaatttctgg cgatctgata agcttcttca gtaaaatttt
    39901 cttttgtgta accgacagag acactaccgg tttccgtagc aatcatcttg gcttgatagg
    39961 cagagcacag agcaatttgc tgtttgaaag ttgccaatgc ttgtgcacga acttcttgat
    40021 ttttgctgga taggttgata tagcatccta aaatggaaag ttcgatattg tttttttgga
    40081 agtattcacc aaaataggca gcactcccaa gtgtcatgtc agttaattta agttgttcag
    40141 gaaatgcttt ttgtggtgca aattggatat gggacaagtg aagctgtgat actttttgaa
    40201 cgatttcttc acgatttcga acatctaaat cgtgggcacg gatgcctaaa tttaatggaa
    40261 acatctcaaa ctcctttttg taaataatta gcaagaggca gacgggattc ttttatcaat
    40321 tcaacaatta ttcgggcaac tttatcagcg ccttctggac taaagtgggt attgtctttt
    40381 agtccttctg ggtaattttt acaagtatca ggcataagat gcaggtagaa tttttttgtc
    40441 tcttcttttg agaaggtctg gaacaattct tgtgatcttg agaacacatc taaaagagga
    40501 atatggtgtt tctcagcaaa tgcacgcatc gcttttggat agtctccaag tatatctgga
    40561 ttcaatgttc cgttttctaa gtaatctcgt cgcgtcacag atgtcagtaa gaccatttgg
    40621 acgttcgctt tttttgcgca ttcaaaaaat atctgcagat tttttaaata gctaccgaag
    40681 ggaagcgtac ctctttcttc ttggattttc tggtcgttat gtccaaattg tacaaataag
    40741 aaatcattcg gctgaaactg tgaagctaga cgggctaacc ttccttcctg gataaatgat
    40801 ttggtgctgc gtccattctt tgcttggtta ttgatctgga tctcgggtga aaaataagca
    40861 gagatttttt ctccccaacc tgtctcggga cgcttgtttt catctttttc tgccgcagta
    40921 gaatcaccgg caatatggat agtaggcatg ttatctcttc tttcataaag gtttataggc
    40981 aatgaagcag aacagcaaga gaagaagacg gagcaa
//
LOCUS       DACDKJ010000018        40755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDKJ010000018 DACDKJ010000000
VERSION     DACDKJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.018"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            173..1309
                     /locus_tag="IV328_001100"
     CDS             173..1309
                     /locus_tag="IV328_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8937257.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="IV328_001101"
     CDS             1446..1790
                     /locus_tag="IV328_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8937258.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="IV328_001102"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="IV328_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8937259.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="IV328_001103"
     CDS             complement(3485..4084)
                     /locus_tag="IV328_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8937260.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="IV328_001104"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="IV328_001104"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8937261.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="IV328_001105"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="IV328_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8937262.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="IV328_001106"
     CDS             7290..8408
                     /locus_tag="IV328_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8937263.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="IV328_001107"
     CDS             complement(8468..9760)
                     /locus_tag="IV328_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8937264.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="IV328_001108"
     CDS             9910..10470
                     /locus_tag="IV328_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8937265.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="IV328_001109"
     CDS             10599..10976
                     /locus_tag="IV328_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8937266.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="IV328_001110"
     CDS             10909..12981
                     /locus_tag="IV328_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8937267.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="IV328_001111"
     CDS             complement(13192..14139)
                     /locus_tag="IV328_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8937268.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="IV328_001112"
     CDS             14385..14849
                     /locus_tag="IV328_001112"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8937269.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="IV328_001113"
     CDS             complement(14911..15954)
                     /locus_tag="IV328_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8937270.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="IV328_001114"
     CDS             complement(16331..17296)
                     /locus_tag="IV328_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8937271.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="IV328_001115"
     CDS             17530..18375
                     /locus_tag="IV328_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8937272.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="IV328_001116"
     CDS             18566..19753
                     /locus_tag="IV328_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8937273.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="IV328_001117"
     CDS             19759..20115
                     /locus_tag="IV328_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8937274.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="IV328_001118"
     CDS             20117..20455
                     /locus_tag="IV328_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8937275.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            20850..21884
                     /locus_tag="IV328_001119"
     CDS             20850..21884
                     /locus_tag="IV328_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8937276.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="IV328_001120"
     CDS             21877..22563
                     /locus_tag="IV328_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937277.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="IV328_001121"
     CDS             22587..23435
                     /locus_tag="IV328_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8937278.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="IV328_001122"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="IV328_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8937279.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="IV328_001123"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="IV328_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8937280.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="IV328_001124"
     CDS             25701..26936
                     /locus_tag="IV328_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8937281.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /locus_tag="IV328_001125"
     CDS             26923..27393
                     /locus_tag="IV328_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8937282.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="IV328_001126"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="IV328_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8937283.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="IV328_001127"
     CDS             complement(28884..30026)
                     /locus_tag="IV328_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8937284.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..30799)
                     /locus_tag="IV328_001128"
     CDS             complement(30065..30799)
                     /locus_tag="IV328_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8937285.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31031..31306
                     /locus_tag="IV328_001129"
     CDS             31031..31306
                     /locus_tag="IV328_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8937286.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            31404..33401
                     /locus_tag="IV328_001130"
     CDS             31404..33401
                     /locus_tag="IV328_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937287.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(33477..33812)
                     /locus_tag="IV328_001131"
     CDS             complement(33477..33812)
                     /locus_tag="IV328_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8937288.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            33939..34154
                     /locus_tag="IV328_001132"
     CDS             33939..34154
                     /locus_tag="IV328_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8937289.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            34191..34553
                     /locus_tag="IV328_001133"
     CDS             34191..34553
                     /locus_tag="IV328_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8937290.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            34599..34925
                     /locus_tag="IV328_001134"
     CDS             34599..34925
                     /locus_tag="IV328_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937291.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            35768..37591
                     /locus_tag="IV328_001135"
     CDS             35768..37591
                     /locus_tag="IV328_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8937292.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            37605..39014
                     /locus_tag="IV328_001136"
     CDS             37605..39014
                     /locus_tag="IV328_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8937293.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            39032..39868
                     /locus_tag="IV328_001137"
     CDS             39032..39868
                     /locus_tag="IV328_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8937294.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            39933..40715
                     /locus_tag="IV328_001138"
     CDS             39933..40715
                     /locus_tag="IV328_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937295.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
BASE COUNT        13340 a         7148 c         8583 g        11684 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    30181 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    30241 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    30301 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    30361 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    30421 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    30481 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    30541 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    30601 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    30661 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    30721 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    30781 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    30841 gtgttgcaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    30901 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    30961 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    31021 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    31081 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    31141 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    31201 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    31261 taaaaaatct gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga
    31321 ctaaaacata tagtaataaa gagtttgttt ttattatctg tatgttttag tctttaacag
    31381 aattttataa ggaggaaaca caaatggaaa catcaatgat taatattcca atttatgtag
    31441 atcaagaaaa attattagag aagccagtta caaaaaaaga aaaaactgat gtaagagata
    31501 ggtgtttaga taactataga gaagttacaa gagatgagtt tgtagagata ctaaattcat
    31561 cacaatcatt tattccaagc aagacaaaga ataaaggcaa tgacaaggag agttttgtgg
    31621 aaaccagagt aattattctg gatgtcgata acactgttaa agatgagaag gggaaggtag
    31681 tagatttgag taaagatgat agtagatact taagcgtaga aaaggtttta tctatcaatt
    31741 tagttcataa ttctgcgttt gcgatacaaa aaagtattcg gtactctgaa aatctagaaa
    31801 aatacaagat tgtctttcta ttaaaagaag caattactga ctatggtaaa atggttgctg
    31861 tttacgaata cttgaaaaag aacatccccg ggtgtgatga caatgtaaat gcttcgaata
    31921 gaatgttttt tggaggatat aagtcagatg cagtgataat aattaatgaa gacaacatgt
    31981 tagatattac tgagcttccg attgatttta ccatgacatg ttctaaaaat tgtcaaagtg
    32041 attcagggag agagtatgaa ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg
    32101 acgacaaaga agaaatgaaa cagtggttta aagactgcct gtttgattcc tccgacatgt
    32161 cttttaatga gatttatgaa agattgttaa ccattgatat gaataaatta cttaagtcaa
    32221 gtaaaaactt aagatgtctg tttcacaacg atcataatcc ctctgctagt atttttacca
    32281 gtaaaactgg gcattccatt ttttattgtc attccagtaa ttgtgatatc agtgaaaatt
    32341 ttattggagt tgtgatgcta attttaaaga aagacagtcg ggtagaaaca tttaattggc
    32401 tattagagaa cctagacttg tatcctacac attttcgaaa attgaaagag gaatcaaaag
    32461 tactttttga cactcttgaa tcaatgaaag acaaactttt caggacaata aggaattatc
    32521 tagaggaaat gcgacgtgtc tatgatgtct tgctttctga tgttaatttt tttgatgata
    32581 gtaaggaaag tgtgacttgt attttgtcgg gggaacaatt agctaaaaaa atgagttctt
    32641 actatggtaa aacttatgac atagataaat gtaataagtt actatcactt atgactttta
    32701 taggattgat aaaaaaatta gatgatgaag atgttccttt gaagatgctc gaatacctaa
    32761 ataggttaaa ggaagagaaa gcgttaggaa actttaatca taagagaagc aatgtctatc
    32821 gattagacgt ttttaatgca acttctgtga ttcaaaaatt gaatgaagat atccttccac
    32881 ttctggaaaa aattcatttt acatatcaat atttttctta tgaatgggtt aagatatgtt
    32941 ttaacgaaaa agaggctaaa agagtatttc ctcaaaattc ccgatcagaa ctcagcaagg
    33001 aaaaacaatt gattttagat gaagcacatg aagttttaca gggcttatcg tggcaaggga
    33061 catatctaat gacagagaaa gaacttatta acactgtttg tgaacgccta cgtaatgttg
    33121 gggataacag cgtgaaaaat gctttaataa aaaacagagg ctactttgtt aaacaaggat
    33181 tcactcttga acgagcttct aaggatatta agaaactata caatgtgaaa gcaagtagta
    33241 gttttttaat atatttagcg aaaggggcag ggatagatga aacaataaaa gaattgatat
    33301 tatcaggagc tgggatagtt cctctgtatc aatttacaag tgtaacaaga gatgatggac
    33361 ggagagtaag gaagagaaaa gacccaattg tattaaacta gacaggttat ccatagctct
    33421 taatcttatt tcatctcaat acacccaccg ttaaagttcg gtgggttatt tatttttcaa
    33481 tctttcaata gctgattgcg ttcattttct tgccttttta atagcttcat atagtcaatt
    33541 gttcgaactg catcttcagt gctaagagaa cgatagagag ccaaaaactc ttcctcataa
    33601 tctacgacaa cattgtctag aaaatggctt acaggggttc ctagggctct agaaagagct
    33661 gttaagctat cgaggttagg ctctgttaca gaattttccc atcttgctac tgcctgttgt
    33721 gtagagaaga ttttttcagc taattctttt tgtgtccacc cgagagcttt tcgcttttct
    33781 ctaatgacct ttgcatattg tttctgtttc attaatacac ccccatctca gtatagtaca
    33841 acaagttgtt gtatttgtaa tggtatttga agttttttat attttatgag ttgaaataca
    33901 tcatattgtt gtatataata aaacaggagg ggagaatcat gaaaaaagta agtaatctca
    33961 gccgatacag aaaagaaaaa ggactttctc aaacggagtt agcaaaaaaa atgaatgtta
    34021 cacagcaatg tgtaagttcg tggcaaacag ggagaacgat tcctaaaccc tatcaaatga
    34081 agatgctttc tgagatatta agtgtaccaa tgaatgagct gttttctgat gtatttaata
    34141 aagtaaacag ttaactaata agcaattgaa tgattttagg aggagaagac atgtattgta
    34201 gaaaatgtgg caaagaaata acagatgaaa gtaagtattg ccagttttgt ggagcactac
    34261 aagaaacaga tgagacaacc ggaacagaca cagtaaagaa aagtactgag cagacaatta
    34321 atgaaatgaa ggaacaaatc gtacatggac aagagacagg gaagctgtat ttgattattg
    34381 gttggatttc tatggtagta tcgttagcat ttattcctgt tgtatttggt gctgttgctg
    34441 ttattatggg ctacttatat cgagaaaaag atgagaaaat gggtacgatt ttgatgatag
    34501 caggggtagc tggagcaatc tttggtgtac ttttagggat gtcaatgtac tgataagagg
    34561 gataagagca aattattttg gagataggag ataaaaagtt gaataaagta aatatgaaca
    34621 agaagtttgt cattataggt attgttgtct tggctattat aggtattatt ttttcaaaaa
    34681 tgacaggtac atctggatta gagaagtcat caaaaccatt ggtaacacag attttacaag
    34741 aacagtacaa cttaaatcga tcatgtgatg atgtgacgat tactcatgaa aatggagata
    34801 acacataccg ggctaaagct atattagaca atggaagtgc tatcaacata aatattgagt
    34861 actatccaaa gaaagaccgt atatatgtgg agataccata tgctgaggtg ttaatgttaa
    34921 attgatttaa atgtattatg cataccgtcc aaacaggaac tcccaaaggt gtaaattcac
    34981 tagggagttt ttatagtttc ctataaatta aatactaata caggtgacaa aaaaatatta
    35041 caatgattgt cgggaatttt tatgttattc actgttataa caatataaaa gaaatgaaaa
    35101 agaaaaaatt attatggata tgtttggtat ttttattgat aattggtgga attgaaatac
    35161 ttactacatg tagattagaa ataaatcagt ttaaacattt gctagttaat ttttaagaat
    35221 actagcccaa atcattctat cgataccttt atatctcgga atgattgaaa ttggctattg
    35281 cttactgatt tttaccaaaa aaacaagtat cactattgtt gcctatattg ttggaatgct
    35341 gtttattcca tcctttactc accaaattca tcaatttttc ccaagagcag aattgttgaa
    35401 gttgtgtgat ccgttatatg catttttgat gctttcacga ttttgggaat ttcccgctgg
    35461 ttatgtagga atggttttac tggtatagtt aatattagat atacttattt tagcagtaag
    35521 tatacgcaga tataccctag ttgatgtgat ctaactgtta tattacccta tacggtcaaa
    35581 cgaaaagata aaaaattaaa ttccctgttg attgcgctat cataatgtgc tattattgga
    35641 ttacagcaaa ataacgttat tggcatgtta ctgattatgc aggcaagacc aagacaaact
    35701 aacacttcaa tgttgattgg ggcgtggttt gacttggtct tttttactgc tttaaggaga
    35761 gagaataatg gattacagag aagtaggaag aaatgtattg caacaggttg ggggaaaaga
    35821 aaatgtggtc agcttaaccc attgtgccac cagactgcgt tttgaactca aagataaaag
    35881 gaaagccgat acgaaagcct tagaaaaaac acttggtgta attagtgtgg tggatagcgg
    35941 tggacaatac caagtggtga ttggcaatga agtccaaaca gccttcaagg aaattcaaaa
    36001 ggaagtggga acttcaatta aaaagggccc ctcttcagac aatcaagaaa gtattatttc
    36061 aagattgatt agtgtcattt ctaccacctt tacaccgatg attccagcta taacaggtgc
    36121 cgggatggta aaagccattt tggccgtctt gactttgact ggtgtactat cagacagtag
    36181 tcaaacttac atcattttga atactattgc tgatgcagcc ttttatttta tgccagtact
    36241 attagcctat ggggcagcca taaagtttga tgtcaaccca attttggcga ttactgttgc
    36301 tggcattttg cttcatccaa atatttcaca gcttttttct actggcgatg ccgtttcttt
    36361 tctaggaatt ccggtattag ctgcagatta tgcgggatca gttttaccga ttatctttac
    36421 tgtttggatc atgtcctatg tagagccctt tgcggatcga atttcgcctt cgtttattaa
    36481 attctttact aagccgatgc tggtattttt aattatgggg cctttagcgt tggttgtcat
    36541 tggaccgttt ggaacgttgc tgaacgatct tatagcaatg ggggctgaat ggattaacgg
    36601 caaagcagct tggttaattc catttttgat gggtggtttg cagccatttt tggtggttac
    36661 gggtacagct tgggcaatga cgccaatcgc tactatgcaa ttgagcagta cgggttctga
    36721 aatgatcaat gggccaggaa tgctggcttc taatattgct caatcaggtg ctacctttgc
    36781 tgtagccgcg aaaactaaaa acaaagagtt gaagcagcta gctgtgtcca ctgggatcac
    36841 tgctttaatg ggaattactg agccttcact ctatggagta accttgaagc tgaaaaaacc
    36901 attagttgct tccatgattg gtggtgcaat tgctggtttg tatgctggct tgagcggttt
    36961 agtcagatat gcctttgttt cccctggctt agcagcctta ccagccttta ttggagaaaa
    37021 tccaatgaat attgttcacg caattattac gtgtgtaatc gcctttgttg caacctttat
    37081 cattgcttat ctaattggat ttgacgatac ttctgatgaa tcagagcaaa cggcaatagc
    37141 cgaagcagtc attgttgaaa atccggtaaa aggaaccgtg tgtcctattt ctgaagtgaa
    37201 cgatgctgtc ttttctaagg gactattggg aaagggcgta gccattcatc cagaagaaaa
    37261 cagcatttat tcacctgttg agggagaagt cattactatt ttagaatcta aacatgcgat
    37321 tggtctacgt tctaaagaag gtattgaatt attgatccat gtggggatcg acaccgtgaa
    37381 tttaaaaggg gaaccctttg aagtatttgt aaaagcagga gatcaggtgt cagcaggaca
    37441 attattaatc aacgccgatt tcgctgccat tagaaacgct ggctacgatg atacagtgat
    37501 tgtggtagtg accaacagtg aaaattacgc aggaattagt gaagcaagcg gaagcttaaa
    37561 ggaacacgaa ccattgatgc aaattagtta aggagaggat ttttgtgcaa tacaaacagc
    37621 tttcagcatt tccagagaag tttttatggg gtgcctccat tagtgcctac caggcagagg
    37681 gtgcctatca ggaggatggg aaacaaccgt ctattattga caagtatcag catacagaag
    37741 gggtagctaa ttttgaagtt gcctcggatt tttaccatca ctataaagaa gatatacagc
    37801 tgtttaaaga attaggctta aaagcttttc gcttttccat cgcttggacg cgaattatgg
    37861 atattgaaaa taataaaacc aatcaaaagg gaatttcttt ttatcatcaa gtaattgatg
    37921 aatgcctgaa tcagggaatc gaaccgattg tcaccatgta ccatttcgat ttaccttatt
    37981 ttctagagga gcagggcggt tggttaaatc gagcaactat cgattatttt gtggactatg
    38041 tacaggtatt gcttactgag tatggtgaca aggtgaacta ttggctgaca attaatgagc
    38101 aaaacacgat gatccttcat cctggagcga ttggtttgcc accaagtgga gagctgcctt
    38161 caaaaaaaga actctttcaa attaatcacc atgttttatt agctcaagct agagtaattc
    38221 acttgtatca tcagttgaat cttaaaggaa aaatcggtcc agcaatcaat ttaacggcta
    38281 tgtatcaagc tacctccagt ccagaagatg caattgcagc ccataattgg gaaacattga
    38341 gaggctggtc tttcttagat gtagcagttc ggggaaaata caattatttg tttgaaaact
    38401 atttgaatga tcgtggatta tatcctaaaa ttgaggcaga ggatcaggca attttatcct
    38461 ccggtaaacc cgattttatt gcgattaatt attattcaac tgccaccatc gctgccagca
    38521 aaaatgacgg atcagatgtt tccgctagag cgggggatca acagatcatg ttgggagaag
    38581 aaggtgtcta ccgggcagct gaaaatcctt atgtagataa aacgccatat ggctgggtag
    38641 ttgaccctac tggactacga ttaacattga ggaaattgta cgatcgctat gatttaccta
    38701 ttttaattac tgaaaatggc tatggggcac ccgacatagt tgaggaagat agacgaatta
    38761 atgaccagga tcgaattgat tatctagcca aacatattct ggctattcaa gcagctttaa
    38821 cagatggtgt agatgttttt ggttatttac cttggtcagc gattgatgtt gttagtaccc
    38881 atcaaggctt caataagcgt tatggtttta tatatgttga ccgaaccgat gagaatctta
    38941 aagaattaaa acgaataaaa aaagaaagct tcttttggta tcaaaaagta atcgcaagaa
    39001 atggcttagc ttagagagaa cggtgaatct aatgagagta attaaaacgt taaacaacag
    39061 tttaattctt tgcttagata agcaaggcaa agaagtaatt ttgatgggga aagggattgg
    39121 atttcaacta aagaaaggcg atttggttcc ggctgacaaa atccaaaaag tatttaaatt
    39181 agattcactt tctgatgctc taaaaaagga ttacattaaa ctgtttgagg ctgctccgga
    39241 tgatctaatt atttgcgtca agcgaattat cgattatgcc aacgccaagt ttgactttaa
    39301 gctgaataaa agtctatttt ttaccttggt tgatcacttg aagcatgcca ttgaacgcag
    39361 tcaaaaaggc attgtttttc aaaatcgcct gctgattgaa attcaaaagt attacccaaa
    39421 agaatttgag atcggctgct atgctatcca attgatcaac aaaaaaatga atgttcagtt
    39481 gccagaagag gaggctggaa acattgcgtt tcacattgta aatgcccaaa gtgaacaagc
    39541 aaatatggaa gaaaccatgc tctcggtaag agcactaaag gatatcttgg ctatgctagg
    39601 atatctgttt cccgaaaaaa aggttgataa agattcgatg ctttacttaa gatttgtgac
    39661 gcatttacag ttttttattg cccgaatgat taagaaggaa gagttgccgg agaaggatga
    39721 ttttttgctg gcttcagtga agcagcgttt tccgaaagag tatcgggcgg ccttgaaaat
    39781 caaggagtac gttcaagaag agatgggtgc ctcagcaaat caggacgaac tgctttatct
    39841 aacactgcat atttcaaggg tgtactgaat gaagaaaata tcggctgcac aatatataaa
    39901 gacttgttgg ttgtgctaag gctcctgatt ccttggaaaa ttcttctaac agtgcggagg
    39961 tgaatgtttc gacctctacc agtgaagtgg taaagtgttc acaagaaact actagcttgc
    40021 cagaagagct acttggagat tggcagatta ataataagga tgctggtata gagatatcag
    40081 acagtaaagc tgtattccgt ataggaaggt ctcaagtaga gtcaaaggat tattatgtag
    40141 atgtaaacaa caaaatgctt ttacatgtct atttttcttt tgagtatgat agtgtgaaat
    40201 acattgaagt ctctattgaa agaactggtt atagtgaatt tattagtaaa agcgtgggaa
    40261 ttttaactat gcacactaaa gatggacaat atataagttt gggtgtgaag agagtttccg
    40321 gtgagaataa tgagacagaa gcagttacgg agcagacgat ggatgaaaga atcgattcat
    40381 ctaagagtaa tgagacagaa gtagatactg ggaagacgat agatgaaaga attgattcat
    40441 tcatcgaaac atacacagct ataccaccta cttggaagct tgtaaatgaa gataaaggaa
    40501 tcacgataaa tactgataca gagatttcta cagatgctca atctttatca gatttattag
    40561 ctaatctaac aggtatcagt aatgcagtga cagagcaagt tggtaaagac attccacttc
    40621 ggttacttga aaatgggaaa aaagattatt ctgtggtatt taagaacgga caggtaactg
    40681 attacaacga agtatttaaa aaagttattg aataatgatt cttccatctt tttcatttat
    40741 ttaccctacc ccgtt
//
LOCUS       DACDKJ010000019        38786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDKJ010000019 DACDKJ010000000
VERSION     DACDKJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.019"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..669
                     /locus_tag="IV328_001139"
     CDS             46..669
                     /locus_tag="IV328_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937296.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1119..2168
                     /locus_tag="IV328_001140"
     CDS             1119..2168
                     /locus_tag="IV328_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8937297.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2165..3046
                     /locus_tag="IV328_001141"
     CDS             2165..3046
                     /locus_tag="IV328_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937298.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3049..3819
                     /locus_tag="IV328_001142"
     CDS             3049..3819
                     /locus_tag="IV328_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8937299.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4098..4952
                     /gene="aroE"
                     /locus_tag="IV328_001143"
     CDS             4098..4952
                     /gene="aroE"
                     /locus_tag="IV328_001143"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8937300.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5130..6293
                     /locus_tag="IV328_001144"
     CDS             5130..6293
                     /locus_tag="IV328_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8937301.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6410..6820
                     /locus_tag="IV328_001145"
     CDS             6410..6820
                     /locus_tag="IV328_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8937302.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6994..9222
                     /gene="topB"
                     /locus_tag="IV328_001146"
     CDS             6994..9222
                     /gene="topB"
                     /locus_tag="IV328_001146"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8937303.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9360..9677
                     /locus_tag="IV328_001147"
     CDS             9360..9677
                     /locus_tag="IV328_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937304.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9837..10073
                     /locus_tag="IV328_001148"
     CDS             9837..10073
                     /locus_tag="IV328_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8937305.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10070..10468
                     /locus_tag="IV328_001149"
     CDS             10070..10468
                     /locus_tag="IV328_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8937306.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11135..12079)
                     /locus_tag="IV328_001150"
     CDS             complement(11135..12079)
                     /locus_tag="IV328_001150"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8937307.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12375..13319)
                     /locus_tag="IV328_001151"
     CDS             complement(12375..13319)
                     /locus_tag="IV328_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8937308.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13540..14754
                     /locus_tag="IV328_001152"
     CDS             13540..14754
                     /locus_tag="IV328_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8937309.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14776..16791
                     /locus_tag="IV328_001153"
     CDS             14776..16791
                     /locus_tag="IV328_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8937310.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16874..18247)
                     /locus_tag="IV328_001154"
     CDS             complement(16874..18247)
                     /locus_tag="IV328_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8937311.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18273..18557)
                     /locus_tag="IV328_001155"
     CDS             complement(18273..18557)
                     /locus_tag="IV328_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8937312.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18559..20574)
                     /locus_tag="IV328_001156"
     CDS             complement(18559..20574)
                     /locus_tag="IV328_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8937313.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20753..21058)
                     /locus_tag="IV328_001157"
     CDS             complement(20753..21058)
                     /locus_tag="IV328_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8937314.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21147..22178)
                     /locus_tag="IV328_001158"
     CDS             complement(21147..22178)
                     /locus_tag="IV328_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8937315.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22385..23260
                     /locus_tag="IV328_001159"
     CDS             22385..23260
                     /locus_tag="IV328_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8937316.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23412..24254)
                     /locus_tag="IV328_001160"
     CDS             complement(23412..24254)
                     /locus_tag="IV328_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8937317.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24346..26223)
                     /locus_tag="IV328_001161"
     CDS             complement(24346..26223)
                     /locus_tag="IV328_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8937318.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26220..27641)
                     /locus_tag="IV328_001162"
     CDS             complement(26220..27641)
                     /locus_tag="IV328_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8937319.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27644..31033)
                     /locus_tag="IV328_001163"
     CDS             complement(27644..31033)
                     /locus_tag="IV328_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8937320.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31290..32681
                     /locus_tag="IV328_001164"
     CDS             31290..32681
                     /locus_tag="IV328_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8937321.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32754..33401)
                     /locus_tag="IV328_001165"
     CDS             complement(32754..33401)
                     /locus_tag="IV328_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8937322.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33409..34095)
                     /locus_tag="IV328_001166"
     CDS             complement(33409..34095)
                     /locus_tag="IV328_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937323.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34101..35174)
                     /locus_tag="IV328_001167"
     CDS             complement(34101..35174)
                     /locus_tag="IV328_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8937324.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35396..36070
                     /locus_tag="IV328_001168"
     CDS             35396..36070
                     /locus_tag="IV328_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8937325.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36075..37415
                     /locus_tag="IV328_001169"
     CDS             36075..37415
                     /locus_tag="IV328_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8937326.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37564..38550)
                     /locus_tag="IV328_001170"
     CDS             complement(37564..38550)
                     /locus_tag="IV328_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8937327.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11951 a         7059 c         7332 g        12444 t
ORIGIN      
        1 acaaataata tataataaaa aataggagaa aaagtagaaa agaaggtgtt tgttatttgg
       61 gaagtagtta aatcgagagt acaagaaatg aatgaacatt tctcagtgtc attcgcagaa
      121 aatccaagta agtattttcg acgtcttgtt tttctgctca tcttatcatt gctgttaaca
      181 gttgcaatgt ttttattgcc ggaaaaaatc cgagaagagc aagtaaatag tctcgtttat
      241 tcgtccatac caaccagcaa attgaaacct tttgattaca gcaaagctga tcagaaaatc
      301 aaacaaagcc gggcgatatc ggtgatgttc agcaaaccga acgggaaaac ctatcaggac
      361 atgcttgatg tttttaatga tcctgagctt atggaagaat tgaatcgacc gatcttttat
      421 tatccgatcg tttatgatgt acatgaactg gaacaaaaat ataacatacg aacggatgaa
      481 gtgacattta ttttctttga tggaggaaaa gaagcaaatc ggataactgc gggtaaggga
      541 ggaataactg attttgataa ggagttgatc cctgaactga atcgacttcc gttagccaat
      601 atcaaacaat tagaagaaga attggataga acgggagtaa cttcccaaaa ttccgagatt
      661 gctaattgag acattggcgc gatgaaacgg tgggtctata tagcgctatt tttaattatc
      721 agaacaagtg atgtttgatc ttcaaaataa caaataaaaa attaaaaaat atagttgacg
      781 aaattcaaaa attcgattag acttaaatca attcttaagg aaagggtgac ctgtaatgaa
      841 cttatcaaaa cgtatttcag cagaaaacaa aaaaaattgg cgcagctggc gtagataagt
      901 tgtattaatg gtgagacttt cccatagata caacttttta gagtgcattt ctaaagcttg
      961 tatctatgct agacatgaac attgactgcc gcatagatta gaatctatgc ggttttttgt
     1021 gtaaagacaa tgaccgcttt ggccattgtc tttttttgta gactggatac ttgcctgatt
     1081 ataataaata aaaagattga ctttgcagga ggaaaaaaat gaaaaacaaa cgattaataa
     1141 cagttgtagt aattatttta gtttatttag caggtgcatt tatttatgga aaagcctcag
     1201 gcgaaaaaga aagtacttcg acgaaccaga caaaaaaaac aacagttgtt ggggtattgc
     1261 aatacgtgag ccatccggca ttagatgaga tttatcgagg aatcaaagat ggtctgaaag
     1321 aagaaggatt aaaagaagga gaaaatcttg agatcaaatt tcaaaatgga caagccgatc
     1381 agagcaagct agccacaatg agtcaacagc tagtacaatc tgatccagat gccttgattg
     1441 gaatcgcaac acctgccgct caatcattag caaacacgac ttcatcgatc ccagttgtgt
     1501 taggtgctgt aacggatcct gtgggggctg gattagtcgc ttctttagag aaacctggtg
     1561 gtaatgtaac aggtgtttct gatcagccgc ctgttacttc acaaatcaag cttgcttctg
     1621 atttgttgcc aaatgcaaaa aaagtcggaa tcttgtatgc ttcttcagag gataattcaa
     1681 aataccaagt agcacaagca gaagaagctg caaaaaaaga agggttagaa tcagtaaaat
     1741 atgcggtgcc ttccactaat gagattgcac aaacggttca agtgatgagc cgacaagtcg
     1801 attttatcta tgttccatta gataatacca ttgccaatgc gatgcaaaca gtggtaaaag
     1861 aagcaaacaa agcaaatatc cccgtcattc catctgtgga tacaatggtg gaacaaggtg
     1921 gactagcaac aatcgggatc aatcaatatc aattagggct tcaaagcggt aagatggcgg
     1981 cagaacttgc gagtggaaaa gaaaaaccgg aaacgacgcc tgtttataca tttgatcaag
     2041 gggatacagt gatcaatcaa tctcaagctg atcgcttagg aatcattgtt ccccaatcga
     2101 tgaaggaaaa agccaaaatc attacagatg actcgcagca agaaacatct aaggaggatg
     2161 acaaatgatc gtatcagcta tttcacaagg catgctttgg gcaatattag gattagggat
     2221 tttcatgact tatcggatct tagactttcc tgatatgacg acagaagggt ccttcccatt
     2281 aggtgctgct gtctgtgtga cagctattac tcatggggta gcgccgattg tagctacatt
     2341 attaggcgta ggtgctggca tgatagcagg actcgttacg gggttacttt atacaaaagg
     2401 aaagataccg gttattttag cagggatttt agtgatgtca ggattgaact cagttatttt
     2461 gtttgtgatg cgaacaccaa atctttcttt attgaatcat ccgattctac aagacagctt
     2521 ccatcgcttt gggttacctg attactttga cacagtcctt cttggcttca tctctttggg
     2581 catcttgatt gctgtactgc tattcttctt caatacagat ttgggtcaag gctatattgc
     2641 gacaggagac aacgaaacga tggctcggtc gttagggata aaaaccgatc gtatgaaaat
     2701 tttaggttta actttgtcga acggtgtcat tgcattatcc ggtgcgctga ttgcccagaa
     2761 cgacggatat gcagatgtaa ataaagggat tggcgtaatc gtcatcggtc ttgcttccat
     2821 catcatcggt gaagtgatct tccaagaatt gacgctagca gaaagattga ttgctattgt
     2881 tgtcggcagt atcatttatc agctattgat tttagtagta atcaaactag ggtttgacac
     2941 gacttatctg aagttgttct ccgctatcat cttagctatt tgtctgatga tcccgcaatt
     3001 gaagaaagca ctgaacttaa aaacgggttt tgagaaggag gcatagtaat gactgatata
     3061 ttgacaatca aaaatggcag aaaagtcgta aacaatggaa tgaatgaaga gaaagtacta
     3121 ttggatgaaa ttaaccttac aatcagagaa ggggacttca tcactgtcct tggcgggaat
     3181 ggtgctggaa aaagcacact gttcaatacg atcgctggta cgcttcaact cagtcaagga
     3241 tcgatctctt ttaaagatca gctgatcaca aaagattcag aagaaaaaag agcacgattc
     3301 ctttctcgag ttttccaaga ccccaagatg ggaacggctc ctcggatgac agtagcagaa
     3361 aatttgctac ttgcacaaaa aagaggtcaa aaacggaaat tccgtttacg taacttgaaa
     3421 aatcaaaaag aagatttttt cagactatgt caagaagtcg gtaatggcct agagcaacac
     3481 ttggatactc cagcgggtga attatctggt ggtcaacgcc aagcattaag cttgctgatg
     3541 gcgacgatca cagctcctga gttactatta ttagatgaac atactgcagc tttagatcct
     3601 aaaacagcta aagcattaat gcgattaaca aatcagcgga tcaatgaaca aaaattgact
     3661 tgtctcatga tcacgcatcg aatggaagac gccttgaaat acggaaatcg tctgatcgta
     3721 ctccaaaaag ggaaaatcgt tcaagatatt tctgaaaaag aaaagcaaca tttgacaatg
     3781 ggcgaccttt tgcagttctt tgaagaagta gtggattgat ttcgacagaa aaattccttt
     3841 cagtagaagg acttgaaaac tcattgacat cttcctttga ttcatcctat acttaaaaca
     3901 ataaacaatt aaacatattt gggtgttttg taggatcacg tagtcaatga ttttaacaat
     3961 taaatagaat attctttgct ctgacaagta aacgatgaga aaagaatttg ctactttgcc
     4021 acctagacgt ttcgtacttt ttcgtatcaa acgctaagtg gcttttttta ttgaagtaag
     4081 aacaaaggag gaataagatg atttctggac aaaccaaatt agctgggctc tttgctgatc
     4141 cggcttctca cagtttatcc ccattgatgc acaatactgc ttttgaagca aacaacatcg
     4201 atgcggtgta tttagctttc caagtaaatc caatgaattt gaagcaagca gtcgaaagta
     4261 ttcgaacttt tgatatgtta ggtgtaaatc tttcgatgcc gaataaaaca gcggttattc
     4321 cttatctgga tgaggtaagc caggaagcgc aattgatcgg agcagtgaat acgatcgtgc
     4381 acgaaaaggg tcgtttgatt ggctataata ccgatggtgc gggcttcatg caatccgtgc
     4441 aagaagctgg gatttcgatc aaaaagaaaa aaatgactct actgggagct ggaggtgcag
     4501 caaaggcagt cactgttcaa gctgcgttag acggcgctga ggaaatcgta gtttataaac
     4561 gaaacaacca aacattcgct caagtaaaag aaacattcga acaactcagc tatcgaactt
     4621 cttgtaagat aaaagttcgt gactttgccg atacgcatca attaagaaaa gacttgaaag
     4681 aaagctgtct tctagtcaat gcaacagata tcgggatggg aaagaaaatc aatcagtcac
     4741 cgctcttaga tataagcatg atgcatgaga atctagcggt ttttgatttg atttatgcac
     4801 ctagagaaac acgtttatta aaagaagcaa aaaaagtagg agcaaaagga tacaatggac
     4861 ttggcatgtt gatctaccaa ggagcaatcg cgtttgaatt atggacaaag gaaaagatgc
     4921 cgatccatgt gattgaaaag ctgtttgatt aaaaaaggat aatagataaa gatcttttat
     4981 tctcgcataa acggtctcaa gaaaagtaat cctttatgta tcgaagcatt tatttcgtta
     5041 ttttttatta aattatcaaa aaatgatagg cttttgtcga ttggttatcg tatcctttat
     5101 ttatcagtaa gaaacgagga ggaaccacaa tgagtcggat ggatcgctat aaggacatcc
     5161 ataagaaggc gaaacctctg aagcgggata cggggagtat ttttgctaga agagaacgaa
     5221 aacgaacgcg tatggaaaat gaggaagatg ctacccgtat ttatgaaaga gagcagcatt
     5281 catttgaaca tttgaaccaa tacgagcaac agatatacag agatgcacca agagcagaag
     5341 aacaacaaaa aaataaaaaa tttttcaatc ggcaaaagaa gaacaaaccg ttaaaacctc
     5401 gaagaaagcg atcgtggaaa aaaatcattc tatcgatttt gcttgttttg atcctttatt
     5461 caggtatctc attttttatt ggtaaatatg tagctgaaca tgacacaagc ttagaggcag
     5521 cgacttcaga aacctttcat gggcaaaaag cgacgaatgg tgcgcataat attttgatcc
     5581 ttggaagtga tacacgcgga gaagatgccg ggcgagccga tacgatcatg gtcttacagt
     5641 tggatggtcc ttcccataag ccgaaactga tttcttttat gcgtgacagc ttcgttacga
     5701 tcccaggggt aggacaaaat aagatcaatt cagcttatgc atacggagga gccgatctag
     5761 tgcgtcaaac tttagtagaa aattttggaa tcgattgtca atactatgcg aaagtcgatt
     5821 tcaaatcgtt tgaaaaagtg atcgatgcct tattcatgaa cggagtgaag atcgatgcag
     5881 aaaaagatct gaatctggat ggtgtagata ttaaaaaagg ggtccagaag atggatggac
     5941 atgttctttt gcagtacgca cgtttccgaa tggatgaaca aggagacttt ggccgtgtca
     6001 gacgccaaca gcaagtaatg aacgctattt tcagccaatt gaagaatcca ttgaatctga
     6061 tccgtgcacc ttatgcagct ggtaaagctt taggatatac atccacagat gtcactgctt
     6121 ggttcttgac gaaaaacctc ttttcgattg cacgaggagc tggtggcatt gatcgaataa
     6181 gtgtgccagt agacggttcg tggaattatg ggcaaagtga ttatgctggc agtattcttg
     6241 aaattgataa tcaaatgaat caatctgcta ttacagattt tttgaataaa tgatcataaa
     6301 aaagatcaag aagaagctgc gcaaggcttt tgtcatgaat caatcaaacg attcactcgt
     6361 aaaaaacgct ttcttatggt agatttaacc aagagaggag ctgtttacat tggatatcta
     6421 tctgaacttc aaaaatgagt cgcgtgaagc catcgctttt tatgaaaaag tttttcagac
     6481 tgaagcatca ggtatcacca catacggtga aatgcctgga atagaacaaa tgaacttgcc
     6541 agaagaagca aaaaatctaa tcacgaatgc acggctgaac atttatgata cattagttat
     6601 gtttgcagat acgccagaag gaattggtcc tgaattagtt gtgggaaata atgtttcttt
     6661 agtcatccag acaagaaatg aagaagaaac gaagcgccta tttgaaagct tagctgaggg
     6721 tggtaagatt gagatgccac tggatgctgt tccgtgggct cagttgtatg ggatggtaac
     6781 tgatcgattt ggcatcaact ggcagattaa ccaagagtga cagctaaaga tgaatatttt
     6841 ctggaaacat caagcagcaa acggatcatc gtttgtttgc ttgatgtttt tgtgtctatt
     6901 tcgactatac ggtatctttt ttttattgcc agatgcgaaa atatgttcca taatagagaa
     6961 gtccgcaaaa aattaagtaa cggaggtcag aagatgaaga cggtgatact agctgaaaaa
     7021 ccttcacaag caaaagccta tgccgaaacc ttccaacagc aaaagcggca tgaagggtat
     7081 tatgaaatca gcgatccatt gtttgctggt gaagtaacca tcacttatgg ttttggacat
     7141 ttggtagata tggttgctcc aggagcttat gaagagcgtt gggcaaaatg gtctttagaa
     7201 aatttgccta ttttcccaga aaccttccgt tacgaagttc caatggacaa acgtgcacaa
     7261 ttttcgatcg taaaacgaga attgcagtca gccgatacca tcattattgc aacagatggt
     7321 gatcgagaag gtgaagccat cgcgtggtcg attattcgac aagcaaacgc attttctaag
     7381 gaaaagaact atttacgttt atggatcaat tctcttgaaa aagaagcaat ctatgaaggt
     7441 tttaagaatc tccaacctgg aaagaactat tttccaaaat ataaagaagc gcaagcaagg
     7501 cagaacgctg attggctgat tggaatgaat ggaagtccat tatatagctt gctacttcaa
     7561 cgaaaaggga ttgatggcag tttttctctt ggtcgtgtac aaacaccgac actctatatg
     7621 atttatcagc tgcaagaaga aatcaaacac ttcaaaaaag aaacgtactt tgaaggagaa
     7681 ggtttaatta cgacaaacaa agggcagttt tctggaaaaa tcgtaccaaa aaaagcattt
     7741 aaaacccaag aagagttaac gaaggaaata gaaaaacttg gagcgcattt aggtaagcaa
     7801 aacggacgga ttcttgaagt tacgaaaaag gaaaagaggt tgcatagtcc acgtttattt
     7861 tcgctatcta gtttgcagac aaagatgaat cagctaatga aagcaagtgc caaagctaca
     7921 cttgatgctg ctcaaggatt gtacgaaagc aagtttctaa gctatccaag aacggattcc
     7981 ttttacatta cagaaaatga gcatcagtat ctggttaaac atctgcaaaa ttataaaaaa
     8041 tttttaggaa tagaagaagt acagacaact gagatcaaag caaagaaacg atatgtcgat
     8101 gcgaaaaaag ttcaagaaca tcatgcaatt attccgacaa agacgattcc gactcctgca
     8161 cgctttgcga agctttccaa attacagcaa gcaatctatt tgcaagtttt gcgcactact
     8221 gtggctatgt ttgctgcaga ttatatctac gaagagacga ccgtaatgac gggcgtacag
     8281 caattaaagc taaaaagcat cggaaagatc ccattacagc aaggttggca ggaaatctta
     8341 cgcatcaagt caaaaaagaa agaagctcag actcttcctc ctatcgtaga aaatgaagaa
     8401 gcagaagtag aactgaaaag tatggagaaa gaaacacaac ctccaaaacc ctataccgaa
     8461 ggaacgttga tcacagcaat gaaaactgcg ggaaaaacgc tggataatga tgaagcgcaa
     8521 gaaattctca aagaagtaga aggaataggg acagaagcta cacgagccaa tatcattgaa
     8581 aatcttaagc agaagcatta catcgaagtc aataaaaacg aaataactgt tacgccaaaa
     8641 ggaatcactc tttgtaaggc agtagccaat gaaccattac tgacgagcgc agaaatgact
     8701 gcgcgctggg aagggtactt gagaaaaatt ggtaaacaag aggggacacc atctgttttt
     8761 ttagagaata tcaaaaaatt catcctacat ttattggcag aagttcctaa tcaaatcgac
     8821 aaggtagact tttcagatga gattagcggg acacgtcaga taaaagcgat ggagaagaaa
     8881 aacgatcaat taggaagatg tccaaaatgt aaaaagggaa tagtcatgct ttatcctaaa
     8941 atcgctacat gcttgaatcc ggaatgcgac ttcaaattgt ggccaacagt agctaaaaag
     9001 aagctgacta aaacgaatct cagagatttg ctttcaaaag gaaagacaag taaagttgtc
     9061 aaaggtttta ctggtaaaaa agggaaattc gatgcagttt tagtattaaa agaagatatg
     9121 acaatcggtt tttcttttcc atggatcgaa aacagtggaa caaaagcagt tgatggaaac
     9181 aaagaaacat caagaaaaaa taatgaaaac gaagtagagt aaatgaaaac aatgaaatag
     9241 cggaatattt attccgaaaa gtgagaatat aaataccaat aaaactaaaa aatatcagtg
     9301 aatcaagcaa aaacgttggg gtaggctgat tttatataga tagcaaggag tttttttatt
     9361 tgaaaaaagt gcattcattt ttaaattatc ttatgggagc aatcacgatt gtggctgtgt
     9421 accaagcatt tctaaagtat cctgattacg ggacgaagtt gttgtcattg acgcacaatc
     9481 aaacagcata ttcaccaacg ttgattttat cgactttgac tgcaggagct aaaagcgcta
     9541 cttatttttt tcataaaacg acaagcatgc taaagcgaaa gaaacagcga aaacaattgt
     9601 tggtggtggt agcggctatg atcattattt attttctacc agttattaca caagtatttc
     9661 atcaatattt tggttaagaa tacgagagtt tgtgacaaaa gtcatgtcac aaactttttt
     9721 ctttgtcatt ttttcgtaag aaaaagcaaa ataaaagtat atacattgat taaaagtata
     9781 tacttggata taatgtactt actatagaat gagaatggta tagagggtgg cgaaatatga
     9841 aaaaaatgaa aaagtataaa attagaaaat ctggcaatag cgatgtaaca actatcccac
     9901 cagaagtaaa agaattcatg ggtgtgcaga caggtgacgc aatcagttat gtttttcaat
     9961 ctgatggttc tgtacgaatg atcaaagcac aagaagaacc tgatattgat tcattagttg
    10021 attcgatcat gaatcaatat gaagacgcgt taaaagattt ggttgattta tgaggtatct
    10081 ttcgactaaa gaaattatca aaatcaacgc gaaagttatt ttacgtcact cgccaggcga
    10141 aatgatcggt gtaaaagatg caaatgcatt agatatggcc gtcaaacagc cttcacaagc
    10201 agtattcaat caagaactct atcctgaaat ctatgaaaaa gcggcgattt tagcaattaa
    10261 tttagctaaa aaacatcctt ttcataatgg caataaacga actgctcttg ttgccatgtt
    10321 gttgtttctt caattgaata attgtccagt tgctttttct cgtcaagaag ctgttgattt
    10381 tatcataatg attaccacta gttcattgga tttcgattca ctaaagtcca agataaccag
    10441 ttatttacga caaaccgcaa taaaatgaac gaattaaaaa ctttatttct caattagatc
    10501 agcatgtcat taaggaatat gctgattttt ttatgtttaa tcatacaaaa tagttttttc
    10561 ataaaaatat ggaaaatatg gattaatttt gaaataattg atttaaaagg aatacaaaag
    10621 agtgaatagt aggaaatagt ggtttagaac tagtgggctg attttttata tattttagtt
    10681 tacataatat atattataca aagtagattt aaaaaatgat aaacctctct cgaacggttt
    10741 tttatcaaaa atcgtcattt ttccatcaaa atctgcttat gcaaaattat aaaaaaattt
    10801 tgcagttttt cttacttttt ttgtcgttta attgaatgat aaaaaccagt ttctttattc
    10861 agttaatcaa atatgaacga attagatatg aatcattgta tttgccgttg cggaaggatt
    10921 ttttaattaa ccacagataa acattgataa cgattcgctt attgtaatca acagattaaa
    10981 tttcatatgt tcatatgtac ggatacattt tcggaccgat taattttaac cattctgttt
    11041 atttgcttac ctattagata tcaataaaaa actgcttaac agctatttca gctgttaagc
    11101 agttttagga tttttagttc atattcattg tttcttataa tacagtgttc aatacttctc
    11161 ttaaagcact tgttgatttg ctgaacattg ctttttcttc ttcgttgatt gataattcaa
    11221 caacttcacg aacaccattt tcgtcaacga ttgatggcac acctgtaaag atatcttttt
    11281 cgtcgtattc accagttaaa taagcagata caggcaatac agcttgttcg ttattcaaga
    11341 tagcttttac gatacgtgca gtactcatac caataccata gtaagttgct tttttacgat
    11401 caatgatctc ataagcagca ttacggactt tatcagcaat cacatctaat tcatcttggg
    11461 caatacgatg gtctttttct acaatttcaa acactggttt accaccgaca gttgtatgtg
    11521 accatgcagc aacttctgaa tcaccatgtt cacctaagat atagccgtgg acgctgcgtg
    11581 ggtcaacttt taatttcaat gcgatttctt tacggaaacg agttgtatcc aatgttgttc
    11641 cagtaccgat aacacgtgat gttggcagac cagattcgtt ccaagcgata tatgtcaaaa
    11701 tatctactgg gtttgaagca acaacgataa taccgtcgaa tcctgatccc atgatttctt
    11761 ttacgatttg gcgcatgatt gacgcatttg ttttaactaa atctaaacga gattgaccag
    11821 gtttttggtt gataccagct gtgataacaa cgatatcagc atctttacat tcttcatagc
    11881 cacctgacca tacagccaca ttttcatcgc cccaagccat accatctaaa aggtccaatg
    11941 cttccccttc tgctttttct tgattcacat cgatcaatac taattcatta gagattcctt
    12001 ggttgatcat tgcataagcg atacttgttc caacaaaacc tgtaccgacg attactactt
    12061 tacgacttgt ttttttcatg aataaacagc tccttttcat aactatttgt tactttcatg
    12121 aaaagtatag tcttgtttat tcattttatc aacatgttaa ttataatagt tgttattata
    12181 atttttgatg gaaacgaatt cctgaatacg cataacagat aaatgacaaa tgtttacacc
    12241 tatatcccct gataatacaa ccaaaagttt agaactgaaa ataaatccaa aaaaattcgc
    12301 tgcaacgata taataagaca gcaatcgcac ctaaaacaat tttccttcat atgctttagt
    12361 gaaagatttt cttcttaatg tttatttctc cattcattgg gagtacatgt tgaatacttc
    12421 ttgaataatt tgaataaaga agtcgtttgt ttatatccga tacgattagc gatttcttcc
    12481 aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt cgaatttttt ctttagaacc
    12541 agttcttgaa aggaagcatc aaaatacatt ttcaatttat tactaagata gaccggattg
    12601 tagccaaaat gagctgctgt ttcttttaac gtacaagaag taaaatgctt atcgatataa
    12661 tctaatactc taagcatctc attttctgcg catccatctg ctgatgaaaa gctttgttgt
    12721 tctaataaat gagtcaattc gatcagtaat agtgatgtat agagatttaa cgaacggatc
    12781 ttatgactgt atgtatcaaa ataaatcaac agcatatttt caatcaaatg cgtcaaacga
    12841 cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt cccctttttc ttttgatgca
    12901 tttatcaaaa aagaagaaag tagattattt gtgcttgcca aatgattcaa tatctctgta
    12961 gataaggttt cttcttttaa taagatgttg attaaaatat cctcttgccc tgtgtattcg
    13021 attctttgag tagcttcttt atcaatgatc accacgtcac tttcttccat ctctatcctt
    13081 tctccattaa taatttgtaa acatgtaccg ctatacatat aattgatttc aacaaaatca
    13141 tgtgtatgct cgggaacttc cgcatatcga cggtgtttac tgatatgaat atttccctgt
    13201 tgaaatagat cccctcggtg gatttttggc ggtgtctgaa gatggtaagc taagtttaaa
    13261 tcattgatat tcattccttg ttcacgctgc agagtttcga ttgattcttc gttacgcaaa
    13321 aaaaatgtct aattcagcta gttccaattg actccctcct ttttgtaagc ggtttctttc
    13381 gcttattagt tcacgctgta ctgctattat atgcaaagct atttttggtt tcataaagac
    13441 tttctatggt tgggacatga acttcctgtg attccgctat tgagagaata accacttcat
    13501 actatatttt tgataagcgc ttacaaaaat gaaaggaaca tgcttatgaa taaaaagatg
    13561 atgaaatttt ctctgctgtc agtctctctt attttgactt ctgctggtgc agtcacgtct
    13621 gcaattccca gtatgtcagc tacttatgcc acgcgttctt tatctgctgt tgaattatta
    13681 acgactgctc cctcttttat ggtcactatt tttgttttga ttagtagttt tattgctagg
    13741 aaaatcggac aaaaacaaac tgtgatcatc ggattagtga ttgcatcgat ttgtggtatc
    13801 ctccccctct tctcacaaag ttacagtatt gtcttagctt caagagctgg actaggtatt
    13861 ggctttggat tgatcaattc tttggcagtc agcatgattg ctgcctttta tcacggagat
    13921 gaacgagcct ctatgattgg ttttcaaagt gcatttcaag gatttggtgc agctttaatg
    13981 acatttgttg caggacagct agtacagttc ggttggcaat ataccttttt agtctacgcc
    14041 atcagcttac ctattcttgt cttgtttgtt ttgtttgttc ctacacctga aacaactgaa
    14101 cttcaatcgc atggcacagc cccaacaaga caagtaatca aaacgaatcc taaaatgatt
    14161 agttatggta tttttctctt gctggtcatc gtggtatata atgctgtatc gattaaatta
    14221 gccacagtcc tgacgactta ttctctagga aacgaaacta atgcagcgaa tatcttaaca
    14281 atcatgcaat tggcaagtat ggctgccgga gtttgttttg gctggcttca gacaaaagca
    14341 catcgctata tgcttacact ttctttacta ctgatgggaa tcggctttgt attgattgcc
    14401 actgtgataa acctgtatgt gattgggttt ggtgcgatcc taacaggaat ttctttttcg
    14461 ttattcattc cttacttatt caaccaagta agtatccaag cgcccaagta cgcggagagt
    14521 ttcaatactt caatcttatt ggttggcgct aatttaggta ctttcattgc accatatggt
    14581 ttgctctttc tctcttttgc ttctcttggg agtaaaacac ttgctgtttt cgtcaatggc
    14641 agtatcttgc tgttgattag cgcgcttatt agccttctag tagtacacag gacgaataga
    14701 aagacagtga taagagtaga tgaagaaata aaagaacaat tgacaacaaa ataaatttga
    14761 tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata gatggctttg gtatcctgga
    14821 gactttgaga tccgccatgg actgctacaa aatttccaac gggaagaacg aggttttgac
    14881 tggccagcgt actggtacat ggatgattgc catcggaacg tcaagtttaa gagatattac
    14941 tttcttgatc aaccttccat gtttaaagtg acgatccaag gagttgggta tgtagaaatt
    15001 aatggacaaa aacatccatg tggaaaatgg ctgacttgtc cagctggtaa agcaaaaatt
    15061 cgtatctttg taggacatac atcagggcta ccagcgatgt ttatcgaggg agacgaagta
    15121 aaatcagata ttggctggac agcaagtaat tttattgaag aatatccagc tggctggagt
    15181 ccgctttatg tcgatatcgc aaaagatcca aatcaaatct attatcaaaa agaatgcgtc
    15241 catccagtca ctgaaacgga gattggcgga ggaatcttat tcgattttgg gcgagcagtg
    15301 aatggctgtg tagaactaat atctctggat gaccatatcg aatctatcac tctatgttat
    15361 ggagagtccg atgtagaagc tatggatatc gattattgct actataagca aataggaatg
    15421 acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt tgtttatacc agaggtttct
    15481 actggaatgg ttcaacttca agtttttcat gaatttttat ctctttcgaa acaagctgct
    15541 ttttcaagcg attcttcttt actgaatcag atctggtcag tatcagaaga agccttcaag
    15601 ttatgtagtg atttgttttt tatcgacggt atcaaacgag atcgctggat ttggtctgga
    15661 gatgcgtatc aatcattcat gatcaatcaa tatctttttt ttgacgagga aatcaataaa
    15721 cgaacgatat tagcattaag aggacaaaat gaaatcaagc agcatatgaa tactatcgtt
    15781 gactattcga tcttgtggtt gattggtatc gaaaaccact atatgatgac gaaagactat
    15841 gaatttttac gcatcgtcta ccctaagatg caatcattaa tgagatactt gatggagcaa
    15901 acgaatgaat tagggtttat ttatgggaga gcaaaagatt ggatatttat tgattggtct
    15961 gaaatggata aagaaggaac gcttgctgca gaacaagtct tattactgaa agcctatcat
    16021 tccatgatca cttgcggaga tatcttagct gaaacatatg gagagaacga aaattatcat
    16081 actcatctct atcaagaaaa atatcaacta ttgaaacaaa acctatttga ctacttttgg
    16141 gatgaagaac ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa tgtgacgcgt
    16201 catgcgaata ttttcgctgt tttattcgat tttgctaaca aagaccaaca gcaatcgatc
    16261 ttaaagaatg tcttattgaa tgacactatc acacagatta ctactccata tttcaaattc
    16321 tatgaacaag atgctttatg caaactcgga cagacgaata tcgtctacca aacgattctt
    16381 gattattggg gagggatgtt atctcatgat gcggtaactt tctgggaaga atacgaccct
    16441 actcaaacag gaaatgaaaa gtacagtatg tatggagatc cttatggaaa aagcttatgc
    16501 catgcatggg gagcaagccc aatctatctt ttaggacgct attttatcgg tttgtatcca
    16561 acaaagccag gttatgaaga atttatgata caacctaatc tagattcatt tgaaacactg
    16621 aattgtacgc tacctatcaa agatggaaag atttcgcttg aaaaaacaaa agataagatc
    16681 gtgattactt ctgatagaac aggcggaaca gtgaaaatga atggtaaaag ttatcctctt
    16741 ctagcaaaac agccacttgt cttaacaagc tattcaaaaa taaatgcata aaacaaaaaa
    16801 ctgcttgcga catatcagaa gactttaaat cttcttctga tattgtccaa gcaattttta
    16861 ttttgaagtt tgcttaattc tgttctttag aaacaatgtt tgttcttttt ccggcaaaaa
    16921 gaaagctaga taatacgaca agtaaaaaga aagcgatgat tccaactgtc aaggctattt
    16981 gaccgtattc tcctagtttc ccaagtacaa gtcctggtac tagataatca gtatcggcaa
    17041 atgtagaaga agcgatttta agatctccta atactggaag gataaataat ggcagccaac
    17101 taatcaataa gctattgaaa gcagaaccta ataccgcccc tcttctaccg ccagtcgagt
    17161 tgccgaagac accagaagca gctccgcaga agaaatgtcc tactacccca ggaatgataa
    17221 ctgtagtttg caagcctatc atagcaaaca tggatagtgt tcctacaaag aagctaacaa
    17281 aaaagccaat taatacagca ttaggtgcat atggaaaaac gataggaaca tctaatgctg
    17341 gtttagaatc gggtacgagt tttttagcaa tcccttggaa tgctggaacg atttctgcca
    17401 acaccattcg aaccccttga aggacgataa caaacccagc tgtgaatgtt ccagcttgga
    17461 tcagtgcaaa gattacagcg ttgtctccat tgcttagttc tctttcaaca aatccaggac
    17521 cagctaacaa agcaagtgcg acatatacga tcgacatcaa gagcatgatc gatacagtag
    17581 aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa cggtctttgt
    17641 ttccaaaaaa ctttccgatc catccactag aagcataacc gatatttccg gtatgcccta
    17701 acgcaacttg attgtttcca gtgatgttct tcataaacgg ttgacaaaga gcaggcgtaa
    17761 tagttaatag tgttccttcg aagaccccgc ctaataagat catccacaaa tttgcagtaa
    17821 accccgcgct tactaaaatg actgctgtta gacaagaaac ataaagcatt gcttgacctg
    17881 taagaaaaat gaatttatat cttgtgaatc tagcaagtaa aatattcacg atcatcccta
    17941 ctagcatgat caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc actactgctt
    18001 cattgctagg aactactcct tgagtatgca gagctgtttg gaacatcgaa gcaaaagggg
    18061 ttagtgaatt actgattaaa ccagcaccgc cgatcagtac taggaatccg acaaatgttt
    18121 tgatagttcc ttggatcact tctgaaaatt tcttcttctg aagaacgaga ccaagcagtg
    18181 caatcaaaga aacgaggatg gagggctgac tgacgattga cacaaacaaa ttcaacaatt
    18241 tatccattga tagttcctcc cttgaatcat ttttattctt ttgacaagat cttcgttact
    18301 ttttctttta cttcttcgct gtcaatgatc gattgcaaag gtacgagctt ttcatcaggt
    18361 atagaagtta gcgtatcttt caaagaatgc tcaatgaaga aataatcagc catatcgcta
    18421 gatacacttc caacatccgt atgacttact tcaaactcgt ccacatctgc tccaagatct
    18481 gcaagtgctt gtttgatgtt catctctacc ataaaacttg ttcctaatcc actttgacat
    18541 actgcaaaaa ttttcatatc attcgtctcc ttttcttatg atcgctaata tttcttcttt
    18601 tgttgatgcg tgtaataaat cttgaagatt ttgtttatta gaaagtagct ttgttaaatt
    18661 tgccaaagcc cgaagatgtg cctcattatc gatagcagct aaacagatga acaaactaat
    18721 gggatgggct ggattatcaa gcagatccac aggttgttga agtttcagca aagacatccc
    18781 tacttcgttt acgccatctt ctggacgagc atgaggtaat gcgatgtgat cgccaatatg
    18841 gataaacgca ccatactgct ccacgcgctc gatcatagct ttgatataac tttcttctat
    18901 tttattttct gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc agttttttgg
    18961 ttgatccgat aattggatca tttcttctgt taataattca gatagcatag gacgttgatc
    19021 ctccttcttt tctaaaagtt tgttcatttt acgatttaat acttgataga gcttttcttc
    19081 gtctacgcct tcttttaatt cgatataagg cagtagcgcc gacaacaatt cttgggcaga
    19141 aggcattgaa aatcctggta tcagccaatc tctttgtact tgattgatca gctgattttt
    19201 ttccaagtca gtcatgattg gatgcagaac atagagagac tttttcttat cagtaagtgt
    19261 gagaggtaca gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt gtaagcggtc
    19321 agctgaatta gtctctgtaa aaaagatcat cgggaataat tgctgaagtt cactttttaa
    19381 gatcaatgaa gagctaatgc cacttggaca gacgatcacg gctcttattt ttctatttct
    19441 ttcttctgcg tccgcttttc tgatctctcc gccaaatagt atagtgaaaa aaccaatctc
    19501 ttcttcaggg atgctcttcc ctattcgtct ttctaacgga agtaatgctt ttcttgtcat
    19561 ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc atcacattag gtaaataaaa
    19621 agaataggaa atacgaaaat aggcagggac caagtgataa aagacattca tcaataattc
    19681 acgaaacgaa gcaaattcta cggccatcag gaattccatg cgatggatga cttctgaaga
    19741 acattccagc aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga tcgatgctaa
    19801 gcaaagagag aaaaatataa tctccgaatt ttgatgttcg gaatagccaa ggccagtcaa
    19861 tacttgtcga ctaatgtcag tgactgcaag agaagccaac agttcagcat cttttaccag
    19921 ttccatggat gtttttccaa ttcgaatggt tgtcaaactc aaataataag ctaattcatc
    19981 cattcgattt ggtgcgaatc gaagatctga tagtgaaatc gttttatcta gcaaatctct
    20041 aactttggcg taataatcaa tggaaagggt tgcggttaaa tacgtgagcc caaatcttcc
    20101 agcaagtgac tgcatcaaac gagcagtgcg attttttgct tctcttctta atattgcttc
    20161 tgaccctagc agcctaaaac cagttttcct agtatacgac agctgacaat cttccacttc
    20221 taattcattt cttatctttt tgatatctga caggactgtc cctttagaaa cctgaagaaa
    20281 atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca aataaagaag
    20341 atctcttcgc tcttgttcgg tatacacagc ttcaaatcga gtaatatcca aaaatttttc
    20401 tgcccagtcg attgctcgga atttctttat acggatacgg gtatcatcca catattcggc
    20461 aaaaggagtt aattgtttga tcgcctcgct tgtatttact tcgatgatct ctgctgcttt
    20521 ttctagactg atttctcttg gtgttttcag gagataacta attttgtagt tcacagcgct
    20581 gttcacctcc tacttatatt ctagtatggt agcgctttta aatcgagggg tgtttggtac
    20641 aacttacagt tggatttttt ttacgactgt accgaacgat ttctatcgaa tggatacaga
    20701 aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc gatcaatgat
    20761 gatcatgttt attttttgag gtcagttctt caaatttttc tttgacatct tcaaccgctt
    20821 tttcgatatc tttttcaaag tgattgggct cattaggctt tgtttcctct tctacagaat
    20881 ttgaaccaga taaagctgtt tctttatttt ctgtttcacc agagtttgtg ccagctaatg
    20941 attcttcttt gtcttctgtt tcaccagaat tagttcctgc taaagccact tcttcttctg
    21001 ttttaggggc cattgaacct cttaatcgtt tgtgtttttc accgtcataa tttgtcatgt
    21061 tggttcctcc tagtagttct atactttttc atcgtacaat aagacagttt atgcgtcaaa
    21121 aaacaaaccc gattcttaca aatatgtcat atttttcctt ctgattcttg aatgcttttg
    21181 attccttctt ccaaaatttt catttgtaag attgtttctt catctttgat gactttttct
    21241 gcgccattaa ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc accaacctcg
    21301 gaaattactt tttcttcatg ataactccct tgttcatcta tataggtcaa tacaccataa
    21361 tgttcgggca aatcgatacc aaagtcttca tgatcaggca tgtaaaacat tttcagatgt
    21421 tcttcttgtc gatcttttgt ttgtttcacg aacatccctt tttttccgta aacgacgaaa
    21481 cttggacgtt ctttgatgcg gaaataactg gattttatcg aaattttcat atttccatag
    21541 aaaaaatcca gatcgaagta atcattcatt cgtcctttcc ctaaaagttg acgtacatca
    21601 tagctgatat catctggttc gccgaaataa gaaagaactt gatctactgt atgacaagca
    21661 tgtccatata aatagctgtt cgcagtagaa aattcatgcg tattttctgg tacttccggt
    21721 ctgaaataat cataatgcat ttctacttct aaaatatccc ctaattttcc gctttcgatt
    21781 actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca ttggataaat
    21841 agaccttttt cttttgctaa tgcaaataac tcttctgctt cctttgacgt ttctgcaaaa
    21901 ggtttctcta ctagtacatt cttaccatgt tcgagtgcca tttttccata ttcataatga
    21961 aattgactag gtgtagtgat gacaactaat tggatctcag gatcatccca aagatcagac
    22021 agctgatcag tatattcgat gtcttcgaat gttttccaca ctggttcaga tggggaaaaa
    22081 atttttttga tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg atagcgattt
    22141 gtactctttc cattgccaag ataagcgatt gtaagcatga gtggtgcctc cttttattga
    22201 tgttttcagt atatcctaga aaagcgcaga aagtatttat aatagctatt ctggtaaaaa
    22261 agttcagaaa aacacttgtt atcattcggt ttggtgaaat taatgttcaa agtaaggata
    22321 aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt aagatagaat ttggaggtca
    22381 gatcatgctt attgaagaaa aactaacaaa acaagagttg tttaccacga cagagaaacg
    22441 gattgtcgat tacatccgta ggaatatcga agctgcagtc tacatgacga ttgaagaatt
    22501 agcaaaagct acgtatactt cccattcagc tatcattcgt ttatgcaaaa aaatgggatt
    22561 cagcggattt aaagaattcc gtttcgagct ggggcgggaa gttcatcaat tgattgctca
    22621 gttcaatcaa acgattgatc ctaattttcc tttttcaagt gaagatagca ctcagcaaat
    22681 tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa aaagcacaat tgcagattga
    22741 acatgaagat ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa tctttctttt
    22801 tgcaaaaggt gattctcaaa tcacggcaag gaaattccag aataaaatgg tcaagttgaa
    22861 taagtttttg atcatggctg aagaatacag tgattcttct tggaatgcag ccaatttgac
    22921 tagcgaggat tgcgccattt ttattagcta cagcggtcgc atccatcatt atgaacgaat
    22981 aatgacctat ttgaagcatg tagaggcccc taccttgctg attaccggaa atcagcattc
    23041 agaaatggcc aaacaggctg atatgtgtct tgttatatcg caggaggaat atgattttgc
    23101 taaagtagcg actttctcct ctcaaattgc cttcgattac gtattaaata cgttattctc
    23161 agtgatatat tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca aacaagaatt
    23221 gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa gaaaagatcc tcatttagta
    23281 ataagaaaga tagtgtttga taggctctct ttgatatcat cataaaaaca ggctgaagtt
    23341 ttctttttca aacctcagcc tgtttgttta cggaacgatt cgaatagata tattatagaa
    23401 atgaggaata gctaacttct gtttcttatt ttgaacgatt gacgaaaaag atatataaga
    23461 aggaggaaaa tcagtataca tgcagctaac aatccataca ggaaaaatcg tttgttctcg
    23521 gatactttct gtacttcttt ttgaacttct tttgcatgat aaggaacccg gtgccctctg
    23581 acaagtaagc gatgactgtt gaccatataa ggtgtacaag ttaggagtgt cacgaaatcc
    23641 tgcccttttt cgattctcag atcttttgta tcagtcggtt ctaccgtctt gatctgatcg
    23701 acttgataag cgagtgtccg tccattgatt tcgatataga aagaatcacc taaagtcagt
    23761 ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga aataacggca
    23821 tgtgtatttt ctccccccgt aggataagac gttccttcca ataatgaggc acctttttct
    23881 agcagcacag tattcgtttc atcaaaaatt ggcaaatgga cattgatctc agggatcgtc
    23941 aaaataccga tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg ttcttttttc
    24001 ttagtaaagg gatctgcacc tggatttcca ccttcttttg ctaattgctg gtttttcttt
    24061 gtcatgtttt cttggatttt agccatgact tcttcatttt ctttgaccgc ttgttgctga
    24121 taatgagaga tgatctgctg atccagataa ttattcagcg cgtcgctaac aaatggataa
    24181 gctagtatcc ctattcctga gagaagcaag aaaagcatca gcagatctag aagtttcctt
    24241 ttctttggtt tcatttgtgt tctccttgtg atgactgttt gataataaaa gaaaaaggag
    24301 catttgcgta atccgctgct cctttttcca attcttgcaa aaagactaaa tctgactgtg
    24361 cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg actactcctg caccgatata
    24421 cacatagatt cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat ttggtatttt
    24481 ttcaggagaa atcaactgtc ctgctgttac ataagattgt tcatcgatag taaatgctac
    24541 acggtttgtt aatggaacat atttttctgg cgctttcgtt tcttccagat agtacgtgcc
    24601 atattttaga cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa ccgttgctga
    24661 ttctttcgcc gatacccagc tgacggcttt tgtggatggg tcgatcgata aatatttcgc
    24721 tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt ggtctgatgt
    24781 aacgtcacca tctactttaa caaatcgttt gcccccagta acgacatcga ctgacggtgg
    24841 tgtttgatca tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc ctttggttgg
    24901 atctgctttt tcgttcaaat gcatatagta aacgaatttc aatgtaccgc caggagttaa
    24961 tgaaggaata taattcggat caactgaaac cgtgaatccg tctgtttgct cagtgacaga
    25021 ataatttact gggtcgattt ctttatttcc atcatataag gcataagcat acgttcctga
    25081 agaatcatta tcaaatgtta aagcagcgtc atgagtatcg ataagtttga atgttgtgta
    25141 cttgttttgc gttccttctt tatcagcgat acctaatggg atattgacag aaatctcata
    25201 ttgaattttt tcaccgattg cgacatcttt tccattcaat tgaggtgttg ttttatcaac
    25261 tttagacgta tcatttttga tggtcttttc tactggtgtt tggctatttg ccaggatctc
    25321 aaaaggcgtg attgtttgct tttcaatcat ttccgcatta tcaggagctt ttacttcttc
    25381 taaattatat tttccaactt ctaaatgatt aacgatcaac tggccttttt caatagatgc
    25441 ctcggcaaag tcattgttgc cgatgtcata agaatgacca gtaatgaaat gtttggcttt
    25501 ggtttgatca gttgtccaag tgtacaatcc atctgtgaca ctttggatgt attttttgac
    25561 gcttggtgtt ccttcttctt tagaaataat aaattctgct ccatttagtg cttcgttttc
    25621 ggcagtaccg atttttgtaa ctttcaacgt tccatcatta ccgactgtat ttttagggta
    25681 gagatggata gtatctagtt cttctgtccc gtatttataa gagccatctg cttgtttgat
    25741 catctcatat acagggaaag ctaaaaccat gtttgcggca gctgacactc cgtcttttgg
    25801 ttcttctttg atcgtgtaga ctgcatcttt cccattttgt tttttaggta aagataaagt
    25861 gacatttcca tcagcatctg tcgttcctga agcaactggt gtaccaggag tcaaagactg
    25921 gactgcttgt tttgctgcat ccacggacgc tcctttatct cgttgcgcat aaaattcttg
    25981 agtgacgtta taaactgaaa atgaaatatc ggctaatcct tggtattgat cgaattcgct
    26041 catttctttc ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt tctttttatg
    26101 aaggatgact tgagctgctg tctcctctgc aaacactttt tttgcgccga agctgtttgt
    26161 gagtaatggt aaaataagga aaaggactcc taacataacg tttatttttt tatgattttt
    26221 cattttttca ctcctgttct ttttttgata aacaaccaga ttgcaaagct tgcgaatacc
    26281 ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg tttttggaag aaatggacgg
    26341 ctattttctt ttttattgac tactttcaat atactgtccg cctctttatc ctcagaaaga
    26401 aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa tataaccaga aggtgcttcg
    26461 atctctttta atcgataact tcctgttttc aacccggaaa ttgaaaaagc gccattttta
    26521 tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc gttggctgtt
    26581 tgctggaggt aatttcctgc tttgttttta ataaggaata ctgcgccagc taatcgttta
    26641 tctggtttat ttgtctcgat tttgacaaat tgcttactcc ctgtatatac agagactttc
    26701 tttgtactga caccaaaacc aaaatccagt gttccgttgt tgttgatttc cttgttcgcc
    26761 tgtgctgtac tgcttagctg catctgataa gaaatggtca gtttttttcc agcatatttt
    26821 tccaacgaag ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg tatgacaaag
    26881 ccttctgtca gcttttctcc attcgatgaa atcgttaaac tattaggtaa aaacgttaat
    26941 gcttcatccg cactatctga caattcgaac ttttgatagt caaggatatt tacaggaatt
    27001 gtcgtatgca aggaataagt gattttttca ccaatcgtaa aatcatttct aggctcttct
    27061 aataccttct caaaaggtgg atcagggtag tcgttttctt catttttagg atacaaatgg
    27121 atagttgaaa gtttttgatt gttttgtcca tgtacaggaa gaacaactac catattctct
    27181 gccttttctt tgacgacttc tggtactttg gattcaatga aaagatacgc tttatctctt
    27241 ttttcttgat ctttagcggc cagagaaaaa gaagcgatac catcttcgtt gttaagagtt
    27301 gtagttgttg cttctgcggt aaacataccg gaagacgcac cgttttttgc gatctctgct
    27361 tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc tgtgacatca
    27421 taaacttgga atgtgacacc atttaatcct cgatacgttt gtaataaagc tttttcttgt
    27481 ccgtcatttg gatgattttt cggcagttgc ccgtttggga aaagcaattt gtgtaaagta
    27541 atctgaacca tttctgtttc tgtttctgtt gctacctgag taaaagctgg tttaaatagt
    27601 aacaagaaga gacacatact aagccaacca agttttttca tatttagctg ccccctcttt
    27661 cttttttcaa aaaaagatac caaatagaaa acgcacaacc aatcatccct actagataga
    27721 ttcctaaacg gccaattcct cccgtttcag gtaatggtac ttttgcctga ttcgtgatgt
    27781 ctaaagaaat ctggttgttt ttggctcctg gtgacagaac agattcatga tcttgtccat
    27841 ccacttgaat tgacccatct tcgtgtataa ctatagtaac tggctctttt agaccttggt
    27901 atccttctgg tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa ttttcgaata
    27961 gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa gtccatttct ggtccctgca
    28021 gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt accgttagat
    28081 caaacgcttt tagtcgattt tgatgagtga gcgtccatac attatttgtc atctcggtat
    28141 tttttcccgt gtaaatgatt tttccatatt tatcgatttg gaattcataa tttcccggcg
    28201 aagtatcata tccggcagga ccagctgttt caacgactcg atatttacca ggtgtgagtg
    28261 aatcaaaact ggcaagacct ttttcattcg ttgtttggct gtcaattggc tgataagtac
    28321 catttgcttc ttttttctgt agggaaaatg ctgctccttt aagatttatc tctttgtccg
    28381 tcccttgcat cgcatactta cgtactgcta caggaacttc aacaaaagga ttttcaatag
    28441 tcaactggat cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt accgtaccat
    28501 cagaagcgat cttgatttcc caagtagtct ttttgcttaa tccatgccct tctggagctt
    28561 ttgtttctgt cagcgtatag gtcatttctt tttgcaattt gacattttct ggaagagaat
    28621 aggttccgtc gccatgatct gttagtaaag tatcaataga atctcctgtt aatttgaaaa
    28681 cagcgccaat aagatccttt tcacctgtag aggaattttt cgttattttc aagtttaacg
    28741 gtgtgaattg tttagtattt ttccatgtca ttgcatctat ttgttgagaa tcgtatcctg
    28801 gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg accttgatta ttgtattgag
    28861 gtagtgataa tatctcttga taactttctc cgctatttgc agataatttg tcaatgtctg
    28921 cacgttccca cgtatttgtt gtatctttag ctggtttaat gattcgaata tatccgtttt
    28981 tccaagctgc agcatttgtc gtatgttccc gttgaatctc aaaagtaact tgatctggac
    29041 gatcagctag attgttgtca aattcttccc ataacttttt gatgtgaaga ctgactccgg
    29101 gagctttagc agatggtata ccgaattcag ctaattcatt ggtatcaatg tttggctgga
    29161 aagttgtcct gccgttcatt tgataccaga aatttggatg gaagtcctca ttttctgttt
    29221 ggattctcac ttggtaatgg atttggattt cttggtcttt tcctaaatag atctgattgc
    29281 tcttgatggt atttccttct atggaaatag atggagtgac cgttgtagga cttgtcccca
    29341 cactcttgac tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt ggatcattta
    29401 tttgtccatt gcttacagtt gctgagatct gtacagcttt tttggctagg tattcagaga
    29461 tatctgttgc atgatcagct gattcatagt aaagatcgcc tttttcatct gatgaaacca
    29521 tttgtcgcat acgagactct acttctgctt ttgagagacc agctgccgga tcgctttgaa
    29581 gttggatgcc aagaccatgt atttcgattc ctcgttcttt gagtgccatc gcttctccga
    29641 tggttgcgat aaacgtactg tcgattcttc tggatagatt gttttggtca ggtgcatcat
    29701 agattcttga gattagagaa gtatttcccg gccgatcttg cgtattagaa aactgagttc
    29761 cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac tttataggaa aatgttggta
    29821 caccatccgt cagcaaaacg atcacttttt tatgtccatt tggaacggat agcatgcttc
    29881 ctgcatctct tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa ggtgtaatgg
    29941 attttacttg atttttcact gaatcaaatg aacccatctg tactgcaccg ttactgtagc
    30001 tatatccttc gcttgagtag ccgacatatc ccatattgat tttgtctgtg ataccgctat
    30061 ctgctaaagt atcgacaaaa cgatcgacac caatctttac ttcaccgatc cgattattgt
    30121 cgttcatact tcctgaccag tctacgacca atacgagatc aagaggcgtg atatcttttt
    30181 gtacatttcc acgagcattc aaataaacat caaacaatcc gggtgtactt gtttcctttg
    30241 catactttcg tatcgcatag tctgcttgat taccggttcc tccatattcg atatacgaat
    30301 gggtccgatc attaggatct ccattccaag aatttatacc atcccattga tttgttccgt
    30361 ttttattgcc ttgatgatca agaacatttg tatttcctgt aggttgccaa ctggctttag
    30421 gataagtgcc ctgatcatct gttgtatatt ctgtaggaat cgtttgatta gatggcacac
    30481 tcacagctgg caaggataat gtaggcaatg taaatgggct gacagaattt tcgttttctt
    30541 ttgatgattc ttcttctatt tcgtctggct ttggtactga cacatctgta tctttcttat
    30601 tttctgtctc atcaataatg tcatattctt tattatcgac gatcagcttg tttgcctggc
    30661 tgtggtaaag tgctggatcg attggaaaac tcagatcgat agtttgttta gtgtttgatg
    30721 agatttccaa cgtaattttc ccgctattta cctgataagc atagccatct ccttcttttc
    30781 tttccacatc acttaagtaa ttttcgacta gaccaatttc tttttctatc ttttcagcac
    30841 tggtgttatt cgcatttagc ttgatgttca gtgctttttt tccagactga tccgaaatga
    30901 cttcagctga aattgtttct aaaactgttg gatcatctgt agtgtctgct attgcagata
    30961 atggcacaaa gtttgataaa ctcaatatta tcagcatgaa aataactttc agtttcttcc
    31021 ctgtggtcgt catttttccc ctcctttttc tttttttctg ttttgacgtg aatagtgaaa
    31081 attattaaaa taaacaggcg ttacataatt ttatttattt taatatgata attattatac
    31141 gaagggcgct aaagggaatc acaccaattt tcataccaat tggtttcatt tatgaagttg
    31201 attccttcga ttaatgagcg tttcattgaa agaaaagggt tgagggagaa aaacatatga
    31261 tcaggttgtt ggaaggaaac tatagaaaga tggaaaaaat cattcgaatt ctgtccattt
    31321 ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat tggtagttca gaatctacat
    31381 ttgtaaggta tatcgaagag atcaaaatcc gctggggaac ggatgtaacg attcaatctt
    31441 cacaaaaatt ggggtatcgt cttgaacatt tgaatgtacc agcttattta aatgttctga
    31501 tggaaatgtc gcaaagttct acaaacagtc gtttattaaa tgagattatt gtcaatccgg
    31561 gtaaaacgat tgagtattat tgtgaaaaaa tttccatcag ccggtcttct tttacacgta
    31621 agttgaaaca atgcaatcaa attttagaaa gctatttatt aaaagtgatc gttgaccaag
    31681 gttttcagtt aactagcctt aaaagtgagc tccagttgcg tatctttgtt acctttttct
    31741 ttttaagtta ctacggaaga cgcacgttgc catttccaat cgaaaaaaag aagatcaaag
    31801 aaattatggt caagaataat tgtcagatga atttgtttca ggaagaaagc ccatatgagc
    31861 aatctttctt tattatgtat tttgttgtgc atctcatgag agaatcccaa ggaactccat
    31921 ttgaatggaa agctggagac tcttatgtac agttgataaa tatcgataaa aaagacttct
    31981 taatcttgaa aaatctctta ggaaaattga agtacagcag ctatataaaa gtagtgaact
    32041 attttgtcaa tacgattttt tcagaatttt cggagaaaga aaaaaatgaa atcagtgagc
    32101 agatacgaaa aaatatgtcc tactgttcgt tttcatccgc tgattgcaaa gaaaaaaacg
    32161 aagaatatgt tgttcaatta ttaacgcaaa tttatttttt ctcccgtatt ttcccatacg
    32221 atacgactca tttgacgatc cgtgcaactt actttgcaca gcagatgcaa caccgccaga
    32281 tacgtctata tgctggattt cgtgcagaac ttcaatttta ttccactcta ctgaatcaga
    32341 atctgattta ttatttatct agctatatct tttggatgct tatcggtaat cctgaaattc
    32401 accattatac ttctgataaa aaaatactga ttatcagcga cttaagtctt cgacatagtc
    32461 aatatattga agaatatatc agtgatattc ttgctgtgca taaaattcac tctgagacaa
    32521 ctgctattac tgaagatcag ctgtcgaaat acaatcttct cgagtatgat ctgatcgtta
    32581 cgaatcaacc cattttgaat gcccatgttc ctcatatatt gattgatgat agtgtgtcat
    32641 tcgcaaatga agaagaactc atccgcttat tcgagttatg aataatcaaa aaacagctat
    32701 gtataaacca ccctgataaa aatttcagga caaatacaca tagctgtttc tttttattgt
    32761 attccttcag caatccaacg agatttatag cgaaagtctt ttaacaactc ttcttttgtc
    32821 caattatttg caatgagatc cgtcaaagtg ttcattacga gggtcatgga aaagtatagg
    32881 aaactaactg cttcttccga cgattgaatg gagaaatgat tgattcttaa taattccagc
    32941 catttttcgt tcattggaga atcagccgat agttctctac gtttggttgt ctgctgatca
    33001 tttcgctggg ttaacaagta taacctttgc caatagatac tttcgtgatc taagtcgcag
    33061 caccactcta aatatttttc gatccccaaa aagaaatgtt gtttgttttc gctaatgaat
    33121 tttaagatat cttgatggac tatgtgagaa ctgcttttga tcgtatacaa ataagcatct
    33181 tccaaatctt gaaaatattt ataaaaagca ccgcgcgaca tccccatctt gtcaacgatt
    33241 tctgagactt tgacttggct gatgtgctgt gagcaaaata tctccagtaa taggtcatcg
    33301 attctctgtt tctttgcgtt cgataaattg aaaaaagttt cttttggcat ccacatctct
    33361 cctttatctc tgattatacg tggatagccc ttcattgtca tgaaggattc attccttctt
    33421 tttctcttct tctgtcaagt acccatcttc cattcgataa acgcgatcac tccattgaat
    33481 catacggaca tcatgagtaa ccataatagt cgctttttgt ttctcatgag cttctttaac
    33541 tagtaatttg acgacttcat atgcatgatc tgtatccaaa ctagctgttg gttcgtctgc
    33601 taagatcaag cttggctcat tatataaagc gcgagcaatt gccactctct gtctttctcc
    33661 accagaaagg tctttaggat aactattttt aagtgactgg atatctaaag attgcagcaa
    33721 ggaatcgatt tttgttgaat cttctttttt tgaagtaact tggtcaacta gttcaaattg
    33781 ttcctttacc gttaaaaaag gaatcaaatt agagctttgg agaataaatc ctatttcttt
    33841 aaagcgtaaa tctgctcgtt ttttttctgg aagctcagtt agtgagtgac cgttgatcac
    33901 tatttctccg gaagtcggtg tttgcaatcc tcctgaaatg gtaaggaaag tacttttacc
    33961 tgaaccagaa ggaccaatga tgctgacgaa ttctccttcc tttacataaa aatcaattgc
    34021 tttcaaagct gagacctttg tatgaccaga accaaatgtt ttggtaactt ttttcatcgt
    34081 taatattttt ttcattttta ttaacctcct attgctgtaa ttgggtctac tttggttatc
    34141 gtacgtatcg aaaacaatcc gcctagcatt gcgacaatga tcaaaatccc actatatagc
    34201 aaccattgtg accaaaaaac agcaaaaggc atcgcgcttg gtaaaatcag actagtcaaa
    34261 taagcaaaca aaagagcaag cagaacgcct accaccccaa caataaagga ctgtgctact
    34321 aaagatttag cgatgaagaa atttttgatc ccttgtgctt tcattactcc aaaaatagca
    34381 gtcttctgaa gtgtcatcac gtacatgaaa ataccgacca cggcagcaac cactaagaac
    34441 aaaaagtata tcatcgcatt taaggtcata ttttgggcag aatagccagg aatactttca
    34501 atgaatgtcc caatagttaa taactgtaga cccttcgatg ctttttcttt cgaaatttta
    34561 gctgaatcct ttattacgat cccattgatc ggctgttctt cttcagaagc aaacggctgt
    34621 tgtccatact tcaaagcagt ccacgtatct agggacgcat agatgacggg agaaactgtg
    34681 taataagttt caggaaagat accgacaatc gtcaaaggat cgtcgtaact gccaattttg
    34741 atcgtatctc caattttata gcctgaatca gccagatttt gagagatgat gatttcattc
    34801 tttgttttaa attcatgccc tttcgtgagt tttggtaata aaaatgcttc tttcgttgtg
    34861 ccgaaaactg taacattttc tttgtttttt cctttgattg ctccgctgta caatccgatt
    34921 ggtgctttct ctcctccttc aatgtatttc agatctcctc ttgttaattg tgaagctgca
    34981 aatacttgat ttgcatcctc cgacaaaaca atctcttgtg cgtcccaatc atcgattgct
    35041 tttttaaact cttctgaaag tccattcgct agtcctgaca acatgaatac gacatacgca
    35101 atcaagaaca taataccgac gatcaagcca tatcgtaatt tagaatagcg aatttctttg
    35161 atagctagaa acatttgttt ttcctcccta tgatagattg atagtgacaa aatgtcactt
    35221 gttgaatagt gtaactctaa agtgacaatt tgtcacttgt gttgtttttt tcttatcaat
    35281 aaattgtcta agacatcttt cgataaaacc ttaacatgag tcataaaaaa tttatttaag
    35341 ccttatttat gctttccatg ataaactaaa gtgaccaaat caatgaggtg ggaaaatgat
    35401 taaactatta gtagtagaag acgataaagt attatccaat aatataaaag aaatactcgc
    35461 tgcaatcggc gagataacac aagtttatga tggtagtgaa ggactgttcg agttggaaag
    35521 tggagtttat gatcttgcaa tcttggattt gatgatgccg gaaatgaatg gatatgaagt
    35581 gttagcaaaa atacggaaag aaaaaataca gactccagta ttgattttga ctgcaaaaga
    35641 tagtttggaa gataagattg aaggatttga acgaggtgca gatgattatc taacaaaacc
    35701 tttctatcga gaagaactga tcatgcgcgt gaaagcatta ttgaaacgct cgctaggtct
    35761 ctttgatgaa aatgttctga agtataagga tatcagttgt gatttaaaaa gcaatgaagt
    35821 cttgtataaa gaacaagttt tgcccataca aggaaaagag tttgaattgc tcgtttattt
    35881 tttacaaaat aagggaatca ttttgacaaa agagcagata tttgaccgga tctggggatt
    35941 tgattctgat acgacgatta cagttattga agtttatatg agcaacttac gaaaacatct
    36001 gcgaagtact ggattggata aagaaatcaa aaccttacgt aatgtcggtt atatactgca
    36061 aagggtgtga tgcaatgaat ggagatatta cacgaaagca acgtagtcgc ttttttgcga
    36121 caaatgtagc agcatttgct tttatctttt ttcttttggg attcattact ttacagatat
    36181 taaaccaatc tgcatacaga gaaacagatc tgtcattaga gaaaataaat tttgattctc
    36241 gaacagtaca attagagata caaagatatc agcaaggcga tcccttttta aacccgactc
    36301 aaacggaaca aaacagaaca aacatggaac agccattaat ggaaccatca aatgaccgtt
    36361 ttaacactca agtgatcttg tggtctaaaa atggtgagat attgaataaa gcagctattg
    36421 gtgggcgttt caaccaaatc aaagaattaa aaatggatac gaaaaatctg aataccatcc
    36481 aaaatattga attaaatgaa gacagtggct catctgccct ctctttccgt tcgattacga
    36541 aaaaagcgga gacgaatcaa gatggcgttg cctacatcca aattttagaa aatacaaatc
    36601 aaatcaagaa ttcattagcg acttttcgca cgattatgat tttgtgtatg attgtttttt
    36661 ggattctctc tattggtatc agttattact tgtctagtct aagcatgcgc cctattattc
    36721 tttcttggag aagacaaaaa gaatttgttg agaatgcttc tcatgaattg cgcacaccgt
    36781 taacgattat tcaaaatagt ttggaacatc tttttaccaa gcctaatcat actattattg
    36841 aagaatccga aagcatcgct caagcattgt cagagacgag gcgcctcact ggattgactt
    36901 cggatttgct gacaatcgct cggaatgatt ctgatcagca gctgctttca aagcaaatga
    36961 tcaatacaca agaatatatc gaacaacttg ttaaaccatt tcaagagatg gccatcatag
    37021 atggaaaaga gtttattctc cataacgatg caacattaga agtaccaatt gatgaaaaaa
    37081 aaatccatca agttttggtt attttgctgg ataatgcatt aaaatatacg aatccagaag
    37141 ataagatcat tttggattca tctgttaacc ataaagaatg gctgattcac gtaaagaata
    37201 ctggcccttc tatcagtgaa gaagatcaag caaggatttt tgatcgattt tatcgggaag
    37261 atcaatctcg ggcgaaagag actggcggat acgggcttgg cttggcaatt gcaaaacagg
    37321 taatcgaaca gcatcatgga cagatttctg tatccaatta tcttccacaa ggagtcgatt
    37381 ttaaagtcca attgccgttg aagcctaaaa aataatgctt atccctaaaa aagggctgtg
    37441 acaaaaatct tgtcacagcc cttttttgtt gcgaataaac ggtgttccag aagtagcttc
    37501 ttcggaaata agccgaaatt tctttaagct gaccacttct gtcacaacct ctttagctta
    37561 cgcttacgat tttttattgc ggtcttcaat gtgtacatca ccgcttgtgt caatatccaa
    37621 ttcttctcgg ctaacatctt ctgtcacttt ttcaacatct tcatgttctt ctttatgaat
    37681 cccaacttct tcacgaataa ctgtatgttt gcttacatct acttgctctt ctttgattgg
    37741 gatagtaatc gtgtcatctt ccaatttttc gtctgttccc tgtgaagatt gatctgttac
    37801 tggtttgcgt tcgatgacga tttcttcacg tttgaccggt acttctaccg tttcagtatc
    37861 gttgaccaca tgtttgtgga tgtcgacttc tccagttgtc acatcatgtg tatttacatc
    37921 taatttttct tcttttagtt gaattgtatc atcatctaga tttggtttgc gatcgatgtc
    37981 ttcagtcttg cttgtaccca ttgtatcagt ctcgccaggt acgtccatcg gatcagttcc
    38041 tggtgtcata ttcaatggat ccgttgtatt ttgaaagcct gtcgtgccac ctacgtccat
    38101 tggatctgtt ccagtgccag tttctaatcc agtccccatt gtgcttgctg ggctaacagt
    38161 atcttgtgta tccattgtat cttctgttgt ttctggctga cgatagcctt ttactgtgat
    38221 gacaaatttt ccatcagaaa tatctcctct atattgatat aaaggatcat tttctggtgt
    38281 agccgtctca gaatcatagg tatcaaaaga aaaagtatct tttatttttt cccataaaga
    38341 acggtcttct tcaccatcca tccgattttc atctgtttca acatctatac cagacaatcc
    38401 gtcaaatcct agtctagatg ctgcttcatc agtagtataa ataacgatat catctcgttc
    38461 atacccttcg ttacgcaacc gttcaattac tgataatgct tcttccctcg tactatagct
    38521 tccgatgacc gttaaatttt tctcttccat gtaaattcct cctagcaaat agatttatga
    38581 ttggtatatt gttacttata aatttattct ataggagacg attgttttct acaaatattt
    38641 agattggaat ttgattaaaa caatttaatt gaaatttgaa aacatttatg ttaatgaaag
    38701 tcatattcta ataaaatgtc atttgtttct atatgaattt ttatgaaaaa tttcatataa
    38761 tttccactcg ttgtgatggt ttaatt
//
LOCUS       DACDKJ010000020        37489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDKJ010000020 DACDKJ010000000
VERSION     DACDKJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.020"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            603..1931
                     /locus_tag="IV328_001171"
     CDS             603..1931
                     /locus_tag="IV328_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8937328.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2133..2966
                     /locus_tag="IV328_001172"
     CDS             2133..2966
                     /locus_tag="IV328_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8937329.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2982..3719
                     /locus_tag="IV328_001173"
     CDS             2982..3719
                     /locus_tag="IV328_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8937330.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3874..4800)
                     /locus_tag="IV328_001174"
     CDS             complement(3874..4800)
                     /locus_tag="IV328_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8937331.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5075..5908
                     /locus_tag="IV328_001175"
     CDS             5075..5908
                     /locus_tag="IV328_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8937332.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5942..6781
                     /locus_tag="IV328_001176"
     CDS             5942..6781
                     /locus_tag="IV328_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8937333.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6807..7367
                     /locus_tag="IV328_001177"
     CDS             6807..7367
                     /locus_tag="IV328_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8937334.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7566..9287
                     /locus_tag="IV328_001178"
     CDS             7566..9287
                     /locus_tag="IV328_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8937335.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9452..10594
                     /locus_tag="IV328_001179"
     CDS             9452..10594
                     /locus_tag="IV328_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8937336.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10610..11821
                     /gene="thiI"
                     /locus_tag="IV328_001180"
     CDS             10610..11821
                     /gene="thiI"
                     /locus_tag="IV328_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8937337.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12394..13041
                     /locus_tag="IV328_001181"
     CDS             12394..13041
                     /locus_tag="IV328_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8937338.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13434..16079
                     /locus_tag="IV328_001182"
     CDS             13434..16079
                     /locus_tag="IV328_001182"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8937339.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16389..17702
                     /locus_tag="IV328_001183"
     CDS             16389..17702
                     /locus_tag="IV328_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8937340.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17692..18345
                     /locus_tag="IV328_001184"
     CDS             17692..18345
                     /locus_tag="IV328_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8937341.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18400..18951
                     /gene="radC"
                     /locus_tag="IV328_001185"
                     /pseudo
     CDS             18400..18951
                     /gene="radC"
                     /locus_tag="IV328_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19082..20167
                     /locus_tag="IV328_001186"
     CDS             19082..20167
                     /locus_tag="IV328_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8937342.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20164..22056
                     /locus_tag="IV328_001187"
     CDS             20164..22056
                     /locus_tag="IV328_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8937343.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22063..22440
                     /locus_tag="IV328_001188"
     CDS             22063..22440
                     /locus_tag="IV328_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8937344.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            22594..23376
                     /locus_tag="IV328_001189"
     CDS             22594..23376
                     /locus_tag="IV328_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8937345.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(23502..24233)
                     /gene="erm(A)"
                     /locus_tag="IV328_001190"
     CDS             complement(23502..24233)
                     /gene="erm(A)"
                     /locus_tag="IV328_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8937346.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(24291..24378)
                     /locus_tag="IV328_001191"
                     /pseudo
     CDS             complement(24291..24378)
                     /locus_tag="IV328_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            24742..25404
                     /locus_tag="IV328_001192"
     CDS             24742..25404
                     /locus_tag="IV328_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8937347.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            25639..25782
                     /locus_tag="IV328_001193"
                     /pseudo
     CDS             25639..25782
                     /locus_tag="IV328_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            25932..26132
                     /locus_tag="IV328_001194"
     CDS             25932..26132
                     /locus_tag="IV328_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8937348.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(26386..28065)
                     /locus_tag="IV328_001195"
     CDS             complement(26386..28065)
                     /locus_tag="IV328_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8937349.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(28067..28279)
                     /locus_tag="IV328_001196"
     CDS             complement(28067..28279)
                     /locus_tag="IV328_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8937350.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            28672..30402
                     /gene="efrA"
                     /locus_tag="IV328_001197"
     CDS             28672..30402
                     /gene="efrA"
                     /locus_tag="IV328_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8937351.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            30399..32159
                     /gene="efrB"
                     /locus_tag="IV328_001198"
     CDS             30399..32159
                     /gene="efrB"
                     /locus_tag="IV328_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8937352.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            32254..32451
                     /locus_tag="IV328_001199"
     CDS             32254..32451
                     /locus_tag="IV328_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937353.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            32604..33089
                     /locus_tag="IV328_001200"
     CDS             32604..33089
                     /locus_tag="IV328_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8937354.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(33193..33786)
                     /gene="clpP"
                     /locus_tag="IV328_001201"
     CDS             complement(33193..33786)
                     /gene="clpP"
                     /locus_tag="IV328_001201"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8937355.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            33966..34493
                     /locus_tag="IV328_001202"
     CDS             33966..34493
                     /locus_tag="IV328_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8937356.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            34584..35321
                     /locus_tag="IV328_001203"
     CDS             34584..35321
                     /locus_tag="IV328_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8937357.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            35325..36242
                     /gene="miaA"
                     /locus_tag="IV328_001204"
     CDS             35325..36242
                     /gene="miaA"
                     /locus_tag="IV328_001204"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8937358.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            36244..37473
                     /gene="hflX"
                     /locus_tag="IV328_001205"
     CDS             36244..37473
                     /gene="hflX"
                     /locus_tag="IV328_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8937359.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        12579 a         6171 c         7846 g        10893 t
ORIGIN      
        1 ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa tcacttgggt ttctttttct
       61 tttattatta tgatgaatca agctttctgt ttttgtcacg tatgttgtaa tctttacgaa
      121 agagtagagt tggctactca taatcaacca tacttcccat catcgctaaa tacgaaataa
      181 atcagcattc ctacaccaat caaagcacaa gctgcaccaa gaaaagggat gacattccca
      241 aagttatctg tattcataag tatgccacca cccacaaaca aaaagccgcc agcaatcagg
      301 tttttcattt tattcatctc ctttcttacg ttatagtatt tccttctttc aatgagtatt
      361 ttgaaagcta tcttattgta tgatgttctt ctaggcctct tctttattat acaggccatt
      421 ttcgttatct catagaaata ttccttttta tctgaaaact attgtatacc gtatgacatt
      481 tttcttcatg aagttgtaag tatattctca ttcaaatgaa aatttcatca tagaagagat
      541 tgtatttcta aattcttaag agtaaaatac gaagtataac ttggatttgg aaggatgatt
      601 ggatgaaagc tattacgcct gatatgacaa caaaatttaa caaacagttt caagcaaata
      661 tcaaacagca ggcattgcag cgggcagttg tgaagaacgg aattactgct tctgctgaaa
      721 atgtttcagc acatgtggag aatacaccgg tattttctat agatttggct acggggaaag
      781 tggctaatca aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac acattccgtc
      841 ataagatgct gaatcaattc catttaaaag agtttgaatt atcacaaaat tacacttttt
      901 tctgggataa atatgaaaag gcaaattatt tctatgaaaa catcattgca acaggaaaag
      961 aggcaatcga tagccggaaa gtagctttct tactcgctac tcctcaacaa gatggcggcc
     1021 aatgggatat gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa tcggtgatgc
     1081 cggaaagtgc gaatagttca aattctcgag atttgaataa ctacttgaac aaattactgc
     1141 gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg agaaacatta gacgctattg
     1201 aagaaaagaa agaagaaatg ctagaaagca tttataactt cttagctatc tcattaggaa
     1261 cacctccaaa agaattcgat tttgagtacc gcgatgaaga aaagaactat catcttgacc
     1321 gaggactgac accacaaatt ttttatgata aatacatcgg tgtaaaatta gatgattatg
     1381 ttagcgtgat caatgcacca acaaaagaca aaccattcaa tcgttcttat acagtagaga
     1441 tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct gaatgttgat atggaaactt
     1501 tcaaaaaatt agcgattgct cagcttgaag aaggagaatc tgtttggttt ggttgtgatg
     1561 taggtcaatc ttctactaga actagtggca tcatggcctt agatgcttat tcaatggatg
     1621 atctatttga tacggatttt acaatgacta aagctgaacg tttagatttt ggagaaagtc
     1681 tgatgaccca tgcgatggta ttgaccggtg ttgacttagt agacgggcaa tcaacaaaat
     1741 ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt gttatgagtg
     1801 atgcctggat ggacgaatat acgtaccaaa tcgttgtaag aaaagattta ttgacgaaag
     1861 agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat ccaatgggtg
     1921 ctttagcata gacgagtatt tcaatgaaga gtatgtagta aagataaaaa gagctggctt
     1981 aagccagctc tttttatctt taaagtccat tccctgtttt ttatggtccc tgtgctataa
     2041 ttctaaataa aggataagga ttggaaataa tccttttatc attaaaaata tgatgacgaa
     2101 gtagaataaa attgggagta ggggggaaag tcatggatat ccaaactgtt ttatctgatc
     2161 tgaaattaaa tggtaaagtg attccagttg ttggtggcga tgttaatcag acctatcgta
     2221 taaaaaccga acatcgtgct tattttttaa aaatccatcc gaatgtaaaa aaaggatttt
     2281 ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt cgtacgtgta ccggatacgt
     2341 atatgctagg tgaaacaagt gaaggcgctt atttgttaat ggaatggatc gagccgggaa
     2401 aaggggatca acgagatctt gctgcagctt tagctaatct ccatcagcaa acagcgccac
     2461 aatttggttt tcgtaaagat aactatttgg gtacacttgt tcagaaaaat tcttttgaag
     2521 aagattggtg gacttttttc tttaaagatc ggcttgaatc ccagatttct ttggcagaag
     2581 aaacgaatcg atggaatgta cagcgtcaag aaaagtatct gcgatttaaa gaacgtgtat
     2641 tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt gcacggagac ctgtggagtg
     2701 gtaatgtgtt ctttgaccag caagggcatc cagtttttgt cgacccagct gtttcttatg
     2761 ggaatcggga acaagatatt gcaatgagcc agttattcgg tggtttccga ccggaatttt
     2821 tagatgctta tcaaactata tttccattag aaaagggatg gaaagaccga ctgccgattt
     2881 accagttata ttatcttttg gcacatttga atatgtttgg agaaagttac ggttcccaag
     2941 tagatcaatt actagaaaat ttttaagagg agggcgtgtt tttggcaaaa aaatatacca
     3001 aaaagcatga agtttcatat tatgagtgtg acatcaacca aacgatgaca tttccttcga
     3061 tgttaggagt agttatcaaa acgtctgaag atcaaagcga ccagttggga agaggcagtg
     3121 aattcgtcaa tagctttgga cttacttggg tcatcacgaa ttattccatg gagattaatc
     3181 gtctccctag agtcggtgaa gtaatctcag taacgactca agccatggag tacaataagt
     3241 ttttctgtta tcggaacttc tggatccatg atgaagatgg gaaagaatta gtcaaaatag
     3301 aatctgtttt cgtattgatg gattttgtga atcgaaaaat gagcagtgtt aatgaagaga
     3361 tcattgctcc ttttgagagt gagaagatca agaaaatcaa gcgtcaggaa aaaattgaaa
     3421 agattgattc gggcttcatg ttgccatatc gtgttcgttt ttatgatatc gatagtaatc
     3481 agcatgttaa taatgcgatg tattttaatt ggatcattga cgtattagga tatgactttt
     3541 tgactacgca tgttccagaa aaagtaatta ttcgatttga taaagaagta gagtacggga
     3601 atgagatcga aagccatttt gaacaaatcc ctatcgaaaa tggtgtcaaa acaaaacatg
     3661 aaatccgaat gcaagaacag ctgtattgtg aagcaaatat tatatggaaa aataaataaa
     3721 aaagaggttg tgacagaagg gggcaacttc aagaaataag aagaaattta cgaaaattgt
     3781 ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga agctattttt gtcacagcct
     3841 ctctttttaa gtttagctat tagataatga tgtttagttt tggataaacg aaatcaattc
     3901 ttcacccgac aaaccatagt ttacagcaat caatagtttt aaacgtgctt tttgactatt
     3961 aatactgtta cagaaaataa tccccatttt ttctagttct tttcccccgc cttcataatc
     4021 ataaacaggc tctgcgattc cattgaaaca acgagaaaca agtactacag ggattttttg
     4081 gacaagaagc ttttccaaag cagctaaggc ttgtggaggg agattccccg cacctaaagc
     4141 ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct tccaataagt ccccttgcat
     4201 cccagcatat gctttaacga tcgggatagt cccatctaca gcggaaatgt ccagtcgctt
     4261 tgtttctaaa agttcctgca agaataaaat tcgattctta gttactaagc cgatgggacc
     4321 caatgtaggt gttcggaaag tagcaacatt tgtggtatgg gtctttgtca cgtagcgtgc
     4381 agaatggatc tcatcgttca tgaccaccaa aaccccctta tctagcgctt cttcacaact
     4441 tgcgacacga atcgcacttt caaaattata aaggccgtca cttccaagtt cattacttga
     4501 acgcattgcc ccagtcaaaa caattggtaa tttgttgcca atcgttgtat caagaaaaaa
     4561 agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc actccggata tacctgatgc
     4621 ataagcggac aagatacgtt cttttaatac caacatgtga gaaggcatga tgtgtggtga
     4681 gggaagattg aagatatctt caactaataa ttctacatgc ttggggattg tgatttctgc
     4741 atctaataat gggtttgcca catcaggact aacaccgccc gtgagcatgt tttctttcat
     4801 ggcgatcgtt ccgccggtgt gtagtacaag tatttttttt cattttacag acttccttac
     4861 gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt tttgctatca
     4921 gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta aaatggaaaa
     4981 gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt tacaagaaag
     5041 cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat taacatcgtt
     5101 taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat atgcagggtt
     5161 tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt cctcagttga
     5221 gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat
     5281 cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga ttgcgcattt
     5341 tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt tagtccaatt
     5401 cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg
     5461 gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca gcgatgaatt
     5521 tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca tcatcgatga
     5581 gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc cgaactttgc
     5641 tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata aagagtggcg
     5701 taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag cacatgaagt
     5761 caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata attctggtgg
     5821 tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc cagatggact
     5881 caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag gggcgaagtc
     5941 tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc gttcaaaagc
     6001 gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt tatgcggtgt
     6061 agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg aattagatat
     6121 gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta tcgaccaccc
     6181 ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc atcgacaaat
     6241 cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag gacaatcgat
     6301 ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa
     6361 aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt
     6421 aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga aaaagttaga
     6481 acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag atatcatccc
     6541 aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg aaattgaaat
     6601 ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga aaggtgtagg
     6661 aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag actttgtgac
     6721 tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc tgatttatta
     6781 gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa aaagtagaaa
     6841 cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt acagaaaatg
     6901 aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt gcagctactt
     6961 cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca ttagataaag
     7021 cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt gaacaagtag
     7081 atgcactgac agatatcctt tactttactt atggttcttt ttcattacta ggaatagtgc
     7141 ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt ccagatggaa
     7201 aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg cagatggaat
     7261 atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa gcgattgcta
     7321 agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca gagtttcact
     7381 atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt agtgaatgca
     7441 tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg tattatttgg
     7501 atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa acggaggaac
     7561 cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata gtcgccattg
     7621 tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg gatgccttag
     7681 aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat gatgttaaaa
     7741 aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa aaatggaatg
     7801 acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg gaaaacctca
     7861 atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg
     7921 accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg
     7981 aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa
     8041 aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa aaacagatca
     8101 aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga gaccctgttg
     8161 aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa gctctgatga
     8221 aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa cttgaagaaa
     8281 tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa gaagatcttg
     8341 ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa
     8401 cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt gattcgttat
     8461 atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac caatcaacga
     8521 ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt gagctggatc
     8581 acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa
     8641 ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc cgtcaacacg
     8701 aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc ttagaagata
     8761 tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa ggcgaaaaag
     8821 aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa cgttttgtag
     8881 aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt gttgctactg
     8941 accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg gaagaggtca
     9001 atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag acacatgatt
     9061 tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt tatcgtcata
     9121 cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat aaagaatatc
     9181 actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa cctggtgctt
     9241 tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc aaaaaagtta
     9301 gtaaaccagc aagagcaaga cggattattg tcttgttctt gtcttttttt gtatatttga
     9361 aaattgaaac aagtgaaaat ttcacctgct cttgaaagca ccttggacaa ccgttataat
     9421 agaaaagaat ttcaaaaaag aggggtttct tatgatatat tttgataaca gtgcaacaac
     9481 cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga ttattgggaa
     9541 tccatcaagc ttgcatgatt taggcaatca agctaaccgg ttgttgcagc aagcaagaaa
     9601 gcagatcgct gatttgatcc atgtagaacc agaagaaatc tattttactt caggcggaac
     9661 agaaggagat aactgggtat taaaaggaac gatgatcgaa aaaagagaat atgggaatca
     9721 tatgatcatt tcaggagtcg aacatccggc cgtcagcgag acagcagaac aattaaaaga
     9781 attaggaatc gaagtaagta tcgccccagt ggataaacgc ggatttgtac gtgtcgacga
     9841 attgaaagaa ttaataagaa aagagacagt attagtctct gttatggctg tgaataatga
     9901 agtaggatcg attcagccca tccaggaaat cagtgatctt ttggatgatt ttcctaaaat
     9961 tcatttccat gtagatgctg ttcaggcgat tggaaaagta ccattctcgc aatggttgac
    10021 agaaagagtt gattttgcaa cattttcagc tcataaattc catggcccac ggggaactgg
    10081 tttcatttat tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga
    10141 aagaaatcag cgaagtggga cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt
    10201 acgtttatca ttagagataa aggaacaaaa acctgagcag acacaacgct taaaagtagc
    10261 tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag gaactgacta
    10321 tgcaccgcat attttatgtt ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc
    10381 cttagaagaa aaacaaatat ttgtttctac tactagtgcc tgctcctcac gtaaacatat
    10441 ggcaagcagt acattgcacg caatgcatgt accaaatgat ctggctacat ctgctgttcg
    10501 aatcagctta gatgaaaaca attcgcttgc agaagtcgag caatttatga ttattttcaa
    10561 gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag
    10621 tgaaattatg gtccgttatg gggaactttc gacaaaagga aagaatcgaa aaagctttat
    10681 catgcaacta gcacaaaatg tcagacaagc tttgaaagac tttccagaaa tcaaaatcca
    10741 tgctgatcgg gaccggatgc atcttttatt gaatggcgca gacgggcagc tagttattcc
    10801 aaaactagca aaaatatttg ggatccaaaa tttctctcca agtattcgtg tagaaaaaga
    10861 tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa gaaatttata ctgggaaaga
    10921 aacatttaaa attatcgcta aaagaagcga tcaccaattt gaattgacaa gtaatgaatt
    10981 gaatcaaaca ttaggtaatg ctgtttttga tattttcccg catatccaag ttcaaatgcg
    11041 tcaaccagat attccattac gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga
    11101 aacgatcaag ggagctggag gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct
    11161 ttctgggggg attgattctc ctgtagcagg gtactttgcc atgaagcggg gagtggaaat
    11221 tgaagctgtt cattttgcta gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa
    11281 agatctaaca gccaaactag caccttatgt gggaacgatc cattttatcg aagtgccatt
    11341 taccgagatt caagaagaaa tcaaaaagtc agtgccacaa ggatattgga tgacaattac
    11401 tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt attcgtcatg gtttagtcat
    11461 tttgaatggt gaatcattag gtcaagtggc ttcacaaact ttgcaaagta tggtggctat
    11521 caatgaagta acgaatacgc caatcattcg gcctgtagcg acaatggata aacttgaaat
    11581 cattgaagta gcagaacaga tcgatacatt taatctttct atccagcctt ttgaagattg
    11641 ctgtacgatt tttgctcctc cacagccaaa aacacgccct caactggaaa aggtccatca
    11701 atatgaagag cgtttagcaa tagatgaaat gatccaacga gcactagctg gcttgaagat
    11761 ggaagagata caagcagaat cgtctaatga aaaaactgaa gaattttcag aattgctata
    11821 agaggttatg aaaatagtgt tttgaccaga gtattagaga aacaaaacga aaaaatagct
    11881 ccatgtattt taccgttttt gaactaatgt cgaagattag ctgattgaat accatttatc
    11941 aagaagttat ggaaaagccg gttctttttt actaactgaa aaataaaatt ttatttagag
    12001 atcaagctac agttgataaa tgatataaaa aagagaattt cccatgaaat agcgaggttg
    12061 tgacagaagt gggcagcttc aagaaataag aaggaattta cgaaaattgc ttttcaaatt
    12121 tttgtgaaat ttcggcttat ttttcgaaga agttacttct ggaacaccgt gtatcagagg
    12181 ttgcgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
    12241 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gttttatttg
    12301 tctcacaccc ttttccttcc tacttgtaac cttgctcccg tgttagaaac atgttacact
    12361 aaatatgtga aattattaac aaggagcgtc gttatgcgag aaaaaacaga tacactgcca
    12421 aaagcaactg cgaaaagatt acctttatat ttacgctact tgaagatgct ggatgactca
    12481 ggtataagta gaatcaaatc aaatgagttc agcgaaatca cccaaattcc ttctgcaacg
    12541 atccgcagag acttctcgca attaggcgag ctaggcagaa gcggatatgg ctatgatgtc
    12601 ccttttctta tcgatgtatt caataatatc cttaatacta aagaagaaaa acgaatcgca
    12661 ttagtaggat atggaaatct tggcaaagca ttgaaacaca acaattttag aagaaatgaa
    12721 aatctaaata tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg agtaattgat
    12781 ggagaaatga tctatccgat tgatcgtttc gcggagatag ccaaagcaaa aaatgtgaca
    12841 gtagcaatct ctacggtacc aagtaaatat tcacaatctg ctattgatga aattgtcaaa
    12901 ggaaatgtaa ccgctatact taattttgcg cctgacagag taactgtccc agcttatgtc
    12961 aatgttcaat acattgattt gacaaccgag ttgcagacgc ttatttactt tgatgagaat
    13021 tactcagaag ttttttctta ggaaaatttg agtaggctta ttgatcttgc gagaactcgt
    13081 cgtaattctt tcaaaaattt tttagtacta aatgaagtgg ttttttcacg accacttgtt
    13141 gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct gaacaagagg agtaactggc
    13201 acattcattc ttagagagag aactatctgc tgaaaggttc tcaatgacaa ccagtgaagg
    13261 tagcttggga gtttctgtct tgaatggagt agagatagac gagtcgtgat cgttaactca
    13321 cttgagaggc aataatgatt gattattgca atttaggtgg taccgcgtaa atcatacgtc
    13381 ctagaatacg aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac agcatgacgg
    13441 aagaaaataa cctatcaaca aaatataatc cgcaagaagt tgaagctgga cgttatcagc
    13501 attggttaga acaagatttg tttaaaccga gcggagataa aaaagcaaaa ccttattcaa
    13561 tcgtaatccc gcccccaaat gttactggaa aacttcattt agggcatgct tgggatacga
    13621 ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg atatgatact ttatggctgc
    13681 ctggtatgga ccatgcagga atcgcgacac aagcaaaagt agaagcgaag cttagagagc
    13741 aagggatttc tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg tgggaatgga
    13801 aagaagagta tgcgagccac atccgcgaac agtgggctaa aatgggctta tctttagatt
    13861 acagtaggga acgttttaca ttagatgatg gattatctga agcagtacgt aaagtttttg
    13921 ttaccttata cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac tgggatcctc
    13981 aagcacgaac agctctatct gatatcgaag ttattcataa agatatcgaa ggcgcctttt
    14041 atcatatgag ctatgaacta gcagatggat caggcgtagt agaaattgcg acaacccgtc
    14101 cagaaacgat gttgggagat acggcgattg ctgttcatcc agaagacgag cgttatcaag
    14161 cgctcatcgg caaaaaagtg attctacctt tagtcaataa acaaatccca atcattgcag
    14221 atgaatatgt agatatggaa ttcgggacag gtgtagtaaa aatcactcca gcacatgatc
    14281 caaacgactt tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt atggatgatg
    14341 atggtacgat gaatgaacta gcaggaaaat atgctggaat ggaacgtttt gctgctcgta
    14401 aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat taaaatcgat acgatgatcc
    14461 acagtgtggg tcattccgag cggacaggtg tcgttgttga acctcggctg tctactcaat
    14521 ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga taaccaagaa acagatcaag
    14581 cagtggaatt cttccctcca agattcaatc aaacgttttt acgttggatg gaaaatgtcc
    14641 atgattgggt aatctcacga caattatggt ggggacatca aattcctgca tggtatcaca
    14701 aagaaacagg tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa aactgggtac
    14761 aagatcctga tgttttagac acttggttta gttcagcttt gtggcctttc tcaacaatgg
    14821 gctggccaga tacggaaagc gaagattata agcgatactt ccctactagc actttagtaa
    14881 ccggttatga catcattttc ttctgggtaa gcagaatgat tttccaaagc ttggaattta
    14941 ctggcgaacg tccatttgag aatgtcttga ttcatggatt gattcgtgac gaacaagggc
    15001 ggaagatgag taaatcatta ggtaatggaa tcgatccgat ggaagtaatc gaaaagtatg
    15061 gagcagatgc tctccgctgg ttcttatcga atggttctgc accaggacaa gatgtccgct
    15121 tcagctatga aaaaatggat gcttcttgga actttatcaa caaaatctgg aatgcttcac
    15181 gtttcgttat catgaatatc gaaggaatga ccgtagaaga gatcgattta tctggagaaa
    15241 aatcagtggc tgaccggtgg atccttactc gtttgaatga aacgatcgaa cgagtaactg
    15301 agctatttga tcgttttgaa tttggtgaag caggtcgaca actgtataac tttatctggg
    15361 atgatttttg tgactggtac atcgaaatga gtaaagaagt actttatgga gatgatgctg
    15421 tttcaaaaca aaccacccgt agcatcttag tccatacact tgatcagatc ctacgtttat
    15481 tgcatccaat catgccattc gttacagaag aaatatggga acatattcct catcagggaa
    15541 attctctagt agtggcagaa tatcctgttg tacgtccaga gtttgatgat gaaacagctt
    15601 ctaaagggat ggaagtattg aaagaattga ttcgagccgt tcgaaatatt cgttcagaag
    15661 tgaatacacc attgtctaaa ccaattacgt tgctaattaa aacaaacgat gctaaaatcg
    15721 atcaattttt agttgaaaat acaagctata tcgaacgctt ctgtaatccc gaagagttga
    15781 cgatatcaag tgagatcact gcgccagatc tagcgatgtc agctgttctg acaggtgcag
    15841 aaatcttctt gccgttagca ggattgatca atattgaaga agaaatcaaa cgattagaaa
    15901 aagaactgga aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt tctaatgaac
    15961 gttttgtttc taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa gaaaaagatt
    16021 atttagagaa gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact attcaataag
    16081 aatagaaaaa gagagtgtga aatcagctag ctattactaa aagctagctg attgaacatc
    16141 gttacaggaa agagtttgag cctcactctt agcttcgatt cataaggaga tgtgacataa
    16201 attttgtcgc atctcttttt tagtctactc ttattatgta ggagaaggag ggaggtggat
    16261 catgtatcaa gagtcaataa agtagttgat aaaatcgttc ctctctctgt ttcttttctc
    16321 tttgtcatac ttttattttt cttatgtgtt ctatttgtta taataaaaga gaaatggggg
    16381 aattgttgat gacgattgaa gaagcaatag aatggatcca tagtcgatta ccatttggtt
    16441 cacgtcctgg ccttgaccga gtcgaagctt tattagaagt ggtagggcat ccagagagaa
    16501 aagtaaaaac gatccatatc gctggaacaa acggaaaagg ttctactgta gcttatttga
    16561 gaagcctatt agaagaggct gggctgacag taggtacatt tacttcacct tatatcgaaa
    16621 cgttcaatga acggatcgca atcaatggca aaagcattcc agacaatgat ttgatccagc
    16681 ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca atttgaagca gtagctggta
    16741 ttactgaatt cgagacatta acagcaatgg catttgatta ttttttgatg aagaaagtgg
    16801 acgtagcgat tatcgaagtt ggcttaggcg gcttattgga ttctaccaat gtagctgtac
    16861 caatgctgac gggaataaca acgattggac ttgatcatac cgaaatactt ggagatacaa
    16921 ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca gtagtaacag
    16981 gaaatattgc cccagaagct tttactgtca tccaacaaac agctaaagca aaaaatgcac
    17041 cgctttatgt atacggggaa aattataaaa taaactatct gcatcctgat gagatgtggg
    17101 gagaagtatt tgaatttttt aatgaaatag gaaagatccc tcaattaacc actccgcttt
    17161 tagggcaaca tcaagctgaa aatgcaggag tagccatcga attgtatttt gtttactgtc
    17221 aattggaaaa gctgcctttc cgagaaaaag atgttttgaa gggattgaag aaatctcgat
    17281 ggccaggaag aatggagcgt ttgagtaacg agccattagt tgttttagat ggtgcccata
    17341 atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa ggatatcaaa
    17401 tcaacatttt gttttccgcc ttacgaacta aaaatgttag agaaatgcta gaacgattgc
    17461 agcaagtacc aaatgtccat atttatttga cgacctttga acatccaaaa gccatcgaat
    17521 taagtgaagt atcagattta gcaagtgaga gagtatcaat cgtttcattg tggcaattcg
    17581 gattagcaga tatattggaa aagatgacaa atgaagacgt attagttgtg accggttcac
    17641 tttatttcat ttcagaagtt cgccatttat taatacaatt aggaggagat catgaagtat
    17701 aatgcagtga tttttgacat ggatggtttg ctgttcgaca cagagttagt gtattacgaa
    17761 gccacacaag tagtagcaga caagatgggt tttccttatg ataaagacct gtatttaaaa
    17821 ttcttaggtg tttcagatga agaagtatgg gcgaattatc atatgatttt ctcggaattt
    17881 ggcaaaacaa gtgtacaaag attcatagat gattcatacc aggaaacgct tgaacgtttt
    17941 tcgcaaggag cggtacaaat gaagcccggt gtgttggaac tcctctcttt tttagaagaa
    18001 catcagattc ccaaagttgt cgcttccagt aaccaacgag cagtcattga acttttattg
    18061 aagaagaaca atctgtttga acgatttgag acgattgttt ctgcagaaaa tgttcaacga
    18121 gcaaaaccag acccagaaat tttcttgtta gctaatcaga agctaggtac agcgaagaaa
    18181 gaaacattga ttttagaaga ttcacaaaat ggtattttag cagcttctgc ggctgagatc
    18241 ccggtagtca tggtaccaga tctgcttcca ccatcccaag aattgatcaa aaaaacagct
    18301 ggggttgttt cttctttaca tgaaatccct gcattgttaa aataaaaatc catctctttt
    18361 cgtatcttat tttagataag atagaaagga gatgtttttt tgggaaaacg attgatcaaa
    18421 gaagtaccta ctagctcttt accaagggaa cgtatggaaa tttatggcgc ggaagcttta
    18481 tcggatcagg agcttttggc aatcttactg cgtaccggtc aacaccctta tagtgtgatg
    18541 tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg cctctttacg acaggctact
    18601 ttgcatgagt tagaagagat tcgagggata ggacgcgtga aagcaatcga aatcaaagcc
    18661 ttgatcgaat taggtcgacg aattcagacg gatcatgaaa gagcggctcc accggttcgt
    18721 accagctacg aattggccca gcaattgatt ctggaaatga aagatcacaa acaagaacat
    18781 ttggtttgta tttatttgga cacgaagaat caagtgatct taaaaaaaac agtgttcatc
    18841 ggttcgctga atcagtcagt cgctcatcct agagaaattt tccattatgc tgtacgttat
    18901 tgtgcagcaa ggatcgtttt agcgcataat catccttctg gtaatgtttt aaccatagta
    18961 taataaataa tagtagttaa agtagtttaa gaggagatat taaagggata ttttacttta
    19021 aataaaaatt aacaaggttt ataatttgta gtagttaaag tatttaaaga ggtgggaaca
    19081 tgtgaaggtt caaaggatag aagtggagaa taagccgtat ccattgtatt tattactaga
    19141 taaagaatac cagctaatag aaccagtaat gaaatttatt aaatacttag ataatactgg
    19201 taagtctcct aataccatta aagcatactg ctatcattta aagttgctgt acgagttcat
    19261 ggaacagaga ggtgttattc ttaatgatat taactttgag ttgttagcag acttcgtagg
    19321 ttggttgaga tatccttcag catcaaatgt aattgatctt cagtcaaaaa aagccataag
    19381 agaagaaacg acagtgaata caattttaaa tgtagttatg agttttcttg attatttaag
    19441 tagattagga gaatttaaat caattgatgt atttaaacaa gcaaagggaa gaaatttcaa
    19501 aggattttta catcatgtta ataagggtag ataccaaaag aatgtcttaa agttaagggt
    19561 taaaaagaaa cagataagaa cattgagatc agaggaagtt aagcaaatta ttgatgcttg
    19621 tcatacgaaa agagataaat taattttaat gcttatgtat gagggtggtt taagaatcgg
    19681 tgaagtgtta tcgcttaggc ttgaagatat tgtcacttgg gacaatcaaa tccatttaac
    19741 acctagagat gttaatgtta atgaagctta tattaaatta aggaaggaaa gaacaataca
    19801 tgtgagtaaa gaacttatgt cactttatac agattacttg atatatgagt atagtgagga
    19861 attggagcat gattatgttt ttatttcctt aaaagaaggc tattttggga aaccactaaa
    19921 gtaccaaagt gttcttgatc tagttagaag aatagttaaa aggactggaa tagaatttac
    19981 atcacatatg cttcgccaca ctcacgcaac gcagctaatt agggaaggat gggatgttgc
    20041 gttcgttcaa aagagattag gtcacgcaca tgttcagaca acgttaaata cctatgttca
    20101 tctttcagat caggatatga aaaatgagtt taataaatac ctcgagagaa aggagcataa
    20161 gaaatgaatg cttctagtaa aaggaaaatt attagtcaga gtgagattag caaaaaaata
    20221 gctgtaatga atgaagaaat gcaggggttt tgggctaata atagttggga tataagaaaa
    20281 tgtccacatc cttctgccat agaattaagt aagaatcctg ctttaaggaa tcgttgggtt
    20341 cgttttgaac gtgttaaaaa tctgtggtta agaacagaat tgaaatattt ttatttttac
    20401 catttaaaca atggaatatg gaatgcaaaa actgtctgga ttagaaaagg aacagtaatt
    20461 aataaaatgt tagatttctt agatttaaag tatcctagca ttacttcaat tactgaagtt
    20521 cctattgaaa aagcaatgac ggagtataga acttatttga caaaacgggg tgttagaatt
    20581 accactacta attataagat tactgctaat caagaaaaaa cacctgtaaa agctaattcc
    20641 tactatgtta ctaatctaaa acaatttatg gagttttatg agaactttta ttttgatgga
    20701 gaggagtggg ataaagacgt ttgggataga cgtaacttac ctttgccaga tgataaggtt
    20761 aacccaacac aatatgaata tacaattaac tttaaagggt ttcggaatac atattttaaa
    20821 caacttgtaa aaagatattg taagttgaga ttgaacatgg atagcttttc ctatgtaagt
    20881 gatattgccc aaagacttaa agagttcttt aattttctgg acatgaaatt taaacaagtt
    20941 cagagagtac accaattaac gagagtggaa attgaagcat atttaagtga actaaacatg
    21001 atgggaataa aacctagtac aataactggg aggatctcta tattggaagg actatttagt
    21061 acccttctta ggctagaatg ggatgatgtt ccttccaaaa tattaattta ttctgaggac
    21121 tatccgaaaa taccaagagc aaaaccacgc tttatagatg aattcgtcct agagcaattg
    21181 aacagtcatc ttgataaatt acccgaatat atagctacga tgactatgat tgttcaagaa
    21241 tgtggaatga ggataagtga attgtgcacc ttgaaaaaag gctgtctatt agaggacaaa
    21301 gatggagatt tctttttaaa gtattatcaa tggaaaatga aaaaggagca tatagttcca
    21361 atatctaaag aggtagcttt acttattaaa gttcgggaag ataaagtttc agaggaattt
    21421 ccagatagtg aatacctctt tccaagaaaa gatggatcgc cattaaaaca agaaacattt
    21481 agaggtgagt taaataaatt agcttatgag caaaatatag tggataaatc aggtgagatt
    21541 tatagattcc atgcccatgc ctttcgccat acagtaggaa caagaatgat taacaacggg
    21601 atgccccagc atattgtgca gaaatttttg gggcatgaaa gcccagaaat gacaagcaga
    21661 tacgctcata tctttgatga aactctaaaa aatgaattta ctaaatttca ggaaaaactg
    21721 gttaccaata atggagatgt gcttgatcta gatgaagata atgaagtcga tgatgtagag
    21781 cttcaatggt tcaagaaaaa tataaatgca caagtgcttc caaatggtta ttgtagattg
    21841 ccagtagtag caggtggttg tccacatgcg aatgcatgct tagattgcac tcacttctgt
    21901 accagtaagc aattcttacc acagcacgaa gaacagttag agcgtacaga agagttatta
    21961 gccatagcta aggataaaca atggcaaaga caagtagaga ctaatagccg tgttaaagag
    22021 cgtttagaac aaatcattgg aagtttgacg gggtaattat caatggataa acaagttaga
    22081 aatacaacag aaattgtacg tttggcgaag cagaaatcaa aaaagacaag ggaaaaagta
    22141 gacaaagcga tttctaaatt ttcgattgaa ggtaaagtta ttaattttaa ttcaatagca
    22201 aaggaagcta atgtttctaa atcatggctt tataaggaac acgatattag gcaaagaatc
    22261 gaatcccttc gtgagcgtca aataacagca aatgtagtct caaaacccaa gaaaagttct
    22321 cgttcggagg aaatccttat taaaacctta aaaagaagag taatggaatt agaaaaagaa
    22381 aataaaaaat tacagaacca aattcaaaaa ttatatggag atctgtataa taaagaataa
    22441 ttattaatct gtagacaaat tgtgaaagga tgtacttaaa cgctaacggt cagctttatt
    22501 gaacagtcat ttaggtatat gtccaatcta gggtaagtaa attgagtatc aatataaact
    22561 ttatatgaac ataatcagtt acgaggtgaa atcatgagca atttgattaa cggaaaaata
    22621 ccaaatcaag cgattcaaac attaaaaatc gtaaaagatt tatttggaag ttcaatagtt
    22681 ggagtatatc tatttggttc agcagtaaat ggtggtttac gcattaacag cgatgtagat
    22741 gttctagtcg tcgtgaatca tagtttacct caattaactc gaaaaaaact aacagaaaga
    22801 ctaatgacta tatcaggaaa gattggaaat acggattctg ttagaccact tgaagttacg
    22861 gttataaata ggagtgaagt tgtcccttgg caatatcctc caaaaagaga atttatatac
    22921 ggtgagtggc tcaggggtga atttgagaat ggacaaattc aggaaccaag ctatgatcct
    22981 gatttggcta ttgttttagc acaagcaaga aagaatagta tttctctatt tggtcctgat
    23041 tcttcaagta tacttgtctc cgtacctttg acagatattc gaagagcaat taaggattct
    23101 ttgccagaac taattgaggg gataaaaggt gatgagcgta atgtaatttt aaccctagct
    23161 cgaatgtggc aaacagtgac tactggtgaa attacctcga aagatgtcgc tgcagaatgg
    23221 gctatacctc ttttacctaa agagcatgta actttactgg atatagctag aaaaggctat
    23281 cggggagagt gtgatgataa gtgggaagga ctatattcaa aggtgaaagc actcgttaag
    23341 tatatgaaaa attctataga aacttctctc aattaggcta attttattgc aataacaggt
    23401 gcttactttt aaaactactg atttattgat aaatattgaa caatttttgg gaagaataaa
    23461 gcgtcctctt gtgaaattag agaacgcttt attactttaa tttagtgaaa caatttgtaa
    23521 ctattgaaaa tagaaagaaa ttgttccttc gatagtttat taatattagt gacatttgca
    23581 tgcttcaaag cctgtcggaa ttggttttta gtgaaaagaa cacgatattc acggtttacc
    23641 cacttataaa caaaagatcg atactttttg tagtccttct ttgaaatcaa tggttgatgt
    23701 cgttcaagaa caatcaatac agagtctaca cttggcttag gatgaaaata tagtggtggt
    23761 acttttttga gcatttttat atccatctcc accattaata gtaaacccaa agctcgttgc
    23821 agattttgca atcttttcgc aaatcccttc tcaacgataa gatagctata tttagcctga
    23881 ctttcaaagg taattctttt gacaatatcc gtactgatgt tataaggaat attaccatat
    23941 atcttatagt ttatatgttt tgggaaggaa aattttagaa tatccgtttg aatcactttt
    24001 atattctcag aggggtttac cgcttcttta gtcacttgac ataagcctcc atcaatttct
    24061 atagcagtaa ctgatcgact cattttgact agctctttgg taaaatgtcc ttttcctgat
    24121 ccgatttcta ttacgttgtc ttgtttactg atattcgtgt gattcaatat ttcttttaca
    24181 tgctttttag aagtaataaa attttgcgtg tctttagggt ttttctggtt cattataacc
    24241 ttctccttac tggttataat gaactgacat taagcgttca ttataaccaa ttaattttga
    24301 tttggttgat aacgaacttt attaataaca aatatagaaa aagtacccat acttttatct
    24361 cctttccatt tttttcatta tgaatgagat aaagtaattt ctactactgc gatactggtg
    24421 cacataattg aaacctcctt atgtaactga atatgattgt atcatctttt cattttagaa
    24481 taaaccttta tatgtaattt tgcacttgac acttcagatg gacgttttct tcaaaataat
    24541 atgttttgaa acttgataag agaagccact gtctttacta aaaagtaaag gctgcgataa
    24601 aggacagtga ctttaataaa gttgcactgt tgtattgtct ttggataaaa ttgtctaaag
    24661 ggaagtttta tccccaacga aaaaacatct aaagtggtaa gtaatgaaag aatgtcgata
    24721 aagtaggaat gggggatctt tttgaataca atagaatcac agtttgataa ggttgcagaa
    24781 gattacgatt tcgtgaatga gcttttgaat gattattcat tctttgtgtc taatatgtct
    24841 ccaaagaaag gcagagcatt agatatcgga tgtggctcgg gtttgttagt ggagaaatta
    24901 gcaagttatt atgatgaagt ggtagggatt gatatttcta atcaaatgct cgatcttgcc
    24961 aaatctaaac gtcaactaac aaatacggtc tatctgaata tgaatgcaga acaacttaat
    25021 tttaatgaga agtttgattt tattgtaagc cgaacaacct ttcatcattt ggatgatata
    25081 gccagcgtaa tacagcaaat gaaggaactg ttgaatgagg aaggaagaat agttattctt
    25141 gataatgtat ctgaagtcga gacaccgcct acctatgttt ataaattagg ggcaatccaa
    25201 gagtttttac cgcattgttt caaatttgga ataaaaaatg cgattagaat ttataatcac
    25261 aacacatcta aatcatggct tgagcattta gcttcagata agtacctatc tgaacaaaat
    25321 tattacgatt tatatgaaaa gttgttgcct ggatgccaat tccataaaat gggttgggca
    25381 atgggggttg tctggacaaa ataacttaaa gctgtggctt tgaaaagttg aatttgtgat
    25441 tgttttcaga gctatcgggt gctttagtat aaataacaat gaataaaaaa tcagcctgta
    25501 tcttatgtta ctattaagta atttaagata caggttttac tatttttgaa ataagtacct
    25561 tccacttcaa atacatgtat tcaaagtgaa agtattgatt taaatgattc ttttgtaatt
    25621 tactcttgat aatatgaaat tccatcgcag caggatatga attttaccaa aaggatacaa
    25681 aagtgtggag aaatgatggg gatcactgta ctggaccact tgatcatcgg acgcaaacga
    25741 tattttagtt tgagagaaga aggaatgatg gaagagaagt aagttgcttt cttgcaaaaa
    25801 aaatgaagca agtgtacaat tatattgtaa ttttgtttgg gaatagaagt tgaaacatct
    25861 gttgcacctc tatcgtacac gagcaatgtt acaatttaaa ttagtgcttt tggcacgagg
    25921 aggatattat tatggaaaac ggaacagtaa aatggttcaa cgctgaaaaa ggatttggat
    25981 ttatctcacg tgaagatgga agcgacgtat tcgttcactt ctcagctatc caaggtgacg
    26041 gattcaaaac attagaagaa ggtcaagcag tgactttcga cgttgaagaa tcagaccgcg
    26101 gcccacaagc tgcaaacgtt gtaaaagctt aattttatct gtaaatcaaa ttgatggtag
    26161 acatcaattt ttacgagaga acaaagcgag taacaaaaat acctgccaca agagacaatt
    26221 tctcttgtgg caggtatttt tttgtatgaa aaagtaaata aatttaagag gctatgaaag
    26281 agccaccttg tatcaaggaa taagaacaaa caaaaatggc ttctcatgtt tttcatttgt
    26341 tttctcttat attcaggaca aggtgctgat ttcataacct cttatttatt cttcatctgg
    26401 tgtcattacc attggcaaaa tcattggatg tctttctgta cgttcgaata agaaaggctg
    26461 aagcgcgcca gtcatcgctt ctttcaattt atattctgaa cagtcaggtc cattcaaaca
    26521 ttctcttaaa gcgtggaata agattcgctg agcttcattg atcatttctc ctgattctcg
    26581 catgtagaca aatccacgag agagaatatc tggaccagcc aagatttctt ttttcttgat
    26641 atccactgtc gcgacagcta atacaagacc ttcttctgaa agcaggtgac ggtcacgtaa
    26701 aacgacatta ccaatatcac cgatcccgct accatcgata tacacatctc cagccacaaa
    26761 atgcccagca ggacgcgcag aatcgctagt taacgctaaa acatcgccat tttccatgat
    26821 gaagcagttt tctgcaggaa cacctacatc ttgtgctaga gaagcatgta ctttcaacat
    26881 gcggaattct ccgtggacag gaataaaata ttttggcttc atcaaacgaa gcattaattt
    26941 ttgttcttct tgtccaccgt gaccggaagt atggatgtta ttgatttttc cgtgaatcac
    27001 ttctgcacca gcttcagaca gcaaattgat caaatgattg acacttgttg tattaccagg
    27061 aataggtgaa cttgaaaaga tcactgtatc gtctggctgg atttggattt gacggtgagt
    27121 accattcgcg atgcggctca acgcggccat cgcttctcct tgagaacctg tacataggat
    27181 catcgtttca ttggcaggca agcgattgat ttcgtgtgca tctacaaatg tgccgtctgg
    27241 aactttgata tatcctaaac gttgaccgtt gacgatggca ttttccatgc ttcgtccgaa
    27301 aacagcgatt ttacgaccag ttgccacagc ggcatctgct gcttgctgca aacggaagat
    27361 gttagaagca aagctggcaa aaatgatacg tccttcgatt tttgaaaaga tcttcaaaat
    27421 agaatgccca atcgtttttt ctgatttagt aaatgttgga atctcagcat ttgtactatc
    27481 agataaaagg cacaagactc cttcttcccc gatacgagcc attttatgca agtttgctgg
    27541 ttcgccaaca ggagtgaaat cgaatttgaa gtctccagtc gctacgatat ttccagaagg
    27601 tgttttcacc acgatcccta atgcatctgg gatactatgg gtcgttctga aaaagctgac
    27661 agaagtcttt ctaaaacgga tgatcgtatc ttcattgatc tcatggagct ctgcatctcg
    27721 aagcaagcca tgctcatcta atttgtttgt gatcaacgct aatgcgagag gaccagcata
    27781 gataggaata tttgcttgtt tcagcaagta gggtattccg ccgatgtggt cttcgtgccc
    27841 gtgagtaatc accaaggctt ttactttgtg taagttttgt atgatgtagc tgtagtcggg
    27901 aatgacatag tcaattccaa gtaaatcgtc ttccgggaat ttgatcccgg catcaatcaa
    27961 aatgatttca tcttggaatt gtaccccata cgtatttttc ccgatttcac ctaatccacc
    28021 aatagcaaag acgcctgtct cgttattttt gatggaaagt ttcatattaa aactccacta
    28081 atgagaaatc agcattttct ttttcgtact ctagatgact tccttctaga agttgaacga
    28141 attcaatatt gtatggggta ttttcttcca cacgttggcg agcgtctaca tcgctttttg
    28201 cctcaatgta aagagatttt gtttcttccc gtttagggtt gcgtactttt gtttcttgat
    28261 aataaacttt atagatcatt gttttgtctc ctttatctgc gtaaagcagt gatttagcta
    28321 gttgattttt ttagacaaca caaaacgagg ggacaagcca cctggttttt catatgcttt
    28381 gactagaata ctggattcga gtcaaagaga caagcctcgt ttttgcttat ctgttcggat
    28441 attttttcaa ccgtaccttc tgtataaaaa atatacaaca attaattgtt attctagcat
    28501 aaaacagcaa ataagtatag aaatcaacta gattgaaaga aaaaatcaat gtttatcatc
    28561 ccgctataat cataatgact tttttatgag agaagtgttc tattaatagg ctcgttgtgc
    28621 tatcattttt agtaatgaaa atgtaacaaa agattaaaag gagatgagaa agtgaaacta
    28681 atgtggcatt ataccatgcg ttataagaaa ttcttgtttt tcaatttcat atgcgtattt
    28741 ggttttattt tgattgaatt aggacttcct acgattttag cacgtatgat cgatgtagga
    28801 atccgcaatg atgattttga ttatgtgaag caacaaggga tatttatgat cgttattact
    28861 gtaatcggta ttattttgaa tatcttgtta ggttattttg gttcgaggat tacgactaat
    28921 atcgtagcag atattcgaga tgatttgttc gaaaagatcc aaagttattc gcatcaggaa
    28981 tacgaaaatc taggcgtatc ttcattgatc actcgtacaa caaacgatgc ttatcagatc
    29041 atgttgttcc tgcaaaatat tttacgtatc ggttttatgg caccaatgat gttcattgtc
    29101 agtttgtaca tggtcatgcg tacaagtgtg acactcagtt tgtatgtagt aggtgcatta
    29161 cctttactat tattggcagt cgtagcgatt gctaaggtgt ctgaaccgtt atctaaaaag
    29221 caacaaaaaa acttagataa aatcaacagt atcttgcggg aaaatctttc aggattgcgt
    29281 gtgattcgtg cattcgtcaa tgaaaagttt gaagaaaaac gttttgctgt agtcaatgag
    29341 aattatgctt ctagttcaaa aagcttattc cgtttgatgg ctgttgcaca accgggtttt
    29401 tatttcttat ttaatatcgt aatggtattg atcatctgga gcggggcagt gcaaatcgat
    29461 gcaggcgagc ttcgtgtagg agatttgatt gcgtttatcg agtatatatt ccatgccttg
    29521 ttctcattca tgttatttgc tagtgtgttc atgatgtatc caagagcagc tgtttcagct
    29581 ggacgtatcc aagaagccct agatgccaaa ccttcgattg tagaagatcc aaatggtata
    29641 tctgatacaa aaactaaagg gtatatcgaa tttcaaaatg taacatttgc ttatccaggc
    29701 cattcgcaag agccagtgat acgaaatgtc agttttacag cctctccagg agagaccgtt
    29761 gcttttattg gaagtaccgg tagcggaaaa tctacgttga tccaattgat ccctcgcttt
    29821 tatgatgtga gtgaaggaaa gatcttgatc gatggagtag acgtgagaga ttatcggcta
    29881 agcaagttgc gagataagat tgggtttatt ccgcaaaaag cgctgctatt tactggaacg
    29941 attgcagata atttgcgata cgggaaagaa gatgctacgc aagaagaaat ggaacgtgca
    30001 gctgagattg ctcaagcttc agaattcatt tctcgaaaac cagatggatt taatgaacat
    30061 ttatcagaag gtggttccaa tttttcaggt ggacaaaaac aacgattggc aatagccaga
    30121 gccatcatac gccgtccgga aatctatatt tttgatgaca gcttttctgc attagattat
    30181 caaacggatg cgaagttacg tgctcgtctg aaaaaagaaa cgacagaatc tactgtattg
    30241 atcgtagcac aacgtgtggg aacgatcatg catgcagata agatcatcgt attaaatgaa
    30301 ggagaagtcg tcggtatggg gacgcatcgg gagttgttgg aaaaatgccc gatttattat
    30361 gatattgccg cttctcaatt atcaaaggag gaattagcat gaaaaaaacc ttttcttcct
    30421 taaaacgttt agctcgatat atcagaccgt atcgtgagac ttttctttta gtgatcctat
    30481 ttacagttct gacagtggct ttcaacgtgg ctttgccata tgttacagga cttcctacta
    30541 cagaaatcag ccgtaatatt gccaatggcg aatcaatcaa tttttcttat atattcaaat
    30601 gtctcatttg gatcacagtt gttggaatag ggtattgtat ttcgcaatta ctttctggtg
    30661 ttttgatgac aaatgtcgtt caaagtgcca tgcatgattt gagaagagac atagaagaaa
    30721 aaatcaatcg tcttccggtt tcatatttcg ataaaaacca gcaaggaaat attttgtctc
    30781 gtgtgacgaa tgatgtagat gcagtcagcg gtgctttaca acaagctttt attggtatcg
    30841 taaatgcgat attgggaatt acgttggctg cagcaatgat gttttatatc cagccgatga
    30901 tggcgctgat ctctatgatc atgattccaa catcgatctg gatctcaaaa cgagtgatca
    30961 atgcttcgca gaaatatttt caaagcatgc agaattctct tggtgagcta aacggctacg
    31021 tccaagaaaa tatgacaggt tttagtgtat tgaaagtata tggacgcgaa aaagaaacat
    31081 tggaaggatt caaaaaagtt aatcacagct tgaaatactt tgggtttcgt gcagcattca
    31141 tttctggctt gatgatgcct ctagttcaat taactgccta tgctacttat attggtatgg
    31201 ctgttctagg aagcttttat gttattactg gggcgatcgt tgtcggtcaa ctacaagcat
    31261 tcatccaata tatttggcaa atcagtcagc caatggggaa cgtgacacaa ttgtcttcag
    31321 ttttgcaaag tgcatcagcg gcaacgaaac gtgtattcga gattttagat gagccagaag
    31381 aaaaagaaaa caaagtagat gcttcactgc cagaaacgat tttaggagat gtgacattcg
    31441 atcatgtcga ttttgcatat gatccgaaga aaccattaat ccaagatttg aattttgaag
    31501 tgaaagctgg tcaaaccgta gctgtggtcg gtccgaccgg tgccggcaag acgacattga
    31561 tcaatctatt gatgcgtttt tatgacgtag acaaaggagc aataaggatt gatggaatcg
    31621 acaccaaatc aatgagtcgc agtgatgtgc gttcgctatt tggtatggtc ttgcaagatg
    31681 catggcttta tcaaggaacg attgcagata atatccgctt cggaaagctt gatgcgacag
    31741 attatgaagt agttgatgct gcgaaaacag caaacgtaga tcattttatc cgaacgatgc
    31801 cagatggata tgatatggcc atcaattctg agggagacaa tgtgtcttta ggacaaaaac
    31861 agttgctgac gattgcccga gcagtggtct ccgatcctaa aatcttgatt ttagatgaag
    31921 caacaagttc agtagacacc cgtttagaag cgttgattca aaaagcaatg gatcgcgtga
    31981 tggagggacg gacaagtttc gtcatcgctc accgtttatc gacaatccgt gatgcagatc
    32041 tgattctagt gatggatcaa ggtcagatca ttgaaaaagg aacgcatgag agtttattgg
    32101 cacaaggtgg attttatgaa aaactgtata acagccaatt tgctgaggaa gcagaataag
    32161 tcatataaga ataaaacagt ttaaaactta ttttttgttc tctattcttg ataatggtaa
    32221 agtaaagtta tcaagaaaag tgaggacacg gagatggaac agtttttaaa tgaaaaaatt
    32281 actgaagcca aaagagaagt tgatcgttta acgcacgtct gtcagacgaa gccttttgat
    32341 tcagctgatt tttttgaaaa tgagtttcaa atcgaacgct tggcagcaca aatagaagtg
    32401 tacgaattaa ttttaaaaaa agtaatagaa gaaaatatag atgtacaata aaaacaggta
    32461 aaacgtcagt taccagatac tgtaaataga catctggcct gagcaggttt tacctgtttt
    32521 ttttcttatg aaataaatat atgctatgat acctttattc aataggatta gtttctacaa
    32581 aaaatgaaag gagaaaaata aatatggaac ggtttatagc atcaaatgcc acagtgattg
    32641 gtgatgtgac attgtcagaa gatgtgacga tctggtatca agctgtttta cgcggggaca
    32701 gtaattggat aaaaatagga caaagaacga atatccaaga tggtacaatc atccatgtcg
    32761 atcatgatgc acctgtagat attgcggaga atgtgacggt aggacatcag tgcatgctac
    32821 atggctgtac aatcgaaaaa ggggcgctga tcgggatggg gaccacgata ttgaatcatg
    32881 cggttattgg tgagaatagt ttgattggtg ctggatcatt agtaacagaa ggaaaagtta
    32941 ttcctcctaa tgtcttggct tttggtcgcc cagctaaagt gatccgtcct ttgacaaaag
    33001 aagaaataca aaaaaacaaa gaaaacatcc agcattatgt cgagataggt cggatgcatg
    33061 cgcaaggaaa attcaaggag caaagatagg cagtagcatc cataaaataa aaaaggatgg
    33121 tcagtttgag ttgaccatcc ttttttattt ggtttagata agagagctta aaaaagcttc
    33181 ctttgaaaca gattaattta atgaagaact gttttccatg acttcatcga tcaatccata
    33241 tgcttttgct tgttcagctg tcatatagtt gtcacgatct gtatcttttt caatcacttc
    33301 aagcggttgt ccagtgcgtt cagctaaaat tttgtttaag cgttcgcgtg tttgcaaaat
    33361 atggcgtgcc gcaatttcga tttcagttgc ttgcccttga gcgccgccaa gaggttgatg
    33421 gatcatgatc tcagcatttg gtaaagcaaa tcgtttacct tttgtaccag ctgtcagcaa
    33481 gaagcttccc attgaagcag ccatacccat aacaattgtt tggacatctg ctttgacaaa
    33541 gttcattgtg tcgtaaatag ccatacctgc tgtaactgaa ccaccaggag aattgatata
    33601 aagatagata tctttttcag aatcttgagc gtctaagaaa agcaattggg cgatgatcga
    33661 attagcaaga tcatctgtta cttggccgct taacatgatg atacggtctt tcaataaacg
    33721 tgagtagata tcgtaagctc tttcacctcg tgaagattgt tcaatgactg taggaattaa
    33781 attcataata gagttcctcc ttgatttcgt ttagaaatag tgtaccataa tttagtttct
    33841 aatacctaaa atgtagtata catttttggt caaaaaaggt caaataaaaa acttaggcaa
    33901 attagtcgca taaacccgct ttatggtatg ataagcagag aaacaaaaca aggaggaaca
    33961 acagattgat agttggtatt actggcctta tcggagtgct gacaggtgct gtagttgtct
    34021 atatcggcat gatactgcaa atgcgtcact tggaaaataa agaacagcgt agacgtgaat
    34081 tggaattgaa agtcaaagaa atcgagacgc tgaatcaatt aaataaaaag atcaacgaga
    34141 tcttgcaaaa acggacaacg ctgatggaag aatatgtcag ctttgatgca tttgatgatt
    34201 gttatatcac gattgacgat tttgcgtatc tccaatcatt ttcagcgcaa aatagttttt
    34261 atctgccaaa ttatatttta gacgagctat ttaagaatat ctcccatcga aaagtgattt
    34321 tatctccaga tgaaacaatg aagattggtg gatatgctta taaaggcgga cgcgtgattt
    34381 tagagaattt tacggatagt ctgactgaaa tgatcaatga aaaaaaagct cagttgaaga
    34441 ctatgacgcg ccagccgatt ggtttcttct cgaaagatga tgtttggagc tgatattttg
    34501 aagcattgcc gttgcacatg agagcgtggt aatgctttct ttttttctta atgatacaat
    34561 agaactgaac ggagtgaaga aagatgttac aaatctatgc ccatcgcgga agcagcggca
    34621 cccacccaga aaatacgctt cctgcatttg cagaagctgt acgagtaggt gcagatggta
    34681 tcgaactaga tgtccatcta agcaaagatg gttatttgat cgttatgcac gacgaagaag
    34741 tggaccggac gactaacggt aaagggctga ttcgagaaaa aacattggaa gaactcaaaa
    34801 aattgaatgc agggggcaaa ttcagcaaag aatttccagc agctaaagtt cctgaattga
    34861 gagaagtgtt aggactgctc ctccaaaaaa attaccgagg gatgctaatg attgaaatta
    34921 aaactgatca gtatgaatat cccggtatcg aagaaaaatt agccgaaaca ttaaaaagta
    34981 gagattggcc ttttcaacat gcatattgca gctttcattt ggattctttg gaaaaaatcg
    35041 cattactgga accggagata cagctggact tattgatgag tacttctgat aaaaagatcc
    35101 aactagcagc agaaacccct tatatagaag gagtacatcc aaaaaatact tggttgttcg
    35161 aacatccagt ccaggcagct gattttccaa aagcaattcg tgcatggacg attaacgatg
    35221 aagaagaaat ggccaaatgt caagaactca aaatagatgg aataataact gattttccgg
    35281 agaaagctct tcggtggaaa caatcaacga aaagtaggta aaatatggaa aaagtattag
    35341 tgatcgtcgg tccgacagca gtcggtaaaa cagcgctaag tgtggaactc gcacaactat
    35401 ttggaggaga gatcatcagt ggtgattcat tgcaaatcta taaaaatctg gatatcggta
    35461 cagcaaaaat caaagaagaa gagatgaata atgttccgca tcatttgatt gatgtgatcg
    35521 aacctactca acgttactca gccgctgatt ttcagcattc aggtcgacaa ttgattaccg
    35581 agattgcgaa ccgagggcac ctacctatca tagctggtgg tacgggtctc tatatccaat
    35641 cccttctata tgattatcag ttaggagcga cagaagaagc gaatcctgag attagaaaaa
    35701 agtatgaaac gttagctgaa acaattggtt cccaagcatt atggaatcag ttgaaagaaa
    35761 aagacccggc agctgctgag aaaatccact ggaacaatca acgcaaagtg ataagagctc
    35821 tggaagtttt agagacgaca ggacatagta tcacggcacc taaagaagag ccaaagcaat
    35881 tgtatgatta cctcatgatt ggattagata cgaagcgtag tattctttat gaacgaatca
    35941 atcaaagagt cgatttgatg ctagcagaag gtttagtagc agaagcgcgt aaggtgtatg
    36001 agcttggaga agtccaggcc agtcaaggta ttggatataa agaatttttc ccttattttg
    36061 aagggaaaga aacattggaa gaagcaacag aacaagtcaa actacattcg agaagatacg
    36121 cgaaacgtca gttgacgtgg tttcgtaacc gtttggatgc acagtggttt gatcttataa
    36181 gtgagccaaa tcaaaaaagt cagattgagc aaacaatcaa aaaatggctg gaggcggaat
    36241 aaaatggaag aaaaagtgat catcgtaggg gtagaaaccg aagcaaacca gcgttacttt
    36301 tcagagtcaa tggaagagct agttcagctg acgaataccg catcaggaga agtcgtattc
    36361 acaattacac aaaagagacc acaagtggat cggcaaacga ttatcggtaa agggaaatta
    36421 caagaattag ttcaacaagc ggatgcgcat gaagcggatt tgatcatctt taatcatgag
    36481 ctaactccga gacagagcca actgattacc gatgctgttg gtcttccggt aattgatcgc
    36541 gtgcagctta tcttagatat ttttgcgatg cgtgctcgtt cgaaagaagg gaagctgcag
    36601 gttgaacttg ctcagttaga atacctgctg cctcgtttag tagggcaagg gaaaacatta
    36661 tctcgtcttg gcggtgggat cggaacaaga gggccaggtg aaacaaagtt agaaacagac
    36721 cgccgacata tacggaataa aatttttgct gttaagaaag aattgaaaga agtagaagct
    36781 catcgagagc gcaatcgcca aaaaagaaaa aatagcgaga tttttcagat tggacttatt
    36841 ggctacacga atgccgggaa gtcaactatc ttgaatctat tgacacaagc ggatacttat
    36901 tcgaaagatc aattgtttgc aacgttagat ccattgacta agagatggcg ttttgctgaa
    36961 ggatttgaga ttactgtaac ggataccgtc gggttcatcc aagacctgcc tacgcaactg
    37021 atcgatgctt tccattctac attagaagaa agtcaaaata tggatctttt gctacatgta
    37081 gtcgatgcta gttctccaga tcgtatttta caggagcaga ctgtcttaaa attgatggat
    37141 gaactgaata tgaaagaaat gccgatactg actgtttaca ataaggcaga ccagatcgac
    37201 ccagcgatgt tcacgcctac gctattccca aatgtattga tttctactca gacgaaagaa
    37261 ggtaagatgg cgttgatcga agcaatcaaa cgtcagctga tggaactaat gaccccttat
    37321 acactttctg tgccaagcaa cgaaggacag aaattaagcg agttgagaag acaaacaatg
    37381 gttttggacg aagcgtacat ggaagaaaca aacgaatatg aagttaaagg gtttgcaaaa
    37441 accaattcaa aatggttaag aaaaaaccaa taaaatttta tttttttaa
//
LOCUS       DACDKJ010000021        35903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDKJ010000021 DACDKJ010000000
VERSION     DACDKJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.021"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..884
                     /locus_tag="IV328_001206"
     CDS             <1..884
                     /locus_tag="IV328_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8937360.1"
                     /translation="DDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAG
                     IPNIAIVTNGSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAI
                     KLFRELSEEVDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVP
                     TVSQYRKLVSDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMAS
                     PYLPIQVGNIKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLN
                     EDIEIDILDEYKHDILI"
     gene            940..1158
                     /locus_tag="IV328_001207"
     CDS             940..1158
                     /locus_tag="IV328_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937361.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1229..2044
                     /locus_tag="IV328_001208"
     CDS             1229..2044
                     /locus_tag="IV328_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937362.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            2108..2443
                     /locus_tag="IV328_001209"
     CDS             2108..2443
                     /locus_tag="IV328_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8937363.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCL
                     IVHEVNKQL"
     gene            2867..3130
                     /locus_tag="IV328_001210"
     CDS             2867..3130
                     /locus_tag="IV328_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937364.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            3446..3876
                     /locus_tag="IV328_001211"
                     /pseudo
     CDS             3446..3876
                     /locus_tag="IV328_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            4015..4437
                     /locus_tag="IV328_001212"
     CDS             4015..4437
                     /locus_tag="IV328_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937365.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            4582..4827
                     /locus_tag="IV328_001213"
                     /pseudo
     CDS             4582..4827
                     /locus_tag="IV328_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4936..5892
                     /locus_tag="IV328_001214"
     CDS             4936..5892
                     /locus_tag="IV328_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937366.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(6728..7399)
                     /locus_tag="IV328_001215"
     CDS             complement(6728..7399)
                     /locus_tag="IV328_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8937367.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            7716..9347
                     /locus_tag="IV328_001216"
     CDS             7716..9347
                     /locus_tag="IV328_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8937368.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            9350..10429
                     /gene="uxuA"
                     /locus_tag="IV328_001217"
     CDS             9350..10429
                     /gene="uxuA"
                     /locus_tag="IV328_001217"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8937369.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            10503..11930
                     /gene="uxaC"
                     /locus_tag="IV328_001218"
     CDS             10503..11930
                     /gene="uxaC"
                     /locus_tag="IV328_001218"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8937370.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            12014..12661
                     /locus_tag="IV328_001219"
     CDS             12014..12661
                     /locus_tag="IV328_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8937371.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            12790..14166
                     /locus_tag="IV328_001220"
     CDS             12790..14166
                     /locus_tag="IV328_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8937372.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            14177..14971
                     /locus_tag="IV328_001221"
     CDS             14177..14971
                     /locus_tag="IV328_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8937373.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            14984..16750
                     /locus_tag="IV328_001222"
     CDS             14984..16750
                     /locus_tag="IV328_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8937374.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            16778..18586
                     /gene="uidA"
                     /locus_tag="IV328_001223"
     CDS             16778..18586
                     /gene="uidA"
                     /locus_tag="IV328_001223"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8937375.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            18758..19159
                     /locus_tag="IV328_001224"
     CDS             18758..19159
                     /locus_tag="IV328_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8937376.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            19153..19398
                     /locus_tag="IV328_001225"
     CDS             19153..19398
                     /locus_tag="IV328_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8937377.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(19722..19943)
                     /locus_tag="IV328_001226"
     CDS             complement(19722..19943)
                     /locus_tag="IV328_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937378.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(20105..20177)
                     /locus_tag="IV328_001227"
     tRNA            complement(20105..20177)
                     /locus_tag="IV328_001227"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20142..20144),aa:Lys,seq:ctt)
     gene            20253..20483
                     /locus_tag="IV328_001228"
     CDS             20253..20483
                     /locus_tag="IV328_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8937379.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            20527..21696
                     /locus_tag="IV328_001229"
     CDS             20527..21696
                     /locus_tag="IV328_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8937380.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            22270..22839
                     /locus_tag="IV328_001230"
     CDS             22270..22839
                     /locus_tag="IV328_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8937381.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            22826..23686
                     /locus_tag="IV328_001231"
     CDS             22826..23686
                     /locus_tag="IV328_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8937382.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            23876..24580
                     /locus_tag="IV328_001232"
     CDS             23876..24580
                     /locus_tag="IV328_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8937383.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            24585..26420
                     /gene="walK"
                     /locus_tag="IV328_001233"
     CDS             24585..26420
                     /gene="walK"
                     /locus_tag="IV328_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8937384.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            26417..27733
                     /locus_tag="IV328_001234"
     CDS             26417..27733
                     /locus_tag="IV328_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937385.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            27734..28603
                     /locus_tag="IV328_001235"
     CDS             27734..28603
                     /locus_tag="IV328_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937386.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            28658..29467
                     /locus_tag="IV328_001236"
     CDS             28658..29467
                     /locus_tag="IV328_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8937387.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(29570..30199)
                     /locus_tag="IV328_001237"
     CDS             complement(29570..30199)
                     /locus_tag="IV328_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP8937388.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            30369..31661
                     /locus_tag="IV328_001238"
     CDS             30369..31661
                     /locus_tag="IV328_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8937389.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(31737..32399)
                     /locus_tag="IV328_001239"
     CDS             complement(31737..32399)
                     /locus_tag="IV328_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8937390.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            32624..32908
                     /locus_tag="IV328_001240"
     CDS             32624..32908
                     /locus_tag="IV328_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8937391.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            32959..34584
                     /gene="groL"
                     /locus_tag="IV328_001241"
     CDS             32959..34584
                     /gene="groL"
                     /locus_tag="IV328_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8937392.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(34673..35821)
                     /locus_tag="IV328_001242"
     CDS             complement(34673..35821)
                     /locus_tag="IV328_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8937393.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
BASE COUNT        12386 a         5340 c         7188 g        10989 t
ORIGIN      
        1 ctgatgatga actgttagaa cttacaaaag atatatgtaa tttaaagcca tataatattt
       61 gtttctgtgg aggagaaact ttactgagaa aaaaaatagt tttatcttgt gccactttat
      121 taaaagaggc aggtattcct aatattgcta ttgttactaa tggatcatta gtaactcagg
      181 atgttgcaaa agaattattc aaagcaggta ttaatcgatt tcaagtaagt ttagatggaa
      241 aaaatgctag tagccatgaa gcacttagat tatatccaaa cagttttgaa aaggcaatta
      301 atgctatcaa attatttaga gaattgtccg aagaagtgga tgttgctttt tgtcctacta
      361 gttttaatta caatcaatta cgagaagtat atcaactagt aaatactctt aaagtgaagt
      421 ctttaagaat tcaaccattt atgcttagtg gtaggggaat gcaaaatagg gaaatagtgc
      481 caactgtttc tcaatataga aaactagttt ctgatattaa ctatattaaa agaaatgaag
      541 attataccaa gatagattgg ggagatccaa ttgatcatat catccgacac agaactttat
      601 tgaaagattt ttcgaatttt gtttctatta ggtcagacgg aggattaatg gcatcacctt
      661 acttgccaat tcaagttgga aatattaaat gtcacagtct ttctaaatat tggcgagcag
      721 gactttataa agttttttct ttagatttag ttcaagatat agctactcaa gtttcttcaa
      781 tacaagatat gagtaaagaa ttaaaaaaag gacatattaa atggcttaat gaagatatag
      841 aaattgatat tctcgatgaa tacaaacatg atattttaat ttaggtttat cattggaaag
      901 tttagataaa caaagtactt ttagaaagga gggaaaaaaa tgatttctga acctcaaatg
      961 aataatatgt atgaaagccc aactataatg ggagctggtg aagctgcgcc aagagcttgt
     1021 tcagcaatta tggtgtgtgt gttattagca tctgcaggag taggtatcca agcaggagcg
     1081 gtgtatactt atgctgtagt tgctacagta gctgtagctg cagccgcagt ttggaaaaca
     1141 gtaggattag ttggttagga attaatagga gactaactgt ctcctattga ttccaatgat
     1201 taagctaagg aggaatttta agaaaataat gaaatacaat aatttaaaac cgaaattact
     1261 taatgagaac atagaatata taagagaaga aaattatgga tatttaacca tagttcccaa
     1321 aaatggaact gaattgaaag ataacgtttt caatccaaca gtaagatata tattggctaa
     1381 ctgtactgga aaaaacagtg taaaaaaaat agcgaatggt ttaaaaaaga agtttcctag
     1441 tttagaatat gaaaaattct atcaagatgt tgtaggaact attaaatttt tcaaacacta
     1501 taatattatt gatataggag attatagtat ggaagaaaag aaaatatata aaattgaaca
     1561 aaatttagaa ttagaattac taaatgaatc acattatgaa gaaatttata attttatcac
     1621 agatcatcct gaaagtatgt attttcatga agatatttat catcaaggaa atagtaaaga
     1681 attagaagtt gtccttagat ataatttatt ttcgtttacg agtgaatttt actttttaag
     1741 acataaagag agaattgtag gaatggtttc tataattgtt aatccatttc aaaacttatc
     1801 ctacagtata gtaaattacc tcatatgtga gcctgcatat actacgacta ttttgacaac
     1861 agtattagaa aatattaacg aagtttcaat tttagaagta aagaagctag tgattcattt
     1921 gctaaattca acagaagatg aatcactaat cccctattta aaagattcac atttttttaa
     1981 agaagcggta ctatctaaag aatatagaga gcatgatgtt ttgagatacg cttacattta
     2041 ttaaataaaa tttctgatag tattttaata attcggatca tatcataaga tataggaggg
     2101 aaaagaagtg attttattga ttttgtctct cgtattacta gttgtatcta atctaatgaa
     2161 aaataatcct cctacaaaaa ttaattcttt ttatggcttt agaactaaaa agtcaatgaa
     2221 aaatgaaaaa agttggcatt tagcacaaaa attgttgggg gatttttcaa aaaaaaaagt
     2281 tatcccagta tttttgatgg gtattgtttt tttcagtata gagatttata gtttagctat
     2341 tttaaaaaat gaaaattttt tcgttagtgt attaatgggt gaaagtttaa tcctaatact
     2401 gattttgtgt ttaattgttc atgaagtgaa taaacaattg tgaattaaaa aaataaaaac
     2461 ctagtataat cactttaata aattgttggt agaaggtgat caaaatggga gtaaacgaaa
     2521 tgatgaaagt cagattgagt ttttaaatga taccttcctc agaacttcaa aacgaattca
     2581 aagattcgtt gacaaatctt gagcaagatc atttaaaaaa atattttctc ttcatttgac
     2641 gaaaaatgtt tagaaatctt atataagtga agataaagta attggtgtaa ttaattgtag
     2701 agaatagcct ttgagcaatt gaagcgatac aattatttag aaaatgaact tcctccagca
     2761 tactacatga ttccttacgg gacgatgtta aagaagaaag caagaaattt tagaagtatt
     2821 agtagataaa ctaatgggct tttctcaatt taagggagta gcccaaatgt ttatcaaata
     2881 tggtgcagat acagtagcaa ccatgtttac agaagaatat caagtagact attatgaagt
     2941 aatagataaa tgtgtacatt ttgtttatga aaaagtgtgg aattttatga attttgcgat
     3001 aagtgaagaa atcttacgag tacaagaaaa cagaacagga atatttacca gagacagaaa
     3061 gcttaatttg attgcgcatt atatacggaa tcgctggtca tcaataccaa attttaatta
     3121 taaaaaataa ttatgaacaa tcttttatac aatatacaca ataaatcaag tagtatttat
     3181 gaaaaaagac ttcaaaaaca gaatgatttt ctatcagtct tagaaattat agagctaaaa
     3241 attttggtca cgcgtagaaa cgcgtttttt ttgttttttt gatagtaaag aacgtacaaa
     3301 acaatgagaa tttaaaagat tgtactacca gtttttttac cttttcattt gagtttagag
     3361 taagtaaaat attactataa aaacacatat tcttgaagaa atcttttttt tgctgtattc
     3421 atttagatta tgatagagaa aatacgtgag agggggacct gggatgtggc tatctgatga
     3481 aaaaattcag tggcgtagct atcagaagcg tatattgact aactttgcta cttatttaaa
     3541 taacaattca gtccatctag tggctccacc aggggctggc aagacctttt taggaattga
     3601 gatgatccga cgtttggatt gtaaaacctt aattttggtt ccttccctaa tattgaagaa
     3661 acagtggtta acaacaatcg aggaatattt cttagtaaat ccagcagata gttgctatct
     3721 ttctggggcg attgaacatc ctagtcatat tacagtggaa acgtatcaga ccatttacaa
     3781 ccgactgaag gaggagccaa cttatggtga tcagctggag ctgttagttt tttgatgaag
     3841 ctcatcattt aaaaaaaagc tggggtgaat tgttgattga cttgaagaaa aaacaaccga
     3901 atctgttgac gatttccttg acagccacgc ctccgattga tagctctcag acggagtgga
     3961 agcaatatat tgagttaaaa tggcctgatt gacgaagaaa ttagcattcc cgagttggta
     4021 aaggaggagg ttctatgtcc tcatcaggat tttctatatt tgattccggc taatcaggaa
     4081 actactcagc attattttac ctttttagag aaacaaaatg agctggtgga agcgttaatg
     4141 gaaagccaag agacggtgga ttatttgctt tctttagaat ttgttcagca tccgcttgag
     4201 cattcgaagg aaatttatga aaattttgag ctgttcacta gtgcattaat ttacttgaat
     4261 catcataact atgaattgat acccgaacat tggcagctat taggcagttc taagcagcaa
     4321 ggtaaaaaag cttcagttcc atctttaggt aaaaaagagc tgatcactat ttatcagcag
     4381 atgtttgaag atcaaccgga attgcctatt ttttatcaat tgaaaaaagc tggatgattg
     4441 tttgatgagt cgctgcattt gtttccagag tatccagaca ctgttggtag tcaggcgatt
     4501 cctgagaaaa agcaagcagt ttgtcaaatt ttgttaaaag aagaagttca gctgaagcaa
     4561 gaactgacag cggtcatttt gatggatcgt atttgcaagg aggtattagc aggcagtgat
     4621 ggtaccttag cttatggcgt ggttcctttg tttgatgaag ttgttcgtct attaggtaac
     4681 actcagcaaa tcggtgccat ctgtggagag ttcttactgt taccgaaaat gctagtaact
     4741 gttgagctgg cagaactagc gacgggtatg aatgaacatc cttatatgtc gggttatttt
     4801 tatttgacgg tggatgacaa aaaccgccaa gcattgatgc aagcagttac tcgcctatta
     4861 accgaaaaga agttgaatat tttggtagga accgttagtt tgctaggaga aggctgggat
     4921 tgtcctgcaa ttaatctgtt gatcttggct aatcaagctt ccacttttgt gcaaacacaa
     4981 caactacgag ggcggggctt acgcctaggt gcacaaaata aagtaacgaa catttggcat
     5041 ttagcgattg gattaccggg agttcctctg tctgaacagc ctgatttgaa aagaatgcta
     5101 cggcgtttgg gcaatatttc aggtctgacc tttgaatctt caccaaaaat tgagagtggt
     5161 ggcgatcgct ttgctttacc actggaatat acgaccacca cgatggaaac ttatactcag
     5221 tcgatgttct atttttcttg tgaacgaagt cggttgaaag aacaatggag tactgcttta
     5281 gctgatggct ggcacctatc tatgccaatc gtcgttagga aagtcgatca ggtaaagcaa
     5341 aaacaagaaa cttcattgcc gatagtagta aaaaatatag agcctctaag cttctggtca
     5401 gctatattta aaggtaactt gctggaatat ttccaaattc gtaggcgaca aaagctatgg
     5461 gaaaaggaat gtgcccgcaa aaaaagcttg gccatatgcg ttcaaacgac gttgcagcgc
     5521 cttggtgaag ttagtgatag aaaagtaatc gtagcggctg atcagcagga atttagatgc
     5581 tatcttgaga atgctggcta tcgagaagga cagcttttta ctgaagctat gagggagctt
     5641 ttagcaccgc tggagaatcc gcggtattta atcgaaggga aaaagatagt tctgacggta
     5701 ccagaacgct ttggccgaaa taagcaaatc gctaaccttt ttctaaacga attaaatcaa
     5761 tcgtcgctgc ccaaatgtca gttggtgtat actagaaatc ctgaaggacg ccaaaagcta
     5821 gtgacttatc gaatgaggca agtcttccag acccgcgatg aagtaattat tcaaaaaaac
     5881 atttggacgt aagttgatga aaataaattt tgctagagta gaacaagtcc gagaattaag
     5941 ttgctactta actgataatg ccaaggacag gagaatcttt tcttgacgac tgaaagaaac
     6001 tatttgactt atataacggt gtgtgatgaa tatttgcaag gcattctctt aaaaagtact
     6061 cgtaatagtt tttttgaaaa caaaacagta ctttattaat aggactgaca gagcgatata
     6121 agaacatctg cagacagagg tgaatgcttt tctcatgatc gatgaaacaa tggtacttcg
     6181 gatattcttg cttttagttt gaaccgttgt gattttcttt gattcctaaa agtagtttta
     6241 tagaatgaaa atcaagcata cggaatgctc ctttgattgt gttgtggtga ttcaatctaa
     6301 tggagcttct atatgttttt ttatttgcaa aaagaaccga tagaatattt ttatcagtct
     6361 tagaaattat agagccaaga aatttcaagc acacataaaa acgcgtgttt tatgcatttt
     6421 atgagcaaaa ataactaaag accattacgt aaaacatagg taaatcagca ttcaatctca
     6481 aattatgtga tcaaatgttg atattatttg attataagtt ttcttttgct attatcagtc
     6541 tgcaaataaa cagattatag agtatgaatg tagatagttc aatctttgtt tacgacctca
     6601 catgtttttt tactcaagag aaagtaatat aaagtgacta tatttgacag aactttacat
     6661 aaaaaaagta gttagttttc tgtagtaatc agaaatgtaa ataaattaaa aaaaagaaat
     6721 ttagcagtta tatattttta aaatattttg ggtgagtagt caatacaaca ttttgttcat
     6781 caagcattaa atgaagatgc aaggatgtta agaagttagc ttcttcaggg tcttgcatgg
     6841 tcaaggcttt aaatagttga tgatgttgtt ccataatttt ttcccatttt aatccttccg
     6901 tttttactcg taaccaacga aatcgttcca agtgcgtatt attatcttcc aaccattgcc
     6961 atacttcttc ccgttctgca atttcaaaac aagctttatg aaataaatta tcattatgaa
     7021 aaaaactacg agcattcttt tgactaacag ctttttcttg ttctgcaatt atagtttcta
     7081 aaacttgttt cttttgatta ttcatcttgg cacaacactc tagcataatt tgtcgttcta
     7141 aatgttctct gacaaaacga gcattttttg ctaaatctaa gtcgattaat gagatataat
     7201 ttccactttg aggaattgta tcaacaagtc cttgatgacg gagttgaatc aatgcttcac
     7261 gtattggagt acgaccgata gaaagcgttt cttccaattt tttttctgat actttttttc
     7321 caggttctaa gtcagcgtag ataatttgct gacgaataga ttgataagct tgattttgga
     7381 gactagtttt aattgtcata tttaatccct ctcaaaattt catttttgaa tttgatattt
     7441 tagttctatg ctcgttatat aatatcaaat ttcaaagtaa catactagct attaaaaaaa
     7501 caaaaaatac tcaaataaga tcaaagagaa attaagcttt aatttaagtt agttctaaaa
     7561 aatatatatt ggtgtgcttt tactaaaaaa agacacaata attctgacat ggtagaaaaa
     7621 agaatatttt tagttttttc ttattgacag cgattacatt cgattttata ctacaattgt
     7681 aacggaaact aacatatcag tatggagggg taaatatggt tttgattaaa gactggcaag
     7741 attctatcga aggattgaac aaaatgcaag ttcaactacc gtattttaat gtcgaagcgg
     7801 tcaaacaaga aggtcagaaa catccagtgt ggcttcattt tggtggagga aatctttatc
     7861 gaggatttca tgcagaaatt gctcaatgct tagcagatca aaatgaatta gaatccggag
     7921 tagttgtgtg tgagacattt gatgaagaag tagtagaaaa agcttatcat gactacaaca
     7981 atgatatttt ggagattgtt atgggtgaag atggtgaact caaaaaaaga cttttagcag
     8041 taacagctga tagtttatat tgcaatccaa agaatataac ggactatcaa agagtggtta
     8101 aatattttga agatcctaca ttacagttta caacgtttac tattactgaa aaaggataca
     8161 gtttaaaaga ttctaaagga caattttttt cggtagtgga agcagatata aaatctggac
     8221 ctaccaatcc tcaacatacg atgagtattg tgacatcctt gttgtatagc aggtttaatg
     8281 ctggagagtt accgattgcg atggtttcta ctgataattt ctcacagaat ggtcaacgtt
     8341 ttcaagatag catttttatg attgcagaag cttggcaaaa aaaaggatac gtttctgaaa
     8401 aatttattgc ttatttaaag gataaagaaa aagtgacttt tccttggagt atgattgata
     8461 gaattacgcc aaatccttcg gaaagtgtca aaatggattt attgaaggcg ggtttcgaag
     8521 acttagatat aattcatact gcgaaaaata ccaatatcgc tgcttttacc aatactgaaa
     8581 aagtccatta tctagtcatt gaagatagtt ttccaaatgg tcgacctaaa ctggaagcgg
     8641 ctggtgtgat tatgacagat agagaaacag ttgataaagc agatatcatg aaggttacga
     8701 catgtttaaa tccgctccat acggcattag caataacagg ttgtttgttg gggtataaat
     8761 caattcaggc agaaatgcaa gatgatgatt tggttggctt aattaaaggg ataggttatg
     8821 tagaaggctt accagtagtt gaaagtccag gcgttattga tccaaaacaa tttattgatg
     8881 aagtaatcaa taaacgtttg cctaatccct atattccaga tacaccgcaa cgaattgcaa
     8941 cagatacttc tcaaaaagta gcgattcgct atggtgaaac aattaaaaag tatgtcgaaa
     9001 aagacggaaa tgcagaaaat ctaacattta ttcctatcgc tattgctgct tggttacgtt
     9061 atttattggc agtcgatgat caaggaaaaa attttgtgcc aagtcctgat cctttattgg
     9121 tagagttgca ggaaaaattc tcagtattga aattaggtga acaagatcct gcaaagattc
     9181 attgtgcggt acaggaaatc ttagaaaata aggaaatatt tggtttagat ttatatcaag
     9241 ttgagatagg aaagaaaatt gaaaaaatct tttgcgaaat gctagtaggt cctcaagcag
     9301 ttcgtaaaac aatcaataaa tatataacag aagttggagg aaagtaacta tggaaatgac
     9361 ttttagatgg tatggggcaa atgaagaaaa aatctcacta caagaaattc gtcagatccc
     9421 agcaatgaaa ggaattgttg gaacattaat ggatatacca gccggagagg tctggccgag
     9481 agaacgaatt aaagctttaa aagatgaagt cgaagccaat ggccttacct tgaaagttat
     9541 tgagagtgtt aatgttcatg atgatatcaa aattggtttg ccaacacgag atcaatacat
     9601 cgagaattat aaacagacga ttcgcaattt ggcagaattt gggattgagg ttatctgtta
     9661 taattttatg ccagtctttg attgggtaaa atcagatttg gattatcatt tagatgatgg
     9721 gtcttctacg ttagcctata tttcaaaaaa tattcctact gatccagagg aaattattag
     9781 aaaagtagaa aattctacta ataattttga attgccgggt tgggaacctc atcggttatc
     9841 acaaatcaag caattattag aagcctataa agaagttgat gaagcgaaat tgcgagagaa
     9901 ctttgcatat tttctcaaag caattatccc gacatgtgaa gaagttggga ttaaaatggc
     9961 agttcatcca gatgatccac catattctat tttcggatta cctcgtattg ttaaaaatcg
    10021 tgacgattta gaatggttgt gtaatgtagt ggattctcct tctaatggta ttacgttatg
    10081 tactggaagt attgcagaac gaccagataa tgatgtctat gaaattctat ctgaattttg
    10141 tcaacgggat cggattcctt ttgcacatgt tcgtaatatc aaatatttaa gtgataatgg
    10201 actcgatttt tatgaagcgc cgcataaatc agaatatggt tctctagata tgtataagat
    10261 attaaaaaca atgtacgata atggatttaa tggctatatt cgtccagatc atggtcggat
    10321 gatttgggga gaaaccggta gacctggtta tgggttatat gaccgagctc taggtgcagt
    10381 ttatttaaat ggtatttggg aagcgcttga aaagggggcg agtaaataat tatataatgg
    10441 agtctattta gaagaaaaat tttctcttct aaaaactaag ggaaggaaag gagtgcttaa
    10501 atatgttttt agataagaac tttttattag agaatgaaat agctaaaaga ctttttcata
    10561 aatttgcaga acaccaacca atcatcgatt atcattgtca tttagaccca gcatgtttgc
    10621 tagaaaataa gcaatacaag aatttgactc aggtatggtt aaatgataaa ggagctggag
    10681 atcattataa atggcgattg atgcgagcta atgggataga agaagagttg atcagtggtg
    10741 caggtaatga ttatgaaaaa tttttagctt ttgtaaaaac agtggagaag gcgccaggta
    10801 atcctatttt tgaatggtct catttggaat tacaacgtta ctttgatatt gatttgacaa
    10861 tttctgagaa gaatgcacca gaaatttggc gacgtgccaa cgaaaaaatc caaacggatt
    10921 cctatcgccc aaaagaattg ctgaagatga tggatgtgaa agccatttgt acaacggatg
    10981 atcctacaga tgacttgcga tatcatactg agctttctaa acaggataat tctttcaagg
    11041 tattacctac tttccgtcca gataaggtct ggagtatcaa atcatcagga ttttcgaact
    11101 atattttaaa acttagtcaa gcagcgggta taccaatcaa aaactttaat gggttgaagc
    11161 aggcattgag ccaacgagtg gattattttc atcaaattgg ctgtcgatta gcagatcacg
    11221 gtttgaatac ttatttttat gaaatagcag atgaaaaaat actcgacaat ctgttaataa
    11281 aagctttagc aggaaaaacg gatttccatt tatcagaaat tgcacaattt gttacaggta
    11341 ttcaattaca tttgatgtct gagtatacaa gatatggttg gacgatgcaa atgcatttaa
    11401 atgctttaag aaataattca agtatattaa aggaacaaat tggagtagat gtcggtggtg
    11461 attcgatggg ggatcaagct agtttgacac aaaatgttgt agcattgttc gacactgctg
    11521 aaaagagtgg gtgcttacca aaaacaattt tatattcatt aaatccaacc gattggcttc
    11581 cactagcaac agcaatgcaa agcttccaag ggggtatgat tcaaaaaatc caattgggat
    11641 gtgcttggtg gttcaatgat acagctgaag gaatgcgtca acagttgact acgatggcgc
    11701 aacaaagttt gttgtcgaat tttacaggta tgttgactga ttctcgaagt tttctaagct
    11761 atcctcgcca tgagtatttc cgtcgagtat tatgtcaatt actaagcgag tgggttaaag
    11821 caggtcgaat tccagatgat gaaatgtatc ttagtcagat agtggagaat atttgttatc
    11881 gtaatgcgta taattatttt aatttttttg aaatcgaaag tagaaaataa tacaaaaaat
    11941 aaaagcttaa atttatttat tgtaagttag tacaactagc tagagtatgt atccataaaa
    12001 gaaggagata gatatgaaaa aagtagaaat tttaagtaag ttagaagcgg ctggagtcat
    12061 tgcggttgta cgtggaaaaa ataaagatga ggccattaaa gcaataaatg caattactgc
    12121 gggtggaatt aaagggattg aactaacttt tacagttccg aatgctgaag aaataatcaa
    12181 agaaattgct aaaaaatatg ctgatcaaaa agatgtaata attggagccg gtacagtttt
    12241 agatgcgttt actgctcgta ttgcaattat ggctggagca gaatatatcg taagtccatc
    12301 ttttgatcga caaacagcag agatttgtaa tttatatcaa attccttatt taccaggttg
    12361 tatgacaatt actgagataa aggaggcact taccgctgga gtagaaatca ttaaactatt
    12421 tccaggttct gcttttggtc catctgttgt aaaaacgtat caagctccat taccatatgt
    12481 aaatataatg cctactggcg gagtaagtct agataactta gaagagtggt ttgaagcagg
    12541 cgttgttgca gttggtgtag gtgggaattt gttggctcct gttgaaaaag gtgattttga
    12601 agaagtaact cgaatagcta gtaaatatat agataagttt tatgagataa agaattatta
    12661 ggagaaaaaa taaataatac tataaaatgt aaaaaataaa tactaactct ttatacttca
    12721 gagtatagta cgtaagttgg cagttcaaca gaatgaaaac gcttaacaat aggaaggagc
    12781 gcaaattata tggaaagaaa atttggtcta agagacaaaa taggatatat gtttggagat
    12841 tttgggaatg atttcacttt catttttgct agttcttttt taatgatttt ttatacaaaa
    12901 gtattgggga taagcggagc taccgtagga acattatttt tagtagcaag atttattgat
    12961 gcagttacag acataacaat ggggcgtatt gtggatggat caaaaccatc taaaactggc
    13021 aaatttaaac cgtggatttt acggatgagt ggtccagtcg ctttagcaag ttttttaatg
    13081 tatcaaacat caatggcatc agcttctatg agtattaaag ttatatatat gtatgtaaca
    13141 tatatactat ggggaagtat tttttataca tctattaata ttccttacgg ttcaatggca
    13201 tcagctatca cttctgttgc agaagaaagg acagcgttat caacatttcg tagtatagga
    13261 gctaccttag caggtatgtt cattggcgtg ttaacaccaa tttttattta tacaagagat
    13321 agttctggag ctcaaattgt acgtggtgga tcaactttta caattgtggc aggagtattt
    13381 tcagtgtgtg cagttatttg ttacgtaata tgttataaat taactgtaga acgggttaaa
    13441 agtgatgaat cgcagttaaa agtttcattg ttgaaatctt taaaaggatt agctaaaaat
    13501 agagcgttgc tttctataat agctgcagct atttttttgt tgcttgctca gcttttaaca
    13561 atgagtatga acaattatgt atttccagat tattataata atgcacaggg aatagtttta
    13621 atgaacttta tcaatcctat tttgatgttg gtacttgttt cacctgtaag tttaattttc
    13681 agtaaaaaat acggtaaaaa agaattggcg tcagtaggga tgttcttctc tgctattgta
    13741 tatttacttt tatttttgat aaagcctagt aatatgtatg tattcttagt tcttacttct
    13801 gttggatata tggggttagg tatttttaat acagtcattt gggcgaatat tactgatgta
    13861 atcgatgatc aagaagtgaa atctggccaa agagaagatg gaactattta tgcagtatat
    13921 tcttttgccc gaaaaattgg acaagcttta gctggcggtg tcggaggttg gactctttct
    13981 ataatagggt atgattcgtt agcaaaagta caaagtggag aagttttaca aaagctgtat
    14041 aactcttcaa cgttgattcc agcagtttgt ttctttgttg taggattgat tttatttttc
    14101 gtatatccat tatctaaaga aaaagtatta gaaaattata atttgctaaa tcaaaaacat
    14161 aactaggaga aaaaacatga acagttggat attttgtatt gattcagatg gttgtgtcat
    14221 ggatacaatg acttataaac acaatctctt tttcgggcca attgctgcag agatttttga
    14281 tgttcatcat agggaaaaat tcctacagga ttggaataat attaatttat attcagagac
    14341 tcgtggaata aacagatttt taggtttagt taaaacatta gaaaaaaatg atgtaccatc
    14401 gattaatcga ttaaagcact gggtatctgt aacaaaagaa ttgtctaatc catctttgaa
    14461 gagggaaata gaatactctc agtcagagga tttattaaaa gctttaaaat ggtcggaaga
    14521 agtcaatcga cagattagtc aagctaaagg attagataaa ccctttttaa gtgctagaga
    14581 tactataaaa gctctgaaaa aatatgggaa gataattatt gtaagctcag ctaataagga
    14641 agctattcaa gaagaatggg aaagacatga attattatcc ttagttgatg aactgtgttg
    14701 tcaagataaa ggaaaaaaag aagacattat tcgaagtgta attgaaaatg gttgtgattt
    14761 ggataagata ttaatgattg gtgattctcc tggtgatcta gaagctgcaa ataagaataa
    14821 agttttcttt tatcctattt tggttaataa agaaaaagaa tcatgggaaa acttaagaac
    14881 tgatatttta tttaaatgtt tatcgggaga gtatatagat attgaacaaa aatatataga
    14941 tactttttgg aaaaatctta ctaataagta gaggagaaag aaaatgacta taaaagtaga
    15001 cttacgaaaa aaaccatatc atttagatga agatgctatt tcttggattg aagagacgat
    15061 tgatgctatg actttagaag aaaaaatagg tcaattattt gttaatatgg gatctagtcg
    15121 tgataaaaat tacttaagta gtatggtcaa tcagtaccat attggagctg tacgatataa
    15181 tccaggaact gctgatgagg tatatgatca aaactatatc ctacaaacag aaagcaaaat
    15241 tcctttatta attgctgcta atacagaagc aggtggtaac ggagcttgta cagatggtac
    15301 cgaagttggt gtagaagtaa aaattggtgc aacaaatgat gcgaaatatg cttatgaatt
    15361 agggagagtt tctggaattg aagcatcggc tattggatgt aactggagtt ttgctcctat
    15421 tgtagatatt aatagaaatt ggagaaatcc tatcatttct agtcgatctt ttggacaaag
    15481 ccctgataaa gttttagaac ttagtttagc ttatatgaaa ggtattcaag agagtggaat
    15541 tttgcctgca gctaaacatt tccctggtga cggtattgac gaacgagatc aacatctatc
    15601 ttttagtatt aatagtttaa gtacagaaga atgggataat tcttttggaa aagtatactc
    15661 aggcttaata gaagctggat taccttcaat tatggcaggt catatccata tgccagctta
    15721 ttcaaaacat tttaatcttg atctgaaaga tgaagattta cttcctgcaa ctttatcaaa
    15781 agaattaatc actgacttac ttagaggaaa actcgggttt aatggattag tagttactga
    15841 tgctagtcat atgttaggaa tgactggggc aatgaaacgg tcagaattat tgcccacttc
    15901 tattgcggct ggatgtgatc tattcctatt ttttaatgat ccagatgaag actttaatta
    15961 tatgatggaa ggttattaca aaggaattat ttcagaagag cgtttacgtg atgcattaag
    16021 aagaatttta ggcttaaagg ctaaactagg tttgcataaa aaagaaaaga ataaaatttt
    16081 gttaccgaaa caagaagcta tggaaaaaat tggtttaatt gaaaataaat caatttttaa
    16141 agaggtagct gatcaagcaa ttacactagt taaagataaa caagatattt ggcctattgt
    16201 accagaaaaa tataaaagga ttttactggt agatgtacat ggggtaaaag gtgggtttgg
    16261 tgaagtagtt gctggaaata gacctcaggc cattgattta ttgaaacaga ttttgtcagc
    16321 aaaaggattt gaagttgaaa aatacatttc acctgaagaa gaattgaaag gattatcgca
    16381 agaagaaaaa attaaaaata tagctggaat atatgctgct aagagaccaa tcaaagattt
    16441 aactgaaaaa tatgacttga ttattaatat agctaatgtt aacccaggta cagttcaaag
    16501 aattgtttgg ccagcaagta aaggtactcc ggatattcca ttttatattc atgaaattcc
    16561 aacaattttt gtatctgtac agtgtccgtt tcatttagct gatgtgcctc aagtaaaaac
    16621 ttatatcaat acttatgatg gaaaacaaga aactttagaa atattagtgg acaaactaat
    16681 gggcctttct gaatttaagg gaataagtcc aatagatgct tattgtgggt taaaggatac
    16741 atttatttaa tatttaaatt atataggagt gatttacatg ttatatccca ttatgacaga
    16801 gacgaggcaa gtaattgatt taagcggtct ttggagattt aagttagata caaatgatga
    16861 cttacaagaa gaacttgctt tagaaccaat ggttgaaaaa gagagttatc caatttcagt
    16921 tccttcttct tataatgatt tatttgaaac agagaacatt agaaatcatg ttggatgggt
    16981 gtggtatgaa aaagaagtga ttcttcctaa aaaattatta gaagaaagat tagttcttcg
    17041 ttttggatct gcaacacatg aagcaaaagt atttttgaat ggaaaattga ttaatcaaca
    17101 caaaggaggg tttacgccat ttgaatcaga aattaatcag tttataaaag atggaagtaa
    17161 cagaattaca gtggctgtaa acaatgttat tgacgatagc actttacctg tagggatttt
    17221 gaaagaaacg atacaggaag atggagacat aaaaaaagaa aatttggtta attttgactt
    17281 tttcaattat gcaggcatta atagacccgt taaattgtat acaactccga aagactatat
    17341 aaaggatgtc actattaata caaaaataat tgattcagat gcaaatgtag aatttaatat
    17401 cgatacagaa tggaccggag aaattctaat agcaatctat gacgaagaag ctcaacttat
    17461 agcagaagct aaggggaaag aaggagtgat tcaacttaag aacccaacac tttgggagcc
    17521 tctaaatgct tacttatata gtatgaaagt tagtttaatt aacgaaaata aagtgattga
    17581 tacgtacaca gaagaatttg gcattcgtag tattcaggtt tcgaatggga agtttttgat
    17641 taataataaa cctttttact ttaagggatt tgggaagcat gaggacacgt atattaatgg
    17701 tcgtggtttg aatgaagcag taaatatcaa agattttaat ttaatgaaat ggatcggtgc
    17761 taattcattt aggacttcac attatcctta ttcagaagaa attatgcgct tagcagatcg
    17821 agaaggtatt gtagttattg atgaagtacc tgcagtagga ttacatttga atttcatggc
    17881 tactttatta gatgacaacg tagaaaaaca tgatacttgg aaagaaatac ggacagaaaa
    17941 tgctcacaaa atggcgatta aggaattgat agaacgcgac aagaaccatc cgagtgtcgt
    18001 aatgtggtct attgcaaatg aaccagattc ggattctgaa ggcgctaaag aatattttga
    18061 acctttagtt gaattgacta aagaacttga ccctcagaag agacctgtaa caatcgttac
    18121 ttacttaaag tcaacaccag atatttgtaa agtaggagat attgtcgatg ttttatgttt
    18181 aaatcgctat tatggttggt atgtttcagg tggagaccta aaacatgcaa aatatctgtt
    18241 gaaaaaagag ctagatggat ggatggaaag atgtcccgat acgccaataa tgtttacaga
    18301 atatggagca gatacagtag ctggacttca tgatacagta ccaacaatgt ttacagaaga
    18361 gtatcaagtg ggttattacg aagcaaatca cgaagtaata gataaatgtg acaattttgt
    18421 tggtgaacaa gtgtggaatt ttgcagattt tgaaacaagt caagggattt taagagtgca
    18481 aggaaataag aaaggaatct ttactagaga tagaaagcct aaaatgattg ctcactatct
    18541 aaagaaacga tggtcatcaa taccaaattt cagttataaa aaataattta ccttttataa
    18601 ggaatataaa aaaggagtgg ctgcttaatt gccaactcct tttttttata aaaagatttt
    18661 ctgtttaaag aaggatattt ttttctagtt ttttcgaaaa aaaggcgctt tctgattgtt
    18721 gtattagtgg aaaggggaaa aaaggagcat atttatcatg caacaaaatt acaatgagaa
    18781 atctatacaa catcaatttg atgcctactg taaaaaagta ttacgaaacg aaactaagtc
    18841 tattcgaaaa cgaaacatta acataacaga taatgaggaa ccattgacga attcaaatga
    18901 aggtaagtac tcccaaactc attttgatga acaagatgtc tatttcttat ttggaatgga
    18961 aatattaatt tcagatcaaa agttatcggc agccattgat catttatcgg aaataagaag
    19021 aaaggttatc ctactttatt attttgcagg ttttaatgat actgaaatcg gtaaaatcct
    19081 caatatgagt acaagtggta tctggtatca acgaaagaag gcagttgaac aattaaaaat
    19141 ggggtatgac ttatggtaga caaccccaaa aaacggcagt atccattagt tcctctatcc
    19201 attatccaat ctgctctaga agaagatcca atttcaatca agatgatact caaacattat
    19261 catagttata tagctaagtt atgtttgaca aatggattta atgaggcggg gcaatttatt
    19321 acctatgtag atgagtatat gctaagacag ctagaaatta aattgataga agcgatctta
    19381 aaatttaaaa ttaactaact aaaaagtccc ctttccactt attctgttta aaagaatctt
    19441 ctaatagtta aaggattagt agattctttt aaacagaaaa cctattgatc attgaaattt
    19501 aaatacattc tatcagtacg ttaacacttc aaggagcata ttttagttgc gccatgattt
    19561 cttactgtaa agaaggagcg ataaacattc taaaataatt tttatagtca ataaaaagcg
    19621 atgatttgaa gttgggataa tgatacttcc gtaaggctcg ccgtaaactt ggagagaggt
    19681 gaaattccta tgtgcttttg acaaaagcag ctgattaaga attatttcca tatgttgatt
    19741 tcaacatttt ttctattatt tttaagacca aattcagtga ggcaaatagc atcatgtaaa
    19801 tttagttttt ctattgaacg ttctccactt tttagttttt ttatagtact caaatttaat
    19861 cctgtatgtt tagagatctc atttgcagta tggttttgta aagtttctcg gattattgtt
    19921 atattagcaa tttcattacg cataattatg ttcctttctt atcaaaaata tcaatgacta
    19981 cagtgggctt ttagtgaaaa aaaggggtac tacagtctct tttgaaaaca aaaaaataca
    20041 gtatatacgt tttctaaatg ctaagcacca acgtttacac tgtatttaag ggtttggcac
    20101 ccaatgggct gtgaggggct cgaacccgcg acccgatgat taagagtcat ctgctctacc
    20161 aactgagcta acagcccaaa ttaatacttt gttattctaa cacctaaaat aaaaaagtgc
    20221 aatacttatt atcagaattg agagtgaatt ttatggattt tcaagattat gaaaaaatat
    20281 ttgaaagtta tccatcgata ttgaaaatta ctgatttaac taaaatgtta aatattggaa
    20341 aaaataaagc ttataatttg ctgaaagata gatcaataga aaatataaaa attggagcaa
    20401 ggtacaaaat acctaaaaaa gctgtcattg agtatatatt gaaaatacaa gatgaaggta
    20461 aaaatcatgc tataataaaa tagtcaaaat ttttgccgat atttcagttt aagaggagag
    20521 attgaattga aattaagagc aaacataatt gaaaaaagag gaacattaca agtagttgta
    20581 tcttataaag actctaacgg aaaaattaga caaaagtgga aagatacaaa attaaaaaag
    20641 aaaggaaata aaaaacgagc agaaaaaatt agagatgaat ttttactaga attggaaagt
    20701 caattagcta tggaatgtga atgtgctaat ccaaatttat tgttttatga ctatttgctt
    20761 aattatttgg aagtatcaaa aaaacaagta gcatttaaca catatgttgg gtataagcat
    20821 tacgtccata atcgaattta caagtttttt tatcctaaaa aaattaaact aaataggtta
    20881 aagccttatc atttacaaga gttttatcaa tatatgttaa atgataactg tacagcaaat
    20941 acggttattc attatcacgc atttatacgc aaagctcttc aagaggctgt tattacagaa
    21001 ttgataagta caaatgtagc agataaagta agaaagccaa aaaagaaaca atttgtttct
    21061 caagtttata atcaacaaga gatcttgaaa ttacttgaca ttttgtcaga agaaaaactc
    21121 tatttggtag ttcttttaac agcattttat ggattgcgaa gaagtgaagt tttaggatta
    21181 aaatggtctg ctattgattt tttaaacaaa aaaatcatga taaaccatgt aatcatagaa
    21241 aatccagaga atatgtcaca gttaataaag aaagatcaaa caaagaactc ttccagctat
    21301 cgtacattac ctttggttga aacgattgaa cgagcattag taaatcaagc taaatggcaa
    21361 caagataatc gtattctttt aggagatgat tatattttag aagataatga gtacgtattt
    21421 actatggaag atggacggtt gatgaagcct caatatgtaa cgcatcgttt gagtaagttg
    21481 attaagaaaa atggattgaa aaaaattcgc tttcatgact tgcgtcattc taatgcttca
    21541 ataatgttag atagtggcca gaatatgaaa tccattcaag aatggctggg acatgcatcg
    21601 tattcgacaa ctgctaattt atacacgcat ttaaccgcag gagtaaaaga tcgtgcagca
    21661 gaagcattag aaaacgcatt tgggtttaaa gaataaaaaa acaaaaaaca atatctttgg
    21721 ggtatcgctt cttgttttta gaattttttt gttggcaatt tgttggcagt atgttggcaa
    21781 tgaaaaaatt cacaaactaa aaacctattt ataaagcttt ataaaacttt tttcacaaat
    21841 agagcatttg gttaagagtc agctgctcta ccaactgagc taacggcccg tacaaaaagt
    21901 acttaataat agtagcacta agaattttaa aatgcaagac tacatttaaa gaaaatcaga
    21961 aaaatcttta cttccttata ggaaatcatt catagattat tcacatttgc tcgctattat
    22021 ataagcatac caacaaacaa agttcttgca agagaacaaa ccctaacact tttttcattt
    22081 tttactcctt tcccgccgtc tccccggcgg gtctcctgtt cttaacgata aaagacaaga
    22141 aggcttagcc ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc acctagttaa
    22201 agcaactaga ttgaatgttt atcataagtc tgatatcatg tttatgaaat gaaaaaagga
    22261 gcggttatga tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga actagaaaca
    22321 ttccgtctgc ctcactggga agaactccct gacttagaac tttatatgga ccaagtcaga
    22381 acgttagttg atcgttatct ttcacctgtg atacaaggag aaaagcatcc attgcttacc
    22441 tcttctatgg taaacaatta cgttaaacta ggtttgatac cagcaccagt taaaaaaaga
    22501 tataacaaag aacacgtagc atttttactt gcaatcacta cgttgaaaca agtattgact
    22561 attcctgaaa tcaaagaagg gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc
    22621 tataatctat tttgcgatga acaagaagca gctgtgtgga tggtcagcca attagcacaa
    22681 ggtaaatctc atccgcaaaa attcgaccag gcaacccctg tagaatatat tgctgtaaga
    22741 gcggcaactt tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt tctagaaacc
    22801 gaatatttaa aagaggagaa aacaaatgaa aaacaataaa atcgcaattt tagtagattc
    22861 aggtacggat gttccagaag aattgataaa aaaatacgag atgtatatga tcccattgaa
    22921 aattatttat aaagatcagg tatatacaga taaaattgac attacccctg aagagatcta
    22981 tcaacgtttg ccagaggaaa tccctggtac atctttgccg gatggtgagt cgatcacaga
    23041 aatctttgag caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa caatttccag
    23101 tggattgagc gggacatata atgtggttcg tttaattgca caacagcaag aagaattgga
    23161 agcatatgta ctagatacga aaaatattgg tattggtgca ggtttttctg cgatccaagc
    23221 agcaaaatgg ctggaagaag gagtagaatg ggatcaactg atcagtaatc tgaatgagtt
    23281 agtcgaaaga acaaaagttt tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg
    23341 acggatcggt ttagttgctt ctattgtagg aaccgcttta aagcttaacc caatcatctc
    23401 atgtaatgaa gaagggattt attacacagt aggaaaagct aggggacgta aaaaaagttt
    23461 ggataaaacg gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc
    23521 tgttgcccat ggggatgcga aagaagaagc aattgaaatg gaagcgagac ttaaaagaga
    23581 gttcccacaa gcaaaccaaa tctactttgg ccagatttct ccggcattag tagtccacac
    23641 tggtccggga ttattgggtg tcggtgtaca agttttagaa ggataaatca aaaaaaggaa
    23701 acactgaggt gaaaatcagt gttttccttt tttattgaag ttagaacaaa aaataataaa
    23761 gtattttgca ctttcatttt aattttctct tttgttttat aggcaaagat gaaacaaaag
    23821 cagaaatacg gtataatgag aaggtatgac aataatatat aagaggtggc tctttatgaa
    23881 aaaagtttta gttgtcgatg atgagaagcc gatttcggat atcgtcaaat tcaatttagc
    23941 caaagaaggc tatgacgttt atactgcata tgatggggaa gaagccttgg aaaaagttgc
    24001 tgaggtagaa cctgatttga ttttacttga tttgatgcta cctaaaatgg acggcttaga
    24061 agttgctcgt gaggtacgca aaacttatga tatgccgatc attatggtaa cagccaaaga
    24121 ttctgaaatt gataaagtcc taggcttaga gttaggcgcc gatgattacg tgaccaagcc
    24181 attttccaac cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc
    24241 tgcgaaggaa ccagaagagg cagcacctgc ggaattgacg attggtgatt tgacgatcca
    24301 tccagaagct tacatggtga cgaaacgagg agaaacaatc gagttaacac atcgtgaatt
    24361 cgaactactc ttttacttgg cgaagcacct tggacaagtg atgacaagag aacatttatt
    24421 acaaacggtt tggggttatg attattttgg agatgtacga acagtggatg taactgtacg
    24481 tcgtttgcgt gaaaagatcg aagataatcc aagccatcca aattatttag tgacacgtcg
    24541 tggtgtcggt tactatttgc gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc
    24601 gcttttttcg atcagtcaat tttaagatcg ctattacctt tattctgatt ttattgatct
    24661 cgatcgaaat tattggtgcc tactttatac gtggtttaga acgttccacg atcaatacat
    24721 ttattaaaga tatgaatcaa acggtggaat cattagcaac aacaatcagc ccggaattga
    24781 atcgaaaaga taatgcagat gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa
    24841 acagtgcgac tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg atcatccgag
    24901 gaacaaccga tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat gttgatatca
    24961 atgatttcac gactaaacgt tatgttgctt tagataacga cgataaacgg gtgaatatca
    25021 atgttcaacc gatcctgtcc ccgaccggcg atgccgttat cggggctctt tacgtcaaaa
    25081 gtaatatcga acaaaaatat tcggaaatca ataacacagc agtcattttc tttactgcct
    25141 cactaattgc ggcatttatt tcaatgatcg tatctgtttt agttgctcgt tcgattacac
    25201 agccgatcgg tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac tacagtcgga
    25261 aagtcaaagt gcatggacaa gatgaacttg gacagctggc tgataccttc aatcagttag
    25321 ctgaacgtat tgaagaagct caagatacaa tggaagctga acgcaatcgt ttggatagcg
    25381 tgctatccca tatgacagac ggcgtcattg cgacagatcg caggggtaaa gtcattacga
    25441 tcaatgatat ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc ggacaatcga
    25501 ttttgacact tttagatatc gaagaagaat atacgttgcg gaaattatta gaagatccaa
    25561 acgaaatggt acttgaccgt tcaaccagta cattggaaag tgatcagatg atcttgcgag
    25621 tagacttttc tatgatccgt cgcgaatcag gttttatcag cggattagta gctgtcttgc
    25681 atgatgtgac agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt
    25741 cccatgaact tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag
    25801 gtgcgtggaa agacgaggaa gtagcaccaa attttttgaa ggtaacgcta gatgaaacag
    25861 accgaatgat tcgaatgatc aatgacctct taaatctatc tcgcatggat acaggtaata
    25921 cccagcttca attagaatat gtcaatttca acgagttagt gaattttgtt cttgaccggt
    25981 ttgatatgat ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa
    26041 gagatctttg ggtcgaagta gatacagata aaatcatcca agtagtcgat aatatcatga
    26101 ataacgcgat caagtattca ccagatgggg gaacgattac ttgtcgctta ttagaaacgc
    26161 ataataacgt gattttaagt atcaccgatc aaggattggg gattcctaaa aaagatctga
    26221 accgagtgtt cgagcggttc tatcgagtag ataaagcacg tgcaagagca caaggtggga
    26281 caggacttgg attagctatt tctagagaag tgattaaggc gcatcgaggc gctatttggg
    26341 cagagagcaa ggaaggaaaa gggtcgactt tttatatctc gctgccatat gaaccttatg
    26401 aggaggaatg gtgggaatga gaatctctga aaaagtcgtt cgtataggac tcattttgat
    26461 ggttgcgtta agcatatact tctcttatgc aatctggctg agcccagcag gaaaaagttc
    26521 aatcaacgta gatgagacaa acactcaaat gatcgagtct caaagcaatc gaaaaacttc
    26581 cgaagtcttt cttccgcttc atgttacatg gcaccattcg ggaatgatcc aagaaaccaa
    26641 cagtgaaaat ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat ttggaaagct
    26701 ttctgaagta gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg
    26761 ttttgagttg gcctataatg cgcctttttt gctaagagaa tacaaggaag catttgattt
    26821 agatttagat gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta ctagaataca
    26881 attcgatcaa ggaaataaaa aaatccgatt tttgaattat tcgaaccatc tcgtatatga
    26941 agcaaatatt tcgttgaatt ggacagatat caataaagaa ttaacagcaa aagacacaaa
    27001 atggacagaa atgacaacag aaccatcgac gatcgacacg caatatgcta cgaaacattc
    27061 gatcaaactg aagaagtata gttatatcct gtcacagcaa ccctatacac tttttagaaa
    27121 tgcttttttc cagaaaccag atagcgtgaa aaataatgat gaaagcaatg aattgatttt
    27181 ttacggtggg aatgaaacaa tgtctatcca ttcggaaacg caagtagttg attttcgtgg
    27241 agagctgcca gagaaaaaag aaaatgctag tgtattttct gaaagtttcc cctatattag
    27301 caaattaggc agttctttag ggaacctcag atattttgac cgcagtgaca atacgatcga
    27361 ttatcggata ttcgtggaag gcttcccggt ttttgggaca gacagcagag gacagattgg
    27421 tgtggaagtt ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa
    27481 tacgattcaa gtgccgatac cttcagacga agaagtagaa cttgcaagta cgaatgaaat
    27541 catccagcag ttaaaagcta agggagcaga tagcaacaag attggttcag tgatcgttgg
    27601 ctatacatgg caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta
    27661 tgtgaaatat aaagataact ggtattcagc taacgacctt ctggaaaaat tacctggatt
    27721 ggaggcgaat taattggatt tcaaaaaaat cgaatggata tttttcgtcg cttttttagg
    27781 gttgaatgtg tttttattca gcagttatca tgacgcggta taccaagaaa acaatgtcat
    27841 tcgctctaat cagactgaat caatccaaca acgtctggct agcgacgata tcacttacac
    27901 ggggaagatt tcgtctgaga acaagcaagg ctattatctt agcgcggagc agaccaatct
    27961 tagcacttca ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt
    28021 gtttttagac ggtgatgttt tgacgaaatc tttatccagt tcagatgctg aaaagaacgt
    28081 gatcgatcca gataaaatta gtagttcctt ggaagatttt ttatctaaca aagacaatat
    28141 tttattcggt aatgattatg tgtacacgaa aaacttttct catttagaag aagaaccact
    28201 agaaatccaa gcaagccaag agtatgaagg catcccattc aatgatgata cagcaaaaat
    28261 cgttctttct ttagaaaaaa acgataatga ttatagcatc acgaaataca tgcagacgca
    28321 tttatctgat atcgagcagc tgcgcgaaaa gacggaactg tatacggaag aggaagcaat
    28381 caatacattg tacatcaata acaaaatacc aagaggatca aaaattttat ggcgtcagct
    28441 agcttattcc tgtattctga aagtccggga aaaaaatgtg tatgttcctg tttggcatgt
    28501 agcgatcgaa acaagcgata aagtggtcca aattgagagc gtcaacgctt tcagcaatac
    28561 gatcgtgaca aacaatactg taccaaaagt ggaagatcaa taaaaaagcg agtataatga
    28621 aaaaagttta caaagtaaga agggaagtag gagtcaaatg gctgaagaaa aaccagcttt
    28681 taaaatcagt atcctagcaa gtggaagttc gggtaattca ctttatatcg aaagtgaaaa
    28741 aaaacggctt ttggtcgatg caggacttag cggtaaaaaa atcacttcct tgatggctca
    28801 aattgatcgg acaccggacc aattagatgg aatacttgtg acgcatgaac atcgagatca
    28861 tatacatggc gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg ccaatcagca
    28921 gacttgggaa gcgatggaac cactgattgg aaatgtacca gtggaacaaa aacatctatt
    28981 tgagatgggg aaagtcagaa cattcggcga tatcgatatt gagagtttcg gcgtctccca
    29041 tgacgcagcg gctccgcaat tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt
    29101 gacggacacc ggatattgca gtgattatat gcgaggtgtc attgaaaatg ccgatggtta
    29161 tttgatggaa agtaatcatg atcttgagat gttacgcatg ggaccttatc cgtggaattt
    29221 aaaacagcgt attttaggag atagaggaca tctttccaac gaagacggtg cacttgtcat
    29281 gactgatgtg atcggtaatc ggaccaaacg aatctatctt ggccatctta gcaaggaaaa
    29341 caatatgaaa gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg
    29401 cgttggacat gattttgaaa tctatgatac agatccagat gaagcaactg agctattttc
    29461 gatttaaaca gttcaatcaa taaaaatata aaaagcagaa gataaaatca atcagggttg
    29521 gttttatctt ctatttttat ttttttagca tattaaaaaa ataaccgtat tatcgactga
    29581 ctacactgat gtgctgcttt tcatgtttct catccagtat ctcatctact aatgcaatcg
    29641 cgtaatctgc catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac
    29701 ttaacaaata agtacctgta cgtttgccat gtggatcaaa taaagaagct ggactgatat
    29761 aagtccaatg tagattttta gattgtttca attttaacaa tccttcgtac atattgaaag
    29821 ctgttggata gaagggtgca tttttaggta cttggtcaat catacgagtc gtcttttctg
    29881 tatctaagaa taacgaagag gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa
    29941 gcatctcaat caaatgggac aaagaagttt gatgcatttc ttcttttcct ttgggagcgt
    30001 tgaaagcatc aacgacaaca tcaaaagatg caagatcagt agttgtcaac tcaaataaat
    30061 ccttttgtat aaacggaaca tctgatggca atttttctgg atgacgcacg attgccgtca
    30121 cgtctaaatc tctggacaat gcttcgtcta aaataaatga acctgcatgg ccagtagctc
    30181 caatgagtgc aattttcata aaaaacctcc ttttttattc ctgtccattg taagcagttg
    30241 ctgaatattc gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc tttcatggtt
    30301 cgtgatcatt gggtatagtt aaattaagaa aactgattga taaaaataga agttgaagga
    30361 gataagaaat gaaagagtgg ttagatcgtg cctttttacc aatgataaca tctgttgtga
    30421 atcttagacc cttaactgct ttgcgtaatg gaatgactta cacgatccca ataattattg
    30481 ttggctcgct gtttttctta tttgctcatt ttcctgtgga ttacatcgct cagtggatag
    30541 acaaaacagg attttctatg tattttacac aagtatacca ggctacattt gatgtgatag
    30601 ccttttggat cgtttttgcc attgcatacg cttatgcaaa agaagaagac atcgatcgct
    30661 taccagtcgg cataacagcc ttgtctgctt atctactgct tattcagcca ctaaatactc
    30721 agatagatac tgtctggaca ggtgctgaag ggatactagg agcagttaca actgggctca
    30781 tcatcggatg gggctatgca cgtttagtaa gacttgcgaa agggtggaag caaagagaga
    30841 agatacctga aaatgtttgg cagtctttta cttcattgat tccaatcatt gttatagtca
    30901 cttttacgct tatcggttca gcggtttttc aacatttgtt cagaatgact gttgttcaag
    30961 gagtatggac atatgtccag cttcccattc aatcgttcat agacacgttg tttggtgtgc
    31021 ttttattagg tttttttgtt ccgtttttat ggttatttgg tcttcatgga tctgcgatgg
    31081 tcaatgggtt ggttagtccc gtcttgcaag cgaattcatt agcaaatgca gaaatcttag
    31141 cctcaggaca agagttgacg gtggcaaatg gcggacatat cgttactcag caatttctcg
    31201 atcagttcat gacggttacg ggtgctggat tgacgttagg ggctgtattt tttatgacgg
    31261 tttttgcgaa atcgaagaaa tacagagaat taggaaatct tgctctttta ccagcctttt
    31321 tcaacatcaa tgaatcaatt attttttcca caccgatcgt gatgaacccg atgatggctg
    31381 ttccttttat tttcgcaccg gtcgtttcag gattgattac ctattccgct ttatattttg
    31441 gattcgttcc tttatttaca gcagtccaag tcccttggac aacaccgccg atcctctcgg
    31501 gtttcttagt aggcggagtg cctgcagcga ttttacaagg tatcgtgttg tcaattagtt
    31561 ttttcattta tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac
    31621 agacaaaaaa tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt
    31681 cgctttagat tgggaaaatc accaatctag gatgacagga gtatttatag tcatgtttat
    31741 actagccctt tttggacagc taattgaaca agaacaacta atgtgttcat tcctacgtgt
    31801 gtgatcatcg atgtccagat ccgaccagtt gctttgtact ctaggctgaa gaagaaacct
    31861 aggaaaaagt agattagcag atgaccatca ttatgagcaa acgcaaacag caaagagctg
    31921 atcactgctc cgacccaaaa atttgaatat cgactgatga tccccagtat actgcggcgg
    31981 aatacgaact cttccatgat gggtccagca acagttgtcg ccaaaataaa agcgggtgct
    32041 tccatgatca tctggatgat attttgtgta ttctcggaag gggttgcttt gccaaacaat
    32101 acagattcta acatgacagc gatgctttgc agaatcaatg cgacaaagat tccaaccaat
    32161 ccataaataa caatctttgg agcagaagct ttttttaccg ctgattcgat cgctaagggt
    32221 tcacgttgct taaaataaaa aatagctagg acaacggcgc ctagtatata catgactgca
    32281 gttgctgtga tcaattcaga cgtagatttt acaagtccta tcgaagctag gatcaagggt
    32341 gagataaaaa ttagaccata gcaaaaaatg ctgaggaagc tatatttttt taaagacatg
    32401 taattcctcc ttttaaggga taacaagcat gcaaggcaag cttatatcga ttgatttcaa
    32461 ttatatcata atttcaataa gcagctgtca gaataaaatt tcttttcttt gatttaaaag
    32521 cttgcaaaaa agagggagaa tgagtattat taaaaatgta ggttagcact cgaaggttaa
    32581 gagtgctaac agattaaaaa aagagttatc ggagggattt atcgtgttaa aaccattagg
    32641 tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt
    32701 agcttctgct gctaaagaga aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg
    32761 tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt
    32821 cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg
    32881 aaaagatatc atggcgattg ttgagtaaat ttggaagata aaaaaataat caaaaataat
    32941 caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct
    33001 atgttacgcg gggtagataa attagcagat acagtgaaag taacattagg acctaaaggc
    33061 cgaaatgtcg tactagaaaa atcttacggc tctccattga tcacaaatga tggtgtgacg
    33121 atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa acttgtatca
    33181 gaagtagcat caaaaacaaa tgatattgca ggagacggta caacgacagc aactgttctg
    33241 acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa tcctctaggc
    33301 attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca taatatctca
    33361 actgttgttg attcaaaaga agccattgca caagtagcag ctgtttcttc gggttctgat
    33421 aaagttggac atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca
    33481 atcgaagaat caaaagggat cgaaacagaa ctagatgttg tagaaggtat gcagtttgat
    33541 cgtggttact tatcacaata tatggtaaca gataacgaca aaatggaagc tgttctagaa
    33601 aacccatata tcttgatcac agacaaaaaa atctctaaca tccaagatat tctgccattg
    33661 ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta ttgctgacga tgtagatggt
    33721 gaagcattgc caactcttgt attgaacaaa atccgtggaa cattcaacgt agtagctgta
    33781 aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca
    33841 ggtggtacag taattactga cgatctaggt ttagaattga aagatgcaac aatcgaaaac
    33901 ttaggaaatg cttctaaagt tgttgtagat aaagacaata caacgatcgt agaaggttct
    33961 ggagaaaaag aagcaatcga agctcgtgtg caattgatca aaaaccaaat tgctgaaaca
    34021 acttctgatt ttgaccgcga aaaattacaa gaacgcttag ctaaattagc aggtggtgta
    34081 gctgttgtta aagttggtgc agctactgaa acagaattga aagaattaaa attacgcatt
    34141 gaagatgcat tgaatgctac acgtgccgca gtagaagaag gaatggtctc aggtggtggt
    34201 acagctctag tgaacgttat cagcaaagtt tctgctgtag aagcagaagg agacgtggca
    34261 acaggtatca agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac
    34321 gctggttatg aaggatcagt gattgttgat aaattgaaga acgtggaact aggcactggt
    34381 ttcaatgctg ctactggaga atgggtaaac atggttgaag caggtatcgt agacccaact
    34441 aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca
    34501 gaagcagtcg ttgcagataa accagaacca gcagcgccag cagctcctgc aatggatcca
    34561 tcaatgatgg gcggtatgat gtagtaatta agaaagaacc ctattttaac agggttcttt
    34621 cttctttttt tgttttattt taagcaaaag gggcaaagaa ggggcaaaaa tatcaaaggc
    34681 tatttagttt atcaattact tgttgtttgg ctctttttgt tacatggtta taaatagaca
    34741 gagttgtgtt tgcatcagaa tgccctacac gctccataat ggctttaaga ggtacgccta
    34801 attcagataa tagtgaaacg tggctatgtc tgaatatatg tgaggataga cttttttcta
    34861 attccaactc ttcctctact ttatggagta tagcgttaaa tgagtgcagc gcaagcggag
    34921 tgccacttgt agatataaat atatattgat cgggatctgt gggtctacct gcaagaatat
    34981 tgtcagctat cacactttca atcaattctt ttgcacgatt gggtaattgc acttcacgtt
    35041 gcgaataagt attttttgga gttgttttta tagcattatc cattttcaca gatgtgtaat
    35101 ctaaggtccc attaatggaa attttcccat cctcatagtc cttcatttgc aaagctagca
    35161 attccccata tctcaaacca gttaaatata gaaactcagc tattatgccg tgtagttttc
    35221 tgcgaggatt ggagtatagc tgtttcagta tttgatcaat ttcttctttc tccagatatt
    35281 ttttattcat agaaagcctt cttttttctt cttccacttt tttaggatgg attttaactg
    35341 ctagcgcagg gtttctttga atgtattttc tatcgattgc atagtttagc ataacagata
    35401 gagttgtttt tgtttgtttt gtgtagttca gtgagaggtc accaaacgta tacatatctt
    35461 caattatctt attaatgagt gtctcatcaa tgtttctaac gatcgtatca tcgcttatgt
    35521 gcttagaaac atgtttcatc atcattggaa ccttcaaata gctagtacgt ttaacatgct
    35581 gcttataata ttcataccat tctttataca gctcaccaaa agtgatatct gatttattgt
    35641 aatcttcgag tgcttcttta atttttttat ctagaatttt ctgagctttt ttccacgctt
    35701 gtggtgaatt acttgtaagt gttgtagatt tttttcgtgt tttttctgta taaggatcta
    35761 catatctttc aataaacttg aattggccat ctttggtctg ttcaacccac acttttaaca
    35821 tctcctatca tttgctataa taggcataac aaatagacct atataggttt gttttctaaa
    35881 agcacgctct tactctggac ggt
//
LOCUS       DACDKJ010000022        34063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDKJ010000022 DACDKJ010000000
VERSION     DACDKJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.022"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IV328_001243"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IV328_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8937394.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IV328_001244"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IV328_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8937395.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IV328_001245"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IV328_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8937396.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IV328_001246"
     CDS             1642..2274
                     /locus_tag="IV328_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8937397.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IV328_001247"
     CDS             complement(2276..3181)
                     /locus_tag="IV328_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8937398.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IV328_001248"
     CDS             complement(3266..3919)
                     /locus_tag="IV328_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8937399.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IV328_001249"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IV328_001249"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8937400.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IV328_001250"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IV328_001250"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8937401.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IV328_001251"
     CDS             6168..7253
                     /locus_tag="IV328_001251"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8937402.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IV328_001252"
     CDS             7250..8314
                     /locus_tag="IV328_001252"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8937403.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IV328_001253"
     CDS             complement(8563..9366)
                     /locus_tag="IV328_001253"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8937404.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IV328_001254"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IV328_001254"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8937405.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IV328_001255"
     CDS             complement(10244..11251)
                     /locus_tag="IV328_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8937406.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IV328_001256"
     CDS             complement(11258..11902)
                     /locus_tag="IV328_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8937407.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IV328_001257"
     CDS             complement(12002..12760)
                     /locus_tag="IV328_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8937408.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IV328_001258"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IV328_001258"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8937409.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IV328_001259"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IV328_001259"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8937410.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IV328_001260"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IV328_001260"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8937411.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IV328_001261"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IV328_001261"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8937412.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IV328_001262"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IV328_001262"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8937413.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IV328_001263"
     CDS             18158..19027
                     /locus_tag="IV328_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8937414.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IV328_001264"
     CDS             complement(19153..20052)
                     /locus_tag="IV328_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8937415.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IV328_001265"
     CDS             complement(20217..21041)
                     /locus_tag="IV328_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8937416.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IV328_001266"
     CDS             complement(21052..22347)
                     /locus_tag="IV328_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8937417.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IV328_001267"
     CDS             complement(22363..22842)
                     /locus_tag="IV328_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8937418.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IV328_001268"
     CDS             complement(22848..23876)
                     /locus_tag="IV328_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8937419.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IV328_001269"
     CDS             complement(23893..24999)
                     /locus_tag="IV328_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8937420.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IV328_001270"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IV328_001270"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8937421.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IV328_001271"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IV328_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8937422.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IV328_001272"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IV328_001272"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8937423.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="IV328_001273"
     CDS             29970..30668
                     /locus_tag="IV328_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8937424.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="IV328_001274"
     CDS             complement(30836..33475)
                     /locus_tag="IV328_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8937425.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         9606 a         7460 c         6156 g        10841 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt ttt
//
LOCUS       DACDKJ010000023        33918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDKJ010000023 DACDKJ010000000
VERSION     DACDKJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.023"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            155..2053
                     /locus_tag="IV328_001275"
     CDS             155..2053
                     /locus_tag="IV328_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8937426.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2352..3071)
                     /locus_tag="IV328_001276"
     CDS             complement(2352..3071)
                     /locus_tag="IV328_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8937427.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3408..3740
                     /locus_tag="IV328_001277"
     CDS             3408..3740
                     /locus_tag="IV328_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937428.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3727..5730
                     /locus_tag="IV328_001278"
     CDS             3727..5730
                     /locus_tag="IV328_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8937429.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5749..6219
                     /locus_tag="IV328_001279"
     CDS             5749..6219
                     /locus_tag="IV328_001279"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8937430.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6240..6824
                     /locus_tag="IV328_001280"
     CDS             6240..6824
                     /locus_tag="IV328_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8937431.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6839..7825
                     /locus_tag="IV328_001281"
     CDS             6839..7825
                     /locus_tag="IV328_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8937432.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7815..8126
                     /locus_tag="IV328_001282"
     CDS             7815..8126
                     /locus_tag="IV328_001282"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8937433.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8148..9929
                     /locus_tag="IV328_001283"
     CDS             8148..9929
                     /locus_tag="IV328_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8937434.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9922..11298
                     /locus_tag="IV328_001284"
     CDS             9922..11298
                     /locus_tag="IV328_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8937435.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11305..11940
                     /locus_tag="IV328_001285"
     CDS             11305..11940
                     /locus_tag="IV328_001285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8937436.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11970..13325
                     /locus_tag="IV328_001286"
     CDS             11970..13325
                     /locus_tag="IV328_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8937437.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13517..14911)
                     /locus_tag="IV328_001287"
     CDS             complement(13517..14911)
                     /locus_tag="IV328_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8937438.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            15003..15974
                     /locus_tag="IV328_001288"
     CDS             15003..15974
                     /locus_tag="IV328_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8937439.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15971..16753
                     /locus_tag="IV328_001289"
     CDS             15971..16753
                     /locus_tag="IV328_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8937440.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16922..17293)
                     /locus_tag="IV328_001290"
     CDS             complement(16922..17293)
                     /locus_tag="IV328_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8937441.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17427..17870
                     /locus_tag="IV328_001291"
     CDS             17427..17870
                     /locus_tag="IV328_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8937442.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17913..18722)
                     /gene="yidA"
                     /locus_tag="IV328_001292"
     CDS             complement(17913..18722)
                     /gene="yidA"
                     /locus_tag="IV328_001292"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8937443.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18741..20108)
                     /locus_tag="IV328_001293"
     CDS             complement(18741..20108)
                     /locus_tag="IV328_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8937444.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20092..20922)
                     /locus_tag="IV328_001294"
     CDS             complement(20092..20922)
                     /locus_tag="IV328_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8937445.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21085..21522
                     /locus_tag="IV328_001295"
     CDS             21085..21522
                     /locus_tag="IV328_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8937446.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21598..22218
                     /locus_tag="IV328_001296"
     CDS             21598..22218
                     /locus_tag="IV328_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8937447.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22407..22913
                     /locus_tag="IV328_001297"
     CDS             22407..22913
                     /locus_tag="IV328_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8937448.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22930..23829
                     /locus_tag="IV328_001298"
     CDS             22930..23829
                     /locus_tag="IV328_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8937449.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23916..24800
                     /locus_tag="IV328_001299"
     CDS             23916..24800
                     /locus_tag="IV328_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8937450.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24992..25063)
                     /locus_tag="IV328_001300"
     tRNA            complement(24992..25063)
                     /locus_tag="IV328_001300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25028..25030),aa:Arg,seq:ccg)
     gene            25123..26457
                     /gene="rpoN"
                     /locus_tag="IV328_001301"
     CDS             25123..26457
                     /gene="rpoN"
                     /locus_tag="IV328_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8937451.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26660..27697
                     /locus_tag="IV328_001302"
     CDS             26660..27697
                     /locus_tag="IV328_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8937452.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            27738..28739
                     /gene="gap"
                     /locus_tag="IV328_001303"
     CDS             27738..28739
                     /gene="gap"
                     /locus_tag="IV328_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8937453.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28849..30039
                     /locus_tag="IV328_001304"
     CDS             28849..30039
                     /locus_tag="IV328_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8937454.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30143..30898
                     /locus_tag="IV328_001305"
     CDS             30143..30898
                     /locus_tag="IV328_001305"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8937455.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            31014..32312
                     /gene="eno"
                     /locus_tag="IV328_001306"
     CDS             31014..32312
                     /gene="eno"
                     /locus_tag="IV328_001306"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8937456.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            32711..33850
                     /locus_tag="IV328_001307"
     CDS             32711..33850
                     /locus_tag="IV328_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8937457.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        11208 a         6006 c         7217 g         9487 t
ORIGIN      
        1 aaaatataga aggaagctgg acatttattg ttcccagctt cttttttgta tagacctatt
       61 tctatttttt tattatattg gatatatata ttcattgaaa atagagcata acaactttat
      121 aatgttgagt ataccaattt ttggaggcgt tttaatggac cgctatttag atttactttc
      181 agagaagttc cctacaacgg aagatgtcat cacggaaatc atcaatctcg aagcaatttt
      241 gcagcttcca aaaggaacag agttattttt gagcgatata catggcgaat ttcctgcttt
      301 tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa aagatcaaag accttttctc
      361 tgaacaaatg acggaaacgg agatcagcca attcactatt tttacggctt atccagaata
      421 tgtgttgacg actgattggt acaaaaaaca agataaatcc cagctgatcc atcgtttgat
      481 cgatctttta cggtttacga cagtcaaata cactcgatca aaagttcgaa aaggattgcc
      541 gaaagaatat agctatatca ttgaggaact cttgtatatc gatgatcgta tcaacgggaa
      601 aaaagactat gtaaaaaaaa tcattgaaca acttgtcttg atgaaggaag aagaaaaatt
      661 tttaacaaaa cttgcgcaga cgatccaaaa atctgtcatc gatcatttgc atatcgtggg
      721 tgatattttt gatcgaggaa ctcaagcagc caaagtaatg gatcgaatca tggatttttc
      781 ctctgtagat atccagtggg ggaatcatga tattttgtgg attggtgcgt attgcggctc
      841 tgaggcatgt ttattgacgc ttttacggat tgccgcacgc tacaactatt tatttgaatt
      901 agagaaagaa tatggattaa atttacgtcc attattttct tttgcccatc aaacctatca
      961 gaagaatcca gtatttaccc ctaaaaacag gaaagatcta tctgaaagag agcaagaaga
     1021 attagaaaag atccatcaag cactttctat cctccaattc aaatcagaac atcagttgtt
     1081 gaatcgacga cctgaattta aaatggacga tcgtaaattg ttggaaaaaa tcaactatga
     1141 ggaatcaacg atcgttttag aaggtcagct ctatgagttg aaagggactt gttttcaaac
     1201 gatccagcca gatgatccta aaaaattctt ggaagaggaa caaaaagtga tcgatagttt
     1261 gatgtattca ttccagcact cgttaagaat gcaaaaacac atgacgtttt tgatcgaaaa
     1321 agggggaatg tatctcacga acaatcagca tgtactgttc catggctgta tacctgtaga
     1381 tgaaaagggg cagctcttag catttgaatt agaccaatct tatcgcggaa aagaactgct
     1441 gggatttttt gaaaaacaga taactgaaag cgcaaaagat ttgactgcaa atgaagattt
     1501 ggcgaccgat ttgatttggt atgcgtggag cgggccattt tcgcctttat tcggcaagag
     1561 taaaatggca acttttgagc gctattttct cacagagcct catacgcacg tcgaaaaaag
     1621 caatccctat tatcacttgc gagatgaaga gtggttcagt gaaaaaattt tggcagaatt
     1681 cgatgcaaaa gaagaaggtt cagcaatcat caatggacac acgccagtaa aagtaaaaaa
     1741 aggagagtca ccgatcaaag caaacggaaa agcatttgtt attgacggag gtctatccaa
     1801 agcttatcaa aaaacaaccg ggattgctgg ctactcatta ctatgcaatt cttacggttt
     1861 ccaaatcgtg acacattatc cgttcttgcc tattgaacaa caatttgcaa aaaaatctga
     1921 tcaaacgaat gtaaaaaaag tgatcgagca gcagctccca cgcaaactta atcggaatac
     1981 gacaaaagga atggaattac agcaacagat tactcaactc caaaggctgt tggattatcg
     2041 aaaggacgat tgagaaaaat gctagccaac attgctcaat agactaaaaa gcgactgcaa
     2101 caaaaggcgt gtcgcagtct ccttcggaat tagctcaaaa aacggtaaaa tattagaagc
     2161 tatttttctg tttatttctc taatactcag gtcagggcgc tgatttcacg acctcttcct
     2221 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccaac aaacatgaga
     2281 agctgttttt tgaaattgct ccttgtgact cagagttgaa cggcaggctc acgacctaat
     2341 cagaaaaacg attaaaattc attcacaaag aaatcgaata aacgctgttc agatgaatct
     2401 acacgatgag aaagttccgg atgccattgg acaccaagga ttttgtgcat attatcttta
     2461 ctttggactg cttcaatgag tccatcgttg gaaaaggcga tggcttttaa caaaggagaa
     2521 agatctttga ctgcctgatg atgataggaa ttcacaaagt atgaatcagg gagaaggtca
     2581 cttaataaac tgtccgaaac gattttgact tcgtgagttg caaactgcgg ggcagtgggc
     2641 tgttgttcgt gttttacgga ccattttgga tataatgaaa gatcttgata aagtgttccc
     2701 tccaacacga cattcaatag ttgaagtcct cggcagactg caaaaatcgg ttttctttgt
     2761 ttcaatgctt ccaagatcaa tgcctgttcg aattggtctc gttgaatatt tgtttccgtc
     2821 agttttggat gagggtcttc catgtaaagt tgcggactaa catcctgacc gcctgcaaga
     2881 atgagtttgt cgattttctc aattagttga ggcgctgaat ctggagacat gactggcaga
     2941 ataagaggta atccttgagc atctaaaact gcatccacaa atccctgcgg tgtatagctt
     3001 acttgatttc cttggaaagt atctgtcgct cgaatcagtt gattcccggc aatgccgatg
     3061 attggtttca tttttattcc ccctaaagta gactaattgt aacaaggatt taaaaaaatt
     3121 taaactatgt aagacttgcc cctatactta tctgttcttg ctgtgttact atctgtaaag
     3181 agaattttcg tgtgaaattc atgacgatag ttgaaaatat tgcttaattt gttataataa
     3241 aaagtaagca gaaataaaaa ggttgggcgt aagcttcaag gatctagttc ttcaaggggt
     3301 gagaaggaca ttttattgaa ggggcctgcc tatactaagt tatctttaga ggtacatact
     3361 ttcaatgaaa aaagaaagca tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt
     3421 tagaagcttt ggaacaaatc cgaaaagcag aagaagaaaa tgagaagcgt gctcgaaccc
     3481 tacggcagca gttgaagacc tatgcagaag aaaaagagca agaattggag ataatccaaa
     3541 cgaacagtcg gcaacagctg aaacagttgg tagaagaaaa agaacaattc gaaaagcaag
     3601 aattagaaaa agaaaaaatg aaattgaaag cagaagcaga caaggtgcaa gcaactttga
     3661 aagaccaata tcaaaagcat cagagtcaag caatcggtgc aatcattgaa agggtgaaag
     3721 aaacttatgg ccgtcactaa gatggaaaaa gtgaccctca tttcagataa aagaaatcag
     3781 gagaggattt tacaagcagt acaaggaatc caaaatgttg aaatccggga tctgtatcaa
     3841 aatagaacaa acaaccagtg ggtagaaaaa tattttccag ataccacgat tattgataaa
     3901 gaagcaaaat taagcgcttt aggcaatcgg ctgaaagaca tccaagaagc tatccaattc
     3961 atacaacacc acggcgatag ccgtcaaaag aaactccatt tgaaacgtca agaactttcc
     4021 ttacaagact tggaagcaaa ttattccgaa gaaaatttca tgaataaatt agaagaagtc
     4081 cttgatttaa aagaaaagtg ggaacagcta actgaaaaac gtgatcgttt agaagaagag
     4141 gaagaatggc ttcttcattg gcaacagctg gatattgctc caagtatgta tgcaagtaag
     4201 acgaccattt ttgagatggg gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa
     4261 ctaagtaagc ttgatgacgt ttatatggaa gaaatagatt atacgccaaa acaagtttat
     4321 ctttcgtatg ttgctttaaa aaaatcagtt gctgctatag aagaaattgc tgggaaatac
     4381 gggttcaata aagaaacgta tccttatgaa caaaatccta aagaaaaatt gcaagaaacc
     4441 aaacagcaat tatccattgt tcacgagcag caaaaaaaga taacgacagc gttaggtcag
     4501 tgcagcggct atatccaaga atttgaatgg gtagaagaag tcacacttgc cgttgcagaa
     4561 cgggaaaaag taaaagaacg gtttatccat gccacttatc tgatcgtttt gcaaggttgg
     4621 gtagatgcag aagaaaaaca gaatttgatg catgtgttaa acgaaactgt atcagaagaa
     4681 gatatatacg ttagtttcga aacaccaaac tcagaagaga ttcagcagga agtccctact
     4741 aaattgaaaa accatccgct tatcgaacct tttgaattgc tgactgaaat gtacagtctg
     4801 ccaaaatatg aagaagtaga tccaacgcct tggatgacac cattttatct tgtcttcttc
     4861 gggatgatgg tcgcagatgt tggttacgga ttgctgatgc tcattgggac aatactagct
     4921 caaaaactat ttgttttacc ccgcgggatg aaacgtttcg taaaattctt tgagattctc
     4981 tcggtaccat cgatcatctg gggattgatc tacagttcat ttttcggaca ggcattacca
     5041 aaagaactat ttggtatccg cttgcctttc ccaatcctat ctacgacgga tgacgtcaat
     5101 acgattttga ttttatccgt gattttcggt ctgatacaga tcttggttgg attattcgtg
     5161 gcagcaaagg aaaatatcag gaggaaagat tacttgagtg ctgtcagtga tggttttgcc
     5221 tggcaaggga tcctgattgg gatcgttatt gcattgattg gtgtcatgtt gataaaaaat
     5281 acagcatttg tttatcttgg cgcggggcta tcgatcattg ctgctttatg tatcttaatc
     5341 gtgccgatta tccaatcgcc gtctaaagtc aagggtgctg ccaaaggagc gtataatctt
     5401 tacggattga ctggttatat cggtgattta gtcagttata cacgtttgat ggcactaggt
     5461 atttcaggcg gaagtattgg cgcggcattc aatatgctag ttgctttcat gccgccagcc
     5521 gctcgattca gtgtagggat ccttttgatc atcgctttac acgcactgaa tatgtttttg
     5581 acactgttaa gtgcctatgt ccatggtgca cgattgcagt atgtcgaatt ttttggaaaa
     5641 ttttatacag gaggcggacg tgcctttgac ccgctgaaaa cggcggaaaa atacgtcaac
     5701 atcaatcata agaaaaaaaa caataaataa ttggaggatt tttatacaat gatggattac
     5761 ttaattagtc aaaatggtgg aatggtattt gcagtattag cgatggcaac agcgacgatt
     5821 ttttcaggga ttggttcagc taaaggtgtt ggaatgacag gtgaggcagc agcagcgtta
     5881 acaacaagtc aacccgaaaa attcggacag gctttgattc tgcaattgct tccaggtacg
     5941 caagggttgt atggattcgt tatcgccttt ttgattttta tcaacttagg aaatgatatg
     6001 tcagttgtac aaggattgaa ctttctaggc gcttctctgc cgattgcttt tacaggtttg
     6061 ttttcaggta tcgcccaagg gaaagttgca gcagcaggta tccaaatttt agcgaaaaaa
     6121 cctgaacatg ctacaaaagg aatcattttt gctgcgatgg ttgaaacata tgcgatttta
     6181 ggtttcgtta tttccttcct attagtattg aatgcctaag cataaaggag gaaaagcaga
     6241 tgaacgccat tgaaaaaatc atctctcaga tgaatgaagc agcagagcaa gaaagagcgg
     6301 cgttagagca agaagagcga atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaaa
     6361 tcgaaacaga acatcaaaaa caaaaagaaa aacaaatcga attacttgaa aaaaaatacc
     6421 gtcagcttag gaatcgccaa caagtagaag tacgacaaga aaatttgaat gcgaagcagg
     6481 aatttttacg acgattgttt gcagatgcaa tagctgaaat ggagaattgg gatgaatctg
     6541 aacaaatcca gtttatcaaa aattcattgt attcattacc tttgacaggc aaagtaacat
     6601 tcattgctgg agaaaaatca gcagcttatc tttctcagac attgctggat gaatggaaca
     6661 atgaacttcc ctttatgatg gttttaagtg atgaaactgt tgctgatcaa gcgggttttc
     6721 tgataaacga tcaaggagtt cagtataatt ttcttttcag cagccttgta caagatatcc
     6781 aaggaacgat gagttttgag attgccaatc aattattcga ataaggaggg atcagtgtat
     6841 ggaataccac gaactaaatc ccttgattcg cggaagagag cttgaacttt tgtccaatga
     6901 gacatttgaa cggatgattc aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc
     6961 cacgatttac agtccatata tttatgacgg atatgaatat gacttcgaac agaatctttt
     7021 aaaggagcag agccgtctat ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt
     7081 ttggatctat acgatgcgct atactttcca taatctgaag gttttgacaa aagccgagat
     7141 gacaaatcaa aatctggacc atttgtatat cgacgatgga ttttattcat tagaaacctt
     7201 gaaggatgcc attcatacgc aagtttcaaa tgaactccct gaaagcatta tggacagtat
     7261 acgagaagta cgtgattatt ttgaagagtc taccattctt cagggaattg acgtgattta
     7321 tgatcgacag tttttgacag agcagcgacg gcttgccgat aagctaggat atcaagaatt
     7381 gctgacagaa gtcattgctt ttattgactt gaccaatatc acaacgatgg ctcgaggaat
     7441 attgcagaaa agaacatatg ggttcatgac gacggtactt tccagctcgg gaagtatcga
     7501 aaaagaagta tttttgaact ttgtagataa agatatggaa gattacacga attttctatt
     7561 agagacagat tatgctgatt ttctgcgccc tgtcatccgt gagggaaatc ttgatctcgt
     7621 aaagcttgaa cagttgaaag atgattatct aagttcattg tatcaagaag cacaaaccca
     7681 ggctttcgga ccactgccac tactagcctt tttgaacgca aaagaggtag aaagcaaaaa
     7741 ccttcgttta ctggttatcg gcaaaagaag ccatttcgaa ccagaaacaa tcagaaagcg
     7801 ggtgagacaa gtctatgacg cataagatcg gtgtagttgg cgacaaagat tctgttttgc
     7861 cattcaaatt gtttggcttt gatgttcgtt atggtacaac caagcaaacc gttcgtcagg
     7921 caattgaaga aatggcaaag aatgagtatg gcgtgattta tgtcacagag caatgtgcag
     7981 cattgagccc agaaacgatc gaacgttaca aggaacaaat gatacctgct atcgtattga
     8041 ttcccaatca tcaaggatca ttagggatag ggctggaaga aattcaaaaa aacgtagaaa
     8101 aagcagtagg tcaaaatatc ttatagtatg aaaaggagat agaaattttg caaattggaa
     8161 aaatcataaa agtctccgga cctcttgtaa tggctgagaa catgtctgat gccagtatcc
     8221 aagacatgtg tctagtggga gatctaggag ttatcggaga aattatcgag atgcgtggcg
     8281 atgtggcgtc tattcaagta tatgaagaga cttctggaat tggtccaggc gaaccggttc
     8341 gctctactgg tgaagcttta tctgttgaat taggaccagg gatcatttca caaatgtttg
     8401 acggaatcca acggccatta gataccttta tggaaatcac acaaagtaat ttcttagggc
     8461 gcggagttca attgcccgca ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag
     8521 aagccggaga aacggtgagt gctggagatg ttatcgggat cgtagaagaa acaaaagtta
     8581 taaaacataa aatcatggtg ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag
     8641 gttcattcac tattgatgaa acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt
     8701 tgacgatgct tcaaaaatgg ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc
     8761 cggatgcacc catgatcact ggacagcgag tgattgatac attcttccca gtgacaaaag
     8821 gaggagcagc agctgtacct ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa
     8881 ttgcaaaatg ggccgatgtg gacatggttg tctatgtggg atgtggcgag cgtggaaatg
     8941 agatgacgga tgttttaaat gaattccctg aattgattga ccctaataca ggagaatcgt
     9001 tgatggaacg tacggtattg attgctaata catcaaacat gccagtagca gctcgagaag
     9061 cttctatcta tacgggaatc acaattgcgg aatatttccg tgacatgggt tatgacgttg
     9121 ctatcatggc agactccact tcacgatggg cagaagcact tcgtgagatg agcggacgtt
     9181 tggaagaaat gcctggtgat gaaggttatc cagcttatct tggttcacgg cttgcggaat
     9241 actatgaacg atctggaaga gtcattgcat taggatcaga aggtcgcgaa ggaagtataa
     9301 ccgcaatcag tgctgtatcc ccgtctggag gggatatttc ggaaccagtc acacaaaata
     9361 cattgcgggt cgtaaaagta ttctggggtc ttgattccag tttagcccaa aaaagacatt
     9421 ttccttcgat cgattggata caaagctatt ctctttattc aacagaagta gggaagtata
     9481 tggatcatat ccttcaagct gactgggcat ccatggtcac cgagggcatg cgtattttgc
     9541 aggaagaaca gcagctgaat gaaatcgtgc gtttggtagg gattgattcc ctttcagata
     9601 atgaccgtct gacattagaa gtggcaaaat ccattcgtga agattatttg cagcaaaatg
     9661 cttttgatga tgtcgataca ttcacttcaa gagagaaaca attcaaaatg ttgaagatga
     9721 tcttgacctt tggtgaagaa ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga
     9781 tcatggacgg taccgtatcg attcgtgaaa ggatcggacg aagcaaatat atcccggaaa
     9841 cagaattaga aaaactagat caaattattg gtgaaatcaa gcaaacgatg caaacgatcg
     9901 tttcagaagg aggcatgagc aatgattaaa gaatatcgta cgatcggtga agtcgtcggt
     9961 ccattgatgg cggttgaaaa agttgctggt gtcaaatatg aagaattaat cgaagtccgt
    10021 atgcaaaatg gcgagattcg tcgtggacaa gtattggaag tccaagaaga taaagcaatg
    10081 gtccaaatat tcgaaggaac aagtggtatc aatttacgtg actcttctgt acgattttta
    10141 ggccatcctt tagaactagg tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg
    10201 ggacgaccga aagataacgg accagagatc ctgcctgaaa agcatttgga tattaacgga
    10261 gaagtaatca atccagtagc tcgggattat ccagatgagt ttatccaaac aggtatctct
    10321 gcaatcgatc atttgaatac attagttcgt gggcaaaaat taccagtatt ttctggctct
    10381 ggtcttcctc ataaagaatt agctgctcag atcgcgcgac aagcaacggt actcgattca
    10441 tcagatgatt tcgccgtagt ctttgctgct attggaatca cttttgaaga agctgaattt
    10501 ttcatggaag attttcgtca aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta
    10561 gcaaacgacc ctgcaatcga acgtatcgct actcctcgta tggctttgac tgctgcagaa
    10621 tatcttgctt atgaaaaggg aatgcatgtt ttggttatca tgacagacat gaccaactat
    10681 gcagaggcat tgcgagagat ttctgcagca cggcgagaag ttccaggaag aagaggatat
    10741 cctgggtatc tttacacgaa cttagctact ttattcgaac gagcaggaag aatccgcgga
    10801 ctaaaaggat cggttactca aattccgatt ctaacaatgc cagaagacga taaaactcat
    10861 ccaatccctg acttgactgg ttatattaca gaaggacaga tcatcttgac tagagagctt
    10921 tacaagagtg ggattcagcc accaattgat gtactcccgt ctctttcgcg gttaaaagat
    10981 aagggaactg gacccggaaa gacaagagaa gaccatgcag ctacgatgaa tcagcttttt
    11041 gcagcctatg cacaaggaaa acaggcaaaa gaattagccg tagttttagg tgaatctgct
    11101 ttatctgatg tagacaagat ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc
    11161 aatcaaggat tctacacgaa ccgttcgatt actgaaacac ttgatctagg gtgggaactg
    11221 ctatcgatgt tgccaagaac cgaattaaaa cgaatcaaag acgacatgct ggacaaatat
    11281 ctgccagaag ggaagtgagt tctgatggca cgattgaatg tgaatccgac ccgcatggaa
    11341 ttgactcgat tgaaaaaaca gttgacaact gccacaagag ggcataaatt attaaaagat
    11401 aagcaagatg aactgatgcg tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt
    11461 caagaaatgg aagagtctat gacagaagca atgtctgatt ttgtattggc aaatgcttcg
    11521 atcacggaac ctttcatcga agaattattt gttttgccgg cggaaaacgt tgatttgtct
    11581 atcgtagaca aaaatatcat gagtgtaaaa gtacctgtga tgaattttga atatgatgaa
    11641 cagctgacag acacaccttt ggaatacggg tatttgaatt ccagtgccga actggatcgg
    11701 tcaatcgatc ggtttactac cctgcttcca aaacttttgg aactgacaga aatcgaaaaa
    11761 acttgtcaac tgatggcgag tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa
    11821 tatatgacga tccctcaatt agaagaaacg atctattata ttcgtatgaa gctggaagaa
    11881 aacgagcgcg cagaagtaac aaggttgatc aaagtaaaaa atatgggtac cactgactaa
    11941 gtggtatctt tttttaaaag aggaaaacga tgattaaaca aagcaaacaa ataaagaaac
    12001 gtttgccggc tgttcagctt ctcgcattag gattttttct cctgatttta gttggcggcg
    12061 gcctacttac cctaccattt tttagtaata gtgggcatgg gacaaatttt atcgatgcgc
    12121 ttttcactgc aacttctgct gtctgtgtaa cgggattgac aacattgaat acagcagagc
    12181 attggaacag tgctggtcaa tttttgatca tgcttttgat tgagatcggc ggtcttggtt
    12241 ttatgatggt tccgattcta tgctttgcat tgataaagaa gaaagtcagt ctcagcacac
    12301 gtattatatt aaaagaagcg ctgaatctag aagaagtatc aggtgtgatg aagctcatgc
    12361 tttacatcct aaaattggcc tttgttatcc aattgattgg tacggttgct ttatcttttg
    12421 tatttgtgcc tgaatttggc tggttgaaag gtatctggta cagtttgttc catgcaatct
    12481 ctagtttttg taatgcaggg tttgatttgt taggagacag tttagccaat caccagcaaa
    12541 atgtttattt gatcatggtg gtatctgctt tgatcatttc tggtgggtta ggcttcatcg
    12601 tatggcgtga tttactggaa tatcatcaga aaaagaagat gacgattcat tcaaaaatcg
    12661 cattaaccgt tacaggtacc cttttattag gcggattcgt tgtgttcttt ttcacagagc
    12721 gcaatggggc aacgctagtg gaagggaata tgtttcaacg attggcaaac acctttttta
    12781 tgagtgtgac acctagaacg gctggatatt attcagttga ttacctccat atgagccatg
    12841 caggtctgat tttaacgatg tttttgatgt acatcggcgg aacatctggg tcgacggccg
    12901 gcggattgaa aacaactacg ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag
    12961 gacgtacgag agcggaagca tttggccgta ccatccgaca aggcgcagtg ttgcgggcac
    13021 ttactttatt ttttgttacg ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga
    13081 ctgaaacgat acctaaaact tctggcatcg aatatattgc ttttgaggtc ttctctgcgt
    13141 ttggtacagt aggtcttacg atgggattga cgccagactt gactgcaata gggaaattga
    13201 tcattatagc gcttatgtac attggacgag ttggtgtatt gactgtggtc ttctctctga
    13261 tgacaaaagc acaaagaaca caatcaaaat acaaatatcc agaagaaagt gtcatgattg
    13321 gataatttca tcttctgaca gaaacctagt gatttagtaa aataagaaaa taaaataatt
    13381 tttcttgctt cttggatact ttaagtgcaa aatgttcgtt tattttttga ggcactaccc
    13441 gttgttgaac ctcgggtagt gcctcttttt tattgaaagg aatcaataag atttgattcc
    13501 tttatttatc aataggttat aattcatatt tttttatttt ttcttttacg atacttagtc
    13561 cagaagctaa aatttcaagg ttattagagg atagggaaat tcgaacgaat gcatctggtt
    13621 gcgcacgtgt agtaaagcgt ctggaatgat aaagtcggat accgttagca agaaagtcat
    13681 tctcaattat cttagggtca atctcaggat caactggaag aattcgatag taagggacac
    13741 gattcgttgg tttttctact ttgaaaagag aatcaaaaag ctgattggct ctttttgtaa
    13801 gacttaattt agtttctaag atcctatttg cttcatttga atggaggatt tgagtgatga
    13861 cttccacatc taatccagaa gttttcacat ttatattaaa aattgcttgt tctatcttgc
    13921 tttggacatg tactggaact acaagataag caactcgcaa tccagaacaa ataaattttg
    13981 tcattccagc taagtagatt gtttggttag gtaataaatt ttggaaagga ggaatgatcg
    14041 tatgttgata gtaagttgtt aaaaatgaat ggatatcatc ttcaataacc catagacctt
    14101 ctttttcaat cacttcggct aattgctctc gtcttttgtg agagatttga aaccctatag
    14161 gattattaca agaaggcata agaaagacac catgaatctt cttttttcta cattcctgaa
    14221 tcaatatatc aggtcgcatt ccttgctcgt caaaatcaat tggaacgatc tctaaatgat
    14281 ataactgagc caattcaatg aaattagcgt atgtatatcg gtctgttgct attctatcac
    14341 ctggagagaa aagtgcagaa agtgtgatag ccaatccatt ttgaacaccg gaaacaatag
    14401 atagagattc taaggacgga atgactcctt gagtttttaa ccaatttgct gcagtagtca
    14461 attgaaaatt tgtgcctttg ggatcttggt aattaagcaa accaacaaga tttgggttat
    14521 tactagttga ttgaataaac gggacaacga atttattaca ttcttcaaaa gaactaacca
    14581 gaccaaaatc aatgatatgt tcggttactt cattggtaga gatggtgatg gactcaaaga
    14641 cattgggaga aataaatgtg ccacttccaa tattcgtata cagcaaacct ttctcaattc
    14701 catatttata agccttgcca acggtagtaa aatttaaatc taaataatca gctagctctc
    14761 tttgggaggg taactgagtg tttttctgta atttattctt taaaatatct tgttctaatt
    14821 gttgaatcaa cgatcgataa taaggacgct tcaatttttg tttgtctggt cgccaagtta
    14881 acgggtaatt gtcataagaa ttaataggca ttttctccac tccttaaaat tgtataccat
    14941 acaattttac cattgattgt attgatttta gaagttaatc ttttgtgagg agggaattgt
    15001 ctatgaaaat tattaatttt gaggaagtag tagcttcttt tagttttgaa gaagcgattc
    15061 aagtaatgaa aaaatgtttt tttgattatg aaagtggtaa aatcagccaa aatcctcgat
    15121 tggtggaaat tttacctgac ggaaaaggca aaaatatgtt tgcgatgatg cctgcttatt
    15181 tgggggagaa cagatatttt ggcacaaaag tcattaccgc ttttccggat aatcatcagt
    15241 atcatttacc ttctcattta ggagagattt tcttgtttga tgctcaaaac ggactaccca
    15301 aagcattgat aaacgcaaat gcagtcacct ggattcgaac agctgctgta tctgcgctgg
    15361 caacagatta cttagcaaat ccgcatgcag aaacacttgc tttaattgga gctggtcaac
    15421 aagcattgtc gcatttgact gcgatttcta ccattcgtcc catcactaca gtgtttgttt
    15481 acgatatttt agaagaaaga cgaaatactt ttatcgctaa agctaaaaaa cagtatccta
    15541 aaataacgtt tataaatgca atatcagtag aagatgcagt acgtccagcg gaaatcattt
    15601 gtacgttgac ctctagtaaa gaggcatttc ttcaaaaaga gtgggtaagg gaagacgcac
    15661 atatcaatgc tgtgggcaca tttactcctg atacaagaga gataagtaca tcgttgatgg
    15721 aaacttctca aatctatgta gatgattact cagctgcctt aacagaaagc ggggacctgc
    15781 ttatagccat ggcagaagga gatttagagg aatcttgtat tgttggtaca ttaggcgaat
    15841 tagtttctga gaaaaaaaca ttttataaag gagaatcgag gccgacgatt ttcgacgcag
    15901 ttggtctagc tattgaagat ctttgttgtg cagaatatat ttataacaaa ttagagaaag
    15961 gggctttaaa atgaacttag attattacgt agttgatgca tttgcagatg aggtgttcaa
    16021 aggaaatcca gcagcggttt atgtgctaga agaatggctg ccagaaggta cgatgcaaaa
    16081 aattgccatt gaaaacaatt tatctgaaac agcatttaca gtgaagaaaa accaagaatt
    16141 tgagcttaga tggtttacgc cagatcgaga aatcgatctt tgtggtcacg cgacattagc
    16201 aacagcattt gttttattta actattacaa aatacctgat gaaacgatta aattttctac
    16261 tcaaagcggt aatctatttg tgacaagaat taaagattat tattacatgg attttcctag
    16321 tattatgccg aaaaaagtgc cgatattgtc tgaatatgaa gaagcgattg gggctaagat
    16381 taaagaagcc tacttagcac gtgatttatt tttcgtatta gaagatgaaa aaacggtggc
    16441 taaattgcag ccagatttta cagctatcaa aaattttgaa ttaggaatag gagtcatcgt
    16501 gacagcagaa agtgtgcaaa aagactttgt ttctagaacc ttctttccaa aattaacaat
    16561 caatgaagat ccagtatgtg gatcagcaca ttctaatctt attccttatt gggcagaaag
    16621 aatgaataaa acaaaattgt cagcttatca aatgtcatca aggggcgggg atctagaatg
    16681 tgaattaaaa gaagatcgtg tgataatagg aggaaaagca gttttgtttt ctaaaggtac
    16741 ctcgtttatt tgatttcttt caaatgaatt aaaaataaat tggtggagag tggcaacctt
    16801 atagctacaa tggttgttaa aaaaagacgc aaactttttc aatttaatga aaagtttgcg
    16861 tctcatttta attattggtg tttgtttttt attcaatcaa gagtaaaatg ccccatacta
    16921 actatcctcg gatgacttcg attttataac catcagggtc aatgacaaag tagtaagaag
    16981 gcgcagtgcc aggtaatcct tttaagtcag taatctcaag accggcagct ttatgttgct
    17041 cgtgtaattt ttctagatca tcagctgcta tagcgatatg accatagccg tctcctagat
    17101 catacgcacc atgatcatag ttgtaagtca actctaattc atagtcatcc ccaggaagtg
    17161 tcaaataaac aagtgtaaat ttgttttctg gaaaatcacg gcggcggctt tcttcaaaac
    17221 caaatgcctt ttgataaaat tcgattgatg cttcaagatc tttcactcga acacatgtgt
    17281 gagccatttt catagattca tctctccttt tccttttact caatcataac aaaaagaagt
    17341 aaggaaatag ttgatttgtg cttgttcatg agaaatttct catgtaaaat ggatagaaaa
    17401 taaaactgtt aaggtggaaa ctattcatgg ataaaccaat ttttggtgaa aaaaagcaat
    17461 ttgctgatta tcaaacccgt tatgcagcat atattgtcat agaaagagat cagcagattg
    17521 cagtaatcga ggctccaaat ggtgcttttt ttcttcctgg cggggaaatt gaaggagaag
    17581 agacgaaaga agaagcgatt gtccgtgagc tgatagaaga aatgggaatt gtggcagaaa
    17641 tttcttctta tctgggagaa gctgatgagt atttttactc gaattatcgt gcaacttatt
    17701 attataatcc aggatatttc tttgttgctg attcttggaa gcgtatagga gaaccaactg
    17761 aaaagaccaa taaaatttgg tgggtgagcc ctcaagaagc gcttgtgaaa ttgaaacgtg
    17821 gtagccaccg atgggcagtc gagagatgga tcgaaaaaaa taaaaactga aagaaagcga
    17881 gtagatcagc tttctctcag tttttttatt tattacagta catatttttt gattgcttca
    17941 gctacaccgt gctcgtcgtt tgatgaagtg atgacatttg ctgcttcttt gacaagagaa
    18001 acggcatttc ccattgctac tcctagacca gcatattcaa tcatggataa gtcgttctcg
    18061 ttatctccga tagccatgat ttcatcttga tcaataccaa ggtgctgtgc gagattcgca
    18121 actgccgcac ctttacttgc ttctttattt agtacttcga agtaataggg cgcacttttg
    18181 accgttgtgt atttatcacg gaaagtttgc ggtaaacgcg caattgctgc atctaaaatt
    18241 tctggttcgt cgatcatcat cattttgacg atgttcatgt ctggtgtcat ttcttcaggt
    18301 gttcgatatt ttagcggcat cttgactaaa cttgcttcgt ggatcgtata agcgctgata
    18361 tctcgattag gcgtgtagat cgctttgtca tcgatcgaat ggagatgact gccgacacgg
    18421 cgtgccatca cttcgatctc taggaaatct tcatgtgtca gtccgtgacg ggcgatgatt
    18481 ttacctgatt tcgtttgttg tacgagtgcc ccgttataag taataacgta atcattatct
    18541 tgaaaaaggt tcaactcgtt taactgttcc tggacacctg gtaagggacg gccagtgcat
    18601 aaaacgatcg aaacaccttt tgcaacagca gcggcaatcg cttcttttac ttcttttgtt
    18661 acttctcttt tctcatttaa taaagtacca tcgatgtcga tagcgactag tttgatagac
    18721 ataatttcct ccaatatatt ttaatttatt tcaatcaatg cgccattacg gatatggctg
    18781 gcaaattctt gataagtttc gtcaaataaa tcataatgat cacgcaagga cggatcaacc
    18841 atttcttttg gaaagaaaaa tcgctcatct ccttgttctt gtcccgctaa tgcagcaaca
    18901 agctgactaa cctgtgataa ttctatcaaa gttccgtcat ttcgcaaaat ctcgatttgt
    18961 gtgcgatgtc tgccttgttc tgggcgataa aaatcataag gtagatcgta gctagaattc
    19021 acagcagtgt aataaacagg attatatccg accttttcta ctaacaaagt gagttcttgg
    19081 actaattttg aatcccggtt gccagaaaaa gtagcagatt tcagtggttt acgattcaaa
    19141 aaacgtttag ctaaatcatt taaaatagga tcagcagatt cagaccattg agtaaagtaa
    19201 gtgcccaaga ccccatcatc aagtttgagg tattcttcta aagtaaagct tccgtcaaga
    19261 aatggcagaa gtaattgaga atggagttca aaactgctgg catcttcttg atacaactcg
    19321 tgtgcgcgat gaagcagatg ctccaaaatt acttccatac cacgggaaac aggatggaag
    19381 tacacttgca catacatttg atatcggcta acgatataat cttccactgc atgcatgcca
    19441 ctcatggaaa aagcgatccc gccttcataa ggacgaatca cacgtaatat tcgggtaaga
    19501 tcaaatgttc catattctgt tccagtgaaa taagcatccc gaagcaaata gtccattcga
    19561 tctgcatcga tctgactaga aatcatttgg acaacttgtg ggttggggta agttttttgg
    19621 atcacactag ctaccttttc aggaaaacct tcttctactc ggttaagaat ctgatacact
    19681 tctgtttctg gagaagtgat gatctctaca gtgatggctt catgattcgt atggaagata
    19741 tgttcaaatg tatgagagta aggaccatga ccgacatcat gcaacagtgc cgcacataaa
    19801 gtcaccagcc gttcctgatc atcccagcct ccgttgccta ttttttcttt tgaaaaattt
    19861 cgagaaaaga tatcgcagat tcgtcttgaa atctcataaa cgcctaatga atgagtaaaa
    19921 cggctatgtt ctgctccgtg aaaagtgaaa gaagaagtgc ctaattgttt aatgcgtcgt
    19981 agacgttgga cttctttcga attgatcaaa tctaagatta cttgatgctg gacatggata
    20041 tagttatgga ctggatcacg aaaaactttt tccataggca gcatttgata tttataaggg
    20101 gttgtcataa tgtagctcct ttatgttatt acgttgttcc attctctgga tttggttgtt
    20161 ccattgctcc agctggattt ctttttttag ccagtcggta gaatcctgta aatcacttgc
    20221 agaatacagc ttttcaaaag cttttgcaaa tgcttctttg acttctttta caaaaagggg
    20281 ttggtcaagc agttcttcaa gtgttgccat tgtagcagga tccactgcag gatagccatt
    20341 tgtaccaaag tcttctttta aagcagattg ataaaatcgc ttcatgactt gcccgcgttt
    20401 ttcctgatta ccgttgacac ttaagtacat cataacagaa atcccatttt tgatccgacg
    20461 ctgagcgata ccggcaaatt ttttgccatg gatgcttaaa tcataagttc caggacaata
    20521 agaatgggtg atttcgaatg cttgtatcgg tgcctttgga aaagcgtatt tagtcaatag
    20581 gagcatttgc tcgtaagcgt catctatggt tatttttttt gtgtcacttt gggggaaaat
    20641 caaagaaata ttcaaaattc cttcatctga tacgatccct aatccacctg aatttctgat
    20701 cactggagcg taacctgcat ctttcagtgt ttggatcccg tcacttagaa aaggagttcg
    20761 aacatccttc atacctaaga tgactgtgca gtcatattgc cagaaatgta aaaagatttg
    20821 ctggtgttct ttagaatatt ccgttaatat atctgttaat gcaaaagggg aaaagtcgtt
    20881 tttttgataa atgtgctgat ctagtcgaat cgcagggttc attcaaccgg ctcctttgta
    20941 taaattcata ttttctacaa tattatactc taaaatcaaa gagttgtttt ggaaaaaagg
    21001 ttttctgtaa aaatggacaa gttggttgac aaaacaaaag aaactaggaa caatgtagct
    21061 atgagaagta aaggagaatg aataatggaa ttatcacaag gaacaccgat ttcaatcaaa
    21121 ttgcgtacaa aagtaaaaca gcaaggggaa gtgcaagatt tttattttga tttgaaaggg
    21181 caaatggtga caattggcga tacgctgtac atacgttata aagagattca ggaggagacg
    21241 ggcgatgaga ttcctgtaac gatcaaatta gttccagatg gtcatgtaca gctgatccgt
    21301 gcaggagaaa tgcggatgcg tttgaaattt ggctataaag aacggttaga aacatcctat
    21361 cgaacaccat acggtatgat ccagattgct acttttacga aagaactgca tgttagtttg
    21421 aaagaccgtc ccacaggagg gaaggttcga atcgattatg atctgttcat gggaccggaa
    21481 aaaatcggcg aatattattt gaccttggat tttacagcgt aaatataaaa gaaaagtttg
    21541 attttttgtg tctgattttg tatgataagg agtagactgt gaaaggacgt gtcctgattg
    21601 gaaattagtg tatttgaagg tgcaaacaaa agcgaattat caatgatcga agtagcacac
    21661 gcgatcttag aacaacgtgg cgatgtcatg gatttttctg atttagctaa ccagatccaa
    21721 aactatcttg ggaaatcaga cagtgaaatt cgtgattcac tcgcacaatt ttatactgac
    21781 ttgaatattg acggcagctt tatctcatta ggggataatc gttggggctt gcgttcatgg
    21841 tatgctatcg attcgatcga tgaagaagta aaccatggaa tggacgaaga ggatgaagat
    21901 actccacgtc gccgtaaacg caagaaagtc aacgccttta tcaatgatga tgaagacgcg
    21961 atcgattaca atgacgatga cccagaagat acagatctga cagaagaaga tgacgatgat
    22021 ctgtttgatg acgatgatga tgaagatgaa gaaatcgctg cttataattc tgacttgcaa
    22081 gaaatcggag cagatgaccc gacagatgat gaagaagact tgccaggaaa tatcgaagaa
    22141 gatttaagta tcatagacga agacgatgat gatgaagatt atgaagagga agaattttct
    22201 gactctgaaa aagaataaac attattttct tacgagaggt tgtgacagag acgatcagcc
    22261 tatcagaata aggaggtgtg acacgatttt tgtcacacct tcttttttgt atataaaaat
    22321 cccaagtaaa tacataaaaa ataaaaaaat tgaaactttt agcgcacctt gtacgtcata
    22381 ttaagtgagg cagaaaggag tggagaatga tttttactgg atataagaaa aaacggcaga
    22441 tcgaaaaata ccgtcacctg ctgaatggac tcatcgaaac tgattatcaa aaaatgttcc
    22501 gtatcacgat gaattacgta catgataaag aagaggcctt agatgtgatg caagatagtt
    22561 ttcataaagc cctgaactat tttgaaaaag aaaaagagat cgagcatttt agtgcatggt
    22621 tttatcggat attgatccgc acggcattag atagttggcg cagacaaaaa aggacgcctg
    22681 tggacttatt tgaaattcaa gatatccccc aacagttacc tttagaaact tccgttgcag
    22741 agctccatat gattttagat aaaatagaca gtccggaaaa agagattctg atcctctact
    22801 tttttgaagg atttcgtttg aaagaaattg cgactatctt ggatatgaat gaaaacacag
    22861 ttaaaacgaa aatgtaccgc tcattgaatg ctttacgtca aatcctagaa tgaaggagag
    22921 aagagtgcca tgtattccca aaaagaaaat ttaaaaaaac taaagaaaac ttataaagaa
    22981 caagcaattc ccaaagggct taaagaggag atccgccagc gttttattat cgaagagcaa
    23041 tcatttataa aagcaaagcg gagaaagaga cgtctccaag ggttcggagt atctttggct
    23101 atcgtaatga ttacaacaag tagccttctt tttaatagtc aagttcgaag ctttgcagaa
    23161 gatttaccca tcttaggatc agtcattgaa ttgatcttag gcgaacgctt tactgatcga
    23221 tctgaaaaaa tcgatattca ggtaccgaag atcaatacac aaaatgagaa agaaaataaa
    23281 acaattcatg gattaaatca aaaatacttt cgcgaaggac aagcagattt tgaaaaagct
    23341 gaaaaagaat atggaaattt tgaaacggat cattaccaag ttttgggaga ttatcagaaa
    23401 atcgtagacg ataaccggtt tctcgtgatt gaaaggcaga tcacccaaac agcagcagat
    23461 agccatgttg aaaaaagata cgacacaatc gacaagaaaa acagtgtcca gctctcactt
    23521 ccacttttat tcaaagatga cacctatcta tctgttttga caaaagaagt caaacggcag
    23581 atggccgaac aagtcaagga agatccatct aagtactatt ggactgagca agatattcaa
    23641 gaagggacga tagaaaaacc aacacttgtt acgcctacac gaagttttta tttgaacaaa
    23701 gagcatcaat tagttttgac tttttctcaa tacgaaattg ctcctggtta tatgggtaca
    23761 ccagaatttg tcattcctaa gtccgtgaca aaaacgattt tggcatcgga agattatttg
    23821 gatcattaga cgagaagtag ttgcgactag cagtttcaat tttcaagtaa gtacaagtaa
    23881 ataataagaa gagaaaaaga gaaagaagag atatagtgaa aagaaattat ttagttatca
    23941 gtattattgc tttatccgtg cttttgtttt tcgaaattac ttcggtggcc tccttgatca
    24001 ccgaagtaaa aggacaaaca cactcttcgg agcattcgtc acaatctttc aaacaatcag
    24061 aacaatcaac cattaaaagc caatcaactg aaacagctaa cgtcaaagaa gccattcaag
    24121 aatcagcgga aaaggtacaa agtcagtcat cacaatcaac acaagacagt acaaaaacga
    24181 gccgagatcc ggctgtcaaa agcgtcgcaa tcagctttga tgatggacca ggagcgacca
    24241 caacaccgca gcttctgcga atcttaaaag aaaaaaacgt gcatgccact tttttcgtct
    24301 taggagaaaa tacggcgcag catcctgaaa tcgtgaagca gacagctgaa gcagggcacg
    24361 agattggcaa ttacacatac gatcatcaga atctggccac tctttctgcc cagtcgatga
    24421 cggaggaagt gacgaaagca gacactgaga tcaaaaaagt cactggcaaa acccccactt
    24481 ttgttcgtcc gccctatggt tctgtcacga atgtaggagc aacgatcatc cagcgaccga
    24541 tcatcgaatg gtctgttgat tcagaagatt ggaagacaag aaatcctgat ttgattttgc
    24601 agaagatcca agcaacagta tacgatgggg caattatttt attccatgat atctatcctg
    24661 aaacgattcg tgccgttcct caagtgatcg attatctgaa agagcaaggg tatcgcatca
    24721 cgacagttgg ggatttatta gggcatccaa cggctgtcga gaactattat ggcagaaacg
    24781 atcatcggcc agttcagtaa gtgagacaag tatctttaat aaataacaca ttttaataag
    24841 tagttgaaca cataactacc ttacttgcaa aggcttaaca atatctatac aggtacaaca
    24901 aaaaactgtc caccaatttt ctttggcgga cagttttcac ttaaaaataa agaagcaact
    24961 attcgtaaat tatgctaaaa tagcagtaga acgcgtcctg agggaatcga acccccgtct
    25021 caagaaccgg aatcttacgt gatatccact acactaagga cgcaagcact gggataattg
    25081 taactaaaca gataaaaaat tacaagggga ttggacaaaa aaatgaaatt tgagcaaaat
    25141 ttttcacaaa aacagcagca gacacagaaa ctagccatga cgcaacagtt gcaacagtcc
    25201 atccaagttt tacaatataa cagtgaggag ctgttggcat tcgttgagaa tcaagcaatg
    25261 gaaaatccat tggtggaagt tgttgagccg gaatggcagc cagattacat aaaagcttct
    25321 tcatcttctt atgaaggaga agagacaaat tacctaaatc agattcctga tacaaaaggg
    25381 tctttatttg attcattgat cgaacaagtc catttgaatt accgagatac ttttttgagg
    25441 aaaatcgttc tttatcttgt agaatacatc gatttgaacg gatttttgac gattagttta
    25501 gaagaagccg gaaaagagat tggagctacg cctatccaaa tgctcgatgc gttgactctg
    25561 attcagcaat tagaaccagc tggagtaggt gcacgtagtt tgcaagaatg tctcatgctt
    25621 caaacagaac gagatgatta tgcgccggaa ttagcttata tcgtgttgga agaatgtttt
    25681 gaggaactgg tggagcgaaa atggaaagaa atcgcacaac gtttttctgt tgatctacat
    25741 gctgttcagc agatattcga ctatttacaa acgttgagtc caagccctgg ccggattttc
    25801 gaccgtagca gcgaattgta cattattcct gatgtccggg tattagtgga cgacgataaa
    25861 aatgtacaag ttatctcaaa ccgaaaaaat cagccaaata tacgctttca agagagttac
    25921 ttcaaacaaa tgtcacaaca agcagataaa gaaactgaaa attatttaaa agaacggaaa
    25981 caggaattcg aatggttgaa gaaaacgatc ctacaacgtg gggatacaat tttgcgggtt
    26041 gctcaagtca ttgtttcccg gcaaaaagct ttctttatcg acaaggaacg tccaatcaag
    26101 cctcttacgt taaaggaagt tgcaactgag atagatgtgc atgaatccac ggtcagtcgt
    26161 gcagtcaacg ggaaatattt ggaaacagat ttcggtgtat ttgaattaaa aaaatttttc
    26221 actacacgaa ttcctacgaa tagtacagaa cagacagaag acctttcagc agatacagcg
    26281 aagaaaaagc tgcaggaatt agtcgatcaa gaagacaaaa acaagccgtt gtcggaccag
    26341 aaattggttg aattgctgaa aaaagatgag atagcgatct caagacgaac agtagcaaaa
    26401 tatcgtgatt tattaggaat cccgagttca tcaaaaagaa aacgttatga taactaatca
    26461 gtcaaaagac tagaaaaagt atttcacttt ttctagtctt tttagttgaa aaacgagagc
    26521 gaaactgtta gacttacagt cgtgaggttg agatttccct ttattgatgt gaaattcgaa
    26581 tagttaattt ctttttttat cgtatatggg tcgttttaag tctacattgg acgtaaagtg
    26641 tccaactaag gagatcgcta tgctggatga aataaaaatg attgaatctg ttgcaccaga
    26701 tattatcgaa gtgttgcaag aacgctataa aattttacga aatatctatt ggatgcagcc
    26761 gattgggcgt cgaagtttat cggaaagtat ggggatcaca gaacgagtct tacgaacaga
    26821 aacagacctt ttgaaaaatc tgaatttgat cgacacctca aaaagcggta tgacgctgac
    26881 aactaagggc gaagaagttt atcaaagcct tgaaaacttc atggatcagc ttttaggtac
    26941 gcatcaaacc gaacaacagt tggcagaata ttttggtaac caacgctgta tcgttgtttc
    27001 aggaaacagc gaagagcaga ccaaagtggc tgatgcattc ggcgaagcgc tgagcgaagc
    27061 cttagaccga ctacttccag aaggcgagaa tatcatcgct gtgatgggag gaactactat
    27121 ggctgtggta gccgaacaat tgtctaatct ggaaaataag aaacggcata acctgtttgt
    27181 ccctgcaaga ggcgggattg gtgaagcgat aactgtacag gcaaattctg tcagtgcaag
    27241 aatggctgct aaagcaaacg gtaaccaccg cgcactttat gttccggaac aattaagtct
    27301 ggcaacttac aattctttac tcaatgagcc atctatccaa gaagtcttaa acttgattag
    27361 tgaagcgaat tgtgttattc atagtattgg gcgtgcatta cacatggcag cacgtcgtaa
    27421 gatgactgaa aaagaattag tcatgctgaa acaagcaaat gctgttgcgg aatcttttgg
    27481 ctactttttc aatgaaaaag gtgaagtcgt ttataaggtc cctagaatcg gcatagaatt
    27541 gcaagatttg gaaaaagtcc cgatcatcat ggcaatcgcg ggcggaaaat caaaagctaa
    27601 agcaattcga gcttatatga aaaatgcacc aaaacaaacg tggctcatca ctgatgaagc
    27661 tgctgcaaat gagattttaa aaggggtaac cctttaaaat aaatattttt ttgattttca
    27721 taaggaggaa atcttaaatg acagttaaag taggtattaa tggttttgga cgtattggac
    27781 gtctagcatt ccgtcgtatc caagatgtag atggaatcga agtagtagca atcaacgact
    27841 tgacagatgc taaaatgttg gcacacttgt taaaatacga cacaactcaa ggacgtttca
    27901 acggaacagt tgaagttcat gaaggttcat tcaacgtaaa cggaaaagaa gttaaagtat
    27961 tagctaaccg caacccagaa gaattaccat ggggcgaact aggcgtagat atcgttctag
    28021 aatgtactgg tttcttcact tctaaatcag cagctgaaaa acatttaaca gctggtgcta
    28081 aacgcgttgt tatctctgct cctggcggaa acgatgttcc aacaatcgtt tataacacaa
    28141 accacgaaac attaactggt aaagaaacag ttatctcagg tgcttcatgt actactaact
    28201 gtttagctcc tatggctaaa acattgaacg acaaatttgg tgttgttgaa ggattaatga
    28261 caactatcca cgcttacaca ggtgaccaaa tgactctaga cggacctcat cctaaaggtg
    28321 acttccgccg tgcacgcgct gctgctgcaa acatcgttcc taactcaaca ggtgctgcta
    28381 aagctatcgg tttagtaatt cccgaattga acggtaaatt agacggagct gctcaacgtg
    28441 ttcctgtacc aacaggttca ttaacagaat tagtaacagt tcttgaaaaa gaagtaactg
    28501 ttgacgaaat caatgcagca atgaaagaag cttcaaacga atcttacgga tacaacacag
    28561 acgaaatcgt ttcttcagat atcgttggta tgacttacgg ttcattattt gatgctacac
    28621 aaactaaagt aatgacagtt ggcgacaaac aattagttaa aactgttgct tggtacgaca
    28681 acgaaatgtc atacactgca caattagtac gtactttaga atacttcgct aacttataag
    28741 atagacaaac gtacaagtgt aaattataga catgtacatt taaaagcggg gaagcaatcg
    28801 cgcttcttcc gctttttttg ataataagtt tattcaggag gaaataaaat ggctaagaaa
    28861 acagtgaaag atatcgattt aaaagacaaa aaagttcttg tccgtgttga cttcaacgtt
    28921 cctttgaaag acggcgtgat cactgacgac actcgtatca aagcagcatt gccaacaatc
    28981 aactacgttc tagaacaagg cggaaaagca atccttttct ctcaccttgg acgtgtaaaa
    29041 acagaagaag ataaagaagg aaaatcttta gctccagttg caaaacgtct aggcgaatta
    29101 ttaggtaaag aagtaacatt cgtacctgaa actcgcggag aacaattaga agaagcaatc
    29161 cgcaacatga atgacggcga cgttgtcgta ttcgaaaata cacgatttga agatattgac
    29221 ggtaaaaaag aaagcaaaaa tgatacagaa ctaggtaaat actgggcttc attaggcgat
    29281 gtatttgtta acgatgcttt cggtacagct caccgtgcac atgcttctaa cgtaggtatc
    29341 gcttcaactg gtatcccaac agttgctgga ttcttgatgg aaaaagaaat caagttcatc
    29401 ggtgaagcag tagaagaacc aaaacgacca atgattgcaa tccttggtgg cgcaaaagtt
    29461 tctgataaaa tcggcgtaat cgaaaacttg attcctaaag cagataaaat cttgatcggt
    29521 ggcggaatga cttacacatt ctacgaagca aaaggaatca agatcggtaa ctctctagta
    29581 gaagctgata aagtggaact agcaaaagaa ttaatcgaaa aagcaggcga caaacttgtc
    29641 ttgccaatcg acaatgtctg tgcaccagaa ttttcaaatg atgttgaaac acaagtaatc
    29701 gaaggagaca ttcctgatgg tttgatggct ttagacatcg gcccagcatc agtaaaatta
    29761 tttgctgata cattacaagg tgcaaaaaca gttgtttgga acggacctat gggtgtattt
    29821 gaaatgagta actttgctaa aggtacaatc ggtgtttgtg aagcaatcgc taacttagaa
    29881 ggcgcaacaa caatcatcgg tggcggagac tcagccgcag cagcagaaca attaggattt
    29941 gctgacaaat tcacacacat ctcaacaggt ggcggcgcaa gcttggaatt gcttgaaggt
    30001 aaagaattac caggactagc agcaatcaac gataaataaa cgaccaagtt tgctcaattc
    30061 tgacgagtaa tcgaacaaaa ggtgaaggga gttttcatga ctccctagcc atttttcaat
    30121 aaattaagga gtgtgttcct caatgcgtaa acctattatc gcaggtaact ggaaaatgaa
    30181 caaaactgca tcagaagcaa aagaatttgc tgaagcagta aaaaacaaaa tcccttcaaa
    30241 tgacaaagtt gattcagtca tcggatctcc tgcattattc ttacaagaat taatgacatc
    30301 agctgaaggt acagaactaa aaatctcagc acaaaactgc tactgggaaa actcaggtgc
    30361 gttcactggt gaaacttcac cagcagctct tgctgatcta ggtgttcatt atgtgatcat
    30421 cggacattca gaacgtcgtg aatatttcca tgaaacagac gaagacatca ataaaaaagc
    30481 gaaagctatc tttgcaaatg gtatgacacc aatcctatgc tgcggcgaat ctttagaaac
    30541 ctatgaagca ggcaaaactg ctgaatggat cgaaggacaa attacagctg gtttagctga
    30601 tctttcagct gaacaggtgt caaacatggt tattgcttat gaaccaatct gggcaatcgg
    30661 tactggtaaa tcagctgacg cacaaatcgc tgacgaaatt tgtggagttg tacgtcaaac
    30721 agttgaaaaa ctttatggaa aagaagtatc agaagcagtt cgtatccaat acggcggttc
    30781 tgtaaaacca gaaaatattg ctgaatacat ggcaaaagaa aatgttgacg gtgctttagt
    30841 cggtggcgca agcttgcaag cagattcatt cttgtcattg ttagacgctg taaaataatt
    30901 gttgaaaatt tgcgaaaaat gcaagaaatg tttgcactaa tgagcaaaat taactaaaat
    30961 cgtatatgag ggaaatccct taggaaaaaa caaactcaaa ggagagacaa aacatgtcaa
    31021 ttattactga tgtttacgct cgcgagatct tagactcacg tggtaaccca acaatcgaag
    31081 tagaagtata cacagaaagc ggagcttttg gccgcggaat ggttccttca ggtgcttcaa
    31141 ctggtgaata cgaagcagtt gaattacgtg acggagacaa agctcgttat ggcggtaaag
    31201 gtgtaactaa agctgtcgac aacgtaaaca acatcattgc tgaagcaatc attggctacg
    31261 atgttcgtga ccaaatggct atcgacaaag ctatgatcgc tttagacgga actcctaaca
    31321 aaggtaaatt aggtgcgaac gctattttag gtgtttctat cgctgttgca cgtgcagctg
    31381 ctgactacct agaagtacct ttataccact acctaggcgg attcaacaca aaagtattgc
    31441 caacaccaat gatgaacatc atcaacggcg gatcacatgc tgataactca atcgacttcc
    31501 aagaattcat gatcatgcct gttggcgctc caacattcaa agaagctcta cgtatgggtg
    31561 ctgaagtatt ccacgcatta gcttcaatct tgaaagctcg cggattagca acttctgttg
    31621 gtgacgaagg tggatttgct ccaaaccttg gttcaaacga agaaggtttt gaagtaatca
    31681 tcgaagcaat cgaaaaagct ggctatgtac ctggtaaaga cgttgttctt gctatggatg
    31741 ctgcttcttc agaattctac gacaaagaaa aaggtgttta cgtactagct gactcaggcg
    31801 aaggcgaaaa aacaactgac gaaatgatca aattctacga agaattagtt tctaaatatc
    31861 caatcatctc aatcgaagat ggattagacg aaaacgactg ggacggattc aagaaattaa
    31921 ctgacgtatt aggcgacaaa gttcaattag ttggtgacga cttgttcgta acaaacactc
    31981 aaaaattatc tgaaggtatc gaaaaaggaa ttgctaactc aatccttatc aaagtgaacc
    32041 aaatcggtac attaacagaa actttcgaag ctatcgaaat ggctaaagaa gctggctaca
    32101 cagcagttgt atctcaccgt tctggtgaaa cagaagattc aacaatctct gatatcgctg
    32161 ttgcaacaaa cgctggccaa atcaaaactg gttctctatc acgtactgac cgtattgcta
    32221 aatacaacca attactacgt atcgaagacc aacttggtga agttgcagaa tacaaaggtt
    32281 tgaaatcttt ctacaactta aaaaacaaat aattcttttc taatgaagat acatgatcga
    32341 ctattgaaag atagtcgagt atgatcactt agttaagtaa tcgaactctc tacttgctga
    32401 aatatcagca ggtagagagt ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa
    32461 tggtacaaga aaagatcatc cttgaaaaag gaaagctaac aataactagc atcatataag
    32521 agcttttaag gatagaacag tagaatagga atctatgggc aagtcctctc gtataaataa
    32581 aataggaggg aagtagcagc atatttttca taaagtggct ctctttcttc atttttactt
    32641 tggttgctcc tatttgaaaa ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg
    32701 agaggattag atgaaaaaag aagaaaaaat cgcgatttta caagagatta tccgaattaa
    32761 atcagtgaat ggaaacgaag gtgaagtagc agcctatttg aataagcttt tggcaaagca
    32821 tgacataaca ggagagattg tttcttacag agatggtaga gataatttga ttgctcgtta
    32881 ccaaaagggc caatcaggaa aagtgttagg cctatctgga catatggatg tcgtggcggc
    32941 tggtgatgaa tcgtcttgga cctatgcacc ctttgcagca gaaatccatg ggaaccgttt
    33001 gtacggacgt ggtgctacag atatgaagag tggcttggca gcaatggtga ttgccatgat
    33061 cgaactgaaa gagtcaggaa aaccatttaa cggaacagtc aaattattag ccactgtcgg
    33121 agaagaagtg ggtgaactag gtggggaaca gctcacgaaa gcaggctatg tagatgactt
    33181 agatgccttg atcatcggag agccaaccaa ttacagtttg atgtatacgc acatgggatc
    33241 aatcaactat acagtcacct cgcacggaaa agaagcgcat agctctatgc cagaccaagg
    33301 ttataatgca atcaatcacc taaatgaatt cattacgaaa gcaaatgctg aaatgaatca
    33361 cctcgctgaa acaatcgaga atcctgtcct aggaaaaaca atccataatg ttacattgat
    33421 cagtggcggg aatcaagtca acagcatccc aagccatgcg cagctccaag ggaacattcg
    33481 atcgattcca gaatacccaa atgataaaat cattgcattg ctgcaatcaa tcgtcaatga
    33541 attgaatcaa gagacagact atcatttgga attgatgatc gattacaaca aaatacctgt
    33601 caaagctgat ccagattctc ctttgattca ttgcattcag caacaattca gccagccatt
    33661 acctttagtt ggagcggcag caacaacgga tgcagccgaa tttacgaaag ctaaccattc
    33721 cttcgacttt gtcgtctttg ggcctggtgt cgttactttg cctcatcaag tagatgagta
    33781 tgttgaaatc gataattatt tggatatgat cgaaaaatat caaggaatta ttttatccta
    33841 cttagcgtaa gattttttta ataaacgccg tttgtataat taagcgagac aaataaaaaa
    33901 gccatttatg ttacactc
//
LOCUS       DACDKJ010000024        32178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDKJ010000024 DACDKJ010000000
VERSION     DACDKJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.024"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(24..203)
                     /locus_tag="IV328_001308"
                     /pseudo
     CDS             complement(24..203)
                     /locus_tag="IV328_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(407..766)
                     /locus_tag="IV328_001309"
     CDS             complement(407..766)
                     /locus_tag="IV328_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8937458.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="IV328_001310"
     CDS             complement(796..1137)
                     /locus_tag="IV328_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937459.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="IV328_001311"
     CDS             complement(1134..1808)
                     /locus_tag="IV328_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8937460.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IV328_001312"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IV328_001312"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8937461.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="IV328_001313"
     CDS             complement(4322..5512)
                     /locus_tag="IV328_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8937462.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="IV328_001314"
     CDS             complement(5533..6060)
                     /locus_tag="IV328_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8937463.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="IV328_001315"
     CDS             complement(6107..8896)
                     /locus_tag="IV328_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8937464.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="IV328_001316"
     CDS             complement(9043..9243)
                     /locus_tag="IV328_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8937465.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="IV328_001317"
     CDS             complement(9696..10016)
                     /locus_tag="IV328_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937466.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="IV328_001318"
     CDS             complement(10519..11637)
                     /locus_tag="IV328_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937467.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="IV328_001319"
     CDS             12108..13415
                     /locus_tag="IV328_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8937468.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="IV328_001320"
     CDS             13536..14735
                     /locus_tag="IV328_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8937469.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="IV328_001321"
     CDS             complement(14762..16030)
                     /locus_tag="IV328_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8937470.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="IV328_001322"
     CDS             complement(16200..16841)
                     /locus_tag="IV328_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8937471.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="IV328_001323"
     CDS             complement(17250..17753)
                     /locus_tag="IV328_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8937472.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="IV328_001324"
     CDS             complement(17876..18640)
                     /locus_tag="IV328_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8937473.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="IV328_001325"
     CDS             18748..19023
                     /locus_tag="IV328_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8937474.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="IV328_001326"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="IV328_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8937475.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="IV328_001327"
     CDS             complement(20579..20980)
                     /locus_tag="IV328_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8937476.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="IV328_001328"
     CDS             complement(20994..21803)
                     /locus_tag="IV328_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8937477.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="IV328_001329"
     CDS             complement(21790..22680)
                     /locus_tag="IV328_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8937478.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="IV328_001330"
     CDS             complement(22693..23172)
                     /locus_tag="IV328_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8937479.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(23348..24289)
                     /locus_tag="IV328_001331"
     CDS             complement(23348..24289)
                     /locus_tag="IV328_001331"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP8937480.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IV328_001332"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IV328_001332"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8937481.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="IV328_001333"
     CDS             complement(25274..26470)
                     /locus_tag="IV328_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8937482.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="IV328_001334"
     CDS             complement(26488..27216)
                     /locus_tag="IV328_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8937483.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="IV328_001335"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="IV328_001335"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8937484.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="IV328_001336"
     CDS             complement(28628..29086)
                     /locus_tag="IV328_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8937485.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="IV328_001337"
     CDS             complement(29083..30546)
                     /locus_tag="IV328_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8937486.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="IV328_001338"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="IV328_001338"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8937487.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="IV328_001339"
     CDS             31602..>32178
                     /locus_tag="IV328_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8937488.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6920 c         5511 g        10362 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcaacaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttgtcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DACDKJ010000025        31882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDKJ010000025 DACDKJ010000000
VERSION     DACDKJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.025"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(56..1351)
                     /gene="purB"
                     /locus_tag="IV328_001340"
     CDS             complement(56..1351)
                     /gene="purB"
                     /locus_tag="IV328_001340"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8937489.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1364..2497)
                     /gene="purK"
                     /locus_tag="IV328_001341"
     CDS             complement(1364..2497)
                     /gene="purK"
                     /locus_tag="IV328_001341"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8937490.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2481..2969)
                     /gene="purE"
                     /locus_tag="IV328_001342"
     CDS             complement(2481..2969)
                     /gene="purE"
                     /locus_tag="IV328_001342"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8937491.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3262..4569)
                     /locus_tag="IV328_001343"
     CDS             complement(3262..4569)
                     /locus_tag="IV328_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8937492.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4572..5153)
                     /locus_tag="IV328_001344"
     CDS             complement(4572..5153)
                     /locus_tag="IV328_001344"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8937493.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5269..5366)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5725..7017)
                     /locus_tag="IV328_001345"
     CDS             complement(5725..7017)
                     /locus_tag="IV328_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8937494.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7014..7637)
                     /locus_tag="IV328_001346"
     CDS             complement(7014..7637)
                     /locus_tag="IV328_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937495.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7640..8476)
                     /locus_tag="IV328_001347"
     CDS             complement(7640..8476)
                     /locus_tag="IV328_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8937496.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8493..9380)
                     /locus_tag="IV328_001348"
     CDS             complement(8493..9380)
                     /locus_tag="IV328_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8937497.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9388..10623)
                     /locus_tag="IV328_001349"
                     /pseudo
     CDS             complement(9388..10623)
                     /locus_tag="IV328_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(11306..11989)
                     /locus_tag="IV328_001350"
     CDS             complement(11306..11989)
                     /locus_tag="IV328_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8937498.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12007..12624)
                     /locus_tag="IV328_001351"
     CDS             complement(12007..12624)
                     /locus_tag="IV328_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8937499.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12634..13458)
                     /locus_tag="IV328_001352"
     CDS             complement(12634..13458)
                     /locus_tag="IV328_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8937500.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13451..14011)
                     /locus_tag="IV328_001353"
     CDS             complement(13451..14011)
                     /locus_tag="IV328_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937501.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14185..15141
                     /locus_tag="IV328_001354"
     CDS             14185..15141
                     /locus_tag="IV328_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8937502.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15446..16402)
                     /locus_tag="IV328_001355"
     CDS             complement(15446..16402)
                     /locus_tag="IV328_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8937503.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16506..17108
                     /locus_tag="IV328_001356"
     CDS             16506..17108
                     /locus_tag="IV328_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8937504.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17196..18695)
                     /locus_tag="IV328_001357"
     CDS             complement(17196..18695)
                     /locus_tag="IV328_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8937505.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18711..19235)
                     /locus_tag="IV328_001358"
     CDS             complement(18711..19235)
                     /locus_tag="IV328_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937506.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19533..20282
                     /locus_tag="IV328_001359"
     CDS             19533..20282
                     /locus_tag="IV328_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8937507.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20273..21058
                     /locus_tag="IV328_001360"
     CDS             20273..21058
                     /locus_tag="IV328_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8937508.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21027..21743
                     /locus_tag="IV328_001361"
     CDS             21027..21743
                     /locus_tag="IV328_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8937509.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(21845..22864)
                     /locus_tag="IV328_001362"
     CDS             complement(21845..22864)
                     /locus_tag="IV328_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8937510.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(22883..23941)
                     /locus_tag="IV328_001363"
     CDS             complement(22883..23941)
                     /locus_tag="IV328_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8937511.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24161..25309)
                     /gene="nagA"
                     /locus_tag="IV328_001364"
     CDS             complement(24161..25309)
                     /gene="nagA"
                     /locus_tag="IV328_001364"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8937512.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25385..26221)
                     /locus_tag="IV328_001365"
     CDS             complement(25385..26221)
                     /locus_tag="IV328_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8937513.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26405..27622)
                     /locus_tag="IV328_001366"
     CDS             complement(26405..27622)
                     /locus_tag="IV328_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8937514.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28005..28088
                     /locus_tag="IV328_001367"
     tRNA            28005..28088
                     /locus_tag="IV328_001367"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28039..28041,aa:Leu,seq:gag)
     gene            complement(28328..29698)
                     /locus_tag="IV328_001368"
     CDS             complement(28328..29698)
                     /locus_tag="IV328_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937515.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(30412..30702)
                     /locus_tag="IV328_001369"
     CDS             complement(30412..30702)
                     /locus_tag="IV328_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937516.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(30910..31602)
                     /locus_tag="IV328_001370"
     CDS             complement(30910..31602)
                     /locus_tag="IV328_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8937517.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         9527 a         6714 c         5387 g        10254 t
ORIGIN      
        1 ttttttggtt atttttgcta ttctatttta attgaatttt gcaactcact cttctttatt
       61 ccaaacctac tcgtttgaat atcacgtcaa cgtttttcaa atgatgatga taatcaaagg
      121 catcgtctat ttcttcttta gataataccg atgtgatggt tggatcttct tctaacagcg
      181 ggcggaactg cacttggttg tcccaagcgt acgcagtttt tggctgaaca agatcataag
      241 cttcttctct tgtcattccc ttatcgatca atttcagcat aacgcgctgg ctataaatca
      301 aaccaaatgt tgcagacata ttccgcttca tgttttcagg aaagaccgtt agatttttaa
      361 cgatattgcc aaaacgattt aacatgtaat tcaataaaat cgtggtatca ggcaagatga
      421 ttcgttctgc tgaagaatga gaaatatctc tttcatgcca taatgtgaca ttttcatagg
      481 cagtgaccat gtgtccgcgc accactctgg ctagaccagc catattttct gaaccgatcg
      541 gattcctttt gtgcggcata gcagaagaac ctttttgacc tttcgcaaaa aattcttcta
      601 cttcacgtgt ttcggatttt tgcaatccgc gtatctctgt tgcaaaacgt tcaatacttg
      661 tagcaataag cgccatcgct gagaaatatt cagcatgtag atcccgaggg agaacttgag
      721 tagagatttc ttgtggacgg atccccaatt gtttacatac atactcttca ataaatggcg
      781 ggatatttgc gaacgttccc actgctcctg agatttttcc tgcttctact ccttttgccg
      841 catgttcaaa tcgttcgatg ttccgtttca tttctgaata ccatgtggca agtttcaatc
      901 caaaagtagt tggttcagca tggacaccgt gtgtccgacc catcatcact gtatatttgt
      961 gttcttttgc tttttcgcca atgatttccg taaaacgttt caagtcttcc cgtaaaatat
     1021 catttgcttg cttgatgaga tagccataag cagtatctac gacatccgta cttgttagac
     1081 cataatggac ccattttctt tcttctccca atgtttcaga aaccgctcgt gtaaacgcaa
     1141 cgacatcatg gcgtgttaat ttttcgattt ccaagatccg atcgatatca aatgatgcat
     1201 gctcacgaat ttttttcaca tcttcttgcg ggatttcacc aagttctgcc caagcttcat
     1261 ctgctaaaat ctcgacttcg agccacgcac ggtatttgtt ctcttccgtc cagatattcc
     1321 ccatttcagg gcgtgtataa cgttctaaca tgttttttct cctctaatcc caaatattcg
     1381 tttgtttgat ttcttccagc gtttgctgga tgtcttctgt caagatagtg atatgtccca
     1441 tcttgcgacc tttttttgct tgtttttttc catagaaatg gaattgccag tctggcttat
     1501 tctcaatcaa gcgatagctt tccagcagtt gttctcccaa aacattgacc attactgcat
     1561 tgcttatcaa acgtactttt tctcctaatg gccaaccaca aatacctcga atatgggcat
     1621 cgaactgact catagaacaa gcttcaatag aataatgtcc agaattgtga ggacgaggag
     1681 ctaattcatt cacatatagc ccgcctgttt ttgttaaaaa catttctacc cctaaaacac
     1741 ctgaaaggct cactgcttct gcaatcacac gggcaatacg ctcagcctcc tcgatcacat
     1801 cctgatcaat cgcggctgga gcaatcgttt catgaagaat attgttgtgg tggatgtttt
     1861 ccacgaccgg aaaagtcgtg aaatctcctt gaccatttcc tgccaccatg atcgaaatct
     1921 ctttttcaaa cgggatccat gcttctaaaa cacaggttcc ttctcttaaa aggtccatgg
     1981 caggagctag atccgcccga cttttcaaaa catactgccc cttgccatca tagccgcctc
     2041 gagtggtttt caaaacacaa ggatagccga tcccgtcaat cgcatcttgg atatccgtcg
     2101 gactgacgat cgttgcataa ggagcaatca cgatgttatt cgtttccaaa aaagattttt
     2161 ctaacaggcg gtcttgcgta atcgcaagca agtctgtccc ttgaggaata aatgacatgg
     2221 gtaaaatcgc atttaacgcc tctacactga cgttttcgaa ttcataggta atgacatctg
     2281 ttcttctggc aagctctttc agcgcaaagg tgtcatcata gacactttca atgtgccaat
     2341 ctgcaacttg tgcggctgga caatcgctca caggatccag cacacctact ttaaagccca
     2401 tttcttttgc actaatcgcc atcatacgac ctaattgacc gccaccgacg atcccgatcg
     2461 ttgaaccagg caacaaaggt ttaaccaagt tgatcactac tttctataac agttttagcc
     2521 aacattgaac gtctttcagc caatttagcc tccaattcta aatcatacat ggaaagcatc
     2581 tgtatcgcaa gtagtccagc attaattgct cccgcttttc cgattgctgt agtagctaca
     2641 ggtactcctc caggcatttg gacaatcgaa agaagcgaat ccaaaccatt taatgttcgt
     2701 gattggaccg gtacaccgat aaccggcaaa gttgtttttg atgcgaccat acccggcaga
     2761 tgcgcggctc cccctgctcc tgcaataatg attttgattc ctttttttcg agcgttttgt
     2821 gcatattcaa acatcaaatc tggggttcga tgagcagaaa cgaccttttt ttcataagac
     2881 acaccgaatt catccaatat ttgacatgct tctttcattg tttcccaatc tgaagtactc
     2941 cccataatca cagagatttt tgctgtcatt gtttttcctc ccagtttttc tacattcata
     3001 ttttaacaat cacttttttt gatgtcaaag aaatttcgaa tgttaacaaa gaaaaacatt
     3061 ttattgttcg tgtttattaa agatgaaaaa aaggaatgtg acaaaagtta ggtcacattc
     3121 ctttttatgg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aacaaacggc
     3181 aaaatacgag gaactatttt tccgtttgtt tctctaacaa tcaggtcaaa acgctgattt
     3241 cacaaccttt cttttgctct attacttctt gaaggcttct tccaatattt cttcatcttt
     3301 tttcaatcca ttaaagatca aattcaatat gatagccgta atgctactca ttacgatgcc
     3361 atttccagtg aacatttgca atgtttgcgg catttgactg aataatgtcg gcgtaacatt
     3421 gaaacccagc ccaaatccaa cagaaacagc aacaatcaat agatttttat cattgctgaa
     3481 atcaacttcc aacagcatac ggattccttg gactgccacc atcccgaaca tgactaacat
     3541 tcctccacca agtaccggct cagggatgat ttgtgctaac gcaccgattt tagggaataa
     3601 tcctaaaaca atcaagaaga atgcagagaa atagatgggt tttcttgttt tgattcccga
     3661 aagttgaacc aatccgacat tttgagaaaa gccagtataa gggaatgtat tgaaaatacc
     3721 gcctaagata acggcaagtc cttctgcacg atatcccttt ttcaattctt cttctccaac
     3781 atttttccca gtaatgtcac caagtgcaaa atatacacct gttgattcta ccatgctgac
     3841 catggaaatg atgatcatta gtaaaatcga ccatacgtcg aaagttggtt taccaaaata
     3901 aaagggttgt ggaacatgga acaacggcgc atctccaatc gcgctcagat caatcattcc
     3961 catgaaagca gctaagatgc ttcctccgac taaaccaatc aataccgcga ttgaacgaat
     4021 gaaccctttt gccagcattt gcacgcctac aattaggaga atcgtcacaa atgcaagtag
     4081 cagatttgtt ttgtcaccaa aatcagctgc tgttgcatta cctccaccca ttttctcgat
     4141 tgctacagga atcaaagtca aaccaataac ggtgataacc gttcctgtta ctataggagg
     4201 gaatagtttt ttaattttag aaaagacacc agaaactaac acaacaaaga tcccagaagc
     4261 gataatagat ccgtagatag ctcctactcc ttcattggtt ccgatcaaga tcagtggagc
     4321 tactgcttgg atcgcacatc ctaaaacgac aggcaagcca attccgaaaa atcggttaac
     4381 tgtcaattgc aataatgtcg ctaatccaca cataaagata tcaattgaaa tcaaataagt
     4441 catctgttct tgattaaagc ctagtcctgt accgatcaaa agaggcaccg ctactgctcc
     4501 tgcatacatc gctagcagat gctgcagccc taaaaccgca gcttttccat tttgtgtttc
     4561 tttttccacg attatgcgtc ctcctcaaga aactcaactt gtccattcgt caaggaagca
     4621 atacgcgcaa gagaaacgac tctcaatccc atttcttcaa gtaactgacg tccatcttgg
     4681 aaagactttt cgatgacgat cccgattcct tctacttgcg ctccagcttg ctggcataat
     4741 tcgatcaatc cttttgccgc ttgtccgttt gccaagaaat cgtcgataat caatactttg
     4801 tcttctgaag ataaaaattt acgagaaata gaaatcgtac ttgtcacttg tttcgtaaaa
     4861 gaataaacag aagctgtcaa taattcttca tccatcgtca aactttttgc ttttctagcg
     4921 aagatcattg gaacatctaa ttctcttgca gcaaataaag ctggtgcgat cccagaagct
     4981 tcaatggtca caactttcgt gattccttgt tctttaaata cttctgcaaa ccgttttccc
     5041 atttgttcca tcaatacagg gtctacttgg tgagtgacga agctgtcgac ttttaatacg
     5101 ccctcgccta atatacgtcc gtctttttga atacgttcaa ctaattcttt cactttattt
     5161 gctcctttat catttattaa taccgaaata accgcttttg tgcaaaaacc tactatgttt
     5221 ctgttaaaga aaaacaccct ccactttatc ataagaaaag aagggtgaaa tagccaatct
     5281 tttacttata gtccagcatt tacggtactg gctagaaact gtcatccata ttatgacgat
     5341 atataagtag gtatgtaaaa ctaataatag tgtaaattca ttttcttgca actgttttgc
     5401 acgtgaaggc agtttgccat atttgaataa aaaatgttgt ttcaaatatt gaaaacagtc
     5461 taacactact ttctgaaact gacaacgtgg tttacttatt tttttgtaaa atctaatgat
     5521 ttttaatgca ttattccttt ttgttcgggt ttaaatagtt atttttattt tctcctatct
     5581 aaaaaagcag cccacataaa aagaaggcat gacaaaaagc atgtcatagc ccctttttcc
     5641 gaataaatag tgttcaaaag acagcctatc ggcaataagg caagatttta taaactacgg
     5701 gaagtaattt ccgtaaatct attcttattc ttttaagctg acaagctcgt caatcactac
     5761 tgcttttcct gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg acaaaatacc
     5821 atctacgtga gttgctgccc gattccacgg atcttcttct ttttcttcac caaatagatc
     5881 atctaataaa cgcggatctc ctccgccatg tccgcccgct tcttgttcga cgattacctc
     5941 atatggctca ccgaacatcg gatagacatg gatggactga agaacggctg ctccttcatc
     6001 ctttttatct ccacctgcat tgatgtaaga cttttcgatc actttcattt cgatccgtcc
     6061 tttgctgcca ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt ttagcgaata
     6121 cgtcaaaatc gcaccagttt catatttgac cgttacgcct agtgtatctt caatagagat
     6181 atcatcggaa aatacactgc ggtcacgaat atatccactg tctttttctg cttctaaata
     6241 catttctcgc aattcctcat tggctgacag gtctatagca aatggatcag ctttagctag
     6301 ttcgcttcca tagcatcgac tgtaaaatat ttctactccg cgtttctccg cattatgcat
     6361 cccataaaag tttaatccac cgaatgcaaa taccttttgg ggacggctgt tcaaccaaaa
     6421 attcactaga tcaaaatgat gggtagattt atgaacaaga agcccgccgc tattgtgctt
     6481 atttcgatgc catcttctga aataatctgc cccgtgttgc gtatttaaca gccattcgaa
     6541 gtggatagaa aacacatcac caatcacatc actttctatc agttccttta tcttcgtgtg
     6601 atacggtgca taacgataat taaaagtgac tcgcaggctt cttcccgtct gtttttggac
     6661 cgttattatt tcttgagctt tttcctcatc gatagtcaga ggtttttcgc aaataacatc
     6721 atatcctttt ttcattgcac gaatgatata atcatgatgc gttcgatcta ctgacgtaat
     6781 aatgacttca gccggccgta cgtcatccag tagttgctca aattgtgatg gcatacatgt
     6841 ctttactttt ttctgtccat acttgttcac aagtatccga tttgcataat ccatccttgt
     6901 ttgagataca tcacaaaaac cgactagttc acaagtttga tgatacgtag aggcaattgc
     6961 ctcataaaaa aaacgcgctc tcccaccaag tccgacttgt acatattttt tcttcattta
     7021 aagtcattct ccttttcttt taagcaaaat actgtgacaa atggcagccc tggatacagt
     7081 aatgcagtga gtaaaaaatc aggctgtata caccagaaca gtccatacaa aaggcataaa
     7141 taccacagaa accccttgac gttttttgac aacagccaga tattcctttt gatcaaataa
     7201 acaagactat ctgctttatc ttctttgcag atatcaatta gcgtactaaa aaaactatgg
     7261 gatacaaaaa gcaacagaca caatagaaaa ggaatcagat accaagctcc aatctttccc
     7321 actaataaat accaatcaac tgcaaaaata atgacaaaca gctgaaacaa cgtaatgatg
     7381 aaacacatcc gtttgatttt ccctttcctc caacatacct ttttttctgc caagtaatgg
     7441 acagatagat aataagaagg atacatcatc aaaaaataaa tcagatagac aacacgagca
     7501 gacagcacta atcctaaagt gaacaaacat cctagaacta aagcgaaaag agcattactg
     7561 ataagactgg ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag aaacatctct
     7621 tctattggct ttttcatatt cagcccttca gaccgcttgt actgatcccc tggacaagat
     7681 atttattgaa gaataagaaa atcagaaaaa ccggtagcag cgacaacgta gacatcgcaa
     7741 acatactcgc gtaagtcgta ctagatccag cgtctgcaaa taatttgatg gcaatcgata
     7801 ccgtgtaaga tctcggcttg tttaaataaa tcaatggtcc catgaagtcg tcccatttcc
     7861 aataaaactg gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga aaaataattt
     7921 tggtaaagac actatacttg ctgcatccgt caataatcgc tgattcatct aactctttgg
     7981 ggatgctgac catgaattgc atgatctggt aaatgaaaaa tgcttgtcca aaaaaatgtg
     8041 gtaaaatcag cggcacatac gtcccaacca aattcagccg attgtaaata ataaactgcg
     8101 gaatcaaaat cacttgtgca ggaatcatca tcgttactaa catgacgacg aaccagaatc
     8161 tttttccttt gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag ctcagaactg
     8221 tccctatcgt cgaaactgca gagatgacaa acgaattcct aaagaaagta aaaaatgaaa
     8281 ttccagcaaa ccccgcccat ccttttgcgt aattttccca ttgaaaagtc tctggaatca
     8341 aattcagcga accactcata atctcttgat ccgttttaaa agaacctgcg atcatccata
     8401 agaccggata aatcatgact agacctaata gcaaaataaa accgtattga aaccatttcg
     8461 ttactgtatt attcatttct tcctcctctc attcacacaa cgacccattt ttttgatgat
     8521 ttgaaaatca gaaaggtgac agctcccata atcaacagca tgatccacga aatagcacta
     8581 gcatacccca tagaattaaa agaaaaagct tggttataca cataaagagc ataaaagttc
     8641 gtggcatcat tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt cataaacgcc
     8701 gcgattgtct gcataaccaa gttatacaaa atgatcggcg aaagaagagg taacgtgatc
     8761 ttgtaaaacg tctgccattt gttcgctcca tcgatctctg ccgcttcata atacgagcct
     8821 gggatctctt tgatacctgc agcaaagatg atcattgccg aaccgaattg ccaagcagac
     8881 atcaaaacaa gagggatcat gaccagtttt tgatcgctaa accaggaaag cgcaggtgcg
     8941 cctaatcgtt ccaggaaaat attcactaac ccgtttttag aaaacaactc ccgccaaact
     9001 agagcgatag aaatactgcc accgatcaaa ctaggcacat aatacaaaga acgaaagaaa
     9061 gtcgttcctt tgatattcct cgttaaaaga taagcaatca atagaccaaa gatcagtttc
     9121 acaggtacag atgcaaatac atagagaaaa gtgactcgga tggagttcaa aaaacgggca
     9181 tcactcagca tctttatata attatccaag ccgatccatg agactggatt tcccaaacga
     9241 taatcggtga acgaataata aagtgacaag atcatcggta tcactgttat caatagaaat
     9301 cctaggatga atggagaagc aaatacgtaa ccgaccacat tgtcttgatc caagaaccgt
     9361 tttagttttt cttttttcat gccttccttc tgctgttact tcttcataaa tgactttttc
     9421 aactaaaaga ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac
     9481 ttcgccgttg tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc
     9541 atcatctgca cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc
     9601 ttttaaaatt tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc
     9661 ttctttatgg gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc
     9721 agattctcca tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg
     9781 agcccctgac aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct
     9841 atccctaacc gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg
     9901 atgggcccct ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg
     9961 attttcaaat cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc
    10021 aacttgtggt atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata
    10081 gattcctaat tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt
    10141 tgaagctggt tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa
    10201 cgaattcacc ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata
    10261 ggattcatct gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc
    10321 aatctgattg atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc
    10381 tgatgctgct agtgtaccta attttgtgta atatccttca taatcataaa attcataatc
    10441 gatcgtcaca tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg
    10501 tcggacttgc gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc
    10561 acaagccgtc aagaagaata acataacact taaagctaag atgattctca ttcttttctt
    10621 cattctctgc acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac
    10681 atatttatta aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg
    10741 cctaggatga acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat
    10801 cgctaaaaac acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat
    10861 cagacaactg cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct
    10921 tccctaaaaa acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt
    10981 cgatgcacaa gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc
    11041 ccgacaattt agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca
    11101 ttctatagaa agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca
    11161 aaaggtttta actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt
    11221 tttgaattct gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc
    11281 ttcctcgtct tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt
    11341 tttccatttt acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg
    11401 ctcctgcttg aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag
    11461 gaagttcagg atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac
    11521 tgatattgat aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg
    11581 ttaccgtact gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag
    11641 gcgtaggacc gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt
    11701 aggaagaacg agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag
    11761 ctgcctcaat aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta
    11821 ctgcattcgc atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga
    11881 tcaattttcc aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga
    11941 tatcttttct caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct
    12001 cctttgctat ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt
    12061 ttgcgcataa ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg
    12121 atagttggtg gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac
    12181 gaacagttgt gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc
    12241 ttggatagtt atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg
    12301 atccccataa gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc
    12361 ccaatttgat tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc
    12421 ttgtagttct tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc
    12481 attttcttgc tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc
    12541 taagagtatc actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg
    12601 ctttttccac ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata
    12661 ttcttctagc gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg
    12721 taagtgccaa ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac
    12781 gataaatcca tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta
    12841 acttgcatcc aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac
    12901 tgccagacct gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt
    12961 tgtcgcctcc tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa
    13021 aacttgttgt tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc
    13081 tgcttcagca gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta
    13141 tccgttactt aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc
    13201 tggaccaacc aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt
    13261 acgatctttt gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt
    13321 actagcagtt gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga
    13381 atcatcatcg ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa
    13441 tcctaaaaat ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct
    13501 catgaacgta agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc
    13561 cttcgatctg tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc
    13621 attcatcata ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt
    13681 tcaaagggat cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg
    13741 ttttgatttg cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt
    13801 gacgcaaaat cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct
    13861 gcatcgcttc agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac
    13921 tgatcagcaa gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac
    13981 tcataataag atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact
    14041 taagttttga tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag
    14101 tcctctcctc tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat
    14161 ccaactatga aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt
    14221 accatcgcac atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt
    14281 gcttccagaa cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct
    14341 tacggaagct atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta
    14401 ccaaatagac agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta
    14461 tgtgaaaaag ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa
    14521 gaaaagaatt tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag
    14581 ttacgctcaa taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc
    14641 ggcagttaca aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga
    14701 gctctgttgg atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa
    14761 cctgaagtag ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc
    14821 actatcttgc gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa
    14881 atgccaaaag tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg
    14941 cgtgctgatc gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg
    15001 aatacatctg aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac
    15061 ttgcccaatc gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa
    15121 gttctatgga aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa
    15181 taagaacagc caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc
    15241 agaggaaagc ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga
    15301 gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga
    15361 ccgaggagtt gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc
    15421 tgaatattag ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct
    15481 tgatttcaat aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga
    15541 attcagggcg actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac
    15601 gtgttaaaat ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata
    15661 aaccatagat atgttttcca taataagctg gttgctggat tctctctaga ttttccagca
    15721 tgtctttaat cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg
    15781 gctgtctgag ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga
    15841 atgtcagtaa atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt
    15901 ctgccatttg tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca
    15961 gctgcttcgt acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg
    16021 ccattaatcc cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat
    16081 gagtgaccaa aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg
    16141 ggatgaacag gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac
    16201 tgaacagtgc tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca
    16261 atgtagagcg cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat
    16321 acaacagcga taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga
    16381 ctgttcgcat tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa
    16441 tgaaaaacta gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa
    16501 tctctatgca atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg
    16561 tttttttatg tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat
    16621 tatctgctgt tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta
    16681 ggatcatcgg caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct
    16741 attttcacaa tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga
    16801 tcgtcgtgtc agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt
    16861 tgctgattgc gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac
    16921 tagatacttt tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg
    16981 aaacagaaaa gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat
    17041 tgctaagaac aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg
    17101 aggactaatt ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt
    17161 cctccatttt tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt
    17221 tctgcctctt ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa
    17281 ttctctacga tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa
    17341 gaaagcaaat ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg
    17401 cttcctgtat cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc
    17461 atcaattgcg gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg
    17521 aaggcattgg cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct
    17581 tcggcttctg cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc
    17641 acacggaatc gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct
    17701 tgttctttag cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt
    17761 tcacgacgtg tgatttcttt ttcttccagt tcgatttgtt tttggcgttc tacgatttta
    17821 atctgcattt cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct
    17881 tggtctgctt ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct
    17941 ttttctttta atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct
    18001 ttttctgctt gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct
    18061 gcatctcttt tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg
    18121 tctctgacct ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca
    18181 gaagcgactt cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca
    18241 gtcatagaac caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc
    18301 agttcttctc ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat
    18361 gaaccgattt tgatgatcga agtaccatca cacatcaccg gtacgccttg ttcggtatac
    18421 acttcagggg tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga
    18481 aaaactggca agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca
    18541 tcaatgtgca catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt
    18601 tttgctgtct ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg
    18661 attgcgatcg cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt
    18721 atcatgcttt cataaggcat gacataacag attctttcct tgatctcaat gatcaatact
    18781 ttcgttcccc gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg
    18841 gaaccggtaa cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc
    18901 acaacagcta cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt
    18961 ttcatcggca tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca
    19021 ctagcaataa ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat
    19081 aagcttgtga aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg
    19141 aatacatctc caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac
    19201 caataaatcg tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg
    19261 tctatcatac caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt
    19321 ttataggaag gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat
    19381 tttcacaata aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa
    19441 agctccagaa cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt
    19501 ctgccatatt gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa
    19561 tctctagcat tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg
    19621 gtgacccgac acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag
    19681 attttatcgt tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca
    19741 tcggtcttcc tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt
    19801 tctctgaaaa agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg
    19861 atcaagagat tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag
    19921 tcaaagatgg acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca
    19981 taggcgccgt ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag
    20041 aaatagcaga acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct
    20101 tagccatctc ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct
    20161 accaaacaga gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga
    20221 cgttttgttt aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt
    20281 aaatgggata gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg
    20341 agcagtcgca actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg
    20401 gacgaaacaa tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca
    20461 tgtcatttct ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca
    20521 gctaaatcaa aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc
    20581 aattgccggt tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct
    20641 gttagaaagc gattctttta tccctaaaat ggagatggat gactccatcc attcactttt
    20701 gcaagtgtta gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag
    20761 aaacggacag gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac
    20821 cgtctgcgat ggcggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg
    20881 tttttcgacg aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa
    20941 acgccacggt ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct
    21001 agaattacca aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc
    21061 caatggttgc agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg
    21121 aaagtttcag aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc
    21181 tgctgcttga tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac
    21241 agcaaggaaa tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa
    21301 agaatgtgat ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt
    21361 ttcagctggc gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag
    21421 ccgatcagaa attgatcgat caagtattca accacaccac tcccattcac gaaataccat
    21481 cattcatcga tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta
    21541 ttctaaaaga agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac
    21601 gtatatccgt caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa
    21661 tcaaatattt ggaaatcggc cagccgctaa cctgttatga attgacaaag aatgggcgga
    21721 aaaagctcac agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata
    21781 cagaagtaac aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt
    21841 tttcttatct taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt
    21901 ctattatttc acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg
    21961 tatttgaacc tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga
    22021 tagcagggtc tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat
    22081 ttgcaaaaac atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag
    22141 cttcattatc ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt
    22201 gagctaaaaa tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa
    22261 tactgacaat atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt
    22321 tagcagcttc tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca
    22381 aaacagcttc cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga
    22441 tttcacgact aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg
    22501 tatacgcagt atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat
    22561 ttggcgaata cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat
    22621 cgatatgatt gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt
    22681 ttgcaagatt ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct
    22741 ttgctgacac gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt
    22801 aaatattcaa tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc
    22861 tcatactttc ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata
    22921 caacgatcca tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg
    22981 aggctaaaag tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg
    23041 gtgtagccat catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg
    23101 ttgccgtcaa aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta
    23161 gactgtaata ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg
    23221 ttgcgccgat tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct
    23281 gaaatagcca cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt
    23341 ctgcacctgt aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg
    23401 caaaagtagc gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct
    23461 tcaagccact catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga
    23521 aaattgagga atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga
    23581 aaaaggtcgt agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat
    23641 agcttaagaa aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa
    23701 tcaagacatt gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa
    23761 atgccagtgc tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt
    23821 cagaaaaaat cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag
    23881 tgattgcact gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca
    23941 tctttctcct cctaattatg ttcatttatt cacaataaaa aaagctttta ttttgctaat
    24001 acgtattaac atttttaatt ttatctttac attgttactt attgaacaat aggaaattaa
    24061 agaaactttt gaaacttatg taaagtgatt tttactagaa cacaaaaaaa ccttaaaacc
    24121 aaacttaaga agtttagttt taaggttaac ttaaattata ttatgcgtgg aaacgttcca
    24181 cgccatctag ataagttgct tccaatgtca tatcagggtt caatacgata aagtctgcat
    24241 cgcgtccttg agcgattttt ccacatgtat catcgatttt acagctgatt gctggaatca
    24301 gactagccat catgactgct tgttctggtg ttgctaatcc ccagtctaca acatttttaa
    24361 ttgcttcttt caatttcaat atacttccag ctaagttgcc agattctaga cgtgctgttc
    24421 cttcttttac tactactgga aattcgccta aattgtaatt tccatcaggc atcccaccgg
    24481 ccatcataca gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc attaggattc
    24541 ccgcagcatt tggatgtaca tgatggccat cacaaatcaa ttcagaaaag acatgttcta
    24601 aagataataa cgcacccacc atacctggtt cgcgatgatt caatccacgc ataccattga
    24661 aagcatggac aaatacactt gcgcctgctt caacagcttt ttgcgcttct tctagtgtag
    24721 cattactgtg tcctagagaa acaactactc cttcttcagt cacttgtttg acaaactctt
    24781 ctacaccttg acgttccggt gctaaagcaa tttttttgat taaaccacct gaagcttctt
    24841 gccattcatg gaaagtatcc aaatcaggat ccccgaaata acttgggttt tgtgcccctt
    24901 tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc tttgatttta gctcctggtg
    24961 cttcctgata cattttgccg attgtctcag ctacatcttt taaacgttct ttgcttgacg
    25021 tcaatgttgt tggtaaaaat gaagtcactc cacaagataa cagaccttca gacatcactt
    25081 tgatgccttc tgcatcatta tccattacat catgattcat atagccgtga atatgtgtat
    25141 ctaccaaacc aggagcaatc catttgccgc tttggtcaat gatgttgact tctccctcag
    25201 gtagttcttt ggtgtattca ccaaaaagtc catcggtaat ttcaagatag cctgcatgtt
    25261 tgacacctga cgctaaaaag aatttgtcag catagataaa agttctcatt ctattctctc
    25321 cctttgtttt tctacctcta aagtactctt atttagagaa gaagtcaaga atggtctaca
    25381 ccatttacaa attttttgaa atttgtttca cagcttgtgc taagccttca tagaatgctg
    25441 ctctttctgg ttcttcttta cgcttgtata ttaagctttt aagcacaaat aaatcattga
    25501 ggtaatagta gctattcttc tcttgagccc aatagattcc ttgattcacc tgttctaacg
    25561 cttcgtcgat ttcattttga tggaataaga aatttccata attataaaaa atttgcgaaa
    25621 attcactttt tgatcgttca tcagctgatt gatgcagcag ttggatgctt cgactcaaat
    25681 agtagcgtgc ttcagccgtt tttcctgcca caccatatat ttttccgata caactaatca
    25741 gttgtatttc tgtgtcagaa acatggattc tttctttttg gctagagtag cttaatgctt
    25801 ctttcaaata ataaagggac tgagagatat tttgacgcaa cgtgaattcg caaacaccga
    25861 gataataata atagcgttga aagtcagttg cttgatacag attttcagaa atatcagaag
    25921 attttagtac ttgttctaac ttccgataat cacgtttttg gaaacaacta tccaacagtt
    25981 caaataattt atcgtttgtc cgagtagttt ttcgagagag attcatcaca tgatcaatcg
    26041 ttacccctag ccgttgacaa atctgcatca tcaccaatac attcggcagt tcctcattat
    26101 tttcgatccg acttaataca ctttgtgaac aaatatcatg cgaaagcatt ttttgcgtca
    26161 ttttttgttc ttttcgaatc tctttaatga tcgttccaaa ggactcgatt aacgtttcca
    26221 taagtatcct ccccaaaata tgcgtattcg cattctattc gtattttact ttaggaagtc
    26281 gcaaacttca agaaaagact tgcaactaaa taatatactt tagttattta agaaaactcc
    26341 actagaaaga gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa tttggttttt
    26401 aaaatcattt ttggcgatag gttgctagga attctctcat tttcttagct gtttgtttca
    26461 aatcatccat tttaggaaga taaacgattc gaaaatgatc tggctggttc caattaaatc
    26521 caccaccatg tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg aatttttcat
    26581 catccaaaat attgaatcgc tttgtatcaa tttttgggaa tatataaaaa gcagcttttg
    26641 gttttacggc tgaaagacca ggaatatcat tgattgcatt atagatatat tctctttgtt
    26701 catagatcct tccgcctggc aggagcagct catcgacact ttgataaccg cctagcgcgg
    26761 tttggataat ttgctgagac aatacattgg aacacaaacg catggaggac aacatattca
    26821 gcccttctat atagcctttg acgtgtgact tatctccact taggaccatc cagcctactc
    26881 gaaatcctgc tacacgatga gatttggata gaccgttcag cgtaacaacg aacaaatcag
    26941 gcgctaatgt agcaatcgga atatgttcta gaccatccat cactaagcga tcatagattt
    27001 cgtcggaata aataatcagg ttattttgtc gagctacttc aacgatttct tctaataatt
    27061 cttttggata aagtgcccca gtaggattat taggattaat gataacaata gcttttgtcc
    27121 gactcgttat ttttgatttg atatcatcaa tatcaggata ccattctgcc tcttcatcgc
    27181 aaatataatg aacaggtgtg cctccagcta atgcaataga agccgtccac aacggatagt
    27241 ccggcattgg aaccagtact tcatctccat tgtccagcaa cccctgcata cacatcgtga
    27301 tcaattcgct cactccgttg ccggtgtaga tatcattgat cgtcacattc ggaaaatgtt
    27361 tcagttggca atattgctca attgcttttc tagcagaaaa gatccccttg gaatctgagt
    27421 agccttctga gtcacgggca ttcatgatca aatcacgaat gacttcgttt ggtgcttcaa
    27481 atccaaaagg tgcgggattt cccgtgttca attttaaaat actaatgccc tcttcttgca
    27541 tccgctccgc ttcttctaga acgggaccgc gaacatcgta actgacacct tctaatttgt
    27601 tggatttttc aaattctctc atcacttagc caatccctca tttcaaaaag ttagaacttt
    27661 ccaaattttt tgttatttac tatttcaata tcatactcca tcctagaaaa ttttgccact
    27721 tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac ttaattgtca
    27781 tgatagtcat tccatagatt cgctgcccaa tccattgcaa ttccacaaat gattcctagc
    27841 ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca atacacctaa gatcattccc
    27901 agtcctgaaa attctccgtc atatttctcc atcttaataa gtcctcccat ttttgagctt
    27961 gacaactatg taaaacctcg ctatcattag tgtgttatta ttacgcagat gtgttggaat
    28021 tggcagacaa gcatgattga gggtcatgtg agcgttagct cgtgtgggtt cgagtcccat
    28081 catctgcact acaactcgtg aagttaattt tgcttcacga gtttttttgt ttgaacgttt
    28141 ctttatgcca ttatatacat atcactgtat cctttacaaa aagaacgtac aatcatgtaa
    28201 acagcctgag gtgagtatat gaaggtcgga aacaagctac tcgtagaaac ttgcaagtgt
    28261 taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc gagggatttt
    28321 ttgtatatta tctgtttatc accgttatta taaaggtaca aataccaagc acggttaatc
    28381 caaattgcat aaattcattt ttttgtacac tcatgctttt cattagctct ttaactgcca
    28441 ttagtactaa gatggcgata acaacataaa gtatatagta cgtccgcatt ccggaagccc
    28501 atactgtggg agaaaatccc ataattaacc ttgaacaaaa tcccattatc atcaaaataa
    28561 ctaaaaaaat agaagttttc ttattgtcaa aacttaaata aattccaaca attaaacata
    28621 gtaataatgc caagactact aaagtagcta tccaagtacc tggataaaaa agtgacagtt
    28681 tcgttcctaa ctctgtaaag agattattca ggttactact actccaaatc attgcccttt
    28741 tatttccatt gacgtaagta aagctttgac ccattgtatt accaaaataa ataatcaggt
    28801 ttaagaaaaa cggtaatgca gtcaatatac gcacataata gttttgacat ttcctatatg
    28861 ttagaaggaa aattaagaaa aatagcaata aaaataagat attcatgtct aaaaaaaacg
    28921 gctttccaaa tgatgaaata cctaaatcta acttattaag aaagttgaaa tttttatatt
    28981 caggaaacca tttattaatt tcatgtgcag cacgtaatgc attccctgga gttgtcaaag
    29041 agaaaattaa ttctgctaag cttattattg aaaatactga taacttataa ttatatcgtc
    29101 cattgacaat caaatataaa ctaactatac ttgttagaac aaaaaaacag gcgttgactt
    29161 gttcttgatt agttaaaaat atcaataaag gaatacttac ccaatatatt tttctattta
    29221 tttcttctcc tcttaagagt tgaaaaaacg gataaaaagt tagtaaacta aatgctactg
    29281 gccactgata gttcaaggtg gttgctctcc aaccggtttc ccccattact gtcaatggta
    29341 ttaaacagta tatagagcaa accaataata aatttttaac atttattttc tcaaaaacat
    29401 acttgcaaag taatactaca attcctaaca taatcgtaga atttaataat ttccatagca
    29461 taaagtgttt agaaaagaac attaagaata gttctataat aattcttgaa ctccatgtct
    29521 tataatgttc tatcgaagca cccaaatagc caagttcatc taatctactt gcataaacta
    29581 cgtcatccgc tctcatagtc ggcatataca tattaaagag taaaaagata ccccaaatcc
    29641 ctattagtaa caagatcagt tctttgttat tcgttatttt tctattatgt ttgttcattt
    29701 ttatactcct tcttatattt cctaatagag tataacatag ctttaagcat tacttaaaga
    29761 taacttaaaa gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa
    29821 atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg
    29881 acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata
    29941 ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag
    30001 gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt
    30061 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
    30121 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
    30181 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
    30241 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
    30301 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
    30361 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
    30421 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
    30481 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
    30541 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
    30601 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
    30661 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
    30721 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
    30781 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
    30841 aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt
    30901 taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc
    30961 ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc
    31021 agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgagtttcta catttgtttt
    31081 ttcttgtgtc agtgttgcga tttgttgatt caaaccattt acagtatttt gtaattctga
    31141 aagttttgct tgtgcttctt gtagtcttgc tgtccaatct gcattcagat gattgatttc
    31201 atcatttttt tggttgatct catctagttt gacattgatt tcattttgtt tgctttgtag
    31261 ttgtgcatcc ttgctgacta attgactttc taagttttgt ttttctgctg ccgattgatt
    31321 tttttggttt tctaattcat ttacttttgt ttggtattct tggacatctg cctttgaatc
    31381 ttgcaatgtc tgttcttttt gtgctagtcg agcggtaaga tctgtaagcg cttgctcttt
    31441 atctgtcaat gtatgatcaa gctgtgtcag attctcattg attgtatcaa tatctgtctc
    31501 tccaccccac aaaataattt catttgcgta ccgcgtgcct gtgtagccaa ttagacatag
    31561 aaatgcaacg acaatcccac cagtaataaa tttctttttc atttgcatag ctccttaatc
    31621 caatactttt acatgctaaa agtaagtgat ctcaaacaca atgtcaagcg tttttaaatt
    31681 acatgtgtaa ttttaatata aatactatca aatactcatt taatcctcct tttttatcta
    31741 tttaaaaaat cttttttata gagaaaaatg caggttaata ctttcttaaa taaatattta
    31801 tggtgaacaa aaacaaaaaa gcaagtttta ttaaacgtaa cccttttcat ttcatagatt
    31861 actgtattta ttacaaaaaa cg
//
LOCUS       DACDKJ010000026        31843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDKJ010000026 DACDKJ010000000
VERSION     DACDKJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.026"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(152..2305)
                     /locus_tag="IV328_001371"
     CDS             complement(152..2305)
                     /locus_tag="IV328_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8937518.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2599..3447)
                     /locus_tag="IV328_001372"
     CDS             complement(2599..3447)
                     /locus_tag="IV328_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8937519.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IV328_001373"
     CDS             complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IV328_001373"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8937520.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5948..6127)
                     /locus_tag="IV328_001374"
     CDS             complement(5948..6127)
                     /locus_tag="IV328_001374"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8937521.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="IV328_001375"
     CDS             complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="IV328_001375"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8937522.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8275..10554
                     /locus_tag="IV328_001376"
     CDS             8275..10554
                     /locus_tag="IV328_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8937523.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10647..11294)
                     /locus_tag="IV328_001377"
     CDS             complement(10647..11294)
                     /locus_tag="IV328_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937524.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11473..12192)
                     /locus_tag="IV328_001378"
     CDS             complement(11473..12192)
                     /locus_tag="IV328_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8937525.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12323..13474)
                     /locus_tag="IV328_001379"
     CDS             complement(12323..13474)
                     /locus_tag="IV328_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8937526.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13691..14431)
                     /locus_tag="IV328_001380"
     CDS             complement(13691..14431)
                     /locus_tag="IV328_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8937527.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="IV328_001381"
     CDS             complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="IV328_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8937528.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14815..15408)
                     /locus_tag="IV328_001382"
     CDS             complement(14815..15408)
                     /locus_tag="IV328_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8937529.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15392..16039)
                     /locus_tag="IV328_001383"
     CDS             complement(15392..16039)
                     /locus_tag="IV328_001383"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8937530.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="IV328_001384"
     CDS             complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="IV328_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8937531.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="IV328_001385"
     CDS             complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="IV328_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8937532.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17485..18012)
                     /locus_tag="IV328_001386"
     CDS             complement(17485..18012)
                     /locus_tag="IV328_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8937533.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18192..18977)
                     /locus_tag="IV328_001387"
     CDS             complement(18192..18977)
                     /locus_tag="IV328_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8937534.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18970..19839)
                     /locus_tag="IV328_001388"
     CDS             complement(18970..19839)
                     /locus_tag="IV328_001388"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8937535.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19840..21219)
                     /locus_tag="IV328_001389"
     CDS             complement(19840..21219)
                     /locus_tag="IV328_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8937536.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="IV328_001390"
     CDS             complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="IV328_001390"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8937537.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21671..22147)
                     /gene="accB"
                     /locus_tag="IV328_001391"
     CDS             complement(21671..22147)
                     /gene="accB"
                     /locus_tag="IV328_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8937538.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="IV328_001392"
     CDS             complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="IV328_001392"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8937539.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="IV328_001393"
     CDS             complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="IV328_001393"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8937540.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="IV328_001394"
     CDS             complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="IV328_001394"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8937541.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25177..25401)
                     /locus_tag="IV328_001395"
     CDS             complement(25177..25401)
                     /locus_tag="IV328_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8937542.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25454..26416)
                     /locus_tag="IV328_001396"
     CDS             complement(25454..26416)
                     /locus_tag="IV328_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8937543.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26426..26866)
                     /locus_tag="IV328_001397"
     CDS             complement(26426..26866)
                     /locus_tag="IV328_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8937544.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27172..28137
                     /locus_tag="IV328_001398"
     CDS             27172..28137
                     /locus_tag="IV328_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8937545.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28194..28424)
                     /locus_tag="IV328_001399"
     CDS             complement(28194..28424)
                     /locus_tag="IV328_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8937546.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28510..29397)
                     /locus_tag="IV328_001400"
     CDS             complement(28510..29397)
                     /locus_tag="IV328_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8937547.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29486..30709)
                     /locus_tag="IV328_001401"
     CDS             complement(29486..30709)
                     /locus_tag="IV328_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8937548.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30726..31805)
                     /locus_tag="IV328_001402"
     CDS             complement(30726..31805)
                     /locus_tag="IV328_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8937549.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8794 a         6971 c         5488 g        10590 t
ORIGIN      
        1 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
       61 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
      121 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
      181 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
      241 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
      301 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
      361 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
      421 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
      481 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
      541 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
      601 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
      661 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
      721 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
      781 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
      841 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
      901 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
      961 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
     1021 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
     1081 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
     1141 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
     1201 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
     1261 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
     1321 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
     1381 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
     1441 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
     1501 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
     1561 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
     1621 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
     1681 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
     1741 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
     1801 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
     1861 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
     1921 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
     1981 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
     2041 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
     2101 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
     2161 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
     2221 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
     2281 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
     2341 ttgtacccaa aaacttctta ataaaaaaga aaaaatgggc ggagcaagaa attctttacc
     2401 taatttcaag ataaaatcaa ctttttgttt cacagcaatc acttaaagtt acatatccaa
     2461 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
     2521 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
     2581 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
     2641 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
     2701 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
     2761 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
     2821 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
     2881 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
     2941 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
     3001 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
     3061 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
     3121 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
     3181 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
     3241 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
     3301 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
     3361 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
     3421 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     3481 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     3541 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
     3601 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     3661 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     3721 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     3781 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     3841 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     3901 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     3961 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     4021 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     4081 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     4141 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     4201 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     4261 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     4321 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     4381 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     4441 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     4501 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     4561 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     4621 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     4681 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     4741 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     4801 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     4861 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     4921 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     4981 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     5041 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     5101 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     5161 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     5221 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     5281 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     5341 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     5401 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     5461 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     5521 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     5581 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     5641 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     5701 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     5761 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     5821 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     5881 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     5941 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     6001 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     6061 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     6121 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     6181 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     6241 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     6301 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     6361 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     6421 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     6481 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     6541 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     6601 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     6661 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     6721 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     6781 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     6841 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     6901 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     6961 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     7021 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     7081 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     7141 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     7201 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     7261 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     7321 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     7381 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     7441 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     7501 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     7561 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     7621 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     7681 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     7741 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     7801 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     7861 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     7921 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     7981 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     8041 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     8101 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     8161 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     8221 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     8281 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     8341 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     8401 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     8461 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     8521 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     8581 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     8641 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     8701 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     8761 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     8821 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     8881 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     8941 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     9001 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     9061 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     9121 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     9181 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     9241 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     9301 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     9361 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     9421 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     9481 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     9541 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     9601 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     9661 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     9721 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     9781 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     9841 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     9901 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     9961 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
    10021 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
    10081 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
    10141 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
    10201 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
    10261 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
    10321 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
    10381 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
    10441 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
    10501 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
    10561 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
    10621 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
    10681 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
    10741 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
    10801 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
    10861 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
    10921 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
    10981 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
    11041 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
    11101 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
    11161 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
    11221 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
    11281 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
    11341 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
    11401 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
    11461 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
    11521 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
    11581 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
    11641 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
    11701 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
    11761 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
    11821 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
    11881 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
    11941 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
    12001 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
    12061 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
    12121 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
    12181 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
    12241 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
    12301 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
    12361 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
    12421 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
    12481 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
    12541 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
    12601 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    12661 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    12721 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    12781 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    12841 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    12901 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    12961 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    13021 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    13081 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    13141 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    13201 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    13261 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    13321 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    13381 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    13441 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    13501 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    13561 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    13621 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    13681 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    13741 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    13801 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    13861 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    13921 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    13981 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    14041 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    14101 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    14161 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    14221 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    14281 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    14341 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    14401 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    14461 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    14521 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    14581 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    14641 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    14701 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    14761 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    14821 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    14881 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    14941 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    15001 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    15061 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    15121 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    15181 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    15241 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    15301 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    15361 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    15421 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    15481 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    15541 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    15601 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    15661 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    15721 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    15781 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    15841 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    15901 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    15961 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    16021 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    16081 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    16141 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    16201 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    16261 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    16321 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    16381 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    16441 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    16501 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    16561 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    16621 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    16681 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    16741 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    16801 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    16861 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    16921 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    16981 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    17041 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    17101 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    17161 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    17221 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    17281 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    17341 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    17401 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    17461 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    17521 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    17581 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    17641 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    17701 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    17761 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    17821 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    17881 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    17941 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    18001 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    18061 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    18121 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    18181 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    18241 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    18301 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    18361 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    18421 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    18481 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    18541 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    18601 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    18661 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    18721 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    18781 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    18841 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    18901 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    18961 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    19021 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    19081 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    19141 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    19201 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    19261 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    19321 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    19381 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    19441 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    19501 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    19561 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    19621 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    19681 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    19741 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    19801 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    19861 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    19921 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    19981 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    20041 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    20101 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    20161 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    20221 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    20281 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    20341 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    20401 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    20461 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    20521 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    20581 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    20641 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    20701 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    20761 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    20821 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    20881 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    20941 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    21001 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    21061 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    21121 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    21181 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    21241 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    21301 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    21361 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    21421 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    21481 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    21541 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    21601 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    21661 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    21721 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    21781 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    21841 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    21901 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    21961 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    22021 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    22081 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    22141 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    22201 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    22261 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    22321 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    22381 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    22441 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    22501 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    22561 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    22621 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    22681 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    22741 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    22801 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    22861 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    22921 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    22981 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    23041 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    23101 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    23161 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    23221 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    23281 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    23341 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    23401 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    23461 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    23521 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    23581 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    23641 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    23701 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    23761 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    23821 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    23881 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    23941 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    24001 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    24061 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    24121 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    24181 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    24241 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    24301 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    24361 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    24421 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    24481 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    24541 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    24601 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    24661 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    24721 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    24781 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    24841 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    24901 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    24961 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    25021 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    25081 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    25141 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    25201 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    25261 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    25321 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    25381 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    25441 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    25501 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    25561 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    25621 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    25681 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    25741 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    25801 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    25861 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    25921 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    25981 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    26041 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    26101 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    26161 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    26221 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    26281 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    26341 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    26401 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    26461 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    26521 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    26581 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    26641 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    26701 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    26761 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    26821 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    26881 taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat
    26941 gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt actcatttaa
    27001 aacaactgat aaatcagcat ttataatttc ttttattaga aaaatcatct atctcacata
    27061 ttttgattat caaagtattt tctgtgaaca gaactataga attccctaaa acttgtccat
    27121 tatttcaaaa tttgttatag tctttttatt caaagcgtaa aaggagatcc tatgttaaaa
    27181 gaaaaaatga aaactctccc catgctgccg ggtgtctatc ttatgaaaga ccgagatggt
    27241 aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag ctattttcat
    27301 cagaataaac agcattcaaa aaaagtatta cgtatgattc atcatataac agattttgat
    27361 ttcgtcgtag tagacacgga attagatgct ttattgctcg agtgtcaatt gatccaacat
    27421 taccgtcctt tttataaccg acaaatgaat tatttcagta attataatta tgtacatatt
    27481 accaataagg gatttgtatt aacagatact cctacggctc gaacatacgg gccttttcgt
    27541 ctctataaaa aaatgccgtc tattttgcgt ataatggaag aaacttatca aatgccttgg
    27601 ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc ctgacctaca ggaaatgact
    27661 tttgaacaga aaaaaaagga actccaaggg ctgtttcaag gccgaaataa aaagctactg
    27721 acttacttga aaaaaagaca acaacatttc atctatcagt tgaatttcga aaaagccggt
    27781 atgcttcaaa aagatatcga attagttact tattttatta gacggatcca ggaacaaaag
    27841 cagtttttaa gaacaccatc gctcaccttc agcatgccat tagcagcaga tgagagtcag
    27901 aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa caatgattac ctctggtgac
    27961 gtttctcctg acttctatta tgaaaagaaa gaagctcatt tatctttgaa acgtcaattg
    28021 tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt accgcaaaaa attagaaaaa
    28081 gaacagtttg aaattgaact gttaaacaaa aaagaagctg aaaagcaact aaattagttg
    28141 cttctcggct tcttttaggc atcttcaact tcaaatgtca attatcctaa aatttattgg
    28201 attaagtcat agatttccat cgctacgata tcgatattat caaattcgta ttgctgatga
    28261 gtatctgttt cagttaattc aaatgtatcg gtcgctttat cataagttac tgtacacttc
    28321 tctacgcctt ctttttcaaa acggcgaact tgcagttcat tatctgctga ctcgaccata
    28381 gcctccagtc gttttatgat tgcgactaat tgtgaatgtg tcatcggagg tcctcctttt
    28441 cttatttctg ttatattgta tcaaaagcat agcctacttg cattgatttt ctctaaaaaa
    28501 actcgttttt tatgattttt tttctggtat ttttccttca ggccaccaaa gtttgatcaa
    28561 agcttgggtt ccgtcattct caaaaccttt atctgccaac tcttcatata gtttcaatgc
    28621 ttgggtagtt gctggcaata caagatccat tttttctgct tcttctaatg caatctttaa
    28681 atctttgata aaatgtttga caaaaaaacc aggtgtgtaa tcttctttta aaatacgagg
    28741 agaataattg cttaaagacc agttagctgc acttccacct gaaagcgtct caatcacttt
    28801 ctttaaatca agtccgtttt tttgtgcata gacaagcatt tccgtcattc ctgtcatcgt
    28861 acctgcaatc atgatctgat tagccatttt tgtatgctga cctttcccag cagaaccatg
    28921 caatgtaaat gtcgtaccaa attctttgaa taaaggcagt acttgatcat aaacggcttc
    28981 ttctccaccg accatgatcg tcagtgtacc attctttgct cctaagtcac caccggatac
    29041 cggtgcatct aacgcatctg cgcctttttc ttttgctgtt tttgcaattt tttctgccag
    29101 gcttggtgta cttgtagtca aatcaactaa aatatgtcct gagatatctg cagaaaaaat
    29161 ccccgactgt ccatagtaga tttcttctac atctttgggg taacctacca ttgtaaagag
    29221 aatattactt tgttctgcta ccgcttttgg tgtatctgcc cagatagctc cttctgttac
    29281 taaaggatca gcttttgctt ttgtccgatt atagacagct acttcgtgtc ctgcttctag
    29341 tagatggcgt gcgacagcac ttcccatgac gcctgttccg ataaaaccaa gtttcatttt
    29401 ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta gtatacgcta gaaacaagga
    29461 ttttttcata aaaaatgttt gaattttatc ttttaaatga atttaatttt attttgattc
    29521 gttcaatcga gtcttcttct tcttttcttc gcatctcttc ttcatgataa acaaccatgt
    29581 cttgataaaa ttcgatcata gcttcgccga aatgtgtaga tgaaatatca taaagtttag
    29641 cttctaaaac ttttggatct ttaggaactt cactggaaat ataatcaata agtgctggtg
    29701 caaagtcttc gtcggtttca aaagttttcc ctaatgaagg atgatcgaac aatcggttca
    29761 aataagcatt tccttccacg acacaaggtg ttccggctgc catcgcttct gtataagtaa
    29821 gcccttgtgt ttctgaagta gaagcactga caaaataatc tgccgcttta taataaagtg
    29881 ctacatcatt gttgtccact tctcctgtga actgcacaaa ttcttctaac tgcagttctg
    29941 agatcaattc ctctaactct tctacataag gtccttttcc tacgataact aatctggcgt
    30001 taggataagc ggagatgacc tgaggaaacc cattaatcaa ggcttggata tttttctcat
    30061 aagaaatcct gcttaaagaa agaatcatca aatggttttc ctgaagacct agtgactccc
    30121 ttaatttgct gatatcctgc tgagtgatat cagaacgttt gaatttttca atgtcgatac
    30181 cagtaggaat cacgcgaagc ggtgctatta caccatagct gcgtaatgta tcaataacac
    30241 gttcacttgg acaaactaca cctgtcgtat gatttgcaaa taatctagac aagtatttta
    30301 catgggattg cctgatcact tttcctttac cgatataatg aaggtagtct tcatacatcg
    30361 tatggtaagt atggatcaga ggtattttca acttcttagc taccatcttc ccaagtatcc
    30421 ctgctccgaa ttctgtatgg gtatgaataa gatctagttc tagttctttg gcaaccaaat
    30481 aagcatacca catgcctcta acaacaactc ttcgatcctt gaaagaaata aatggtacac
    30541 ttggcattcg aatcacatct ttttccaact tatccgcatt aggatcggtc gttgtaaaaa
    30601 tatagacttc atgcccatgt ctttctaatt cttgttttaa tgttttgatc gatgtagcaa
    30661 ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc gattttcatg tttttcgcct
    30721 ccaaactagt ccttgacctg atttactttt tttaatttgc tagagacttt ttcattcagt
    30781 acactttcgt atacattttt aagttcttca ccgattttat tgatacttcg ctcttttgct
    30841 gtttgataac cggcttgtgt cgtactcggt agttcttgat tggtaatctg ttggatcagt
    30901 cggataaatt cttcgtttgt cttccccata tagcagttct tgctatcttc taaccagcca
    30961 ttatacactg gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc ttctaaaaca
    31021 acgatccctt ctgtctcttc ataagaaggg aaaaagaata gatctgcacc tgaatacgca
    31081 ccttctatga tctctccacg aatgtaacca ggaaacagga cattttcagg atgatcttct
    31141 tttacaattt tccggatgtt ccgaggaatc gagtacatgg gaacatgacc aaaccaaata
    31201 aatgtgtatt cgggcatctt cttagcaatt tctacgaaat caatgattcc tttacgttcg
    31261 aaaaacaaac cgacacaaat gattactttt tgatccggag acagtttgaa atattctctg
    31321 aacttttgtt ctttcattgg atcagggtga tatttctcta agtcgatccc attggaaata
    31381 gcttgaatcg gcacacggat accatagctt tccaacaact gttttgaata aggtgttggc
    31441 gtgatcagat agtccgcttt ttggtacaat cctaccagat atttttttac gataggagaa
    31501 agctggttcg atccgataaa ggaatttcgg aaatcttcct ctgtagaatg cgcatgatag
    31561 acaacttttt ttccttctct tctggcttta ttgaccatat tatgactatt gattccgtat
    31621 gtattgatgt gcaggatgtc ataatcttct ttttcatcta gtgtataaga gataccgact
    31681 tccgtcagag cacgtttttg atgatcaagc gcacgcccga ttccagattt tgcaagcatc
    31741 ttttcacttt caaaatataa taataccttc accttacgtc ccccttcact tatatagtat
    31801 aacatagcct acaaactgtc actcaaactt aagccctatt ttt
//
LOCUS       DACDKJ010000027        31246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDKJ010000027 DACDKJ010000000
VERSION     DACDKJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.027"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IV328_001403"
     CDS             202..1077
                     /locus_tag="IV328_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937550.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IV328_001404"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IV328_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IV328_001405"
     CDS             complement(1642..2973)
                     /locus_tag="IV328_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8937551.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="IV328_001406"
     CDS             complement(3122..3835)
                     /locus_tag="IV328_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8937552.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="IV328_001407"
     CDS             4010..5434
                     /locus_tag="IV328_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8937553.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="IV328_001408"
     CDS             5733..6440
                     /locus_tag="IV328_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8937554.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="IV328_001409"
     CDS             6427..6750
                     /locus_tag="IV328_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8937555.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="IV328_001410"
     CDS             complement(6839..7876)
                     /locus_tag="IV328_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8937556.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="IV328_001411"
     CDS             complement(8197..9180)
                     /locus_tag="IV328_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8937557.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="IV328_001412"
     CDS             complement(9595..10365)
                     /locus_tag="IV328_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937558.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="IV328_001413"
     CDS             complement(10358..11293)
                     /locus_tag="IV328_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937559.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="IV328_001414"
     CDS             complement(11609..13258)
                     /locus_tag="IV328_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8937560.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="IV328_001415"
     CDS             complement(13375..15978)
                     /locus_tag="IV328_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8937561.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="IV328_001416"
     CDS             complement(15990..17039)
                     /locus_tag="IV328_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8937562.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="IV328_001417"
     CDS             complement(17065..17379)
                     /locus_tag="IV328_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8937563.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="IV328_001418"
     CDS             complement(17395..17841)
                     /locus_tag="IV328_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8937564.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19818)
                     /locus_tag="IV328_001419"
     CDS             complement(17842..19818)
                     /locus_tag="IV328_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8937565.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="IV328_001420"
     CDS             complement(19976..20872)
                     /locus_tag="IV328_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8937566.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="IV328_001421"
     CDS             complement(20865..21545)
                     /locus_tag="IV328_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8937567.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="IV328_001422"
     CDS             complement(21546..22328)
                     /locus_tag="IV328_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8937568.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="IV328_001423"
     CDS             complement(22321..23238)
                     /locus_tag="IV328_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8937569.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="IV328_001424"
     CDS             complement(23402..23725)
                     /locus_tag="IV328_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8937570.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="IV328_001425"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="IV328_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8937571.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="IV328_001426"
     CDS             27853..28167
                     /locus_tag="IV328_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8937572.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="IV328_001427"
     CDS             28180..28554
                     /locus_tag="IV328_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8937573.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="IV328_001428"
     CDS             28564..28899
                     /locus_tag="IV328_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8937574.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="IV328_001429"
     CDS             28982..30322
                     /locus_tag="IV328_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8937575.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="IV328_001430"
     CDS             30400..31074
                     /locus_tag="IV328_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8937576.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
BASE COUNT         9737 a         6069 c         5515 g         9925 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaata
//
LOCUS       DACDKJ010000028        30984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDKJ010000028 DACDKJ010000000
VERSION     DACDKJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.028"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..207)
                     /locus_tag="IV328_001431"
     CDS             complement(61..207)
                     /locus_tag="IV328_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP8937577.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(311..622)
                     /locus_tag="IV328_001432"
     CDS             complement(311..622)
                     /locus_tag="IV328_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8937578.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(624..821)
                     /locus_tag="IV328_001433"
     CDS             complement(624..821)
                     /locus_tag="IV328_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8937579.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1215..2942)
                     /locus_tag="IV328_001434"
     CDS             complement(1215..2942)
                     /locus_tag="IV328_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8937580.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2935..4128)
                     /locus_tag="IV328_001435"
     CDS             complement(2935..4128)
                     /locus_tag="IV328_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8937581.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4315..4959)
                     /locus_tag="IV328_001436"
     CDS             complement(4315..4959)
                     /locus_tag="IV328_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8937582.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5053..5187)
                     /locus_tag="IV328_001437"
     CDS             complement(5053..5187)
                     /locus_tag="IV328_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8937583.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5189..7321)
                     /gene="feoB"
                     /locus_tag="IV328_001438"
     CDS             complement(5189..7321)
                     /gene="feoB"
                     /locus_tag="IV328_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8937584.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7318..7791)
                     /locus_tag="IV328_001439"
     CDS             complement(7318..7791)
                     /locus_tag="IV328_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8937585.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8192..8416
                     /gene="nrdH"
                     /locus_tag="IV328_001440"
     CDS             8192..8416
                     /gene="nrdH"
                     /locus_tag="IV328_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8937586.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8575..10734
                     /gene="nrdE"
                     /locus_tag="IV328_001441"
     CDS             8575..10734
                     /gene="nrdE"
                     /locus_tag="IV328_001441"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8937587.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10784..11749
                     /gene="nrdF"
                     /locus_tag="IV328_001442"
     CDS             10784..11749
                     /gene="nrdF"
                     /locus_tag="IV328_001442"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8937588.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11838..12977)
                     /locus_tag="IV328_001443"
     CDS             complement(11838..12977)
                     /locus_tag="IV328_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8937589.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13286..13999)
                     /locus_tag="IV328_001444"
     CDS             complement(13286..13999)
                     /locus_tag="IV328_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8937590.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14253..14954)
                     /gene="nagB"
                     /locus_tag="IV328_001445"
     CDS             complement(14253..14954)
                     /gene="nagB"
                     /locus_tag="IV328_001445"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8937591.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15188..16720
                     /locus_tag="IV328_001446"
     CDS             15188..16720
                     /locus_tag="IV328_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8937592.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16911..18113)
                     /locus_tag="IV328_001447"
     CDS             complement(16911..18113)
                     /locus_tag="IV328_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8937593.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18195..18884)
                     /locus_tag="IV328_001448"
     CDS             complement(18195..18884)
                     /locus_tag="IV328_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8937594.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18990..19358)
                     /gene="rplL"
                     /locus_tag="IV328_001449"
     CDS             complement(18990..19358)
                     /gene="rplL"
                     /locus_tag="IV328_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8937595.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19411..19914)
                     /locus_tag="IV328_001450"
     CDS             complement(19411..19914)
                     /locus_tag="IV328_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8937596.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(20324..21013)
                     /gene="rplA"
                     /locus_tag="IV328_001451"
     CDS             complement(20324..21013)
                     /gene="rplA"
                     /locus_tag="IV328_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8937597.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21117..21539)
                     /gene="rplK"
                     /locus_tag="IV328_001452"
     CDS             complement(21117..21539)
                     /gene="rplK"
                     /locus_tag="IV328_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8937598.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21882..22550
                     /gene="sdaAB"
                     /locus_tag="IV328_001453"
     CDS             21882..22550
                     /gene="sdaAB"
                     /locus_tag="IV328_001453"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8937599.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22563..23435
                     /gene="sdaAA"
                     /locus_tag="IV328_001454"
     CDS             22563..23435
                     /gene="sdaAA"
                     /locus_tag="IV328_001454"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8937600.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23526..24545)
                     /locus_tag="IV328_001455"
     CDS             complement(23526..24545)
                     /locus_tag="IV328_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8937601.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24718..25605)
                     /locus_tag="IV328_001456"
     CDS             complement(24718..25605)
                     /locus_tag="IV328_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8937602.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25682..26227)
                     /gene="nusG"
                     /locus_tag="IV328_001457"
     CDS             complement(25682..26227)
                     /gene="nusG"
                     /locus_tag="IV328_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8937603.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26328..26498)
                     /gene="secE"
                     /locus_tag="IV328_001458"
     CDS             complement(26328..26498)
                     /gene="secE"
                     /locus_tag="IV328_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8937604.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26522..26674)
                     /gene="rpmG"
                     /locus_tag="IV328_001459"
     CDS             complement(26522..26674)
                     /gene="rpmG"
                     /locus_tag="IV328_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8937605.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26836..27480
                     /locus_tag="IV328_001460"
     CDS             26836..27480
                     /locus_tag="IV328_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8937606.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27536..28039)
                     /locus_tag="IV328_001461"
     CDS             complement(27536..28039)
                     /locus_tag="IV328_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8937607.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(28052..28098)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28636..29601
                     /locus_tag="IV328_001462"
     CDS             28636..29601
                     /locus_tag="IV328_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8937608.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29700..30872)
                     /locus_tag="IV328_001463"
     CDS             complement(29700..30872)
                     /locus_tag="IV328_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937609.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30859..>30984)
                     /locus_tag="IV328_001464"
     CDS             complement(30859..>30984)
                     /locus_tag="IV328_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937610.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9460 a         6349 c         5450 g         9725 t
ORIGIN      
        1 ttttgtatcg atagtaatca agagatcccc tcctgatttt aggatatcat aaaaaaaatt
       61 ttagttacac tttttaaata atgaaaaaac acattccact aaatttgatg ccggttttcc
      121 ttgaggtttt gttccagcac ctcctttaat ttttttaagc tcttgatctg aacattgttt
      181 agcatttagt ctattttttt cttccattac gattccccct acaaactata aatactttta
      241 tgtcactgat tcaatatacc tatttttcta atcaaaaaat tttgagacat atttaatatt
      301 ataagtatca ttaaaattga gatttatctc cataatctgc tcgatataaa ccatttattc
      361 cagcagttct tccaatttca ctcaaatttc ttattaattc ctcttgatat tcagtgaatc
      421 ttattttatt agtataagca gtaaaatata gataattcac caatctattt attaaaggac
      481 cgggatctaa ttcatatttt tgtttttcta attttaaata taccttttgt aaaacctcaa
      541 gaatatcaga atatttagga tctttactta tagatatatc attatataat tcatgaacaa
      601 tttgcttagc attttttttc attttagcac ttccctggaa ttgctccacc taaaaaacca
      661 cctatagaca ttcctgcaat acaagtagtt gccttggccc aatcgaccgt acatttattt
      721 ttagtgcaat acactccatt tccataatat tttccactat gagtggtacc cccatagata
      781 agttgtgtct ctttaataga caaaattttt aaatgtttca taatctaatc acccctaaat
      841 taatgttttt agactattat tctaacattc attaaatttc aaaaaacaat tccttcttga
      901 aacgtagcaa aataattcct gaataagaat ttaatcactt ttaagtatat aaactatgta
      961 tattctttaa tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc
     1021 tttcatacct acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc
     1081 gtctttggac gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag
     1141 agacgaacat ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt
     1201 tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag
     1261 tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata
     1321 tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc
     1381 aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct
     1441 tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca
     1501 attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat
     1561 gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta
     1621 actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca
     1681 tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa
     1741 ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta
     1801 ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt
     1861 gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact
     1921 acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt
     1981 tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca
     2041 ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta
     2101 ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat
     2161 gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg
     2221 cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt
     2281 gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat
     2341 agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact
     2401 tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca
     2461 aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat
     2521 cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt
     2581 tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga
     2641 acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat aaaccataga
     2701 ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc
     2761 gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac
     2821 atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac
     2881 cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc
     2941 atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac
     3001 accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt
     3061 gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc
     3121 gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt
     3181 caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt
     3241 caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt
     3301 ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt
     3361 tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat
     3421 caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat
     3481 cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag
     3541 cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc
     3601 tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt
     3661 aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt
     3721 aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa
     3781 cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc
     3841 tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat
     3901 cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac
     3961 gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt
     4021 tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt
     4081 ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct
     4141 tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt
     4201 agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa
     4261 tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc
     4321 tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc
     4381 aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag
     4441 ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc
     4501 gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa
     4561 aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa
     4621 atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg
     4681 gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac
     4741 aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc
     4801 cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat
     4861 cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg
     4921 ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt
     4981 tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct
     5041 tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc
     5101 atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat
     5161 taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat
     5221 aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta
     5281 aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat
     5341 aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc
     5401 atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa
     5461 gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt
     5521 gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc
     5581 caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc
     5641 cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt
     5701 ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac
     5761 tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt
     5821 gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag
     5881 ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag
     5941 tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg
     6001 ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg
     6061 tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac
     6121 atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa
     6181 gtcgatccat gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc
     6241 ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga
     6301 tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc
     6361 caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg
     6421 ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca
     6481 aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag
     6541 aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga
     6601 ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga
     6661 tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa
     6721 aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt
     6781 catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct
     6841 tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc
     6901 tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc
     6961 cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca
     7021 ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga
     7081 tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag
     7141 gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg
     7201 atccttcttt tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag
     7261 tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca
     7321 ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc
     7381 ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat
     7441 ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg
     7501 gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc
     7561 agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac
     7621 tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt
     7681 acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat
     7741 tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc
     7801 tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt
     7861 gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca
     7921 attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa
     7981 tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat
     8041 acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca
     8101 caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga
     8161 taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa
     8221 ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga
     8281 agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag
     8341 tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg
     8401 taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct
     8461 tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat
     8521 gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt
     8581 cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc
     8641 aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat
     8701 gtcaaaccaa atacgatgac ttttctatct attatggaaa aaatcaatta cttgatcgat
     8761 caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta
     8821 tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt
     8881 tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat
     8941 cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt
     9001 gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga
     9061 cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac
     9121 tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg
     9181 attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca
     9241 agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta
     9301 ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct
     9361 ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt
     9421 ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta
     9481 tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca
     9541 gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt
     9601 gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat
     9661 attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt
     9721 tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta
     9781 ttaggaacgg atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc
     9841 cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca
     9901 tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga
     9961 ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag
    10021 gattcattag actttacaaa tatttacttc atgctgttga attattggac acttgttgaa
    10081 agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat
    10141 gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa
    10201 gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa
    10261 caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc
    10321 tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa
    10381 gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag
    10441 acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat
    10501 gcaatggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa
    10561 atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat
    10621 attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt
    10681 actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat
    10741 agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta
    10801 tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct
    10861 gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag atgattggcg
    10921 tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct
    10981 agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca
    11041 tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc
    11101 ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa
    11161 tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac
    11221 accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt
    11281 ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa
    11341 actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg
    11401 attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct
    11461 ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg
    11521 gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg
    11581 gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat
    11641 ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg
    11701 tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt
    11761 aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac
    11821 tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt
    11881 tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc
    11941 ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct
    12001 gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac
    12061 aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac
    12121 aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa
    12181 gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa
    12241 ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag
    12301 atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac
    12361 tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc
    12421 ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata
    12481 aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta
    12541 ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc
    12601 cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg
    12661 gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact
    12721 aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt
    12781 tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt
    12841 aaaaaaactg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata
    12901 aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt
    12961 ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta
    13021 acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat
    13081 tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata
    13141 ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca
    13201 gccaaacgaa aaatataagg aactattttt atttggctgg gcttattact tgatgtagga
    13261 catctatttt acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga
    13321 tatttttgtg tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat
    13381 caatcgtata gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca
    13441 catcttcgat tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt
    13501 ttctatggat aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca
    13561 ctactgtgag tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca
    13621 tgccgatggc gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg
    13681 tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca
    13741 cttgagcaat cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta
    13801 aagcatctct taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc
    13861 ccatacaaac taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa
    13921 aaccgattgc tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg
    13981 tcaataccat atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc
    14041 agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc
    14101 atagtccttt ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa
    14161 cggcaaaata tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg
    14221 atttcacaac ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg
    14281 atgatcacaa caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca
    14341 ggtccttcaa tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg
    14401 atttctttac ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct
    14461 tctgctttat cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact
    14521 gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg
    14581 attcctaaga tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat
    14641 gctgcttcca gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta
    14701 tcaaataatt ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca
    14761 acgtactcat ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt
    14821 tctttatata aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg
    14881 ttcatacctt ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca
    14941 cgaatgattt ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct
    15001 ttttttcttt ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa
    15061 taaataggaa tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg
    15121 aaaaccaagc aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg
    15181 agttattatg agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct
    15241 ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa
    15301 cggagccggt aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg
    15361 acagatccat tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga
    15421 tgaaacatta acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga
    15481 agaaaaaatt gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga
    15541 aaaagctggg aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt
    15601 agttgctgag atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa
    15661 aaatctaagt ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc
    15721 agatgttctg atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct
    15781 gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt
    15841 tcttgatcag atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac
    15901 tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca
    15961 atatcatgct caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc
    16021 cggttcccga tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct
    16081 gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac
    16141 gacattagca gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc
    16201 catcaatttg accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg
    16261 aaaatctact ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca
    16321 cttcccagat aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt
    16381 agaaacacca ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg
    16441 tcgccaacta gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt
    16501 aagtggtggg gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt
    16561 actgattctc gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga
    16621 cgggatacag aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca
    16681 agatcttgtg caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga
    16741 aaaaaaggag ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca
    16801 attagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt
    16861 ggctattctt attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta
    16921 ttttcgagat ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga
    16981 aaaacaagcc cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct
    17041 agcaatgaac cagccgcacc tccaataaag tacataaaca taaatacagt attatttcgg
    17101 ttgcttgctg cttcacctag cccttgtact cgtgtttgat tggcgacttg accaaattga
    17161 ttcccgacgt ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc
    17221 aataaagcaa taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca
    17281 gaataagaat ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa
    17341 atcgctaata tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa
    17401 atcaacgtag accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct
    17461 tctcttaata gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat
    17521 gaaacagctg tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta
    17581 agaattccga ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca
    17641 acaataccag ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta
    17701 cccatgacct tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt
    17761 tgcggcacga tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa
    17821 ggcgcaaaaa aagcggccaa cagagagacg gcagataacg ctgccattcg aatgatcaat
    17881 tttggacgat ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct
    17941 aattgggtca acatcgtcaa tatcccgact gcacttgtgc taacagataa actagctgct
    18001 acttttgttc caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt
    18061 gataatagta aaataagcga agttgttaaa ggtttttttt cattactctt cattgatttc
    18121 ccccatttcc tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact
    18181 attcgccaca cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa
    18241 atgaagacaa ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg
    18301 ttatatccgt gaaacagagc ttctccacca cacgcataca acacagacga agggatgact
    18361 ccaatcacga tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa
    18421 ttgaccatag aagatggaat aaccggtatc atatagccta acattacacc tatttttgga
    18481 tgtttcattt gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat
    18541 tttaagcgat gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg
    18601 atgatcgcac ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta
    18661 ggaatacctg gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa
    18721 gtcccatgcg aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat
    18781 aattggatgt ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg
    18841 attcccaacg agatcaatat caatcggatt attttctttt tcatatttcc tcctgaaaaa
    18901 aatctatatt cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat
    18961 aggatctcag tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct
    19021 tcaagtttag ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca
    19081 ggagcgccat caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact
    19141 gctttgataa ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt
    19201 tgttcttcag cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat
    19261 acgccaaatt cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct
    19321 tttaactcag caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg
    19381 ttctttttta tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc
    19441 cactgctttg acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat
    19501 agatagaagt ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga
    19561 gactttacct tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc
    19621 gtccatgatt ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc
    19681 agtgaatact tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga
    19741 atttttgata actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac
    19801 ttcatcaact gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt
    19861 ttcgcttact tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga
    19921 gtttcacctc ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta
    19981 tagttacttt gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta
    20041 ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc
    20101 ttcacttcgt tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga
    20161 ctattgaata tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg
    20221 tcttcggtac aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca
    20281 agaattattt ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt
    20341 caacatggat accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt
    20401 tagctgctga tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta
    20461 atttttcatc gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca
    20521 cacggtaagt tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa
    20581 ctgtaccagt tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac
    20641 ctacagtagc catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt
    20701 ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt
    20761 ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta
    20821 atacaacagc accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg
    20881 caacttcaac tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt
    20941 cggcaacagg ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc
    21001 tctttttagc cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg
    21061 ttctatatct ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat
    21121 tctacagtga tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg
    21181 ttagctgcgt ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa
    21241 actttcgcaa ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt
    21301 ttcaatagaa cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat
    21361 acagaaataa caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct
    21421 tttgtgaatc ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct
    21481 ggagttgctt tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg
    21541 agacatacct ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg
    21601 ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag
    21661 tatagcaaat ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa
    21721 taaacgctaa atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt
    21781 tacacttgcg ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc
    21841 cttgtacaaa gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata
    21901 gaagtgtctt tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg
    21961 gagctgctag aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag
    22021 atatttatct atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat
    22081 tagttggcgg cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat
    22141 tggcatatga agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc
    22201 caaactctgt aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt
    22261 cgatcggcgg tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca
    22321 tgggtactcc taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga
    22381 ctagtatact ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa
    22441 aaggtgaaaa tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa
    22501 aaagactcaa acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa
    22561 aaatgttttt atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag
    22621 aattgatgat tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga
    22681 tcatggaaaa aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta
    22741 cctccgtcac aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg
    22801 cagacttttt aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg
    22861 aagtcaatgc aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg
    22921 ttcctggtgt gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg
    22981 aattcttatt taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg
    23041 gtgcagaagg gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt
    23101 tggtttgtgc aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga
    23161 aaaatatgat gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca
    23221 aacgtaatgc tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta
    23281 tcgaaagtgt cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa
    23341 tgccgtcgat tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac
    23401 gacttgcgga agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta
    23461 agagattagt tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt
    23521 ttttattaat ccatcaattt cgtatttttt acttcatcga tcgtttttgt tcctgcaagt
    23581 tgcatcgtga tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc
    23641 ccacctagat tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt
    23701 gccagagctt tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca
    23761 cgtttgttga ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct
    23821 agttgacgtc caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt
    23881 actgcatctt ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg
    23941 attgttttga tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt
    24001 ccttttcctt cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt
    24061 ggtaatggaa attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa
    24121 tctgccgtta aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca
    24181 ttgaatctgt cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca
    24241 tcggctgcgt ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca
    24301 gcagctactc ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct
    24361 gatggcgctt ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg
    24421 gctgagtcga ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta
    24481 ttttctttca tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct
    24541 tccatgcgct cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt
    24601 tcttcattac ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat
    24661 aaatcatact aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta
    24721 ttctttccaa ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact
    24781 atgagaggca ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa
    24841 caacgcatag accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc
    24901 atctagttga ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg
    24961 ctggtaatct tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag
    25021 agactgcgca ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg
    25081 aacttgtggc aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc
    25141 tcccatggaa tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa
    25201 aagacaagtt ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa
    25261 tacttggacc atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc
    25321 atctgttcct actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg
    25381 caacatccct ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat
    25441 agtggttttt tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt
    25501 agtatttatc ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc
    25561 tgctgcagtt gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc
    25621 catatagaaa aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt
    25681 attaatcgat tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca
    25741 tatcgatgtt gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta
    25801 atccagcaaa tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat
    25861 tttgtcgcag gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg
    25921 gtgctggttt gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc
    25981 ggacaacata ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg
    26041 ttttatgaac gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa
    26101 caacgacacg gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg
    26161 cttttacttt gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag
    26221 attccattga aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt
    26281 tttctttcgc ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc
    26341 cagccaaata agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata
    26401 gaagtttcga tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact
    26461 tgtttgattt catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg
    26521 tttatttcgt ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt
    26581 ccaaacgttc gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa
    26641 cggaacaagc gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc
    26701 agacttatac ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact
    26761 atctattgtt ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca
    26821 attggaggta tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc
    26881 gttacagaga ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt
    26941 tgtgtgccga tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat
    27001 atctatcgtc ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa
    27061 aacgctacaa agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa
    27121 gagcttcctt atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat
    27181 agtacgttgc ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg
    27241 acaattgctt ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa
    27301 cacagcagca ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt
    27361 acattgatca cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac
    27421 ttagagaaaa agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa
    27481 tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga
    27541 cacgcttttc aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca
    27601 tgacgataaa ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg
    27661 ttggaactaa catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata
    27721 ctaaacgcat ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa
    27781 gcaatgtttg gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat
    27841 agacgatgac cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat
    27901 tcaaactttc tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag
    27961 ttaacgctag ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc
    28021 tgcttttcgt tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg
    28081 ttgatgaacc gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca
    28141 atccgtctta tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa
    28201 tcgtgggata tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg
    28261 aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg
    28321 aatttgagca acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca
    28381 tgccgaacct tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt
    28441 tgtgtttttc ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga
    28501 attaattttg taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa
    28561 agcttgctaa ttattggtta accagcaaac aggagtatta aaatatcttg ttttattgaa
    28621 tagcaggagg aaatcatgaa attccattat catatattga tcaatccatc ggcaggcagc
    28681 ggaaatggct ataaagtagc agaaagaatt ttaccggtat taaaaaataa gcatattgac
    28741 tatacacttc attatagtga atacaaagga cacgatgctg agattgctga aactttggca
    28801 aaagaaacat tgattccttg gatagaagaa gaacagaaaa caatcgatac tttccccctt
    28861 ttgatcatcg taggaggtga cggcacctta catcaggtac ttgatacttt ctatcagatg
    28921 gaagtagaat tcccagttgc ttatattcca gcaggttctg gagatgattt tgctcgaggg
    28981 gcaggccttc caaaaaatcc caaaaaagga cttcagctga ttttagcagc tcaatcacca
    29041 gaaaaagtcc acataatggc ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt
    29101 aataatttcg gtataggatt agatgcagct atcgtccatg cgacaaatca ttcttccaca
    29161 aaaaagcgat tgaacaaata caacttagga tcattttcgt atttattctc tattctccgt
    29221 gctttattca ctcaaaaagg atttccaatt ctggtagatg caggaggaaa acaattaagt
    29281 ttttcaaacg catttttatg tacagcaacg aagcatcctt tttttggcgg aggaatcgcg
    29341 attgttccaa ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc
    29401 aatttcttta aaatcttgtg gctcattctt ttacttatac agaaaaaaca attgaaatcg
    29461 aaatatttcc atcactacac aacaaacaga ttacgtatcg tttcaacaac accgcaacat
    29521 ggtcaagaag atggagaaga aatggaaaaa cgaccttttg atatcacaat aaccacaact
    29581 aaacagttgt tttggtgtta ataaaagagg ttttgtcaga agctacatct ggaacaccgt
    29641 ttattcggaa aaaaggactg tgacacgact tttgtcacag tccttttttt actatgcagt
    29701 taattctttg cccaattggg ttgccatcga tagtttcttt ctttacccaa aataaattgg
    29761 atcattgaaa acaccgcagc aaaagcaact aagacacaac cattgaacca aaaagattct
    29821 gcaaatgttt gattgtaact gagtgtttca ctgatcaagt tattattttg aacaaaccaa
    29881 tatgttggtg tgaatgccgc tattttgcta accatttctg gcaagtattc tgctggtaca
    29941 aacaccccac cgataaagca actgcctgtg ataaaaatat ctttgatccc accgctggtt
    30001 tctgagtttt ttaccaatga agtgatacaa gccgaaaagc taatgatcgg caataaaaac
    30061 aagaaagtat ttaaaatatg ataccatacc gttatatccc acgaggtgtg gatcactaac
    30121 agaaccatta gaagtaagaa gccactgaac aacagcagcg agtaactaat caaactaaag
    30181 gttactttgc gattcaagtt tcgtttggta atgggcgcac tctggtttcg tttactgatc
    30241 tctttgcgat taaatgctag attgatgacg ctgaaaccgc taaaaatgga tagaaacaac
    30301 ccataagaca agaaattaaa ggttgtgccc gtcaacagct ttttcatccg ctgactatat
    30361 cccgcactta gctcaacttc tccagtaacc gagagtgcca tttgtgtctg ttccatagct
    30421 gcatcgattg ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact
    30481 tgctgtgtaa caactgtttt agaaaaggca tctgggcgac tctgtatctt gatcgtcggc
    30541 atctttccta cttcgacttg ttcggtaaag tctgcaggca gatgtaagat aatgtttaca
    30601 cgattaaaat aaagagcatc atcgatttct ttttgtgagg ttccatccaa tgtaacgact
    30661 gtttccttgt cattcaaata tttacgaagg ccatcgacta caggcgactc ttcttcacta
    30721 atcaaggcga ttttcgcatc ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa
    30781 aagaatgtgg caacaacagt gataattaca cctatcagta aattcgattt atttgcttta
    30841 agcaatgtcc aaatcgtttt ataaagcgtc ataacgcacc cctctttcca tccaagcatt
    30901 tcctaacgca aaaaggaaaa taaatccgat caaccataga agattggtat aaaaactagc
    30961 caaatcagtg tagaaataaa gttt
//
LOCUS       DACDKJ010000029        30934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDKJ010000029 DACDKJ010000000
VERSION     DACDKJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.029"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(147..773)
                     /locus_tag="IV328_001465"
     CDS             complement(147..773)
                     /locus_tag="IV328_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937611.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1068..1403)
                     /locus_tag="IV328_001466"
     CDS             complement(1068..1403)
                     /locus_tag="IV328_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8937612.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1390..1674)
                     /locus_tag="IV328_001467"
     CDS             complement(1390..1674)
                     /locus_tag="IV328_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8937613.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1730..3253)
                     /locus_tag="IV328_001468"
     CDS             complement(1730..3253)
                     /locus_tag="IV328_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8937614.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3246..4421)
                     /locus_tag="IV328_001469"
     CDS             complement(3246..4421)
                     /locus_tag="IV328_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8937615.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4425..4610)
                     /locus_tag="IV328_001470"
     CDS             complement(4425..4610)
                     /locus_tag="IV328_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937616.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4576..6270)
                     /locus_tag="IV328_001471"
     CDS             complement(4576..6270)
                     /locus_tag="IV328_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8937617.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6267..6740)
                     /locus_tag="IV328_001472"
     CDS             complement(6267..6740)
                     /locus_tag="IV328_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8937618.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6809..6964)
                     /locus_tag="IV328_001473"
     CDS             complement(6809..6964)
                     /locus_tag="IV328_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP8937619.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7098..7478)
                     /locus_tag="IV328_001474"
     CDS             complement(7098..7478)
                     /locus_tag="IV328_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8937620.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7482..7697)
                     /locus_tag="IV328_001475"
     CDS             complement(7482..7697)
                     /locus_tag="IV328_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937621.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7700..8107)
                     /locus_tag="IV328_001476"
     CDS             complement(7700..8107)
                     /locus_tag="IV328_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP8937622.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8373..9848)
                     /locus_tag="IV328_001477"
     CDS             complement(8373..9848)
                     /locus_tag="IV328_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP8937623.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9838..10686)
                     /locus_tag="IV328_001478"
     CDS             complement(9838..10686)
                     /locus_tag="IV328_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8937624.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10723..11085)
                     /locus_tag="IV328_001479"
     CDS             complement(10723..11085)
                     /locus_tag="IV328_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937625.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11385..12009)
                     /locus_tag="IV328_001480"
                     /pseudo
     CDS             complement(11385..12009)
                     /locus_tag="IV328_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12006..12317)
                     /locus_tag="IV328_001481"
     CDS             complement(12006..12317)
                     /locus_tag="IV328_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937626.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12361..12654)
                     /locus_tag="IV328_001482"
     CDS             complement(12361..12654)
                     /locus_tag="IV328_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937627.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12848..13495
                     /locus_tag="IV328_001483"
     CDS             12848..13495
                     /locus_tag="IV328_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8937628.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13556..14701
                     /locus_tag="IV328_001484"
     CDS             13556..14701
                     /locus_tag="IV328_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8937629.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14782..15087)
                     /locus_tag="IV328_001485"
     CDS             complement(14782..15087)
                     /locus_tag="IV328_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8937630.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15112..15543)
                     /gene="ruvX"
                     /locus_tag="IV328_001486"
     CDS             complement(15112..15543)
                     /gene="ruvX"
                     /locus_tag="IV328_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8937631.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15540..15806)
                     /locus_tag="IV328_001487"
     CDS             complement(15540..15806)
                     /locus_tag="IV328_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8937632.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16061..16786)
                     /locus_tag="IV328_001488"
     CDS             complement(16061..16786)
                     /locus_tag="IV328_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8937633.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17147..17217)
                     /locus_tag="IV328_001489"
     tRNA            complement(17147..17217)
                     /locus_tag="IV328_001489"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17183..17185),aa:Gly,seq:ccc)
     gene            17334..18086
                     /locus_tag="IV328_001490"
     CDS             17334..18086
                     /locus_tag="IV328_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8937634.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18102..18581
                     /locus_tag="IV328_001491"
     CDS             18102..18581
                     /locus_tag="IV328_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8937635.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18832..20412)
                     /locus_tag="IV328_001492"
     CDS             complement(18832..20412)
                     /locus_tag="IV328_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8937636.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20405..21148)
                     /locus_tag="IV328_001493"
     CDS             complement(20405..21148)
                     /locus_tag="IV328_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937637.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21324..22100)
                     /locus_tag="IV328_001494"
     CDS             complement(21324..22100)
                     /locus_tag="IV328_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8937638.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22348..23184)
                     /locus_tag="IV328_001495"
     CDS             complement(22348..23184)
                     /locus_tag="IV328_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8937639.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23195..24124)
                     /locus_tag="IV328_001496"
     CDS             complement(23195..24124)
                     /locus_tag="IV328_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8937640.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24316..24669
                     /locus_tag="IV328_001497"
     CDS             24316..24669
                     /locus_tag="IV328_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8937641.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24743..25399)
                     /locus_tag="IV328_001498"
     CDS             complement(24743..25399)
                     /locus_tag="IV328_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8937642.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25625..27559
                     /locus_tag="IV328_001499"
     CDS             25625..27559
                     /locus_tag="IV328_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8937643.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27645..28868)
                     /locus_tag="IV328_001500"
     CDS             complement(27645..28868)
                     /locus_tag="IV328_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8937644.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(28862..30760)
                     /locus_tag="IV328_001501"
     CDS             complement(28862..30760)
                     /locus_tag="IV328_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8937645.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQISEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30754..>30934)
                     /locus_tag="IV328_001502"
     CDS             complement(30754..>30934)
                     /locus_tag="IV328_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8937646.1"
                     /translation="ILQKTEKHYANWNMDEDGIIGGGAEAYHRELTYNVPLIYSDYFF
                     IEGLLRLEQSELAIW"
BASE COUNT         9256 a         6266 c         5060 g        10352 t
ORIGIN      
        1 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
       61 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
      121 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
      181 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
      241 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
      301 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
      361 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
      421 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
      481 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
      541 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
      601 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
      661 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
      721 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
      781 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
      841 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
      901 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
      961 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
     1021 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
     1081 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
     1141 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
     1201 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
     1261 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
     1321 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
     1381 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
     1441 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
     1501 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
     1561 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
     1621 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
     1681 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
     1741 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
     1801 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
     1861 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
     1921 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
     1981 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
     2041 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
     2101 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
     2161 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
     2221 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
     2281 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
     2341 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
     2401 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
     2461 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
     2521 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
     2581 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
     2641 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
     2701 tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca taagcgggaa
     2761 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
     2821 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
     2881 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
     2941 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
     3001 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
     3061 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
     3121 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
     3181 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
     3241 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
     3301 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
     3361 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
     3421 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
     3481 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
     3541 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
     3601 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
     3661 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
     3721 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
     3781 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
     3841 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
     3901 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
     3961 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
     4021 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
     4081 accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa catattgacc
     4141 gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc gtttagtaac
     4201 ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat cggacttgaa
     4261 gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct taatgcacga
     4321 acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg ttgtaggaaa
     4381 ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc tcccttctgt
     4441 tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa caagccatag
     4501 tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag aagtaaaaga
     4561 acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat tgtctgcttt
     4621 cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga ttaatgcagc
     4681 tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt tgttatccgt
     4741 ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt gaataacgtt
     4801 gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa tcgttccttg
     4861 tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg aattagcgtt
     4921 atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca aatcttgaat
     4981 aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa tggaacactc
     5041 accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt cttgaagccc
     5101 atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat ctagcggtac
     5161 gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga tataaacggg
     5221 cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag caatcatata
     5281 gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag aattaaggtt
     5341 attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta cttttttgat
     5401 ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt gaatttcttc
     5461 gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt cgccttttag
     5521 cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa gcccagcggt
     5581 actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa tgacgttgta
     5641 cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc
     5701 atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg gcaagtgctg
     5761 aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact tgaccacgtt
     5821 tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag cgttacttac
     5881 aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat tggcagccat
     5941 gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg ctcgattgaa
     6001 tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa tgaatttttg
     6061 aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa tgaatttaat
     6121 cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact tagaacgctc
     6181 caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa tcagcttacc
     6241 tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa cgggtcttcg
     6301 ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa ggtcatacct
     6361 agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg aaccgctgga
     6421 ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct tatctcggct
     6481 tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc ataatctgct
     6541 tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc acttctagcg
     6601 ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt gaacaattct
     6661 tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt tgtatcgtgt
     6721 aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca
     6781 attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat ttatctatat
     6841 aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt aactcaatgg
     6901 tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa tctcctactt
     6961 tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa ttacccttat
     7021 gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca
     7081 cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc attggctgtc
     7141 ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa ccgtctattc
     7201 caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat caagccttct
     7261 tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc ctttcgccat
     7321 gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc ttcacgctta
     7381 attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc aatcaatgtt
     7441 ttacatccag catgattaca ttgtttttta actgccatag attaccaacc aaagcgatct
     7501 cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg cgaaactatc
     7561 ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc aaaactaata
     7621 aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta
     7681 atactttcca tatgcataat tattctacct ccaataaatt agttatcaat tcatcatctg
     7741 ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc tcattcgatg
     7801 taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat tcatccatta
     7861 gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact agcgattgac
     7921 accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt aatacttctg
     7981 gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc gtgttcatta
     8041 gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt gaaataatat
     8101 tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta acaggtaccc
     8161 gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag gtaacgggta
     8221 aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt ttaaggaaat
     8281 aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt aaactagtaa
     8341 cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc agctcgattt
     8401 ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct ttttggcagt
     8461 tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt agtttctgca
     8521 tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc caaaatatca
     8581 gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc attatagaaa
     8641 tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata agaacgtctc
     8701 gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata tttagtgatt
     8761 gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc aatttctaag
     8821 acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa aagcaccttt
     8881 gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt catctttggc
     8941 ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt caaatactgc
     9001 ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac attccgacca
     9061 taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg ttcaatttta
     9121 gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg aagctgcata
     9181 taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa
     9241 agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac tggaacgatc
     9301 tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca acgttttgtt
     9361 acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag tgtttccagt
     9421 ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt gaaaacattc
     9481 tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac tacattgtag
     9541 ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc tccagcggta
     9601 cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc gttataagca
     9661 attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac aacactgtta
     9721 cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt caattcaatg
     9781 atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa tttatgctta
     9841 tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa tttgattcac
     9901 ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca attctgctaa
     9961 agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta aatgatcgtt
    10021 tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg tccctgtttt
    10081 tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta ggttcactag
    10141 ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt catatgctct
    10201 cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag tgataatatc
    10261 aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa atgcacctgt
    10321 ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc ctttacctgg
    10381 tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt ttttataatt
    10441 ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca tttgatgatc
    10501 ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat taatttgatt
    10561 agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt tagttccagg
    10621 agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt gataattatt
    10681 tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag acacctccat
    10741 gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag caccttttaa
    10801 ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt gcatatcagt
    10861 aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc gacaaattat
    10921 tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat tgaatgaatt
    10981 tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat taatttcctc
    11041 taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact tatgccctcc
    11101 agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg ctaatcctgc
    11161 aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga ttaaaatgct
    11221 tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat ggtataatcg
    11281 gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc gccaagactg
    11341 ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt tgtctgcaat
    11401 tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata tttttcttta
    11461 acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa catttcagga
    11521 gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac agctttattg
    11581 gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc gtggcttaat
    11641 ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg aaagccttca
    11701 agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg
    11761 gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt tgatgatgtt
    11821 tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc catctttagg
    11881 caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa catctttcgc
    11941 cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt tccaaatttc
    12001 agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc
    12061 tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact
    12121 aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag caatttttga
    12181 tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc ttccaagttt
    12241 gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc gattgcttta
    12301 ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca cggcttgcgt
    12361 ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc ttacgactcc
    12421 atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag tattgtatga
    12481 aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact ggtacttttt
    12541 ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc
    12601 aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt ccatatttat
    12661 cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga
    12721 atacatttct atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat
    12781 tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag
    12841 aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac
    12901 aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg
    12961 taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct
    13021 tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct
    13081 attaaacgga tcttatacat taagagatat gcatatgatg cccgaaatta agcgaaatgt
    13141 gcttgaaaag caattgaaaa aaactattga aaaaaataac tctataattg attctaatct
    13201 ttctaatgct atcaataaca tcgaagaaga taacctgact gtctcacaga agatttattt
    13261 aacaaacgca ataaaatatc ttaaaacagg atctcgaatt gaatttcctg acgggagtac
    13321 cccaattgct tatctcgcag ctcttctaaa tacgctttta cgatatgact cattaattaa
    13381 tgattcagaa aaaaatacag atgagaaaaa agagtatcaa gaattttatg aaaatgatct
    13441 tctaaaagag ttcacagctc ttgttactga gctaaataaa cactattcaa aataataata
    13501 aactgcctcc tccaaccgcc cccacggctt gctacactat ggagggaaaa ctattatggc
    13561 aacattcaag caatatgaaa ctaaaaaagg taaattttgg ctctatgaag cctatcttgg
    13621 aatgaacaaa atgactggca aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa
    13681 agaagcccag cttgccttgt cacgcttgca agtggactac gataaaaacg gtattaaaaa
    13741 agcgaacaac gaaacgttta aagaagttta tgacttgtgg ctagaaacat ataaaaccac
    13801 tgtccgagaa gccaccttca tgaaaactga aatcaagttt agaaagtgga ttcttccaaa
    13861 atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag
    13921 ctgggctgaa actactgacc aatacaaggt acttcattca acttctaagc gtatttttag
    13981 ctacgctatc aatcttggga ttattgagac taatccacta gacaacgtat taatgcctaa
    14041 aagaaccacc actaagcata aagaaaaggt caaagtttac agtaaagaac aactgatcac
    14101 cttgtttgat tacctaaaca gcaaacctga aacttatcga aacgactttg ataaaacgtt
    14161 actacgcttt ctcttttata gcggggttag gatcagtgaa gcccttgcat tgaattggtc
    14221 agacattgat tttcaagaaa atacggtcac catcaataaa acactaagcc aatccaaaaa
    14281 tggctataaa atcagtgatc ctaaaactga ttgtagtgct ggggttcttc cactagatga
    14341 aagaacaatt tctatattaa aaaaatggca gattaatcag cggaaatata tgctgacact
    14401 tggtgtaact gatccgacta tgattttttg tggtatctat aaagaactaa tcacacacca
    14461 cgctatttat gcaagattga ttactatttg tgaaaaagct atggttccat ttctcggtgt
    14521 gcatgtagca agacacactc atgcttcaat gctcttggac gctggagcta ctatggtaga
    14581 agttcaaact agattacggc atacaaaaat tagtcagacg atcgattgtt acggtcactt
    14641 ggcaaaagaa acgaaagaaa agactgttga aaaattagta caacacttaa atattgacta
    14701 gatggcatac aatatggcat acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa
    14761 ggtttctcct agctttatag tctattcttc ttcttccgcc atgaatgtat tgaatacttc
    14821 ttcaatcatg tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc
    14881 ttcgttttcg atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc
    14941 aggatataaa agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagtaggat
    15001 ttcatataat gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcatg
    15061 gtcgtgggaa tggttatgtt ctgtcataag attacctctt ttcttttatt attaaaataa
    15121 ttgcttacct gagccgtcta aataattttg taagatcatc acagctgcta atttatcgat
    15181 gacttttttt cgtttggctc ttgaagtatt tgcctgctct actagcatac gttctgcctg
    15241 aacagtggtt aatcgttcat cttgataatc aactggcaag gagaatagtt ctttgatctt
    15301 ttctccgtat gacatggaag cttctgccct tggtccgatg gaattgttca tatttttagg
    15361 taatccaact acaaaccggt ctacttcata ttgtgctact aattccttca accgatcaaa
    15421 gccaaactct tcttctgctt cattgatccg aatgatctct actccttgag ctgtccagcc
    15481 aaaggggtca ctgatagcaa cacctactgt cttagaaccg acatccaagc ccattactct
    15541 cataaattca ctccatgatt tgagaggtag tccttgacaa ttacttccat gatctcatcg
    15601 cgttcgtgac ggcgaatcaa atttcgagca tcacgataac gaggaatgta ggccgggtct
    15661 ccagaaagca agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc
    15721 cgataaacaa tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa
    15781 cgtacggttt catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag
    15841 cttaccttct acaaccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa
    15901 acttccgaat aaattaagaa acactatttg tatgtttttt tccttttttc tgtagcttta
    15961 tcaaaagtac tcttttcata aagtatacgc tatatttctt tgaatgtatg tttttacaca
    16021 attttttcat aatattcata aaaaattcga atagctgcta ctaatttttt gtttttgttg
    16081 cttgtcctgt ctcataattg atcgtgtagc catcttgaac attgtagata aagacattga
    16141 attcaatttt tttgtcttca atgctttctg cttccaattg cactcccctg gctaccagtt
    16201 cattcccctc aaaatgagga gtgacacggt agcgtacatg atgatttgtt tctttgatat
    16261 atgccgcaat atcattttcg tgagcaagca tgtatggagc attcaacgaa cgtgtccctg
    16321 tcatcaagtt tttaatgttg ttattttgac cagtcagctg gaaaccaatt aaatggctgc
    16381 gattatataa ccattgcccg tcacttaatt tcttttgttt ccaacctgtc gggtcaatat
    16441 acaaaggctc tctcttttca gtagggaaca aatcttttcc aagcatagca ttggcttgtc
    16501 ctactctgtt caatttatca atatttgaat agctttccca tgttcctttg gaaaggctaa
    16561 gatcctcttt tgtaaattcc ggctgattcc cattgatttc tatttcttga tgttttccat
    16621 catattctgg cacatccgct acttgagaag cttgtggtgt ggaatcaaaa atagttgtaa
    16681 tctgctgaaa gatatctgaa tctttttgtg tggtggaaac accgaatgcc cctgcaatca
    16741 cagcagcgac gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt
    16801 actaacagct ccctaaatct taacatatgt tcccctataa ataaatacta gaaagaaata
    16861 gaaacagcaa aaaataaaac ctgttttaag atggcttaaa aatacaaaaa agagacggat
    16921 tcctcgtctc taatcagtca ccactctatc tgtaaggtat ttattattat tcaaaaattt
    16981 tcttcatact caagacaata cactgatttc acaaccgctt ggtaaacggt gcgcaaaagc
    17041 ttgcatctgc ggtaataagt ccatccaagt taaaaacata agaagttgtt tttgcttggc
    17101 tgttcttatt acttgttgca gagcagcttt ttcacaacct attttaagcg ggtgacggga
    17161 atcgaacccg cgacatcagc ttgggaagct gggattttac cactaaacta cacccgcaat
    17221 acaaaaacag tatacgcctc ttttattcct tcctcaagtt tttttattct ttacgcttct
    17281 ggtatgtttt gtagtaaaat aaaactaatc aatcaattat tggagaagat gctatgacgg
    17341 gaaaagaatt agatctgttg ctggacactt gtttactcgc tgggaaaatc atgatggaaa
    17401 gcaatgcgga gatgtatcgg gtagaagata cgatgagtcg aattgcttta gcttctggaa
    17461 atttccgttt ggtcagctat gtcactcaaa ccggtctctt tattggtctt gatcgtacct
    17521 cgaccatccg aatggaacaa attaccaacc gttcgattaa tttagaaaaa gtagtaaaag
    17581 taaatgatct ttcacgtaaa tatgttgctt cagagctaac attagaagaa ctttactgcg
    17641 aactgcaagc aatcgaccga gaccgttctt tctttcctgt ctggttgcaa atcgttagcg
    17701 cggcagtgat cagtggaaca attatggtgt tatttggcgg aaagatgacc gatttacctg
    17761 tgactttagt cattggagga ttgggatacg cagcttatct atatagtttg aaatttttta
    17821 gaatcaaatt tttatcggaa tttttatctt ctttattgat tggcatagcc gctatctaca
    17881 gcgtacgttt tggcttagga agcaatcagg atctaatcat tatcggatgt gtgatgcctt
    17941 tagttcctgg agttcaaatt acgaacgcta tccgtgattt gctagctgga cattatgtct
    18001 caggagtttc tcgcggaaca gaagcgatga tgactgctac gatgatcggt tttgcgattg
    18061 cttttatttt ccagcttttt tattaggaaa ggaacacaag tatggggacg atatttattc
    18121 agttttcttt tagtttttta gcgactgcgg catttgccgt tattacaaat gtacctaggc
    18181 gttcattgat ttgttgtggt ctttctggtg cttttggctg gatgatctac tggatctgtg
    18241 ttcaaatagg cggatctgct gcttttggtt cattgcttgg ttctttagga gtagcattca
    18301 tcagcgatct tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat attcccggga
    18361 tggttcctct agtccctggt ggtttagctt atcaagcggt tcgaaatctc gttactgaat
    18421 cctatcgaga agcggctcat tatgcggtcc aagcaattat gattgctgga gcaatcgctt
    18481 taggtcttgt attatcagaa gtctttaacc ataacatccg taatttcaaa gtaaagcgtg
    18541 acgcaatcat gatgaaacga aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa
    18601 aggttgtgaa agagacgttc agccttgagc gatgacaaaa tatctcacaa acattgcgac
    18661 aagtaaccgc aggagcaata agaagtaatt atgagtatta aaaaaagttt tgtaaaaata
    18721 gcttctcaca tttttacata aaattgactt aatactgaga aactgacttt tgaacaccct
    18781 ctatctgtaa aaaaggggct gcgacatgat ttttgtcacg gcccctcttc tttattcaaa
    18841 ttttttccaa aacgttcgtt gataataaac gatccataga gcggaaagta ttagaactaa
    18901 accgatcaca ggaatattca ctaaccaaaa tggataaaac aaagccaaca ttccgtataa
    18961 gacgataaga ataatactga tgatcaatga acccatcata gttgcgacta aacccaaact
    19021 tcctgaacct ctgttaaata attggttaat attggtccag ttcagcagca gcaaccgata
    19081 atctctagcg aaataacgta gtgacaataa gtagcagccg aataaagaac caaatagcaa
    19141 gctagggata aaaaacaaag gcagctgaaa agacagtccg cttaataatg cgatcccacc
    19201 agttaaaaaa ctctgcaata tccatccgaa tctgaacttt tcttttaaat attggttcat
    19261 agagattggc aaagaacgga taaacagaaa attttcttga tccaaagaaa taagattgct
    19321 gataaaagaa gtctgattaa ctgttaagaa agccaatgcg atccctacca ggaaaacaac
    19381 accgattaac ttattatcta aatcagttaa ttttatttcg cctcctaggg caaaagcaag
    19441 aataaaaatg attggagtta acaaggaaga agaaagaact tgcataatca aattaggttc
    19501 ttttactaat tgagcattgt aactaaataa aagctgatgc acattttggt tctttttatg
    19561 ggttcttttt attttgaact cactgtttga atcagtcagt tgctctgtaa gctttggtac
    19621 tatcagctgt tttacagcaa ggaaaaatcc cagagccgtg atagctagta ttccccaacc
    19681 caataaacct tgttgagaaa gtggctgact ggcagcatca tagaatggca ataatatcga
    19741 aatcggatgc cgatctgtta actcattttc aatagtcccc gtttgatgat tcatccacat
    19801 gattccagca aatgcgatca gcatcgagct gccaagcaag atactagaaa ctgtttttct
    19861 atgtttttta aacactgctg ttcgagccaa accaaaaaca atcaaactag ccagacaaaa
    19921 aagaataatc aaaaatagag aaaacaaaag gattgagata atgatagtaa gcaaaatccc
    19981 tagatcagct cgccatccgg ttaaaataaa catgactaga agcggaaaaa caaaaggaat
    20041 gatggtcaaa gcaactacca aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag
    20101 aggcaaaaat acttgcaggt catttccttc aaaaaagata ttataaatga ctgatatact
    20161 ttgagttaaa cctaacatac caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt
    20221 aacaaagttc atagcaaaca tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa
    20281 aagattttga gacaaaagat agatagttaa tcgctttccc ttttttcctt tctctcttgc
    20341 tttattcgtg agctgtggat taacatataa aagattaact cgaattaatt ctaataattg
    20401 tcgcttatac atacggtcca tctcctgtcg cttgccttcc agccattttc aaataaatct
    20461 tttctagcgt ttcatcaggt gagccattca ataattcact aactgccccc tgataaatca
    20521 attcaccttt cttcaaaata gccagtttat cgcataactg ttgtgcggta tccaatacat
    20581 gtgtagaaaa aatgactgtt tttccttttg cggcatgagc cttcatcatt tctttcaaat
    20641 caaatgctgc ttgaggatca agtccttgca aaggttcatc caatacccaa atatctggat
    20701 ctggcagcaa cgtgccgatc aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga
    20761 ttgtttcatt ttgatgacta tacatatcaa aaagcgctgt caattctgct aaacgctttt
    20821 cttttttttg tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat tctccagccg
    20881 tcaattgtaa aaaaatatct ggggaatcag gcacataacc gattttttgt ttgatactca
    20941 atcgattttc cgtcaatttc attccatcta ctaaaatcgt tcccgatgat ggttcaataa
    21001 tactaactaa acttttaatc gtagttgatt ttccggcacc attatgtcct aaaaatccaa
    21061 aaatttcccc ttgtcttatc gagagagaca agtcttttaa tgcttgtttt ttcccgtaac
    21121 ttttacttac attgatgaat tcaatcattt ctgtactcct tttgtttgtt attacttttt
    21181 attgtaccct tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata
    21241 gaaacgacta ggctgctact tgggttgttc tcttctacag gaaaacactc ttctaacagg
    21301 taagatatga gtctttgttt tctttaaaat gattcatttt ctatcttttc ttgcataaaa
    21361 ttctttagtt tatcaatact tctctttccc atctctagtt gttctccatt tgcaaatgtt
    21421 actatttcgt ttactctgct aatagatgta atgttttcag tattaatgat ataagctttt
    21481 aaatctttac ataaatactc atattcgagt gattttttat attttttaat tgtgccgttt
    21541 acgagaatct cttcatcttt caatactagg cctattttat ttttataacc tggcatgctc
    21601 attagataaa ggatttcatc gtaatggata tagtgatcgc tattaccata acggacagtt
    21661 gtccaccttt ttttcgtagc atgttgaaca ggatagtttt ctttttcttt caataagtaa
    21721 ctttggatgc tttttttatt tttttcataa gtgaattctt ttatcaaata accactaggc
    21781 gtaatctttc gattaataac agttattgct ttattcggat gagacgttag ataaacaatt
    21841 ttacactttt cattcttttc tctaagcatt tcgcctagtt cgataccgct ataataggcg
    21901 ttcaaatcga tgtctaggaa aaaaatatct gaggaataga tttcattatt tttaatgaat
    21961 tcaacaaatt gtataacgtt caatccctga acgatctcaa atttccaagg acttaataaa
    22021 attctggatt cttccaaaat ttctactagc attttttcaa aaaccaaact atcatccaaa
    22081 ataaatatac gcgttaccat aaaaagtacc tccactcgat attttactat caaaatcatt
    22141 tcatacaact tttcaacata catgttagga ataaattgta attctcttca caatcttttc
    22201 caacaccttt gaaaccgata tcaaatactt tcaggatgct gcttctaaaa taaatatcaa
    22261 acaaatgaat acatcatgca aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc
    22321 tgagctatgt ttatttattt tgttctttta aaaagaacga tacacatact gatttttcgg
    22381 agcagagact ttctcagcgt ttgtaacaac tacagatggg atttcacgct tgaatggttg
    22441 ataatagtct gattggatgg ttccttctat tttataccat tcatcatttt taaccgttac
    22501 tccttccggc atatgcgtca acaaaccaaa gactcctgaa tctgccacac aatggataat
    22561 gccgaatcgg aacaagaaga tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc
    22621 ttcatagctg atttttttgc caatgaattc gctagggtaa ttataaatta gctccataac
    22681 ttctaaatag ttttcatctg tgatcttgat agtttgctta tccccatact tttctttcaa
    22741 ctcattcatt tggtctaagt agtctgattt gttgaaataa atactggtat ccggctttaa
    22801 gtattgtgtc tgcatctccg gatcccctac tgattcttta cttactggga aattaaatcc
    22861 tttggcttct acgatcgtag tgtcgagact aactgttgga aacaacgtcc ccacgattac
    22921 tggcaaagct agcagtacat aagcgattcc acgttgataa ggttttgaca acccatggtc
    22981 atgatcgtgc tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca aatacagctg
    23041 aactaaagcc aaaagagcag ataaaatcat tgaaagaata gctaagtagc gataatggac
    23101 attgatatat tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt cgaaataccc
    23161 agataaaatc aaaaaccgca gcatattttc cctcctaaat aaataatgtg aatccaataa
    23221 cgctaagggc aatcagtccg atcagcgata aaataaagcg tcctgtaaaa tatcgtttca
    23281 tcatcagtag atttttgata tcgaccattg ggccaaagac caaaaaggca gctacaggtg
    23341 ctgtgccgaa cagacttaac aaagaagatc ctataaacgc atctgcttct gagcaaagag
    23401 acatcgtaaa ggccaataac agcatgatga taatggctaa aatctttgtt gagccgagcg
    23461 ttagcataat tctagttggt aaataagtct gcatcgcagc tgcgattaat ccgccaatga
    23521 tcaaataacg acctgtatca aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt
    23581 gatggatcag ctgatttttt ggttcattct tctcactttg ttcttcttca tggacatgat
    23641 gatggtgatt ttctgctact ttagacgtga ttttttcttt caatacagcg tctttttgaa
    23701 aataggcaag ccagacacca atcacaaggg caacaaaaaa gctgcctatc acccgccagc
    23761 ttgccatctg ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg attggattga
    23821 tgatcggagc agtcagcata aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa
    23881 attgatgaac gatcggtacg atgccgcatt cacatgaagg aaagaaaaat cctaagatcg
    23941 aaccagtaaa aatagagaag atacgatttt caggaagcca tctcttcaca cgttctgggg
    24001 tcaaaaatac ttgtaaggcg cctgagataa gacagcctaa taatacgaaa ggtaatgctt
    24061 caataacgat cgataagaaa atcgtactca tctgcaaaac cgaatttgga ataaaagaaa
    24121 acatgagttt cctccttttt atttctagtt cattcgcttt gcaaatatac gcttttattc
    24181 aaaaaaaaca agttgctctg taaattttta cacaactctt attttttacc aaagcacttc
    24241 aattgacttt ttctgttatt cccattaaat taaggtctca tacatatcac ttctatcata
    24301 aaagggagaa taaaaatgaa acaaaccact aacttgactg aagttcaaga catactgcaa
    24361 cagtcatccg ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt
    24421 cgtccacgac tagaggaact tctcacacat tatgatattc cggcacttca tttggatgca
    24481 tttgaaacac cagaagtagc tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt
    24541 tttcatcaag gaaaagaagt ctttcggcaa gcacggttca tcgactttaa aaaaatccgt
    24601 tatctactag atgaactgac agcagaagat gattcccttt cttatgaaga aatcttccga
    24661 acaaaataat cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt tttgccttca
    24721 tttgttcaat cgctttttat ttttattctg ctgtattttc ggaatgcgca tttgcgtcag
    24781 aatgcaagaa atagatcaag gtttgcaatt cattcgtcaa atcaacattt tggatcaata
    24841 catctgacgg agcattcaat cgagcagcgg taaagttcaa gattcctttg attcctgctt
    24901 ccgttaactg attgactact tcttgtgctc tttcagcggg tatcgttaaa attgctactt
    24961 cgatttgttg gatcttgatt tgttctgaca tatcttccat tggataaacg gggataccgt
    25021 ctacgatgcg cccaacgatt tcctcattca catcaaaagc acaactgata cgtatgctat
    25081 tactttgatg gaatttgtat tttagtaaag cacttcccaa gttccctaca ccgatcagcg
    25141 caacatttgt caattgatcg tcgtttaatg tctttgcaaa aaagtgcata agatcttcta
    25201 catcataacc atacccacgt tttcctaatt cgccaaaata agaaaaatct cgacggatcg
    25261 ttgcactatc tacttgaacg gcttcactta attcagttga agacactttt ttctttccag
    25321 cattatttaa aatccttaaa tagcgataat acaaaggcag tcttttagct gtcgcctttg
    25381 gaataatatg atctttcacg aaatgacctc cacgtattgt ctaattacat ctttgatttc
    25441 tccatcatag cagtatttat aggaaaaaat caattccatt gctcagatat tgtgaaattt
    25501 tattctttat tcatttattt atcatttctg caaatctcgc agtataatgg aatttatggt
    25561 agactactaa ttgaaaaata gttttttatt tgaggagaat tcacaaagta gaaaagagga
    25621 ttccatgata ttattacaag ccaatcagat tgctcgccat tttggtgcag aagtcttatt
    25681 tgaaaacata catttagaaa tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg
    25741 cgctggaaaa tcgactttat taaagatcat tgctggaatt gaagcacctg atgaaggaaa
    25801 gatcgctaaa aataaaacag cgactctcgg atacctcgct caggacacag gacttgattc
    25861 caatgaaaca gtttggaacg agatgctgaa agcttttgaa caagttagac aaatggaaga
    25921 gcggatgcgc gagctagaga ttttcattag cgaaggtacg ccagacacac ctgcttatag
    25981 ttcttattta aaagaatacg accggctgca acacgatttt tcagatcgaa acggctatgg
    26041 ttatgaaaat gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt cattttatga
    26101 tcagaaaatc gatactttat caggcggaca gcgcacacgt ttagctttag ctcgcatgct
    26161 tttgcaacac cctgacattt tgattctaga tgaaccaacg aaccatttag atatcgagac
    26221 actttcctgg cttgagaatt atctgcaaag ctatactggt gcattattga ttgtttccca
    26281 tgaccgttat tttttagata aagtagtcaa tgaagtctat gaattaagca gaagaaaaat
    26341 gcgccattac aaaggtaact attcgaaata tctagatttg aaagcagaac agttagcaag
    26401 tgaatggaag gcttatgaaa agcagcaaac agaaatccat aaattagaag acttcgttgc
    26461 tcggaacctt gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa agacattaga
    26521 aaaaatggac cggctggatc gtcctcaagg ggatgaaaaa caagcaaact ttctattcag
    26581 tatagaaaag ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta tcggatatga
    26641 taatgtcacc ttatctgaac caatcaatct agatatccgt cgtcaagaag cgattgctct
    26701 agtaggtccg aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc accagatccc
    26761 ttttatccgt ggaaaagaaa cgctagggac aaatgtcacc atcggctatt acgatcaagg
    26821 tcaagctgat ctgcatacaa acaagacgat cctagcagaa ttgtgggatg agcatcctac
    26881 cactccagaa aaagagatcc gaagtgcttt aggtgctttc ttattttctg gcgaagatgt
    26941 agaaaaacca atccctctgc taagtggcgg tgaaaaagca cgggttgctt tagccaagtt
    27001 agcgatgaac aaagaaaatt ttttgatttt ggatgaacca acaaaccatt tagatattga
    27061 caacaaagaa gtattagaaa atgcattgat cgattatgac ggcacactgc tttttgtttc
    27121 ccatgaccga tatttcatca atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg
    27181 cagcaaagtc tatctaggag actatgatta ttatttagag aaaaagcagg aagaagaaga
    27241 aatcgccgaa ttgttggcac aagatgagcc agaatctgct cctgctccca cgaacaaatc
    27301 tgattattac caaagcaaag aacagcaaaa gcttatccgt agccttcaac gaaaaattac
    27361 tcaggttgaa gaagaaatgg cgcggatcga tgaatggatc gatcagttgg aacaagaaat
    27421 ggtcgcgcca gaaaacctgg aagatcacgt aaaactcaat gagctgaacc aagatttaga
    27481 agctgctaga caagatcaag aaacaaaatt gacggaatgg gaagaactaa gcttggaatt
    27541 agaagaatat gaaaattaac gaaaaaacca aaacagaaac cattcatgct gttttggttt
    27601 ttcttttttt attttatcaa acaaaaattc catatttacc tttttcacca ataagaagac
    27661 caattactag tcattctcac taaagcttct aaataatagt aatcacccca aatacaaggt
    27721 gtatttaccc cattattttt gggcttatta taaacagact gtgttaaaaa gccatcttct
    27781 tcttccgttg tagcatatct ttctttcaat tgtcgcataa ttttccaaac agcagactgg
    27841 tataagttct tttctgtttt gtctaataga cctgattcta ttaattctaa taatccacaa
    27901 accatgatca ccatactaga tgtatctttt tcttctctgc tagaatcatc aaagatcaaa
    27961 tcccaataac aaatggtatc ttctggcaac cgattcataa aataattggc taatattttg
    28021 cttaaatcaa aatatttttg ttctttcaaa taacgataac ttaatgcaaa accgtaaatc
    28081 ccccatgctt ggcctctagc ccaacaggaa ttatcactat aaccttgatg cgtacttcca
    28141 tatcttggtt ttccatcttt gacatccata taaaatgtat gataagttga accatcttct
    28201 cttactaaat aatctgctgc attttgaata tgagcctgag cagctttttt atatttttga
    28261 tttccagttt cttcactcgc ccaatacaat aatggaagat tcatgcaaca atccatgatc
    28321 attctacctt gttgtgaagg atcactagga tctccccaag cttgtataac tcctgccttc
    28381 tcccaaaaac gctgaagtaa tgtttctgca gcatcaattg aagcttcctt catttttcga
    28441 tttcccgtta ttttataatc tgctacggtt gataaagaat acaaaaaacc aagatcgtga
    28501 tgattagtga taatttgaca gtcaatcctg tggataaaat ctatatctgc tttttcagct
    28561 gtttgttgaa ataaaggatc acccgtcttc tcaaataaca accaattaat tcctgtccaa
    28621 aatccttctg tccaaccaat attgtctctt aatggataac gtccttttat aggacactcg
    28681 cttgggaatt gatactgact gttactgata cgtttgatta acctctccgt gtgtctgcga
    28741 caacgctcga tctcattatt ccattctttt tccattaatt ctttataaca ttcatttatc
    28801 catttttctg gatgtattat ctcttcaaac atcactttat caagcatttc cccaccagcc
    28861 tttagcataa ttttagtctt tttttattgt ttttattttg gatagttagc ccggttgtcc
    28921 catcaaaatc aattttggga cgattgttcc atacttctaa actataatcc cgatcttgat
    28981 gagcaaccac tcctgttccg agccaatggg ttccttttcc aaatgtaccc tctaatccag
    29041 gaataaaagt gttttcccat gtcatcaagt ttgtattggg caaacccata atagctgtgg
    29101 attttcgttt aatagtatcg tcacttaaac agataatacc tgaaaagcca gcttcactca
    29161 ccctatagtt ttccttgtct tcttctatga ttattccttc tttctcagga gaacattcaa
    29221 tagcaaaccc tgtctcatat agaacatatt ctgtatctaa ttctactttg tgtacgcgaa
    29281 tctgccagcc ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa gtaggttccc
    29341 atgtagaggc aagtacctct ttcttacata caatgtctcg tacattattt cgaacgatga
    29401 actgtccata ggttttgatg ctctcattct taaaactggt cgtgatatat gtgtccgctc
    29461 gctgaatccc tagcattgaa tccattgcca tttctgcttt caattgattc gctcgcggta
    29521 tcgaaaaacc aaattgacta gaataagcaa attttaaata tttttcattg gtcaatgtat
    29581 ggaaatttgg ccctaattgt cccgcattca aaaaaaatgt atgtccatta tcatgttgta
    29641 tgagcaaatt ggcttctgaa agaagtttag ttccttcacg aataggcata ggcatttctt
    29701 tagccgtcca atactcatgg tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc
    29761 ataatggaga taacgtacta ttatagggtt cagtcaatgt caattgaggg taggtataac
    29821 caatattcag gatacctttt tcatcaaata tttctttttg catccaccat cttatattcc
    29881 gattgactaa ccccttgatt tcacctaatt taaaaggcaa aagatcggcc cacaacatag
    29941 ctgaccaaaa gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg
    30001 caccatcttc cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct cggttcttat
    30061 atatctggct aatgggcttt tctggttcta gtacgctata aatcaaacca taataatgaa
    30121 atgcaaaagg attataataa tcttggcgac ctcttgaact gtctcgatac cagccatttt
    30181 gaagataaca attttctata ttatcaaatg cttctttcag tgctgattct ttaactggat
    30241 aaccaaattt ttggataatt gttccgacaa ttatcttaaa aaattgccaa ttaccatctg
    30301 cacattcata ttcagaaatt tgcgagaacc aatgaataat ctggcttttt tctcctgtat
    30361 ctaatttttt ccataataat tgttcatgat gatataacgc taataacatt gcgggcattt
    30421 ctaccatacg ctggtctctt tcatgtattt ctccccaata atcgggatgg gaaggatctg
    30481 ttccagcttt tattcctgat agaattaatt tcaaccatcg ttcatctaat tggtcaattg
    30541 cgggccccgc tccccataaa acccgagaga atccttccat tcctgcaatt ttatttccat
    30601 atcctacccc ttgatgtata taatcaattt gtgcgccact tgctcgataa aacggctgga
    30661 gtggttgatt tatcttttta aataagtcaa agtaatcctg tctggtcctt aatagtggta
    30721 ctggatgttt cattgtattt tctcctcttt ttactaccaa atagctaatt ccgactgttc
    30781 taatcgaagt aagccttcaa taaaaaaata atcagaatag atcagtggca cattataagt
    30841 cagttctcta tggtatgctt ctgctccgcc tccaatgatc ccatcttcat ccatattcca
    30901 atttgcatag tgcttttctg tcttttggag tata
//
LOCUS       DACDKJ010000030        30792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDKJ010000030 DACDKJ010000000
VERSION     DACDKJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.030"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..799
                     /locus_tag="IV328_001503"
     CDS             <1..799
                     /locus_tag="IV328_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8937647.1"
                     /translation="YQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAA
                     FPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQF
                     SNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPK
                     NLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSM
                     DFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            948..1286
                     /locus_tag="IV328_001504"
     CDS             948..1286
                     /locus_tag="IV328_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937648.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1491..2066)
                     /locus_tag="IV328_001505"
     CDS             complement(1491..2066)
                     /locus_tag="IV328_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8937649.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2798..3379)
                     /locus_tag="IV328_001506"
     CDS             complement(2798..3379)
                     /locus_tag="IV328_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8937650.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3562..4188)
                     /locus_tag="IV328_001507"
     CDS             complement(3562..4188)
                     /locus_tag="IV328_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8937651.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4210..5541)
                     /locus_tag="IV328_001508"
     CDS             complement(4210..5541)
                     /locus_tag="IV328_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8937652.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5696..6097)
                     /locus_tag="IV328_001509"
     CDS             complement(5696..6097)
                     /locus_tag="IV328_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8937653.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6183..6389)
                     /locus_tag="IV328_001510"
     CDS             complement(6183..6389)
                     /locus_tag="IV328_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937654.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6484..7986)
                     /locus_tag="IV328_001511"
     CDS             complement(6484..7986)
                     /locus_tag="IV328_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8937655.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8004..8459)
                     /gene="argR"
                     /locus_tag="IV328_001512"
     CDS             complement(8004..8459)
                     /gene="argR"
                     /locus_tag="IV328_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8937656.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8557..9174)
                     /locus_tag="IV328_001513"
     CDS             complement(8557..9174)
                     /locus_tag="IV328_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937657.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9395..10768
                     /locus_tag="IV328_001514"
     CDS             9395..10768
                     /locus_tag="IV328_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8937658.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10843..11955
                     /locus_tag="IV328_001515"
     CDS             10843..11955
                     /locus_tag="IV328_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8937659.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(12027..13664)
                     /locus_tag="IV328_001516"
     CDS             complement(12027..13664)
                     /locus_tag="IV328_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8937660.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13832..14440)
                     /locus_tag="IV328_001517"
     CDS             complement(13832..14440)
                     /locus_tag="IV328_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8937661.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14824..15975)
                     /gene="queG"
                     /locus_tag="IV328_001518"
     CDS             complement(14824..15975)
                     /gene="queG"
                     /locus_tag="IV328_001518"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8937662.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15994..16746)
                     /locus_tag="IV328_001519"
     CDS             complement(15994..16746)
                     /locus_tag="IV328_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937663.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16887..17837)
                     /locus_tag="IV328_001520"
     CDS             complement(16887..17837)
                     /locus_tag="IV328_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8937664.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17838..18677)
                     /locus_tag="IV328_001521"
     CDS             complement(17838..18677)
                     /locus_tag="IV328_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8937665.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(18674..19420)
                     /locus_tag="IV328_001522"
     CDS             complement(18674..19420)
                     /locus_tag="IV328_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8937666.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            19658..21028
                     /locus_tag="IV328_001523"
     CDS             19658..21028
                     /locus_tag="IV328_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8937667.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21083..21979)
                     /locus_tag="IV328_001524"
     CDS             complement(21083..21979)
                     /locus_tag="IV328_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8937668.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22260..22937)
                     /locus_tag="IV328_001525"
     CDS             complement(22260..22937)
                     /locus_tag="IV328_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8937669.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23028..23933)
                     /locus_tag="IV328_001526"
     CDS             complement(23028..23933)
                     /locus_tag="IV328_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8937670.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24108..25562
                     /locus_tag="IV328_001527"
     CDS             24108..25562
                     /locus_tag="IV328_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8937671.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            25578..26609
                     /locus_tag="IV328_001528"
     CDS             25578..26609
                     /locus_tag="IV328_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8937672.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            26616..27803
                     /locus_tag="IV328_001529"
     CDS             26616..27803
                     /locus_tag="IV328_001529"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8937673.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27959..29341
                     /locus_tag="IV328_001530"
     CDS             27959..29341
                     /locus_tag="IV328_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8937674.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            29313..29699
                     /locus_tag="IV328_001531"
     CDS             29313..29699
                     /locus_tag="IV328_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937675.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29899..30567)
                     /locus_tag="IV328_001532"
     CDS             complement(29899..30567)
                     /locus_tag="IV328_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8937676.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8678 a         6274 c         5639 g        10201 t
ORIGIN      
        1 ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga cgtccgcttg ttttacccga
       61 ggaacaatca gaatatattc aaagaaaggt tgttcaagga tggacacccg atgtgattgt
      121 tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc atttatcgta tgttcaaaaa
      181 ggggctattt gattcttctg acttaccgat gaaaggcaag cgtaaaccga atggacatca
      241 agaaagacgt ggaaaacaaa ctttccgccg ctctattcat gaacgtgaaa aggattatag
      301 ccaattctca aatgagtttg gtcaccttga aggtgacact atcgtaggtc tgaaacataa
      361 aagtgctgta attaccttag ttgaacgatt atcaaaagtt atcatcacat tgaaaccgtg
      421 tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa tggtttgaat ctgtaccgaa
      481 aaacctattc aaatccatca cttttgattg tggaaaggaa ttttcaaatt ggaaacagat
      541 cagtaatgtc aatgatattg ccatttattt cgctgatcca ggaacgccgt ctcaaagagg
      601 cctaaacgag aattctaacg gattgttacg tagagatggt ttattgaaat ctatggattt
      661 caattcagta gatgaatttt ttattcaatc tgtcgcatct aaacgaaata atattcctag
      721 aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa gtattgatga
      781 tctgtctaac ttaatttgac aattaagaaa ctaaaaaaat aatccaagaa acattgaacc
      841 tatggatcca aaagctaccc aaatactata gctaaccccc cgctaccttc aattttgcag
      901 ataaaacgct ttgggagaaa atttcaaaga caacaggcca tcctatcatg cccgattata
      961 aacaaaaagc tggctattcc ttttgctctg acaaatttca attgggaaag ttcacgaaac
     1021 tagcaggtga agaagtttca actgttcgga tcaaagaatg cccgcttcaa atagaaacat
     1081 tggttcaacg aatttcaact agacaagact ttgctattgt agaacgtgag atcgtagcta
     1141 tttttgtaga agaagggatt ttacatgatc caactcatat caatgtggat aaatggcaac
     1201 ctcttatcta taaattcaga gaatatacaa ctgccaacca gtcgttaact ttgaatttta
     1261 gattccaaga attcaaagag tcataatgat aaacatcgtt aggtataaat aaaaaagact
     1321 cgcagcacga gtaaaaaaca aatagaagct atacattaga aaggtcgact gtgaccctat
     1381 agatgcagta tatgcagctt atgagcattt cttctgttct ctttgttttt gggatctgtg
     1441 ctgaagtctt tttcgttttt tgccgctctt tctttgaaaa atcgattatg ttataccttc
     1501 tccattacta attcaggcat ctgctgagta atgatttttc gcgcaaaaga aatatctgtt
     1561 acccaaggtt cgatttgttc ttcagtcaag acaagcggca ttcggtcatg gattggtgcg
     1621 actgtgcggt taggttcagt agtcattatg attgattctg ccattttttc atcctgatgg
     1681 ctacgataaa aaccgccaat atacactatt ttttccttat ttgaaaacat aaatttgtct
     1741 ttttcttcat tccactcata aaagccactc attgggaaaa cacaacgatt ttgcaaaaat
     1801 ggttgatgaa aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt
     1861 ccttttttaa atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc
     1921 tcttttctta acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct
     1981 gcctgcttcg gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct
     2041 agatcaaaaa aataacgacc acacatccta atccctccca atcttttttt ctatcataca
     2101 ctgcaacaag aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg
     2161 tgacaagtga caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc
     2221 acaatattca acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga
     2281 aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag
     2341 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttaa tactcgaaac
     2401 taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
     2461 taagcccaac caaacgaaaa atatgagcag ctatttttat ttggctgttc ttattacttg
     2521 atgcagggca gctttttcac aacctctcca ctaaacggtg ttcaatcagc taaccttcgg
     2581 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
     2641 caggtcaaag cgctgatttc acaacctcta atacacggtg ttccagaagt agcttcttcg
     2701 aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
     2761 tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta
     2821 acaatctttc tttgatcaat tgaaaattct gcctaagtag tgatagatcc tgtgtgactt
     2881 tcaacaataa gatacttccc tggatttcag caatcaacgc tttggcttga cggtctgctt
     2941 cttcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc
     3001 attgattgac taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa
     3061 taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa
     3121 tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt
     3181 cgaacaattc tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt
     3241 tttttgaaga aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat
     3301 ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga
     3361 tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac
     3421 agtaaattca tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac
     3481 acagccctca ctataaaact gttctcatat agctagatgt tcattgattc aagaactgcg
     3541 aggacaaaag caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac
     3601 ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc attgcttctt ggccaggagc
     3661 accaattgca tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg
     3721 ggaatcgcta cacaagtttc cgttcattcc accaagaatt actgtgtcga ttccattttt
     3781 tctcaattgg agcgccaaat cattgctttc tggtccaaag attttatgag gagagctgat
     3841 gatcgtattt tcatccaaat aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc
     3901 ttccactgat tcatattgac tttttctagc aaacatctgc tgctcaagca tcatatgttc
     3961 tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa
     4021 gagtttgtat cctttttctt ttgcttgttt taccatttca atcatatttt cgatcgtatt
     4081 gtatttttct aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc
     4141 gacttgcggg tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc
     4201 attcgttatt tattttaata catcatttag agcttcactc attgtcggat gagaatagat
     4261 ttgatctctc aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat
     4321 gacttcatga gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc
     4381 gactaaaact ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct
     4441 tgtcatatac ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa
     4501 acctacattg gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt
     4561 cgttgttcgt ttattctcgc ccttcaactg atccataatg attcgataat catctagaga
     4621 gatataagtg aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc
     4681 tgtacgaagg tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt
     4741 ttctaatcca agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc
     4801 ttggattgtt acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc
     4861 tgctacttga tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc
     4921 taaaatcatt tgcgcgatat cttcatcttc acgtggaaga aattcagaat gggcatcaag
     4981 aacgacaact ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc
     5041 agccccgatg atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc
     5101 aatcaagtag cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt
     5161 attgatgaaa atccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat
     5221 ttgatggtta gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct
     5281 gatcatatga taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc
     5341 ctctgtaaag tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc
     5401 gatgttgata catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc
     5461 accttttgaa gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat
     5521 attttgatat ttttccatca tttttccctc gcttcttttt tacctaccga ttggtacaaa
     5581 aatatgataa ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac
     5641 cagacaaaaa gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa
     5701 caatcgttac ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat
     5761 gcgcatcttc tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta
     5821 atactacacc gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta
     5881 ctgtggactc tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac
     5941 aagaagcata acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg
     6001 gaggagtgat tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc
     6061 catggttgat cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc
     6121 atttgctact tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct
     6181 gcttattcga ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta
     6241 ctcgtgaaca acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct
     6301 tttatatgat tgattttaag atacatatat accagaaagt tgaccccata aaccaatact
     6361 gttaaaaaaa taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag
     6421 cactcgattt aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact
     6481 ggtttattct gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt
     6541 tactaacgcc aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg
     6601 ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata
     6661 tcgaatacct tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg
     6721 cttcatttct tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa
     6781 tcggatttct tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat
     6841 accttttctt tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc
     6901 atccagaaaa tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt
     6961 atctttccca agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat
     7021 ataacgaact tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg
     7081 ggaattcgtc acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc
     7141 gatcaaatta tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac
     7201 atctgtatcg aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact
     7261 ttccatcgct tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc
     7321 caaaaattgg gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa
     7381 tccaccagca ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc
     7441 tagtgttcca atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag
     7501 gtctgctttt tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat
     7561 aaaagcaata tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata
     7621 ataataggga agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa
     7681 ttgagtggac ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc
     7741 ttgttctttc agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc
     7801 atgaccaatc ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat
     7861 ccagtttgct agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata
     7921 tccttcaact ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc
     7981 tttcatataa aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc
     8041 tgtgcttctt cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg
     8101 acaacttcag ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt
     8161 tcagtcaata cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt
     8221 gctgtacgca aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt
     8281 actattttgt tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc
     8341 gttgcttgag tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc
     8401 ccaataggtt gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata
     8461 ttagacacct ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt
     8521 attttttact tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg
     8581 caaaatcgtc ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt
     8641 gtgttcttta gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa
     8701 cgttgctggt tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc
     8761 catctttttg aatactagtt gttctttcaa gtcttccgga agtttgctag aacgctgaat
     8821 ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga
     8881 aaaatgttct tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc
     8941 ttccagtgga tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga
     9001 catgcgtatt gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg
     9061 attcgggtcg ccgatccctc tgatgctaaa gaatggtttt ttaggaacct ctagtatttg
     9121 cgggcttttt ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct
     9181 tcctccgcga aattccattt agttatttat tatcatacct ctttttgagt gacttggcta
     9241 atcgtgttga cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag
     9301 ataacacgtt tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc
     9361 tcttttttta cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt
     9421 agtacctgca gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc
     9481 catgaatgta actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca
     9541 atgggtaaca acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt
     9601 gaatcgcaat tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg
     9661 tataatgatc gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca
     9721 aggtgcagcg acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc
     9781 cctcaatcga cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc
     9841 aattggcccc acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct
     9901 gtttcttgtg cccgttttga ttctttcttt gattatcggc tggtttgcta ttcccgagat
     9961 ggcagtacaa aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt
    10021 tagtggaagt ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat
    10081 catcggcggt attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact
    10141 gatccgactg tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt
    10201 ttatcagttt ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca
    10261 aggaagtagt ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt
    10321 gctttctcct ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc
    10381 tgggctgatt ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc
    10441 agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag
    10501 caatatgatg accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc
    10561 aatgttcaat accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat
    10621 tatcaattac gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc
    10681 aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta
    10741 ttatctgttc tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt
    10801 ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt
    10861 atcggtggcg ggatcattgg tatgactcta gccagttact tagatacatc aaaattcaat
    10921 gttgctttat ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc
    10981 ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct
    11041 tttttcccta aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg
    11101 acacttttgc ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga
    11161 aaagaagaag cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa
    11221 gcacttccgt tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat
    11281 ggaaaagcat atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc
    11341 caggcaaaag tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga
    11401 gaatacacag ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa
    11461 gaattaggct atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca
    11521 tcttataaga tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt
    11581 cctttcatgg atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat
    11641 ttggctccta ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact
    11701 gatccagatc agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg
    11761 tcagattttt ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc
    11821 ggtctaggct cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc
    11881 ttaaatactg gtcattggtc tacggaagat tatcaaaagg acatctccac ctatattacg
    11941 aaaaatccat catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg
    12001 gattacttct tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt
    12061 tcttttgctt catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga
    12121 atatcaagtc gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa
    12181 tctggtatag gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt
    12241 tgttgcacga ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg
    12301 aaatatccag tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa
    12361 gcttgatagt cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact
    12421 agctcatcta gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat
    12481 gttgcaccaa aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa
    12541 tcatctttca tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg
    12601 ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact
    12661 aggacttttt ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa
    12721 gaaatggccg gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag
    12781 gcagtagcaa aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca
    12841 gagtgacctt ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca
    12901 aatacagcca tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag
    12961 gcaataaagc cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat
    13021 tctccaaagt aacgtaatcc tagcacagcc gctgtattat ggagagcatc aaacaaaatg
    13081 agtccatttg gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc
    13141 tcatcaaagg aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa
    13201 ttcagcaaat aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc
    13261 acagaaaatt cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga
    13321 ctttgataca cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc
    13381 tcaaagatcc gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt
    13441 gtcctaacat cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg
    13501 atcgttcctg gactcccttt tgctagttga taagctgtct tcctgtcaaa aacttcatga
    13561 acattattac cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt
    13621 ccacttgtga tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc
    13681 ctaaacaaaa aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg
    13741 ctgtgggaag caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt
    13801 ttctcggttt catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg
    13861 ttttagcagc atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt
    13921 gattggcagc tgaatagtcg ccgtttaagt atgaagcatc taattgataa cggccgatgt
    13981 aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg
    14041 cagagcttga agttggcgct gttgcagcag ctggggcagc aggagctact tcaactgttt
    14101 caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt
    14161 ctgttgtttt gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt
    14221 caccaacata aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca
    14281 ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg
    14341 tataaacttc atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag
    14401 caaaagttgt acctaaaaca attttcttaa atgaattcat attaaactcc tttttttctc
    14461 cgttttttat tctttcgacg ctattaactt tatcatttag aaatatttca taggtaaaag
    14521 aaacatgaca tttaatgaca aaataacgaa ttatttaata tttgtaacat ttaaacaaat
    14581 atttttttga tacaaaacca taaaagcctt gttgtataag caaagtaatc gtttttctat
    14641 gtttttttat ttttgaatga aaaacgcaga ttctgtaaca ttcactgcaa taatccataa
    14701 aaaaaagcaa aaaaattttt gatttttatc aaattctctt aaaaaaatgt aaaaaataca
    14761 aaaaaaccag taattgttac aacaattact ggacaaaaac tttgaataat gttaactatt
    14821 tttttatttt aactttctcc gcaataattt ctgggcatgt tccaattcgt ttataacgac
    14881 ttcttctgtt tctttagcta atatttcatc taatacatca agccattttt ccggttcttt
    14941 tgttcctaga cgtccaatag cccaagctgc tgttcctctg atgacagggc ggacatctgt
    15001 taaacattct gaaatctcag gcaatgccgt tcgatcacct agattagcta aagcaatcaa
    15061 tgcgtttctc tgcaaaggtt tcttcccacg ccatgaacca gctaaatgcc caaattgctg
    15121 tttgaagtct ttattagaca tcgaaagcat tggctttaat tttggatata cttcgtctac
    15181 ttttggttcc atctcttcat gaaaatgaaa atcttttcct tgattgtaag ggcagactaa
    15241 ctggcagata tcacatccat aaatgacatt tcgcattttt ttacgatatt cttccggcat
    15301 catccctttt gtctgagttt gataagacaa acatcgctgg gcattcattc ttccgtcacc
    15361 aagcaatgct tttgtcggac aagcagtcac gcagcgcata cagtcaccgc aaccaaattc
    15421 tcctggttta tcaggctcaa atacaatatt agtaacgatc tcaccaagat agacaaatga
    15481 accgaactct tcagtgatca acaatccgtt tcgtccaata aatcctaaac cagctcgctg
    15541 tgctacagcg acatcgatca gttctccagt atctacttgc ggagctagac gccattcctc
    15601 ttctttttgc catttttctg ctcttgtttg gatgaattcg atcaatcggt tcaaacggtc
    15661 tctgagtata tcatggtaat cgatccccca agaagcacga gcaaactgcc cgcgtttttc
    15721 atccctaggc acttcttcat ggatcctagt aggatacgca agtgcaatgg aaatgatcga
    15781 tttcggctga tcaaatgtca tttctggata aagacgctca tcgatatttg gatgttcaaa
    15841 gccagaagta tggccagctg ctttttgttc ttccaacgat tcttttaatg aatcaaaagg
    15901 ttccgccgtg gtgaacccga ttttgtcaat tcctaatcgt ttgctttctt gtatgatttc
    15961 ttcttttaat gtcatggtct tcctcgcttg ctgttaacgg ctgtataacc cataatatag
    16021 tttttctttt ttcagctgga caaactggat ctgctgatcg gcaatcttct tttcttccat
    16081 ccattttttt cttgcagcaa atacttccca ataatttgtt gaaattaatt ttcttttgtt
    16141 atctaatgca ttaaaaaaat tcaatgaaac tggttttgta cttaacagtt catggagcct
    16201 tacctcttcc catggcgaaa actcagacag atcgcgggaa tacaatactt cttcatagag
    16261 atttttttgc tgtgtgtacg tgaatgtttg cgggattttc ccaggcctag caatttctac
    16321 tagacgatta taaaacatat tcattaattt gctgttggca ataaattgac ttttataata
    16381 ggccgactgg atctttttga ttactccttc tgctttgatg actgaaaata atgcaggcca
    16441 tgctttgacg gttatttcgt cgttttgttt aactaatacg ccaggtgtga agccgactgg
    16501 agaaaggaac acaaaataac cgataccggt attttcatct aaaggctgaa attcatcaaa
    16561 caaccatttg cctatgtaca gctcttcatc tggttctgtt gtctcatttt gatttgctat
    16621 gaataattta gctaattctg cttcaagaaa ttgaaggtct tgatccttgt cggtattttc
    16681 ctgctggctg atcaatcgaa agacttcttc tttaatcgta tatttatctt tacgtgtaaa
    16741 attcatagcc acaccttcct tccttactct aactataacg gaatttcttc aaaaaaggta
    16801 tctgtacgac gaaaaacagc ataatttttt ttcatatctc ttataaaaaa tgcaaggtct
    16861 gctcctcaag ctagaccttg cacttttcag ctttcttttg tttgtgacaa accttcatgg
    16921 atcgtttcaa tattccattt catcatctga tagtaagaat ctccttgttc tccttctttt
    16981 gctatggaat cagtgaacag tttatcatag attttcaaac ccgtttcttt tgctacccgt
    17041 tccatgcttc ttgagtctac actggtttcc acgaataaaa ccggtacttc ttttgctctt
    17101 atctgatcaa tgatcgcttt catttgatca ggcgtacctt gactttctgt attgatctcc
    17161 catatataag ctgctggcaa atcatatgct cgtgaaaaat acttgaatgc cccttcactt
    17221 gttactaata attttttgtt ctcttcgata gaagcaaaac tttccttcgc ttgtgtatct
    17281 aattctttta gtttttccac atatgcttct gcattttttt tatagagtgt cgcattttct
    17341 gcatcttttt cagagaatat acgtgcgatt tcctccacat attttattcc gttagatagg
    17401 tctagccaag catggggatc tgcttttgta tgttcttcac cgctagttaa ataaagaggt
    17461 tctacatttt tgctaactgc aaaataatct tttccttctt cttttttagc cgtttccatc
    17521 aagttatcga accagctgtt acctgtttca agattcaaac cgttgtataa aataacatca
    17581 gcatcgcttg cctttttgat gtcttctggt agtacttcat attcatgcgg atctgttccg
    17641 actggtacga tactgtggat atctatttga tctgttccga cctcttttgc catgtccgcc
    17701 aagatcgaat tggtagccac gactgacaac ttttctttac ttccttcatc cgcttggtca
    17761 gaagcctgct ttccgcaacc ggctaatacc aagaaactaa ctgccaagat taaaaagagt
    17821 gatttttttg ttttcatcta tctgcttcct ttctgaaata aaattccttt tgttggagaa
    17881 aataaaaaag caaggataaa aaatgcggta catgctaaca cgatcgtagc tcctgatggc
    17941 caattgtaac tataactgaa aaacacaccg acaactgcac ttagcatacc gattcctgca
    18001 gctgtaatga tcattttcag taaatggttc gttagcaagt aagccgtggc ggctggtgtg
    18061 atcaacatgg caatcaccaa gattgttcct actgtctgta aactgactac agccactaat
    18121 gtcaaaaaga acatcaaaag ataatggatc agcgatgtgt tcagcccata agcttttgcc
    18181 attgtcggat cgaatgatgt gatcttcaat tctttataaa agaaaaagac aaatatcaag
    18241 acgattccac taacgagtgc tgtcagcaac aaatcgcttt ccctaacagc taagacattc
    18301 ccaaataaaa tatgatacaa atctgtcgca ctctgggcat atgaaatcaa gataatccct
    18361 aatgcaaaaa aggagctaaa gacaattcca atcgctgtat catttttcaa tcggctccgc
    18421 tgagtcacga aaccaatcac tgctgctgtt agcatcccaa aagcagtggc tccaaagatg
    18481 taactaaaac caaacatata agaaatggct acacctggaa gaactgcgtg agagatagcg
    18541 tctcccatca aagacatccc tcgtaaaata ataaaagaac cgatgactcc agaagcaagt
    18601 cctacaagca tggaagtgac caaagcgtat tgcaggaatt gataattcat taatccatca
    18661 ataaaggatt ggatcatttt ttctctcctc cttttactaa atgtctgatg atctcaccat
    18721 aggccaattg aaggttcttc gatgtaaaga cttcttgtac tggaccggaa gcgatcagct
    18781 gtttatttaa aagaataacc tcatcgaaat attcttctac tttatgaaga tcatgatgga
    18841 cgatcaaaat cgtttttcct gaattcttca attcctgcaa gatgtcaaag atctttcgtt
    18901 cacttaacgc atcaatccct acgaatggtt catctaaaaa gatccattct gctccttggg
    18961 ctagagctct tgcaataaaa actctctgaa gctgtccacc cgatagttcg ctgatctgtc
    19021 ttttggcata ttcttccatc cctacttttt tcaaagcttg tcttgcacgt tctttttctt
    19081 ttttcccagg tctttgtcca attcgtaaag atggatatgt tcctaaaagt acaacgccta
    19141 atacatcaat tggaaaggaa agatccaatt cacttcgctg ttccacgtag gctatttttt
    19201 tacggactga gtttaccggc tgtccagaaa aggtgacttt accagtagta gctggtatca
    19261 actctagcaa agatttcata aatgttgatt ttcctgcacc gtttggtccg ataataccgg
    19321 taattttttg tggatttatc gtcaagttga tagttttcaa aacagttttc ccttgataag
    19381 cggctgaaag gtcttgaatt acaatggccg tacgttccaa atcttacact cctttttagg
    19441 tttacctaac tttttattta aatatagttt aactaaaatg gaaagcattt tcaagtagaa
    19501 tgatgaaaaa gtttttttcg aattttcctc gcattttatg aaaattttct gtagaatcac
    19561 atcaagagtt caataaggct aacctaataa tgaaaaacat gtgttgaaac gtttgattat
    19621 tttttcttgt tgactcaaca acgaaaggag tttttgtatg gaagaagaaa agttaactcg
    19681 aagtcttagt gcccgtcata tccaaatgat cgcactaggt ggaacgatcg gtgtcggtct
    19741 gtttatgggt gcctcctcaa ccatacgttg gacagggccc tcagtgatgg gagcctatgc
    19801 gattgcagga ttctttttgt atttgattat gcgtgcttta ggcgaaatgc tttacgttga
    19861 tccttcgact ggttcttttg caaattacgc gagcgagtac atccatcctg tggctggcta
    19921 cttgaccgct tggagtaata tttttcaata tattgtcgta ggaataagtg aagtgattgc
    19981 tgtaggatct tatatgaatt attggtggcc tgatttgccg gcaattattc ccggaatcat
    20041 tgtcgtcttg tttcttatgc tggcaaatct tatttctgtc aaggcgtttg gcgaattaga
    20101 attttggttt tcgatcatca aagtcataac gatcattttg atgatcattg caggtcttgg
    20161 ggtaatctta tttggatttg gtaatcatgg tcaggcagta gggatttcaa atctttggaa
    20221 aaatggtgga ttcttcactg gaggcgtcaa aggatttttc tttgcattat cgatcgttgt
    20281 tgcttcctat caaggaatcg aactgatcgg aatgactgct ggtgaagcag agaatcctaa
    20341 aaagacgatc attgaagctg tccagtccac tatcggtcga atcttgattt tttatattgg
    20401 tgctattttt atcattgtga gtatctatcc gtggaatcaa ttgagtgaga ttggttcacc
    20461 ttttgtacaa actttttcta aagtcggcat tacatttgct gcaggactga ttaattttgt
    20521 cgtgattaca gcggcattat caggttgcaa ttcagggatt ttcagcgcaa gtcgtatggt
    20581 ctatacactt gccataaatg gaaagatgtc taaaaaattt ctgaaactat ctcgtcacgg
    20641 tgcgcctttt tatcctgtcg tagcaatctc aggtggaatc ttaattggtc tgatcttaaa
    20701 ttatcttcta ccaatgttca tcaaccagtc agaaaatatt ttcgttttcg tatacagctc
    20761 aagtattttg cctggtatgg ttccttgggt agtgattttg atcagcgaga ttcgcttcaa
    20821 aaagaagcac gcagataaaa tgcatgatca tccttttaaa atgccactat ctccttatac
    20881 gaattatgtg acacttgctt ttttagctat cgtattgatt ttcatgttta tcaatcctga
    20941 aacgagaatc tcattgatta ttggactcgt tttcttgctt tacatgacga tccattactt
    21001 tgtacgcgaa aaaagaaccg gaaaataaaa aaagaacgaa gcttcacctt agtggaagct
    21061 tcgttctttt ttattgcttt tatcaattca attttagcgt ctcaccggga gtcaggaaat
    21121 aattctccat gctcatccca ttcagcgaca agatcgtttc tactggtacg ccagttcgtt
    21181 cggctatttg atttggacct tctccttcca aaactacggt tgtcgcagcc tgtgaagtcg
    21241 cgctgttggt attagtttca gaggaatctg ttgtgtcttg tgttgttcga gcttgcattt
    21301 cagaagcttc taccgactct gctgtctcag aatcagaaga tgttgtgtct tgtgtgcttg
    21361 ttgactctgc ttgaagagag gcgctagtct gcaaataaat atcggaaatc ttagcgacat
    21421 gattcgtata attagccgat aattgattga tcaaagtatc taagtctaca ccgtctttat
    21481 tttctgctgc ctgatacgct gcacgtaatt ttctgatccc ttcgtcggag attgcttgaa
    21541 gctcacgtgc tttttggtta gcaactgctg ataaaccagc tattccctct tgatttccct
    21601 gaatttctgt ctggtattct tcaatcaatg caggcgtctt ttcttcgatc tttttcgaat
    21661 aatcatttac gattgcttga agttgctctg gcaaaccatt tgctgttttt tcttcagaag
    21721 tttgtgtctc ttttgtttta gttgatgact cacttgtttg tttcgtctct gttttctttg
    21781 tcgttgtttc cgttatggtc gtacttttcg cttctgacga attttttcca tgaaaatata
    21841 gacccgcaac tcctcctgct atgatcaaaa acacggcaac tgctgcccca atcatgatca
    21901 tccgcttacg agctgcctgt ttttgctgtc ttcttcgggt catccgaaca ttttctcccc
    21961 actcattttc atcatacatt tttttcacca ccaattttta tgtataatag cgttatcaaa
    22021 cgcttcatat tcttattgta gcaaataatt gtcgctgtgc acatttttat ctttgttatt
    22081 tataagaaaa tttcaccagt tgagatgatt ccaagattgt ccgtcgattt ctccttgcag
    22141 ctgcattgtt cgttcgtcta tctcagccat cagtttcttt atttcatata agagcgcacg
    22201 gtcttgatat tctgattttt cgcttaattc atctaattgt tggatcagcc aatcatttgt
    22261 cataatgaag ttcctttcat ttcttgcagt gtaaaggtca agtcttcgat ctgctctttt
    22321 gcctcagtca gtgcttctac gatatatgct tgtttttcag attcttcttc ctgcattttc
    22381 tgattttcct gtactctttg aataaaccag tcaatccgct gagtcagcga tggtttgcct
    22441 tccaatgaga gatactgctg ataagcaatt agcttctccc aacgtttttc ctgggataaa
    22501 tacataaatt gtgctaaaac gaatggcgga agataggtca tatggaattt atttgcgatt
    22561 cgctgatacg gcctcaaaaa ttcggaaatc gtatattctt cttttcctcc agcttggtat
    22621 tctttttccg ccacacctgt tgtcaatacg attcctagtt ctttcccttt taatacatgt
    22681 gctgctcgtt ccaagacatt atcttgccaa atttttaaat gagcaggaga actataccag
    22741 taaaacggaa actggaaaat gatccgatca tgctgggcca agagctgtag ttcagtctct
    22801 atatctattt ttccgtctgg ataacttgat tccaaatgat gccaagtagc tggtaaacca
    22861 gcagaagctt ctttgaaaaa ttgctgattt aaagattctt cgatcgttgg atggctaatg
    22921 atgataagtg tacgcatccc ttttcctcct ttttagtctt cgctttacag tgtagcataa
    22981 ccgccacaag tttcttttgc cgatgcttgt cattttaacc agactaatca ggttgtccaa
    23041 gaaactgcgg tttgtttttc caactgtttt ttcaaggtat ccaaagcaat attcttaaaa
    23101 ttttcgtttt tcatacgttt acgatagttc gtagccgttt ctccggtact ctttttgaac
    23161 attcggctaa atacgactag actattgaac cctacttgat aggcaatact ttccacggat
    23221 tcatccgtca gagaaagaag aaacgcccct cgattgatcc gtaccccgat caaatattcc
    23281 ttgatactgg tacccatatg atttttgaat actcgcgaca aatggctccg attgaccgct
    23341 aaatgctggg cgatttcctc aacagacaat ttttcagcat aatgtgcctc gatgtatttg
    23401 actgtttcaa cagctaaaga ggaatgaacc ttttgtacac caccgctgac ctttccgcca
    23461 tcttttaaaa ggactgataa aaaacggtag gttaatgctg ttaactgtaa ttcattttcc
    23521 aaggtttctt ccgtatcttt catgcattct aaaataatgt cgatatattt ttgtatgtca
    23581 gaagagacca tcgtgtattg gtcttctctg aaaagcgact gccgaatgat ttcttctgca
    23641 gcactcccac caaatgaaag ccagcaatac atccacggat cttctccatc tgataagtaa
    23701 aacgtcgagt ctcctggacg aatcaagaac agatcacctt ttttcaattg gaattgttga
    23761 tttttcacat gataagatcc tttgccttcc atcacgacat gcaggatgta atcattgcga
    23821 atagcaggac caaaagaatg aaacggcatc gttttagaga aaccacaaaa atcaaaatac
    23881 aattcttttt ctacttgttg atgttccttt tttaaaaaaa tcgattgctc cacgtctatc
    23941 cctccttact cctttagtat aatgcaacaa aaagttaaat aaaagtcaca gaaatatatt
    24001 aaaaaggaag cgatttcttg ttaagtttaa gtcattaaaa gatctttgtt attcgttgta
    24061 accggtttac ttagtattat atattgaaca ggagaggaat acagattatg aaaagcaaaa
    24121 tgatccaacg tgtgtcctac gctttaggcg cttttggaca cgacacattc tatgttacat
    24181 taagcactta ttttatggta ttcgtgacaa gccagatgtt cgcaggaaca gataaggcaa
    24241 cgaatgcaaa aatgattgct acagttacaa gcttagttgt aggtatccgc ttagttgaaa
    24301 ttatttttga tcctttgatt ggtggcatca tcgataatac tcgtacaaaa tacggtaaat
    24361 tcaaaccatg gttagttatc ggcggcgttg ttagttccgt actcttggta gcaatcttta
    24421 ccaatttctt tggcttagct actcataata cgactttatt tatcatatta tttgcaatcg
    24481 ttttcatctt attggatagt ttttattcat ttaaggatat cgctttttgg tcaatgattc
    24541 cagctttgag tacggaaaca aaggaacgtg aaaaatttgc tacgtttgct cgtttcggct
    24601 cttctctagg tgcaaatggt actactctag tcgttgtccc tatcgttact ttctttacct
    24661 atcttgttac tcataaacat attgaaggcg cttcaggctg gttctggttc ggtgtgattg
    24721 ctgctgttat ttcaggcggt actgcgatta tcactgcctt gggcacaaaa gaagtcgata
    24781 ctgcgatccg tcagcaaaat gaaaaagctg ggatcaaaga tgtgttcaaa gcagttgctc
    24841 aaaatgatca gttgatgtgg cttgcccttg cttatatcgc ttatgcgatt gccaatgtag
    24901 caacaactgc cgtattattc tacttcttca aatatgtact tggccaacca actgaatttt
    24961 ggatcgttgg tctagttgct gccttacttg gtttagtagc tgttccacta ttcccaatgc
    25021 ttacacgcgt catctctcgt cgctacgttt accttggagg gatcgcaatg atgatttctt
    25081 cttacttttt ctttacgatc gcaggttcaa gtttgccaat cgttatcatc gggctagtat
    25141 tgttctactt ccctcaacaa ttgattttct tatccgcttt gatgacgatc acagactctg
    25201 ttgaatatgg tcaatggaaa aatggtatcc gtaacgaagc tgttactcta tcaattcgcc
    25261 ctttactaga taaaatcgct ggtgctttgt ctaacgggat cgtagggttt gtcgctatcg
    25321 cttctgggat gactggtaat gctactgctg ctgatatcac tgctcatggc gtaacgactt
    25381 tcaaagctta tgcattctat gctcctgctg ctttgatgat tttctccgct attatttttg
    25441 cttggaagat tacattgaca gagaaaaaac atgcacaaat cgttgaggaa ttggaaaaca
    25501 aattggctcc agctgataca atggaagatg aacttcacga tgcagtagtt ccttcccatt
    25561 aagaaaggat cttgaaaatg aagattacag aaaaagattt tggccaaggt tatcacttga
    25621 taaccttgga gaacaaaaac aagctagcac tttctatctc tgatttaggt gctagaatcg
    25681 tcagtttaaa atcaaatgat cgagaattag ttttgggatt tgacaccgct gaagaatata
    25741 tcgaaaaaga tccgtatatc ggtgcttcta tcggtcgcac cgcagggaga atcgaaaatg
    25801 gccgcttttc tctcaatgga aaaacttacc aattagccac agatccaaaa actggacata
    25861 atctgcatgg tggtgctcct ggatttgaat taaaaaaatg gtcttatgtc attttgaatg
    25921 gcgaaaatga agcttctgtc atttttacta ctacaagtcc cgacggagaa catggttttc
    25981 caggtacaat ggatgtggaa atccgctata ctttgacaaa agataatatc tggcgagtga
    26041 cctctcgcgg caccagtgac caagatactt tattcaatcc gacaaatcat gtttacttca
    26101 acttgacggg agatgcttct cagtcaatcg atcaacatga gctttggctc aatagcgaag
    26161 cttatgctcc tttgcgaaca gatagtattc ctataggggt aaaagaaaat gctgcaggaa
    26221 gtgctttcga ttttcaaata cctaaaaaat tagcgtccgt ttttgctagt gatttggatc
    26281 aaaaaaatct tgttgacgga atcgatcatc catttttctt aaaagaaacc ggcttgggta
    26341 aagaagccgc tcgcttgact agccccgata aaagaatcca agtaggtatt gctacagatg
    26401 cttctagtgt ggttattttc actgcaaatt tcggcacaga aacaccagaa atgcgcaacc
    26461 gaaaactggc acatcacggt ggcatcacat ttgagactca aacagctcca ggtgcagagc
    26521 gcttctcagc atttggttcg attcatttaa aagctggatc agtatttgaa acagtgacag
    26581 aattcaaaat caaaacaaga aaggaataac tcatcatgga aatcaaaaga agcttaaaca
    26641 caaaatttgc cgaaacattc ggacctaaaa aaactacaca atacttctca ccaggacgca
    26701 tcaatttgat tggtgaacac acagattaca atggaggcca tgtcttcccc gcttccatca
    26761 cttatggaac gcaaggtgta gcagcacctc gagacgataa taaaatatta gtttattcca
    26821 caaactttga cgatatcggt gtcattagtt ttactttaga tgagctagaa tatgataaaa
    26881 aagcaaactg ggcaaattac gtaaaaggaa tggtccttaa attaaaagaa gctggacact
    26941 caatcgatca tgggtttgag ttgttagtgg aaggaacaat ccctaatggt gcaggacttt
    27001 ccagtagcgc ttcattagaa ttattagtag gtgttgtatt agaagatctg tttgatttaa
    27061 aaacagatcg cttggaacta gtccaaacag gtaaaaaagt agaaaatgaa ttcatcggtg
    27121 tcaattcagg tatcatggat cagtttgcca tcggcttcgg tgagctagat catgcaatct
    27181 tattagatac aaatacgctg aaatacgaaa tggttccagt gaaactagat ggttatgcaa
    27241 tcgtcatcat gaatacgaat aagcggcggg aattggcaga ttcaaaatac aatgaacgcc
    27301 gtagcgaatg cgaagaagcc ttggcacgtt tgcaaaaaac tttgcctatc tcttctttag
    27361 gagacttaga tgaagaagaa tttgaatcaa ctattgacca aatcggcgat gatacattga
    27421 tacgcagagc tcgccatgcc gtttctgaaa atcagcggac tttgaaggca aaagctgaat
    27481 tagaagctgg gaacttagaa gcatttggtc aactgttaaa tgattctcac cactctcttc
    27541 gctatgatta tgaagtaact gggatcgaat tagatacact agtagatgct gctcaaaaac
    27601 aagaaggtgt cttaggtgca cgaatgacag gagctggatt tggtggctgt gcgattgctc
    27661 ttgtcaaaga agaaaatata cctgccttca aaaacaatgt ctatgatgaa tacatgaaag
    27721 ttgtcggcta tgctccggaa ttttatgttg cacacattgg aaatgggaca acaaagatcg
    27781 atccagaagc tgttcatgtt tgataaaaaa atcaacgatc tcagctaaaa acaaatagat
    27841 aaaaataacc tctcagcaat ttccaaatgt gagccttttg ctcgtatttg aaaattgctg
    27901 ttttttattg acacggaaag agaacgtgtg ttctaattgt gttgaagagg tgattgttat
    27961 gtgggcgaat caaaatccgg tatttgttta tgaaaaagaa ccttcaagag atattttatg
    28021 tattgactgc aaatcttttt acgcctccgt agagtgcgtt gaaaaggatc ttgatcccct
    28081 tacaactaaa ttagtagtca tgtcctatcc gtccgattcg acagaaacac ggggtagcgg
    28141 attaatcttg gcttccagtc ctgcagcaaa aaaagcttac ggcatcacca atatcagccg
    28201 tgccagagat cttcccttcc cctatccaag tgatttatat attgttgccc caaggatggc
    28261 ctattatatg gaaaagaaca gagaaatcaa ttctatctat aaaaaatatg cagacgaagc
    28321 gaaccatcat gtctattccg tcgatgaaag cttccttgat gtaacaaata gcatggcact
    28381 ctttggtaaa acaaatgcgt acgacatggc taaactgatc cataaagatg tgtttgagca
    28441 gaccggaatc tacacgacta taggaattgg cgataacccg ctacttgcta aactagcctt
    28501 agacaatggc tcaaaagacg gaaagcaaat gtttgctgaa tggcgttatg aagatgtacc
    28561 agaaaagtta tggaaaatct cagatatcac tgacttttgg ggaatcggca aacgaacagc
    28621 aaaacgtctg aaacaaatgg gtatacattc tgtttatgat ttagctcacg cggattatta
    28681 tcacttgaaa agcaagctag gggtcatcgg tactcagctt tatgcccatg cttggggcat
    28741 cgatcgaagt tttttgggac aagaatatac accaaaatca aaaagtatcg gaaatagtca
    28801 aatcctagat agagattata caagacgaga agaaatcgag atcgttatca aagaaatggc
    28861 cgaccaagtc ggcactcgct tgagacgatc tgcagcaaaa acacaagtag tcagcttatg
    28921 ggtaggatac tctttaggct atcttgatcc aaatggcaag accgggtttc accagcaaat
    28981 gaaaatcgaa gcgacaaatg ctagcagaga tctagcagat gctttattga ccattttcca
    29041 ccagcattac cactaccagg atattcggcg tgtaggcgtg aactgttccg acctcgtata
    29101 cgcgacaggt ttacagctta acctttttga agatcctgaa aagcagataa atgaagcgaa
    29161 agttgatttt gttgtcgata cgattagaaa aaaatacggc tttcaatcaa tcatccatgc
    29221 gcatagtctc ttgcaaggag gacgcgccat cgcacgtagt tctctcgttg gcgggcatgc
    29281 aggtggtatg agtggattag aaggtggaaa agatgagacg gacagcaaaa aaattcattg
    29341 actataacga gtatcgagat cggccatttg gattaaaatg ggggactgct tttgcgatgg
    29401 aagaactagt caacggtatc aaaaaaaatg aagaagaagc acgcaaacag cctcttccta
    29461 aacaacaaat gacattgcaa gaaatcgata gtatactgac agaagccttt ttatctcgta
    29521 aagcagtaat cattcagctc aatttaaaag atgaatttgg aagactcttg gatgaggtcg
    29581 aagggcactt cattggtgaa gcctatgaag attactttat gattgaccat cagccgatat
    29641 tgtgggaaga cgtacggtat attgagttaa aggaaacaaa aaaatggttt gtgacatgag
    29701 aggttgtgac agaagtgatc aacttcaaaa aataagaaag gatttttcga aaattgcttc
    29761 ttaaattttt gtggaatttt ggcttatttt tcgaagaagc aacttctgga acaacgtata
    29821 ttagatatca gaggttgtga aaaagtcgct tagttttgag taacaagcca aggcgctgat
    29881 ttcacaacct ctgtctatct atccatctat tcggtaagat tcatgcatga cgtcttcttt
    29941 atttagtatg tagagccctt tttcttctga ccattcaaca taaatcagct gttttacatc
    30001 tgcatagcct tctttcttca agttctccat aagccatgct tcagtcaatt gattggactg
    30061 aagcacttta tgctgaattt gcccttcatc tacaagcagg atcgataaag ctttgtcttc
    30121 atctgatttt ttcaatacac tcatttgacc agcattctct aggataacat gtttggcatt
    30181 ttcaagagaa aagcattctt gctgtcttaa gattgctcgc aactgctcca tctcgatatg
    30241 gttatttgtc aactctgtca tgttgatgac gccgtctttg attagaacag atggctcgcc
    30301 tttgacccaa cgattgacct tttcattttt ttggacgata cgttcgatac cataaatcat
    30361 tactgcccaa agcgcaatgg caaataggac atgccctaca tggacattgt catcataggt
    30421 tgattcttct aagatcccac ctaaaacaag cgtatagact aaatcaaacg gtgtgatttc
    30481 tgacatggat ttcttcccaa gcaaacgtac cactaataat aaaccgatca acccgaacac
    30541 cagtttcatt gtaattgaaa aaatcatttt ttctccccct aactatctgt taattaaatt
    30601 atatatgctt tctcacgaac acgcatggaa tatacaagga agaaatgtat ccttttacta
    30661 aaaaaaggac tgtgacactg tagtgacccc caaaagttag acttttttgg agtggagaaa
    30721 tccactcctt ttttttatgc tctaaaatga tatttttaca caaccgttac ggttgtttta
    30781 cgaaattgga cg
//
LOCUS       DACDKJ010000031        28895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDKJ010000031 DACDKJ010000000
VERSION     DACDKJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.031"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..1274)
                     /locus_tag="IV328_001533"
     CDS             complement(12..1274)
                     /locus_tag="IV328_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937677.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1267..2166)
                     /locus_tag="IV328_001534"
     CDS             complement(1267..2166)
                     /locus_tag="IV328_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937678.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2311..4089)
                     /gene="uvrC"
                     /locus_tag="IV328_001535"
     CDS             complement(2311..4089)
                     /gene="uvrC"
                     /locus_tag="IV328_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8937679.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4271..4585)
                     /gene="trxA"
                     /locus_tag="IV328_001536"
     CDS             complement(4271..4585)
                     /gene="trxA"
                     /locus_tag="IV328_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8937680.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4672..7032)
                     /locus_tag="IV328_001537"
     CDS             complement(4672..7032)
                     /locus_tag="IV328_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8937681.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7164..7694)
                     /locus_tag="IV328_001538"
     CDS             complement(7164..7694)
                     /locus_tag="IV328_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8937682.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            complement(7752..8189)
                     /gene="zapA"
                     /locus_tag="IV328_001539"
     CDS             complement(7752..8189)
                     /gene="zapA"
                     /locus_tag="IV328_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8937683.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8391..9308
                     /locus_tag="IV328_001540"
     CDS             8391..9308
                     /locus_tag="IV328_001540"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8937684.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9564..10475)
                     /locus_tag="IV328_001541"
     CDS             complement(9564..10475)
                     /locus_tag="IV328_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8937685.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10878..11456)
                     /locus_tag="IV328_001542"
     CDS             complement(10878..11456)
                     /locus_tag="IV328_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8937686.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11476..12144)
                     /locus_tag="IV328_001543"
     CDS             complement(11476..12144)
                     /locus_tag="IV328_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937687.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12159..13229)
                     /locus_tag="IV328_001544"
     CDS             complement(12159..13229)
                     /locus_tag="IV328_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937688.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13400..14764)
                     /locus_tag="IV328_001545"
     CDS             complement(13400..14764)
                     /locus_tag="IV328_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8937689.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14761..16320)
                     /locus_tag="IV328_001546"
     CDS             complement(14761..16320)
                     /locus_tag="IV328_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8937690.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDCPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16376..18856)
                     /locus_tag="IV328_001547"
     CDS             complement(16376..18856)
                     /locus_tag="IV328_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937691.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18840..19175)
                     /locus_tag="IV328_001548"
     CDS             complement(18840..19175)
                     /locus_tag="IV328_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937692.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19189..20223)
                     /locus_tag="IV328_001549"
     CDS             complement(19189..20223)
                     /locus_tag="IV328_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8937693.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20313..20993)
                     /locus_tag="IV328_001550"
     CDS             complement(20313..20993)
                     /locus_tag="IV328_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8937694.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21277..22380)
                     /locus_tag="IV328_001551"
     CDS             complement(21277..22380)
                     /locus_tag="IV328_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8937695.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(23009..23479)
                     /locus_tag="IV328_001552"
     CDS             complement(23009..23479)
                     /locus_tag="IV328_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8937696.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            23809..25449
                     /locus_tag="IV328_001553"
     CDS             23809..25449
                     /locus_tag="IV328_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8937697.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            25603..26925
                     /locus_tag="IV328_001554"
     CDS             25603..26925
                     /locus_tag="IV328_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8937698.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(26993..27427)
                     /locus_tag="IV328_001555"
     CDS             complement(26993..27427)
                     /locus_tag="IV328_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8937699.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(27492..28329)
                     /locus_tag="IV328_001556"
                     /pseudo
     CDS             complement(27492..28329)
                     /locus_tag="IV328_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT         8330 a         5998 c         5006 g         9561 t
ORIGIN      
        1 ttttctatat tttatgcttt ttttcgttca tttctcataa ttgaaatcga ttgtttcaat
       61 gaactccata atccgccttc gctatacacg agtacattcg atttgtacat gtttgcagac
      121 aagatcatca ataagaatgt tacggctaca agtaccaaca aggaaatcaa tgctccaccc
      181 ataccgactg tctcatttgc taagcgaacc ggcataatat atgaagataa gaatggaata
      241 tacgaagtga tccgaataac gatattgttt ggatctgaag ctcctagtat caatccaagc
      301 atatatccgc ctagagatag ataagtcact ggcaaaattg cttttgctgt atcttctgct
      361 ttgttgacca aagagccaca taaagctgct aaaacagaaa aaatcaaaat gccaaagatc
      421 atgaatagaa gagaatacca caagaatggt ccaatcaagt tatccaatga gataccgtct
      481 aatatatttt tgaccatttc catatctttg aattgactgt atcctatacc aaaaataata
      541 ccgtataatg ccatttgcgt taatgcaaca agtaaaacac ctgttagttt tccgtaaaaa
      601 tgcttttgtg cagtcgtact ggacaggatc acttccatga tccgcgtgcc tttttcagaa
      661 gcgatctctt gtgcaatgat ctgcgcatac gtcaaaataa taatgaacaa gacaatcgtt
      721 gctacataac ttacgacata ttggactgtc gagttatctt ctccaactgt catttttcct
      781 tttgaatcaa aattgacttt ttgtcgagtc aaacttgctt gttggctcaa gctagctact
      841 tcttctggtg aaatgcctaa ttgacttgct cgaagcatcg attgcaagcc agtcagttgt
      901 tgttgtatca gtagttgtgt actttgtcct aatgaatttt cgctataaag catcccattt
      961 atcttatcat tctcagttga gactactaaa tacgcatcga ttttttcttc cgaaagctct
     1021 ttttgtgctt tttcttcaga atcaactgct ttgaactcaa aatcatctgt tttgacttgg
     1081 ctcatttgct gactgatttg ctcattatca gccaatacgc cgattttatt gacatcacta
     1141 ttttgttgag caaagtttcc tgctacataa atgataccca gcatgataaa agggactaat
     1201 atcatgatca agaaagaaat cgacttaaca ttttttaaat agacatcttt ggtaataatc
     1261 caaaacttac gcatcgattt cacctgcttt caatttgaaa atctcttcca aagttggcgg
     1321 ttgctggtta aacataggaa tgtagccgaa ctgagtcgca cgagtaaaga tttctttccc
     1381 agcttcagga tcttctaagg taatctctaa cgtattgtct tcatgaagga tcactttttt
     1441 cacgccttcg atttcttcta cttcctgttg gcttaaacca gattccaaga ataacttcgt
     1501 acggccaaat gattcacgaa tctcatgtac ttttccattc aaaaccattt ttccgtttcg
     1561 caacatgatc aaatgatcgc agattttttc tacattatcc atattgtggc tagaaaaaat
     1621 gacacatgaa ccatgttttt tcaattccaa tattccatct tttagcaatt cagcatttac
     1681 cggatctaac ccgctgaatg gctcatccaa aatgatcaat ttaggttcat ggattaacgt
     1741 ggcgatcagc tggacttttt gctgatttcc tttagacaaa gattttactt tatccgtctt
     1801 tttccctttt acctgaaatt tttccatcca aaaatcgatt tttggttcaa tctcttttct
     1861 tgatttccct ctaagttctg caaaataaag cagttggtct tgaatcgtta ctttcggata
     1921 aagtccccgt tcctccggaa gataaccgat gacatcataa tctttctccc caagatgatg
     1981 gccattccat aacacttctc cttggtcttg tgtcaaaaaa tccaagatca aacgaaaagt
     2041 cgttgtcttc ccagccccgt tttggccaat tagccccatg atttttccat cggggatttg
     2101 gaaagagaca tgatcgacag ctgtgtagtt cccaaaagtt ttaacaagat ccttgacttc
     2161 taacatttct tcactccttc ttcaagaaat cacatatatc cttagacacc agacgtaagc
     2221 gatttattat ttaccaacaa aacgtaaacg ctctttttgt tgtacataaa atgataggac
     2281 actttacttt ctttgtaaag tgccctttcc tcattcctga tgtaatttat caaatacgtt
     2341 ttgtgcgaca tttttcggca gtccgatttc ttgtaattct tctgtcgtgg ctgctgtgat
     2401 atttttcaac gatttgaact ctttaagcag catttttttt cgttttggtc ctaacccttc
     2461 aatttcatct aatcgggaag caaaactgtt tttactgcgt agctgccgat ggaaagtgat
     2521 tgcgaatcga tggacttcat cttggattcg ctggagtaag aaaaactctg gtgaatttcg
     2581 ctcaagcggt actacagcca aatctatacc gaatagcaac tcatttgttt tatgtttatc
     2641 gttttttgcc atgccagcaa taggaatatc gattcctaat tgattttcta agacatcttt
     2701 tgccacatct acttgtcctt ttcctccatc gatcaagatc aaatcaggta aaggcaaccc
     2761 ttcgcgaatc actcgtgaat atctgcgata gatgacttct ctcattgatg cataatcatc
     2821 cggtccgttg actgttttta ttttatattt ccgatattct tttcttgccg gttttccatc
     2881 aatgaatacg accatcgcag ccacaggatt cgtccccatg atattagagt tatcaaaaga
     2941 ctcgatacgt attggggtcg ggatattcat cgcgtttcct aaacgttcaa ctgctccgat
     3001 cgtccgttcc tgttttctgg caatcagatc aaaccgttca ttcaaagcaa ctgaggcatt
     3061 cttattcgcc aattcaacta attttttctt ttctcctcgt tttggttgca gcactttcgt
     3121 agctaacaaa gcttctacac tgggcttgtc gatcgtatca ggaatcaaga cttctttagg
     3181 aatgaaatgc tcattttcct gataaaactg accgaggtaa gtcaagaaat cttcttctgc
     3241 ttcgttatag aaaggaaaca ttgaaacatc tcgttcaatt agctttcctt gtcttacgaa
     3301 aaacacttgg acacacatcc atcctttgtc aatcgcataa ccaaatacat ctcgatccag
     3361 taaatcagta tttgtcattt tttggcgagt catgattgtc tcaatggcac ggatctggtc
     3421 tcggtattct gcagcttttt caaactccat gttttcagca gctttttcca ttttctgatg
     3481 gatctctgac tcgatttttt cgtgtcctcc gtttaaaaac tttttcactt catcaacgat
     3541 tttcgtatag actttcggat ctacgtcgaa ataataagga cacagacatt gtcccaaatg
     3601 atagtaaaga caaggctcct tcgtctgact aggtttgcat tttctcagag gaaataatcg
     3661 atcgaggatc ttctttgtct cattcgccgc accgacatcg ggatatggtc caaaatacag
     3721 tgctttgtct tttagtactt tacgagtaat caccaaacgg ggatattttt cattcgttat
     3781 cttaaggaaa ggataggttt tatcatcctt taacataatg ttgtatttag gatcattttt
     3841 ctttatcaga ttgatctcta agagcaatgc ttcgatattc gattctgtaa cgatgtattc
     3901 aaagtcttcg atttcactaa caagccgttc cgttttcgta ttatggctgc cagtaaaata
     3961 tgagcggaca cgatttttta atattttggc tttgccgaca taaatgatcg ttccattttt
     4021 atctttcatc aagtaacagc cgggctgatc aggaagtaaa gctaatttgt ttttgattcg
     4081 ttcgttcatc tggtactccc cttcatattc aattaaagtt attatagcat gtctgtcaaa
     4141 aaaacaatcc agcctgacaa cgtaattata ctcaaaaaat attaatttga ataaccttaa
     4201 ctcaaccgca aaaaaaccga ggacggaact ggattcagtc ccaccctcag cttatttttg
     4261 ctgtaagttt ttataaatat tgatcgatca tttgacgtag ttgttctttt gaatgaacac
     4321 caacagcttt ttctactact tgaccatctt ttttcaacat taatgttgga atgctcatga
     4381 taccgaattg ttgaggagtt tctggatttt catccacatc cattttagca attctgaatt
     4441 cttcttcatc atattcttgt tccaactgat ccaaaatcgg tgcttgcata cgacatggac
     4501 cacaccaagt tgcccaaaaa tcaatcaata caagtccttt atctgtttct tcattaaatg
     4561 tcttatctgt aattacttgt gacatatcaa agacctccat ttttaaaatc tatgattagt
     4621 atagcatttc tatacttgga tagcgaacca tctgcttacc tttagtaagc tttacttaaa
     4681 tttaacaatt gtcgcaccat ttccgccttg gtttgccggt gcaaactcaa agcttttgac
     4741 gctccgatgg tttttaagat aatcagtaat tcccttgcga agtgcgcctg ttccttttcc
     4801 gtggacaata gtcacttgag gataaccagc tagaatggca gaatccaaat attgatctac
     4861 ctcacttagc gcttcttcgt atcgttttcc tcgaaggtcc aattgattcg gcacgtgact
     4921 actagattca gatcgtacag catggataac tcgttgtgtc ggctcttttt gaggagcaac
     4981 aggcgtcagc tcgtcttcgg acacattcat ttttaatata ccgatttcta cttgccactg
     5041 attcccgttt ttacgtaaaa gcgtccctct ttggccgtaa gtcgtaacca acacttcgtc
     5101 tcccggtttc aacgtttttt gttctttcgc cttcttcaat actttatttt tcttcaaagt
     5161 ttcttcttga tgtagatttg ctaattgggt ctttgcatcg atcaactgat gctctttcac
     5221 atttccttgc ccaatctgct gttgcatttt acgaatatca gcaatgattt tttctgcttt
     5281 ttcttccgcc tctgacacga cttcgtttgc ttttttcttt gctttttcca tttctttttc
     5341 acgttcttca aagaaatagc tgtaagcttc tttcaaatca tcgtgaagtt cttgtgcctc
     5401 agaaacaaaa tgacgcattt ccagatattc ggtctctgcc attttccggc gattctctaa
     5461 atctgtgatc atttcgttta aatcttggct ctcatcattc atcaactgct tcgcttcatc
     5521 aataacttct gtatcaagac ctaatcggct agagatttca aacgcattgc ttcgtccagg
     5581 aacgccaatc agcaaacgat acgttggact tagtgtatcc acatcgaact ccatgctggc
     5641 attaatcgta tttgcccgat tgtatccata aactttcaat tcaggataat gcgttgttgc
     5701 catcacataa gcagactttt taccaagatc atctaagatt gcgattgcta gagcagcacc
     5761 ttcttgcgga tctgttcctg caccgagctc gtcaaataat accagacttt tttcattgac
     5821 ttttgataaa atatctacag tgttcgtcat atgagaagaa aaggtcgata gactttgctc
     5881 aatcgactgt tcgtctccta tatccgcaaa tacttcctca aaaataccga tttgactttc
     5941 ttcacctgca ggaatcggta atcctgcttg ccccatcagt tgtaacagac ctaaagtctt
     6001 caacgtgatc gttttcccac ctgtgttagg tcctgtaatg acgatggctt gatagtcttt
     6061 tccgattgtg atgtcattag aaactacttt ttcctgatcg atcagtggat gtcttgcttg
     6121 tttcaagacg acatgattgt cttcactgat tcccggcacg atggctttta cttctttacc
     6181 aaatcgtgct tttgcattca tcagatccat tttaccaatc acataagcat tgtggatgat
     6241 ttcttgacga tggggaacaa gttcagcaga aagttcactt aaaatacgtt gaatctcatt
     6301 tcgttcagcg atttggtatt gacgcaaacg attattcaaa tccaccactt gttttggttc
     6361 aataaataaa gtctgtcctg aagcactttg atcgtggact acaccgccaa atacaccgcg
     6421 gtattcttgt ttgactggga taacgtagcg gtcatttcgc atggtgatga tcgcatcact
     6481 taaatacttt gcatttttcc cacggacgat cccatctaac tgttcacgaa cagcttgttc
     6541 acttcgccga atgttctgtc ttatgctctt taactcagga gaagcgtcat cagtcacgcg
     6601 tccgtcttca tcgattgctt cctttaaacg gcggctcaaa acagggatcg tgactaactg
     6661 atctgcccac atatacaaac gtgcaaattc gatttcactg tcagaaagat catcaatgaa
     6721 acgattcaat tcgcttgtcg tagacaatac tctggaaact tgtgcaagct ccactccatt
     6781 caagtctgct ccgatctcga tccgtttcat atggggacgg atattttcaa gtttaggtat
     6841 agggatcccg cctcgcagac gttgcacttt caatccatcc tcagtttctt gaagccagtt
     6901 tgtgatcgtt tgtttctcac tcactggtac caattctgcc agttcttctt ttccttgtgc
     6961 agtcactaca aattgtctga ccatctgttt gactttttca aattccaatg tttcaaggat
     7021 tcggttattc attttatcac cttatctttc actatgaaaa aagagctgtg ccggacatac
     7081 gctacgcagc cttcttaaat aaactactct gtacaaaaaa cgaaatattt tccagcacta
     7141 ttttccttaa ttcaaaatat tcgttaccaa agctcataca tcttgttggt aagaaatggc
     7201 gttctctcaa cgataaaacg agcgaaacta tgctcgccga attgattttg gaccacatct
     7261 aatggaacaa aggcaagtaa ctgcaaaatc aaaaatatag taacgtaagc catgattagt
     7321 gacaggatgc ctcctgcaat ccagtctacc tgtctcaaga tcggcacata cgtcaatcca
     7381 tggacaaaaa cccccacaaa tcgggtaagc agccagccaa ttagcaaaat aatcagaaaa
     7441 gcagttcctg cataaaaagc ttcatctagg tgaaaagcaa atacctgatc aaaaaatgct
     7501 aactttgatg ttggggtgac agctgggtat ggaatgtata attccaaatg acttgctaat
     7561 ggtttgtaaa aatgctgggc agcaataaat gacaatacat atccaatcgc gaatatgatt
     7621 tgcaaagcaa acccgcgacg tgctccagaa aaaaacgcaa ttagcaataa aaataaaatc
     7681 agcaaactca gcatgctacg ttcctttctc ttctaacttt tttaaatttt tcttattttg
     7741 ataaaaggat cttaattaga agatttttgt ttaatttgtc cttttcgttt ctcgtttaaa
     7801 atctgttgtg cttccacatg atttttaatg gtatgatctg cttgaccatt ttctttcaat
     7861 acttcccgag cttcgttctc gattgcttca atgcgtttca cacgattttc aagctctgta
     7921 aatttgatca tttttttctt taattctgct gtttcttctt ccagctccaa gattctttct
     7981 tgtttcttca attgatccga aagcgcattg acagccatca agattgctgc ttgttcatta
     8041 tccatttgtg gtgataattg tttcaattct gctaactgtt cattgatcag cttcgttacg
     8101 atatccatat gatgcttggt ttcccgtccg ataatagtat atgtttggtt ggcaatcact
     8161 gctttgtagc gtgttttttc gtgcgccata cgaatcattt cctcctattt gatgaaagtt
     8221 ttatttagtg taccatgaag ttggttcttt caccgttctc cacaaatatt tttcacatac
     8281 ttaactatta tatgataaac tttaaaaaaa tgctatgtct cgcaatctct tagaaaacga
     8341 aaagtcgtga gttgcggtat agttttatgt ataaaaacga ggagaatttt atgagcaacg
     8401 tcattatcaa agcaacaaac gaccaactta gtcaaatgaa agcatactat cgctcttatt
     8461 tgttaaataa acagattcct tatactagct ttgctgcaaa aaaagacggg acgaccatta
     8521 ccgcttatac atccggaaaa gtgatgtttc aaggtacgaa tgcagcaata gaagctgata
     8581 aatgggggga ggcacaggat aaacaagcca agccagcaaa aacagcctct tctttgccaa
     8641 aaaatatcag tcaattatcc gttttaggta gtgatgaggt cggaaatggc agctattttg
     8701 gtccgctaac agtatgtgct gcttatgtga aacgtgatca gcttgcagaa ttacgtaaac
     8761 ttggtgttag agattcaaaa gagttaaaag atagccaaat catccagctt gctgacatct
     8821 taaaaaaaac catcccctat aaactgcttg tcttatctcc agaaaaatac aatgagatcc
     8881 aaccaaaata caatgccgtt cggatgaaag tcgctttgca caatcaagcg atccatttgc
     8941 tgctaaaaaa aatcgagcca gaaaaaccag aagcgatttt gatcgatcag tttacgccgg
     9001 aagcaaattt taaaaaatat gcgcgtttag aaaaaaatca attacaagaa aaactgtact
     9061 tcgttactaa aggagaacaa tatcatttag ctgttgctgc tgcttctatc atcagtcgtg
     9121 ccagcttttt ggaagagtta cacaaagcat cagctgaagc tggaatcacc cttccatcag
     9181 gagctggaac gaaatctgac caagtagcgg caaaactatt ggagaaaggc ggcatgccta
     9241 tgcttgaaaa atatgccaaa cttcattttg ccaatacaga aaaagctttg aagctgctaa
     9301 aaaaataatg gaactgtgac aaaagtcatg tcacagtccc ttgttccgaa taaacggtgt
     9361 tctcatgcca actcctcggt cacaagtcac aatagtcatc aaacatgaga agctgttttt
     9421 tgaaattgct ccttgtgacg cggagttaaa cggcaggctc acaacctctt ccttaaacgg
     9481 tgttcaaaag cttgcatctg cgataataag cccggccaaa cgaaaaatat gaggagctat
     9541 ttttgtttgg ctgggcttat ttattactga aaataagtgg tcaatgcttg tgtcaaactt
     9601 gcagcaatct gctgctgata actgtctgtt actaattcct ttaaatcact atcattattc
     9661 atataaccca tctcgattaa aatagaaggt tgaagatttt ctcgaagaac ttgatagttc
     9721 ccagtggaga ttcctcttga atccagaggc agatcagtga aggagtcagc aattgtctgt
     9781 gctaatgttt cctcttgtcc atagtaataa tatgtcgtga ttccagttgc ttcattcgca
     9841 tattccgtag agtctgcgtg taaactgata aagacatctg cctttttttc ttcactgtag
     9901 tcgcatatat ctccaagctg tactaattca tctgtagtat gggtcaaata aaccgttgct
     9961 ccagcatcct ctagcgcctt ttttactttt atggctgtac ttaatgttat atctttttct
    10021 tctgtttgtt catcattgct taatgcccca gtatcatttc cgccatgccc aggatctaat
    10081 acaatcactg catcagatag ctcttccggt tttgtttgta tattctttgt gactttggtt
    10141 ccatcttcga gggctaaata tccagtatag ccatcatttg tcttgatctg ataagcattt
    10201 tttgtttcag aaagaaactc cgccagctct ccgccgttta tagttgcaag tggggtagtt
    10261 agcgagtcat ctgtatatag tgtacggtca ttttttccga ttttgattaa tgtctcttgt
    10321 tctgtatcat caaatgattc ctttgtcttt gtaaccgtac tgttcgtact gctgaccgtt
    10381 tgtccaacta tctttttttg accaaaaaca tctatttgaa aacagataat aatgataatg
    10441 attgcaaata caaaagctcc aattattttt ttcattcttg actctcacct cgtagatcca
    10501 gactaagggt caaaacttaa aatcagctta agtacgcaaa aaaggggctg taaaagatat
    10561 aaccgtagaa aataacatta ctgctaaaat gatttttttc atttttggca tatcctgaaa
    10621 tctccttttg actattcgtg gtgattcatc tacccaataa atagtttcct tcattctaca
    10681 aacaattaat ttcttcgtta ttaaattaaa acacttcctg gaacaccgac ataataaata
    10741 aaaatatacc ctgataatca tgagagaata acaaatttgt atttatgaaa aactacctat
    10801 ataaaaaaaa caagatataa cgatcttcgt catatcttgc ttcttcacct aactattttt
    10861 ctccctatat aacagcttta cgcttctcct aatacaccat tcaaatataa atcgatcaat
    10921 tgcgccacat ttagcggttc ttccacaggt cttttcgacc gagtgatgta aggtgctaac
    10981 actaagaaaa aatgccttgc tgcgatttct tttgaaatcc catttcttag cacagattgg
    11041 tttgctgaaa agatttcttc aaaaggttca cgataatttt tcttccatag aaagaaaaga
    11101 tgatctcttg tttcctttga aagattgctt tcaagatcgt gaagcatcag aaacaaatcg
    11161 accagatgtg tctcaatcaa aaactgcgcc atttttgtca aggttacttg aaatcccgtt
    11221 tcgcttgttt gaacgatttc tctcaaagct tttccaacat cttttaagtg gttttctaaa
    11281 actgccgtat atagtttttc tttatcaccg aaataatgat agagattggg ttgggtgatc
    11341 ccaacttttt gtgcgatctc tcttgttgaa gtgccgagat atcccttgga cataaacaag
    11401 tcagtcgctt ctttcaaaat tttttgctgt gtatctttgt tgtatcgatg gaccattttt
    11461 ccacctgctt tttggttatt ctttttcttt caatacacca tcttccatca taaatattct
    11521 atcgcatttg tcaatcagcc gtgtatcgtg agtaaccatg atcgttgctt tatttttgtc
    11581 ttttgattct tttgccaaga tatcgactac ttctcgtgcc ttttctgaat ccaaactagc
    11641 agttggttca tctgctagaa taatggacgg atcattgtac aatgcacgag cgattgcgac
    11701 cctttgccgt tctccaccag atagttcttc tggatacttg ttgatcaact tatccacgcc
    11761 aagctgctca aataattctt ttgcttcttt ttgacgatgt tcatgtttga tcttatctac
    11821 taataataac tgctttttta ctgtcagaaa aggaaccaga ttggaagctt gcaaaataaa
    11881 gccaatttct ttaaaacgta gtttaacgcg ttcactttct ttttgattac tgaattcttg
    11941 atcattgata aatacagagc cactgctagg cgtctgcaat ccgcctgcca aagtcaaaaa
    12001 agtactttta cctgaaccag aaggaccgat gattgcaaca aattcccctt tatcgatact
    12061 catggttgtt tctttcaatg ctttaactac ttggtcgcca tcttggaaac ttttttctac
    12121 ttctttaaat tctattgctt tcatgctctt cctccttctt atccaattgc cttcaacgga
    12181 tcgattttta cgattgtccg aacagaaaat agtgcgccta acacagctac taccagcatc
    12241 aacaagctaa tgacgccaaa aaacagccaa ttgctttgga atggaaccgc atcaggtaga
    12301 acaagggatg ttcccactgt tcctaataga ccaagaagaa taccaacaaa tgataaaatg
    12361 aatgtttgtg ccactacaga acgcgcaata aatcctcctg tgatcccttg tgctttcatt
    12421 acaccaaaga tattaatctt ttgcatcgtc agtacataaa tgaaaatccc gatcacaata
    12481 gccgcaataa cgatcaaaaa tccgatcata aatccaaaag tcaatacttg cgcattatat
    12541 ccaggtaatt cattgatgaa tttagaaatt ttgatctgtt ctaaatcttc aggtaaatta
    12601 ctgattttac ctctgacaac tattgcgtta atccgtgcat tttcacttgt atcttctttt
    12661 tcgaagcgga tctcttggta agcattgatg gttgtataga gaactggtga gacattgaat
    12721 ttggcatgat ccgtaaagcc agttagttta aacgtcttgt cactaccaga taacttaact
    12781 gtatctccta cggctagacc atattcttct ttcaaactaa tatcgcctac tgcctcatca
    12841 tcgttatcaa aagcttttcc ttcaacaaga ttgggcatga tgaattgatt tttatcgata
    12901 ccgaagaagc taacattgat ttttccttct tctgtactgt ctttactagt cacgactcca
    12961 ggtgtctggg caagatacgc cactttccca ccttcgactt cttctgctat cttcttagta
    13021 atcatggaca tccctagatt tgaatttgca tctttcgaaa gcacaatcga atctgcctcc
    13081 cacttgtcga ctgctgttcg attatcttga gctaagccat aagctaaccc agtcaaaaaa
    13141 aataccaggt aggcgatcaa aaacatcacc cctgcaacta acgcataacg taactttgaa
    13201 tgcataattt catttaatgc taaaaacatt taactcctcc tcctgaaata atcattttat
    13261 acaacaattt tatcacttga taaatttttg gcgaatatta tgctcaaaaa attcttccgc
    13321 tatttcatta tatgaatttt ttattctcga ttgattaata tttattatat ccccacacaa
    13381 taaaagaccg tgatatttct cacagtcttt tatctactct attgctattt gctctcttag
    13441 ctagtacttt aaaagctaac tcattgaggt agtgaaaacg agaggtgtgt tcagacagaa
    13501 atccaaaatc ctcaactgct agctgtggaa tcgtagtttt gtgtttcacg atatccgcaa
    13561 gcaggttcaa atctgataat aagaattctt ggcttatcag ctgtccgcca attatttcta
    13621 atgtatccgg atggtagatc agctttgcat ttactgtttt gttttcttca tcgtattgtg
    13681 gaaatagatc aaattctcct gaacacgaac cggtattttg ttccaagaat ggcgcttcat
    13741 cttctgtcag ccctacagtt cctaaaaagt agtctataat gactgctgct gttatctttt
    13801 gtctgaggtc gtaagcggtt ggctgaccac tcaaagttag cgctactgct cttgccatat
    13861 gtacagctcg agtaactaaa ggtaaaaaga gatgccgttt atttttgtca aaactgactg
    13921 ggacgatatc tccaatcgca tagatgtccg gatcacttgt ctgcagatat tcattgacta
    13981 tcacagtctc atcctcatac aaagccactt tttcttttaa caatgtcgta ttaggacgag
    14041 tttgggcaga aaaaatgacc atatctgcct ttattgcttg agatagaagt tgtacggata
    14101 ctactttctc ttctgatttt ttccaatcga tcaagaattc atttggataa aaattgattt
    14161 gcttagtttg cagctgattt tgaaaactgt tgatcatatc cttatcgaaa tatcttgata
    14221 aaacattcgg catataatca actagatgaa taatgtatcc tttatttctc aaagcatctg
    14281 ctaattccaa tccaatataa ccagcaccga agatcactac ttcttttgct tcttttaaag
    14341 cggcaagtac ttgcttagat tgcactagat ttttataggt gtagacattt tcagcttcaa
    14401 tttgtttata caaggtgaca ggagaggaac ctgttgctac gattaattta tcataggtta
    14461 tggtctcgtt ttttccttct ttggatgcga ggatgagctg atgtttgtct ggatcaagtt
    14521 cagttactct ccattcggtg atcagttttg ccccactttc ttgaagagtc cgcatgttat
    14581 gggcaacttc agctaaacta cgtatctttc cttttaaata aaggttgatt ccatttgaaa
    14641 catagcctag tccatcttta tgatcttcat cgatcagtaa aacttctgct ttcggttgaa
    14701 gttttcttag atttagggca gctgtgatgc ctgcatggga agcaccaatg atcacgattt
    14761 tcatgtttta ttcatcctta aattcaattt ttctaaataa cattctttta aaaattggtt
    14821 caaatagggg caatcataat tatattcgtt gctagtgaat acaaaaactt tagcggactt
    14881 ctgtgacggg tactctttca attggctagt caaaatgata tcatactgct tttctttctc
    14941 gtattcgact aaatcatatt tgatcgtatt cagcaattgt tcctggatga tttctttcac
    15001 gatatccgcc attagccttt cataaggaag atcataagca atttgcagac gtaattctaa
    15061 ctgcttgatc agcgtccgca aaatagatgc atagcgagca aataattcaa agcgggcatt
    15121 ttcctcgtct acttcagtaa aatcaacaat agcaaatgtt tgtctgacca atctttcaca
    15181 taaaaccagg aattgtttat cttctacttc agttaaccgc tgttttaatc cttcttggcc
    15241 aaaaatattc atccatcctc taaaataggc aaaatgagcg tgattttgca tcactgtcaa
    15301 cgcaatcttt tgcgaaatct cttctggaaa aacataaaaa gagaaatatt cttctatcga
    15361 ttgtttaaac gttcgattca attgagctac tacgtcgtct ttgtattttt cgcttatcaa
    15421 aaactcctgg acatgcgagt tatgtgtatc taatgtaaag ttactgatca tgaaaagata
    15481 aaaaaccaac gtttcatttt gattccaagg aaaggcatat tgaaagaagt aacgggtcaa
    15541 aaatgtatgg aggtccaata aaaattgtgt cgtttgaaac aatccaacag gaagattgct
    15601 ataatctggt ttggattttt tcgtacagcg cattcgcttc ttcataatgg aaagccatat
    15661 ttcaaatttc aatcgtcctt cttctgtaat agtaaaacta aactcttttt gcagcagatc
    15721 taaaaaagtg tttcctgata aatcactgta ttcttttttg ttttcaagat aaggacgtcc
    15781 aaaccaaaat aattggaaat aaaaataaca aatttgactt tctggaccgg tcagccgccc
    15841 gctttttatt ttcagatgaa actcatttaa agaatcattt aattccttca ctctgcgaga
    15901 caaagtagcc gaactgatat taagttccat gacaagtttc tgccgatcaa atgagccatt
    15961 ttccaacaga taaagaagta tctgatattt tgtagaatag cgcaagtagc aatgataata
    16021 aacatcgact gctgctatat ttccatgtct gattaataaa atattccctt cttggataac
    16081 aatttccaat tgtttgatat tttccaagtt agcttgtaaa taaaacagat cttctttcaa
    16141 ggtagcccga tcaactgata agcgggtaag tacgttatta acggaaatct ctcctttttc
    16201 ctctaataaa agttggaaaa gtttgatttg ccgttgttca tttttttcaa gcaaactgaa
    16261 aagttccaag tccactcctc caagcatttc tgattttaac agcagaagtt tccttctcat
    16321 ccttgaaaaa gaggatctgt gacagttatc acagatccat acaaattcta atcacttagc
    16381 tggttggccc agctgtcagt tcccatgtga ccatgccttg atacgttgcg ccaggttgag
    16441 cattcgtact gctatttacc tctaagagta aaccatcggt atcgggaaag tcaacgagaa
    16501 cctctccttt agctgttcca tcactctcat aaatcaacgt gttttgacct tgctgaatca
    16561 attgttttga tccagaatga ttgaagtata attttgttcc gcttaattct tgaccttgat
    16621 cattcttaaa taaagaagtc aatgtccctg tcagttgcca aggagacgtg cttggaagcc
    16681 gagtatcccg cacatcaaaa gaaaaatcgc cttttgcttc ataataactt gtcttcaacg
    16741 gtttggggag atcgttacca aatgtaaaat catctggtac attaactaat gttaattctt
    16801 gtttggggac aagaattgtt ttagcagatg tttggacaac tgggttagga agcttagcac
    16861 catctgctaa tgtaatattg ctggaaagta cagctgcctt tgtcagtatg ctgacggatt
    16921 gtacagaagt atctacagta tttaattgat agttgtaagt aagatgaatg gtatctccta
    16981 ttttgctcaa tagatctgct gggacagaaa ctccttgttg aatgccggta tctgcgatag
    17041 ttgtaccatt taattttaag gaaccattga tataagtagt attttcagga agtgagatcg
    17101 tgattttttt gtcggttatc gcatccccag attttgttaa cgtgaatgtc acattttctt
    17161 gaatcgtatc tcctgataga gcagacaggg gatctcctgt ctcaggttgt ctgctaaggt
    17221 tttttagctg actatctgca ctcacagatg gaaaattagc cactgttatt tgaggaagag
    17281 tggtgattcc agcttgttca gactgccatt tgtacgcaaa agacggatac atgttagtaa
    17341 gtacatcgtc caccaagcga taatcaaaat aatattgccc tgaagccaat gcaggtagtg
    17401 tagaagtagc tgtatttaca cccccgttat ttgattggat cgtttggaaa agttgatctg
    17461 tccatcctgt cgctaatttt tgggttcctt tcgaataaag cctgtaatgg atcgaaccct
    17521 tttttagttc agttgctgac caattggccg tgacttggta cgatgcagaa gaaaggagct
    17581 ttccccactg actatcggct gttaagtttg ttggtaaaac cgttccgtct agcttgtact
    17641 gctccaatac ctttaaagca acaggagaaa gcaatccgcc ttgcgcatct ttcccactgt
    17701 acacgatatt ggctgtctga ttcaatccac ttgtgcttgc tggagtatga gtcagtattg
    17761 ctccttgtgt aacgtttttt cctgtaaaat agttgactac gcgatcaaag cgactaaatg
    17821 gcaactgatc aaaacgtcct tcagtcagcc ccatcgtcaa tgtccgacta ttggtagagc
    17881 ctggaaggtt ctggttattt ccatccacgt attctgtggt atcagcaaat gtattatatt
    17941 ttcctgttga atcacttgtt gagatgtaac tattccacgt atttatctct gcacttgcag
    18001 aaaatgcata tgctcctgga ttcaagtttc cgccagttaa tttaggataa aatctacctg
    18061 cagctggagc taaaatcgaa ttgaacatac gatcttggat aatattattc gaatcataaa
    18121 aatcaacaga aaaaacggcc tttttagaca tagaaggttg taatttaccc ttggtcaaaa
    18181 ttttgtttgg actcgtatca tatttatctc cgttttctgt catcttcgcg acattagctg
    18241 taacatcaaa ttgaagcggg atatcgtcca cgttgaattc gcctcgcgta ataatagcaa
    18301 taatatctgc caatagagta tttgtatccc acgaccccat cccaaaacgc aaggtatggc
    18361 ttcctgctga taataaacta ccgctagtaa ccttgatggg ggcaccggca atatacaagt
    18421 aggcgttgtt ccagtcaatt gcttttaata catcactgct tgatacatgt tccggcaaat
    18481 tgatgatcgc cacaaatgca aatccttgtg cagtgatatt caccaaatca agtacatttg
    18541 ttagtttcac atggctttcc caatgaaaac ggaccgtgcc acctttccaa ggatcatttg
    18601 aaccatcgtt caaataagta gcagtcaatt taggcaattg gttagcccca gttagttgga
    18661 caagcttccc gccattgact gcactacgtc caccatttgg aggtacgata ttttcatacg
    18721 tgtatggatc ataagaaatg gcaggaggcg ttgctccaac agcttctgcc gaaggtgaaa
    18781 agataaatat acttaaaaca aaaagcgaag taaaaaagaa ggagcgaaaa aatgaaaatt
    18841 tattttgtag caacatgttc atcctccttt tcctttcttc tccataacaa caaccacgca
    18901 agaagccata attctatacc aatgatcaaa taaagaaaac ggatcttttc ttcattcgtc
    18961 tccgggaata cgccatgttg attagattct ttataaatcg gtagctgtgt ctgctgtttt
    19021 gatgcgccat tgactgaacc acttggatgc ggaactggac ttagatcata aaatgtaatc
    19081 gtacctgaac ttttggtatc tgcttggact ggctcttgcc agctgaagaa cagcaaactt
    19141 gcaataatcc ctcccaataa gatcgatgtt ttcaacgaaa tcacctcatc atgttgtttc
    19201 tccttttttc tttttcttgt ggacccacaa aaacagcaaa atcaaccata acactagaac
    19261 tgcagcaacc aatcctgcat aaagccatgg gaaagattgt tcttgtactg caaaaacgga
    19321 attttcattt actttttgtg ctttttcttt tttgatcgta aagttttgtg taaatcgcca
    19381 ttcattactg tcgcctgatt ttgctatgat ttctgcgcgg taatcgccgg cttttgcttt
    19441 atcaccagct aaatcaagat tcaagtgaaa attcgaattt ggtgcaacta aaaattcatt
    19501 agacgtattt tcaatataaa gctgattttc ataaaagatc ttgctcgtaa atatcaaatt
    19561 gctgataatc ctaggctgag gattttggaa acgaagctga acagttggct ttgcgttgac
    19621 ctcgtccaaa tctcctgata aaagtttcag atccggttct atcgtctctt gactttcttg
    19681 taaaacgact gcgagattat aagcaatacg atttttgatt tgtgctcctt ctgtagcatt
    19741 ttctgtttca gcttcttctt taggtgaaac atgaataccc gctaatacac gtcccttaaa
    19801 gctttttgct gggattttca cttctatagg tatagttgtt tctgcatgtg ctgatacatt
    19861 gatttccttt ttcgcaatga gcaaaacgtc acggacatca aacggcaatg tctcatctaa
    19921 cgttgggttt ttttgactat aatcgatcgt tccaccatca ctcgttaccg ccggattgac
    19981 agagacctca aatgttcgtt cttcatctga agaattgctg ataattactt ctagtgtctg
    20041 attttgttcc ggtgccacta ataaatcaaa ataaccgatt gctttatcta tttggttctc
    20101 tggaaatacc ggtttgattc caaagtctcc tgctgaatcc tttgcttggc tgattgacgg
    20161 aaaaatgaaa aatagactta aaatagaaaa aaacgaaaca aacagtcgtt tactttgatt
    20221 cattgtccac cacatccttt caacaacaac tgcggtaaaa aatccttcac ctatagtatt
    20281 ttcaccgcag tttgttgaat ttatttcata ctctatgcta ctgggccagc cacgaggttc
    20341 caagtcaaat cagccgtgaa tgttccagct gctgctttcg gcactttcag tgtgatattt
    20401 gctggatcat aaacagaaag ccatgttccc agaccttgat cttttgctgc tgtaaatatt
    20461 ggtgtcgctg cacttgcttt actcaaccca gttacagttt gactagttgg tccagtggat
    20521 tcgttatctg acgtactttt aacagtggat gttccaaaat ctaattcagc gccttgcaaa
    20581 acttgtgaac cgtttacaaa atctgaaacc gaaactgtca cgttccaccc ttgtgcttgg
    20641 ccatctgcac ctacacctcg acgatctgat acttggagat aaggtgtatc gttttttgaa
    20701 gcgtctactt gatacgtttt tgttccgctt tcaatctcgt gtgtaccaaa tggcaattca
    20761 ggaactacat ccaatgttaa agaacctgtc tggccagttg ctggattttc cggatctgct
    20821 ggctctactg gttttgaagg atcagtaggg ttaagaggct caacggtatt tgttggttca
    20881 gtaaaggtaa tatctccttt tgaagtcgct tgaccctcag caaaagctgg tgttgccaag
    20941 ccgcctagaa gaagtgttga aagtaataat gtcccaatca tgttcttttt cataataaat
    21001 cactcctttt tcttagtgac ctaagtatag tagagacagt atgcaaaaaa tcctcatgaa
    21061 ttcatccgct tacttgaaat tatgaccctt tttgtataaa agagaaaaaa acaataaaat
    21121 cgtttacatg aatattcttc cttttttatt aagtatgaaa aaatatttca attttatttt
    21181 ttcgtttcaa attattctat tagtgacatc cttgaactat aaaaatagga gtacggcatc
    21241 atctgccgca ctcctatttt tttcgtcatt tatttcttaa ataaaacgat tctcacgcaa
    21301 ttttattttt ttgatatctt ctactgtttg tgttccagct aattgcatga ccatttccaa
    21361 ttctgtttta aagaaatcaa acacttgatg gactcctgta cttcctccta aagacaatcc
    21421 ataaataacg ggtctgccaa tagcaacaag atcggcacct gatgcgattg ctttgaaaac
    21481 atgttgtcca cgtcgtacac cgctgtcaaa cacgattggt acacgtttat ctactgcttc
    21541 tgccacatat tgcaacgaat caaacgcagc aggcccccca tctaattgac ggccaccatg
    21601 gttagatacc caaattccac cagcaccaga ttctaatgat cgatagacat cttcttctga
    21661 ttgaacacct ttcacgtaga ctggcaaatc agaatgagca gcgataaatt ctacatcttt
    21721 tggactaagt ttttgtttgg aagatttata tacagcatcc atcgtttggc ctactcctga
    21781 ttgataagcc tgaacgatcg gcattggcag cgggaaggta aacccgtttc tgcggtctgt
    21841 ttcacggttt ccgccaactg ttgcatccgc tgtcaatacg attgcttttg ctccattacg
    21901 cttggccact tcaagaatat ccaaattgat tccattatct ttgctcatat aaaattgaaa
    21961 ccattgcggt gctttctccc cgcctgcttc tcttatctct tctaaagtac aagaagcgta
    22021 agaacttgct gtatagatcg tcccaaaatc tgctacacct tttgcagaag ctttctccgc
    22081 cttcacatga gctaatccat gagcagcaac tggtgccatg atgattggtg cagtcagcat
    22141 ttcttcgtca aaatgtgtag ttgtatcggg taattctaca tctcttaata catgaggaat
    22201 aatcaaacga tgattgaatg cccgttcatt ttcttgataa gtaaataagt cgccagcacc
    22261 actgctgatg taaccataac caccagctgg aatcacttgt gaagccgcga tttctaaatc
    22321 ttccatattg ataaaatcta ctattccttc atttgtacct gcttgatatg ttttttccat
    22381 ctcaatgcct tctctctata aaatttgaat gatgaacaat atgatagcgt ttacactttt
    22441 tatattactc ttcgacattg atacaggcaa ataatctgtt tacatttcag ccattccaga
    22501 tgtcactact ctggaagaaa tgattgtaat aaaaatagat aaaaaaccga tgggactttg
    22561 acaaaagtag tgtcaaagtc cctttttttg aataaacggt gttcaaaagc cattttctcg
    22621 gtattaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttacta
    22681 ctcgaaacta agcgactttt tcacaacctc tcgataaaac ggtgttcaat cagctgaccc
    22741 tcggtattag ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa
    22801 tactcaggtc agaacgttga cttcacagtc tcttccttaa acggtgttca aaaacttgca
    22861 tctgcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
    22921 ttattacttg atgcagggca ggtttttcac gacctctgat tcacggtgtt tctttttcat
    22981 caacattttt acgaaatact gtggaagact attgaaataa gtgtggattg tttctttgtg
    23041 tttcaatcgt ttcttgccat tcttcattta attttggatt accgttttct tccttgaact
    23101 cataaggtcc taattcaaaa atcccagaca gtatttgatt cacgatttca tatagttcag
    23161 tgtgatcaga cacatgattc ttgaagatca tctcattatc accacgtttg aacctcacat
    23221 tcgggctctc atgatgaacc tctatgcatt tttctccttt ttttaactga cggacttctg
    23281 aaaacatctc ataaatatct tctccagtaa aaccttcagc aagcgccaga ctgtccacaa
    23341 gaccgcctac tgtcagttca gagattattg cttgattgga atctaatttt aatgtatagc
    23401 ttgtcgcttc tactacgcca catgcattgc aaccgccttc tgatagttcg ctttttctaa
    23461 tcaattgaac ttctttcatt ttctcttcct cctcttaatt ttctacttat gtatttttat
    23521 cacctgacat aggtgactcc tatcaacgct atcatgtgct tttttcctca gaaatcctat
    23581 ttatccattc catctatccc ttattcttct acttttcttt ctctagcata taagagatac
    23641 gcatagtatt caagcaagga ttgtcgatat aaaaagaaca aatactgcta ttttgatttc
    23701 ttttggctat attacatata aataaaattt atcatacctg ctctttttga ctatatcatc
    23761 atctgacttc tatacttcgt ttgtattaaa acttaggagg aatcattcat gcttacaggg
    23821 ttaaacttat taaataatcc ttttctaaat aaaggaacag cttttacaaa agaagaacgg
    23881 gcaaaatatg gaatcactgg tatgcttcca agtactgtcc aaacactgga acaacaatct
    23941 gttcaagctt atggtcaata cttaagcaaa caaacagatt tggaaaaacg tatcttctta
    24001 atgaatctat tcaatacgaa tcggacattg ttttataaat tgatgggcga acatttagtt
    24061 gaattcatgc ctgtcgttta tgatcctgtc gttgcggatt caatcgaaca atacaacgaa
    24121 attttcttga aaccgcaaga tgctgcattt ctttcaatcg acgaaccaga aaacatcaaa
    24181 gcaagtttga agaacgcggc agatggacga gacattcgtt tgattgttgt gactgatgca
    24241 gaaggaatct taggtatggg cgactggggt gtgaacggtg ttgacatcgc tatcggcaaa
    24301 ttgatggtct atactgctgc tgctggtatt aatccagcac aagttttgcc tgtatcgatc
    24361 gacgctggta ctaataatga agaattattg aataatccct tatacttagg caatcgtcat
    24421 gctcgtgtag aaggcgaaac ttactatgaa ttcatcgatc aattcgttca atcagctacc
    24481 gaattattcc cagaattgtt attgcattgg gaagattttg gacgaggaaa tgctgcagcg
    24541 atcttagaaa aatacgaaga tcaaatcact actttcaacg acgatatcca aggtacagga
    24601 attgttgtcc tagcaggtgt attaggcgga ttgaatatat ccggtgaaca attgaaagat
    24661 caaataattt tgacatttgg tgctggtact gctggtgtgg gtatcgcaaa catcctttta
    24721 gatgagatga tccgtcaagg tgtacctgaa gaagaagcac gccaacattt ctatcaagta
    24781 gataaacaag gtttattgtt tgaagataca gaaggattga ctcctggtca aatcccattc
    24841 gctcgcaaac gctcagattt cactaatgct gatgaattga caaacttaga agcagttgta
    24901 aaagcgatcc atccgacaat catgattggt acttctaccc aacctggagc attcactgaa
    24961 gaaatcgtga aagaaatggc tgctcataca ccacgtccga tcatcatgcc tttgtctaat
    25021 ccaacaaaat tggcagaagc aaaagcaaaa gatttaatag aatggacgga cggaaaagca
    25081 ttagttggaa caggtatccc tgctgctgat gttgaatata atggtgtaac ttaccaaatc
    25141 ggtcaagcaa ataatgcttt gatgtaccca ggtttaggac ttggtttgat tgcttcaaca
    25201 gctacacgag taaatgctga aatcatttct caagctagtc gagcattagg cggaatcgtt
    25261 gatgtaacaa aaccaggagc agctatcttg cctccagtag caaaaatcac tgaattttct
    25321 caaacgattg ctgaaactgt cgcaaaatca gttgtcgcac aaaacttgaa tcgtgaagaa
    25381 atcacggata tcaaagaagc agttgaatct gctaaatggg ttcctgaata taaatcttta
    25441 gaagattaag atcgttatag taaaagaaca tacagaaact ttgtatgacg tctaaaaaga
    25501 aaaaagcgac tgcggcaaaa cagcggccag ccaactcctg ctcttttgtc gcttttcttt
    25561 ggcttttttc atatgataca gaataagaaa ggatctgtaa aaatggcaca aaaaacaaca
    25621 actgcaactg cagttgagga aaagaaaaaa gaaggttttt ggtccacaaa aatcaccggc
    25681 gtaagtttgc cactttattt gatcatggtc cttgtcctga tcattgcaat ggctttaggg
    25741 aaattaccga cgaatatgat tggaccaatc gcagttttgg ttattttcgg taatttattt
    25801 cattttatcg ggacaaaaat ccctatcgtc aaaagttatt tgggtggtgg ttccgttttt
    25861 gcgatcttcg cttctgctgc gattgctaca tttgggatct tacctgaata tgtagtgaaa
    25921 tccacagaaa actttgtcag caatatgggc ttttgggact tttatattgc tgccttgatt
    25981 actggtagca tccttggtat gaaccgtaat ctattgatga aagcttctgt tcgttttatt
    26041 cccgttgcac ttatttcaat ggtcgtctcc ttttttgccg taggactcgt gggcatgctt
    26101 atcggaaatg gttttgcaaa ttccgtttta tacatctcat tacctgcgat ggctggtggt
    26161 gttggagccg gagccgttcc tttatcaacg atttatgcaa atgttttagg tacggatgct
    26221 tcttctatca tttcccgcct gattcctgcg acagcaatga caaatgtctt agctatcatc
    26281 ggtgcagcat tagttgctcg tgctggacaa tcaatgccaa aattcaacgg caatggtaaa
    26341 ttaatggaaa aaggcgatct tggcgatgga aaacctcgaa ctgtgaaacc ttccattcca
    26401 cagttaggtg ttggtttgat ggtatcattg actttcttca tcttaggaca aattggtaac
    26461 tattttgttc ctaaagtcca tgcttacgca tttatgatta ttatcgttgt catctgtaaa
    26521 atcgccaacc tattaccaga gtattatgaa gatgcagcta tcatgttcaa taacttgatt
    26581 gtcaaaaact tgacagcagc tgttcttgcc ggtatcggta ttgcgttgct gaatctgaat
    26641 gtattagctt ctgctttgac atggcaattt gttgtcttat gtctgacaag tgttatcgcg
    26701 atttcagttg tttcaggatt tgtcggtcgt ttgtttggtt tatatccaat tgaatcaact
    26761 attactgctg gcttatgcaa caactcgatg ggcggaacag gaaatgtagc cgtcttatct
    26821 gcagctaatc ggatggaact tattgctttt gctcaaatgg gcaatcgact tggtggtgcc
    26881 atcgttttga tcatctctgg tttcttgatg caattacttt cttagtcaaa aaaacaaaaa
    26941 tacctgatca tcaagtatcg gcttggtgat cgggtatttt tttgataaag atttacagga
    27001 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgtgttca taaaattggt
    27061 gtgcttctac tctgtattct gcagaattaa gccttattgt cgttattcct cgttttttcg
    27121 cttcttcttc tacgccagtc atcaaagcct ttccgatgcc tcttttttca gcatatttag
    27181 acacagcaag tgccataata ttgaacattg gatcgctaaa aatcgattca taggactccg
    27241 cttgtacata gccagctgct tttttagaag taggatcttc ataaaccaag aaaaaatttt
    27301 tctctggctc cttcagcagt ttcttcattt gtctcaccgt aacggttaat ggtatatcat
    27361 accctaattg atccgcattg atttcacgta gtgcttctgc atcttctaaa cgcatttccc
    27421 gtaacatcca aatcaccagc caatttctga ttttttaaaa tgaatttttt tatcatagta
    27481 tactcttttt ttcatttaga acaatatgtt tcagtgattt tgacttgcgt tagtctagat
    27541 tctcctaatg tttgtaagat gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg
    27601 acaattactt gaaaaataaa ggtcactttt tttggagaca aaaccctgct aaatcttgct
    27661 ccccacaaac ctccaataac agcagcgggg ataacataaa acaacataga taaatcaaag
    27721 cgcataaagc tacttgttaa agcaatcgtt accaattttg ccagttgaga aaagaaaatc
    27781 gtagatagag aatatagtgt tgcttctttt attggcaatg caaacatcag catcaacaaa
    27841 gaaacattga ctggtccacc accaatacct aaaaaactag ctaaaaagcc cagaaccccc
    27901 cccacatata aggtaccaag tccatgacgt caaatgaaat tttggctgat gatgcttggt
    27961 gtagaagaac gcaaaaacca aggtcaatac cgtcaaaaaa atttggatca gctgcacatg
    28021 cttctcgttt tcaaataact gtaaaaaaac ttcgaataca atgttgccga ctactcctcc
    28081 aacaacagct ccactagata cccaaccgac aatctgccaa ttcagtgatt tcccgcttga
    28141 tacttgtcgc acagtcgaaa caatggacat cgtgaatacc gcaactgttg agtaaaaaga
    28201 aatccctgca acagaatggg cgccgataag atcaagaatc ggtttgatca acacgcctcc
    28261 acccatcccg gaaatagcgc caactgtgtt cgccagaacg atcaccacaa aataaatcag
    28321 accaatcatc ttgtatttcc tttctcttgt ttatatcttt tagcgattgt tactcttttc
    28381 ttttattata cagtaaaaga aacaaacgag gcttcgaaag cttttctact ttttaaggaa
    28441 atttagtgct aaacgaaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    28501 gccaaacaaa aatagctcct cataattttc gtttggttga acttattacc gcagatgcaa
    28561 gcttttgaac accgtttaag aaagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    28621 gaacaatttc aaaaaacagc ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag
    28681 ttggcaggag aacaccgttt aaggaagaga ctgtgaagtc aacgttttga cctgattatt
    28741 agagaaacaa atggcaaaat agttcttcat atttcgccgt ttttcgagcg aatgccgagg
    28801 ggcagctgat tgaacatcgt ttatttggaa aaagggattg tgacaagacg tttgtcacaa
    28861 tccctttttt tatatcttat taattcattc ttttg
//
LOCUS       DACDKJ010000032        27540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDKJ010000032 DACDKJ010000000
VERSION     DACDKJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.032"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            281..1759
                     /locus_tag="IV328_001557"
     CDS             281..1759
                     /locus_tag="IV328_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8937700.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2036..4096
                     /locus_tag="IV328_001558"
     CDS             2036..4096
                     /locus_tag="IV328_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937701.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4179..4748
                     /locus_tag="IV328_001559"
     CDS             4179..4748
                     /locus_tag="IV328_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8937702.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4923..5729)
                     /locus_tag="IV328_001560"
     CDS             complement(4923..5729)
                     /locus_tag="IV328_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937703.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5867..7381
                     /locus_tag="IV328_001561"
     CDS             5867..7381
                     /locus_tag="IV328_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8937704.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7784..8767)
                     /locus_tag="IV328_001562"
     CDS             complement(7784..8767)
                     /locus_tag="IV328_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8937705.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8772..9419)
                     /locus_tag="IV328_001563"
     CDS             complement(8772..9419)
                     /locus_tag="IV328_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8937706.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9504..10136)
                     /locus_tag="IV328_001564"
     CDS             complement(9504..10136)
                     /locus_tag="IV328_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8937707.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10152..10703)
                     /gene="coaC"
                     /locus_tag="IV328_001565"
     CDS             complement(10152..10703)
                     /gene="coaC"
                     /locus_tag="IV328_001565"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8937708.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10700..11437)
                     /locus_tag="IV328_001566"
     CDS             complement(10700..11437)
                     /locus_tag="IV328_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8937709.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11434..12423)
                     /locus_tag="IV328_001567"
     CDS             complement(11434..12423)
                     /locus_tag="IV328_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8937710.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12681..13223
                     /locus_tag="IV328_001568"
     CDS             12681..13223
                     /locus_tag="IV328_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8937711.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13240..14325
                     /locus_tag="IV328_001569"
     CDS             13240..14325
                     /locus_tag="IV328_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937712.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14337..15143
                     /locus_tag="IV328_001570"
     CDS             14337..15143
                     /locus_tag="IV328_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937713.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15144..15971
                     /locus_tag="IV328_001571"
     CDS             15144..15971
                     /locus_tag="IV328_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937714.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15968..17041
                     /locus_tag="IV328_001572"
     CDS             15968..17041
                     /locus_tag="IV328_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8937715.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17469..20165
                     /locus_tag="IV328_001573"
     CDS             17469..20165
                     /locus_tag="IV328_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8937716.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20242..20730
                     /locus_tag="IV328_001574"
     CDS             20242..20730
                     /locus_tag="IV328_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8937717.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            20742..21236
                     /locus_tag="IV328_001575"
     CDS             20742..21236
                     /locus_tag="IV328_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8937718.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21257..22066
                     /locus_tag="IV328_001576"
     CDS             21257..22066
                     /locus_tag="IV328_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8937719.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            22063..22884
                     /locus_tag="IV328_001577"
     CDS             22063..22884
                     /locus_tag="IV328_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8937720.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22935..25034
                     /locus_tag="IV328_001578"
     CDS             22935..25034
                     /locus_tag="IV328_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8937721.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            25031..26149
                     /locus_tag="IV328_001579"
     CDS             25031..26149
                     /locus_tag="IV328_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8937722.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26188..27066
                     /locus_tag="IV328_001580"
     CDS             26188..27066
                     /locus_tag="IV328_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8937723.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27257..27409
                     /locus_tag="IV328_001581"
     CDS             27257..27409
                     /locus_tag="IV328_001581"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937724.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVLVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8810 a         4718 c         5752 g         8260 t
ORIGIN      
        1 aataagtaaa agagcaggac ggtggccgtc ctgctctttt ttgtggttgt ttacgcaaat
       61 ggcgcaaatc ttgtacattt taacacaata aaacaaaaaa tatagaatat ttcctcataa
      121 atttttccat tttttgcaat attctcaaaa aaaagataaa aaatgagaat aactgatgaa
      181 aaaacaagca taatccatca taaaatcagt ataggtgaaa agatgaactt tcatctgcat
      241 atcaagtaat tggtatgagg cattttttga ggtgtaggat atgaagttgg attaccttat
      301 agaagaggaa gatagagaaa aactgctgct agctagatat ttagaatttg agaaaaagaa
      361 ttatgtagtg ctgcctaagt tatatgaaac attaggtttg tctaaattta aaacaagaaa
      421 ttatatcata gaattaaata aagaatttac tgaattcaaa acaaagccag agatccaaat
      481 ttcagaagaa gcagagatcc atgtcgaacg tctgaatttg acggatatca aaaatttaag
      541 aagaatctac tttcaacgat caaaaattgc tcgactgtta acggagatcg tggattcaaa
      601 agtaacgatc gaaaaatttg cagagaatca ttttttaagc atatcaagag cctatgtaaa
      661 aagaagagaa ttagtctctt ttcttgaaca gcatcaaata aagttaaaga aaaacatatt
      721 agttggggaa gaaataaaca tacggaacgt attatttggg gtatatttcg gggtctttaa
      781 tggtttttct atgccattta gagaaagcgt catgaatgaa gcaaagagta tcgtgcaata
      841 ttttgaatat ctgttcaatt taaaactgtc tgcaacaaaa cgaatcaaat tcaatttgct
      901 aaccactatt agtttgttga gggcctatgg gaatcaacca atcggaaaac gtttcttttc
      961 agatgaatac tttaagagct cagaaggaat gaagatcatc aaggatttat cacatatttc
     1021 ttttcttcat atccctaaag agcagatcat tgatgagatt tcttatatca tgttgttttt
     1081 gatcacggaa gaagaatttg aattagatgg cgcatttgga cttgcggaag ctcattttca
     1141 tgatatccac atgattagtc aaaaaatgtc agtggaattg attgatcaat taccattttc
     1201 gattgaaatc tctgaagaag aaagactata tatacgaaaa gaacttcgta tgggtatgat
     1261 gcatgtcaat cgaaaaaatt ggatattcga ctttgagatg actagcttta cgtctaaaaa
     1321 gcagctacag tttttcttcg agacgtatcc aatattcagt acagcaatcc aaaaggtcat
     1381 acaagagaat aaacgctatt tcttgggaaa agatgaacgt ttcatgattc gcttatttta
     1441 tgattatctc tttttattgg tcggtatttg tccggtatca tattttgaag caccgattca
     1501 tgtctgtatt gatttttcgc aaggatctgc ttataccgat tacattattt ctcaagtgaa
     1561 aggatttaaa aattacaata ttgtgattga gagtcgaatc acttctcgta cgaatattta
     1621 tttatctgat tgtttacttg aaaaatttaa taaagaacag ataatttgga aaaatccacc
     1681 gactccagat gactgggaat attttggaaa taccatcatc agagaaaaaa atagacattt
     1741 aactgaaaaa atcagctagt atttatggat ggcagctttg tctcacatat ttattagctg
     1801 atttatgttt ttttcaaaga aaaggtaact agatataact agatataact agatatatta
     1861 cttaacaaaa ttaaaaagta cataagttat tattccgatt ttcttgaaac aataaaaaat
     1921 caaaaaaaat actctcctaa tagtccatct tcttttgatt ttatttatat aatgaaatca
     1981 tcttatgaaa attactactt catgtttaga caagagtagg gaggactaag aataaatgtg
     2041 ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt cgtttagata aggaacggtc
     2101 gtgttctgaa aaacactacg ttaagtttat gatgcgcacg atgctgatca gtttatctgt
     2161 aggtgtctat atgcatgtac ccgttcaggc aacgacacaa accatcaagg tcgtacgtac
     2221 ctctgtttct gagaccactg attcccctat cactattact catactttca ctgatgcaaa
     2281 tggaaaacag ctacttcctg cattgacttc ctataataaa gtcagttatc ctccaacgat
     2341 aaaccactac tggttaattc ctaacaaaac attatggaca tcggaaaccg gaagcagtgt
     2401 gccgctttct atcttttccg atatgactga tcctacaaaa ggtgatcagt taaaacagat
     2461 cactgatatt ttgaatcatg ctttgacaga tataaaggac ggtggaagcg cacaagtttc
     2521 ttatgtatat gaaaaagatt attcacagtt tgaaagaaag aaaatcgatg ttctggcagg
     2581 gacaaaagtt tcgatatctg atctggtaga aaggctgaag aacgcagatg gaacacaagg
     2641 agatatgtcc aaagtaattt caaacaatgg gaatatcgtg gatacgacta atacaggaac
     2701 atctactatt tccttgtcct attttgatcc aattgcttta aaaacaatga ctgcatctgc
     2761 tgtgatccat gttcgtgctg atcaaacagc tatcaaagga aaagatcttc acttaagaac
     2821 aggagagagt tacgccccta aagatcttgt tgaatatgta acaaaaagcg atggaacttc
     2881 aggaaataaa gaagaaatca aaatgaacgg gacagtagat actacaactc cgggagaata
     2941 tcccgttgta ttaacttata tagactcgaa aacatcaaat gtggcagaaa caacagctgt
     3001 agtttatgta gaagcatctg ctgcttcatc gactgtcatc actcatttta tagatagtca
     3061 aacaaataaa gaacttcaag cagaaacaca tatgagtagt gtaaatcgta ttcctgattt
     3121 gcgcctacct gctgaatttt ctgatcgagt gccaaatata gatttatcga cttatgaaca
     3181 aaatgggaaa gtcacttcta tttctgattg gatgcaagca aacggattag acagaaaaag
     3241 tcactggtcg gagatccttg aagtacttca ctcagaactg atgaataata ggaagcaatc
     3301 aggaggggaa attaccttta cttatgttta tataccagac aatcgttcat tagaaggtca
     3361 tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa gagttgatcc aatcagcaac
     3421 aaattcctta ggtaaacccg taccgttaaa ggatgtatcg gttacaatca atgaagtacc
     3481 attggaggat ggttatttga ccaaacaaac aggtgatttt acgattatgt atagttatca
     3541 tgacccgtat tcactccaag aactccatgc tcagtcacta ttacatgttt taccattaaa
     3601 caaacagata gatcaaaaag aacaaaaaag acgagaagaa agcttgtcag aaaaaaaaca
     3661 acaaatggct catgcagttt ctccaacagg gaaagtaaaa caaacaagaa gcctagagtt
     3721 agcaagaata tctagaaaaa tctcagaatc taataaaacg ataaggcaag aagctcattt
     3781 ggccccacaa gtttctggat tggaaaatga aattttagag cctaaaataa aacaacaatc
     3841 agaaaaagaa tttgttgcta aacagtcgga agatcagaag gaatcagatg accagaagga
     3901 aacgaagaaa aaacgcttgt caactattga ttccaccgat gagcatctgc agccgcaagt
     3961 accacttcct agtccgggaa cacccgcacc tggtggaggt attccttctt ctgctttagg
     4021 agatttcatg cgaggtatgg caggaacatg ggtatacgct aatcgaaatg aatttgacgc
     4081 agatgactgg ttctgattta tatctctatc tgttatagtc aaataagtag ctgagtcata
     4141 tgttatttgg cataatggag acgaggaggg atagaaaaat gttagataaa acgttacctt
     4201 acgcagaaat ctggatgaca agaccacttc aacaaaatgt tccaatcacg cctttagcgg
     4261 aaggttttga atttcgattt tatcaaaaag aagatgaatc tgcttgggcg gagattgaga
     4321 cttccgttgg ggaatttcaa acgacagatg aagcacttga gtattttcaa caaaactttg
     4381 ctccttatcc tgaagaactt gaaagaagga tgttttttgt agagacaaaa aaaggagaaa
     4441 aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga gcaagtgtat ccggtttttc
     4501 attggttagc agtgaaacca gattaccaag gaaaagggct cgcaaaagca ttgactgcca
     4561 gagtcttgca agaatttcct accctccata cggacggacc aatctatctt cacacacaga
     4621 catggagcca tcaggcgatt gctctttata aaaaaatggg ttttactttt attgctgaaa
     4681 atctagacgg cacacctaat ccagattatg aaaaagttat gcaggtttta agtaaattag
     4741 ataaataacg aaatggtgaa ataagtcctc agtattggga taaaaaacga aatagtcatt
     4801 cctcatatct tacttttttg agttaatact atcggataac taatggcatg ccatacattc
     4861 aaaaaagaag gggcagtgac aaaagtattg tcactgcccc ttctcttgtt ttttagcatg
     4921 ttttatcgtt tgattttttt aggaataaaa ttcttgatga acgtaaattt ttggtaatcg
     4981 tttcgactat agaggtaacc aaatacactg aaaacaactg ctccaataaa attaacaaat
     5041 aaatccttca tagtatccat gattcccacg tctaagtaac cattttggat gattgtttta
     5101 tctgttttac catctgcatt ttgactttca atgattgtcc gagtgatatc tggaacatga
     5161 tagacagtgt ttccgtcttt acttaactcc acactattga tttgctggat gatcgtatct
     5221 ttttgcatat cgaattgcag taatttatct gctgaaaatt ccacaaactc ccacataaca
     5281 ccaactgtca tcgaaaaaca gaaggaaaca actgctagta ataaaggagt caagttcatg
     5341 gtttttgtat ttttattgag caaataaaca agggaaaagc cgacaccccc agctaaaaat
     5401 ccgctgaatg catgtagaaa agtatcccac attgaaaaat agccataaaa atcacttaac
     5461 tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa tgatttctag catatttgga
     5521 atctgtatct ttaatatctt ttccagaaaa taagggattt caaataaaat taatgtcagt
     5581 aataaaagca ttgtatttcc ccagtgttgg ttccagacct gatccactag cataataacg
     5641 acgatgatac gtaataaaat atagattccg tttgtgattc tacctgcagt ttttgtactg
     5701 ttcgtaaaaa aggttttcaa atgattcata aaataactcc ttcatgtata tccttaatta
     5761 tactaagaag cgtgtgttta ccaaagctat atgacttaaa gaaaaacaga gttgatattg
     5821 tgaaaaaaat cacttaatat agtataagga gaaaaggggg atacaaatga aaaaattagt
     5881 tgctggttta ttaacagccg ttgcacttct tgctatgagc gcatgcggcg cagatcagaa
     5941 aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc gaagtatcat caggcgcttc
     6001 tgaacaagta tatacagatc caaaagaaat gaaagaatct tatgatgtcg tgattattgg
     6061 agctggtggt gcagggatgg cagcagctat cgaagcaaaa gatgctgggt tgaatccagt
     6121 aattttggaa aagatgcctg ttgccggagg aaacacatct aaatcatcaa gcggtatgaa
     6181 tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa gactcgaatg atgccttcta
     6241 tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa gaaatgcttc gatactttgt
     6301 cgatcactct gctgatgcgg tcgattggct agatcaaaac ggtatcaaac tggacaactt
     6361 aacgatcact ggagggatga gtgaaaagag gacacatcgt ccgtctgatg gctcggctat
     6421 tggtggatac ttagtggagg ggttattgaa taacgtccat gagcgggaaa tccctatttt
     6481 tgtcaacgca gatgtgacag acattaagaa aaatggggaa acggtcaatg aagtgactgt
     6541 acatgttcaa ggagaagaac caaaagaggt caccggaaaa gcaatcgtag ttaccacagg
     6601 cggttttggt gccagcaaag aatttattga aaaatatcgt cccgatttag ctgattatat
     6661 tacgacaaat caagaaggat caactggaga tggtatcacc atgattgaaa aattaggtgg
     6721 acaaaccgtt gatatggata aaatacaaat tcatccaact gtgcagcagg atgagggggt
     6781 cttgattggt gaggttgttc gcggagaagg agcaatcttg gtcgatcaag aggggaaaag
     6841 atttgtcaat gaaatggata caagggataa agtatctgca gcaattacag cattgcctga
     6901 aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat cgtgccacag ctattgaatt
     6961 ttatgaccaa aaaggatatg tcacaactgg aaagacaatg gaagaattag cagaaaaaat
     7021 cgatttacca aaagaaacac tagcaaaaac agttgatgga tggaatacgg atgttgcgga
     7081 gaaaaaagat gctcaattca accgtgccac agccatggaa catgacttat cgtctccgaa
     7141 ttattatgcg atcaaaatcg caccaggaat ccattacacg atgggcggtg tgaaaatcaa
     7201 tacgaacact gaagtattag ataaagaaaa ccagccaatt agggggttat atgctgctgg
     7261 tgaagtcaca ggtggcttac atggaaacaa ccggatcggt ggaaattcag ttgcggagat
     7321 cattattttt gggcgacaag cgggcataca agcagctcga tttgcttcgg aaaatgaatg
     7381 aaatagaacg taaataaaaa gaggttgtga aatcagcgtt ttgagctaat accgagggtc
     7441 agctgattga acactgttta gtgaagaggt tgtgaaaaag ctgccctgca tcaagtaata
     7501 agaacagcca agtaaaaaca gcttcttatg tttttaactt ggttggactt attaccgcag
     7561 atgcaagctt ttgaacaccg tttattcgga aaaagagcgt gggacaaaag taaagaacac
     7621 ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagaaattc cttcggaaat
     7681 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga
     7741 gttaaacgct tctgtcccag cctctttttt gatcagcatg attttattca gttatcttca
     7801 aaatgatttt tccgatgttt ttgtttgctt ccatatattc atgcgcttgt ttgactgcgc
     7861 tgaattggaa tgtacggtca atgattggcc gaatcttgcc attttctaga aaaggcccag
     7921 ctttttgcat aaaagtttgt gcgagctctg ctttatactg atcacttcta ggtgtcaata
     7981 atgtaccaag cagttggaca cgtttcccaa tcaatacacc aaggtctatc tcaggcacga
     8041 tggtcccacc tagcatacca atcaagaccc aacgtccatc aacggctata gaagctaaat
     8101 tcttttccca gtaagaagcg ccgatgaaat ccaaaataac atcgactccc cgaccttcag
     8161 tgatttttcg tacctcttct tgaaagtctt gttttttata gttgatcaat tcatctgcac
     8221 caagttcttt acaaaaggcg agtttttctt ctgatcctgc agtaacgatg attttcgcgt
     8281 gagaaagctg tctagctaat tggatcgctg ctgttcccac accacttgct cctgcatgga
     8341 tcaagacagt ttcgccttct tgtaattttc caagccagta aagtgtttga taagcagtca
     8401 aaaaaacttc tgagattcca gcagcagaaa catagtctag cgtatcaggg agaaggattg
     8461 cacgatcggc aggcatcaca gcatattcgg cataaccacc tagattgact aatccgtata
     8521 cttttgtccc aggagtaaaa gtggagatat cacttttatt ctcaactacc tctccagcta
     8581 cttcgactcc caaaataggg tagggaggtt tcaaagagac tgattctctt ttcataatat
     8641 ctgttcgatt gacagctgct gtatggactt tgatcagtaa ttcgccttgt tttggtttag
     8701 gaacaggcac ttcaatttgt tctaatacat ctacagaacc aggatgcttg attgaaatgg
     8761 cacgcatgga tctattcctc ctttgcccat tcttggaatg ctgtattcaa ctctttgcta
     8821 ccttcagaaa gtactagagt cgtacgagaa atatccgttt cttccactag agcggcaagt
     8881 acagcagcaa ctgtagagcg agtgatcgtt tttgatgcct tctcattagg cttagttaat
     8941 tgaatggaag taacttcttc atcgttagtc aatgtgactg gttgaagaat gacaaaatct
     9001 aagttagttc gtttcagcca ttcatctgca taatgtttag taatataata atctgtcatg
     9061 gactcaggcc attttgctgg ttcatctgca taaacagcac tgaccatcag atagcgagag
     9121 acatgcgcat tttcagctgc gatcactgtt ttgattgcgc catccaagtc tacttgcaga
     9181 agattctttc cttgagagcc ggcagcaaaa atcaatacat cgatgccttt gaatgcttcg
     9241 gtcatttttt ccacggacca agttaaatca aaagagacat acgaaacatt ctcttttttt
     9301 actaatgttt gggaagcaac atctctcacc cctgcaaaaa tttcatggga acttggagct
     9361 aaatctttta tcaggtgtcg gccaatttgt ccgtttgcac ctacaacaaa aattttcatt
     9421 tggatcatcc tttcgacgtc aaatctttcc attcaatagt aacaccaaaa gagaaaaaaa
     9481 tcagagatcg gctacgatga ctatcagtta gtaagagaag ggtgcttgaa gtatcttaac
     9541 aagacattgc atactgctgc agtcgcaaga cctgcgacga ttccttcagg tataccactt
     9601 gtaaaaataa ccgctagaat agctgaataa acagcattta aatttgttcc gatgactttg
     9661 gcataatctt gctggaataa aaagtagatc atattcatca ctaaaatcgt attggtcaat
     9721 gaaccggaaa gtccagctaa gaataaagaa acggaatctg ctttttgttt cattagtttg
     9781 cgcagacctt tataaacgaa ataaggaacc accccaatca agatgcgagg aataaacgcg
     9841 atcagcaagg ctttccagct tccatgttct gttccgatga ctgggataaa cggagaaaag
     9901 acaaaagaca aaggtgtttg gataactgtg ctacggatca tgctgatcag tccaaatgta
     9961 ccgccaagaa aagcacctag tcttggtcct aaaacgatcg aagcaatgat tacaggaata
    10021 tgcatggttg ttgcattgat tggaccgata gggataaaac ctaaaggcgt aacagctaat
    10081 aaaatcaaaa tagctaaaaa catcgcagtt aatgtaaagt tttttgtatt tttcataagt
    10141 actccttttt tctaagcatt tctttcatta agagtcagca tgacctgttc gatgatcgtc
    10201 tgaatatcag ctaaggcacc ttttccaaaa tctccacaag ctaaaagaga ttctcttggt
    10261 tcaatttcct gatagccgac agatctcaat gtttccagat tacgctgagt gatcgggttt
    10321 tgatacatat tcgtgttcat ggcaggggca atcaattttg gtgttcccac tggaagcgcc
    10381 attgcgactg ttgaaagaag atcgtctgca atgccgtttg ctagcttgcc gataatgttt
    10441 gctgtagcag gcgcgatgag aaacaagtca gcttgttttg ctagttcgat atgattgatt
    10501 acctgcggat ttgattcttg catgacgtct gtatggactg cgcgcttcga cagagattgc
    10561 aaggttaatg gcgtgataaa atgtgtgctg cttttagaca taatcacatc tacctgggca
    10621 cctaatttga cgaattgact tgtaagatca gcgcttttgt aagcagaaat acttccagta
    10681 acgcctagta agatcttttt cattttgatt cctccagtct aattttctca gtaatcaatg
    10741 cagcaatctc agatttactt tgagcttctt ccactgttcc atctttagat aagagatagc
    10801 cgtggtgtgt ctcgccatgg atttccgtca aatcatttgc gaaaataaaa tcagttttgt
    10861 tatttatcag actatttttt gcgactgcca gaagagattc ctttggtaca tctactaata
    10921 gtttgaaacc aatgattttt gcttttggct gccattcacg tagataagaa atgatttttg
    10981 gtgttttctt caaaacgagc acaagatgct cggtgttaga agagatcttt gaaacagtgg
    11041 tatctgtctg ttcattttcc gcaaaccaat tgtccagttc ttcttgcgtt gaagaagtgg
    11101 gtaggttttt tgccagctgt tcttctgaaa aactaaaagc aggagtaaaa tcactgactg
    11161 ccatactgtg gataatcgca tcgtagtgtt cttttttcgt tagttcttct aattgtaaaa
    11221 ataagtccag tgcagattcg atcgtataaa tcgttatatt ggagtgtctt ttcggtttca
    11281 gtgcttgttt cgtagtcacg taatcgattg tggtatccgg attggcagct aaataatcag
    11341 ctaatgcttg ccctaatcgt cctgtcgaat gatttgtaat agcacgtact tgatcgattt
    11401 tttcagtcgt tcctccagct gtgattagaa tcttcattta tggtcaccta catttactag
    11461 tgttcttcct tggtgtgttc cagcaagtag tttagatgct gtttcaggca attgatccag
    11521 agtaatctct tggacgacca gttcatctgc aatctgcaaa tccgccatct gttgccataa
    11581 agaaagacgt tgatctattg gtacattgac agaatcaatt ccaatcaact ggatgtttcg
    11641 cagaataaaa ggcaagacag tcgcgtttag tttgattccc ccagcattgc cacataaagc
    11701 tactgcaccg ttataagaga tcaatggaag aagactgctt agctgctcgc cacctactgt
    11761 gtcgatgaca tagtcgattt gctgttttcc taaagctttt gttgtctctg gcagaaattc
    11821 atcaggtcgt acgacttgca aagcacctag actttttagc catgtgacag actcttcttt
    11881 tctggaaaag gcaatgattg actggaatcc tagtttatgt aacagggcta tcgctgtact
    11941 gcctacaccg ccagaagcac cagtaacgac aactttttta tcggaggtca taccttgacg
    12001 gattaaagcg ttcacacaca acatagcagt aaaccctgct gtaccaagga tcatcgcttg
    12061 acgcagcgtc atattttttg gtagaggaac taaccagtct cctggtactt tttgatattg
    12121 gctataaccg cctggatggg tgacgccaag cccaaaacca gtagcaagta caagatcttc
    12181 ttttgaaaaa cgtggattgt tactttggac gacagttccg gataaatcaa tccctggagt
    12241 catcggatat gttcgtatca cctgaccaga ctcgctcatt gccaatgcat ctttataatt
    12301 gacatcagaa taggcaattt ttaccaagac ttcattttca gccaactcag taaatggcag
    12361 atcactgatt gctgtatgga aaacaggttc ctttattacc tgaaaagatc gaaaagcgtt
    12421 cattcgtgag acactccttt taaaattttc ttttatcata ccataaagcc tactctttta
    12481 ttcttgttct tccgtaaatt tagacaaaaa ttaggtgaaa aataaagaat caaacaacta
    12541 gaatttattc caaaagataa agaaatattc agcaaaaaaa attttacaaa gggttgcctt
    12601 tttcaataaa tttcgtatac taatacgagt ggtagtaaac aggaagttta ctattagaaa
    12661 acttcgattc ggaggaaatt atggaaattg gtgagaagtt aagaaatttg cgcatccaaa
    12721 agaatttgac acaagaagaa ctaggcgaac gcacagattt gtcaaaaggg tatatttccc
    12781 agttggaacg agatttaagt tcaccatcaa tggaaacatt cttttctatt cttgaagtct
    12841 taggtgtgac tccagaagaa ttttttcatc aagaagcagc gaatctgcaa gtggtttatt
    12901 caaaagaaga tcatacggtt tatttcgatg aagaaaacgg gtatgaattg gaatggctcg
    12961 tggctgattc gaatgaaaaa gaaatggagc ctgtcttgct gacttttgaa ggtgcaggag
    13021 aatataagac gtttgaacct tcattatctg aaacgtttat ttttgtatta gaaggggaat
    13081 tagagttaag cttaggagag acggtctatg tggcaaaagc aggacagtcg atctattatc
    13141 aagcgacaaa acaacatcaa ttaaggaatc atctcaaaaa acgaacaaaa gtattgattg
    13201 ttgcaacgca atcatatttg taatataggg ggtaaaaacg tggggaaacc aatcatttca
    13261 tttgatcaag tagtgaaacg ctatgatgat gaaacagtat taaaaaaagt aagttttgag
    13321 atcgaacaag gaaaatttta tacactgctt ggtccttccg gctgtggtaa aacaacgatc
    13381 ctacgtatta tagctggatt caccgaagct acagaagggg acatctattt tgaagggaaa
    13441 cggttgaatg atttgccagc taataagcga caagtaaata cagtcttcca agattatgca
    13501 ctttttcctc atatgaatgt ttttgataac gttgcttttg gattgaagat caagaaaatg
    13561 tccaaatcag atatcgaaaa gaaggtaaaa gatgctttgc gtatggtcca gcttcctgga
    13621 tacgaaaaac gggagattag tgaaatgtct ggtggtcaaa gacagcgtgt agcgattgca
    13681 cgagcaattg taaatgaacc gaaagtattg ctattagatg aaccgttatc ggcgcttgat
    13741 ttgaaactac gtacagatat gcagtatgaa ctgcgggatc tgcaacaacg attaggaatc
    13801 acgtttattt ttgttactca tgaccaagaa gaagcgttgg caatgagtga tgagattttt
    13861 gtcatgaata aagggaaaat cgtccaaagt ggtacacccg tagatattta tgatgagcca
    13921 atcaatcatt tcgttgctga ttttgttggg gagagcaata tcgtggacgg tgtaatgatc
    13981 gaagataatc tagttgagtt tgttggtaag caatttgaat gtgtcgatgg cgggatgcgt
    14041 ctaaatgaac cagttgaagt tgttttacgc ccagaggatt tgacgatcac gacaccagat
    14101 aaaggaaaac tagtcgtaac ggtcgatact cagctgtttc gtggtgtcca ttatgaaatc
    14161 atctgttatg atgaacagca aaatgaatgg atggtccatt caacgaaaaa agctaaagaa
    14221 ggaagcaaag taggattggc ctttgagcca gaagatatcc atgtcatgcg tttcaacgag
    14281 tctgaagaag aattcgatgc acgtcttgac agctacgaag aatagggggg acaagaatga
    14341 aaacaatgcg caggatctat tccgtccctt atattttgtg gctcggttta ttcgtgatcg
    14401 cgcctgtatt aatgatcatt tatcaatctt tttttgatat gaatgggcaa ttcacgctga
    14461 ataattatct atcttatttt acgtcgggga catcgctttc tatgacgatc aattctgtat
    14521 ggtatgcatt tttgatcacg tttttcacgc tgatcatcag ttacccgact gcttattttt
    14581 taacaaaact gaagcacaag caattatggc tgatgctgat tattttgcct acatgggtga
    14641 atctgctgct taaagcttac gcatttattg ggattttcag tgtgcacggc agcgtgaatc
    14701 atttctttga ttttatcggg atcgggccga aacagatatt attcacagac ttcagttttc
    14761 tgttcgtggc aacgtatatc gagattccat ttatgattat gccaattttc aatgcattgg
    14821 aagagatgaa tccttctttg atcagtgcta gtagagattt aggcgcgaat agctttgaga
    14881 ccttccgacg agtgattttt ccgttatcat taaatggtgt gaaaagtggt gtccaagcag
    14941 tatttattcc ttctctttct ttattcatgc tgactcgtct gattggcgga aatcgggtga
    15001 tcactttagg aacggctatc gaacagcatt tcttggttac acaaaactgg gggatgggtt
    15061 ctacaatcgg tgtgatttta atcattgcta tgttcatcgt gatgctcctg acaggcgaga
    15121 agaaaaaagg aggtcgctca taaatgaaaa aatttaaatg gtcgtatctt tatttaggga
    15181 tcgtcttttt attcttatat gcaccgatct tctatctgat tttttattcc ttcaatgaag
    15241 gaggaacgat gaacagcttt acaggattta cttgggaaaa ttatgaagct gtttttgaag
    15301 atacacgttt gatcattatc gtattgaata cgttcatgct ggctttctta tctgcattga
    15361 tcgccacgat catcgggaca tttggggcta tgggaatcta ttacacgaaa aaacgacgtg
    15421 cacggacagc gttgttgagc atgaataaca tcttattagt ctctcctgat gttattatcg
    15481 gtgctagttt cttgattttc tttacactac taggattcgg attaggtttt cagagtgtat
    15541 tgctttccca tattgctttc agcattccaa tcgtcgtctt gatggtcttg ccaaaattgc
    15601 aggaaatgaa cgattcaatg gtcgatgcgg ctagagacct tggtgctaat aatttccaag
    15661 tagtcaaaaa tatcattttg ccttatcttt ctccaggaat catagctgga tattttatgg
    15721 catttaccta ttctcttgat gattttgcgg ttacattttt cgtcacagga aatggcttta
    15781 cgacgctctc agtagagatc tattctcgtg caagacaagg aatcagttta gaaatcaatg
    15841 cgctaagtgc gttagttttc ttgttctcca tgatattggt cattggatac tactttatta
    15901 gtaaagaaaa ccgtccaaaa cggctgaaga aaaacagaag agtacgcgag ggggtggcac
    15961 ggctgcgatg aaaaaattac aatctctgtt tttaggtatt ctcatcatta ttgcggtgct
    16021 gctgcttggt gttcgtcaac ttgaaaaaac aagtggcatg agtggagcaa aggtacttac
    16081 catctataac tggggagatt atatcgatcc tagtttgatc acgaaatttg aaaaagaata
    16141 tggatacaaa gtgaattatg aaacatttga ttctaatgaa gcgatgttca caaaaatcca
    16201 gcaaggtggg acaaactacg acatcgctat accaagtgaa tatatgatcc aaaaaatgat
    16261 caaagaaaaa ctagtacttc cattagatca ttcgaagatc aaaggattaa ataatattga
    16321 tccacgattt ttagacttgg attttgatcc agataatcag tattcgattc catatttttg
    16381 gggaacttta gggatcgtat ataacgataa attctttgat gcaaatcaaa ttaagcattg
    16441 ggatgatttg tggaagccgg aattaaaaga cagtttgatg ctgattgacg gtgcacgaga
    16501 agttatgggg ctttctttga atagtttagg ctattctttg aatagtaaga atatggcaca
    16561 gttgacagaa gcagcaaata aattgaatca tttaacacca aatgtcaaag caatcgtggc
    16621 agatgagatc aaaatgtata tgatcaatga agaagcctcc gtggcagtga cattttctgg
    16681 cgaagctgcc gatatgatgt gggaaaatga acatttacat tatgtgatcc caccagaagg
    16741 atccaatctt tggtttgata atatcgttat gccaaaaacc gtgaaaaata aagaaggtgc
    16801 ctatgatttc atcaacttta tgctagaacc ggaaaatgcg gcacaaaatg cagagtatat
    16861 tggttattct acaccaaatg aagaagcaaa agcgttattg ccaaaagaca tttcgtcgga
    16921 cgaacaattc tatccatctg atgacacgat ctcccatctt gaagtctaca aagatctagg
    16981 atcgaaatac ctaggcatct ataatgacct attcttagaa ttcaagatgt ataggagata
    17041 atggcaaaac tcagtaagaa tggggactta tcaacgttct aaagagctaa aaatcgtcct
    17101 ttttctatct gagttttcca atcttctacc aaaagcggat gaaacgccgc tttttttgtg
    17161 ttattttatt ttttctacca cgtttttcta ccagagaaaa gtggacaaaa ccattgtaaa
    17221 atagggcccg agacgggcta gtaaatctaa gattgaaaaa gttacagaag gataaagtaa
    17281 ttggaaacta tgcaaatata cttaaccctt ctgtaatcta ttaataaaac taatatatga
    17341 atccagttca atatttgtag ctatgaaaaa acataaaaat catattagat aaaaactaaa
    17401 ttggtagata aattattttg tcagatatct gaggcaaata ttcgactatt tgtgtttcaa
    17461 agtaaaaatt ggaatacaaa tatagaatag acatattgca gccgatagtt tattatgctt
    17521 ttagacacat taaactggag gaaaaaatga aaaagagcgc taaagatcga gtttttgatg
    17581 agctgatacg actaacaggg aatatgtctt ctgtcactgc cgagaaaatt tcgaagaatc
    17641 taggtatctc tcgtcagaat gccagccatt atttgacgag attagtggaa gataaaaagg
    17701 tcgagaaatt accgggaaag cctgttttgt ggaagccttt agatgaatat gcggttgtag
    17761 ataatacgga gcaaataaac gaagcctttc attctgtagt tggtcatgac ggaagtctga
    17821 gagaggtcat tcaaaaatgt atagccgctg tcaaatatcc gcctaacgga ctcagtgtat
    17881 taataaatgg agccacgggt gttggaaaaa gttttttagc cacaaaaatt tttgaatacg
    17941 ctatccatga gcaaattatt gaaaaagatg caccatttgc tatcttaaac tgtgccgatt
    18001 atgcggacaa tcccgaactg ctttcggcaa cactgtttgg atacaagaag ggggccttta
    18061 cgggggctga aaaggatacc gatggtttat tggcaacggc caacaacggc tatctttttc
    18121 tcgatgaggt ccatcgccta tcgaaagaaa atcaagaaaa gctctttcta tttattgata
    18181 ctggaaacta tcgtccagtc ggtgaaaatg taaattggca ttctgctaag gttcgtttta
    18241 ttttcgccac gacggaaaaa ggtgaaaatt atttattaga cacctttgat cggcgtattc
    18301 aaattagtgt tatgttgcca acttttgaag atcgccccat tcgagaacga ttagagctga
    18361 ttcaattatt ttttcaaaat gaagcggatg ttttacaaaa agatattatt gtttcaagag
    18421 aagccttgtc tttaatgtta tctcatccct ttagtgggaa tattggtaag ctgaagaata
    18481 tcattaaaat cagttgtgcg gatgtgtata gtcgaacaca agaagaacca ttaacgattg
    18541 ggaaaaatga gatcttgatt cagttaaata tgctggaatc cgaagtagaa agcttgccga
    18601 ttgctagttt attgataaag aaaaatcaaa agataaaaac ggatcgaatc aaaccgtttg
    18661 attatgattc gattttggaa aaaattactc agctgataaa tgaaggtaat ttcaatgaaa
    18721 atttttcttc gataaagaat gaagtacagc atttggtgca tcacattgat aaggattctt
    18781 ttttatcaat gagtagaaaa ttgatgattc agatgtttag aaaggtctgg gtatccgttt
    18841 taggaaaaac gtacggattg acgacgagtg gacctgttgc agaaattatt agcagatcct
    18901 atgtcatttc cccaagctat aagacagact taaccgggat aaatcgtaca ttagctgcaa
    18961 atctacctcg gacgacttac ttatgtaatc agttcttgga gcatttacca ccgctctcag
    19021 aaaaggacga gaactttcta agactgctta ttgtcgtgac tttatctgat tatgtagatg
    19081 aatccatcga gctgaagggc ctgttgcttg ctcacggaga tagtacggct agcagcattc
    19141 aagcagtagt gaatcaatta tgcggcaatt atgtttttga agcgttggat atgccagtag
    19201 agagtaatgt gtcagagatt gtcgaatcga caaggaattt cgtgaaacgg caagtgaata
    19261 cagatagtct agtgctgatc gtagatatgg gctctttaaa tcaaatttac acacagatca
    19321 agaacgattt aaaaggggag ttacttcttc tgaataattt gacgacttcc attgcacttg
    19381 atattggctt gaagatgacg atgaatgcgc catttaaaca gattgcggag gatgcagcag
    19441 acaagtattc aatcaatgtt caatactttg aaggcttttc tcaaagtagc aacattatca
    19501 tttcctgtat gtcgggtttg gggatttcgg acaaattgaa agatgttttt cgtcatcatt
    19561 tatcggatga atccatcgaa gttttcacga aggactacag agaactacgt gaattaatcg
    19621 accaaaatga tgaaacttat tttgacaaga caaaattggt catcacgact tcggatcttc
    19681 cacgttcctt ttcaattcct aatattaatg tttatgacat cttagacggt gatggagcag
    19741 ccaatttgga gcatattttg agtcctgaaa tgaagccggc agaatttaac cgcttgattc
    19801 aggagctagt aaaattcttt tcgttggaag gaattgcgga ccgtttgaac tttctgaacc
    19861 caacgatcgt gatgagtgaa gtggaaacag tcatttctaa atatgaaaat tactatcatt
    19921 tcactttaaa tggccgtgta aaactaaatc tctatatgca tacggcattg atgatggaac
    19981 gtttattctt ggctcggaca gaaaaatctg aagaaccgaa ttatgatttg aaaccagatg
    20041 aagatgaatt ttattatgtt tctagaagta tttttcaatc aatggagatg aaatataatt
    20101 ttaaagtcaa tgcttatgag ctttcgctgc tctatgaatt attaggaccg tatattcaaa
    20161 aataaagtgt ctaaaaaagt gtctaatctg ttttttttag gttagacact ttttttaatg
    20221 tgaattaacg tgattaaacc aatgttttta aaagttggca caggaattgc ttatataaaa
    20281 gtgaacagaa agaaaggaat taaccatatg aagaaaattc taatagcgac tcacggtcac
    20341 ttagcttcag gctttcttag cagtattcaa ttactgacgg ggaaggttca agagattacg
    20401 gtgattaatg cttatgtgga tgagtgtgat tttgaacagg agttgaatgt atttgtaaac
    20461 caggtgaacg agcaggatca gatttttgtt tttaccgatt tatttggcgg aagtgtcaat
    20521 cagaagatta ccaaaacctt tttggagaaa gagattgctg taactatttt gtctggcttt
    20581 aatctgccaa ttctgctcga aatagtattg gcaaccaagg aattaacaaa ggaggatatt
    20641 caggaactgg taaataaatg ccaacaggaa ttaaagatga ccattcttga tgaggtacat
    20701 gtagtaaatg aagaggaaga ttttttttag gaggaagaaa aatgattaaa gcagtgcgaa
    20761 ttgatgaacg attaattcat ggacaagtag cgatgacttg gacaaagagt ctaaatttaa
    20821 ccgggttagt agtagcaagc gatgaggcgg ctaccaacga tattcaaaag atgacattga
    20881 agatggctgc accatcgaat gtcaaagtca ttattaaaac agttgatgac gcgattcatt
    20941 tgttaaaaga tccgcgggcg aaggacatgc gcttgatggt tttagtccct aatgtcaaag
    21001 atgcggtaaa agttgcgaaa gctttcccaa cggaaatcga attaatgaac gtggggaatg
    21061 ctgggaaaat gacaccgcca tcggaagata agaaaacctt atcaaaggaa gtgatgttaa
    21121 cgacgtcaga aattcaagac ttaaaagaat tagttgaact ttatccagat accgtttttc
    21181 agcctacacc ggcgatggaa aaacgttcag ctatctctat tttgagtgat ttttagaaaa
    21241 aggaaggaga aaaattatgt atgatgcatt tgtagtcgct ttggccgttt ttattggaat
    21301 tgctggacat gaaatgttcg ggatgtccat gctgagtcgt ccgattgttg ttgctccgtt
    21361 agcaggagcg ttgatggggg atttgcagac ggggttgatt gtcggggcgt cactagaagc
    21421 catctttatg ggtgtcgtca atatcggaat ctctagtacg gcggagcctt ctttggctgc
    21481 tgggctggct acagcgttcg caatcaaaat gggaggaaac gtggatgcga ttattcccat
    21541 tgtctttcca ctagcagtat taggattaca gttaatgaat atgatttttt cttttgtatg
    21601 tggaccgatg gctgctaaat ttttcaaatt tgcaaaatct ggtgatcaaa aaggaatcat
    21661 caatctgcac ttctttatgt ggtttgtcca ctatgcgctt tatgctctta ttccattctt
    21721 tgccgtttta tttggagcag atgttgtcaa aagcgtcttg gataatattc cagaggtcat
    21781 catgaacgga ttaacagtcg ctggaaatct gctgccggca gttgggatgg cgatgctgtt
    21841 acggatgctt tggagtaaaa atattgctgt gtggtttttc tttggggccg ttgttatggc
    21901 gtatctcgag ttaccattga ttgcaattgc cgcagttgga acgattattg caattgttat
    21961 agcccaaaga gatctacaac taaaaaatat atctgttcat gcagttcaaa gcgatgctgc
    22021 tgtaaatgca acgcttgacc aagaagagga ggatttcttt caatgaaatt agctgatgga
    22081 atttctaaag atgagaaaaa attggttcgg aagatgtttt ggcgctcgat gactatgtat
    22141 gcttcggtga atccggtgac gatgggcggc ggtggtttcg cctattctat gatgcccttc
    22201 attaatcatt tctacaaaag tgaagaagat cgagctgcgg cgctggaacg tcatacacag
    22261 tatttttcga cgaccattcc ttgcgcgtcc tttgtcatgg ggattgcggc atcgatggag
    22321 aaggaaaatt ctgaaaaagc tggttttgac gtggaaagta tcaacgcgat caaaacgagt
    22381 ttgatgggac cattggctgg aattggcgat tcattgttct ggggagtttg gcgtgtcgtt
    22441 accgcgggac tagcgatcaa cttagccaat gccggaaata ttttggcacc aattatcttt
    22501 ttagttatgt tcaatatacc taattacttg atgcgatact atggcggcgt tttaggctat
    22561 tcattaggct ctaaatatat tgaaaagctg tatgcgaacg ggttaattga aattctgaca
    22621 aaagctgcta gtatcttggg tctgatcatg gtaggtgcaa tgacgatcag tatggtcaaa
    22681 ttcaataccg ttttgagctt ttcaatgaag ggcgaagaac taatgaatgt ccaagaaatc
    22741 ctcgatagta tttttaaagg aattgtaccg ttattggtaa cattgagctg tttcaaatta
    22801 ctagataaaa aagtcggcgt catcaaaatt atttttggaa tggtcgtctt gggaatttta
    22861 ttgtcattac ttggaatcgc atagtaggaa gctgcagcta ctaggctgca gtttttttag
    22921 caaggaggga aaagatgact attaatgaag taatttattc acccaaacac ccatttttta
    22981 atgctgagtt aaacgacgga aatgtgacta cttcaaacga atacagctac tatcggaata
    23041 ttcctgatgt aaagggattg accttcgatg taagtaatca aaagacatta gcaaaccttg
    23101 atgtttgcgg gacgattcaa acgttgacct tttatcgaga aaatttacta actgaggaaa
    23161 aaccaggtgt ttgggtcaat aaacagctct ctcaaaccaa ttcgttatcc ttaaaggttg
    23221 aagtaaacgg gattgtctac catttgtcag aatcggatca tcgtattgaa gtcgatgtga
    23281 ttcaagactg tttgcctaga tatattcatc attattcgac gtttaaggtc attgtagtat
    23341 cctttgctcc catactggct aataagcgtt taagtatgct tgttcaacag gtttacattg
    23401 tgaatgaaac acacgaaagc cttcaagttt cagtgaaaga tgtgccttcg tatcaagaga
    23461 agtattcgga tcagcaaaat gtgttgattt ctcaaaaagg aaataaggac acgatcgcac
    23521 aacaggaagt ggcaagcttc gccattgctt ttgtggaccc aaatgctttt gaagaagtaa
    23581 tgacttttca ggaatctgat atcgaaaaat ggctgaagga aacattgaac tattttgctc
    23641 agctatttgg gcagctgtca atgccaaacg atagagttgt ccatctctat cgtcgtgcac
    23701 tttatcaaag cttttctagt ttcggtatga atcgacaggg acaggtcgtc ggcagcaatt
    23761 ggggcagcta tcctgctact aatcgaattt ggaataagga tatgtattac gcatcattgc
    23821 cattcaccat gtttgaaccg gagttatgtc aaaaaacggt tctatggttt gatcaatatg
    23881 gcgttaagtt tcctggttca aaatttacag gaggtatcaa tcattctctg agtaattcgt
    23941 tatccagtgc gttactggct agtctttatt ttgaacatac tggggataca aaattctttg
    24001 agcggtgtcc gcaggttcta ctgaatgctt caagaattat tgaggacata ttagctcaac
    24061 gtcggtcagg ggaaccaatg ctttttccat ctgtttggtt atcagacgcc tttgcacttg
    24121 gaaagtatca taccggcagc aatctttgtc tttggaaggc ctgtattgga cttgcagaaa
    24181 tttttgaagc cctagaacaa ctgagtctta gcaagcgata caaaaacatt gcctgtaaac
    24241 taaaggaatc aatttgtaaa caaatgacta tcgatggtcc ttttggtgag caatttttgg
    24301 agggaatcgg tgatgaggag aaaacaacct attcagtgaa gcactatcaa aaaccgattc
    24361 ttgagcaagg gttaatcttt ttaagtgatg tgattgtgga tggaaaaatt aatttattga
    24421 tgcatgatgg agaagaatca gacactacgt tgatgccttt ttatcaattt ttagataata
    24481 aagatcattt atatcagaac accatggcct tttctgctag ttctttcaat ccaacctata
    24541 gcgaggaaat caaaggcatc acttggggat tagaatccgg cgccactttt cctggcttta
    24601 tcactgtttt aatgagcgag ctgcacaacc ctcaagcttt cgtaacccga ttagaagaac
    24661 taatttgttt ggcagattta gacggttcgt ggtggtggtg gccgtatcgc ttagaggctc
    24721 aacaaggaga agtcgtgcga gactttggct gcggcaaatg cggctgggca tcgggtcttt
    24781 ttgtcagcct aatgatcagt caatatttcg gattgaaatt caaaaaagga gtattgcaaa
    24841 ttgatcctgt tgataattta acgttttctt ggaagaattt gaagttcggt caagccttca
    24901 tttcgattga atgtacgcaa tccgagttga gtatttcaaa tttgtcagaa aagccattga
    24961 aaataagtga tacgaaaaaa attcttcacg tagaaaaggg aaaaacgatt cacattcaaa
    25021 ggagaaaaag atgagtatac taaaaaatat tacccaattt aaaaaaatcc agagtcgagc
    25081 aatcactccc gaaaatccaa caggcgagaa aggtaaagct gcgatgttgc caagcgagct
    25141 aggaccaagc cgcaaaggtc gtggcgcaat tccgctgact tctggtgaag aaactgtgat
    25201 tgcggatatt actggaacgg gaataattcg tcatatgtgg atgacgattc gtgatcgaac
    25261 agaatttggc agttttgttt tacgtgattt aatcttaaga atttattggg atgggtcgga
    25321 tagccctgct gttgaaagtc ctttagggga tttcttttgt aatgggtttg gagagcgttg
    25381 cgaggtgaac tcgttgccga tagtggttaa tcctacaggg ggctttaatt cctatttcga
    25441 aatgcctttc caaacatccg ctaaaattac gatcgagaat ttacatccga aggatatgtc
    25501 ctcatttttt tatacgataa attacgcttt ggtggacgaa ttggaagaaa atagcctata
    25561 ttttcacgca aattggcatc gggaacgcat taccaaatta caggaggatt ataagttaat
    25621 tgatcgatta aagggtcaag gctattacgt gggtacttac cttgcgctta cctgcctaga
    25681 acgttattgg tggggcgaag gcgagtttaa attctacatt gatggggata gtgagtatcc
    25741 gaccgtaacc tcaacaggtt cagaagatta ttttggcggg gcgtgggcct ttcatgagta
    25801 cgatgagttc ggacgtccac atgcgaaaaa ctattcaaca gcctttttag ggtatcctta
    25861 ccaatcaaat agagatgcca caagagatcg ttttgaaacg ggaaaattga atgccgttca
    25921 tgcctttggt gacgacgcaa tgccacgaca tggtctttat cgttggcatc ttcaagaccc
    25981 catctgtttt accgaagatt tatcggtcac tttacaacaa atcggcaacg atgatgttcg
    26041 cttatttgag cggcaagacg atgttgctag tgtcgcttac tggtattcaa cagaagcttc
    26101 aggaaatact aaacgtcatt tcactgatcg agaacgcttg ccgcgttaat gaagtgtttt
    26161 tcacatattc gaagggagga acctatgatg atatatgggg caattgaagc tggagggact
    26221 aaatttgtct gtgcagtcgg tactgaggaa ttaacgattt tggaaagagt cagttttccc
    26281 acaacaactc cagttgaaac aatgaagcag gtcattgatt tttttaaacc ttttaaggaa
    26341 caattggcag gcatcggggt cggctgcttt ggtccaattg atattcaatg caaatcaacg
    26401 agtttcggtc atatcacatc aacaccaaaa cttgcttggc aaaatttcaa ttttgtagga
    26461 acgctaaagc aatatttcaa tattccgatt gaatggacaa cagatgtcaa tgcggcttgc
    26521 tatggtgaat atgttgctgg ttctggcaaa gggttagcag gtgttgcata cttcactgtg
    26581 ggaacaggta tcggaggtgg tgccttgttc aatgggactt tcgtggaagg gttcagtcat
    26641 ccggaaatgg ggcatatgtt gatcaagcga catccaaagg acaatttttc aggaaactgt
    26701 cccttccacc aagattgttt agaggggatg gcagcaggac cagcgattga aaaacggttg
    26761 ggtgtaaaag gccagaattt attggctgac gattcgtttt ggcagattga agccttttac
    26821 cttgcccaat gtgcatacaa tacaacactg atgttttcgc cagatagaat tatttttggc
    26881 ggtggtgtaa tgaaacaaga acacatgaag aaaaaagttc aggataaatt tgttgagctt
    26941 ataaatggat atgttgaaat accaccaatt gatagctaca ttattacgcc cgaattaggt
    27001 gataatgccg gaataatcgg cggattagca ttggccagaa aagcggtaag aaataagcaa
    27061 ccataattaa tgaatgtttc attgatactc atgtcaaaag tatttaaccg agtaatcaaa
    27121 tgatcaaatg aagttggata aagcgaaaat gaattcaaat aaaaaggctc taagtcaaat
    27181 cgtgttgttt tcattaaatc actgaaagga aatgcttctg cgtttccttt tttctttttg
    27241 ttgtgcgctg tataccttga ggatattcac tggtggcgta cacttaaata gtaatatatt
    27301 gtgttacttt atgtctgtgg tattagttat agttgcttac caatgtatgt tcatttttcg
    27361 aaatgttgac ttcataatcc ttggcttaat gtttgaattt ttttggtgat agattttttc
    27421 agccgtataa aataaatcgt aattctatta tagagagttg tgattataaa aagatagcaa
    27481 gaaaaaaaaa gaaagtttct atacatttta aatattttat tcctatttct attgctaaaa
//
LOCUS       DACDKJ010000033        25607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDKJ010000033 DACDKJ010000000
VERSION     DACDKJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.033"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            70..624
                     /locus_tag="IV328_001582"
     CDS             70..624
                     /locus_tag="IV328_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8937725.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(797..1270)
                     /locus_tag="IV328_001583"
     CDS             complement(797..1270)
                     /locus_tag="IV328_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8937726.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1282..1818
                     /locus_tag="IV328_001584"
     CDS             1282..1818
                     /locus_tag="IV328_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8937727.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1858..2229
                     /locus_tag="IV328_001585"
     CDS             1858..2229
                     /locus_tag="IV328_001585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8937728.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2513..3784
                     /locus_tag="IV328_001586"
     CDS             2513..3784
                     /locus_tag="IV328_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8937729.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4029..5054
                     /locus_tag="IV328_001587"
     CDS             4029..5054
                     /locus_tag="IV328_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8937730.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5348..6457
                     /locus_tag="IV328_001588"
     CDS             5348..6457
                     /locus_tag="IV328_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8937731.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6540..7436
                     /locus_tag="IV328_001589"
                     /pseudo
     CDS             6540..7436
                     /locus_tag="IV328_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7452..8276
                     /locus_tag="IV328_001590"
     CDS             7452..8276
                     /locus_tag="IV328_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8937732.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8308..9618
                     /locus_tag="IV328_001591"
     CDS             8308..9618
                     /locus_tag="IV328_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8937733.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9766..11025
                     /locus_tag="IV328_001592"
     CDS             9766..11025
                     /locus_tag="IV328_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8937734.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEYRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11035..11907
                     /locus_tag="IV328_001593"
     CDS             11035..11907
                     /locus_tag="IV328_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8937735.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11919..12749
                     /locus_tag="IV328_001594"
     CDS             11919..12749
                     /locus_tag="IV328_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8937736.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12789..14972
                     /locus_tag="IV328_001595"
     CDS             12789..14972
                     /locus_tag="IV328_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8937737.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15033..16253
                     /locus_tag="IV328_001596"
     CDS             15033..16253
                     /locus_tag="IV328_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8937738.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16250..17710
                     /gene="gtfA"
                     /locus_tag="IV328_001597"
     CDS             16250..17710
                     /gene="gtfA"
                     /locus_tag="IV328_001597"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8937739.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17787..18893
                     /locus_tag="IV328_001598"
                     /pseudo
     CDS             17787..18893
                     /locus_tag="IV328_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19215..19424
                     /locus_tag="IV328_001599"
     CDS             19215..19424
                     /locus_tag="IV328_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8937740.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19417..19566
                     /locus_tag="IV328_001600"
     CDS             19417..19566
                     /locus_tag="IV328_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP8937741.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19601..20385
                     /locus_tag="IV328_001601"
                     /pseudo
     CDS             19601..20385
                     /locus_tag="IV328_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20669..21754)
                     /gene="xerS"
                     /locus_tag="IV328_001602"
     CDS             complement(20669..21754)
                     /gene="xerS"
                     /locus_tag="IV328_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8937742.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22088..22321
                     /locus_tag="IV328_001603"
     CDS             22088..22321
                     /locus_tag="IV328_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8937743.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22318..22725
                     /locus_tag="IV328_001604"
     CDS             22318..22725
                     /locus_tag="IV328_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8937744.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23862..24380)
                     /locus_tag="IV328_001605"
     CDS             complement(23862..24380)
                     /locus_tag="IV328_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8937745.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25498..>25607
                     /locus_tag="IV328_001606"
     CDS             25498..>25607
                     /locus_tag="IV328_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8937746.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         8724 a         3917 c         4783 g         8183 t
ORIGIN      
        1 aagttaatga ttatggtaaa atatatacat aatcattaac tcagaatatt tttaagaagg
       61 gaaacaatag tgagctataa tgtacaaccg ttaaggacaa atgaagagat acaagatttt
      121 cagttttggc taagaagaac aagtaatccg gaaagagata gttttttatt tttgtttgga
      181 atcaataatg ggttacgtat gtcagatatt ataggattaa aagttggaga tatacgaggc
      241 aaaagtaagc ctattattgt tgaacataaa acaggtaagc gaaaaccgat ttttatagat
      301 aatttgagag aagaaatttt attatatact gaaggtaaag aagaaaatga ttggttattt
      361 ccgagtcggc aacaaggtcg acatataaca agggatcgtg tctatcagat atatgcagat
      421 atagcagaaa aattaggacg agatgatata ggaacacata cattaagaaa aacttttggg
      481 tatcactact acaagaaaac tagagacatt gctaccttaa tgtttatttt taatcatagc
      541 tcgcaagcaa ttacaaaaag gtacattggt ataactgaag atgaaatagg agcatcactt
      601 cgtggattta aactaggtgt ataatttaaa aatttttata aacagtaaag acaaagatta
      661 aaagctattt ttatctagaa cgattttatt tcataaagtt cgcggaataa aagctggggt
      721 tactggtact tggtataggt tcgtgttata taaactaagt aattaaataa catttttagg
      781 ttggaaaagg atgaaattaa aaaaagagaa tcaatacggt ctcaaaaacg caaacgctgc
      841 ggaataagaa aaatactgat aactcaacgt ttagcagact atctaatagg gatagtctgc
      901 ttttttcgtt tttgaccata cttttgacct tctgcaaact tccttgcttt tcctctgtag
      961 aactcatgaa atctgcaaaa cgaattgccg tttcctctgt ctgctttttc gcgatatggg
     1021 tgtatatatc atccatagtg gttttaatat ctgtatgtcc aagtcgaaca tacacttctt
     1081 tgacagacgc accagattca aagagcagac tacaatgggt atgacgaaaa ccatgaagtg
     1141 tgatatgttt taagttattc tctttaatga tgattttcaa attgtgattt acatagtcta
     1201 aataaagggg cttattttct tctgatgaaa acaaaagttg ttcaccatct tttataggtt
     1261 tgattctcat ttttagatat tatgcccgca cgtattcatc acatttctat tgtgaatcgc
     1321 tttattcgac cgacatttga cttttatcat aatatactcg gtttaaaatt actaatgaaa
     1381 acaattaatc aagacgatca taccatgtat cacctgtttt tttcagataa tcaccaccgt
     1441 gtgggaactg aactgacttt ttttgaagta caagaaggga acaaccagtt ttttggaacc
     1501 aatacgattg aacgaacgat actaaaagtt ccttccgaag catcattgca tttttgggaa
     1561 atatattttg aaacacaagg cgtttgccat tatggaatcg aatcattttc tggacgacct
     1621 atcttacgtt ttgaagggcc agatgcaaca cgattagctt tagttccctt acgcgaattt
     1681 gaagatattg atgattattt cccgtatgtc acagaccaga tccctacaga gcatgccatt
     1741 ttaggaattg atgccattca actacgcgta caatattccg atgcgactga acgagagtta
     1801 ttgtcggtag gctggtagca caaagaatac gtcagtttct tttcgggtca tcaacgagtg
     1861 acaatcttag aaaatcaaga cactcagttc tatcaagaag ttcagattat tcaagatcgt
     1921 gagaatccag ttgccattga agggattggt agcgtccatc atgttgcgtt tggcgtagaa
     1981 aataaatctg atttacaacg catcgacaaa caattacaag agcgcaattt tataaattca
     2041 ggaataaaag atcgtgaatt ttttgtatca ctttattatc gtgacccaaa tcaattattg
     2101 attgaaattg ctacagggga aggaaaccta gatgcaaaag cgtatgaaaa tcaaagtcca
     2161 cgctttgaag aaattccttt atttttaccg cagtacttaa atttcattcg tgaacaagtg
     2221 gagcaataat tagctaatca aatccatgac gaagagtaag aggttgactc ataagtcaaa
     2281 aaataaagaa aaacgcatca aatccattct aaagaatgtt gatttgatgc ggttcctatt
     2341 tttataaact atcattttcg actaataaaa ctacttatga gtcagcctct ctttttttct
     2401 tgatttttat acctctttct aggtaaacta aacatctgcc aaaaataccg tgtctcattg
     2461 aaacattctc aactatttca aaataaaggg gagtaccaaa tgaacgagta tattgaaatt
     2521 atccacgcaa gagaaacaac ttaaaaatgt cagtctcaaa attcccaaaa ataaaattag
     2581 tatttttacc agcgtatctg gttcaggaaa atcatcgatt gtttttgata ctattaccca
     2641 agaagctggt cgtcaactga atgacaccta tgatagtttt acccgtttat ttttacctaa
     2701 atacagtcgt ccagaggttg atgagattga taatctttct acggcgattg tggtcaatca
     2761 aaagaagatt ggtggcagtg cgcgctcaac gttagggacg attactaaca tcaatgcgtt
     2821 atttcgtgtg ttgttttcat gttttgcaac acctagttta ggatatgcaa atgcttattc
     2881 atttaatgat cctagtggaa tgtgttctgt ctgtcaggga atcggtaaga cgattgcatt
     2941 tttaatagga ttgctttatt tttcggctat tgataatgct gattttgtat tttctgatcc
     3001 atttaccgca gaagttttgt ctggaattga ggaacaggca aagaatcatg gttattttgt
     3061 attggtgcat aatgttaatt cagcagaaga tgttaaaact attcagagaa attggcgttt
     3121 tgaggggttc attgttgttg gtgtacgtgt aaaagaattt ttggaattaa ataacgtttt
     3181 tgatggtcct gttgtttata ttgatacgca tctttcaaaa gagacattaa gagaacttga
     3241 acaacaaatg aatcgtttat ttgtaaatac aaatgattac caagctagta cattggcagt
     3301 ggaacatttg attcattatg gtcatgagaa aatagcgttt ctttcttttg actacattgt
     3361 aggtgaacca agtgttattc aagaacgaca tcaagcttat atcgatacat taaagaaata
     3421 tcatttaacc tttaaatcgc attggatata taacaactct gaatttgagc gaatttatca
     3481 agaattagct gaatttactg caattgttgt tactggagat tatttagcgg ctaaattagt
     3541 aaaattttta aaaaataaga ataaacgagt gatggaacaa ttatcgatta ttggatttga
     3601 tgatattact tttgcagaat taatggatcc tgcgttgaca acaatacgac taaatccagg
     3661 taacaagggg gcgattgctt ttcaccaact gattggactg gccaaacaag aagaaataaa
     3721 aaatcaagta ctactattag agggagaatt gatttctcga gactctgttc acaaaatcaa
     3781 ataaaaaagc tctgtgaaat aagtatagtt aacaataaaa tatccgaacg atagagagaa
     3841 tgaactgtga tctagcagtt cctcgtcgca aattcactag aattagcatt atttactcta
     3901 aattgttcgg atattttttc ttgtttttaa ttatgattac agtgcctatt tttaacgaaa
     3961 tttgcttatt ttcaaaatgt atgattatat gttagtaaac actttcaaat atgaaaagag
     4021 gagctattat ggtaggaatt agggatgttg caaaacgggc gggtgtgtcc attgcaacag
     4081 tatcgcgcgt gctaaatgag gatccgacac tttcagtaac cgatgaaacc aaacgaaaga
     4141 ttgcagaatc ggttaatttt tatcattatg tcaaaaaaag tcccgttgca aaaaatttaa
     4201 caaatattgc attaataacg acagtttctg aagtagatga gttggaagat ccctattttc
     4261 gaacgattcg cagagggatt caagtcgaag cagaacaaag caaagtcaat ttgaagaaaa
     4321 ttattcgttt gtcagaaaca ccgttggagc aagacgattt gctaaaatgt caaggtgtgt
     4381 tgattattgg gcaggtgcta tcttctgtga ttgaagaagt atcagcaatt aattcgaatg
     4441 tcgtagtgat tgatgaccct agtattgaag ctgaagtaga tgcagtttat actgatttaa
     4501 caaaagcaac gaatcagcat ttagatcgat tgtataaaaa aggacaccga aacattgcgt
     4561 ttatcggagg aggacgtgtt cgtttagata gccaaggaaa taaacacgtg tcagatgatg
     4621 attatcgaaa aatagcctat gagacgtgga tgaaacaaaa aggattagaa aaatatagtc
     4681 aaatttattt agaaggctgg accacacttg acggaatgca ggcagcagat ttacttttga
     4741 aaaaacatgc taaagataag ttgccgactg ctatcatggt aggaaatgat cctattgctg
     4801 tgggggttta tcgttcactt caaaaaaatg gtctacatat tccagaagat atttcgattg
     4861 tgagttttga taatattgaa gttgctgaat ttttaacacc tgcattaaat acggtgaatg
     4921 tgaatacaga agaaattgga aaactagcag ttcgcatggt taatgaacga ataaaagaag
     4981 ttagaaatgt ttcgatcaaa gtgattgttt ctaataatat tattgttaga gaaagtgaaa
     5041 aaaatatcga ttaaaaaagt aaacatccaa aaaagggtgt ttactttttt gtttactaat
     5101 atttttagta aaaaatctcg acaaaacatt aagtaaacag ttatggtgtt gttaacaaaa
     5161 agaaaacgca ttcaaaacag gaggaattaa gatgaaacat ttcaaaaaaa tagctttaac
     5221 agggatagct gttttaactg ggacattact cgcagcatgt ggtgggggag atagtaattc
     5281 taaagacggg caagtaactt taagctatgc gagttgagat actgaccaag ctccaggact
     5341 aagaaaaatg ttagatgaat ttgaaaaaca aaatcctgat atcaaagtag aaatggagac
     5401 aacgccttgg gatcaatatt gggttaaatt agaagctgca acaacgggag gtaatatgcc
     5461 agatgtagta accatgcact ctagcgaatc ctataaatat atgtcacaga atgctttgat
     5521 gggacttgat gatgttatca aagaaaatga tattgacatg ggaaacttta ctgaaggaat
     5581 tgctgatttc tatacgcttg aagacgaact atacgcaatc cctaaagatg cttctgtagt
     5641 ggggctatgg tacaacaaag aattatttga taatgcaggt gtcgattatc cagatgaaac
     5701 atggacatgg gaaacactca gacaagctgc aattgattta acagatgaat caaaaggcat
     5761 ctatggtttt gcatcacata ttggaacaga gactggctac tggccattta tttatcaaaa
     5821 tgggggagaa gtatttgccg atgacaatac aaaatcaggt ttgaataccc cagaagcaca
     5881 agaagcttta gctttttatc gagatattgc acttgttgat catgcttcac caacaattac
     5941 agaattacaa gatcctgata agttaagtcg ttttcaatca ggtagattag caatgatgat
     6001 tgaaggaaac tggtttgcac cgggattttt gaaaaatgac tatctagttg aaaatgttgc
     6061 ttctgccgtg ttacctaagg gaaaagttaa tgcaacaatt acaaatggac ttggttgggc
     6121 tgccagtgcg agtacaaaat acccagaaga agtgaaaaaa ctaatggcat ttttagctag
     6181 tgaggacgcc aataaaatcc aagcggaaac gggggcatca atcccagcac taaaaggctt
     6241 tgaagacgag tgggctgctt cttcagatac aattgatttg agtgcttttg gaaaaatgtt
     6301 ggagtatgga gtccctcgtc catacaataa agatggatta cgagctgaac aagcagaagt
     6361 tcaaattatt aaccgtattc tgtctggtga agtaactgtt gaagatggaa ccgctcaaat
     6421 tgaaaaagaa gtcaatgcga tattgagcgg aaattaaatt agttaattat acaatataca
     6481 ggcgaaagga ttctatttcg tctgtatatt aatggatgaa taccaaaaag ggggatatta
     6541 tgaaaaatcg aatttttgga aaatatcgta aagatcaagt gatatggggc tatattttta
     6601 tttttccaac ggcattagga ttaattttat taaatatatg gccagcaatt caaacagcaa
     6661 ttttaagttt tcaaaaaacg gtagggttcg gtgatactga atgggtcgga ttaagaaatt
     6721 accaaaaaat gtttgcagac ggtgaagttt ttcagtcttt attaaacaca ttaatttatg
     6781 cagtggtttc gatacctagt attgttgtat tatccttgtt ggtggcagtt ttaatgaata
     6841 gaaaagttaa aggattgtcg atttatcgga caatttattt tctaccagtc gttgctgcac
     6901 catccgcagt cgcaatgatt tggcgctgga tgttcaataa tgattatgga ctgattaata
     6961 acatgttggc taaaataggt ctcgatggac cagcatggtt aacggatcca tcgattgcga
     7021 tttattctat tatcattgta ggagtttgga gtgctattgg ttataacatg gtgttattac
     7081 tagccggctt ataagaaatt cctaaagatt attatgaagc agcggaaatt gatggtgcta
     7141 gtccaatcca gcagtttttc aacattacgt taccattagt tacaccaaca ttattttttg
     7201 tagttgtaac aacagttatc aatgctttcc aagtatttga tgttattttt atgatgatta
     7261 ctcctaatag taccgccatg tttaaatcac aatcattagc ctatttattc tataaacatt
     7321 catttatttt aaatgacaag ggatatggtt cggcaatcgt tatgttcctt ttagtgatta
     7381 tattaattat tactgcaatt caaatgaagt tgcagaaaaa gtgggtaaac tattaaggag
     7441 gctatcaaat tatgaaaaat aaattttttt caaatgcgcc aatccatatc atgcttattt
     7501 taggtggtat tgcaatggca ttacctttta tttggatgat tttaacttca gggaaaacgt
     7561 tggttgaatc tacccaaatt ccacctacaa ttttccctga gaaatttcag ctaagtaact
     7621 atcaacaagt acttgattta ttaccattca tggcttttta tatcaataca tttttaatgg
     7681 ttgttggacg agtgattggc tcggttttat tttcagcaat ggctgcatat gcttgtgcgc
     7741 gtctaagttt tccagggaaa aatatccttt tcagcttagt acttattcaa atgatgattc
     7801 ccggttccat ttttattact ccacagtatt tattagcaca aaaattgggt attttaaata
     7861 caattccagc attaatatta ccaggggtag ttagtgcgtt tggtacgttt ttattaagac
     7921 aatttttctt aggtttacct gatgaattgg aggaagcggc ctatttagaa ggtgccaata
     7981 tttggacgat ctttttcaaa attatgttgc cgcttgctaa atcaggattg gttgcacttg
     8041 gaatattcac agcgttgttt gcatttaaag atttaatgtg gccattgatt gtcaatactt
     8101 caattgataa gatgccttta gctccgggat tagcaaactt acaaggacaa tatgccacga
     8161 acttcccaca attaatggca ggttcgttat tagcaatttg gccaatagtt gtgatattca
     8221 tcgttttcca aagaaaattc attgaaggaa ttgctacaac aggtagtaaa ttataaaact
     8281 attaattagt gattggagag taagtacatg acaaaaatta catttattgg tgcaggaagt
     8341 actatttttg caaaaaacgt attaggcgat gcgatgttaa ctccaagtct acaagatgca
     8401 catattgcat tgtacgatat tgatgaaaaa cgactaaaag aatcaaattt aatgcttaaa
     8461 aatattaata aaaattcgaa tgaaaatcgt gctgatattg tttcttatta tgatcgcaaa
     8521 gaagcgttaa gagaggctga ttttgttatc aatgctattc aagtgggtgg ttataaacct
     8581 tcaacagtga ttgattttga tattcctaaa aaatatgggt tgcgacaaac tattggtgac
     8641 accatgggga ttggtgggat ttttagaacc ctacggacac taccagtaat gttagacttt
     8701 gctaaagaaa tggaagaagt ttgtccaaat gcttggttat tgaactatac aaatccgatg
     8761 gcgatgttaa ccatgggtgt tttaaaagcg acgagtatta agactgttgg tttatgtcat
     8821 agcgttcaag tatgcgtacc agaattgttt gagcatttag gaatagccga tcaatatgac
     8881 ttaaatgact ttcaatggaa aattgcaggc attaatcata tggcattctt gttggaaatt
     8941 acaaaaaatg gggaagattt ctatccaaca attcgtcagt tagctgcaga aaaaccaaat
     9001 ccacatccag attctgtacg ttttgaattg ttgaaacgtt ttggttacta tattacagaa
     9061 tcaagtgaac acaatgcaga ataccatcca tactttatta aatcaaaata cccagaattg
     9121 attgacgaat tagggattcc gttggatgaa tacttacgtc gttgtgaaca acaaattaat
     9181 gattgggaaa gcatgcgaga ggacattgta aataatgggg agttaacaca tacgagaagc
     9241 cgtgaatatg cttcatatat catggattct attacgacag gaactccgtc agtgattgca
     9301 ggaaatgtct taaataaagg cttaatcaca aacttaccga cagatgtttg tgtggaagta
     9361 ccttgtttag tcgataaaaa tggcgtgcaa ccaacctatg tcggtgaatt gccaacacaa
     9421 ttagctgctt taaatcggac gaatatcaat gttcaagaac tgacagtaga agccgcttta
     9481 acactagata aatcaaaaat ttatcaagca gcgtatttag atccacatct atcatctgaa
     9541 ttgtctttaa cagatatcaa aaatttagtg gatgatttaa tcgaagcaca tggggactat
     9601 ttacctgaat ataattaaaa agaagctgaa agagtaagga attttcaatt ccttactctt
     9661 ttcttagtta aatttagtaa aaatattgac aaaacactta gtaaacagtt acattagtgt
     9721 taacaaaaag aaaacgcatt caaattgtat aaggaggagc ataaaatgaa gtggtctaaa
     9781 aaaattattt tagcttgtat agcactagga gcaacgatct ctttgactgc ttgtggaaaa
     9841 aatcaggaag atgcaagcgg taaggtagaa atagagtttt ttaatcaaaa aaaagaaatg
     9901 actcaaacga ttcaggagat tgctaaagat tttgaagcaa aaaaccctga tattcatgta
     9961 aaagttgtgg atgtaccgaa tgctggtgaa gtaattaaaa cacgtatgct tgcaggcgat
    10021 gtaccagatg tcattaatct ttatcctcaa agtatagaat tgaaagaatg ggcgaaagct
    10081 ggttatttag aggacttaac cgaagaaccg tatttagaga atatcaaaaa tggatatgca
    10141 caacgttttg ctattgagta tcgtgtatat agcataccat taaccgctaa tgtctatggt
    10201 ttttactata acaaaactgc ttttgaggaa atggggattc aagcaccaga aacttgggca
    10261 gaatttgaaa aaatagtagc tgagattaaa gatcaaaaca aagtaccgtt tgcgattgca
    10321 ggagcagaag gctggacttt aaatggttat caccaactag ctatagcgac tgctgctggt
    10381 ggtgaagagg aagcgaacaa tgtttggcgt ttctcagaag ttaatggaat taatgctgaa
    10441 agcaaagaaa tgcaaaaaga ttttaatcga ttagatttgt taagagaacc aaaagctttg
    10501 caaaacaact ggcaaggtgc cggttataac gatacagtag ttacttttac aaaaggtgat
    10561 gcgttgatta tgccgaatgg ttcatgggca atgccgatga ttcattcaca aaatccagat
    10621 tttgaagtag gaacttttcc ttttccagca gatgaagcag gtcaaagtct aacgattggc
    10681 gcaggagacc tagccttatc agtttctgca acttctcaac ataaagaaga agccaaacgt
    10741 tttgtagaat atatgacaac acctgaagcg atgcagaaat attatgatgt agatggtgca
    10801 ccgtgtgctg ttgagggcgt tatcgagaat actcaggatt caccattaag tggtttaact
    10861 gaacttgctt ttactgatcg tcatttagtt tggttagcca aagattggaa tagtgaaaat
    10921 gatttttata ccttaacaac aaattattta cacaatggca atcaaaaagc catgattaac
    10981 gcattaaatg ctttctttaa tccaatgaaa gctgacggaa ggtaggaaaa taaaatgaaa
    11041 ataaaagaaa ttttaaatcg ttactgggca atcatttttg tagcaattcc aattattttg
    11101 cagatgattt ttttctactt tccaatggtt caaggtgctt tttacagttt aactaattgg
    11161 actggtctga cttataattt tgattttgtg ggtttgaaca attataagat tcttttttct
    11221 gatccgaagt ttatcaaatc gattggcttc actttaattg tgacgctttg catgattgct
    11281 ggacaaattg gttttggtat tttgattgcg cgggcgttga acgcaaatat taaagggaaa
    11341 acattcttta gagcatggtt cttttttcca gcggtcttat cgggcgtggt tgtttcttta
    11401 gtatttaaac aaatttttaa ctatggttta ccaaaaattg gtagtatgtt aggcattgaa
    11461 tttttacaaa ccagtctttt aggaacaaca tggggagcga tttttgcagt aatttttgtt
    11521 ttactttggc aaggaattgc catgccagtc atcattttct tagcgggttt tcaaagtatt
    11581 ccacaagaaa ttattgaagc tgctagaatg gatggggcga atgataagca aattttttgg
    11641 aatattgaaa tcacctactt attaccaagt atttcaatgg tatttattat ggcattaaaa
    11701 ggtggcttaa cagcatttga tcaaattttt gctttaactg gtggtggacc aagcgatgcc
    11761 acaacatctt tggggttact tgtttataat tatgctttta aaaacaatca gtttggttat
    11821 gcgaatgcta ttgctttagt cttatttatc ttaattggga ttgtttcact ggcacaatta
    11881 aaattatcga aaaaatttga aatctaggag gtttcccaat gaaaacatca aaagaaaaaa
    11941 atatttctaa atacttgctt ttattattag ggggcttcat gattttagtt cccttagttt
    12001 caacgctatt cggttcattt aaaacaacac ccgatattat gaataatttc tttcgttttc
    12061 ctaatccagc gactttggaa aatttccgaa gattgttagc ggatggaatt ggtcattact
    12121 tttggaattc ggcaatcatc acaatcattg cagtagttgc tgtgacagca attattccta
    12181 tggctgctta ttcactagca agaaaaatga ctcgtgggaa agcatacgga ttgatgtatt
    12241 tatttttaat tttaggaatt tttgttcctt tccaagtaat tatgattccg attaccgtta
    12301 tgatgagtaa aattggttta gcaaatatgt ggggattaat cttactctat ctcacttatg
    12361 ccgtaccaca gacactattt ttatatgttg gttttattaa actaaatgtg cctatcagtc
    12421 tggatgaggc tgctgaaatt gatggggcta gtcactttac aacttatttc aaaattgttt
    12481 tcccaatgtt aaaaccaatg catgcgacga ctattattct taatgctcta tggttttgga
    12541 atgatttcat gctaccatta ttaattttaa atcgtgattc taatatgtgg acactaccat
    12601 tgtttcaata taattatacg ggacaatatt ttaacgacta tggtccaagt tttgcttcct
    12661 atacagtagg aattgtcacg attacactag tttatcttgc tttccaaaga agtattatag
    12721 ctggaatgag caatggagca gtgaaataac caataaatta ttctcattat aaaggagttc
    12781 ataaacttat gattatattt gacaatcaca cacaaacatt tcatttacaa aataaaaaaa
    12841 ttagctatct attaggaatc gaagaaaata attatttaac gcatctttat tttggaaaaa
    12901 agattacgca ctattctggt gggtatcgct acccccgaac agatcgttcc ttctcaccca
    12961 acaatgcaaa tgcgcaagat cgtttattat ctttagacac attaccatta gaatttccag
    13021 gatatggaca tggtgatttt agagaaccgg cgcataatat taaattagga aacggtagtc
    13081 gtgtaaatga ttttcgttat gatagctacg aaattcaaaa aggaaagccc agcttagcgg
    13141 gattgccagc cttatatacg gaagatgatg atgaagctga aacgctgatt attcaattga
    13201 aagacgcgat tagtggattg actttacagt tgttctacac aatttatcaa aatcacagtg
    13261 taattattcg ttctacgcaa ttagtaaata atagtgatga aacagtggag attaaccgct
    13321 tagctagtca atcaattgat tttccaaatc gtccactcga gttaattcat ttaaatggta
    13381 cctgggcaag agaacgtcaa atgactagag aaaaaattca tacagggata aaagtattag
    13441 atagtaaacg cggatcaagc agccatcaac aaaatccgtt tgttgcttta gtagatgcca
    13501 aaacagatga atttcaagga gaggcatatg gatttaattt agtttatagc ggtaatcacg
    13561 aaacggtgat tcaaaaagat ccatttgacc aaacacgggt cattatggga attaattcat
    13621 ttaattttgg ttggcaacta aaaactggcg actcatttca aacgccagaa gtcgtgatgg
    13681 tttatagtaa tgaaggtctg aatacgttat cacagacgta tcatgaattg tataatcacc
    13741 gtttaattcg tggaaactat cgtttaaaag aacgtcccac gttaatcaac aactgggaag
    13801 caacgtattt tgattttaac gaagaaaaaa tcatgaatat tgtcgatgat gcgaagaact
    13861 tgggcattga aatgtttgtt ttagatgatg gttggtttgg cggtcgtgaa aatgacttca
    13921 cttcattagg tgattgggtt gaaacagaag gcaaacttgc ttgtggtttg gaaaatttag
    13981 ccaagcaagt gcatgataac gggatgaaat ttgggatttg gtttgaaccg gaaatgattt
    14041 ctgaagatag tgaactattt cgagcacatc cagattgggc attgggtatt ccaggtcgtg
    14101 accgctcaat tagtcgaaat caatatgttc ttgatttttc aagacaagat gttcgtgata
    14161 atatctatca acaaatgacg gcgatttttg atcgtgtacc gattgattat gtgaagtggg
    14221 atatgaatcg aaatatgaca gaagtctatt ctgtcttgtt ggactcaacg atgcaaggtg
    14281 aagtggctca tcgttatatg ttagggttat atgaattttt agaaaagtta acaacgaatt
    14341 atccaactat tttatttgaa agttgttcgg gtggaggcgg tcgttttgat gcaggtttct
    14401 tgtattacat gccacaaact tggacgagcg acaataccga tgccgtggct cgtctgaaaa
    14461 ttcaatatgg aaccagctta gtgtatccaa tttcaagtat gggtgcgcat gtttcagcag
    14521 tcccaaatca tcaaacagga agagaaacga gtttaactat tcgtggcaat gctgcgatgg
    14581 ctggtgtttt tggttatgag ctagatctaa cgcaattaac ttctgaagaa aaagcagaaa
    14641 ttaaagaaca agttgccttt tataaaaagc atcgccaact tttgcaatat ggaacttttt
    14701 atcgcttgga aagtccgttt gaaggcaatg atactgcatg gatgtttgtc tcaaaagacc
    14761 aaacagaagc catggtcttt tatttccgag tattagcaga agctgcgcat ccattagtca
    14821 cattgaaatt agcaggatta gcagaagaag caacttatca aatggaagat gtaacatttg
    14881 ctggcgatga attgatgaat ctcggttttt atattgactc agaactacat ggtgattatg
    14941 cgacacaacg cttttatttg aaacagatat agtagcttga atttacaata taagtgcaac
    15001 accaaaagag actcattcca aacgaagaaa atatgtttta tttacacgca tttactaaaa
    15061 agcagcctga tttgaattgg gaaaatgaag aagtacgtga agaaatctat gcaatgatta
    15121 actattggtt agataaagga ctaggcggat ttagaatcga tgcgatatta aacatcaaaa
    15181 aaaaactaga gtatggcatc tttgaatctg atggagaaga tgggttagcc ttcattgggc
    15241 cctggattct taatcagtcg ggcattgaag tgtggttaaa agaaatgaga gagcggacat
    15301 ttaaaccaca taatagtatg acggtagcag aagccgatgt tcctaatgaa cgtttagatg
    15361 aatatattgg tgaagatggc tattattcga tggtttttga ttttagttat acggatattg
    15421 atgtacctga aactggtgaa tggtttaaag atagccaatg gacatggacg gatatgcgta
    15481 ccaatatttt tacgaatcaa ttagttacgc aagataaagg ttggggcgcc ttatatttag
    15541 aaaaccacga tcaaccacgt tcaattaata aatatattcc agaagaatgg atcaatgatt
    15601 atagtaaaaa aatgttggcg acgcttttta tgttactaag aggtacccct tttatttatc
    15661 aaggacaaga actaggaatg accaacatag aaatggcatc attagaagat tttgatgatg
    15721 tagctacgca ttctcagtat aaacgggcat tagaatatgg attggcacca gagcaagcat
    15781 tgaaagctgt caataaacga agtcgggata attctcgcac acctatgcaa tggaatacgc
    15841 aaaaaaatgc cagattctca acttctgata atgtatggtt aaaagtaaat cctaattatc
    15901 cagaactcaa tgcagaagct cagttaacca atcaacagtc ggtattcaat tattatcatc
    15961 aattaattaa attaagaaaa aatagtgtat acaaagacgt gctaatctac ggacaatttc
    16021 taccagtgaa agataaaaat gaacatattt tgctttatga acgccaatta gaagataaaa
    16081 tcgtattagt tatcttaaac gttacagcaa cagaacaaga atattatgta gatgaacagt
    16141 accaacaagt attaatcaat aattatgaag atgttttatt agatcaaagt cgaaaattgc
    16201 gtttacgacc gtttgaaagt attgtattag ctaattatgg aggacaatta tgaaaattaa
    16261 aaacgaagca atgctcatca cctatgcaga tagtttagga aaaaacatgg gcgaattaaa
    16321 acaagtctta gacaaacatt tacaaggtgt tattggtggt gtacatctgt tgcctttttt
    16381 tccatcgaca ggtgatcgag gctttgcgcc gagtgattat acgacagttg atccagctct
    16441 tggaacatgg gaagatgtgg aagcattagg tgaagattat tatttgatgt ttgattttat
    16501 gattaatcac atttctcgtg aatcaaaatt tttccaagat ttcaaaaaaa accatgagca
    16561 gtcaccatac aaagaaatgt ttatccgtat tcatgaattt ttcccagaaa atcgaccaac
    16621 acaagaagat attgatttaa tctacaagcg taaagacaaa gcgccgtttc aaacggtaga
    16681 atttgcggat ggtacgacag aagaggtttg gaataccttt ggggaagaac aaattgattt
    16741 agatgtgacc aaagaagttg tgaaagaatt tattcgagaa accatcaaag atatggcgaa
    16801 ccatggctgt tctttaattc gattagatgc gtttgcttat gcgattaaaa aattagatac
    16861 caatgatttc ttcgtggaac cagatatttg ggaactttta gacgaagtgc gtcaagaagc
    16921 agcgaaatat gacgtcgaat tattaccaga aatccatgag cattattcca ttcaaatgaa
    16981 aattgccaat catgattatt atatctatga cttcgccttg ccaatggtca ctctgtattc
    17041 actttatagt ggaaaatcag aacgtttagc caattggttg aaaatgagtc cgatgaagca
    17101 gtttacaaca ttggatacgc atgatggaat tggtgttgtg gacgctcgtg atttattaac
    17161 agatgaagaa ttggattaca cctctgagga attgtataaa gtcggggcga atgtgaagaa
    17221 ggtctattca agtgccaatt acaataattt agatatttat caaattaaca gcacctatta
    17281 tagtgcatta ggcgataatg accgtagcta tctgttagca cgtgcgattc aagtatttgc
    17341 gccagggatt ccacaaattt attacgttgg gttattagca ggaaaaaatg atattgaact
    17401 gttggaagca acgaaagaag ggcgtaatat taatcgtcat tactatgatt tagccgaaat
    17461 tgaagaagaa gtgcaacggc cagtgattca acaattattt gcacttttaa aatttagaaa
    17521 tgaatctgcg gcgtttgatt tggatggaac gattgaagta gcaacgccat cagaatcaga
    17581 aatagtgatt acacgtaaaa ataaaaacgg tagtgatagt gcaacattaa cggctaactt
    17641 ggatacgaaa gaatttatca ttgagtctaa agatataaaa attttcacta gtgttgcatt
    17701 aaaattttaa cctattccat agaacaaaaa atcctttata ctagcacttg gatgacttat
    17761 ccaagaccaa taaaaaggac tcatacatgg ataagtataa aacaaataca gcatttaata
    17821 aatggttttc tgccataaaa ttaggaaaac tttctgtcaa ctcccaaaaa tgtatttttg
    17881 attacaattc gtatcgtaaa aaactgtctt ttgaagctgt gctcaagctc tttctctacg
    17941 cagttaatga agaaaaagaa agtttgcgtg atttgagtac agctcttatc gaccgatcgc
    18001 tccaactaga aaccgatgtg tcatcgatta gtcataccca attgtctcga gcatttaatg
    18061 cacttgaacc taaagtgtta gaagaaattt ttcaacaact gttagaaaaa gttcaacatc
    18121 aaatcaaacc aaccaagcga aatcatctgt atctgattga ttccagtacg ttttcgctta
    18181 gtttaaaacg tcataagtgg gcgactttcc gtcaaacaaa atcaggtgtc aagttacact
    18241 taaactattg ttatatggac gatactacga tgtatccaac tgattttacc ttaacaaatg
    18301 cccatgaaca tgatctcaat caactaccag tattggtgaa tcaaccagag gcaacttacg
    18361 tgtttgatcg tgggtattag gactttgaaa agatggatca gatgcattgg gatggctatt
    18421 tcttcgtcac tcggattaaa aaaaacacca aagttcacgt gatagatact ctggaaacat
    18481 cgccagaaac agaaattcta cgagacgagc tggttcgttt aggatctaaa acctatctaa
    18541 ccgccaattt ccgactagtc actgttcaag ataagaacgg acgtgtgttt caatttatca
    18601 ccaatcgaat ggacgtctct tcaaaagaaa tctctgatat gtatcacgct cgttggcaaa
    18661 tcgaattgtt tttcaagcac atcaaacaac acatgaccat taaaaccttc ttttctcaaa
    18721 gtgaaaaagg cgtgcagaat caattgattt taacgatgat ttccgctctc ctcacgtttc
    18781 tcatcaaatt ggagacgaaa acggaaaaat cagtgttcca aatcaaacga ttctttcgtt
    18841 atttactgtt tcagcccttt gaatgttgtc tagaaaaatt aattcccaca tgagaaaaaa
    18901 gaaatttggc tcgttacttt tggttttcag ttgaataaca gataaaaaat tttctggaaa
    18961 aagtcatcct ctgcgtcagc acagtttttt ttgacttatt ttcagaggaa atagttttca
    19021 gaatattcta aaatgtttta tgcaacacta gtgatatttt ttagaattag atttctaatg
    19081 tgcatttttt gagtattaag gagcaagtat acaaagattt ataaagctga ttaaatttga
    19141 ataaaaatat gcaggaacgc tactattttt agtagtgttc ttttttatat ttgaataaaa
    19201 aatgtggtgg ttaaatggtg gttataacaa aaaatgaatt aatgaaatta ggttatggga
    19261 agtatcagtc tgagagaatc atacgacagg caaaagcaat tatggtttca aaaagctata
    19321 cctattacga aaatagaaga ttaggactag tacctgttga atctgtagaa gaagttttag
    19381 gtatgaagat agaggtagag gaggattttc agaatcatgg ttaattttaa aaaagataaa
    19441 aatgggaaat atcattttac tgctagtttg ggattcgaca aacgaacagg gaaaagagta
    19501 caaaaaaacg tagaggggtt aaaacagtta aagaagaaaa aaagcgtata ccgaacttgt
    19561 ttctaattat ggaaaagaag cagaaacttc tcaaagtact atgttgtttg aggagttttt
    19621 ttataaatta ttcctacctt attatcaagg gaaagtagaa gaaagaacat ataaaaatcg
    19681 gttatctaca atcaaaaaaa cttttcttat ttctttaaaa tggagttaag agaaatacgt
    19741 ccaattgaca ttcaaaagtg gcaaaataat ttattgagca aatataaaaa ttcttatgta
    19801 agaaacatgt atgggatttt tcagatgtca ttagatcgag cagttgtttt gggactactt
    19861 gaaaaaaatc ctgcaaaagt tattggaaat gtgaagaaaa ggaaaactac agttgatttt
    19921 tggacaaaag aagaatttac aaaagttatt tcaacttttt atattgatga tttgtaccaa
    19981 cattttggtt ttatttgtat gtggttactt tttatgaccg ttatgcgttt gggagaagcc
    20041 actgcattaa cttggaaaga tattgatttt gaaaataaaa acttgcgtgt gaataaaaca
    20101 ctctattata agagagccaa aacctatgag tttgtagaac ccgaaacggc ttctagtatt
    20161 cgtacaatcg aactagacga caatacaatt acctttttaa aagcttggaa ggagcgtcaa
    20221 gtttcaatag gaggcatgtc gtttgtactt tcttataatg gaacacctac tcagaaatat
    20281 acaatccgtc atattatgag aagacatgca acgatcgttg gtgttcatat aatccgcata
    20341 cacggattaa ggcatagtca tgcttctttg cttatatcac aataaaaaga tgcacttcga
    20401 attaaagaaa ggttaaaaca tgctgatgta caaacgactt taggaactta tggtcatttg
    20461 tatctaaatt cattgagaga gataacaaat gatcccacag attatatcca aactaatttt
    20521 tctaaaatca aaccgacacc tatttacgat aataaaaaat aaatgataaa caaaagtagg
    20581 atcacaccaa aagaaacaca ctagaaaaac tataatacag gcttttctag cgtgttgttt
    20641 cttatttccg cctatttata cattatactc ataaagcatt taatgcatcg atcgtagccg
    20701 cattgtctat atgggtatat aaagttgttg tattggtact tgaatgtccc aattgttggg
    20761 cagttaatac caatgaattt gtctgttggt agagtctagt agctaatgta tgacgcagtt
    20821 tgtgagggct gacttgaatt ctaaagcctt ttgaatattt tgctaccatc cgttcgattg
    20881 caataccgga tatacgcttg gcttctcctt ggtaaattgc tagaaataaa gctttttcgt
    20941 tttctgttac tttatatcgt tctttccgaa tagctagata attagctaaa tattccattg
    21001 cgaatgctgc tatattgaca gcatctcttt tatttccttt tctagttact accacattat
    21061 tcttatctaa agataggtca tctaaattaa tatttataac ctcggagagt ctcaatccag
    21121 atcctaaaat cagtgcattg atcgccaggt ctctttctac atctcgttga taatagatca
    21181 aagctcgttt actcaaagta tttttatatt cattggcaat ataatctaga taattttgtg
    21241 tatccttatc tagaaataat tttttctcaa tggcattggc acgtgcagct aacgtagttt
    21301 tatctgtgat ggttgctact tgatccatag gattattgtc taagtatggt tttcctagag
    21361 gattattgga acttttcgaa agatatttaa atagaacctt taatgccgtg atatttctat
    21421 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
    21481 ctttttttac attttctttt gtgcgacgtt tcaaatgaga tttgtacaag tttaggtcat
    21541 aggcagttaa ctgtcttatc gaggtgatag aagtatcagg aacgatagac tcactcagaa
    21601 gccattcata aaagagctta taatttttta gatattctaa agtggttcga ggtgattgat
    21661 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
    21721 tatctatttt ttgatatatt tctgtatttt tcatgtcatt catcctttca ataagtaggg
    21781 gagaagagta tctctataat tggactatac caattataga atgttttgtc aattggtatg
    21841 ctctaaaatt tattaatttg tttcaaaaaa agcatataaa caggtatacg tttaataatt
    21901 taaaataaat ggtatacata aatagggaag gaaatagaag ggagcaatct aaattttttg
    21961 aattttctta attaattata tagaaagaac aatctttcta tagagttaga catgagaggg
    22021 actatgcttg atttaaaatc aacaagcgta tatactgtat atacaaagaa aaaggaggga
    22081 gaagactatg gcaattaaag aaagaaaatt tagggaagtg gggaactcag tagtattaac
    22141 tatttcaaag gagtttctta aagaaaatgg gttaaatccc ggagatacgg tttttttaga
    22201 tgaagaaaaa cttcgagcgg caattacaaa aaaacaagcc ttatctgatg aaattgattt
    22261 agcaatatct caagcattaa ctgaatatga tactactttt agagagcttg tcgataaatg
    22321 agtattcatt acctagaagc caaagatatt ataaaaatga atgtaatcca aataaaaaaa
    22381 tactctccta atgaacccct tggtgtaaag gatgcgaatg ctttagaaat gtgtgtaggg
    22441 caacttcgag cctcagcttt tggtgaagag gtttatcctt cgatttatga aaaagcagct
    22501 atcctactta tacaattgat aaagaaacat ccttttcaca atgcaaataa acgtactgct
    22561 ttcttagcta cttttgtctt tttgaaaatc aatggacagc ttttagctat tgatcaaaaa
    22621 gatgctgtaa atttagcggt atatattgct acgtatgata aggatttcga tcaactaaaa
    22681 tatgaagtga tcaacactat aaagacacat acgcagccca tttaaggagt tactgtatgt
    22741 gtctttatag ggttattagc tgctatagcg aaataattga agtaaattac aaatagctat
    22801 aattcaaatc caactatata gaaaacgtaa attttctata tagttggatt aagataacga
    22861 ctatttattt tatcatattt ttactatacc ctatactttt acttggtata tttatgaata
    22921 atcgcaaatt tacccctata ctactagttt gtaagttaga ttaataaaaa tactaaagtt
    22981 atttgttctt ttattaaata cacctacaac ttataaattg atggtattta gataacacga
    23041 aagaataaaa ataacagctg atagtaaagt ttgttgacga ttgctactca atcgaaagtg
    23101 agttagtgcg tatgaacttg cttctgaaaa cttatgaaat gaaagacctt ttacatcttg
    23161 cagaaatcgg tactgggatt aaactaagca ataaaaacta atgttcccct cattctttga
    23221 acttctacta tttgatttat ttattctgat aaatgacgat ttcaccttca ggatcttcaa
    23281 atcccgtata gaatttattt aagatctctt cagtatcagt aataggatag gcgataggcc
    23341 acataccctt tgatggttag aataaactgc tcaaaatctg tatcgtatgt tggctttagc
    23401 atattaacaa aggctatctc caaattataa accataagcg ttgaatcgtt cttaacaatt
    23461 tcaattcttc ctaacccaaa gtcagaagat ataattaccg gacttgcctt acccttcatg
    23521 tgggttgttg agattccata tttaaaggga attgtcggta ctatcggcgg tctgaaaaac
    23581 atcccaaaaa taaaaaaatc tttcccctgc tagtctccta agtccctttg tagcggatat
    23641 ggcaccataa tctcatttta tcgaagtgag tcatggttta tctgttggcc aaccaaaatt
    23701 gatacaatct aacgatgcac cctctacggc attctagggg agtgcacttg cattcaccca
    23761 ataaaaaccg gcagttagca aagaacttat gtagtcgtat gttcttactg ttaacataca
    23821 agaggtctcc tttccacaat tgcttagcag taaacgggaa tttacaaagc taatctcttc
    23881 cattttctta tctttgttct aaaagtccca aatggagcaa ctgtatttac atgaataaat
    23941 ttatatactt cccatacagc cgttttggtt gcctcgtctg cccattttct tttgtgtgca
    24001 gtgaatagat cttcatcact cattgaatca atcatcatgt taatatcatc aatattacga
    24061 ttgagaattt cctctaattc tttcaatggt agatgtgcat aggtatttgt aaaccactta
    24121 tataactctc ctaactgatt ccatttaaat tgatcagaag gcgttttaac agataatcca
    24181 tttctttcat cattttccca ttttagaatt aaattcgtcc aaccaacttg atatgctaaa
    24241 ttttcagcag gtgttctatc cacttcctca actcttttat ccttcaattc ttcaggaata
    24301 gtttcaaatt ctaaaatata tttagtataa gtcctattta tttctttttt taattcttct
    24361 ttatttttat atgttctcat tatattacct ccataaattc ctaattgtct cctttaattc
    24421 atctataatt tcatccacga tattttaatc cgttctacaa tgcatatctg atatgcattg
    24481 actgtctaac tcatatgtga atgatttata aaatatccca acgtctcatc taaagaaatg
    24541 ttgatcgtca gcaaggagct aaaaaatacg aatatatttc ttttgtagta aaatcggact
    24601 ggtttgcata tcattttctt cgtaaatatc aaaatcatgt taactactat caattactaa
    24661 taaaaactac cactttaagc gttaagaact gttaagttta gttaagtgag gatagttcac
    24721 aaaatccatt gttaaacttc acgaaagtgg taaaactcaa aatgctttat ataggattac
    24781 agcatcgctc tttcttcgat tgcgcgttgg gtaaaaaata ttatgaagta aaaattgacg
    24841 atgacacgtt ccttacggct catcagatga aacagctaca aaaacgaaat gatcaactcg
    24901 aagaaaattt aattctaaaa aagcgattgc catattcacg cctcgctcga cgaactatta
    24961 aaggtcaaga tttacctgaa gatcatcctt tttaggaaat tgaagctttt tatgttgcac
    25021 aatgcattta taatactaca atgatgtttt caccagatat tcttctaggg tgcggtgtaa
    25081 tgaaacagat acacttgcaa aagaaaattc accgcgaatt tactaaaatt atgaataatt
    25141 atgttaatct tccgaaattt ttttccaaaa ttaagggatc aagctggtgt tatagattgt
    25201 ttaaggttgg caaaaaaata acttcttttt aaacaattat agaaaaccga tactctgtta
    25261 agttggttga cacggtgtcg atttttatga ctttcttatt ctgaaacttc actaacggac
    25321 agtacctaaa aagtctatag tatggacgtt tacgagtact attttatcaa aaatgctgct
    25381 catttcaact tatatgtttt agtgaactac ccccacttag cttggatagg ctacaattaa
    25441 ctagacagaa aaattaaggt gtgtagacta gaagaaaaca taccaggagg aatttttatg
    25501 tctaagagaa cacgaagaac tttttcacaa gaattcaagc aacaaatcgt caatctttac
    25561 ttagctggaa agccacgtgt agaaatcatt cgagaatatg aactaac
//
LOCUS       DACDKJ010000034        25502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDKJ010000034 DACDKJ010000000
VERSION     DACDKJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.034"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IV328_001607"
     CDS             133..3660
                     /locus_tag="IV328_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8937747.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IV328_001608"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IV328_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8937748.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IV328_001609"
     CDS             7448..7789
                     /locus_tag="IV328_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8937749.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IV328_001610"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IV328_001610"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8937750.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IV328_001611"
     CDS             9146..11566
                     /locus_tag="IV328_001611"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8937751.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IV328_001612"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IV328_001612"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8937752.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IV328_001613"
     CDS             13000..13737
                     /locus_tag="IV328_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8937753.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IV328_001614"
     CDS             13751..14566
                     /locus_tag="IV328_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8937754.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IV328_001615"
     CDS             14579..15226
                     /locus_tag="IV328_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8937755.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IV328_001616"
     CDS             15242..15898
                     /locus_tag="IV328_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8937756.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IV328_001617"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IV328_001617"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8937757.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IV328_001618"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IV328_001618"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8937758.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IV328_001619"
     CDS             18240..18758
                     /locus_tag="IV328_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8937759.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IV328_001620"
     CDS             18865..19365
                     /locus_tag="IV328_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8937760.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IV328_001621"
     CDS             complement(19645..20565)
                     /locus_tag="IV328_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8937761.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IV328_001622"
     CDS             20730..21743
                     /locus_tag="IV328_001622"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8937762.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IV328_001623"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IV328_001623"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8937763.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IV328_001624"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IV328_001624"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8937764.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IV328_001625"
     CDS             complement(23383..24144)
                     /locus_tag="IV328_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8937765.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="IV328_001626"
     CDS             24333..25427
                     /locus_tag="IV328_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8937766.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5384 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DACDKJ010000035        24804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDKJ010000035 DACDKJ010000000
VERSION     DACDKJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.035"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IV328_001627"
     CDS             949..1422
                     /locus_tag="IV328_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8937767.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IV328_001628"
     CDS             1431..1658
                     /locus_tag="IV328_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8937768.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IV328_001629"
     CDS             2294..2665
                     /locus_tag="IV328_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937769.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IV328_001630"
     CDS             complement(2921..3163)
                     /locus_tag="IV328_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8937770.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IV328_001631"
     CDS             complement(3195..4097)
                     /locus_tag="IV328_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8937771.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IV328_001632"
     CDS             complement(4110..4298)
                     /locus_tag="IV328_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8937772.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IV328_001633"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IV328_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8937773.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IV328_001634"
     CDS             complement(4913..5806)
                     /locus_tag="IV328_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8937774.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6821)
                     /locus_tag="IV328_001635"
                     /pseudo
     CDS             complement(5884..6821)
                     /locus_tag="IV328_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            complement(6855..7205)
                     /locus_tag="IV328_001636"
     CDS             complement(6855..7205)
                     /locus_tag="IV328_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8937775.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7238..8140)
                     /locus_tag="IV328_001637"
     CDS             complement(7238..8140)
                     /locus_tag="IV328_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8937776.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8133..8990)
                     /locus_tag="IV328_001638"
     CDS             complement(8133..8990)
                     /locus_tag="IV328_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8937777.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9324..10133
                     /locus_tag="IV328_001639"
     CDS             9324..10133
                     /locus_tag="IV328_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8937778.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10173..10670
                     /locus_tag="IV328_001640"
     CDS             10173..10670
                     /locus_tag="IV328_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8937779.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11055..11128)
                     /locus_tag="IV328_001641"
     tRNA            complement(11055..11128)
                     /locus_tag="IV328_001641"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11092..11094),aa:Ile,seq:cat)
     gene            11315..11638
                     /locus_tag="IV328_001642"
     CDS             11315..11638
                     /locus_tag="IV328_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937780.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11802..12056
                     /locus_tag="IV328_001643"
     CDS             11802..12056
                     /locus_tag="IV328_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937781.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12126..12371
                     /locus_tag="IV328_001644"
     CDS             12126..12371
                     /locus_tag="IV328_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8937782.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12447..12812
                     /locus_tag="IV328_001645"
     CDS             12447..12812
                     /locus_tag="IV328_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937783.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12873..13436)
                     /locus_tag="IV328_001646"
     CDS             complement(12873..13436)
                     /locus_tag="IV328_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937784.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(14004..14204)
                     /locus_tag="IV328_001647"
     CDS             complement(14004..14204)
                     /locus_tag="IV328_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8937785.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(14956..15669)
                     /locus_tag="IV328_001648"
     CDS             complement(14956..15669)
                     /locus_tag="IV328_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8937786.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15662..16747)
                     /gene="uxuA"
                     /locus_tag="IV328_001649"
     CDS             complement(15662..16747)
                     /gene="uxuA"
                     /locus_tag="IV328_001649"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8937787.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(16764..17207)
                     /locus_tag="IV328_001650"
     CDS             complement(16764..17207)
                     /locus_tag="IV328_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8937788.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(17241..17594)
                     /locus_tag="IV328_001651"
     CDS             complement(17241..17594)
                     /locus_tag="IV328_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937789.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(17706..18107)
                     /locus_tag="IV328_001652"
     CDS             complement(17706..18107)
                     /locus_tag="IV328_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937790.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(18144..18653)
                     /locus_tag="IV328_001653"
     CDS             complement(18144..18653)
                     /locus_tag="IV328_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8937791.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(18675..19532)
                     /locus_tag="IV328_001654"
     CDS             complement(18675..19532)
                     /locus_tag="IV328_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8937792.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(19550..20383)
                     /locus_tag="IV328_001655"
     CDS             complement(19550..20383)
                     /locus_tag="IV328_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8937793.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(20397..21194)
                     /locus_tag="IV328_001656"
     CDS             complement(20397..21194)
                     /locus_tag="IV328_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8937794.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(21227..21511)
                     /locus_tag="IV328_001657"
     CDS             complement(21227..21511)
                     /locus_tag="IV328_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8937795.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(21508..22509)
                     /locus_tag="IV328_001658"
     CDS             complement(21508..22509)
                     /locus_tag="IV328_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8937796.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(22511..23413)
                     /gene="gnd"
                     /locus_tag="IV328_001659"
     CDS             complement(22511..23413)
                     /gene="gnd"
                     /locus_tag="IV328_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8937797.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            23577..24425
                     /locus_tag="IV328_001660"
     CDS             23577..24425
                     /locus_tag="IV328_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8937798.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         7256 a         4909 c         4015 g         8624 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt tttttcgata cgattatctg actgatctag attaatcact
     6601 accaaagaaa atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct
     6661 tccggatatt cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc
     6721 tcaatctctt cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt
     6781 tctattacat tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga
     6841 ctttctcgaa actattagct gaagaagcag ttctttaatt tgatattata atcgagtact
     6901 gcgatttcat tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt
     6961 ttaccgaaat aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt
     7021 ttagtcgcat attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt
     7081 gcaaaagcac ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt
     7141 tttgtatttt ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt
     7201 tccattccta ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt
     7261 ttggttgaac ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg
     7321 tcatccctgc atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg
     7381 gtttcacatg tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac
     7441 gttcacctgc tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc
     7501 aacgcggatg aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat
     7561 ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt
     7621 catctaacca ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt
     7681 tagctgaaat tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta
     7741 cgattgaatc accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt
     7801 cgcaatccat atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa
     7861 tacgataata agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt
     7921 tattgatttt caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta
     7981 aaagctgttt actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag
     8041 acgggttgca attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg
     8101 tgttacaact tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt
     8161 agagtactgt gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta
     8221 ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta
     8281 tactgacgtt catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc
     8341 atgttccatt ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct
     8401 tggtcgtgat agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta
     8461 atttcttttt tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag
     8521 tcatcggaat cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg
     8581 ccgattgttt ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac
     8641 acatctgaat ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa
     8701 gaaattctta agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata
     8761 tctttttcca ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct
     8821 tccaatcctt ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt
     8881 cttgttttat tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa
     8941 ttttcgtctg aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact
     9001 cctttttcta atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa
     9061 agcaaaatat atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat
     9121 cggtaagcgc taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact
     9181 actatatata gccacctttt tcttaagaat gttttttgaa aaaattcata atgacaaagc
     9241 aagttagttt cattttttta aattctcatt taatcttaag gtaacaggaa aatagatatg
     9301 taaaaagaaa gtaggaagat aaaatgaaac tagcagcgat tgatttagac ggtaccttgc
     9361 ttgatcgaaa tggaaagata ccagaagaaa atatccaagc acttcggaaa tttgatgaaa
     9421 atggcggaat cgtaaccgtc gctaccggaa ggaacagcat ttctgctaaa gatatttttt
     9481 ccgaattagg aatcggtggc tatttgattt cttccaatgg ttcactgatt gctgaaatga
     9541 aggatggaaa aatggcgaaa gttctcagac gttcaaaaat agatagcgat acactgagaa
     9601 aagccttctt ctatgctcaa gaagcgaaaa tatccatcat tgctagccga gaaacccaag
     9661 atgatcagct gacattcgat gaatcaaatc tagtcaaaga tgacccttat tatgaacatt
     9721 tcaatttaca gaaccattct tttgatcaaa ttttaaatat gttggaagat ccttctctta
     9781 gctatcttaa attagcatta acagataaga gtgaagaaaa actacagaag ctacaaagag
     9841 atctgcaagc agatggtatc caatcagtat tctctgatcc gcatttctta gaaatcacgc
     9901 ctacaggtat cactaaagct cattcacttg tttttctggc ggattacctt ggaatcgaat
     9961 cgagtgacat catggctttt ggcgatcaag aaaacgacat tgctatgttg aattacagcg
    10021 ggttgagtat tgcgatgggg aatgcacagc gacaagtaaa agaaattgca aaccaagtaa
    10081 cactgacgaa tgaagaagct ggtgttgctt atattttaaa aaacttctat taaaaagaat
    10141 aaaatgacga tatgaatgta ggaggaaaga aaatgttagt tattattgat atgcaaaatc
    10201 atattcttga tccaaacagc gaattttata ttccagagtc agatcaattg gcagaacgta
    10261 ttgctcaaag attagctaaa gcacgccaaa ataatgaata tattctcttc acacgggata
    10321 taccagttga actgaaggac aaagaagaag aacgagaaga tttgcaattg atccctcttc
    10381 tttctccaca agaaaatgaa cgagtgatta aaaagtatta ctttacgatt ccacctgaaa
    10441 ctttagctga aatccaggaa actttatttg aaagcaaaga agaacaaaaa gagatcgaag
    10501 tagtcgggat tgaaacaaac ttgtgtgtac tctctaatct gataggcctg cagagtgctt
    10561 ttcctgaagc agactttttt gttgatccaa tgttagtctc cagtagaaaa cacggagaat
    10621 ccgctttaga gctgttgaaa gatttcaacg tctctatcgt cgaatcataa aaatatctat
    10681 cttcatttac gatatttaac acacaataaa aaaagcacgc agaactgttt tagtcctacg
    10741 tgctttttct atcaaagtag attggctttt ttatgacaca gagtgaaacg gcaggctcac
    10801 gacctctttc ttaaatggtg ttcaatcagc tgaccctcgg tattagctta acaaacggca
    10861 aaatatgagg aactattttt ccgtttgtta agctaatact caggtcaaaa cgctgatttc
    10921 acaacctctt ccttaaacgg tattcaaaag cttgcatctg cagtaataag aacagccaag
    10981 taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
    11041 ttcacaacct cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt
    11101 ctgctctaac caactgagct aaaggtacat cataaataaa atttattttt gttacttttc
    11161 aactatagca cgaatgacaa actctcacaa gtttcaaaat ataagtaaaa taatattgtt
    11221 attcttattt cttttacttg tgtcataact attttgttac tttaattttt atttgcttat
    11281 tttagaaaaa atgttattct tttatttatt ctcaatgtta ggagtgatta ttttgccatt
    11341 tattgcgatt ttattcgatt tagttgcggc agcagcttat tttctccaat taaattatca
    11401 aacctcagga attctgttta ttggaatgat ttttcaaggt gtgattaccc ttttgcttct
    11461 tgtcatgatg ctcagctata aaggaaaaag atatgcccgt attcaaacaa aagtatttgt
    11521 taaatatgtt tccattcgct atgggatcat cattctttcc tttttagtga atgcgatcgt
    11581 cctcttttta tatgtattga attacttaga tatcaatcca ttgatttttt ctcgatagat
    11641 aagacacatt cagcggaaca gttcctgctt atcagcagaa aactgttctg ctttttcatt
    11701 tttcattgat ctattcttct tttattttcc tagcatttca tttgtgatgt atgcaataac
    11761 atgttatctt ttattatatg aatcgttgag gagtccgtta catgattacg ttggtgagag
    11821 ttttgttttg ggtgcctgcg gtcgcactgg tcgcaagtat tgtttatctt atgaattgga
    11881 acaaagaacg gttttattta gctattttga ctcttccggc tatctacttt atgtggaaag
    11941 tttttaatta caactacttt gaacccgata gtgtttttat agaagaatta tcaggactgg
    12001 ttttgtcttt attgatcgtc attctttatt tgatacgttt aaataaaaaa cattgagtac
    12061 tattgattgc atgtgtagct ttctatagtc attataacta ttctactata atgaaaggtg
    12121 attaaatgag tatcgaagaa gctattttac tggatacttt acgttgtcgt ctgaatttct
    12181 taagaactgg ccgatcttta tctcaagtag ctgtttctag cgcgttaagt attcctttat
    12241 tcaaatatcg taagtacgaa gaaggagaag aaattccaag catcattgaa ttaatctata
    12301 tcgctgactt ttacgatatt tccgttgatt acctaattgg tcgctcagaa acagcaagca
    12361 aactgaagtg attttttcca gccttggtgc tggttttttc atttaacgtc taaggttaac
    12421 aatctatata taaaggagac atttctatgc gattatggca tgaaaaattg atttcaaagc
    12481 ttcctcgtca acagctttta ggacaacatc gggaatgctg tgcattaaga ggaaatggct
    12541 ggggaaagaa acatgctact gtggattatg tttttcacta ttctccttat agattattcc
    12601 aatatcatca gcttgtcatg gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt
    12661 ggaaaaatcc tatgtatcgt ggcaaatcat gcgaagcatt ttttgattta cctactgtag
    12721 agcgcaacgt gccgatttat ccggaacacg atgaagttta tctagcagaa tgtttagcaa
    12781 atttggagca aaaaggaatt tatcttacct aatataacta gaaaagcctt ctatctcctc
    12841 ttacttcatt aaaaagcttt aaccatctta gatcaagcta aaatgtcgta agttttaggg
    12901 ttatcttcat cctcacgtgc aaaactaaaa gcgtactcta gttttggttc atcgccaact
    12961 tccctagaca taaatgaaca tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct
    13021 gtatacgatg cacagcacag attgcttctt ttgatctttc ttttgatttt tctgttgctt
    13081 cttctacacc ttcttcgatt actacatcga tgcttacaat tgcttctgcc taatattccc
    13141 atgcatcgtc gatttaagcc tctgtagtca ctgtgatcgt ttccacattt taactcctct
    13201 gtcgtggctt cagttgattt tgtgatcagt tcagttgcta agccagagtc agaacaacct
    13261 gccattgcta gcgttaatat tgatagtaaa aatagttttt ttatcataag gttccccccc
    13321 tgaaaataga ctaaaataat aacgctttct ttttttgttt tcagacgaaa attaataatt
    13381 tctaaatatt ctttaaaaaa gcttataact attatgcctt ttttaatata taacacagtt
    13441 atttttaaaa ataagaatgc taaatcactc tacaataacg gcgaaagcac aaaaaataaa
    13501 aaaattatta cgttattaaa aaaacagatg aaagatcagg ttttattaga tctcattctt
    13561 tcatcacttg aagggattta atgaactact ttttcctctt tgtcgttttt atatattcgt
    13621 attccttcaa acagtaggat agcctctttc aatgcttgat atttgaactg tccgttactt
    13681 aaaaatttag atgtgccgcc ctcttcctag aacatattct tttttacttt ttcattaatt
    13741 gacagaccaa tcgaacgaaa ctgtaaattt cgataggata atgattggtc aaactcattc
    13801 attttcaaga catctcgatt catattcaag ataggaatct ttttatcaac atcactttat
    13861 tcgtattttc ctctaccata aaagcttcac gctccacaaa attgttttat acactactat
    13921 tcagcccatt caacgaccaa tgaatttttt cgtacaaaaa aaccgtcttt tctttcgaaa
    13981 agacggttaa aagtcaattt ttattaagct ttaacaatgt ttgttgcttg agggccacgt
    14041 tgaccatctt caacatcgta agacactgct tggccttctt ctaaagtttt gaagccgtca
    14101 ccttggatag ctgaaaaatg tgcaaataca tcatttccat cttcaccagt gataaagcca
    14161 aaacctttgt ctgcgttgaa ccattttact gtaccgttat tcataaataa attcctcctg
    14221 atacgtatca atgtacgttt tttcgttgca ataaatattt gataggcaaa aggagtttta
    14281 tctatgtaga tatatcgctc aaattacgtt tcaaaataga ttactcattt aatatatcat
    14341 gctttttcta ataacacaag gataatgcaa tgaaatataa aattcattgt cttctattgt
    14401 cttcgaatcg aagaaaaagt tgaataaccg cgattgattc actttttgtt ttcttgttta
    14461 tttgctttta tctgcaactt ttgactttaa aatttcttaa ctatgaaaac ctttcaattg
    14521 gtggtatact tataaatgga atttcgttat caacgcgttc gttgcctttc agcaagtatt
    14581 taaagaaagg agaatggcta tgacattcta taacaaagat atacaatatc gagtaacctt
    14641 ggatacagat ttgaatctat ttgtcgtttt tgacaaagaa aataccaatc atgtagcgac
    14701 aggtgttacg atcgaacaag cagtgcaaga attaaaaaaa tctgcataag gagtggaaac
    14761 cctcatatgg acgattctcg ttcttctctt taaacgcatc tactggtaga taacagtaga
    14821 tgcgttttgt gtttgttcta aattgttcac tacaaataag gtagatactt caaaataaaa
    14881 aattacttct ttttcatctg ttccctcagt cgtgcaggac aataaataaa gaggactaga
    14941 aaatagtcct ctttattaga gatacaaccc tttactttct aaaaatcgtt ttgcttgatg
    15001 caggtcataa aattcatagt ctgctttatt ggattcatat aaacttacat ctactttcaa
    15061 cttatgcttc ccattatgat cgggcaaccc tatcaaatga tctttgatga gaaaagacaa
    15121 aatgcccgct cgccttgctg cttcgatccc tgcaatcgag tcttcaaata caagtgcttc
    15181 acttggagga atattcattt ttgaacaagc tttcaaaaag atctctggtt ccggctttcc
    15241 tttttttaca tcatcccctg aaatgatttg atcaaacaag gcatatagat tttccatttt
    15301 taaatacatt tctactttta cccgtgtatt agacgaagcc acgattgtat ggattcctct
    15361 ttcttttgca aaagataaaa tttcttctgc tccttttttc gtatatactc tcttatcttc
    15421 tttcacgatt ttttctttat attgattgat tgcttctcgc catttgatat atggaacatc
    15481 ttctccgtac aaaatgccca tttgttctct tatcgccatt tctgtttggc cagttagtaa
    15541 ataatagact tccttacgca tttcataatc atacttttgt gctgatttta cataagcacg
    15601 ataagctaaa cgtccagttt ctaataaaag gccatccata tcaaagatga agagcttgat
    15661 tttattcata atgctctccc agtggttttt ccttacttat cgcctcatat agaccttgca
    15721 tgtaagtgag tcccattgca cgatcataca ggccatatcc aggcatagcc acttcacccc
    15781 acacggttct accatgatca gggcgtatag gaccgtcgaa cccttcttgc accaacgctt
    15841 tcataatatg atacatatca agggagcctt ctgtagaggg atgtgccgtt tctttgaact
    15901 ttctttctcc taaaaactcc acgtttctga agtggacaaa atggatgcgc gaacctacct
    15961 catggatgat ttcaactaaa tcgttttccg gatttgcccc taaagatcct gtgcacagcg
    16021 ttagtccatt atatggagaa tctactgcat ttagaatttt cttcaagtcg ccaagatttt
    16081 tcgtcactct aggaaaacca aaaagttccc atggcggatc atctggatgg atagccattt
    16141 ttacatctgc tttttcgcac actggaatca ctttttctaa aaaataagtc agattttcaa
    16201 ataattcttc ttcggtcatt ccttcataca gtttttccag ctgagcgaat tttttcaacc
    16261 gttcttcttc ccaacccggc aatttgaatc cctttgactg gctgtgtatc aattgataca
    16321 tttctcctgg ctccatctga tcgacgattt tctgatcgta taaaagagaa tagcttccat
    16381 cgttgttttc attaaacaaa tcagttttcg cccatccaaa aattggtttg aagctataac
    16441 aaatcaatcg aaccccacat cgagacaagt ttttgattgt tcgtatatag ttatcaatat
    16501 agtaatctcg ttcatctgtc cctgccttga ttgcatcgtg gatcgctaca ctttcaattc
    16561 caagcagttc cagtccttgg ctctcaacag atgctttcaa ctcctcaatt tctgaaatct
    16621 cccaaacatc tccaggcatt ttagtcagca aagttccaat tatcccattt attcccggta
    16681 tttggcgaac atattttaat gggatcgaat cccctttttc accataccat ctaaatcccc
    16741 acttcatctt ttcagccttc cttctaaaaa tcgtcctcat ctaatttttt ttcttccgct
    16801 gaagcatgcc aaacagttaa attatctttt ccttgtttga caatttgttc caccgcttct
    16861 tctatcggtg tgtgaagcat ctgatgattg attgcttcta acaacattgg cagattcacg
    16921 ccccctatga cataagtggc tgctttcttt tcttcagtca gttcattgac tgccaagagc
    16981 gcttgattgt acggacttgc gctaattaga tcaacaaata ctagcatggg ttctgctgtg
    17041 ttgatcatag ttaatttttc tttgaccttc tctccgaatg tttggatgtc ctccccagac
    17101 tctaagcaca ttgtttcaat atggctcgat gtcccgacaa ttacttccgc cgcatctttc
    17161 agtccttcgc tcaacttacc atgtgtaacg atcatgattt ccaacattag tcttctcact
    17221 ccttgttttg cactttttac ttatcgaaaa agagcaataa tctgattggc attttgccaa
    17281 ttaaatgttt tccctcgtaa atctgttttg gtcaaataga agaactgcgc ttgctttttt
    17341 cttgcactaa aaatataatt gacgtttttt tctttcaact tatcaacttg tttcagccac
    17401 tgttctttat ttgaaaaata atttcccgaa aactgtagcg gtgtatctgc cccaacagag
    17461 tctagataaa aaagaatagc ctcttttttc atacccgatc tcacgctgtc taagcccttt
    17521 tctccataac agccttcatc aacaaacgca tacgctacac tcggatcatc aatcgtacgg
    17581 atcatttcta gcatcgttaa tatggatgaa gtatttcgaa taaaagtttg tctgctaggc
    17641 catccgcgag ctgcttttcc caataagaaa aagtagctcc caaatgctag tacagtgata
    17701 cctgtttata cggatagcag atcgaatacg tgatgaggat ttttgttata tatcatcatt
    17761 ccccaccaac ctaaactgaa caacaaaatg aaggacaata atatagagta gattgttttt
    17821 ttcctttggt ctttccaatc aaacatgaaa taacttccta atgcatgaac gggtgtatcg
    17881 taataagtac agataacttt ctctgctttt tcgatatctc caacataaat attttttgat
    17941 cggtaggcta gcgtatcgta agcaatgact gtcacatctg ttctcatact gtaaatatct
    18001 gatactaatg ctgaaagaaa acgctgttta ctcttttttg tacggcgctt acggtaaata
    18061 tagcgataac gaaattccca atcacgaaac agctcgttct tcttcatttt ttcgccctct
    18121 ttatcttttt tctaagcatt tgtttatgac aaatcaaact tgttcaatac atcttttaat
    18181 cctgttttag gtgcagatgg agtggcttga aaataaatat tctttacgcc atattgttca
    18241 ttcatttctt tcaatttttc agcatctgat ttatttaaat aaacagattt cgtcactaaa
    18301 agatctgtat caggattttt ttgagcaatc cctccaatat tcaattcttc catggggatt
    18361 ccgtgtttta ctaagtcgta aatcgtttgg actgtttttg cgatcaaaat acaattgtat
    18421 tctttaaatt gatattcatc ccaatagaat ttagctttct ctggtgtaat gacaattgtt
    18481 ttttgtcctg tacggcttcc tgcatttttg taaagatctc tcataaattt atcttttgct
    18541 acaacttcgt ctacaacaaa aatggaattc actccacttt tttgagataa cgttaccatt
    18601 acttgcccat ggatcaaacg ctcatccaca cgcgctaaat tgactacacc catcttattc
    18661 acgttccctt ctttttataa aataccgatt cctgctagca caattaatat tccagtcagc
    18721 cataataatg cttgtgtgac tttcaatcct tttttcgtat agaacaagta cacacccatt
    18781 acgacagcta aaggtaatat ccctggcacg atttgatcaa gaatttcctg gataacaaac
    18841 tcacgtccgg atatcgcaaa tttcaatgaa gattctactt ttacatagtt tcccgcaagc
    18901 acccccatca taaacaaccc taaaactgat agcatttcaa tcgctgtctg aacaccagtg
    18961 ttttgcttta ggcctgtagc attacggccc atagtgaaag cttgtcttgc gaaaaatacc
    19021 cctagtaaaa cctgatacaa cccaaagcag ataaaaggaa aaagtgcgcc taaaggactt
    19081 ccctgttgtg cccaaggtac cgcgattgcg ataaaaatat attggacggt tcctgaatcg
    19141 attgcatctc cgattcctgc tagtgccccc atcaatcccg ctttcgtgtt atacatcaag
    19201 tcatcctgca tcaagatctc tttttcttca tactcttctt ctgctctttg ctgttccata
    19261 gaagacatta ggccagtaat cacaccgcca ccccagctta attgagtgtt gaagaacagt
    19321 aactgccgtt tatatgctgc ttttaaagag tcttcatctt tataaagttt tttcaagatt
    19381 ggcatcattc cgtatagtag tgatggcgcc aagtaacgtt caaaagagtg aggaatttcg
    19441 tttgcaaaat gccatctcag ataggctttt gttacatctt ttttcgtgat ttcttcagga
    19501 gcacctgttt gattatccat tatttcagcc atttcccatt cctcctaaat taaaatccgt
    19561 catcatagtc atcttcgtca tcaaaagatg ctgaattgtt attgcttgtg gcaggctgta
    19621 cttgtttccc tttttgcgac tggacaaaga tcaaagcgat aataatccca aaaatggcat
    19681 aagtaaccat cgtaatatct aaagatttca aaacaacact cataaaataa gcaaggaaga
    19741 aaaagaccag ataatctttt cgaccgataa cgtatacagt tgtcgcgata ccgattgccg
    19801 ctaaccctcc acctactact tcaagaatgt ggataacgac tgttccctct aaagctttga
    19861 ttacatcagc gatcaatggc gcccctaaat ataaagcaat gaaaacaagc ggaacgaata
    19921 ataaaaatga ggcaaccgtt ggataaagaa taattgatcg ggccatagct tttgtattca
    19981 aatcttctac agctttgtct gtatatttac ctaaaaaagt attgatgaaa aaacgaaact
    20041 gatacaagta acttccgagc aatccgactg gcaccgcaac ggcaatcgct gcttctggcg
    20101 acagatcccc taataatgcg actggtactg cgatagctgc tgcaatagat ggttcagctg
    20161 gcattgatcc tcctggagaa aaaaccccca tataaatcat ttgcaaagcg gctgtaacaa
    20221 tcatcgcttg tgcaacttcc cccatgatca acccgacgac taatccagtc atcaaaggag
    20281 aaaaacgtaa ggtcaacgtg gctcctccac ctaaccagcg ggacataacc gcggctaccc
    20341 ataaagcaat caacaaactt tgtactacac ttaatgtttc catgtgtcct cctccttcag
    20401 atctttctga tataaccttc cagttccatc attgactgta tcagtgattt ctgtgttacc
    20461 tctataggaa tcaaatgcac tttctctttc gaatcaataa attcagccat tttatctatt
    20521 atctgtttat ctttcggaaa taatcccatt tgttctagag aaaccggcag ttctaattcc
    20581 cgatacaaag gatataactc gtctattttt tcccagcgac cttctagtgc cagttgatag
    20641 aaaatcccat acgctacttt ttctccatgt agaaaacgat ggctttccgg aatatattta
    20701 ctgatagcgt catgcatagc atgtgctgcc acatttcttg cgtatttatc tcctaatcct
    20761 ccaactaaac cagcaacagc aaagataatc tctgaaagct gtttgaactc cgatgtcagt
    20821 tttctttgtg ccatatcttg gattgcttta gcagaatgat tcaaaatctc ttctttgcag
    20881 atcttcgcag tatcttgtgc tagcttcaaa aatgattcat cttgcaaaaa atcttgttct
    20941 aagatgagct cagattcata ccattttgct agtgtatctg ctaacccggc aatgaaatat
    21001 ggaacagggg aatcaatcac taattgggga tctgttataa aaaaagcagc ttgtctgtaa
    21061 aagtgttctg ttttcccttc tgcttgtcca ttctccttat acatgacagc aagaggtgtc
    21121 catggagcac aattactagc taaagtaggg atgactccga aatcgattct tgcaagattg
    21181 gcactataac ccactagatc ggtcagtttt cctccgccaa cgccaactac gaaatcgatt
    21241 tcattttttt gatgattgta gctattcgtc tggcttcagc ataactacat tctccagaat
    21301 aacgatgata aaaaaattga atcgtttcgt cattcaaaaa tactaattta ggtagcgcct
    21361 tttcccatga taccgttccg tgggtgacca gtactctttt cgcgccgtat gatttcaaga
    21421 gatcagaaac ttttgaaata ccaccttctt catataaata aaattgaggg cctgttttca
    21481 cttttaaacc gttcaccatt ttcttcatca tccttttata tagttgctct ttgtctggac
    21541 agaacgttca ggaagaaggt gctgactgat ctgttcgaca tctctgacac atttttcacc
    21601 cattgcttct aaacattccc ttgtgtaggc cgatatatgt ggcgtaatca ctacttgttc
    21661 aaacgctaaa tacggatgat cttgacgtcc cggttcttct tctaaaacat ctgtggcata
    21721 tcctgcgatc tttccgcttt tcagaccatt aatcatcgct tcttcatcaa ctagagcgcc
    21781 tcttgcacta ttagatagat aaacattttc tttcatctga tcgacttctt tatcagaaat
    21841 catgtgatag ttctcttctg tcaaattcgc acataaacaa atgatatctg attgttccag
    21901 taattcttga agttctactt tttttgcacc aaagctttcg atgtataaat ctgatttgta
    21961 aggatcataa gctagaacat cacagcgaaa accgttacgc aaaatttcga ttactccgct
    22021 tccggtattt cctgcaccaa tcacaccaac tgttttgtta aacagcgtcc taccgacgaa
    22081 ctccgcccga tcttcccatc gatcgtcctt gactcttgct tgtgcttgtg tcgtgcgtcg
    22141 aagcatggac aacaaattag ttatattatt ttctgctaca gcatttcgtt caactaaagc
    22201 aggtatgata gatacgattg tctggtgttc ttttgctgct tttaaatcaa tattgttgta
    22261 accaatacca tgtcgggaaa tcaatagcag ttcatctttg ttttcaaaaa aatctcgtcg
    22321 aaaaaatggc gtaacacttg caataatgat attgaatcct ttaagtaact ccgctagttc
    22381 ttttcctgga atctcttgat caacagtaaa gaaatgaacg tctccaactg actttaatct
    22441 ttccaagtgg tgtggaaaga ctcttccgaa actgctggaa ttaacgattg ctatatgata
    22501 atctggcatt ttactcaccc ttttcaacta tttcatgtcc gccaaaccca tttcttagtg
    22561 ctgaaactac ttttgctgaa aaactatctt cttcctgtga aagattcctc atgaacaaac
    22621 tagttgcaat tactggaact ggaacagcca tttctagcgc tgtttcaacc gtccattttg
    22681 cttcacctga tgcagaaacg attccccgat aattacttaa attgggatgt tcgtttaact
    22741 gatcttccat gatttccatc agccagccgc gaatcaccga accatgattc caaacacgtg
    22801 cgacttgttc caagtcaaaa gcatactcac ttttttgtac aagttggaat ccttcaccga
    22861 ttgcttgcat cattccatat tcgattccat tatggatcat tttcaagaag tgtccgcttc
    22921 cggatgcacc agcatacaga tatcctcttt caattgctac atctttgaat aattgttcca
    22981 cggatagaaa agtttcttta tctcctccaa tcattaagca ggcttgatgt cgtgctcctt
    23041 cgattcctcc agaagtccca caatccaagt aaccgatttc atttttctga aactcttctg
    23101 ctcgacggat tgaatcttta taataagagt ttccgccttc aataataatg tctcctggtc
    23161 cgagttttgg aaaaagtttt cttaacatgt tttcggttat ctctccagca ggaagcatta
    23221 accagatgat ttttttctgc tcaaaagtag ctagaaattc agagatctct tgataagcag
    23281 gaatgtcatt ttctcttgcc tgactaacag ctgtacttga aatgtcgagt ccttgtactt
    23341 tgtgatggtg ctcttttaag tttaaagcta aattcaatcc catttttccc aaaccaatta
    23401 caccaatttc catgatagcg cctccatttc ttataccctc attatacaaa aaaatttttt
    23461 tgtaaataga ttttacaaaa atattttttg tttgattttt tcctactctc cttgtatctt
    23521 taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga attctcatga
    23581 gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca aaagaacagg
    23641 ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct attagcaatt
    23701 tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa aaacttggtt
    23761 ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat ctatctgcta
    23821 tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg caaaaggtgt
    23881 tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa aaggatctaa
    23941 aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt gttggcggtt
    24001 cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc gcaacttctc
    24061 acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa aaagactgtg
    24121 ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct gaaaccgtaa
    24181 gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc cttgctaaac
    24241 ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct gaagctttag
    24301 cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg atgttttaca
    24361 acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct gaaatcaaag
    24421 aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca ctaccaaata
    24481 tttggtagtg gcttttttgg ttctgcaagg gtggtacaga acatcaatag tcggtgggtt
    24541 agaacgtatg tcatcgcatc tttggagaag gttgtattgg tattattcga gttacttgtt
    24601 agatgcgata acaaagttag tatacagtag taaaaaattt atcttttctt ttttttatta
    24661 tttttgtttc ttattttcta aaaaattaaa aaaaattttg gtgaattaag tatttccttg
    24721 ataacactgg cttttggcct cattttaggt tatcatttgt aattttattt cacagttgat
    24781 atgattgtta tgtaaaataa aaaa
//
LOCUS       DACDKJ010000036        24789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDKJ010000036 DACDKJ010000000
VERSION     DACDKJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.036"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..258)
                     /locus_tag="IV328_001661"
     CDS             complement(<1..258)
                     /locus_tag="IV328_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937799.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="IV328_001662"
     CDS             complement(274..924)
                     /locus_tag="IV328_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937800.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="IV328_001663"
     CDS             complement(935..2107)
                     /locus_tag="IV328_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8937801.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="IV328_001664"
     CDS             complement(2126..2998)
                     /locus_tag="IV328_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8937802.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="IV328_001665"
     CDS             complement(3020..3940)
                     /locus_tag="IV328_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937803.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="IV328_001666"
     CDS             complement(3940..4209)
                     /locus_tag="IV328_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937804.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="IV328_001667"
     CDS             complement(4206..4586)
                     /locus_tag="IV328_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP8937805.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="IV328_001668"
     CDS             complement(5053..6516)
                     /locus_tag="IV328_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8937806.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="IV328_001669"
     CDS             complement(6580..9597)
                     /locus_tag="IV328_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8937807.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="IV328_001670"
     CDS             complement(9748..10587)
                     /locus_tag="IV328_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8937808.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="IV328_001671"
     CDS             10732..10977
                     /locus_tag="IV328_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937809.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="IV328_001672"
     CDS             11040..11462
                     /locus_tag="IV328_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937810.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="IV328_001673"
     CDS             complement(11556..11873)
                     /locus_tag="IV328_001673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937811.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="IV328_001674"
     CDS             complement(11900..12106)
                     /locus_tag="IV328_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937812.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="IV328_001675"
     CDS             complement(12087..12269)
                     /locus_tag="IV328_001675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937813.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="IV328_001676"
     CDS             complement(12283..12633)
                     /locus_tag="IV328_001676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937814.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="IV328_001677"
     CDS             complement(12659..12961)
                     /locus_tag="IV328_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937815.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="IV328_001678"
     CDS             complement(12964..13197)
                     /locus_tag="IV328_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937816.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="IV328_001679"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="IV328_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="IV328_001680"
     CDS             complement(14628..15743)
                     /locus_tag="IV328_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937817.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="IV328_001681"
     CDS             complement(15749..16147)
                     /locus_tag="IV328_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937818.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17176..17562)
                     /locus_tag="IV328_001682"
     CDS             complement(17176..17562)
                     /locus_tag="IV328_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8937819.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17559..18080)
                     /locus_tag="IV328_001683"
     CDS             complement(17559..18080)
                     /locus_tag="IV328_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8937820.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="IV328_001684"
     CDS             complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="IV328_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8937821.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19149..20564)
                     /locus_tag="IV328_001685"
     CDS             complement(19149..20564)
                     /locus_tag="IV328_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP8937822.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20533..22686)
                     /locus_tag="IV328_001686"
     CDS             complement(20533..22686)
                     /locus_tag="IV328_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP8937823.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGE
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22944..23180
                     /locus_tag="IV328_001687"
     CDS             22944..23180
                     /locus_tag="IV328_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8937824.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23199..23414
                     /locus_tag="IV328_001688"
     CDS             23199..23414
                     /locus_tag="IV328_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8937825.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24117..>24789)
                     /locus_tag="IV328_001689"
     CDS             complement(24117..>24789)
                     /locus_tag="IV328_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8937826.1"
                     /translation="FHDKVFKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNG
                     IELAEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLL
                     LAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYG
                     RISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKL
                     KESTV"
BASE COUNT         7048 a         4923 c         3782 g         9036 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc tctaaacata
    16921 taggattaaa tctaagtact ataaaaggtt aaaaagtgaa gaatatttaa tagaaaaact
    16981 aaatttactt gctgctttcc ccttttccat taatacaaca atcagagagt gtcttttttt
    17041 cgaaaaaatt agaataaata aaagacacgt agttttttta tctacatgtc ttccatcgac
    17101 tacaagttat catttctcaa aacgtattta gcttttaatt gattaatcgt ttcacgttgt
    17161 ctacacattg cctaattaag attttcttat tagaagcatc aatcccgcaa taatacctaa
    17221 aactccagga attacccaca ataatgttaa aagaaaaaat ggaataatag ataaaatgag
    17281 taaactaata ccatagacca ctgttttttg attttttctt actaaaaaac ctattatgaa
    17341 aatcattatc gaaaaaacta gggtaattcc tccaaataaa acaacattcc cgcttagatt
    17401 gactaacata tctatatcct tactggttag ttctgtagta tttaaagtag ttgaatcaat
    17461 agaagataga aacataccac ccatcaatgc taaaagacta cataaaatcc ctgttattcc
    17521 actaattaaa atcaaaataa actctgtttt tctcttattc atttaattct ctccagatta
    17581 agtgttttag aattagaaat tttttgacca tagttaatag cttctaatga acgaataaca
    17641 accactgacg tgttagaaaa ataatcccca atatgttgtt tcttgggcgt gaaagcagcc
    17701 accaaatacc caatttgaaa tacaaaattt tttaatataa acctacatat acactcacga
    17761 ataaagatag tttgccaact tagctctttt tcctcaaagc tcactactct tatcccaaaa
    17821 atcattttcc caattgtttg tcctgaattc agcttagtca aaagtacaaa ataggttaaa
    17881 taaatagcta atgacgctat accataaaaa gtaataatgg atttgtcaga ttccagtccc
    17941 atgattttgt aaaagagacc tatagtagca tgggtgatga tgctaataca aattaaatca
    18001 ataagaaaag caaaaaatcg taaccaaaat cccgcaaata cataattagg caaagtttca
    18061 atgccactct tcttattcat tttatagccc tccatagtaa tacattggtt ttggagtatc
    18121 agaaaatcca agtttttcaa aaactgaaat tacacgatct gatgtagttg cctttagccc
    18181 ttgccattca gcaatcttat tacctaacca agaagaagca aagccagttg tgtcattttt
    18241 atattctata accgtcgcat cagttaattt cttctctttt tttagggaat ctaatgcatc
    18301 ttcagagtaa ccaatttgat ccaccaaccc attttctact gcttggttac catcataaat
    18361 tcttccatcc gcaagttctt tcactgcatt agtatccata tttcttcctt ttgctacaac
    18421 attgacaaaa cgatcataag cactcatcac aaattcctgt ataactttat tctcttcctc
    18481 tgaaggtttg tgttgaggtg acatcatatc ttttaatgca ccacttttat acgtcgcatc
    18541 agttacgcca agtttttcta ataagccaga ataatttaaa ccagaaataa tgacaccaat
    18601 tgatccagtt gttgtttctt ccgtagcaaa tattttatct gaaccagcag aaatgtagta
    18661 ccctccactt gccgctgtat ttttaaatgc agtatagata ggaatatcta gctttttaat
    18721 ttttgccatt tctttagcaa tctcagcact ttcatatatt cctcccccag gtgaatttac
    18781 ttctaataac actcccttaa ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa
    18841 attctgatgg ttatattgtt ccctactaaa aagattgctt tcacctgtat ctgcaataac
    18901 tccattgacg gatagtttga caatcttttt atttgatgcg ccttcttcta acgtttcttc
    18961 tgtgatttcg ttagatccgt aaaaaatctt atttattcct gttagggttg tatttgatgc
    19021 ttcctcttta tcttttcctg gaattagcgc gacgataatg gatagtaaca ataatccgca
    19081 cgctactccc acagcaatcc atcttctttt attcatataa actcctccta tattcttagt
    19141 aaaaataatt attttttatt tagaaacaca tcgacaattt gttgccaata tgttttttct
    19201 cctacaatta aagacaactc tccagttaat ccataacgaa acggaaacga ttgagttgtt
    19261 tttaacgttc ctttcactgc ataaaaaaca cctttctcag tggcagtaga attttctgaa
    19321 atttcatcta aagttccgtc gatcgtaatc ggagccccac ctttttgatc caatttaaaa
    19381 tgaaccttca tcccttcttt aatataggta ctttcattcg caggtaaaag tgctgtaaat
    19441 ttcatttttg atttttcgtg gatcggataa atttctgcaa gcactgttcc tttggctagt
    19501 tctgcttgtc cctttgtttc ttcatttaaa tgaaccgttc cagatacagg agcttttagt
    19561 attccttttg aaagttgatc gtccaaactt ttgataactg actggttctt ctcttgtttt
    19621 tcatttagct ctgtaactgt ttgttttaca gttgttattg cttgttctag ctcttgcttt
    19681 cctttttccg tttgactaat tctgtcattc tcagaagtag ttggtttaac taatttagct
    19741 ttttctgact gatactgttc tacttctttt tttagttgct caatttgttc atttatgctt
    19801 gctgaaattg tcagtttcac ttgatttttt tgatcatcag aaacattatt tagttgttct
    19861 tgccaattct catagtttgc catcatttct tttgaaaaat cttttaatga ttgattatta
    19921 ctccaagcta cctgtacttg ttgccattct tgtaatttac tatctgaact ctcaatttgt
    19981 ttttctagtt gtctttttgt cttttcgtat acttctaatt gcttttgatc atttaattca
    20041 ctttgcttca aagcgtagcg gagtgattct ttttctgaca acatgctctt taattgatta
    20101 ctgtagccaa aactgtcatc cgttgaaaac aaattttttc ctttacttat actttcaata
    20161 aatagctgag ccatctttat ttgttgagta atacgttggt tttcttgttc tatctgagcc
    20221 ttttcattct ctattaaaga acaatccaat ctaaccaaca catcgtcctt atgaacaaat
    20281 tgattttctt ttaggaagtt ttccattatt ttagcttcta atggcacttg taacttctct
    20341 attttttcgg ggttcaattg agccgttgtc cgaataacaa cctctttttt agcaaaggtt
    20401 aaaaacaatc caataataca taaaaaaaga attacagggt ataacaccca acggtagaag
    20461 ctatgatgtt gtttagtata gatactagaa tgttcgaacc aattattatt ctgcataaat
    20521 tgtttccctc tcctagattt cccataattt ttgatagata ccatcggcaa aacgaagttc
    20581 atcgtgcgtt cctttttcta ccaaatgccc ttcatggaga actaaaattt gatcacatgc
    20641 ttttgctatt ggtaaatgat gcgcaataaa aataatcgtt ttttctttta aattgaggag
    20701 ataattcata attgcatgtt ctaacaaggt atctaatcca ctagttgctt catctaaaat
    20761 taatatgtcc ggatttttta ataaagcccg tgctaaggct aatcgttgtc gttgctctcc
    20821 tgaaagatta tttccacctt cttccaacaa tgtctcgtaa cgtaatggct gttgattaat
    20881 aaattcattt agttggacta tctcacaaac ttctattatt tgttcgaaac ttggtgggct
    20941 atctaaaccg aatataagat tctcaatgat tgttccacta aagaaaaatg actcttgagg
    21001 caaatatgtc actcgatcgc gtaatatctg aaaaggaatg tctaagtgat taatgtttcc
    21061 ataatggatc gatccttctg atgcttcata aaaacgaaca aataactttg caagagttga
    21121 ttttccagaa ccactaactc caactatagc cgttttactg tgcgaaggaa ttgtacatga
    21181 aatttcgttc aacgttgggg ccttcatgct atacgagaat gaaacgtttt ttacagtgat
    21241 atcttgttga aataattgtt cagaaagcat atattcaaaa gactggttcg atttttctga
    21301 atcaatcgcg aaaatttcat ttaaacgatt atttgcaact tgagccgttt gcattttcac
    21361 ttgtaaatta ataatgtttt gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt
    21421 gataagttga cctagactaa ttctattatc cataacaaaa taagaaccta gccataaaat
    21481 taatgcactg ctaattaatt gaatcaaatg tttaattcct tgttgaatat tatcaagcgt
    21541 aaccgtacga aatgattttt tcattaattt aacaaattca tggtctatac ggttatatac
    21601 tttctcttca ccgctatatg ctttaattgt ttctattcct ttaagacttt ctatgatact
    21661 agaatttaca gctgcaccgg ctgccatttc ttccctgttc gatttttcat agcttttcac
    21721 gaaagcgaat attgcaacta cataaaaagg caaagagcat agagtgatta aaaacaacgt
    21781 actattctga atagctaacg taaccccaac aagcaacacc ataccaatat ctaaaaatat
    21841 tgataacgta gcacttgcta aagcatcaat aatcttactg gcatcaagaa aacgagaaat
    21901 aatttcccct gattttctcg tagcaaaaaa attcattggt aaagttaaca catgtttgaa
    21961 atactgaagc ataattgcca tactcattcg ctgtcctaaa ataataagaa gataactacg
    22021 actatattca aacaataccc gaaataaata gacagcgatc aagccaatgg aaattatatt
    22081 taaagttgaa cgtgcttgat tagggatgaa aaaatcaaga ataccttgaa aataatatga
    22141 gcttcctatg ccaaaaaacg taatgaaaat cgatgctaaa acgatgtgga atactaatcc
    22201 tttttgtttc caaattatag gcaaaaaaga acttaatcca gctacttttt ctttaatagg
    22261 aatataagtt tctctaggca taggtaataa aagaatacct gtccattctt tttgaaacgc
    22321 ttcaatcgtt tttctatttt ttccattagc gggatctgca atccatagta cgtttccctt
    22381 cgttttataa attacaacgt agtgcatata gctgttatca accaatatat gtgcaattaa
    22441 tggtaagata agatctcttt ctttccagat ctcactatct gcttgaatag ccatacaatc
    22501 aaaagacaat ttttcaaatg tctttttcaa tccaaatgct gaagtaccct ctaaatcagt
    22561 cccagatagg tcccgtaatt tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat
    22621 cgaaaggcaa gcaacaccac aatctttttc atcttgttgc tgcacaaaag gaaatgattt
    22681 tatcacttat ctaacacaac tccttctaaa aaatttttat agaaaaattc tcaatatagc
    22741 tttaatacag tgaaaatatt gtctcataat tccacaaaaa ctttgaaaac agttcttgaa
    22801 cactaacttt ttcattcctg aatcaaattt attttggaaa ttttactatt caagaataaa
    22861 cttgataagt ttcaggaaga tttttactgt taatcaaata cgtatgttac agtagcaccg
    22921 taaaaaatat tatttaaaga agcgtgttta aacatgcaaa atgtaaagga actaagtatt
    22981 gtggaaatgc acaaactgtt ggtggaggca ataatgcttg gggaaaattg gggcaagttg
    23041 taggtggact taccacaggg gcagtaggtg gtgcaggttt aggaacagca atttgcggac
    23101 cagcttgcgg tgttgttgga gggttatatg gagctgtcgc tggaggtgct gcagctggct
    23161 gggatgctcg aaaaaaataa aaaaggagaa taagtagtat gcatattaaa aacacaaaaa
    23221 caacatttat attatcttca gaagagttga aaaatattca aggtgggagt gccgtgggag
    23281 ttttaggcac tactttttct ggggcaacag caggagtaaa actttgttca gccggaggac
    23341 cttatgcaat agctgcttgt ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt
    23401 ggaccggtgc gtaaacaatt aaaaaatatc aaacctcaag attggtttgt gtcgattacg
    23461 aacataggta tgttctttat cttttcaaaa gtttggaatg gacagaactt tttactaaag
    23521 cttcttgtgc tagtttttat aattatatta aatttttatc ttttattaaa gtcagataaa
    23581 aattctttgg aaatgtaatg gtagagatat ttttacaaaa ggacttgcag cgctgtagtt
    23641 ggtgctgcag gaggacctat ttcggccgtt acaggaggta ttggtaatgt acttgtcgga
    23701 tgtgcattaa agcctgttcc tgcaagttaa atttataaaa acaggaaagt aattatctca
    23761 aatcctatca agtacaatca taggattgca cttgacagga caaatttttt aactaaaaaa
    23821 tgaaaaacac tttctaaaaa ttagtagaat ttgccaaatc tttcgcatct tccaccatac
    23881 atttaatttt aattcccctt tatggtactt aataaaaaca agaaaagaac tattcatttg
    23941 gccctttttt ggttccagac catagaaata ttcctccagt aactaatatt gctgttatgc
    24001 acgtaactaa taacagctat aaaaattggt acaactttaa taggattcag aaaaatttgc
    24061 actagtgaag gaatctattt tattagaaaa atgcaatctc cctaaagaaa aaaccactaa
    24121 acagtgcttt ctttcaactt ctccttaatt ttttgtgctt tccctaaagt aaagtttctg
    24181 gccaactctg aatcaaaata tagtgaacgt tttttaaaat ctatttcttt aacgttaaag
    24241 atatttacta aacacgctcg actaattctt gttaacatag gatatttctt ttcaagctca
    24301 ctaattcttc cataaaattc gtactgccca tctttagtac acaatgataa tctatgtggt
    24361 aattttgaag cttctacaaa ataaatgtca tttatatcaa aagtaaatgt ttgagaacct
    24421 atggaaaaaa caaatgcttg attcgtaacc tgttttgttg catctatcct ttcttgcgct
    24481 aataacaata actcaacaat ctcgtttcga tactcatcta gtgtttgatc ttttgttaca
    24541 aatcctaacg tttcaactct tctttttatt gttacgggta acatctcatc atgagtagtt
    24601 gtaaaaatga tttttgcttg aacatcatat tttctgatta cttctgctaa ttctataccg
    24661 ttaacttcat gatttaaatc aatatctaag aaatatattc cttgctttgg tcggaattgt
    24721 ttgaggtatt ttttaacttc taacggactt tgtgttttca atactatctt aaatacttta
    24781 tcgtgaaaa
//
LOCUS       DACDKJ010000037        24730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDKJ010000037 DACDKJ010000000
VERSION     DACDKJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.037"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(30..1061)
                     /gene="queA"
                     /locus_tag="IV328_001690"
     CDS             complement(30..1061)
                     /gene="queA"
                     /locus_tag="IV328_001690"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8937827.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1254..2429
                     /locus_tag="IV328_001691"
     CDS             1254..2429
                     /locus_tag="IV328_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8937828.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2433..3074
                     /locus_tag="IV328_001692"
     CDS             2433..3074
                     /locus_tag="IV328_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937829.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3071..3988
                     /locus_tag="IV328_001693"
     CDS             3071..3988
                     /locus_tag="IV328_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8937830.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            3985..4656
                     /locus_tag="IV328_001694"
     CDS             3985..4656
                     /locus_tag="IV328_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937831.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5197..5685)
                     /locus_tag="IV328_001695"
     CDS             complement(5197..5685)
                     /locus_tag="IV328_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8937832.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5886..6152)
                     /locus_tag="IV328_001696"
     CDS             complement(5886..6152)
                     /locus_tag="IV328_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8937833.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6152..6415)
                     /locus_tag="IV328_001697"
     CDS             complement(6152..6415)
                     /locus_tag="IV328_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8937834.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(6408..6680)
                     /locus_tag="IV328_001698"
     CDS             complement(6408..6680)
                     /locus_tag="IV328_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937835.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            6891..7076
                     /locus_tag="IV328_001699"
     CDS             6891..7076
                     /locus_tag="IV328_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8937836.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7218..9041)
                     /locus_tag="IV328_001700"
     CDS             complement(7218..9041)
                     /locus_tag="IV328_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8937837.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9639..12344)
                     /locus_tag="IV328_001701"
     CDS             complement(9639..12344)
                     /locus_tag="IV328_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8937838.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12650..13597)
                     /locus_tag="IV328_001702"
     CDS             complement(12650..13597)
                     /locus_tag="IV328_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8937839.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13787..14056
                     /gene="rpsN"
                     /locus_tag="IV328_001703"
     CDS             13787..14056
                     /gene="rpsN"
                     /locus_tag="IV328_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8937840.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14232..14594)
                     /locus_tag="IV328_001704"
     CDS             complement(14232..14594)
                     /locus_tag="IV328_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8937841.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14605..15726)
                     /locus_tag="IV328_001705"
     CDS             complement(14605..15726)
                     /locus_tag="IV328_001705"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8937842.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15742..16092)
                     /locus_tag="IV328_001706"
     CDS             complement(15742..16092)
                     /locus_tag="IV328_001706"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8937843.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16222..17733)
                     /locus_tag="IV328_001707"
     CDS             complement(16222..17733)
                     /locus_tag="IV328_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8937844.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18055..19407)
                     /locus_tag="IV328_001708"
     CDS             complement(18055..19407)
                     /locus_tag="IV328_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8937845.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19560..20636)
                     /locus_tag="IV328_001709"
     CDS             complement(19560..20636)
                     /locus_tag="IV328_001709"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8937846.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20832..22577
                     /locus_tag="IV328_001710"
     CDS             20832..22577
                     /locus_tag="IV328_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8937847.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22857..23294)
                     /locus_tag="IV328_001711"
     CDS             complement(22857..23294)
                     /locus_tag="IV328_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8937848.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23278..24651)
                     /locus_tag="IV328_001712"
     CDS             complement(23278..24651)
                     /locus_tag="IV328_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937849.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
BASE COUNT         7052 a         5235 c         4631 g         7812 t
ORIGIN      
        1 ttattcaaaa ctagcggctt ttcataacct tatttcacga acatcgcatc tccaaaacta
       61 aagaaacgat agcgctcttc aatcgcatgc tggtaagcag ataaagtcaa ttctcttcct
      121 gcaaaggcac taactaacat gactaaagtc gattttggca aatggaaatt agttgagaaa
      181 gcttctacca ctttgaattc atagccaggc gtaatgaaaa tatcagtcca tccgctgtct
      241 gctttgattt ctccattaaa tttcgttcca atcgtttcta atgttcgaat tgaagttgtt
      301 ccgacagcca cgattttccc gccattttta cgtacttggt tcaatcgctc agccgcttct
      361 tccgtcaaac ggtagaattc actatgcata tggtgttctt caattttatc tacgctgaca
      421 ggtcggaatg tacctaaccc tacatgtagt gtcagataga ctaattcaac accctttgct
      481 tcgatctcat caagtaattc tttcgtaaaa tgcaatccag ctgttggcgc tgcagcagaa
      541 ccattttctt tcgcatagac agtttgatag cgatctgggt cttccagacg ttctttgata
      601 tatggaggca atggcatttc tcccaatgat tcaagaatct ctagaaaaat tccttcatac
      661 tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt tcactactgc ttgcaatctg
      721 ccatcaccaa aagagattct tgtcccaact ttggcacgtt tcgcaggttt gatcaatgtc
      781 tcccacgtat ctccttgtgt attcgttaga agaaggactt ctaaatgcgc accagtatct
      841 ggtttttctc catataaacg agcaggcaga actcgtgtat tattcataac tagcgcatca
      901 ccaggctgta actcttcaat gatttcatgg aaatgtttat cttccatctc tccagttttc
      961 cgatccaaaa caagtaaacg agaatgatcg cggtctttta atggtgtttg tgcgattaat
     1021 tcttccggca agtcaaaatc aaaatcttct gttgttaaca tatcgtcact cactttctat
     1081 catgtgcttc atcattctat catgtttttc gttctgttgg caaaatcagc actttttcac
     1141 ttctattttt tgttttccta aataaaaaaa ctaccctatt tgtatttttt cattgtttta
     1201 ggcatactag taatagatag ttcttcattg aaaatagaaa aagaggtggc accatgattg
     1261 aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt cgctgtagag gacgtcaatc
     1321 tttcatttga aaagggtgag ttcatttgtt ttatcggtac gagcggaagc ggaaaaacga
     1381 cgacgatgcg gatgatcaac cgaatgactg atccaacaca agggaaaatt ttgattgatg
     1441 gaaaatccat ccaagaaatt gatccagtgg aattgcgacg tcagatcggc tatgttatcc
     1501 aaagtattgg attgatgcct cacatgacga tccgtgaaaa tatcactctt gtccaaaaat
     1561 tgttgaaagt cagtcaagaa gaacggaata aaacagctga aaaaatgatt gatcttgtgg
     1621 aattgccccg tgagatgtta gatcgctacc cacatgaatt atctggtggt cagcagcagc
     1681 gtattggagt cgttcgtgcc cttgcagcga atcaagatat catcttgatg gacgagccat
     1741 tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga ccttgtcaaa gatttacaag
     1801 agcgtttggg aaaaacgatc gtatttgtaa cccatgacat ggatgaagcg attaaactag
     1861 caagccgtat cgccatcatg agtgaaggcc gtgtgatcca atttgataca ccagaaaata
     1921 ttttacgtca tcctgcaaac gatttcgtag aagagttgat tggagaagat cgcctactac
     1981 aagccaagcc aaatgcgaca agagtcggcg aagttatgct aaatacagca atcacgatta
     2041 caccggaaaa atcattacaa gaagcaatca aactgatgag ggaaaaacgt gtagatacgt
     2101 tgttagttac cgataattcg aatgtattaa aaggatttat cgatgtagaa acgttgaata
     2161 aaaaacgagg aaaagtatcc agtgtcggcg acattttgaa taaacatgtt ttttacgtaa
     2221 aagaatccgc ctatcttcgt gataccttgc aacgcatttt aaaacgcggg ctgaaatacg
     2281 tgccggtcgt tgatgaacag aacaaagtcg taggtatcct gactcgtgca tcattagtgg
     2341 atatcgtcta cgatgtgatt tggggagaag atcccgcaac tactgatgtt gcagagtcag
     2401 ctactcctga agaatctaag gaggtctgac ctatgcagca gttttttcat cagcacggtt
     2461 cagaaattct ctcaaaaggt tttgaacatc tttatatttc aggacttgct ttgatcattg
     2521 gtatcatcat tgctgtaccg cttggtgtct tactgacacg ttttccaaaa gctgccacga
     2581 ttgtgattgg cttaaccagt gccttgcaga caatcccttc tcttgcttta ttggcattga
     2641 tgatcccgct atttggcgta ggtaaattac ctgcgattat cgcactcttt atttattcat
     2701 tattgccgat tttacgtaat acgtacattg ggatgaaaaa tgtcgattgg aattatcggg
     2761 atgtggcaaa aggcatgggg atgactgagt tccaatccat tttttccgtt gaattgccca
     2821 ttgctatgcc aacgatcatg gctggtattc gtttagctgc cgtttacgtg attgcatggg
     2881 ctacacttgc ctcttatatc ggcgctggtg gtttaggcga tttgattttt agtggactga
     2941 ataattacca accagatctc attttggcag gtacgatccc tgtaacaatt ttagcactat
     3001 tggctgattg gcttctaggt ctcttggaac accggctgac accgatcgca cttagagagg
     3061 agaatgaaga atgaaacgta tactaaaaat gaccatggtt cttttctctc ttcttatact
     3121 tgcgagttgt tccttccctg gtcttgccag caatacagat gaagacacca tttcgatcac
     3181 aggtggaatc acttcagaag cacagatctt agcaagtcta gttgccggaa tggttgaaca
     3241 ttatacagat aaaaataccg caatcatcaa caatctggct actacaacta tcaaccatca
     3301 agcgatgatg aacggagatg cttcaatttc agcggctcgc tacacaggaa cagatttaac
     3361 gactacattg aaccttcctc ctgaaaaaga tccgaaaaaa gcgtttgcta ccgtgaaaga
     3421 tgaatttgag aaaagatacg gtcaaacgtg gttcccgtcc tatagttttg aaaatacgta
     3481 tgtctttctt gttcgaaaag acaccgcaca aaaataccat ctatctaaag tcagcgatct
     3541 gaaaaatgta gcggatgaat tagtagctgg tgtagatact tcttggatca atcgtaaagg
     3601 agacggttat gacggcttcc aagaaactta tggcttctcc ttcaattcga tattacctat
     3661 gcagattgga cttgtttatg acgcagtcga agcaggaaaa atggatattg ttcttggcta
     3721 ctctacggat ggacggatcg ccagttatga tctagtcatg ttggaagatg atcgtcaatt
     3781 cttcccacca tatgatgctg ctccagttgt agacaacaag atattagctg atacgcctca
     3841 tttaaaagaa gccttaaata agttaggtca taccatcagt acggaaaaaa tgcaggaatt
     3901 gaactacgaa gcagataata atctagtgga accatcagta gtagcacaac gtttcttaca
     3961 agaaaaccac tattttgaag ggaagtgaag caattggata aaatgaatgt ctttcaacaa
     4021 tttttctatt atttccagga aaatggctcc tatatctttg cacaatttat ccgtcacttc
     4081 ctgatttcta tctatggtgt cctctttgca gcagttgtcg ggattccggt tgggatcatg
     4141 atttctagga gacgtaaatt agcaaactgg gtgattcgtt tggcgaatat catccagacg
     4201 atcccttctc tagccatgat ctccatttta atcattggat taggcctggg agtcaatgtc
     4261 gtgattgtta ctgtattttt gtattccctc ttaccgatca tcaagaatac gtataccggg
     4321 atgatccaag tagacaaaaa catccttgat gtcggcaaag gcatggggat gactgctaga
     4381 caacgactat tcatggtaga gttaccactc tctgtttctg ttatcatggc aggcataaga
     4441 aacgcgttag ttgttgccat cgggataacc gcgatcggtg cattcgtcgg tgctggtggt
     4501 cttggagata tcattatccg aggaaccaat gcaacagagg gcacatcgat tattttagca
     4561 ggtgcattgc caactgcatt aatggcgatc atcacggact ggcttttagg tattttggaa
     4621 agacggctag atccagccag ccgtacatca cgctgatgtt attgtaaaaa acaaaagctt
     4681 ctgcgaccat ttgttggtcg caaaggcttt tttagttctg tcatttttaa ggcaataatc
     4741 cactctgatt cttccctcct tatgggtaaa gagttgcttt tcttagctat ttatccattc
     4801 cgtatactcc aacttcatac tgccaacggg ttcatatctg catccaccga ctaagttttt
     4861 ttcttatccg ctaaggcatt ttgccaatcc attttcccat cactcgtcat ggaattcata
     4921 ccacatgatc tctaaatact cgtctgcttg ttctttgaat tgggcatcaa ttcttacatc
     4981 ctttggcgtg gctttcattt aagccttttc catcagtcct cgattgttcg atgccttcag
     5041 ctgcatcatc aaaactggtg actgccgtat cggtaacttc gatcatcttt tatacaatcg
     5101 ggaacttgcc atcctcatcc ttggctaaaa acatatctag ccattccagc ttctcgccat
     5161 gggaggtttg cggttcctaa aaccggtaca cgattcttat ggactgcatc cgtcgcaact
     5221 agacttggcg atacgatgat ccttcaccag aagagcaccc ccgtaaacca attgatcgat
     5281 atattgccaa taagaaaaga cattggcgta aaagatatcg atccctcgtg atgcattgcc
     5341 gttgatactg ctattcatgg ccgaaagcgc cacgacttta acttcttccg tttgagtgac
     5401 attcatgttc ggcaatgtcg ctttggatac ccgctctgct aaaggaacga cactggtatt
     5461 gtatttcgta tctgtatctt ttccaaaaga ccatgccaac gaatcctctg ggaaccaata
     5521 agacgactcc ggttgattgt ccattgcttt tttcaacata cttttggctg tttctcgata
     5581 aacgacattt ccaattgtct tcgcctcact ttggcgataa tcgatggtcg ttgccactgc
     5641 caacactaac aacgatgctc caattttact gattttcctt gtcacacttc ttcctccttg
     5701 ttttacataa acccgtcaac attccattcc taaactcaaa caatgaccct tcaaaggtgg
     5761 agatatagga acaaattaaa gaaatcatgc aataaatcca agaaggttgg ttttagaaca
     5821 aaaaaagagt ctcctttttg gggagactct ttgttaacta tctaaaaagg ttggctcttc
     5881 tcacttcact cataatgtgt ccaggcagaa taaatttcta caactttatt ttcctcatca
     5941 actttgtaaa ccactcgatg ctgtctattc agcctgcgtg agtatctgcc ttcatgtgta
     6001 ggtctcagct tttcaaaaga ctgcgtgggc atatatggat cttccttcaa tgtctgaatc
     6061 acttcttcaa attgtttctt taaattcgtt tgcttgattt ttcttaaatc cactttcgca
     6121 gagtttttga ttgcgactgt atagttactc atcaaagagc ttcccaatca atatcatcga
     6181 tattcgtaaa gctactgtcg tctttttcgc ggtctcgtac tttatctaaa gtacctgttt
     6241 gctcaagaaa aagggtttct tgaatcgctc tccagtcttc cagcccaatc aatacagcat
     6301 tgtttccact acgatcacta ataatctcga tttcagtatg attttcattt acctctttca
     6361 ataactgata aaaattttta cgagcagtgc taggattcat cgttgtatta gccattgtct
     6421 ctcctccttt ttctgatata attatagcgt acttgattaa gtacgtcaaa aaaatacaat
     6481 aaagcactta aattcagcaa acttttcctc ttttttacat agagaaaaag accccttttt
     6541 agggaggatc tttgtccacg ctcacttctt actcctttac gactagcggt agccaaacgt
     6601 aaagtaattg gtaacaacgt atcatcttta gcaaaggtcg aatccaaagc ctgtgagtct
     6661 atgcaacaac accaatacac gaaggttcct cgttgcagtg tgacttgatt gatatcttga
     6721 ataacctgag gatacgcacg aaagatttgt aacggttatt caaaactgat tttggccgtc
     6781 aagtcttcta ctgcctcaaa aatgattgcc tctttcgtct cctgtcattc atgttcacta
     6841 tcgatttgat acttgcttag accgttgggt ttgtgaatca ccaattaaaa atggcactga
     6901 gttatggtaa caaaaaatgg tcacttgacg aggctgagat tcctacttat gaagacacat
     6961 ggcacttaag agtcgaggta aaggaagaag ctgaaaatat aggaggctgt acattatttg
     7021 gcaaaggatt cggaaattat gatcaagaca tcaaaatcaa aatgttttac agctaaatag
     7081 gaagaataat aacatttcat acgctgttcc catctttaat ggcttataga gggcaccttt
     7141 agctgagaat aagacaaaac agaccctcta gcttcaatta agagctagag ggtctgtttt
     7201 tgattttaaa acttggttta tttcttcaaa tggtagctat aggtgctgac aacgatattt
     7261 tcacgccaag acaatcgcaa tcttaaccgc aaacttgttt ggttatggag aatattctgc
     7321 cagcgtctgt ttggtacaaa ttgaggaatc aacacagtag ttgtatagtt ttgtttcgct
     7381 gcatttttac ttacgatatc tacataacgg ataattggat tttcaatcga gcgataagaa
     7441 gagtggacga ttgaaaaacg gacatctggg aaatgctttt taaattctgc ttcgatttct
     7501 ttttcttttt cgacatcttc gtctagcgaa acatgcatcg caacgacata atcaccgatc
     7561 gaacgtgcgt aattcaacgc accgatattt acccgtgtca cattcccgac taatacaaga
     7621 acggtattcc cgtcataatc atgtagtttt gcatcatttt ctaaacgaag ctgttctgct
     7681 acttttttat aatgatcatg gatcttataa aaaatgaaca taacgattgg cataatgatg
     7741 aagaaaggcc aaatgtcccc taaccgatag acaaacaaaa tcgcaatgat tgcatatgag
     7801 atgaacgcac ccgtaatatt ggcaatcgat ttgcttaacc atctttttcc ttcttttttc
     7861 catttgacga ccatccctgt ttgagataaa gcaaatggaa tgaacacgcc gatagagtat
     7921 aacggaatca agcgttctgt tgagccatgg aaaataaaca gcaatacgat cgaacctagc
     7981 gctagtgtga taatcccatt ggaatagcct agtcgatccc cacgatcttg atacatgtga
     8041 ggcatgaatt tatcttttgc cagattgtat gcaagaactg gaaaagcaga aaaaccggtg
     8101 ttagcagcaa ctgctaaaat cagcgctgta gcaaattgaa ggacgtaata caaaatacca
     8161 tgtccaaaga cagctttacc aatttgagac aaaacagtga cttctttttc aggaacgatc
     8221 ccataccaat agttgataaa tgtgatcccc acgaagaaga aacctaagat caatgccatc
     8281 atcgtcaatg tagcagcggc attttttgct cttggcttct taaagaatgg tacagcatta
     8341 ctaatcgcct ccaccccagt taaagaggaa gaaccagaag aaaatgctcg taaaatcaaa
     8401 gcgatagaaa cacccggcac agccgctcct ggaagtgcag tagcttgcaa tggcacagca
     8461 cctgtcacga tattatataa accaacaacg atcagtaacg agatcacaag gataaatgtg
     8521 tacaccggaa ataataggaa cgatgcagat tccctcagcc cacgaagatt cagcatcatc
     8581 agtagcaaaa cgattgtcac agaaatagcg acttgatgac cgtagagtgc tggcaccgct
     8641 gatgtgatcg cttcagcacc tgctgagaca gatactgcta ctgtcaacat gtagtcgatc
     8701 aataaagagc caccggaaat caagccagca ttctttccaa gattctcact gctgacgaca
     8761 tatgctcccc cgccatgagg ataagcatgg atgatttgac gatatgatag cgttaatgaa
     8821 atcaataaaa taataacaaa agcggcaatg ggtagcgaat accagatggc tgcagcagac
     8881 aaagcgacta aaacgacaac gatttgttcc gtaccatatg caatagatga taacgcatca
     8941 gacgacaata atgccaaagc agcaaatcgg gttaatttgt gctcatcatt ttcagcagat
     9001 tttaaaggct tacctactaa caaccttttt aagtaatcca atttctaaac tcctttacac
     9061 acaattttac ttcaagtttt atagtcgaaa atttatagta aacaagatgt tatagtacgc
     9121 tcactgacct taaaagtcaa taaactttaa gaaaataatt ggaaaaaaac agaaaaatat
     9181 caagcgcttt ttcaaacgct ttaatacagc aaaaatctaa cattttttta tgggcttttt
     9241 ctttgaattc tataataaaa ggatagaact tgacttagaa aatcaagttc tatccttttt
     9301 gatatcttaa cttttggaag agagatgtaa ggctgctttc tacccatcat tcttactact
     9361 cgaagcatag cgcagttgtc acaacctctt gataaacggt gtttaaaagc ttgcatctgc
     9421 ggtaataagc ccaaccaaat taaaaacata agaagctgtt tttatttggt cgttcttatt
     9481 acttgatgca gagcagcttt tttacaacct cttggtacac ggtgttcaat cagctgaccc
     9541 tcggtattag ctcaaaaaaa cggcaaaata tgaggaacta tttttccatt tttttctcta
     9601 atactcaggt caaaacactg atttcacaac ctcttttttt aatgaaaaat atggctctca
     9661 tgcgccgctt ctttgaattt cgtcattttt tcttcgatga acggatagat tgccgttcct
     9721 acgattccaa agacaacaaa caaaccgatc atgatccaga ttgctttcgt agcatttggc
     9781 caataaattc ctcctgcaga ttcccgtaac aagtttaccg catgagtaaa tggcaagaac
     9841 ggattgatca tttggaagaa tttccctgag acttggatcg gatagttccc gcctccgcca
     9901 gagatcgaca gtactaagat gataatagcg atccctttac cgacattatc gaataatgca
     9961 gccaagacat agacgatcat catgaacgct aaggcgatca agacagcaaa tgacacgctg
    10021 tagaccggat tttggacatc tactcctagt aagaaaatat tccctaatgt aacgatcaag
    10081 ctttgcgcaa tcgccattgt caaaaatgtc agcatacgag caacgaattg ttctcgtttc
    10141 gtaaaattgt ttcggtcttt ctttcctaaa tagtattctg tcgtagcaac acttgaaaga
    10201 agaacagcgc cgacccacag acatagtgca gtataaaacg gggtgctagc tgaaccgtta
    10261 tttgcgattg gataaagcgc attggtttgt agctcaacag gttgtgtaaa gaagtcactt
    10321 tcttttgttg cgtctagttt caataatttg atgacttcgc ctagatcgac ttcttgttcg
    10381 ccttttcgga tcgcgtctgc tgctttttgg ataccagctt tgatatttgg ccagtcattg
    10441 ttgatcaagt ccgcagcagc gttcacagct gtttcgactt ctggcatttt ttcatttact
    10501 gttgtcaatg taccggtgat ttcatttttt agtgctggcc aatcattttg gataaaatca
    10561 gcggccaatc ctaatttttg ctgaatgatc ggtaattcat tgttgtatag gtctgcccct
    10621 tcattgatgc cattgacgat ggtatccata tttccattca gcattgtatt ggcatcatgg
    10681 atttcttgtc caattgcagg catttctttt tgatattttt ctaaaattgc cactgcattc
    10741 gttacagttg ctttcgttga gctgagaagc gaatcaaagt cgatctggtc cgctttgctc
    10801 agtgcgcctt gtgcagtccc aatcgtatcg atgacttggt ctagtagaga agtgacagtg
    10861 gaactgattt gacctgtatt gataccagca atgatgccat tgatattatt aacaacggca
    10921 tcaatttgat ctagcaattc atcaatagcc ttcgtgtctt cattttgaac agctgtgttc
    10981 aaagcagata ggcggttttg cagattgctt agcaagggtt ttaattgatt tagctgatca
    11041 ataattggct gtaatttcgt atttccagca cttgtttgca atttagtcag gaactcgacc
    11101 agtttatcga ttgcattttg ttgttgagat attccgtcaa taaagccgtt caatacattg
    11161 ctgatgtttt tcttttcatc atcagtcagc ttaccatctt caatagctgt tcttacagta
    11221 tctattaacg aacctgtcgt tgttgccact tgtccaattg acttcaatgt aacatctaca
    11281 ctgtttgtga tgcttggtat cgcatctttc agctgagagg cacctgattt tgtttggttt
    11341 gctagatcac ttgcctgatt ttctaaattt ttcacatctg gcaagatatc ttgtacttgt
    11401 tggatgatcg tcaagccttg ttttgcttca tcgattcctt ggttcattgt ctcttggatc
    11461 gaagcaaaat cgccgtcgat ctctgctagt tgcttaccgg cattttggat ctctgggatt
    11521 ttttcttgca aatcaaggat gatttttgct tgatctttca actcaggcat tttgtcattc
    11581 aatgcaacga ctttttcacc catctcatct actttaggga tgtaatccac gaattcattt
    11641 gctttattca gtttttcctt gatttcaggt agttgacttt gtaattcgag gacttgtttt
    11701 gtgtagccgt cgatcgtgtc caaattttca tctgttgata aaatcatatc tttaacttta
    11761 ttgatactta ctaggttcgt atcgatgtcg taacctatct cattgaatac tttcaataaa
    11821 gtcgaactgg ctgttttgat aaattcattt gtgatttgcg attggatcga acttgctcct
    11881 ttatcggtga ttttcggtgc aatcgcattg attttttcat ttaccgtata ttcgattttt
    11941 ggtttcttga tatcaccact agtaaaactc agcagatctt ctgagaaatc ctttggcaag
    12001 taaatacctg cgtaatactt tccggaacgg actccgtctt ctaattgttc ttttgaatca
    12061 acgaattgcc atcctaattg tttattttta tgaagcgatt cgatgacttg ctccccaatg
    12121 gccacttctt taccttgaaa ctctgctggc ttgtctgcac tgtaaacagc aattggcagc
    12181 tctccagtat ttccatatgg atcccataat gctttgatgt taaaccacgc atatagcgaa
    12241 gggatgatca tgatcgcgat gatcagaagt gttgcaatcg ggtttttgaa aatccgtttc
    12301 cagtcaagca aatataatcg aagcgtattt ttgattgact gcatagttct cccccttctt
    12361 cttttttcag ctgaattact ttaccatgta tttcatactt ttttcaatcg gcttttttca
    12421 aaaaagtcga atttttggac atatcacggc aattgtctca ttgtaatctc ttacatattg
    12481 agaaataatt aaggattaca gcctttactt tatcatgaaa taaaagggaa agataatttt
    12541 atgccaaagt ttttatctat tatacgtata cgcaaaaaag ctaaaacatt ttttgtcttt
    12601 gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct tatttttcga
    12661 atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt gcttggatga
    12721 catgcttaag tgaagcagac agattcattg aaataaaaac atgaaaggct cctttttggt
    12781 aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt ggaacaaacg
    12841 attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca tttaatgcaa
    12901 catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata cttcctttcg
    12961 tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt gttttatcat
    13021 aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta ctggatgcac
    13081 tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac ccgaccgcac
    13141 ctgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa tggttgatta
    13201 gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa ctcattttat
    13261 ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa gtcccgccga
    13321 ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa ccaatctctg
    13381 tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt cgtatctctt
    13441 tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct tttatttcta
    13501 cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca aaaacatctt
    13561 gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc ctttttcttt
    13621 aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt ggcgcatgat
    13681 tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc taaaagcgta
    13741 aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg ctaaaaaatc
    13801 aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg ctttacgcca
    13861 ggaattaaaa gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag atgctcgtcc
    13921 agaacgattg aaaaatcgtg atttaatcga tggacgccct cgcggataca tgcgtaaatt
    13981 cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc caggagtaaa
    14041 aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg catggaccac
    14101 tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg gtactagaag
    14161 cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc tgcatggtta
    14221 cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact aattttcaat
    14281 gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc tttcaatctt
    14341 gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc tattcctgtt
    14401 ccagtagctt tgatcccaat gtgagtcgga agcttaggtt tagccatttt tgccgttatg
    14461 gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat aaccaaaact
    14521 gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa ataaatatct
    14581 cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc gctgtcctaa
    14641 tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct gcatagtgat
    14701 ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa attcttgagg
    14761 caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt agttcccttc
    14821 taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac cgatcggtat
    14881 cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga ttccttcacc
    14941 tgctgggagt tttttgactt gaatcaattc agaaaccagc tcaagtgctg gctgcaatgg
    15001 ataaacttcc ggtagtgcgt gcccagaggg attaagtccg tacattgcta ctccataacg
    15061 aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc tgacatgtac
    15121 atatcgagga agttcgggaa gttttttcag cacttctata aagcgttctt tttgcagatt
    15181 ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac cttcccattc
    15241 taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag gtgtacaaaa
    15301 tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg tctctagctt
    15361 acttttctct aaaacagcag ctgctgcttg caaccattcc cgtgtacctg cagttactga
    15421 taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca agataaggat
    15481 gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg aaacacaaaa
    15541 gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat gaccgtatcc
    15601 gttcgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa cttcatttgc
    15661 tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt gccaagcgac
    15721 aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag ctgtttcatt
    15781 tgtatgagta atactgaccc atacattgcc ttcatgaggt gaacgcgtca caacaggctg
    15841 accattttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga tcccagttcc
    15901 ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt ctacttggcg
    15961 atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc gtgcaatgaa
    16021 ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat cgatcccaat
    16081 tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat tctaccaaat
    16141 tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt ctcttaaaaa
    16201 agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc cgcgtttttt
    16261 atcattgtga cgtttaccgc catctttttg gctgttatta tttttgctat aaccgccttt
    16321 tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc cgccaccacg
    16381 tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg gtgtgatctt
    16441 cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg caactaaatc
    16501 ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtatttat ctagaccatt
    16561 ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt tgaatgcttc
    16621 tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt caatcacatg
    16681 caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt ttccggcacg
    16741 gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat catagttgta
    16801 cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg ctacaaggat
    16861 atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt gagacaaatc
    16921 gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca attcgtctac
    16981 acggcgtttt gtacgtccga agacgattgt taattcaggt gtttgaacat caaacaagcg
    17041 agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat cgatcaagtc
    17101 agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga atttaacgcc
    17161 aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc tttgatcagg
    17221 aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca tttcatctgc
    17281 ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt taatgtggtc
    17341 aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc cacggatctg
    17401 acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt cgcgccctaa
    17461 acgatataat tcttcttgtg tttggatagc taattcacgt gttggtgcga taactaaacc
    17521 ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa atgcagctgt
    17581 tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg ctagtggaat
    17641 cgtttctgct tggataggcg ttgcttcttc aaaaccagcg cgttcgactg atttcaacaa
    17701 ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg gttgttaact
    17761 gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat tgtttctttt
    17821 tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac cgaaaaatca
    17881 ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt atcattatta
    17941 tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc tcttcatctt
    18001 tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag atttttattt
    18061 catttgctga agttgttcta ccacttcaaa taagcccatg ctgttgctgc cttttagcat
    18121 aatactatct gttgcttcaa gattttgttt gatagcatca atcaatttat tcttttcttt
    18181 ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt ttagtgcatg
    18241 catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat gttcactcat
    18301 ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta acacagcgat
    18361 tctgcgtcct tttgttggca gatttttaaa agtatctaat accagtccca tggctgttgg
    18421 atttgcattg tagacatcgc tcaaaatagc tgcaccattt cctgccgtta gccattcggt
    18481 tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta tggacagtcc
    18541 aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac ttccaggcag
    18601 cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc cttttgctgt
    18661 cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt gtactaatgt
    18721 tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc cgtctgatac
    18781 aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc caagattttc
    18841 tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca taagtgaaag
    18901 ttccgtgatt tcattggcgt gatccatgcc catttccaaa acgatctttt ctgtatcttg
    18961 gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc cttgcgtctt
    19021 atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg tggttttacc
    19081 attgcttcct gtaattgcaa cgacttctgc gcctactttt tccaagtaat aggttgccaa
    19141 ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatttt ttggagcttg
    19201 ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt gctggataaa
    19261 ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg ttatcttccg
    19321 gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc ccacggcttg
    19381 tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt gtgaaaaaag
    19441 cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt ctctattcgt
    19501 atttagaagc tagaatgaac gcggttacgc aatcactcca gcctcttctt tatttctctt
    19561 tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc taactggatc
    19621 agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag tgggtacata
    19681 ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc atttttattt
    19741 gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta agctaacttc
    19801 gcgtactctt gcgctttttg ataaacttct tctggcactt cggctggaat ctgcatttcg
    19861 attttattat tgatatattt tgcttcataa tcatagaatg ctacgtcttt tacgacttca
    19921 ccaggcaaag tcgtccgaac gtcttcattt cctaatacag caacttcgat ttcgcgcgct
    19981 tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc taaagcattt
    20041 tgcagctctt ctcggttttc tgcttttgta atgccgacac tagaacccat attcgccggt
    20101 ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt tttaggattt
    20161 tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc agcagcttgt
    20221 aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac gcctgcgccg
    20281 acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc cccgtttggc
    20341 ccatgtaaaa ctggaaaaac gatggctcct tcttctttga tttcgcccgg attgatcact
    20401 tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg caagacatct
    20461 ttgctggcag gtttttctgt tagtaatgga cctttgaccc attgtccttc ttttgtaata
    20521 aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga aaatgcggaa
    20581 agaatggaca cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga
    20641 ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc acaaaacaaa
    20701 aataaacgaa taacttattt ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt
    20761 tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga taaagaaaag
    20821 gagggatgat gatggcgcaa acaaatccac gcagccgctt ggcagaaatc attcgagtgt
    20881 tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa
    20941 aagcctttga agaactaggc cccaccttca tcaagatagg acagatgctt tctgttcgag
    21001 aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat agtgtaccta
    21061 gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt tcattatcag
    21121 atatattcga tgatttttca aaatcgcctt ttgcctctgc ttctatgggg caggctcatc
    21181 gtgccaagct gaagaacggt gacgcggtag tcgttaagat ccaacaccct aacatcgccg
    21241 aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc aaatacattc
    21301 ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt tccttgatca
    21361 atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa aagaacaatg
    21421 gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg
    21481 tgatggaaga aatgtctggt aaaaacttga atcatttaat gaacgccgaa aacaaaactg
    21541 aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca aagcttttag
    21601 ttgagaactt tatgaaacag gtgtttgatg atggtttttt ccatgctgat cctcatccag
    21661 ggaatctgct attccatgta ctaacaaaag aagaacagac acaagctagc cgaaaaactg
    21721 aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact tctgcagaag
    21781 accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg catctttctg
    21841 caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa gatgcttatc
    21901 gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt
    21961 tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt gatgaaatca
    22021 acttgcaaga agtattcaca catgtcgtga cgatttgtca tcaaaataat ctgcagttcg
    22081 atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc
    22141 tcgatcctga agtttccctc atggaagtcg cttcgccttt tgctcaacat tattttctca
    22201 ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg gaagggatga
    22261 aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg acttcgggac
    22321 aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg atcgaaagta
    22381 tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg tcctccctgc
    22441 ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg atatttacgt
    22501 atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc caaatgtata
    22561 agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag aaataagaag
    22621 gggttttaca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg
    22681 ctacttctga aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc cagtaataag
    22741 aacaatcaaa aagggaacat ggaacacatt tatcctgttt ccctgttccc tttttgatgt
    22801 attcgagcat aaataaaata ccttcgttac ctactatctt ctgtacttgg taaaccttag
    22861 tcaattgcct tttttaacgc ttgacgaaaa gacatatgat ctttctccgt aaagggtttt
    22921 ggaccctttg tccttatgcc agcttttctt aactttccac tatgataacg ttgttctaga
    22981 agcgtgccga tattcgcttc gtcaaattct tttccagagt gatggaacac tcgcacaccc
    23041 ttcggcaata caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc attcttctta
    23101 accataccga cgattttata atccgcacta tcagctccgc ggtctacgta gacgatttgg
    23161 acatgattag cgtattcttt ggtcgtataa tggtccacac tggtcacgat aaccacgtca
    23221 agaccatagg attctcctaa ttttatgaca tcttctttta ctggagaacc atctccatca
    23281 atgaatagcc tcattctttt tcgccttctt ccataaatga taaacagtta ctaatccgct
    23341 cagtaaaaga ccaacattga tccattttgc caagataagc agttgtacac tttctatcgg
    23401 cactgattca tggttcagcg tgatcaataa tagatggatt ggctctccca tttcttttgc
    23461 taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc acactccctt tattttgagt
    23521 agtatacttg acaacgatcc ggcgatccat tggaatagct gtatcagttt gataatattc
    23581 cttatttagc cagctgcctg ttactacgtg ccagaaacta agcagaaaga cacctatcgc
    23641 aaaaccattt ggaatcaaga ggaccaataa agaagaactc agtccaagca attgcatagc
    23701 caatggagac catgttaaac gtttcaaaat ccatggtccg actatttttg ctgcaatcaa
    23761 gcctgctgca taaggcaaat acataaaaat cgaaagtttt tctaatccgt acagtatgcc
    23821 aacataaaaa ctgctaaata aaaaaaggaa attccctagc attccattta gaaaagtaaa
    23881 cagatttagc cagcttggca acttccattc cttcttctgg tgtcctaaaa caaacacgaa
    23941 caagatgaac agcagcaaga aaccataaat agcaagatca aaaagccttt catcgaataa
    24001 caatcgggca tttctcaaaa gaaacaataa gccaaagaat atcaaaaata aaacaagctc
    24061 tcgcgtagct accagatttc ttttgatgtc tttgaaatcc agttcgtaac gcggataatg
    24121 actaaccgta tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat
    24181 ctgagtcggt gcaggcaact caatcgcttg atacaggaga aatagccaga tagctgcaaa
    24241 taagtacttt ttcccatcca tattgataaa accttgcgtc ttttcgtgaa aattgaccgt
    24301 ggtattagca ggtacaagcc atgctgcact cagccctagc aaaacacttg aaaaataaaa
    24361 taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa ccagcaccac caatcagcag
    24421 agcgatcatc agcaacgtgt aactatctag accaaatcgt agactgttga ctaaaaatag
    24481 tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga
    24541 gtctgttagt cccaaaaata aaagataagg aataaaaata accgtgttac ttaagataaa
    24601 gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc
    24661 tccttaaaca gtcttataac taatctgctc ttcgatttta gcattaaagg ggaaataaac
    24721 caattttaca
//
LOCUS       DACDKJ010000038        23953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDKJ010000038 DACDKJ010000000
VERSION     DACDKJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.038"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..329)
                     /locus_tag="IV328_001713"
     CDS             complement(<1..329)
                     /locus_tag="IV328_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937850.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVN"
     gene            complement(401..1333)
                     /locus_tag="IV328_001714"
     CDS             complement(401..1333)
                     /locus_tag="IV328_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP8937851.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(1772..2224)
                     /locus_tag="IV328_001715"
     CDS             complement(1772..2224)
                     /locus_tag="IV328_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8937852.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(2237..2647)
                     /locus_tag="IV328_001716"
     CDS             complement(2237..2647)
                     /locus_tag="IV328_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8937853.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            2922..3134
                     /locus_tag="IV328_001717"
     CDS             2922..3134
                     /locus_tag="IV328_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8937854.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            3137..3349
                     /locus_tag="IV328_001718"
     CDS             3137..3349
                     /locus_tag="IV328_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937855.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(3641..3865)
                     /locus_tag="IV328_001719"
     CDS             complement(3641..3865)
                     /locus_tag="IV328_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8937856.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3943..4131
                     /locus_tag="IV328_001720"
     CDS             3943..4131
                     /locus_tag="IV328_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937857.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            4457..4792
                     /locus_tag="IV328_001721"
     CDS             4457..4792
                     /locus_tag="IV328_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937858.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5025..5966
                     /locus_tag="IV328_001722"
     CDS             5025..5966
                     /locus_tag="IV328_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8937859.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            5968..6858
                     /locus_tag="IV328_001723"
     CDS             5968..6858
                     /locus_tag="IV328_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP8937860.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6901..7701
                     /locus_tag="IV328_001724"
     CDS             6901..7701
                     /locus_tag="IV328_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8937861.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7716..8567
                     /locus_tag="IV328_001725"
     CDS             7716..8567
                     /locus_tag="IV328_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8937862.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8564..8767
                     /locus_tag="IV328_001726"
     CDS             8564..8767
                     /locus_tag="IV328_001726"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937863.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIIY
                     RDTERFLTVLGNVYENPELLEVE"
     gene            8768..9229
                     /locus_tag="IV328_001727"
     CDS             8768..9229
                     /locus_tag="IV328_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8937864.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            9255..9707
                     /locus_tag="IV328_001728"
     CDS             9255..9707
                     /locus_tag="IV328_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937865.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            9813..10007
                     /locus_tag="IV328_001729"
     CDS             9813..10007
                     /locus_tag="IV328_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937866.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            10038..10331
                     /locus_tag="IV328_001730"
     CDS             10038..10331
                     /locus_tag="IV328_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937867.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            10361..10801
                     /locus_tag="IV328_001731"
     CDS             10361..10801
                     /locus_tag="IV328_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8937868.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            10876..11343
                     /locus_tag="IV328_001732"
     CDS             10876..11343
                     /locus_tag="IV328_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP8937869.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            12090..12320
                     /locus_tag="IV328_001733"
     CDS             12090..12320
                     /locus_tag="IV328_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937870.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            12479..13312
                     /locus_tag="IV328_001734"
     CDS             12479..13312
                     /locus_tag="IV328_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP8937871.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            13305..14720
                     /locus_tag="IV328_001735"
     CDS             13305..14720
                     /locus_tag="IV328_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8937872.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14732..16261
                     /locus_tag="IV328_001736"
     CDS             14732..16261
                     /locus_tag="IV328_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8937873.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            16353..17231
                     /locus_tag="IV328_001737"
     CDS             16353..17231
                     /locus_tag="IV328_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP8937874.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            17233..17550
                     /locus_tag="IV328_001738"
     CDS             17233..17550
                     /locus_tag="IV328_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937875.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17599..18300
                     /locus_tag="IV328_001739"
     CDS             17599..18300
                     /locus_tag="IV328_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP8937876.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            18315..19205
                     /locus_tag="IV328_001740"
     CDS             18315..19205
                     /locus_tag="IV328_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP8937877.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            19228..19443
                     /locus_tag="IV328_001741"
     CDS             19228..19443
                     /locus_tag="IV328_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937878.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19455..19796
                     /locus_tag="IV328_001742"
     CDS             19455..19796
                     /locus_tag="IV328_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937879.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19796..20167
                     /locus_tag="IV328_001743"
     CDS             19796..20167
                     /locus_tag="IV328_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937880.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            20160..20555
                     /locus_tag="IV328_001744"
     CDS             20160..20555
                     /locus_tag="IV328_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8937881.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20557..20934
                     /locus_tag="IV328_001745"
     CDS             20557..20934
                     /locus_tag="IV328_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937882.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20935..21543
                     /locus_tag="IV328_001746"
     CDS             20935..21543
                     /locus_tag="IV328_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8937883.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21543..21884
                     /locus_tag="IV328_001747"
     CDS             21543..21884
                     /locus_tag="IV328_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937884.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            22126..>23953
                     /locus_tag="IV328_001748"
     CDS             22126..>23953
                     /locus_tag="IV328_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP8937885.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNI"
BASE COUNT         8487 a         3534 c         4772 g         7160 t
ORIGIN      
        1 ctattaacgt tttttacaag tacatcatct tttatatatt ttgaaattaa tgatagataa
       61 acctgagttg ttcgatagga actttctttt acagttggct tgtatttttc atcccattta
      121 gtttttaatt ctccaaaagt tagtggtaat tcttcatttc gttttatttt ttctgcgatt
      181 ttttcattca aaagtaagtt agcttgattc caagcttggc gtgaattgct agataaagta
      241 acggacactt ttctcttttt ttcagttaag ggatccgtat aacgttcggt gtacttaaat
      301 tttttgtcat ttattttttc taaccacatt atttctattc ctttcgaata tacgttccta
      361 ttaattgtta aaagaaaaag cccgaaggct ttttatatat ttaatctttt gacaattcta
      421 aagatcgtct atattgttca gctttagcta attttgtaaa tgttactgtt ccattataat
      481 tttctaaaac aactttctct atttcgtcta gtgaaacttt gaaaaattct tttctttgat
      541 taattttatt aacttcttgt tttctaaaat gattatgcaa tttagtttcc aaagacggag
      601 cgtcctctga aaaaatcatc gcatgaacgt caaattcaaa tggaaccgat gcactactca
      661 attcttttat tctatccata ggttctaatc ttctagtcat accaatttta tatatgtctt
      721 caccaaaaga cccaatatta ctaattacat acacataacc ggctctagta tttaattctc
      781 tttgtaatac attttcacgt ataacttcta attcttttaa tttttcttcc agttcttgaa
      841 ttttatttgc atataattct ttttccacat ctgcattagc tttttgcata tatgcgatta
      901 atttggaaat ttcagaattg aattgacgtt gttctttatc aatacgtttc ttttcacgat
      961 caatttctct acgaactttt tcctcttcta tcatctgttc ttttattgct ttttgttgtt
     1021 ctttttcttc ggtaattttc actaaataag agtaatgaag ttctagttct tgaagtttaa
     1081 actctaaaag acccttcctt aattcagcat tgtcattctt gaataaatta ttaattttat
     1141 caaatatttt tatgattttt tccctagtcg agtctatatt agaaatagtt agtttattga
     1201 gtaatccttt cacttctgaa tcaaatggaa tgagcatttt tttctttaaa ttatttttct
     1261 ctcgtttatt taaaataaga gggacagaaa cagcatcgtc aataatatat tccttttctt
     1321 ttaattttaa caaagaaagc ttatttttta tttcagaaga tgaagtttct tctatatttg
     1381 aaacgataag tgaagaatag acatttttta atgtttcatc atacttttga tttatttcat
     1441 catttaaatc ttctttctct ttttttaata aaataatatc attttttaac atagtaattt
     1501 gtttatttaa atctgatagt gtttcttcct tacttaatat aaatctttct ctatcatgta
     1561 atttatttag tttttcatca tttttttgtt ctaattcata agcttctttt tttattgtgt
     1621 tagctttcga cagtttagtg attcctataa ttaataaaat aagtccaatc aataataaca
     1681 ttcaaaactc ctcatttctt tgatataatt attgtagagt ttactcagaa atgaggaaag
     1741 agtccatgtg aaagcatggg cttttttatt tttaatgttg taatactaaa tgttcaagat
     1801 tatgaggaag ccctaaatat tcaataattt ggaactgact catatcagat aaatcttgtt
     1861 catctattgt tctagttaac atttcaattg caaacatatt agcttcagct tctataccat
     1921 aaatcataca actaaggttg tttgctctaa aagctgctgt gcaaatacct ttatggagtc
     1981 taacgtgggc tatttcatgc cataatacaa atcttttcat agaaggtgat aggttttgat
     2041 taattaatat cacataacat cttttattgt gtatcgtttg tcctaatgtt ttattcccta
     2101 aatcagtttc aattaaatct atatcagaat atttgattat tttatatgga ttaatcgcat
     2161 cagatccaaa ctctacaaat aatttttcta tttcttctaa tatccagtat ttgtttaaat
     2221 gcataccaaa gcccacctat tgacgatatt ttttaggagt gaattttttc tttgctttaa
     2281 ctttggttaa ggcaagtgca agttgaatgg catcgtataa tttttcctta tcttcatcgt
     2341 ctaaaggttc accataataa tttgtttcag catctgaatt caagccttca agtaattttt
     2401 cagcttgtaa agcaatatct tgtttatctt tttctgttaa atcataataa tggcgtttat
     2461 cagtacgtcc taaaagataa tcagttgata cattaaagta atctgcaact ttttgtattt
     2521 tatctccaga tggagtactt ttattccatc tagacatgct tccatttccg aaatttaact
     2581 ttctttcaag ttctgccaac gtcattgaac gttcttttaa taagattttt atacggtcta
     2641 atagcatagg atatgcattc ctttcaaaat accgacaaaa aaagtatttt tctgcaaatt
     2701 atctatttac aaatagagaa tttgcagtta tactagtttt gtaagcaaga tatttacggc
     2761 atacaaagac taaaagcaat aaactaatag tctccccaga catcagtaag ctcgcttttt
     2821 gtgcgctgta ttttgtatgc ttacataata gcatatttgc agtttttgtc aaacttatta
     2881 gacaaaaata ttagaaaata ttcgataagg gggtatgaag catgacttta aaacagcaag
     2941 tattaattgc tttagtaaag aaaggttgga gtcaaagaga gttagctcga cgaatgggta
     3001 tatcaattac ttatttacga gatatcttca tcgaaaaaag aaaacctaag gaacgtttaa
     3061 aacaaatcga agaacttttg gatatcaagt tagaagtaga ttctaatgat cagccatcag
     3121 aacaggaggc gtaaaaatgg tacaacgttt gagcaaagcg gagtttaaag agaaatataa
     3181 gtattctgaa agtacttatc aaagaagaat gaaggaattt aaaaaatcac gttttagtga
     3241 ggggtataac gcagttactt ccaaagaaat aacaattgat gtcgatttat atgaacagtt
     3301 tagaacatgg cgttctaaaa atcgatttag agttagagct gtttgttaat agctaaaaaa
     3361 tctaaattca ccacttctga aaagaggagt tgctgctctc ctttcaagtt tgccaaagtc
     3421 cacgtttttt cattttggca actcctcttc ttggaggtgg tgacggcgag agacgaagga
     3481 gggatgacat gaaggaagaa tcaaaaatag ggattttgaa tgagaacaat tacacaagat
     3541 taattattga aacagaagaa gggaaaaaag tagcagaagt tacattgacc gatgcaactc
     3601 ctgctgacgg ttatcgtatt cgattaactc ctaaatatga ttaacctttt ggtgggtgtg
     3661 gatcgtttcc gtgactatca cggctttgaa tttttccatc tctgccatga ataaatagct
     3721 cagatccttg atttttagaa atctctctag cgatttttac tgcttctgat tttgtattag
     3781 tatgtgcagt cgctttagaa tttccagcac ctttgacatt ccaaccacca ttggaattag
     3841 gtacaacatg ttgatttttt cccatagttt tcacctccat ttctatagaa atattttatc
     3901 aaatagaaat ggagaaattc aataaacgaa aggagaagtg atatggcata taccttgcaa
     3961 caagaacatc aaattctcgg tttgattaaa caacgcagga aacaattaca agatgaccgt
     4021 gcagcgctta gaaaagccga tgagctatca gataggcaag ctgaactaat tgcttctgaa
     4081 cttgaggatt tgagaatgct agaaataaaa aatagggaga ttagattatg aagaagacag
     4141 acacactttt tataggattc attttggggt tattagtgat tgtagcgcac caaagtatta
     4201 tcgggggaag cttgttcgca gcattgatgg ttttaatcaa tctgcttgat tcaaaagaaa
     4261 ggagcaacta tggcacgaga agaagcgcta aaaatcggta aagtgattgc tgataatcgg
     4321 tggggaaata gccgccctat tattttaagc aagcaacata tcgaaaagca aaaagcatgg
     4381 caacaaataa aaaagtgact ctgccagcca gcaaagagtc acatacaaat ttataaaact
     4441 aggagaatta tatcacatga ataagaaaat tgaaaatctt atcgaagaat tgaaacgtga
     4501 atgccaaaag caaggtgttt ctattatttg tacagcccaa aaagaagggg aacttaaaag
     4561 tctcgtttat ggtgagacaa cagaaatttt actttgtctt gctatgcaag aagaacattt
     4621 agatgaaaat ctcccattgt cagcacatat tatgcgtaga attgctgtgg atgcttacga
     4681 acaagcgaaa aatgaagaag aaaatcaacc ttcaaatcat acttttgtta ttaataacaa
     4741 ggaagattta gctgatgtga tgactcgtat tctcaagggg gaatttcaat gatgaatgaa
     4801 aaaacaattg atctactcat tattttattt ttcacgttag tgattgtagc gaaaagttat
     4861 gtaatctttg gcttgttatt cgcaattacc atgcttgtgc tttgcgtgtt agcaagcaga
     4921 aaagaggtta aacatgaacg agttagcaaa tcttgacaac tatttaactg atcctgatta
     4981 tgctaaacca ccatatgaag caccaattga tgaggaggat gaagatgagt aaatctacct
     5041 tagaaatgag ccatcaagaa tggcttgaag accgtaagaa aggcatcgga ggttcggatg
     5101 ttggaacaat tttaggattg aataaatgga aatctcctta tcaactatgg ctagaaaaaa
     5161 caggacaagt agtacttgaa gaatcaggaa gtgagcccgc ttattggggt aatgttttag
     5221 aagaagtggt tgctaaagag tttcaggaac gtacaggcaa aaaagtacgc agaagaaacc
     5281 aagtatttga acatccatta catccatttt taagagcaaa tattgatcgg gaagtagtgg
     5341 gagaaaatgc cattctggaa tgcaaaacag ccaatcaatt tctcggtaaa gaatgggaag
     5401 gcgaagaggt accgcttagc tatctctgtc aagttcaaca ttacatgaac gttctaaaca
     5461 aagactattg ttacatcgct gtcttaatcg gtggtcaaaa atttatctgg aagcggattg
     5521 aacgagatca agagctgatc gatacaatca ctgaacaatt agtagagttt tgggaaacaa
     5581 acgttcttgg aggtatcgag ccaattattg atggtagtca agcgacagct gatttcttga
     5641 aagagaagta tgcagatgtt gaagacgttc aaacgacgtt accaattcat ttcgatgaac
     5701 tagttgaaca gaaaaacgaa ctcaaacgga ctaagaaaga aattgaatca gctatccgtc
     5761 aagtagacaa cgagattatc agcgagctag gtaaacgtga agccagtatc ggtatcactc
     5821 aaaaaaacat catcagttgg aaatttgtcc gtacgagacg tatgaactcg aagaaactag
     5881 cagagaaata tccagatgtc gcaaatgatg aagagattta taacgttact gaatcaagaa
     5941 ggctaaccga aagggagatc aaataatatg gcaacaaatg aatcgttaaa aaatcaattg
     6001 gcagcaaagc cacagaaaca agttgcacca ggtcaattag gacttaaagc tttgatgaat
     6061 acaccaacaa tgagaaagaa gttcgaagag gtgcttcatg acaatgctaa tgcttttatg
     6121 tcgaatgtta tgactcttgt atctaatgac agttatcttg cagatagtga accgatgtct
     6181 atcatgagtg gtgcgttaac tgctgcaaca ttaaatcttg ggctagataa gaatttaggt
     6241 tatgcatatt tagttccatt caatagtaaa aacaagcaaa caggaaaatg ggaaaagaaa
     6301 gctcaattca tgcttggcta taaaggatat atccaattag cccaacgatc aggtaaatac
     6361 aaagcattaa atgtgatcga agtttacgaa ggagaactaa aaagctggaa ccgactgaca
     6421 gaagagtttg agtttgatcc aaatggtaga acgtctgatg aagtcattgg atatgttggc
     6481 tatttcgagt tactgaatgg attcaaaaaa actgtctatt ggaccaaaca agaaatcgaa
     6541 gctcatcgga ttgctaacaa taaagatcgc gataagacaa agttaagtgg tgtgtgggca
     6601 tctgattaca atgcaatggc acgaaaaact gttttgagaa atcttctttc taaatgggga
     6661 atcttgtcca tcgaaatgca agaagccacc acatcggatg agagagtcca aagggttcaa
     6721 gaagacggca gcattattgc tgaaacagaa gttgaagaag atattcctga aagaaaagaa
     6781 gcagaggtta tatctgaaga aaatgaagat gtacaaactg gattatttga tgcatctaat
     6841 ccgccgttaa acaaataatg agggagcttt ctcccttgct tttctagagg agaaatacga
     6901 atgaacacag gatatgtcaa attacatcga aaagtgatga attcattcgt ttggaccaat
     6961 ccttatatgt ataaattatg gaacttgtgt ttgatgaaag cgagtcatga gaatcgcaag
     7021 atacttttta atggaaaaga gatacaagtg aacagcggag aattcgtcac agggcgcgat
     7081 gctatcacat ctgagatgaa cgaaggcgtg aaacgtgaac atcaagtgaa cagcggttct
     7141 gtatggagat ggctaaaaca atttgaaaaa aacggaatgt tgaacatcaa atcaactacg
     7201 aaatacagcg ttatatcaat aaaaaactgg tctttgtacc aaagcagtga acaacaaatg
     7261 aacatcaaac gcacaacaag tgaacaacaa gtgcacacaa acaagaatga aaagaattat
     7321 aagaatgaaa agaataataa aacatcatcg tcgcaaccac gcaaaaagcg tgtttacgac
     7381 accgactcag tttactacat tctcgcggag gagttattca aacagatttg tcagaatcag
     7441 gaaatcaaaa agccgaatct gcaaagttgg gcagatgaca ttcggaaaat gatcgagatc
     7501 gacaagcgga ccgaaaacca ggtacgggga atgattgaat ggagtcagca caatgtgttt
     7561 tgggcatcga atattttatc tgctaaaaaa ttgcgagaaa aatacgacac aatggcagcg
     7621 caagcaaatc gtgattataa aacaaaacaa actaaaacgc ttgaatacga gaaatttagc
     7681 acagatgagt tgcctatttg agaggaggcg taggcatgga aactgttggg gaaatcatgg
     7741 aaaagctgat acagaaagtg cttgtccaac gtggcgaatg tcctgaatgc ggacagcctt
     7801 tgtatggatg gcgtacgaag aaccctgatg gctcagaacg ttgtaagcca acctgtatgc
     7861 aatgtgggta taaagcattg cgagttcagg aagacttaca aacagaacga atttacaacg
     7921 agagcctgaa agcaagagca atcaattttt tcaaaggtgg ttctgttgtg cctaatcaag
     7981 cgttgtttga ttgcacattg cagaattatc aaattgtcga tcaagaaaca agacaagcgg
     8041 ttgaagtaac caaacgcttt gttaattcag tcttgttagg aaatccaagt caccttgttt
     8101 taactggaaa acaaggaaca ggtaaaagtc acctagcgat ggcagcggct tgggaagtct
     8161 tgaagcgatc aaactacgat aaaaaaatct tatttatcgg gttacaggaa atgctggatc
     8221 aaatcaaatt ttcgtacaac aatcctgaac tcagaaaaac gattgaggga tcgttgattg
     8281 cagatatcaa aacagcggat ttagtcatca ttgatgatat cggttcagaa ctaggaaaag
     8341 atgcatcaga tagtcgagcg tttggcataa acacgctaaa ttcgttcttg gatgcacgac
     8401 agaacctagc aacgattatc acaacgaact tgcttggtga agaactgaaa aaagcttacg
     8461 gtacgagaac aatatcaaga atgtttgtca actctgatgg atttacgatg gtattttctc
     8521 aaacagcaga caagcgcata aaaccagtca aaggagcgat cgcatgaatt tgactaaaca
     8581 agaaaaaaga atctgtcgtc agataatgtt gcaaattata ggtgatcgtg acatggacga
     8641 attatctcaa caagagagtc gaatggttcg gaagataagt aagctaatta tttatcggga
     8701 tacagaaaga tttctcactg ttttaggtaa cgtctacgag aatccagagt tattggaggt
     8761 agagtaattg ccaaaaatat tagatgcatg ttgcggtagt agattgttct ggtttgataa
     8821 gaacaattct gacgtaacat ttatggattg tcgtcagcag tatgaagaat tatcaacagg
     8881 tcatgtgatc aatgttgatc cagatgtggt cgcagacttt cgcgatatgc cttttgaaga
     8941 tgatgtgttc gatatggttg tatttgaccc gccacactta agacatgtcg gcgaaaactc
     9001 atggcttgcc aaaaaatatg gaaaattaga cgaactttgg ccagaagaca tcaggcaagg
     9061 ttttgcagag tgtatgcgag ttttacgacc ttcaggaaca ttaattttta agtggaacga
     9121 ggaacagata ccactttctg atgtgttaga ggctattgga gaacagccat tgtttggtaa
     9181 taaacgaagc aaaacacatt ggcttgtgtt tatgaaaaaa ttcagctaac ggaggaaaca
     9241 aaggaggaaa agtaatgaag attgtattaa ataaatgttt tggtggcttt gggttgagcc
     9301 atgaagcaaa aatggaaatt tttaaaagaa aaaatattga agtattccca tacatcaata
     9361 actttagcta tgactcagat gatgaataca cgagatacac aggccaaaag ctaggttcga
     9421 tggattttat ctactatttc aaaaaagacc cgaaaatcga caaagttaca ggaatttata
     9481 gcgaaattga ccgactgtac ggaattgctg atgattccag tttttcaagt gattcaaaca
     9541 gaggtgataa ggacctcgtt gctgttgtcg aaaaactagg aagtgaggcc agtggaccat
     9601 atgcaagtct taaagtcgtt gatatacccg atggggctga gtgggaaata agtgattatg
     9661 acggcgttga aactgcacat tatggttttc aaacaggtag tatttagtcc actaatgaca
     9721 actaaagagg aggcagaagg atgagtgaag atgaatactt tgaacagtag tctattaacg
     9781 acagagattt ttaactggaa aggggtgtag tcatggacgc atcggaagta gtaaataaaa
     9841 ttaatagtct caaagaggtt ttcggagacg tcgaagtagt gataaacgca caaggctatg
     9901 acaacgaatt ttttaagcag attaccgaca ttagcattca gcaaggaatg gaagacgaag
     9961 acggaaactt tatcgatgaa gtagccattt tagtgacttg cgagtaagta attgacagct
    10021 aaagaggagg aagcggaatg aaactaaaag acggatttta ctccagcggc cacggcatcg
    10081 gcggtttaat gctagatatg ccgacaaaga atcctaaaac acgtaagaaa ccaaaattca
    10141 aagtcggtga catggttcgc tgcgaagcag aaggattcat ctatccattt cgtggatatg
    10201 tagaacacgt ctataatcac tcagcaatca ttcgcattga aaacacgatg gaatgtgata
    10261 agtggttagc gaaaagcaaa gagaatttag cagtggtgag attggtggac atggaagtta
    10321 taaaatctta attttgcttt tttacaacta tcctacaaca atgagcgtta ttccacaact
    10381 ggaaggagaa gaggcaattg tgaacatttt agagaatata gatattaagc aaacaaggaa
    10441 aaatgctaga cgattactaa aaagatacag aaatttagaa cgtttggtcg gaccagtgaa
    10501 aatagacttt tccgtgatga ctgttaccaa aaatttgaaa ttcacaattg acagtcaaaa
    10561 cgaagaaatc attgaagcga taagtactcg agattcggtt atcgaagcat taacacggct
    10621 aagtagaatc catttccagg tactttatta tagttattgt tttcctaata agatgtcaat
    10681 gtatcaaata ggagaaaaat taggatattc cgatagaact atcgagagaa tgaaggcagt
    10741 agctttggtt gaatttgctg aggcgtataa atcaggagaa ctcatttcac gtacaaaata
    10801 aaaaaagtcg gattcctccg actatgaata atatttccga cataagtatt atatcataat
    10861 tgggggaatc agaggatggt acttttcgat gtaaagaaat atgagacacc aagcgcgaaa
    10921 gatgttgata tggaacgcac aaaacataat gtcgctgtgt ttctttcagc atatctatca
    10981 gctagatgta gagtagggca acatcgagaa ccaaaagtga cagcatctta ttccttggtt
    11041 ccaccttcta cagctgatca tatatttgaa gcagaaagaa tgctgatcga taaagaagaa
    11101 gcacaggaag aatttgagta tctgcataaa ttgtttgttc gaggctattc agcgatacag
    11161 catccgcata agcctgatgt gactgaaagg cgcaagaaga tattctatga tcgttatatc
    11221 aatggtctgc ccatttatgt aactgctcaa aggaataata ctagcgaaga atcggttaaa
    11281 gtagaatcaa acagaattat catccaattt gcttcatcgt tagaactggt tgctttcaag
    11341 tagccagttt ttacactttt tatacccttt tattaccaat ttggtttcca ttttatacct
    11401 tttttatacc aatcacttac ctattcaacg ttgtattatg atagtgtcga aaaattagga
    11461 aacaggatcg acaaaataaa atgtaaggga ggaaatctcc ctcatcgttt aattaagctt
    11521 cgataggcag caacggaaat attaagaata aggatgtgaa tttcaactcc ttctaaattg
    11581 ttcttattat ctatcatccg ttgctgtcta ttgttttttt aattattcac acgataacta
    11641 aaggtaagtg aagagaaatg attccattaa taatttcaat ttttgcgctt tgtcttaatg
    11701 tatatatgat tggatttaaa aatgggcaaa ataaaaaata gtagcagcca agaataattt
    11761 tatagtgtca ctgtggcgga aagggtagac gctaagcatg tgtgctaggt caatgcttcg
    11821 gcaaccatgc aaggttcgat tccttgccag tgactttggc aaccgaggca tcggcggttt
    11881 aaaaatatag gggtgcgcaa tttcgtacgc gttttgtgca tcgtgtaggt tgctattaca
    11941 taactggtta ggttagattg agatttggga ttcggtacaa atgaatcgtc aaatgtctca
    12001 agcacaggat cggaaacgtc cctgcctgtg cattacatat tagatcactc tttgagtggt
    12061 ctttttcttt acataaagga ggctacataa tgagaaacta ctggtatgta tcgctaacta
    12121 atgaatatcc tcgaaccatt gatgattgtt cagtgcgtgt tgtgcgttct gtacaaatca
    12181 aagggaagta ctctattgtc gaaatgggga gagaagcaga accatgtgag attgatgcgt
    12241 gcaagctcgt ttattgcggt catggtttct atgatgaacc aaacattcaa aataatatta
    12301 acaagaattt gagggattag aatgcaagaa atggcttaaa aatctccaat caggacaaat
    12361 caagttagac aaagtttccg atttaaagat attgattgaa gctgatttaa tgctaaaaga
    12421 tattgaaaat tagaaaacaa aactcaacct aaacagaatg tgaggtggtg tgtattgaat
    12481 ggcaagacaa cgtgatccaa gacgtgatga agccaaaaga atttggttag aatccaacgg
    12541 agaaaagcag ttaaaggaaa ttgcatctga attaaatgtt tcagattctc aggttagaaa
    12601 atggaaatcg caagacaaat ggagcgctga attgattagt aacgttacca atggcaaaag
    12661 taacgttact aatcaaggtg gcgctcctat tggtaatcaa aatgctaaag gtaacaaagg
    12721 aaatagccga gcttctccgc cagtgggtaa taaaaacgct ttgaaaacag gtgagtatga
    12781 aacaatattt tttgacacgt taaccgataa tgagaaggac atctattcta gtttgaatga
    12841 tgatccttct tttgctttgt ccgaagaaat acgacttctt aaaataagac aacttcgaat
    12901 gatgaaaaga atccaacaag ctgaagctgg actaaatgat gaagaagtcg aacgattgca
    12961 gcagctaaga aagattaaaa ctccgattga aaaaaatggt aaaaagctag aaatcaagcg
    13021 tgaggttatg caagatgtgc agattagcag aaaaaaacat cgcaaaattg atgatattct
    13081 ttcaattgaa gattcattga ctcggattag caaccagtta gctaaagcca tcaagcaaat
    13141 gaatgaactt tatatgaatg aatacagaac tgatttaatt aaagctcaga ctgataagat
    13201 ccaagctgag acaaatgaaa ttggcggaaa taattcaggt gaagaaatag aagaatggaa
    13261 acaggcagtt ttaaatgccg caaacaaacg ggcggtgaaa gaaaatgaat aatgaattta
    13321 ttccttttgc tgatattggt tctgccattg attattacta tgataaacca gtagcttttt
    13381 gccaagatat tttgcatttg aatcctgatg aatggcaaga aaatgtttta aatgatttag
    13441 ctgaattttc aaaggtttct gttcgttctg gtcaaggagt tggaaaaaca gcattagaag
    13501 caggagcaat actttggttc ttaacgtgtc gaccctacgc taaagtaata gcaacagctc
    13561 cgacaatgaa gcaactttac gatgtacttt gggcagaggt agctaaatgg ttaaatgata
    13621 gcttgatcaa aaacttactg aagtggacaa agaccaaaat ttatatggtt ggtgattcag
    13681 agcgttggtt tgctacggct agaacagcga ctaaaccaga aagtatgcaa ggttttcacg
    13741 aggaccatat gttgattgtg gtagatgaag cttctggtgt gtctgatcca attatggaag
    13801 ctattcttgg tacgctatca ggttttgata ataagctgtt gatgtgtgga aaccccaata
    13861 atattgaagg tgtcttttat gattcccata attcagaccg tgataaatac agagttcata
    13921 aagtatcaag ctatgatagt aaacgtacaa acaaagacaa tatagaaatg attcttaaaa
    13981 aatatggaaa agaaagtgat gttgctcgtg tccgtatttt tggagaattt ccgaaaggtg
    14041 cgttggattc atttatcagt cttgaaacag ttgaattggc tacagaaaaa caaattagtg
    14101 attctttagt caataaaaca aaggttgctc atattggtgt cgacgtagct cgatatggtg
    14161 atgattctac gattctcttt cctagaattg ctaccagggc attggagtat gagaagtatt
    14221 caaaacgtag caccatggaa acaacaggat atgtcatcaa catggccaag aatctaatga
    14281 gtcaatatcc gagtattgat aaagtgatga ttaaagtcga tgacactggt gtcggaggtg
    14341 gtgtaaccga ccgcctagaa gaacttatag aagacaaaca ttatcctttt gaggtgtttg
    14401 gagtgaataa cggttcaaca tcagaagacg atttttacga taatttaggt actcaactat
    14461 ggggaaacat caaggaaatg ttagaagaaa atatgacagc aaatcttaac ggagaacagc
    14521 ctgttattga attgccttct gatagttcgt taatcaaaga attaagtact cgcaaattca
    14581 aaatgacaag tagaagtcgt atacgtttag aaagtaaaga tgatatgaaa aagcgaaata
    14641 ttggtagtcc cgatattgct gacgcactgg ctttagcgtt ttatgagcca ccaagtcact
    14701 atcaatttat tcaattttag gaggtgagct tttgattagt acagtattat ctttagagag
    14761 atataagaag ctacgtgtta aatatgcaac acaaattgaa gatggaatgt ttgatccgaa
    14821 tggttttata gaagatatga aaccattttt tgctgatcgc gaaagaaaat atctagctta
    14881 tactagcgaa aagaatgaaa tagataatag accaaagcct aacactgata ttgtaaaagt
    14941 taataataaa cttcatgctg gtatgtattc aattgtcgta gaccaagcag tcaatcattt
    15001 tactggtata cctatcaaat gggattacga tgtatcggaa caaaaaagaa cactcattca
    15061 aagattaaaa gataaattct taaaaaatga tatagaactt ccgacagttc cagaagcttt
    15121 taacaaatta acaagcaatc ttgattccat gagattcgca atgcttgatt ctgaaactgc
    15181 aacttttcaa ggagcttgtg gagtagcttt ccgattgcta gagcctgtgg aagaagatga
    15241 tggttggaag ttaagagcaa gtaatattga accttggaga gcagaaagat atggaaatgc
    15301 cggaatctat atcaaagaaa agtatgactc ttaccagaaa aaattttttc aagaaatgaa
    15361 agttatgaca agaaataaaa ttctgacata tgcttgctat ggtgatttga attttgttac
    15421 aagcggaacg ttcaaaaaaa ttgatgagac cgataaccct ttggggacga ttaccttggc
    15481 agaatttaaa aataatacga atcgttattg tgattttgaa gtagctgagg aaattggaga
    15541 tgcaattgat cgtgctttgt ctgatcaaca aaacgaaatc gaacaattta agcttgcgta
    15601 catgcttgtt actggcacaa caatgagcaa aggcactgct aaagaaatga tgaatcagct
    15661 agggattatc aacttaaaag cccctactgc aaaagctgaa tatgtcacaa aaaatttagc
    15721 aaaagatttc aatgaatatc atatggatct attgaaaaaa cagttttaca ccatttgtaa
    15781 agcaatcgat tttaacgatg aggtgtttaa atcaaacagt tctggagaag ctcgcaagtg
    15841 gcaaatcatt agcctagaag cgaaaacaaa tactaaagag caatatttta gagaaggatt
    15901 gaaagaatgc gcagaaacaa ttgctgcttt tcttaagttt catgacaaag tagaaattga
    15961 gcctgaaaaa attattttta ctttctctag atctttgcca actgatttaa gttatttagc
    16021 tgaagcttta cctaaacttg caccatacgt atccaaacga actattcaga gccaaattcc
    16081 atttgtaaca gatgttgatt atgaaaatga aatgatggaa ttggaaagtg gaagtgctta
    16141 tccagatagt gaatacaatt ttggcggagg tggcaatagt gacgatagaa acgaagtatt
    16201 ggaccaaacg tcgagaactg gaggatcaag caaggctcaa acaagaaaat cagacactta
    16261 aaaaattaac tagtgtattt cctgaagcac tgaaagagat acaagcaaaa ctattatcac
    16321 aagctgactt acacaatatc acttatccag aaatgatgga gttttacagt acaagtaatc
    16381 agaaaaaata tcgtgaatat gtggaaaaaa attataagtc attaaaaatg tatgatgcaa
    16441 aatacaaaga gtttatcgat gaattttttc caccatttga ctatgcaaaa gtcaatcgct
    16501 tattacaaat acgatcagat gtatttaaaa ttcttgcaga atatgcgatg gatgcagatg
    16561 tgaatcaata tttttctgat cgcttagagg aaattcttca aagaacatat tcttctaatg
    16621 ctaatgtttt tgttcaactt ttaaacgttg atataccaaa ttatttacca gaaaatgaaa
    16681 ttaaacatta tttgaactat ccatggtcag gaaagacatt ttcaagaaga ctttggggaa
    16741 atatttcatc gcttgaacag aaactctcta atgctattgt aaaaagtgtt gctagtggag
    16801 aaggcgttat acacgcatta aacaccatga gattagactc agaaatttgt gacatgttta
    16861 agttagaaga atcaaagtat aacaaagcga tagaaaatct cgttcgaacg gagtatgcaa
    16921 aatttgcaca agatggtatt gaaaaatcat atttagaaac aggtattgag gaatacaacg
    16981 tattgactgc aaaagatgag agagtttgcc ggatttgtgg aggaaaggca agtaagaatc
    17041 cctataaact gaaagatgct gtcataggtg aaaatcgagc acctttccat agtcgttgta
    17101 gatgtacgga tgttcctaat ttaccaaaat taggaaagga tattgatgaa gaatatgacc
    17161 gtttatttgg cgatttatta gatgagtttg cacatgattc ttttggaatt aatttgaaac
    17221 ggaggaagta gaatgaaaga tttttttgaa gcagtactaa caattaatgt aaatgctgat
    17281 attgcagaag cctacaaaac agctattgag tctgagaacc atcctaatgg cttgagagac
    17341 cattggaatg gcaattatgc ctacgtggtt attggcgatc aaactgttaa ttatcaagat
    17401 aatactccag ttgataagaa taccgttaat ttaacgattc aattattatc tcattcatta
    17461 ccaaatttaa aagaaacagt tgattggtat gaaaagatgg gatgtattgt tgttagaact
    17521 gactacaaag aaggaaagtc tagtaattag gcttttttat tttgtccgaa atgacgttaa
    17581 actagcgcaa tactgggctt ggttgaatgg tggggcgcaa ctattaaaac tcaaagcaat
    17641 gcggggcgtg aaaacgaatc gtggggcgaa aggagaatga gcatgaaaca caaatcatta
    17701 atgccaatga atttgcaata ctttgccgaa ggtgacgatc aaaagttttc ttttgatgac
    17761 ttcaaatctt ttgtggaatc caatgaagaa gctcaaaaat ttattcagtc acaatctcaa
    17821 agtgttgccg acaaacaatt ggaagcttgg aaacaaaata acttagataa aattaaacag
    17881 gataccatca aggaatatga ggaatctaag aaaaacaagt cacctgagca aattcaactg
    17941 gaaaaactac aagctgaatt tgaagcagaa aaagcgttgc gtgtgacaag tgataataaa
    18001 gcatttgttg cagaacaaat tgctggatta gaactagatg gagagttaaa agagtctatt
    18061 tctcaattta tgctaaataa tcttgttagt tcggatacag atttcactaa gaatgctgtt
    18121 gaaggtttta caagtgtttt gaatgcaatt aatgagaaac atgcagatgc attaaaagaa
    18181 ctacaaatga agtctgcatt tggtggaact caacaatcga ataaccaggt tcagcagaac
    18241 aatgaaacat taacaaatcc agaagaacaa ttaggacaaa ttcttcaaca atttaactag
    18301 gagagtgaaa aattatgaaa aaaacatctt taaataattt agagtatttg gatatttcac
    18361 cagcgattaa tgctatgcaa gtaccaaata cacctttttt aagctattta cttggtgctg
    18421 gaaaaacaga gccagcaaac tcgacagaaa ttaaatggcg tgaatatgat atcaacaacg
    18481 atgattcttc tgaaaaactt gagggcggag aatatccaga tgctgaatca ggtcgaactt
    18541 ggtttaacaa ctatactgaa atttttagaa aatcaacctc tgtatctggt acattagatg
    18601 ctattaatgt gaatggagtc ggaaatgaat taactaatca agtagcacta cgtggtatgg
    18661 aaatgaaaat tgatttgaac cgaaaattga ttactggtgt aaaagctgat gaaaatggtt
    18721 ctaaaggtcg tcgaatgaat gggattttga acttgatcaa ttcagcaaat aaggcagaaa
    18781 cagccactgc gggtgcagta acaagaaaag atatcgatac tttatttaaa ttgatgtatg
    18841 aaaaaggtta tatgggagaa aaactatgct tgatttctcc agatatgcag gagttaatga
    18901 ctgatgagtt agatggaaaa tcaacaaaaa ttgttcagtt cggggaaaga gtaacttttg
    18961 gattgcaaat tggaaatatc gtgtctaatt acggtacagg tattgctcta ctagaaccat
    19021 cattgccaaa aggaacaatt gccgcaatcg atactaatta tgtgaaacta cgtccattac
    19081 gtgaatggag agcagaagaa cttgcaaaaa caacggattc tagacgtatc ggtcttgtag
    19141 gtgaatactc tcttgaatac aatgcttcaa actctggggc aattttaaat ttaaagtctg
    19201 aataaaaggg agttagtact cccttttttg ataggaggaa ttacagtggt aaaaaaagat
    19261 gaaactaaaa aagatgaagt cgtgaaatat agagtaggta aaactaaaaa ttttgttggg
    19321 tttgttcatc ctaaaactcg tagatttatc acagcagatt caaataacga atttatcatt
    19381 tctatagatg ataaagaagc aattgcaatt ttggaagatg caattgatgt taatgaaatt
    19441 taggaagtga tctgatggat gaatcgctaa aaacggaaat cattgagtct acaaaagaag
    19501 attttccaga tttgagtgaa gaacgcataa ctaatttatt agaaataatt ttgctagaaa
    19561 ttgaatcata caacacttgt aaaaatgata tttcatggga aaagttaaaa agcgtgatta
    19621 gtgaagtgtt gtataaaata ataaaaaatg aatcagaaaa aacagtatct tcagttagac
    19681 gtggcgatac aacgattagt tatgcttcaa caacaaatga tgttagtgag ttacttctag
    19741 gctatggtga tttgatacga agagttattg gttgtggagg attggagttt ttttaatgaa
    19801 tgaagcagat attttagaaa ctacttatga agatagttgt attattgaaa gactgacgga
    19861 cattgaagat tctaatacaa atattactat tcaagattac aaaaaagtat atgataatcc
    19921 tatttcttgc gctctttcac aaggtcaaat cgatggacta gcagtcatag aagatggaga
    19981 gatggtaaat gtttcaactg acacatataa attatttgtt catcctaaga ttaaactaaa
    20041 aaaaggagat cgaataacaa taactcaaaa ggccagtggc ttaatttttt ctctatttgc
    20101 tactaagcct ttttactatc ctagtcattg tgaagtgaat ttgataggaa gtgagaaaaa
    20161 tgggtaatct caaatatgaa tcaaatgcag aaaagatcat tgagaatttt aaaaatatga
    20221 ctgtaattgc tcaaaaagaa ggaatatcct tcgttaacga ttcaatgaat aaagtcgtta
    20281 gtttagctaa accattaact cctgtaaaat caggtaattt aaggcgagga tatcgtgtag
    20341 taaaagctag aaaactatca agcggtcgaa ttgttggaga agttataaac aatgaacatt
    20401 attttaaata tgtagaagaa ggtcatagga ctaaaaatgg tggctttgtc aaagggaaat
    20461 tcatgttaac tcgtgcaacg aatcttgcaa atatgtctta tattcctcga agatttaaac
    20521 aaatggcaat aaaaatcgtt aagaaaggaa agtaacatga aagatgaaat cattgctgca
    20581 atcagtagca aattaaaaga aatctatcct gatgggacaa tatatcttga ttcagttatg
    20641 cagtcaacta aagattttta tttcgtttta tcagtaatgg aatctggaac tgaaaatgta
    20701 ggaattgata ttcaaaatgt ttctttctta attgatattg cattgattga taataaccct
    20761 aatagaaatt taattaatga attagtctca cgttgtggga ctttttttaa tacgattaca
    20821 atcgatgagc aaatattatt tccaaaagcc tatttacctg atgaagcaga tggtgttcaa
    20881 catattcgtt ttacattaga atttccacaa tatattgaat ggagtgaaag ataaatggga
    20941 gaaaaaagaa gtaaaaccgg aattatttct gtcgaaaaac ctacttggtt tcctttggaa
    21001 gatgagacag ggaattttcc ggtatatggt actgcgttgc caatgggaac agcagtaagt
    21061 atcaaaccaa cagctaatta tgaaacaaca caagattatg gggattcagt ggttcaagat
    21121 caatttacag cttttggtgg tgcagaagtt gaactggaag caaatggata tactcataaa
    21181 gttttatcta caattactgg agggaaaatt gttaaaggtg gtgccttgcg ttctggagaa
    21241 gatattgcgc aagatggtgc gtttgcatat agacgtaaaa aatcgaatgg gaaataccgt
    21301 tatacggtct tttataaagg acagtttgct ctagattcag atgaaacatc aacgattgaa
    21361 ggaagtaaag taagttttac tcatccaact tggaagggtt catttgtaga tgtaccagga
    21421 cttggatata tgtattcagt tgatgaagat gatgaaggcg tggataaagc aatgatcgga
    21481 aattggttta caaaagtagc aattccaatt gaagaagcag aactttcagg aggtacagaa
    21541 taatgtcaaa atatcaaaca acgattaaat taacgaaaaa agacgaagaa ggtaaatatg
    21601 agcaagtaca atttaaatct gctgaatttt taccaggaac agttgtagaa gatgctgcag
    21661 gagttatgga agaaatgcaa acagcgactg ataagcaatc agttaaaaaa gctttaagtc
    21721 gtgcttattc atttattgca gatactttat ttgagggaca atttactggt gaagattatt
    21781 gcaaaggaat tgatgctcgt gagattgcct cattgacagg aaaattattg aagtctgtta
    21841 ctgcaggttt tgatgaaact tatacagaga cgaaaaaaaa gtaagtgagg cgctcaagtc
    21901 accttcattt aagtattcga ttacttaccg agaattagat ataaagacgc aattactcga
    21961 agcaggttgg acgttaccag aaattgaacg tactgacttg gatgagttaa tgcgtctttt
    22021 tgcttttaga gatgcggtaa aagagcatga agatgttgag tactatgata atttcactca
    22081 attttaggag gtgatacttt gaataacgat gacctaattc tgaagatgat actagatgaa
    22141 tctggtttta ctgccggtat gaataatgct gtcaaaaagt tgaactcttt tgatgaaaca
    22201 atcgaaagaa aaagtagaaa tagtggcagt tcattaggta gtatttggaa aatatttgct
    22261 ggaagttttc tagctagcgg agtaactaga atcgtaggag ctggctttga tctaatcaag
    22321 ggttccataa gtggagcggt tgatcgagta gatacgatga ataacgctct acgaaatttc
    22381 caaaatatgg gatttagtaa ttcagaaatt atgaagaata tcggaaagaa tgggctttta
    22441 tctcaaggta ttcaaggact tcctactgct ttgaatgatg cgataagtca tgttcaactt
    22501 cttgcttctt ctacaggtga tttaactcgt tcaactcaaa tatttaaagc tttaaatgat
    22561 ggaattcttg gtttcggtgg ttcaactgat caagttaatg aagctgttat tcaattatct
    22621 caaagtttct caaacggaaa agtagacgca caaacttgga actcaatgat caacgctcaa
    22681 cttggaccta ctctttctgc tattgctaaa aagatgggaa ttacaatggg agatctgaaa
    22741 gaaggtttat ctcaaggtaa gatttctgtt gaagaattcc aaaatcaatt aatagaaatg
    22801 gataccaaag gtggcggagg acttaaatca ttaagtcaaa tcgctaaaga ttctactaag
    22861 ggaattaaaa cctctataca aaatgcaaaa acagctgtta cacgtggtgt tggtgaggta
    22921 atagaaggat taaataaagc attagtagat tcagatttag gtggatttaa gggaattatt
    22981 gataaagtgg gcagttcaat ggaatcgttc ttaaaagtaa ttgctgcaaa tattcctaca
    23041 gcagtatcat ttttaagtaa tttatttaac gaaattcaaa aatttggttc tgctttgaaa
    23101 ttcatgatgc cctttcttgt tcctgcagct accgcttttg gagcgttcat gtttcaactt
    23161 aaaggtatac cagcaataat aaaaagcttc aataatttta agaatgccat aattggcgtt
    23221 ggaaattcat taaagattat gggggcaata gctgctgcta atccatttgt tttaattatt
    23281 ggtgcagtcg ttggtgcaat agctgttttc ggttacttta tggcaaccaa tgaagagctt
    23341 agaaacaagg ttatatctat ttggaatgat gtgaaagatt ccgtaattgg tgtattaaaa
    23401 aatataaagg actggggaat tgatacttgg aattctgcta aagaaatggc atcaaatgca
    23461 gttgagggtg tcaaagatgc ttggtcagga acaaaggaat ggttttctaa cacctggaaa
    23521 ggtattaagg atggggccac aggtttattt gataaaacag tagagacttc aagaaatgca
    23581 gtcgatagtg taaaaaatgc atggtctaat atgaagaaat ggttttctga tacttggcaa
    23641 agtataaaag attcagccag agaaaaatgg aatggaatca aaggttctat tatgtcagtt
    23701 gctggtccat tgattacagg tataaagaat gcatttttac atgttacttt ttatctagag
    23761 accttatgga ataatttagt tgagattggt aaaaacgtct ttgagatttt aaaaaatgtt
    23821 atacttgcgc cggttttgtt tattacctcc atgatttctg gtggatggga agaaacaaaa
    23881 aataatatga ttgctgtttg gaataatatt aaagaaagtg ccataaatat ttgggaatct
    23941 ataaagaata tat
//
LOCUS       DACDKJ010000039        23227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDKJ010000039 DACDKJ010000000
VERSION     DACDKJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.039"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..212)
                     /locus_tag="IV328_001749"
     CDS             complement(12..212)
                     /locus_tag="IV328_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8937886.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(517..1749)
                     /locus_tag="IV328_001750"
     CDS             complement(517..1749)
                     /locus_tag="IV328_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8937887.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2003..2572
                     /locus_tag="IV328_001751"
     CDS             2003..2572
                     /locus_tag="IV328_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8937888.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2750..3190)
                     /locus_tag="IV328_001752"
     CDS             complement(2750..3190)
                     /locus_tag="IV328_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8937889.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3348..4112
                     /gene="map"
                     /locus_tag="IV328_001753"
     CDS             3348..4112
                     /gene="map"
                     /locus_tag="IV328_001753"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8937890.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4144..5067
                     /locus_tag="IV328_001754"
     CDS             4144..5067
                     /locus_tag="IV328_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8937891.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5143..6282
                     /locus_tag="IV328_001755"
     CDS             5143..6282
                     /locus_tag="IV328_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8937892.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6275..7075
                     /locus_tag="IV328_001756"
     CDS             6275..7075
                     /locus_tag="IV328_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8937893.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7075..7902
                     /locus_tag="IV328_001757"
     CDS             7075..7902
                     /locus_tag="IV328_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8937894.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7880..8614
                     /locus_tag="IV328_001758"
     CDS             7880..8614
                     /locus_tag="IV328_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8937895.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8714..9580
                     /gene="rfbA"
                     /locus_tag="IV328_001759"
     CDS             8714..9580
                     /gene="rfbA"
                     /locus_tag="IV328_001759"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8937896.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9594..10166
                     /gene="rfbC"
                     /locus_tag="IV328_001760"
     CDS             9594..10166
                     /gene="rfbC"
                     /locus_tag="IV328_001760"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8937897.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10188..11216
                     /gene="rfbB"
                     /locus_tag="IV328_001761"
     CDS             10188..11216
                     /gene="rfbB"
                     /locus_tag="IV328_001761"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8937898.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11314..12165
                     /gene="rfbD"
                     /locus_tag="IV328_001762"
     CDS             11314..12165
                     /gene="rfbD"
                     /locus_tag="IV328_001762"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8937899.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12200..14233
                     /locus_tag="IV328_001763"
     CDS             12200..14233
                     /locus_tag="IV328_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937900.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14277..15557
                     /locus_tag="IV328_001764"
     CDS             14277..15557
                     /locus_tag="IV328_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8937901.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15767..16573
                     /locus_tag="IV328_001765"
     CDS             15767..16573
                     /locus_tag="IV328_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8937902.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16585..17805
                     /locus_tag="IV328_001766"
     CDS             16585..17805
                     /locus_tag="IV328_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8937903.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17795..19381
                     /locus_tag="IV328_001767"
     CDS             17795..19381
                     /locus_tag="IV328_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8937904.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19420..21558
                     /locus_tag="IV328_001768"
     CDS             19420..21558
                     /locus_tag="IV328_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8937905.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(21926..22909)
                     /locus_tag="IV328_001769"
     CDS             complement(21926..22909)
                     /locus_tag="IV328_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8937906.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7291 a         3967 c         4780 g         7189 t
ORIGIN      
        1 ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa
       61 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt
      121 cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa
      181 cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt
      241 cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact ctagcggtat
      301 aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt
      361 agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac
      421 ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg
      481 ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa
      541 aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac
      601 catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga
      661 catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga
      721 tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg
      781 gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt
      841 tgctaagaca tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt
      901 aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta
      961 accatctact cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat
     1021 gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa
     1081 tccgataatg atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt
     1141 caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc
     1201 ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc
     1261 ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt
     1321 ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc
     1381 tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga
     1441 aacaacgctg atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga
     1501 ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg
     1561 gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat
     1621 caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc
     1681 tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc
     1741 agataacata ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca
     1801 tttaattatg attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt
     1861 taagtttcat gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta
     1921 attttgcata tcaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa
     1981 aaaattaagg agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt
     2041 ggcagctgct tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat
     2101 cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga
     2161 cttgagacaa gctctttttg agcagatcta tgatcattta caatatgaag tgttcactgt
     2221 tgagcataca gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag
     2281 agagaagaag ttgtatcgtt cactgtactt agaagaatat ggtggaggaa aagagatgca
     2341 gaatttctct tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct
     2401 ttcagaagag cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc
     2461 agctttgatg tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca
     2521 agacagtata gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc
     2581 aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt
     2641 tcttacagcg tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa
     2701 gagccataca gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg
     2761 cgactagttc tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat
     2821 ccactttaac attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat
     2881 catccaccga tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa
     2941 tttttcccaa tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca
     3001 gatcgccatc atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat
     3061 ctgcatctac ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt
     3121 ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg
     3181 ctaaagccat gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa
     3241 catcattgaa aagaaaataa aacctaaaga cacctgggga atgtcatttg gctaaatgta
     3301 tgccgctatg ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa
     3361 aatcagcacg tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata
     3421 aaaatctgcg tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca
     3481 gaaactatat cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat
     3541 atgcgacttg tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt
     3601 tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg
     3661 attcttgttg ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag
     3721 taacgaagaa agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg
     3781 acatcggtca tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt
     3841 ttattggtca tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag
     3901 aagctggaaa agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta
     3961 ataccggaac ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg
     4021 gcggactttc ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt
     4081 tgacatcaca aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg
     4141 tacatgaaag tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg
     4201 actcaaaagc gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat
     4261 ttactcttgt ctttatttcc attgattatt gcttttggta atattttacc gtttctccac
     4321 atcgatcaag agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag
     4381 tttatcggtc ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt
     4441 tctgcattag cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg
     4501 aataaagcgt atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta
     4561 attgtcattg tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga
     4621 cagctgattt tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag
     4681 ttccaaactt tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt
     4741 tatttaattg tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc
     4801 gctactgtcg gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca
     4861 acgaggatca gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat
     4921 tttgctgcta cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg
     4981 acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa
     5041 actaaatttt ctcataaaaa aaagtagaaa caagagttct tattgtaaat ttatatcagt
     5101 aatttataat aattatgact cttaattaaa gtaggtgccc atatgcttgt ttcttttagt
     5161 atggttataa gattttttct gacaatttta ttatcactga tattaacacc tgtattcaaa
     5221 atcatttcag tacaaacagg tatggtcgac aaaccaaatg aaagaagaat aaataaagtt
     5281 ccaatgcctt cagcgggcgg actgcctatc tttatttcat ttgttatatc gactttattc
     5341 ttattccgta aaattatccc tcaatcgtat attgtaccga tcttgattgc ttctttagta
     5401 atcatagtca caggagtgct cgatgataaa tatgagctga cacctaaaca aaaaagtata
     5461 gggatcttga ttgcttctct tattatttat tttgtagcag atatacgtat tgattccttc
     5521 acgcttcctt ttattggata tatccaacta ggctggctta gttttcccgt aacgattttt
     5581 tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg gctggcggca
     5641 gggatttcat tgatcggttt aattacgata ggaatcattg gatatttttt cctccatgct
     5701 tctacagtgt atattccagt tgttattttc tgtcttgtag caagtattat cggttttttt
     5761 ccttacaatt tttatccggc aaagatttat ttaggcgata caggagctct attcctaggg
     5821 tttatgatgg ccgtcctttc actacaaggg ctgaagaacg tgacttttgt ttcatcgatt
     5881 tctttgctga tcgtcatggg agtgccggta acagacacgt tttttgctat cattaggaga
     5941 aaagcaaacc gtgtttcttt ttctacagca gataaaaaac atcttcatca ccgactaatc
     6001 agtttaggct ttactcataa aggtgctgtc ctgacgattt atgcgatggc tttgatgttt
     6061 tctttcacag ctatggtcat gaattatact ggaagaatcg gaacgatcgt attgattatt
     6121 gccatgttgt ttgctgcaat tttattattt gaattgattg gtctgatcaa tgagaaacat
     6181 cagtttattt taaaaacatt gcgattttta gggaataaag agtaccgtac gcaagtaaaa
     6241 cagaaatatt tgaagaaatg gaaccgtcat atagatgaat aacaaattaa accagccgat
     6301 ccttattagt attgtgacgg caaacagtga taaaattttt cggacactag ataattttat
     6361 ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca attcacttcc
     6421 cgagtacaaa aaaagactaa cagagtatag gaaatatccc tacatcgata tctattttca
     6481 tgatgaaaat tccggatttg gttatggaca taatcacaat ctgatagcta gcgattctcg
     6541 ctatggcatt atttgtaacc cagatatttt agtggatgaa gagacagtta cagcattggt
     6601 cttgttattg aaagaacatc cggaatgtgc gatgttagca cctaagattc ttaatgaaga
     6661 tggaacaact cagcacctca tccgtgagca gctggctgtt tttgattacg ttttgcgctt
     6721 tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt ttgaatgccg
     6781 aggattgcct actgatgcta atagttatgt acggatgatc tcaggtagtt ttatggtagc
     6841 tgatattgat aaatttaaag agataaaggg gtttgatgag cgtttcttca tgtactttga
     6901 agacaacgac ttgtgtttga gttttgaaaa agcaggttac aagttattgt acacaccctt
     6961 ttatgcagtt atccattttt acgggaaggg tgcgcatcga aatgcaaaac tgtttcgcgt
     7021 gtttttgact tctatgatga aatttttcaa taagtggggg tggcgttttt tctgatgaaa
     7081 ccagaaatat tagtagtcgt tgtgctttat cagcaactat tcagtcaatc tccagcttat
     7141 ggaccattaa gcaaagcact tagcgaaaag caaattcagc ttattattta cgataatagt
     7201 cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata tcgtttatca tcatgatccg
     7261 caaaatcctg gtttagcggt ggcttataat tttgcgttgt ctcaagcaga tgaaggaaca
     7321 cgttgtctcg tcactctgga tcaggatacg aggctagtcg acgattattt tgaactattg
     7381 cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc cgatgatttt ttctgggagc
     7441 cgacaagttt ctcccctata ttcagatcgt tatatcaatc gtacagctca agcagtagaa
     7501 gttgaaggcc caacagctga acgaatcatg gcaatcaatt caggtgttgc ctggtctgtc
     7561 aaatttctaa aaaagattgg cgggtttaat cctgcttttt ctttagattt tttggatcat
     7621 tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg agatccttcc tgtacgaatg
     7681 gaacatgatc tttcggtttt agactatgaa aaagtttcaa gtaatcgata ccgttctatt
     7741 ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc cagtcaccga
     7801 cgacaattat tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg gcatatctgg
     7861 cggatgactt tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc gttcggtttg
     7921 tatagctacc tttaatggag gcagatattt aagagagcag ttagatagta ttttatctca
     7981 gctttcagca gaagatgaag tagtcatttc tgatgatggg tcagtagatg atacatggga
     8041 tatattaact gaatacgcag aaaaagataa gcgaatccag cttttttcgg ggcctcgtca
     8101 aggattgatc gctaattttg gttatgcgat ccagcagacg aaaggagatc taatcttttt
     8161 ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa aagatcactg actattttga
     8221 gcagaatccg gatcagctag tagtagtttc tgatttaatc attgtggaca gcgagctgca
     8281 agagatccat gattcgtatt tttcgtttcg aaattccaaa gaaggatggc ttaataatgt
     8341 gataagaagt cactatattg gtgctggtat ggctttccgg tcttctttaa aacccgatat
     8401 tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg attggtttgc tgggaggaaa
     8461 gaagactggg ttcctaagag aaccgttaac tctgtaccga agacatgatt taaacgcgag
     8521 tgagattgaa acacattcta aattacggca aaaaatttct tggcgtgttc atctgattac
     8581 ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat ccttaatatt
     8641 tccatgacaa atataacaat tgatgagata ataacaaagt gaaatttgaa atcaaaaagg
     8701 agagcagata tccatgaagg gaatcattct tgctgggggc agcggaacac gtctgtaccc
     8761 tttaacaaag gcaacatcga aacaattgat gccgatttat gacaaaccaa tgatttatta
     8821 tccaatgtct actctgatgt tggcgggaat caaagaaatt ctgattattt caacgccaca
     8881 agatacacca cgtttcaagg aattatttgg aaacggaaat gatttaggac tgcagatcga
     8941 atatgcagta caagaaagtc cagatggatt ggcacaagcg tttatcattg gagaagaatt
     9001 tattggagat gatagtgtct gcttggtatt aggggacaat atttactatg gtggcggtct
     9061 gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc gcgacagtat ttggctatca
     9121 tgtcaatgat ccagaacgct ttggtgtagt agaatttgat gagaatatgc aagctttgtc
     9181 gattgaagaa aagcctgctc agccaaaatc aaattacgct gtaactggcc tttattttta
     9241 tgacaatgag gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg gagaactaga
     9301 gattacggat gtcaataaag catatttgga taaaggaaaa ctatccgtag aacttatggg
     9361 acgcggattt gcttggcttg atacaggaac acatgaatca ttgcttgaag catctacctt
     9421 tatcgaaaca attgaaaaac gtcagaactt aaaagtggct tgtttggaag aaattgctta
     9481 ccgtatgggc tacatcgata aggaacaatt gctggccctt gcacagccat tgaaaaaaaa
     9541 tcaatacggt caatatttat tgaatttggc cgctgaataa ggagtcctta cagatgaaag
     9601 taacagatac aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt ggcgatcatc
     9661 gcggtttctt cacagaaagt tattcaaaag caaaatttgc agaacatgga ttgaaatatg
     9721 actttgttca agacaaccat tcactatcag ctgaagctgg tgtccttcgg gggcttcatt
     9781 tccaaaaagg agaagctgca cagacaaaat tgattcgggt agtcactgga gcagttttag
     9841 atgtagtagt ggatatccgc aaaggtagcc cgacttatgg tcaatgggac ggatttatct
     9901 tatctgaaca taatcatcga cagttgcttg tacctaaagg atatgcccat ggattcgtta
     9961 ctttgacacc aaatgtgaac ttcctttata agtgtgataa ttactataat gcagaagcag
    10021 atgggggcat cgcatttgat gatccagatt tagcgattga ttggccaatt gatccatcaa
    10081 gagcgattat gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt gaagcagaaa
    10141 atccatttgt ttacggagaa atctaagaat taaggagaaa ttttcttatg aaaaacatca
    10201 ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt tcattacgtg gtaaacaatc
    10261 atccagaagt gcatgtcact gttttggata aactgactta tgcaggaaac aaagagaact
    10321 tggccggact tcctagcgat cgcgtagaat tggtcgtagg agatgttgca gatgcagaat
    10381 tagtggaccg tctagtaaaa gaagcggacg cagttgttca ttacgcggca gaatcccata
    10441 acgataattc attaaaagat cctttcccat ttgtacaaac gaatatcatt ggtacgtata
    10501 cacttttaga agcttgccgc aagtatgatg tacgctatca tcacgtatct actgacgagg
    10561 tttacggcga tcttccatta cgtgaagacc tgccaggaca tggggaaggc gaaggagaaa
    10621 aattcactgc ggaaacacca tataatcctt caagtccata ttcttctaca aaagctggtt
    10681 cagatctgtt agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc atttcaaact
    10741 gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt tattcctcgc caagtaacga
    10801 gtatcttaag cggtattcgt ccaaaattat atggcgaagg aaagaatgtt cgtgactgga
    10861 tccatacgaa tgatcattct tcagcagtgt ggctgatttt gacgaaaggc agaattggcg
    10921 aaacctatct gatcggtgca gacggtgaag aagataataa gaccgtgatg gaattgatct
    10981 tagaaatgat gggccaacca aaagacgcat acgaccatgt aaacgaccgc gcagggcacg
    11041 atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga gctaggttgg cagcctgagt
    11101 ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa catgaagact
    11161 ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc caaaaatgga caataataca
    11221 gaggaaattt cactgtatta atggaaaaac acatgtgagg caggcgctcg cctgtctctt
    11281 ttttaaaata aaaatcaagg agcggataac catatgtttt tattaactgg aggaaatgga
    11341 caattaggta cagaattgcg ccatttatta gatgaaaaaa atctgccata tgtttcaaca
    11401 gatgcaaaag acttggatat cacagatgct gagaaaacaa tggcttatat tacagaatta
    11461 aaacccgatg ttattttcca ttgcgcagct tacacagcag tagataaagc agaagaagaa
    11521 gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa gaaatgtagc acttgcagca
    11581 aaagctgtga atgcacaatt cgtctatatc agtacagact atgttttcga tggaaaaaac
    11641 aaaaatgtag aatacgaagt agacgaccaa acgaatccac taaatgaata tggtcgtacg
    11701 aaattattag gcgaaaaagc agtagaagag attttagaag actattatat catacgaact
    11761 tcttgggttt tcgggatcta tggacacaat ttcgtcttca cgatgcaacg cctagcggaa
    11821 acccgcgaca aactgactgt agtaaatgac cagtttgggc gaccaacttg gacacgtact
    11881 ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac catttggtat ttatcatttg
    11941 tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag aaatattgaa agatacagat
    12001 gtagaagttg cacctgttac gtcagaagaa tttccgcaaa aagcaacacg tccgcagtat
    12061 tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg tcataccaac ttggcaagaa
    12121 gcacttgcgc aaatgctgga agctgccaaa aaaatgaata agtaatttcc tttttatcaa
    12181 atagaatttg gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc
    12241 aaaagcgaat caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct
    12301 tattggcgtt ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg
    12361 tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg
    12421 acagaatcgg gaagtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca
    12481 agctgaagag gacttttcgt tggtgaacga ggattattca ttaaacggac aaaacatgat
    12541 cattgcgtat aattctcctg taaaggatat tacggttatc ggaaaaccat tcaactgggg
    12601 attctttttc ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt
    12661 catggtcttg cttggattcg aagtactgat gatattaacg aagaggaaaa aagctctatc
    12721 gctaatcggt gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca
    12781 tgtgggcgat ttgattttct ttactttggg actgatgatt gctttttatc attatttcta
    12841 caatcatgat aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat
    12901 tgggtttatt ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt
    12961 ttattttgtt ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt
    13021 agtgttgatt ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac
    13081 gtcaaaagac gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac
    13141 tggaggaaag tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt
    13201 tgaagatatc aactattcga ataactctga agtggccctg ttctatcact tttttccaac
    13261 tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact
    13321 gggacgtatt ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc
    13381 aaaatggcta gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac
    13441 ttttagtttt gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga
    13501 tgttattcct atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta
    13561 tgcgattacg cattcgaaaa tggataatta tttttcggat tttgaaacca tcgcagttgc
    13621 tgtgttggga acagtgattt tagcttttgc attatttggc tggagacgct tgttttacct
    13681 cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac
    13741 aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc
    13801 tggtaaagtt tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa
    13861 atccttcaat acagtacgct tttatccaga tatagctgca tggaagaaaa tcgatccgaa
    13921 agaagaatac gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga
    13981 agatacaaag ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga
    14041 cgcagaaaaa ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca
    14101 attatatgaa gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt
    14161 taaagtagaa tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct
    14221 agtcgtacag taataggcta gctgagaata atcctgaaat attagtagaa aggagagtga
    14281 agataattca agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt
    14341 tgttgctgac gctagtcttt tttgtttgga cagcaggggt aggttttcca gcaactttgt
    14401 ttatatataa caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa
    14461 atgtttataa agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca
    14521 ttttcttttc ttactttcgg gaattaagaa atgagtcttt atttggagac ttactgtttc
    14581 ctttggttat tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg
    14641 gtatttatct gattgtcttt acggtcttcc tcggaatcac gttatatcgt gtatttacag
    14701 agatccaagt accagaagga caaagcatct gggctccagg caattcgctt tctgatattt
    14761 ggatcaatac gaatacaatc ggaagttctt tgatgactct tgctttattg atcagtggat
    14821 ttgccagttc ttttgaacga tggtatgttc gaatcatcgg tgggatagca gtcgttgcag
    14881 cctttttagg tatttgggtc tgccaatcta gaggtgcatt agtcgcgttg attgtttttg
    14941 cgatattgga tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgcct
    15001 acacggttac gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag
    15061 tcaatttgtt taccgatcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa
    15121 aatctgaaca agttttacta ggaatgaaaa catttatctt ccagcgagga aaccaatttt
    15181 taggaaacca caatagttat aattctattt tgaatatcta tggattgata ggatttggga
    15241 ttgctgcttt gctgcttatc ctttttattg gtcgcttaac gttgaaagca gatttatcca
    15301 acgggcagat gacttttatc tgggcctttt ttgcggtgat gatgcagtca tttatggagg
    15361 atacattgac ttcataccca tgggttccga ttatttatat cctgttggct atggccgttc
    15421 atcgatatga tgaaccagaa gttgcggagg aattctatgt tatggaagag gcatatgacg
    15481 atatgactta tgatgataca tcttatgagg aacaaccaga tagcgctcat tcttccagga
    15541 tccaacgcta tcattgatag gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct
    15601 tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta gagaaaatct
    15661 tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa atatggtaaa
    15721 atggcacttg actgtaattc actgaaaatc gcttggagga acacaaatgt ttaaagattt
    15781 gtatttattt atgaaggaca tttatcagaa cagaaaatta ttgctacagt tttcgatcaa
    15841 tgacttcaaa tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc
    15901 gctatttact gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat
    15961 cgatgcaaaa tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt
    16021 ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa
    16081 agtcgtattc aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca
    16141 tttattcttc attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc
    16201 aatgtcttta cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac
    16261 ttggctgacg gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt
    16321 gctgcagttg atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg
    16381 tcttgtaagc atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc
    16441 atttttaagt gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt
    16501 cacgttagta atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc
    16561 agatgtgctg taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca
    16621 aaatcttata atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt
    16681 aagaaatcct atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa
    16741 ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc
    16801 attacgggag tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct
    16861 ttgctagaac taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta
    16921 aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt
    16981 gaatttgctg acatcggaga ccatatttat caacctgtaa aaacttattc aagcgggatg
    17041 ttcgttcgtc tagcttttgc tgtagcaatc aatgtgaatc ccgatatttt gattgtggat
    17101 gaagcattgg cagtaggcga tcttgaattt caactaaaat gtatggaaaa atttacagaa
    17161 atcaagaact cgggaaagac gattttattc gtttctcatg atatcagttc gattcgtcgt
    17221 ttttgtgatc gttcctattg gttgaaaaac ggagaagtcg tcgaatatgg cgatacgatg
    17281 gaagtaacga cgaattatga aaacttttta aagaaaaaat cagttaaaac agtggatcgg
    17341 aataaaaacc aagtagaacg gttcgccgct ccagatatcg tcgacgtgac gaaagcggaa
    17401 ttgttggata aagacaaaaa tccggtggat atgctggaac aaggagaaaa actatacgtt
    17461 aaagtaactt atgaagtaaa agatgataca atcaaaaatc cggttttagg aattgcgcta
    17521 cgaacagtcg acaattttta tgtatgcgga cttaatactc tactagatgg tatcaagatt
    17581 ccttggaaaa aaggagagaa tatcttttat ctggagtatg aaaagttagg tttgttagct
    17641 ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatat
    17701 aagacaaagt acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc
    17761 ttagaccata aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct
    17821 agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt
    17881 agagtttggt cccggaaacg gccgattaac gaaacattta atcggagcaa aacaatgtga
    17941 agtgagcatt gttgagttag ataaggaatt gttcgatttt gtcagcgaat ttgctcaaga
    18001 tggattttat ggtgatatcg aaagttttga gtgggcaaac tattatgctg gacagacatt
    18061 tgattacgtc ttgtttgcgg atgttttaga acatttggta gatcctggaa aaacattgaa
    18121 gaaagtcaga gaatttttga atgaagaagg agagatcctg attacattcc ctaacttggc
    18181 gcataattct gtcatgatcg atttgttcaa taaccaattg ccgtgggctt cttatggtct
    18241 cctagatgaa acccataatt ctttttatac tcatgatggc ttccaaaaag tttttgaaaa
    18301 agcgggatta ttcattaata ttgaagatta tttgtactta gctgtaggtg atacagaatt
    18361 aaaatcaact tatgaagaat tgccggaagc tgtacgctat gatttcaaaa tgcgaccatt
    18421 tggtgaagtc taccaatact ttttctcatt gatgaaacat ccagtagcac aaagttccat
    18481 tgcagagcca caaaattcta attatgtaaa agtgttagaa gtaacccagc aaacgaaaca
    18541 agatgaaaca atccaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag
    18601 tttccccgta tcagaaacaa cagaaagaat ggtgtttcgc ttcgcgcaac aacctagctt
    18661 tatcgaattc agcgcagaag ctgccggaga aaaactaaca tttatcgatt cgaatgctgt
    18721 agtcaaaaca gtaaatgatt gttatttgtt tgatgggaaa gaattaccag agttcgttct
    18781 aacggatatt tctgggaaag aagtgactat ccattgtcac tatcgcttta tcggggaatt
    18841 gacaccaaca atgaaagaat tgttggaaac gattcgtccc atggctgaag tcacaaagca
    18901 gctttctgag aaaaatgacg aattagaaag agaaaatcat catcttcttg aggaaaatac
    18961 aagactagac catacgttaa aaacaaccac aaatcgctat tgcacattgt tggaaagtga
    19021 tgaatggaca ataaaacata gactaggacg acgtaagaaa gaaacttcga aaaaaatcca
    19081 agaaaaagaa ctttctatat gtattgacga gaaaatctgg gatgcagaaa caaaaatatt
    19141 aaaaatcatc ggatggggaa tcgctaattc ggatcgccag cccttgtcct ataagttatc
    19201 agctgaccaa tcgccatttt tcgaagcaat tcctgtttca cgagaagaag tcaatcaagc
    19261 agaacagctc gcaaaaggaa cagaagcggg atttgagtta cgaatccttt gcgagcaaga
    19321 acgttcattc ttagtggaag cagtagctca aaatggccaa tcatggatca ttgaaatgta
    19381 aataatgatt aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc
    19441 gtatttatcg atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg
    19501 gcaattgatg aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt
    19561 gctgaagcaa cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag
    19621 acccaaagcg gtttcaaggt acgtttatca gggaaaatga gaggaaaagc catcttagac
    19681 ttccaaacag cgagacatca aaatggcata gctgtgaaat taaacggtaa atacccttat
    19741 gatgacggca ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat
    19801 tatgcaagga ctcatggcgt aaaaaaagcg atccgtcggg tcaaattgga attgaagcca
    19861 ggatcgatcg actatgcaga gtggatcagt cgtcatgaga aaccagattt gaaagagcaa
    19921 aggaaagaag cacaaggatt tgcgtaccgt ccattgattt ccatacttat gcctgtctat
    19981 aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg tactggatca aacgtatgat
    20041 cattgggaac tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtctg
    20101 gaatcctatc aagcaaaaga tgatagaatc aaagtcgttt tccgaaaaga aaatggacac
    20161 atcagtctgg caactaattc tgcattggaa atagcagaag gagaatttat agcactctta
    20221 gataatgatg atgaattacc gccatttgca ttatatgaag tggcaaaagt gctaaacgta
    20281 catccagaac tggatgtgat ctacagtgac gaagataaaa tcgatgcaga tggcaatcgc
    20341 tttgatccgc atttcaaagc agactggtcg cctgatacat tgatgggaaa taattatatc
    20401 tctcacttag gcgtatatcg aacaagcatt gttaaagaac ttggcggttt ccgtaaaggc
    20461 tatgaaggtt ctcaagatta tgatttggtc cttcgtgtga cagaacaaat ccctgcagat
    20521 catatttacc acattgaccg agtcttgtat cattggcgga cgatcccagg atcaactgca
    20581 agtaatggcg aagccaaaag ttatatctat gattccggag tgaaagcatt gacagatgcc
    20641 ctgtctagaa gaaacatcaa aggaagcgta cgtcctggca gaatttctgg attttatgag
    20701 attgcttatg acgtcttgca agaggatttg gtcagtgtga tcattccaac gaagaatggc
    20761 tatgaagatc taaaaacttg tgtggattcg atcatcgaaa aaacgtctta ttcaaattat
    20821 gaaatcatta ttgcggataa cggaagtaca gatccaaaaa tgcaagaatt atttgcagaa
    20881 tacaagcatc agctgaaaga ccgctttatc gtagaattga ttgacattcc attcaattat
    20941 tctcgaatta acaatttagc agcagaaaaa gcaaacggca aatacttttt attcttgaat
    21001 aacgatacag aagtgattga gccagattgg atgacggtga tggtttctta tgcacagttt
    21061 gatcgaatcg gctgtgtagg agcaaaactg ttttatccag atgatacaac gcagcatgca
    21121 ggagtattat taggaatcgg tggtgttgca gggcatgcat tgaataacta tgatcgaaca
    21181 cattgtggtt atttcggccg attggtcatt gacgtcaatt acttagcagt cactgcagct
    21241 tgtatgatgg tcaaggcagc tgacttcaat gctgttaacg ggtttgatga aacattagaa
    21301 gtagcattca acgatgttga cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc
    21361 tatgcacatc aagcagaatt gtaccatttc gaatctaaat caagaggtta tgaagatact
    21421 ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa tgcaagataa atggccagca
    21481 tatattgcgc atgatccatt ttacaatgat aatttaacaa aagacggaat cggcgacttt
    21541 tcgttgagac cagattaaac tataataaaa agctgttatg ttgtgattcg ctcgcacata
    21601 acggcttttt atagtttttt gtcaaaaaaa ggcagaaggt gccgactcaa aaaaataagt
    21661 cgaggttggg acagaagtgt ttaactccga gaaataagaa gaaattcacg aaaattgctt
    21721 ttcaaatttt tgtgaatttc ggcttattta agaagaagtt gcttctgctc ccaccgttta
    21781 ttcgttttta aagaatgaga caaaagtgtt tcttactttt gtcccacgct cttattcatg
    21841 ctcagagcgc caatagatac aaaatcatag ccaaagtttc acgaacccta gctgtcaaag
    21901 aatcgatatt ttattttaat ataggctatt ttaaaataaa tggtttaagg tattcctcta
    21961 caaaagccac tctatcaatt atcatgggtt taaaataaga agtgatggag ataaccagat
    22021 tgttcttagc gtcaatataa agaacatttc cactattacc aattgctgaa tatatttcgt
    22081 tctcagaatc taatacccac cataaatagc catatctcat attttgtaag tcttctaaag
    22141 cactggttct aggtttgaac atctcgttta tccacatggg tggtacgatt tgttttcctt
    22201 tatacatccc tttatttagg cataatacac caatttttgc cagatcactt gccgataaac
    22261 acaaaccata tcctgccgtc ccgatattta gtggatcaca aaaccagaca ttggttttag
    22321 gagacttatc cattgtaaac tgcttatgtt cttcagctgt ttcagcataa taattgacat
    22381 ggtttggtat ccctatagga tcaaatagat acttattggc aaaatcaaca gttgttagct
    22441 tacttgtctg atacaacaag ccagttaata tgtgtaaaca gacagtttga tagttgaatt
    22501 cgccggttat accttttctt cctcccaata aatctagtga tgtttttgtc caatcttcac
    22561 ttgaacagac ttttgtccat ggatcgcctt tacatttgta gggtgctttc attgttagca
    22621 agtgcttgat tgtaatagcg taaatagttt tctccccgcg tttcacttta tactctggaa
    22681 aataatctaa tactttatca tcgatacttt tgattaatcc ttgctctaaa gcaatgccaa
    22741 tcagcaaaga tacaatactt ttcgttgctg acatgatatg acaagtatcg tcttttttat
    22801 aaccattcca ttcatctgta taaacttcta ccccatcctt atatgcgaca atttgacata
    22861 tatttggttg tcgaaaacca ataaaatcat gcaactcttg tttattcata agatattacc
    22921 tcactttctt ctataagcat aatgtcatta aaaatcgatt tcaatttttt cgattcttca
    22981 cgtaatacag gtattcttat ggactagaca gcgctttggg aagatggatt ctctgaaaaa
    23041 tataaaggtt cactttatga gtggataaaa aaagcagcaa atacttcgac ttctttaatt
    23101 taccaacatt agtgaaaatc gattcatctg gagatttgta atttagagtc cttgtcttga
    23161 ttagaggtgt ataaagcttt ttattagaca tttttaaatt ccattacaat ttgcttataa
    23221 actaggt
//
LOCUS       DACDKJ010000040        23221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDKJ010000040 DACDKJ010000000
VERSION     DACDKJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.040"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(184..1209)
                     /locus_tag="IV328_001770"
     CDS             complement(184..1209)
                     /locus_tag="IV328_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8937907.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1484..3169
                     /locus_tag="IV328_001771"
     CDS             1484..3169
                     /locus_tag="IV328_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8937908.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3183..4466
                     /locus_tag="IV328_001772"
     CDS             3183..4466
                     /locus_tag="IV328_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8937909.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4536..5405
                     /locus_tag="IV328_001773"
     CDS             4536..5405
                     /locus_tag="IV328_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8937910.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5402..6238
                     /locus_tag="IV328_001774"
     CDS             5402..6238
                     /locus_tag="IV328_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8937911.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6264..7331
                     /locus_tag="IV328_001775"
     CDS             6264..7331
                     /locus_tag="IV328_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8937912.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7328..8155
                     /locus_tag="IV328_001776"
     CDS             7328..8155
                     /locus_tag="IV328_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8937913.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8200..9225
                     /locus_tag="IV328_001777"
     CDS             8200..9225
                     /locus_tag="IV328_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8937914.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9222..11405
                     /locus_tag="IV328_001778"
     CDS             9222..11405
                     /locus_tag="IV328_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8937915.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11405..12535
                     /locus_tag="IV328_001779"
     CDS             11405..12535
                     /locus_tag="IV328_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8937916.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12532..13209
                     /gene="pgmB"
                     /locus_tag="IV328_001780"
     CDS             12532..13209
                     /gene="pgmB"
                     /locus_tag="IV328_001780"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8937917.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13293..13820
                     /gene="msrA"
                     /locus_tag="IV328_001781"
     CDS             13293..13820
                     /gene="msrA"
                     /locus_tag="IV328_001781"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8937918.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14021..14707)
                     /locus_tag="IV328_001782"
     CDS             complement(14021..14707)
                     /locus_tag="IV328_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8937919.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14787..16325
                     /locus_tag="IV328_001783"
     CDS             14787..16325
                     /locus_tag="IV328_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8937920.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16445..16912
                     /locus_tag="IV328_001784"
     CDS             16445..16912
                     /locus_tag="IV328_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8937921.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17431..17874
                     /gene="rplM"
                     /locus_tag="IV328_001785"
     CDS             17431..17874
                     /gene="rplM"
                     /locus_tag="IV328_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8937922.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            17890..18282
                     /gene="rpsI"
                     /locus_tag="IV328_001786"
     CDS             17890..18282
                     /gene="rpsI"
                     /locus_tag="IV328_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8937923.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18370..19596)
                     /locus_tag="IV328_001787"
     CDS             complement(18370..19596)
                     /locus_tag="IV328_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8937924.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(19668..19826)
                     /locus_tag="IV328_001788"
     CDS             complement(19668..19826)
                     /locus_tag="IV328_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8937925.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            20518..21237
                     /locus_tag="IV328_001789"
     CDS             20518..21237
                     /locus_tag="IV328_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8937926.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            21239..22081
                     /locus_tag="IV328_001790"
     CDS             21239..22081
                     /locus_tag="IV328_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8937927.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            22078..23028
                     /locus_tag="IV328_001791"
     CDS             22078..23028
                     /locus_tag="IV328_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8937928.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            23043..>23221
                     /locus_tag="IV328_001792"
     CDS             23043..>23221
                     /locus_tag="IV328_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8937929.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGF"
BASE COUNT         7587 a         4023 c         4786 g         6825 t
ORIGIN      
        1 tttcttccct gtggaaaaaa tagtttaaca caaacattat agttatccac agctatctaa
       61 aacagatgga aaataagaaa aattcgctaa aataccggga gggggtaacg agggatgagt
      121 gattgaaaaa agaacagagt aactgactta tcctcagtgt ttctgttctt ttataattct
      181 gttctacatt tcttgttgtt ggatcgaatc tgtgagagta atctcggttg aaagaatgat
      241 tcttttttct tcagggtatt ggtggtggat tagttggaat aaaagttctg tggctttgat
      301 tcctaactga tttgtcggaa tgtctatccg agtcaactgt ggcgaaaata gggaggacaa
      361 taagggatca ctgaatcctg cgatcgaaag atcatcaggt atctgctttc cagattgttc
      421 tgccactttg taaatactca atgattttaa tgcatcggta gaaaaaatcg cagttggtct
      481 ttctcgtagc ttcattaaat cattcattgc tgccatgcta tcttctaagc gaaagccgcc
      541 gtctataatc cattcattac ggatatctag gtggtgatca aataagcatt gacggtaacc
      601 attgagacga tcattggata cataaacttc tgtcggagca tggagacagc cgatatgacg
      661 gtgtccattc ttgatcagtg tttccaccaa atcgtaacta tcctggaaat tatccgtgtc
      721 cactgagtag atgttcgtgt agtcgtttcc gacttttccg attaccacaa taggaatatt
      781 atattgatcc aattcaaaaa gaagtgtttc atccatcgta gagcttaaaa taataattcc
      841 tcggatcatt ttttcttgga tctttttcgc gcatttttct agctcttctt ctggtgtttt
      901 tgccgtttgt agaatcaagt cgaagttatt ttgttcagaa acggcagaaa tggattgaat
      961 aacatctgaa aaaaaaggat tgccataggt cgtatgagaa gtacgatgag ataatgctag
     1021 aatcgcgtca aaaccatttc ctgacagtcc tcttgcaatt ttgctcggtt cataattcaa
     1081 ttctttgatt gcttgaagta cacttgcttt tgctttatca gagatatttg gctggttatt
     1141 taatactctg gagacagttg attttgagac attagctttt ttcgcaatgt cataaatagt
     1201 aggtttcaat tttatatgca cctccttttt gggagcgttt tagaataatt ccatagtaca
     1261 atagacattc ttttttgtct atgattttga aaatttagga atagcttgca tgatcatgag
     1321 agaccatatt tttttaagaa gctattaatt attgatacat aagctttctt atttgtattt
     1381 ttgtgtatat caaaattatt taaaaaaatg ctattgacag taagcgtttt ccataatatg
     1441 atcaaatcgt tgggaacgct cccaaaaaag aaaggggaat ccgatgtata caaaactttt
     1501 acaagcgatt tatcgcgact cagatttgtc tgagaaaacc gcctctatga ttgaacaaaa
     1561 agtcagtgtt tatcagcaga agaaaggcca ggcaggcaaa ctgcattggg acgaagaaga
     1621 tattttcttg attacttatg gggatcaatt ttttgaagag cagcaaccaa ctttgcgtac
     1681 atttaagaaa ttttatcaaa agtatttggc agatacattt agagttgtac atttcttgcc
     1741 atttttccca tatacatctg atgatggttt ctccgttgta aattatcagg caatcaacga
     1801 ccgtttgggc acatgggaag atatagaaga aatgagaagc gaaacacggt taatgttcga
     1861 ctttgtctgc aatcatatgt ctgccgaaag tacttggttt caggaatatt taaacttgaa
     1921 tgatacgtac aaagattttt ttattgattt gccactggat acagatacaa gcagcgtgac
     1981 tagaccaagg accagcccgt tacttacaac gttcattgac aaaaacggga aagaaaaaca
     2041 cgtatggacg acttttagcg aagatcaagt agacttgaat tttaaaaatc cagaagtttt
     2101 attgaaaatg atcgatattc ttttgtttta cttagaaaaa ggcgcttcat ttattcgctt
     2161 ggacgctgtg ggatttttat ggaaagaact aggaacttct tgtatacacc tgccagaaac
     2221 gcataaaatc atccaattat tccgtgcaat cgctgatgaa gcagctccag gaacggtatt
     2281 gatcactgag acaaatgtgc cacacaaaga taatatcagt tattttggga atggcgagaa
     2341 tgaagcacag atggtctatc agttttcgct accacctttg acccttcaag cgattcgttc
     2401 gggtaataca agtaagttga aagaatggtt aaaaaccatc gatttcgcat cgaaaaaaac
     2461 aacttttttt aattttttag cctcccatga cggtatcgga ttgaatccgg tacgaggtat
     2521 tctgccggaa aaaacgatcc ttgaattagt ggaacagctg aagaaagagg gtgcccgagt
     2581 aaactacaag aaaaacccag atggaacgac cagtccatat gagataaacg ctacgtatct
     2641 agatgcgtta agttctccaa cagatacgac ggaatggaga agcaaacgct ttttagctgc
     2701 tcattcattt ttggtaacgt taccaggcgt gccggcaatt tatattcaaa gtgtgctggg
     2761 aagtcgaaat gattacaaag gcgtagaaaa aacaggagag aatcgaaaaa tcaatcggca
     2821 aaaatttttg ttagcagata ttgagaaaga gttgcagaca gatccattaa gaacagcagt
     2881 atatggtgaa ttgacccaga tgctatctat tcgaaaaaaa gaaaaagcct tccatccgaa
     2941 tagtttgatt tacttactgg actcagcaga ttcttttatc agttttattc gcgaagcgaa
     3001 ggaagagaaa gtcttagtta tccataattt ttccaatcaa tcagcaggtt atcttttacc
     3061 aaaaggagat tacctagacc tgttttctgg acaaattatc cattgcagtc aagaacgaaa
     3121 gttgatattc cagccgtatg aatatatgtg gctgaaaata ataaaataag aggtggaaaa
     3181 aaatgaaaaa aattattgtt actacctgtc tgctagctac tggatttctt cttactgctt
     3241 gtggtgaaag tcaaagctct aacgaatcac aacagacaac gattcaattc atgcactctt
     3301 ctgtcgaaca ggaacgatta agtgtgatca atgaactggt tgctgatttt gaaaaagaaa
     3361 atccagacat caaaatcaag caagtgccgg tagaagaatc agcgttcaat acaaaaatcg
     3421 ttacgttagc tcgttccggt aaattaccag aagtaatgga agtcagtcag gattttgcta
     3481 aagtgatgga taaagatgaa ctgattgatc agacggcagt ccaaaacgtt atcgaggaag
     3541 ctggagaaga taattattat gacggagcca aaaaccttgt tcgttcagaa gatggaaaaa
     3601 gctatattgc tgcaccaatt agcggctggg ttcaaggtat ttggtatgac aaagaaaaat
     3661 tatcagaagc tggtttttca gaaccagaaa attgggatga tattttaaaa attgctcaac
     3721 atttcacaga taaagagaat aaacaatatg gaattgctat gcctactgct gaaggaacaa
     3781 tgagtgagca agcgttctct caatttgctt tgtcaaatgg agcaaatgtt ttggatgatg
     3841 aaggcgaggt aacgatcgat acggaaaaga tgagagaagc attgtcgttt tatcaaaact
     3901 tatcccaata cacgatgcca ggctctaatg atgtgacaga aatcaaagat gctttcatga
     3961 atggaacagc accaatggca atctattcaa cttacatttt gccttctgtt tatgaagcgg
     4021 ggaactcaga aaatatcggt tttgctatcc caacacaaaa agatcaagct gtctatggca
     4081 cagtatctgg attgacgatt tcttctggtc tagacgagaa acaaaaagag gctgctgaag
     4141 cattcacagc tttcttatct caaccgaaaa acatggaaaa gtgggtcttg atgtctcctg
     4201 gtggtgctca accagtgaat aaacaagtag tcgaactgga tggttacaaa gaaaatgaag
     4261 tcatcaagtc ttttggcgag ctgccatctg agattgctag tgcttttgat gaagtacaag
     4321 tctttggact agtcggggaa aagaatttca cgaaaatggg agatatcaca agctcaggtg
     4381 tgatcggaaa agcagtgaac caagtaactg tcggaaacga agatgtcgat caagctttag
     4441 ctgacgcaca aaaaaatacg aaataacaag aagttctgcc aatggtgttc gaaaaaagta
     4501 gatagctatt ggcagattat cagggggagg tttcaatgaa tcgtccaagg aaacattctg
     4561 acttaaaatt agccatggga cttttagcac caagcatgct tttattagct gttttagtgc
     4621 tgattccaat gctttccaat attcagatca gttttttcga tatgccgctg aatcctaact
     4681 cagacgccgt ttttatcgga ttaagcaatt atcttgaaat tttaagtgat agtgaatttt
     4741 atcgttcttt aggtttaaca gttgcttaca cattcttagt tgtagcaggc agtacagctt
     4801 taggattagc cgtagctatt ttgcttaacc gcaagttcag atttaggaaa acggtgcgtt
     4861 cactgatttt attaagttat gttacgccat ctatttcttt gatttttgct tggaaatata
     4921 tgttcaataa tagttatgga atcgtcaatg atctgttagt agataggttg cagcttttca
     4981 acgaagcacc gctgtggttc gaccagccag ttgctagctt ctttatggtc gttttgtttg
     5041 ctatctggag atactatccg tatgcgttta tttcattttt ggctattttg caaacagtcg
     5101 acaaaacgct ttatgaagca gctgaaatgg acggtgcaaa tgcttggcag aagttcaaga
     5161 tcgtgacact gccggcaatc aaaccagtac tagcaactgt gattgtttta cgatctatct
     5221 ggatgttcta tatgtttaca gatgtctatc tactgacgaa taaagtcaat atcttaggcg
     5281 tttatttgta tcagacagcc tttgcgttca atgatcttgg aaaagcagca gctatatcga
     5341 ttctattgtt catcattatt gcagcggtcg tcttgctctc acgaaagaag gtggacttga
     5401 aatgaaaaat aaaaagagca ggaaaatctt attttacgcg acgattttgt tgctattagc
     5461 tgtcaatttg tttccatttt tgatcatgct gatgacatca ttcaaggatt cacaagaagc
     5521 aattgcaacg acacaaacta tttttccgaa agaatggact tttgaacatt atcaggatat
     5581 tttcaatgct gagatttttc cattcatcat gtatttcaaa aatagtttag tgatctcctt
     5641 gatagcctcc gtaactgcag tagtgattgg gatcagcggg gcatacgctt tgtcaaaact
     5701 acgcttcaaa gctcgctcga aaatcaatgc tagcttttac accgtttata tgttttccgg
     5761 tattttgatc gttgttccat tatttaaaat catttccggt ctaggtttgt atgataccaa
     5821 tacagcactg attatcacga tggtcgttca aacacttcct actgcgatat tcatgctaaa
     5881 aagttatttt gacacgattc cgactgatat tgaagaagca gcaatgatcg atggtcttag
     5941 ccggatccag atcattattc ggatcattat accattggct atttctggaa tcatttctgt
     6001 cttcgtttat tgctttatgg tttcgtggaa tgattattta tttgcttcga ttttcctgtc
     6061 gacctccgaa aaatttactt taccaatcgg tttgaacaca ttattcagta caccagacta
     6121 catttgggga cgaatgatgg ccgcttcatt ggtgaccgct ttaccagtca tcattatgta
     6181 tgcgttatct gaacgattta ttaaaggtga tttagtcgaa ggcggcgtaa aaggataaaa
     6241 agaaaagaaa gaggaaagaa aagatgaaaa aattagttgc attagaacca agaatagccg
     6301 gattcaaaga ttacgaagac cgtgagatcg aaaagaacga agtacgtatc cgagtaattt
     6361 tcgctactcc aaaaccagga acagaaattg tcgactttag aggattaagt ccatttatcg
     6421 atgaagaatt ttcagaagaa tggcgcctat tcttgccaag agaagaagga agccaacgcg
     6481 gtgtggtatt tggcgaattt caattaggaa atatgattgt cggtacgatc gaagaaacgg
     6541 gcagtttggt gacagagtat caaaaaggag agttagtctg cacttatgga cccattcgtg
     6601 aaacgctgat tacaaacggt gttgataatt ataaactacg taaacttgat cggttggatc
     6661 aatggaaaaa tgcagtatgt tacgatcctg ctcaattcgc tttaggtggt atccgggaag
     6721 cacatgttcg agcaggtgat gctgtagcaa tcttcggttt aggcgctatc gggcaaattg
     6781 cgattcaatt agctaaaaaa gcaggtgcta ctaccatcgt tgggatcgat cccatagcgc
     6841 agcgtagaga attagcagaa aaacacggtg ccgattacac gctagatcct atgaaagcag
     6901 atgtcggttt tcgtctaaag gaattgacag ataaacgtgg agtagattca gttattgaaa
     6961 cgagtggcag tgctgccgca ttacaagctt ctttaatagg gatcggctat ggcgggatca
     7021 tttcttatgt tgcctttgct aaaccttttc cagctgggtt taatcttggt cgcgaagcac
     7081 atttcaataa tgcaaaaatc gtctttgctc gagcatctag tgaaccaaac ccagattacc
     7141 caagatggaa ccgtaaacga atcgaggaca catgttggga attattgatg aacggttact
     7201 tggattgtga agaaatcatt gatccagttg tgccatttgc agaaagcgca gaagcttaca
     7261 tgcgttatgt agaccagcac ccagaagaaa gtatcaaaat gggcgttcaa gtccgaggag
     7321 gagaacaatg aaattcggta cgcaagatca agcatttttc ccaagagaca ttttagaaaa
     7381 atttagattt atcaaagact gtggatttga agaatacgaa atcgacggta agttgttaat
     7441 agaaaatatc gacacagtca aacaggcaat caaagaaaca gggatcccag tagtgacagc
     7501 ttgtaacggc tacgatgggt ggatcggtga ctttatcgaa gaacggcgtc ttaatggtgt
     7561 acgtcaaatt gctgagattt tgcgtgcact aagcgaggtt ggaggcaaag gaatcgttat
     7621 cccagcagct tggggaatgt tcacttatcg attaccgccg atggtttcac cccgaagcaa
     7681 agaaggagac tataaagcag tcagcgaatc acttcgttat ttagataaag tagccgaaga
     7741 aacaggtacg gttatttatt tggagccttt gaaccgatac caagaccata tgatcaattt
     7801 gttagcggat gcgcgtcatt acattgactc taatcaattg aaacatgtgc agatcatcgc
     7861 tgatttttat catatgaata ttgaagaaga tgatatttca aaagcattga ctacgcaaaa
     7921 agagtatgtg ggtcatatcc atttagcaga taatcatcgc taccaaccag gatctggcgc
     7981 aattgatttt actcgccatt ttcaaacgct acttcaaaat gagtatgaag gttatatggt
     8041 atatgaatgt cgtttgcgag gagaaaatga agtgaaaatt tatcaggaat cattgcaata
     8101 cttgaaaaac tgtctggaaa cagcgcgtca tttaaatgat ttagagaaag aatagagaaa
     8161 gaataacaat aagtaaataa gcaaaggaga gagaaataaa tgaagaaagt agcaatcata
     8221 ggcgctggac aagtagcaga aaaagtacat gctgcttatt atcaaaaaag atcagatgtc
     8281 ttagaattgg ccgctgtggt tgatccagtc agccagcgag cagaagactt ttgccgacgt
     8341 acaggatttt ctcatgcgta tacatcagtt acacaaatgc tgaatgagat ttctcccgat
     8401 atcgtgagtg tctgtacacc caatcgcttt cacttcgaac atgtattggc tgccctcgaa
     8461 gctaatgctt cagtgatgtg cgagaaacca ccagctatga cagctgatca agcaagaaag
     8521 atggcagaga cagccgataa aaacgggcag attttggctt atggattcca gcatcgtttt
     8581 tctgacgaag caacaatgat caagaatcat cttgatgttc tcggggatat ttattacgta
     8641 gaagcgaatg cgcttcgtcg gtctggtgtt ccaggctggg gaacgtttat cgacaaagaa
     8701 cttcaaggag gcgggccttt gatcgattat ggcattcaca tgcttgatac agcgatgtat
     8761 attactggat ttcctaaagt aaaaagcgtt cgggcgtaca gcttccaaaa aattggtacg
     8821 aaaaaaaatt cagggaaatt cgggaaatgg gatccaagca aattcacagt agaagatgca
     8881 atttttggca cactggaatt agaaaacggc aagatcatcc aactgaatac ttcgtttgct
     8941 ttgaatatca gagaagacaa actgaatgtg gctttttgtg gcgatcaggc aggtgctaat
     9001 ctgtatccag gcgaactatt cgaagatgta gatggaaagc tggtttcctt acaaaagacc
     9061 acgccagtta atgacccaca tgaactaagt atggctcatt ttgtgaatcg ggcgctcggt
     9121 gaccagactc agatgattgc agatggctgg caaggatatg tcatccagaa tctggtggaa
     9181 aatttatatc gttcggcaga aacaggagaa gttattacct tatgaaagta gaagaaaaac
     9241 agtttgattt agagtatatc aataagtatg cgagtcagat gaccattgga aatggatttt
     9301 ttggtatcag aggaagccag gaggaagact atcggaatca agtaagggga atgtttgcag
     9361 ctggtgtata taaccgacct ttaggagcag aatcagccga gttagtgaat ctcccagatg
     9421 tcatcagatg ggaaataaaa atcgacggtg aacatttttc tcttgagacg atgaaagttc
     9481 ttgcatacga gcgggcacta gatttagaaa tgggagagct taaacgctat gcgctcattg
     9541 aaactgctca aggaaaaaaa gtggaagtca ttacttgtcg tgttgctgat caaaataatc
     9601 tccatcaggc agccctgaaa gtggtcatca agccgttgac cgaagactgc caagtagatg
     9661 tgaaaacggg tatcgatgga cagcaaacca attttggtac acagcagttg gttgaaaaag
     9721 aactgcgagt atttggtgac gatttgatca gtgcatccta ccagacaaca gaaagtaaaa
     9781 ttgatattgg gattgcagct aaattcaata aagcaggtgt attccaagca aaaaatcgcc
     9841 ggatcaccgc ttccttttca gaaaagatca gaaaaaatac gtcctttacg ctggagaaga
     9901 tcatcgtgct ctctacctct cttcagcaaa cagacccgtt taagaaaagt attgcccaag
     9961 cccagaaaag cgttagttat acagaaattt tagagcagtc gatgtcttat tggacagatt
    10021 tttggcatca gcatcggata acgatccaag gcgattcttc tttcgatcag ttagcgatgg
    10081 acttcgctgc ttaccattta cagattatga ctccccgcaa tgatccaagt gttagtgtag
    10141 gggcgaaggg actaactggc gaaggttata aagggcatgt tttctgggat acggaaattt
    10201 ttatcctgcc tttcttttta tatcagtttc ctgaagtagc aaagaatttg ctgctgtacc
    10261 gttatgatcg attagaaggc gcacggcaaa aagcaagaga ttatcactat caaggtgcac
    10321 ttttcccatg ggaaagcgca cacactggtc aggaagagac acccaaattt gctgcattga
    10381 atatcaaaac cggaacaaga caagaagttg cttcagctgc cgcagaacat catatcagtg
    10441 cagaccttgc ttatgccgtt caagagtatg tccaagcaac agaggatcaa tcgtttttag
    10501 aaacaaaggg aaaaacgctc attcatgaaa cagcagcttt ttggctgagc agagctgtaa
    10561 aagtcgatga tcatttagag atacacagag tcattggtcc agatgaatac acagaatata
    10621 tcgacaataa tgcatatacg aactatctgg cgcattataa tgtggcctta gctgttaaat
    10681 atcaagctgg cgatccgctt tttttgaaag aatgtcaaaa ttttttagat aagttatatt
    10741 tgccagtgcc taacaaagaa cagctgattc cgcaagatga cacgtatctc agtaagccag
    10801 ttattgattt gaccacttac aaagaacgtc ctggcagcca gtctatttta cttgattatg
    10861 cccgtagcga agtaaatgaa ctccaagtca gcaagcaagc agatcttgtc atgctgttat
    10921 acttattacc tgagtatttt tctcaaccaa tcgtttctaa gaatttgcat tactatgaaa
    10981 acaaaacaat ccacgactca tcgttgagta aagcgattca cgccatagag gcttgccgta
    11041 tccaagaaat ggaatgggcg tatcatttat tccaagaagc gtgcctgatt gatttaggac
    11101 cagatccgca ttcatcagat gacggaattc atgctgcttc attaggagca ctatggctag
    11161 cagttatatt tggtttttca ggtattacag taacgaatgg aactctttct atttctccaa
    11221 gacttccttc agcatgggaa aaaatttctt ttccttttat ttggaaaaat cacaggttgc
    11281 tatttgtttt gacaaaaaaa caaatagtaa taaaaaaaga gacgaaagaa acattaccta
    11341 tttttgtcaa tggagaccaa taccagctgg aaaaagaact gatcttggaa cggaggggaa
    11401 aataatgcgt attctgattg cgccagattc tttcaaagaa agtctgacag ccttagaggc
    11461 agcacaagcc attcaatcag gttttcaaga agtttttgtt caggctgaat atgatctgct
    11521 acctataggc gatggaggag aaggaacggt ttcatcatta gcagacagtt taaggttgaa
    11581 gcggcaaaca gcagtcgtta ctaatgcatt tggcaaaaac aaagaaatcc agtacgcttc
    11641 agacggtcaa actgcgattt ttgaaatggc agaaatcgtc ggattgaaag atgtcccact
    11701 aaaccaacaa cagccactta ggttatccac gcgaggagta ggagaactga tacgacaatt
    11761 gttgcaacaa ggtgttcgac acatcatcat cggcgttggc ggaagtagta ccaacgatgg
    11821 cggcatcggc atggcagaag gtctaggtta tgctttttac gatcaagata atcaaaaagt
    11881 agaagcactg ggagaaaatc taggtaaaat tgctacagtt gataaaacga acgtgcctat
    11941 tgagctgatt aaagccaaaa tcactgtcat tactgatgtt actaatcttt tatgcggaaa
    12001 agaaggcgct tcttttgttt tcggaccaca aaaaggcttg aaagaaaatg aattagcagt
    12061 ggctgaccaa gccatgcatg atttttatat gagatttgcc cctcaagtaa tcgaggcatc
    12121 gggcagtagt gctggtggtg gaatgggtgc aggactgtta gcatttgctt cagggaaaat
    12181 agtctctggc atcgactacg tattggattt actggagttc gataaacggg tacagcaagc
    12241 agacctcgta atcgtaggcg aaggacgtct agatggacaa agtctaaaag gaaaagcgcc
    12301 agttgggatt gctagaagag taccagaggg cgttcctgtg atagctattt gcggaagtgt
    12361 tggtaaagga agcgaaaaag cagcagatgt tggcatttcg gccgtttttc cgatccttcc
    12421 tgcagctgtg cctttacaag aagctttaca gcaagccact gataatcttc atcgcacagc
    12481 taaaaacatc gcatctttac ttcaagtcgt tcattttcaa ggaggtaatc gatgaaaatc
    12541 aaagcagcac tattcgattt agatggcgtt cttgtagaca cagctcgtta tcattacgaa
    12601 gcgtggctgg ttttagctaa ccagctgtcc attccattca ctgagaaaga aaacgagcaa
    12661 ctgaaaggaa tatccagaac cgagtcacta gagcgattgc tgtcatttgg aaaaatggaa
    12721 cagaaattct cagaaaaaga gaaatcagcc tttgctgagc aaaaaaacaa tctatatctt
    12781 caagcgattc aaaaaatgga tgaaacatcc gttttgcctg gtgcaatagc tgttcttgag
    12841 tacttgaaaa aaacaaacat caagatcgga ttgggatcag caagcaaaaa tgcacgcctc
    12901 attttagaaa aaacgaattt gacaagttat tttgatgtcc ttattgacgg cacgcaagta
    12961 tcaaaagcaa aacctgatcc agaagttttt ctcaaaggag cacagcaatt gaatgtaccg
    13021 ccaaatgcat gcttggtcat tgaagattca gaagctggtt gtcaagcagc tttggctggt
    13081 aacatgcatg tattgggaat aggtgaaaac ataaatcttc cttctgctga atatgtgatt
    13141 cctgatttaa ccgtttttga tcaagtacgt tcgttttggc atttatcaga agccgtatca
    13201 cgtatataat agtttccgtt ttttctgtca caagcaaaac gttatcctcc ttttggaaat
    13261 caaggtatga tgagaaaaaa gggggatttt atatgacaaa aaaagcagtt ttcgcaggcg
    13321 gatgcttttg gtgtatggtc aaaccatttg aagagcagcc agggatcatt tctgtgacat
    13381 ccggttatac tggcgggcat gtaccaaatc caacttatga acaagtaaca acaggcacaa
    13441 cagggcatac agaagcagta gagatcgaat atgatccaga agttatttct tatgatcagt
    13501 tggtggaaat ctattggcag caaaccgatc cgactgacgc attaggacag ttcgctgatc
    13561 gcggtgattc ttatcgtcca gtgatctact ataatgatga agaagaacga aatattgcag
    13621 agcgttcaaa acaagcattg caagacagcg ggcgattcga taagccaatc gtgacaaaaa
    13681 tcgaaccaag aagcgaattt tatcctgcag aagattacca tcaggattat tacaagaaaa
    13741 acaagctcca ttacaatctt tatcgagaag gttctggacg tgcaggtttc cttcgtcgac
    13801 attggggcgt caagaaataa gaagccgaaa aagctactta tggaacaccg tatatcagag
    13861 gttgtaaaac cagcattttg agctaatact gagggtcagc tgattgaaca ccatttagtg
    13921 gagaggttgt gaacctgtcg cctagctcca agtcacaagg atttggtaag agaacactat
    13981 atatcagagg ttgtgacaag tcgtttgtca caaccttttt tcaataccta agatgaagga
    14041 caatcagcaa cgcactgctc aaaaaaggaa tgaaaggcag cttttccaac ttttttttat
    14101 gacagataaa gtaaacagaa aaaacggata ttccaaacag actagcaaga aacaagatct
    14161 gaagcaactc ttctagagac aagccaccac tccaagaaag catcaacaga gtatcgccag
    14221 ctccgagata ggattgacca tagcgaaggc acacaccgcc tacgataaac gttaacagca
    14281 cactttgcca ttggaagacg ccggcctgca accaaaaaat ccataaaatt gcccaagaac
    14341 agaatagcgt gtgagaatgg accatgaagt aaaataaatc catcaacgaa aatagcaaag
    14401 ccgaaagcat ccatatgaac gtcgaaaaat ttcttgcgga agaaaaagta aagaaccagg
    14461 caaaaagacc accacataag gcttctgcca acaaatagta gaagggcaac cgacacctgc
    14521 aatagcggca gcgaaatcgt tggacgatga tcgagagaat cggaatcaat tcataccaag
    14581 aaagcgaacg acagcatttg acacaatgag atctaggata aataatagag tgacctaacg
    14641 gaatccgctg ggcggttaaa cataaaaatg atccgataca gcaaccaatg ataaaataaa
    14701 gtaacacgcc atctccccct tttcaaaacc aagatacgca agcttaaact aaatttttgt
    14761 acatacataa aatttgaggt gaaaaaatga acgtttcaat cggattagat atcggaacca
    14821 cacaaaccaa agcagttgct ttcacagatg aagcccaaga agcagcatcc gcttattttc
    14881 gctacccttt gatccaagaa acacaaggga tggcagaaca agatcaggaa ttgatctttc
    14941 aaggtgtctg caccgttata aaagaagtaa ttaaacagtt gccttcagac acaatcattc
    15001 aatttgtctc cttctcctct gcgatgcatg gcctgatatt gttggatgaa gagaaaacac
    15061 cgttatctcg aatgatcact tgggcagata accgagcagc ggacgaagca gaaaaactga
    15121 aatataccgt gcttggcaaa gaattttatc ggcagactgg gacgccgatt catccaatga
    15181 caccattgaa caaaatcaag tggctgcaaa acagccaacc tgaattattc aaaaaaactg
    15241 cttattttgt cggtatcaaa gactatatct tttatagatt gtttggcgaa ctgatcagcg
    15301 actacggaac agcttcagga actggatatt ttgatatcca tgaattccaa tgggcagaag
    15361 aagtgcttga ctacttgaac attcgaatag agcaattgcc gcaagtcttt ccttccacct
    15421 atcaagtaac aggtctacca tcaaaaacag cagcagaact aggtttgcca aatgatatat
    15481 catttgtttt gggaggggca gacgggccgc tttctaatct agggttaggt gcttttggtg
    15541 atacgatcgc cgctttgacc gtaggaacga gcggagcact tcgttttatt actactcagc
    15601 ctcagttcca tccagaaatg gagacgtttt gttatgttct ggatcagaaa cattgggtag
    15661 tgggaggagc gactagcaat ggtgctggca tctttgattg ggcagggcat acatttttac
    15721 aggaagtcgt tcaatctgct aaagaaaaag gagaaaatcc atatgatgca ctgctttcgg
    15781 ctatcgcttt tgtgccagcg ggtgccaatg gtctgatttt tcatccctat ttacttggtg
    15841 agcgagcacc gctttgggac gcagatgcgt ttggcagttt tatcggttta cagcggcaac
    15901 atacagaaaa agaaatgatg aaggcgattc tagaagggat ctgtctcaac ctttatcgga
    15961 ttttaaaagg agtttgccag gaaaatcagc tcacagagat ccgtgctaca ggcgggtttg
    16021 ctcaatctcc gatttttcgt cagatgatgg ctgatgtact gggttacccg cttgttttcc
    16081 ctactgtcac agaagcatcc gcattaggag ctgtgatttt aggctggaga agcatcggaa
    16141 agatccaaag tatcagtgaa gcacaggaat tgatagaatt gaaagagcaa gttggagtac
    16201 aagaaactga tcataaaaaa tatcagaaaa tctatccatt atttgtttcg acacaaaagc
    16261 atttagcaca gacttatcag caatttgctc aactgcgaga agaattagct gattctgaaa
    16321 aatgattggc tttacggata aaaaagcgaa ctgaaaatcg ttctcaggta aatgattttg
    16381 ttgtactttt cattgaaagt ctactattat tttaagtgta agaaaatgtt aggaggcaag
    16441 aaagatgaaa ttccaacaaa ccaaagaagt attaaatcaa ttagttgcag atctaagcca
    16501 aatgtctgtt gttgttcacc aaacacattg gtacatgaga ggaccaggtt tccttacatt
    16561 gcacccaatg atggatgagt tcatggatga tttgaacgct cagttagatg aagtatccga
    16621 acgcttgatc acattagacg gttcacctta ttctacattg cgtgaatttg cagataacac
    16681 aaaaatccct gatgaaattg gccgctggga tcgtactatt ccagaacgtt tggaaacact
    16741 tgttgcaggc tatcgttatc tagcagatct ttaccaaaaa ggaatcgaag tttctggaga
    16801 agaaggcgac gatccaacac aagatatctt tatcgaattc aaaacagcca cagaaaaacg
    16861 tatctggatg atccaagctc atttgggcaa agcgccagag atcgacgcgt aaaaactgtt
    16921 gttgaacttg accgaaaaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa
    16981 caaacgtcaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagct
    17041 gattgaacac cgtttaccaa gaggttgtaa gcctgccgtt cagctccgag tcacaagaaa
    17101 caattttaaa aaacagcttc ttatgtttta tggatattgt gacttgtgac cgaggagttg
    17161 gcaggagaac accgtttatt caaaataagg ggttgtgaca agacttttgt cacagcctct
    17221 ttttgttttt cattttgaca ggaaacgacc cttttatata aaagaaagaa aagaagtaaa
    17281 gtttcatata aatttttcaa tcaagcagaa aaacgttgac attgactaga ttagcgggta
    17341 atatgttaaa tggtattgtt tgccccacaa agagccccgg aaactcgaag tgtatgtcaa
    17401 acatcagaac ttaaattgga ggaaaataac gtgcgtacaa catatatggc caaaccaggc
    17461 gaagtagaac gtaaatggta tgtagtagac gcaactgatg ttccattggg acgtctttca
    17521 acagttgttg catctgtact acgtggtaaa aataaaccaa ctttcacacc tcatttggat
    17581 actggcgatt tcgtcatcgt catcaatgca gacaaagtga aattaacagg taaaaaagcg
    17641 actgacaaaa tttattaccg tcacagcaac tacccaggag gattgaaatc aatcactgct
    17701 ggcgaattgc gtgctaaaaa ttctcgtcgt ttgatcgaaa cttctgtaaa aggtatgctt
    17761 cctaaaaaca ctttaggccg caaacaattt acaaaattga acgtatacgg tggagcagaa
    17821 catccacatg cagcacaaca accagaagtt ttagacatca caaacttaat ctaataggag
    17881 ggaatttcat tggcacaagt tcaatatatc ggcacaggcc gtcgtaaaaa cgcagttgct
    17941 cgcgtacgtc tagtaccagg aactggcaaa attactgtaa ataaaaaaga tgttgaagaa
    18001 tacattccac atgctgactt gcgtgaagta atcaatcaac ctttcgctgt tactgaaaca
    18061 aaaggcgcat atgacgtatt tgttaacgta aacggcggag gatacgctgg acaatcagga
    18121 gctatccgtc atggtatcgc tcgtgcattg ctagaagttg atcctgactt ccgtttagct
    18181 cttaaacgtg ctggcttact tactcgtgac gcacgtatgg tcgaacgtaa aaaaccaggt
    18241 cttaagaaag cacgtaaagc ttcacaattc tcaaaacgtt aatatccttt atatatcaac
    18301 gtttctggca cttttcaatt atttggaaag tgcttctttt gtttttaaga acacatttaa
    18361 gaacacatat caatcacact tattcaatcc tctctgaaaa gcttcaaaaa cagccttatc
    18421 aactatatct ggtgtgttca catatatctt tgttgtagaa atatctgagt gtgttaaagc
    18481 atttgaaatt tgattcatat ctgctccacc ttgtctagct atagttgcat aagtatgacg
    18541 gagtttgtgt ggagataaat gaactaagtc aggatgcctt cttttaattg aattgattcg
    18601 atagtttagg taatcggcat gaacaggaca atttacttct ccagatggtt tagtataagt
    18661 aaagagatac tgatctaacg tttgcttaat ttttaatttc aacaattgat cagcttgtac
    18721 cgatttccat tcacttagca atgtcattac aacttctggt acttgaatga cggtatcttt
    18781 tcgacctttt gtaaactttt ttctttgata tttatctaaa gtatgtttaa gacggaccgt
    18841 ttgtttttca aaatcaatat atttccattg caaagcgtaa gtctcacttt ttctatcacc
    18901 aagatacagg gtaatcataa aaagtgtgta atcatgaaaa gtaagtaaat gattattaaa
    18961 atcatcattt actgcttcta tccaattagt aagttctttt gatgatagtg cttgcttacc
    19021 ttcttgtatt cttttttctt ctaaagcatt ttttttaggt gcagtgatac tttgaataat
    19081 tttagtagtt cgattaaatt cgatataatt tagtatttct gcaatatcga acatggaacg
    19141 aacatatcct tttatgattt ttatatttgc atattctttc gattttttgg ttaattcatc
    19201 tgatattatc tcagtattga tatttagata attcattgcg tattcaccaa acataggtaa
    19261 aatatgcaac cgaaatatat cttttgtatt actaatggtt atatcactag gaggcctgtt
    19321 tgatctaatt gtttgaccta attcatatct aggaagccat ttagattcgt aaaatttttt
    19381 aaacgttatt tttcctttta attctaatga gtttgcattc tcctctctca aaactttttc
    19441 tattttcttc ttcatatcat tttcggcttt aatcgccaat tgcctatttg gaaatgtttt
    19501 ttgatacttt tctgatgcaa ttcctgttat ttctttaaac ctttttggat atctcagatt
    19561 agttttatat ttcccatttg aaagctttgt aggcaaaatc ctcactcctt aaaaaaagta
    19621 aggcagtata tttcactaat tttagtatat gcggcgagaa gaaaatttca agttatttta
    19681 aatgtattca taaagtcttt aatatcttct acgtcaaagc gaactacacc accaactctt
    19741 attactggga aattatatat acgaatccac ttatctagag tattattatc gattcctaga
    19801 aatgcacagg tttgtttttt attcaggtag actctggatt ctataaaatc atctcctaaa
    19861 atagtagtta tgaaattttt gaattgcttt gggtaagcac ataggaattt cacctctccc
    19921 cagtttacgg cgagccttac ggaagtatca ttatttgctg ctattgtcat ggcattttgt
    19981 tttcccagaa caaaaagaaa tctctaagta tttgtcgcta atcaggtcat ggcgtacttg
    20041 agtttctagc tcgaatagca gggatcgtta ttcaaaaatt gtatttaatt gtcaatgatc
    20101 aaaaatagtt tttttcctat tcactattaa taacgataaa catgtataaa aagacacact
    20161 acgaaaaaag ttaaatggag tttactgatt ttcatatttg tatatggaac agacatagtc
    20221 ttgaataatt tttataaatg ttttgatgct tatcaactta ttcaacccgc tatccacttc
    20281 aagtataatt tttccatacg ccaataaagt agttttagga gcatgttctc atttgagaac
    20341 gctccttttt tatatatttt gaaagctact tactatacgc gtagtttcaa atagttttgt
    20401 cattctaatc ttcagcaaca gaaaaaaatc taaactataa aatttagggt tgactaaaat
    20461 ataatttttg tataccatat gatcgtgata atcattatca tttagaagga gaaagttatg
    20521 ttagaagtga aaaagcttaa tgtttgttat aacgattttt tagcattgaa cgagatttct
    20581 ttaaaaatac aagaaggtgc tttaacaggt attattggtc caaatggtgc tggaaaatcc
    20641 acattgctta aagcaatgtt aaatattatc cctcatcagg gagaggtctc cattaataat
    20701 gaagatatca ataaaaaact ggtgaagatt gcatatgtag agcaaaaagc tgatattgat
    20761 tttacttttc caataaaagt aaaagaatgt gtatctatgg gaacatatgc cggaatgaaa
    20821 gtttttcaaa gaattaaaaa tgcagaatgg caaagagttt caaaggcttt ggaaaaggtc
    20881 gatatgggaa aatattctaa tcatcaaata ggagaattat ctggaggaca atttcaacgt
    20941 gtacttttag caagatgtct tgctcaaaat gcggatttca tatttttaga tgaaccattt
    21001 gtagggatag acttagttag tgaacgaata attatggata cgttaaaaaa attgaaacat
    21061 cagggaaaaa cagtgctaat tgtgcatcat gatttaagca aggtaaagaa gtattttgat
    21121 aatattattc ttttgaatcg aaatctaatt gctcacggaa gtgtagaatc tgtatttaat
    21181 gaagaaaatt taaaaaaagc ctatggagat actatattta ttggagaggg ggaataatat
    21241 gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg ctctaattac
    21301 atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt taagaggaat
    21361 gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa tttcttatat
    21421 tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt ctatcattat
    21481 tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta ttactttcag
    21541 ttctttttta gcactaggag ttatccttat aggagttgca aatagctcaa cggatttatt
    21601 tcatatttta tttggaaata ttttagctgt acaagatatt gataagtgga ttacaattgg
    21661 cgtttcggtt ttagtgttgg ttatagtagt tctatttttt aaagaattat taatcacttc
    21721 ttttgatcca ttaatggcaa aagcaatagg gatgaatgtc agtttttatc attatctatt
    21781 gatggttcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa ctatattaat
    21841 tgttgcaatg ttaattacac cagcagctac agcatattta tatgcaaata gcttgaaaac
    21901 tatgatattt atttctgcaa cagtcggagc aagttcatca ttgcttggat tgtttattgg
    21961 ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat tcttatttgt
    22021 aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga gatcgttatg
    22081 atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct ttttatattc
    22141 ggggcatgtt caataaatag taaagacaaa gataaagtgg cttcgaacga aaaattaaaa
    22201 gtagtagtta ctaattcgat tttagcagat attaccgaaa atatagcaaa agataaaatt
    22261 gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc tttgcctgaa
    22321 gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa cttggaaact
    22381 ggaggaaatg cttggtttac aaaattagtt aaaaatgcga acaaagagga aaacaaagac
    22441 tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag tgagaaaggg
    22501 aaggaagatc ctcatgcttg gttaaattta gaaaacggta ttatttacgc taaaaatatt
    22561 gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga aaatctagat
    22621 aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt tgcttcaatt
    22681 ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt ctcgaaagcg
    22741 tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg aacaccagat
    22801 caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt attcgtagaa
    22861 agcagtgtgg atgatagacc aatgaaaaca gtatcaaaag ataccaatat tcctatctat
    22921 tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag ttactatgcg
    22981 atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt tggagggtag
    23041 gtatggtaca aattaaaacg actactatac taatttattt actagcaatt caggaattat
    23101 caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt gttagtccag
    23161 catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat ataggatttc
    23221 a
//
LOCUS       DACDKJ010000041        23074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDKJ010000041 DACDKJ010000000
VERSION     DACDKJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.041"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            101..2542
                     /locus_tag="IV328_001793"
     CDS             101..2542
                     /locus_tag="IV328_001793"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8937930.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="IV328_001794"
     CDS             complement(2872..3576)
                     /locus_tag="IV328_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937931.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="IV328_001795"
     CDS             complement(3763..4008)
                     /locus_tag="IV328_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937932.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="IV328_001796"
     CDS             complement(4025..4876)
                     /locus_tag="IV328_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8937933.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="IV328_001797"
     CDS             5042..6046
                     /locus_tag="IV328_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8937934.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6287..6643)
                     /locus_tag="IV328_001798"
     CDS             complement(6287..6643)
                     /locus_tag="IV328_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8937935.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7011..8198
                     /gene="tuf"
                     /locus_tag="IV328_001799"
     CDS             7011..8198
                     /gene="tuf"
                     /locus_tag="IV328_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8937936.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="IV328_001800"
     CDS             complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="IV328_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8937937.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11003..11722)
                     /locus_tag="IV328_001801"
     CDS             complement(11003..11722)
                     /locus_tag="IV328_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8937938.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12042..12563
                     /locus_tag="IV328_001802"
     CDS             12042..12563
                     /locus_tag="IV328_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8937939.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12622..13482
                     /locus_tag="IV328_001803"
     CDS             12622..13482
                     /locus_tag="IV328_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8937940.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13589..15982
                     /locus_tag="IV328_001804"
     CDS             13589..15982
                     /locus_tag="IV328_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8937941.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16164..18005
                     /locus_tag="IV328_001805"
     CDS             16164..18005
                     /locus_tag="IV328_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8937942.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18029..19138
                     /gene="rpoD"
                     /locus_tag="IV328_001806"
     CDS             18029..19138
                     /gene="rpoD"
                     /locus_tag="IV328_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8937943.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19358..21457
                     /locus_tag="IV328_001807"
     CDS             19358..21457
                     /locus_tag="IV328_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8937944.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21653..22516
                     /locus_tag="IV328_001808"
     CDS             21653..22516
                     /locus_tag="IV328_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8937945.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22621..>23074
                     /locus_tag="IV328_001809"
     CDS             22621..>23074
                     /locus_tag="IV328_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8937946.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7769 a         4038 c         4810 g         6457 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc
     6181 taaacacgta taaacggcgg aagcagaagc aactccttct tatttcttgg agttaaacgc
     6241 ttctgtccca gcctcttttt atctaataaa attttaagac catttactaa ttatcgattt
     6301 tttctgccgc atttttaggc acttcagaag cctttatttc ttcccagctt aatacacctt
     6361 tacgaggatt gactttgagt ttaagatacg cattcatctt taatggatgt tctctttctt
     6421 cgcgaagcgt cacctgtttt ttttcaccat ctttattata agccgcttgc ttgtaggtat
     6481 aagtcgtgaa tttctctccg ttatctgctt gggtagctga catttcgcca ttcgtcgtga
     6541 tttttgtata ataagcttcg ccgccgtaaa aataatgata agctgcaaaa cttccgccga
     6601 gaaatatgcc taaaacgaca agtacgccaa ttaatttttt catgtacttc acctctttcc
     6661 ttattattaa cgaaatagaa gatttcgtct attgaaagtt gttggcaaca gctgaatttt
     6721 aaacttttcc tcattttgtt cttattgctt caaaagaaag ttaaggacta tcttgaagtt
     6781 tttctcaaaa aacacatttt tttatttata aaaggtactt ttttcgcgta taagacagac
     6841 gagtgctctt agttgacttt gaccaatatt gacgtataat aagcttagtt cgaatcttac
     6901 aaaaagtaag tcggacgtat tctttttgat ttagtcaaaa aaagactata ataaaaaggt
     6961 gagagctgaa atttttcagt gatctatatt atttaggagg tttgtaaatt atggcaaaag
     7021 aacattatga cagaagtaaa ccacacgtta acattggtac gattggacac gttgaccatg
     7081 ggaaaactac tttaactgct gcgatcacta cagtattagg caaaaaaggc ctagcaaatc
     7141 ctcaagacta tgctagtatt gatgcagctc cagaagaacg tgaacgtggg attacgatca
     7201 ataccgcaca cgtagaatat gaaacagaaa aacgccacta cgcgcatatt gatgcaccag
     7261 gacacgcgga ctatgtgaaa aacatgatta caggggcagc acaaatggat ggtgcaatct
     7321 tagttgtttc tgcaactgac ggtccgatgc ctcaaacacg tgaacacatt ttattgtcac
     7381 gccaagttgg tgtaaaatac ctgattgttt tcttgaacaa agttgattta gtcgatgatg
     7441 aagaattgat cgatttggta gaaatggaag ttcgcgagtt attgagcgaa tatggtttcc
     7501 caggcgatga cactcctgtg atcaaaggtt ccgcattaaa agcattgcaa ggcgatccag
     7561 atgctgaagc tgctattatg gaattgatgg atacagtaga tgaatatatc ccaacaccag
     7621 aacgtgatac agataaacca ttactattgc cagtggaaga cgtcttctca attacaggtc
     7681 gaggaactgt tgcctcaggt cgtattgatc gtggtgctgt tcgtgtcggt gatgaggtag
     7741 agatcgtagg gatcaaacct gaaacacaaa aagcagttgt aacaggtgta gaaatgttcc
     7801 gtaaaacgtt agattacggg gaagctgggg ataacgtagg cgtgttgtta cgggggatcc
     7861 aacgtgacga tatcgaacgt ggacaagtac ttgctaaacc aggttccatt actccacata
     7921 caaaattcaa agcagaagtg tacgtgttga caaaagaaga aggtggacgt catactccat
     7981 tcttcaacaa ctatcgtcca cagttctact tccgcacaac tgatgttaca ggaacaatca
     8041 cattgccaga agatacagaa atggtcatgc ctggtgacaa cgtgacgatt gatgttgact
     8101 tgatccatcc aatcgctgtt gaaaacggaa cgacattctc aattcgtgaa ggcggacgga
     8161 cagtaggttc aggtatcgtt acagagatcg aagcataaga aacgcgactc gaatattttg
     8221 gtaatgaaaa atgtttaaaa taaaagagcg gtccaaacag gatttctgtt tggaccgctc
     8281 tttgttcttc aattgtataa gaagagattg tagaagcatg aactttcgtt ttacttcagt
     8341 ctcttttatt catcatgaga gtgttcatct atcggtatgc agtttttaat actttcttcg
     8401 tatccatttt ttcaaaccac ttattctacc ttgtgtctgc tcaactggcg cattatcaat
     8461 aatcagcaaa gcaatcataa catatgcaat ccaactatct ctagaatata gagtataacg
     8521 tgagacccag tttgtagcgt acttgctttt atattctctt aatccatgga aagaataaaa
     8581 atgagaacca aactcgtaaa ccaagtatgc aatcctttcc tgtaaaaaac tctttctgga
     8641 agttccgaca tttgaaagag gggccatccc taaattgaac cattggatat gttcttcttt
     8701 catataggca aacaaatgca agaacaagaa atccatactg ccagaaggtg cttttttaga
     8761 atcatatcgc atcagatcaa tcgttcctac tttattgttc gtgtaagtag gcatgatcgt
     8821 tgcaaaagcg acaatttctt cttgggtatt tttgacaact gcaataggcg ctctttgtag
     8881 ataatcttca gagaaaaagc ctaaagaaaa ccctttttcc tttcgtgaac ccaaccaatt
     8941 atcagagata tttttgaaag tactcatttg ctcagtagaa aatggtggtt gtaagacatc
     9001 aaatgtaaat ccttctcgtt cgatccgatt caagacagca cgagtgccct tcattttttt
     9061 ccctgaagtt gtaaaactat tcaggtctac ataagcttct tcccccatct taatgaaatc
     9121 gtagccgaat tcatgcaaaa tcataacaat ctcttcgctt gtttcgtaga aaactggtaa
     9181 atagcatagt cgatctgttt cttcgataaa agcttcgatt gcttctggaa aatcttcttt
     9241 cttaccagaa ggatcaccca ttacaataca tttattattg aagcaagcga attggagaag
     9301 aacagttggt tcaccgtctt tttcataggc aaacatccgc ttatctttta agaagatcaa
     9361 ttgactatcg gagtttcctc cataagttgt aaggatcttc agggcctttt cttcattaaa
     9421 ggcttcgcca atctgtttct tttctccttg aaggaaatga acaaatagaa cgatcatgaa
     9481 actaacagcg atgatggcca acaatcccga aaaccaaatc ttttcagaag gaaataagaa
     9541 aaaggaaata aaatggtgac gccgatgagg gaaatccggc agattgtata ccccaataac
     9601 gatgtaaagc aaggacagtg caccaataat gattccatcg atcgtacgcc actcccatga
     9661 atagatcaat tgttcccgaa ataattcgtt tttagaagca agaatgatca gaagcagaat
     9721 agacaagaaa accaccggtg tgaaactaaa atcacttaat aaaacataaa gtaaagctag
     9781 aacaatcaag aagattgtag gcaaatatgc tcgtttcaca cgtgctgcga tccctctgcc
     9841 cataatgatt aaaagaaatc ccaatagtat actaggaaat tggataataa aatgaaattt
     9901 caatgggttc aaactatgaa gccagcgaaa ctctgtaaaa gcttgcggga tagtggcaga
     9961 aagtactagc atgattccag aaaagtataa taagacaacc acgattttat gcgcaatttc
    10021 tgttgctaat tgtttaggga taccagaata acgctgatca aaagtggaac cgatattttt
    10081 gaagaaaaag acgattccaa tcaaaaacgg aatgatgtag tagaacaggc gatagagcaa
    10141 gatccacaca acgactattt ctcttggcac accaatagcg gacaacccga taatcatcat
    10201 tacatcgaaa ctcccaattt ctcctggaat catagacacg attcctatca ctgaagcagc
    10261 aatgaataat ggaattgttt gccacagatc aatccggatt tccattaaat aaccaacaga
    10321 aaggaaacta aacaggacac cggtccattc caacaatgaa actagcaata gctgtccttg
    10381 agttttaagt gaggtaccac caaggaatcc tttctttttg aaagtagtaa ataaaaagac
    10441 gacaggaaaa tatagaccgc cgccgatcaa ccatatccaa tattgcttca aaaaaggagc
    10501 aactggcgtg aagactacaa gtaaaaaaga aagcagactg taaatagaaa gtccagccat
    10561 caaaaacaag aaaactttcg ataatgcttg gatcacgttt ctgctctctt tcttttgtcc
    10621 atatagttca gagcgaagcc ccacactaac aaacccgccg aaaccggcaa tattgttgat
    10681 cgtattgatc agccagcttg tctcaaacag gtaacgagga ttctgctttt gttcaagcat
    10741 ccgattcaaa ataatatcat aaccaaccat aggtaacaca gagataagac caatcaatag
    10801 cattagtccg cttttccata caggtatagt ggcaaacgtt tctcctaatt gctggataga
    10861 tagagtttta ccgatggaca tcaattcacc gacaataatg acaatgacag aaatcaaaaa
    10921 gattgttttg aacagtccta ggtggttttt cagccattgt atggtatttt ttaacaaata
    10981 aatcaccttc tattcctaat agttatgttt cagaaagaaa ttgaagaact tctcgattga
    11041 agcgttcagg atcttttcta gctaattcat gtccttgttc tttcacaagc acaaaggaag
    11101 cttttggtat agttttagct atatacaaag aatgttttag cttgattaca tctttttttc
    11161 ccacgatgat caaagtgggg gagttgattt tttttaaatc attttctgta agcccgatgt
    11221 catgcagcaa aagtttgata actaaaagat ttttgcgaag acttgggcga aataaagaga
    11281 gtaaccatac ccatgcataa tgaatattac tgatgactct ggcagaaaaa cgaacgcctt
    11341 taacaagcgt gtttccagaa tttaaaatca atcgatgaac ttttttaggg aagctgctgg
    11401 caaataccag tgctagattt gctccatcac taaatccaag aaaatctgcc tgatcgattt
    11461 tttccagcaa catgatcgta ttcagatcct cggccattaa ctgaaaattc aacatagatg
    11521 cttcgtttgt cgaacgacca tgtcctcggc tatctactag atacacggta tagtagcgtt
    11581 cgagtacagg gacctgttct gaaaaaaagc ggccgctgcc gtcattgcca tgcagtaaaa
    11641 agagagggaa gccttgaccg ctcttttcat agtaaatttc gctcccatct ggcatttttg
    11701 catatttctt ttcttttttc acataatcac ctactgtgta aagtataacg attatatcag
    11761 tcattgggca attgttttta ctgaaaaaca acgatcattt tcgtaagatg tcaaaaaata
    11821 caggaaaatt cattttttct taaggcgtac acagaaatca aggaagaaat agccaagaag
    11881 ataaagaaat agtaagataa aaaaagatgt caaaataact aggtgatgaa atgaagcgaa
    11941 aacgattatc tattatccgt attctttttt acatgatcat tctgacggtt attggtgcgg
    12001 tgttgttttt atgaggaaga acttatcgtt cggtaaaacg agtggaaagc tatcaaatag
    12061 aaataacaac agctgcaaaa aattatcata tggaaaacta cgagaatttg atcaaggcga
    12121 taatattgac cgaatcaaaa ggaaaaggga cagatctcat gcagagctcg gaaagtctgc
    12181 atggagaagc tgagatcatt gataatccag ctgacagtat tgatcaaggt gtcaaatatt
    12241 tggcggaaat gatagtagaa gcaaagaaac aaggatgcga tttatggaca gctgtccaag
    12301 catataattt tgggaaagat tatattgcct atgtaaaaaa tcatggcgga gaaaacacag
    12361 tagaattagc agaagcgtat tctaaagatg tgctgagccc acttttagga aatgaggaag
    12421 ggaaacaata ccgatattgg cggatccagt ccttgcttta caatggcggc tatctctatc
    12481 ataatggagg taacatgttt tatcctgata ttgtcaaaat gaatgaaaaa ataatagaga
    12541 cctatgaaaa agtctttaag taaaatagca ggaaatgttc aatcatgaat aaaattaaat
    12601 gaaagaagga gtgcgcaaat agtggaaaaa aggcaaaaaa tggaaaatca agaagtgggg
    12661 tacacaacca aagactggat gttacggttt gtgaagggaa tgtttatcgg ttcaggattt
    12721 atcttgccag gagtcagcgg tggtgcatta gcagctgtct ttggcatgta tgagcggctg
    12781 atttcttttt tagcccacct tacaaaaaat ttcaaagaaa atgttttgtt ttttctgcct
    12841 gttggtctag gcggagtgac aggcatcttt ctactttctt ttttagtcag ttttttatta
    12901 ggtgcgtatg aaacaacgat attatggttt tttgtcggat gtatcgctgg aactgttcct
    12961 gcgttatgga aagaagcagg gaaaaaagga agagatactt ctgatgtgat catcatggtt
    13021 gttacttttg ttctggctta tttgttttta ttatacggcg ctcaattatt tgacgggcaa
    13081 gtcgatcaga attttggaac atggctgatt gccggcggat tgattggttt gggtatgatc
    13141 gtcccaggat tgagcccttc taattttttg gtgtatatgg gattgtataa agcgatggct
    13201 gacggattta aagaagtacg acttgaagtg attgtgccga tcggaattgg tgggattctt
    13261 tgtgttcttg gattgtcaaa aatcatggat tacattttct caaaggcata tagcaagcta
    13321 ttccatttca ttttaggtgt agtttttgct tcaacgatta tgatcattcc taccgattac
    13381 gcgggattgg gagttgctgg ggtactagct tgtcctgttc tttttatagc cggtacagca
    13441 ctaggttggt ggatgagccg tttggaggaa caatataagt aaagatagat tgataataaa
    13501 aacagcacac gggtgattca aacaaaaagg ttggcatctt ttttgtaaaa tcattcgtgt
    13561 gtttttctat aaattttata aaaacaagtt gtttttaatc ttggaacacg ctagacttaa
    13621 acacgtgatt atttttgaaa gttggcggaa aatggagaaa aagaaaatga atggacttac
    13681 acagcaagag gtagttcaga aaatggcttc tggccagcaa aatgactacc aggaagatgc
    13741 atcaaaaagt acaaaacaaa tattcagcga taatatattg acgttattca attttctgaa
    13801 ctttggtatt gggatttgtc tgttatttgt aggtgcgtat tccaacatgg cgtatttagc
    13861 aattattgtc gtcaatatcg ttattggaat ttatcaagaa atacatgcaa gaaatcttgt
    13921 acggaaatta tcgattgttt cccaatcaga agtactagtg atccgggacg gtaaagaaca
    13981 atcgatttct actaaagagc tggtattagg agatttggta aaaatcggag caggagaaca
    14041 gataccagcc gatttacagg ttatatctgg acgggcagaa gcaaatgaag ctcttttgac
    14101 aggcgaatct gatttgattg tcaaagaaac tggagctgaa ttgctttctg gaagcttttt
    14161 gacaagcggt tctgtcatgg ctgaagttat ccgagtaggt gcagataatt atgctgtccg
    14221 cttgactagc gaggcaaaga ctcacaaagc tattcgttct gaacttgtag attcgattcg
    14281 caaagtatcc aaattcacta gtttcgtgat tattcctttg gggatcatac tatttttgga
    14341 agcactgttc atccgcgatg cttctatcaa aatttctgtt attgcatctg cagcagcttt
    14401 gttaggcatg cttccaaagg gtttggtatt gctaataagt attgcattaa cgacaggggt
    14461 aaccaaactg gcaaaaaaac gaatccttgt tcaagacatg tattcaattg aaacgctggc
    14521 ccatgtcgat acattatgct tggataaaac agggaccatt actgaaggga aaatgagtat
    14581 ccagcgggta gaagcactac atccgctgta cgagaaagcc atcccagaaa ttatggggac
    14641 gtatctggca gaaagcagtg ataacaacat cactatgcgg gctatgagag attattttga
    14701 gctttctaat cggtatgatg tgacaaacaa agtcgcattt tcttctgatc gcaaatgggg
    14761 cgcgatggaa tttccagaac tgggagtcgt gtacgtagga gcgcctgaac gtttggtgga
    14821 agaagatcgg cttccggaca gtctaaaaac agcccaagcg aatggctatc gcgtacttat
    14881 gttggggatc gctccaaatc agcaattgaa cgaagaaaaa ccagcatttg ttgagccggt
    14941 tgctctgttt gaaatcgatg atccaatccg aaaaaacgct caagaaacac tagcttatct
    15001 tcatgaggaa ggtgtggatc tgaaagtgat ttctggagac aatcctttta ctgtctccaa
    15061 tatcgctaga cgtgcaggat taccgaatta tgatgcgtat gtcgatcttt ctcaaattac
    15121 tgaagaaatg gaagtgcgag aagctgctca tcgctatacg gtttttggac gagtgagtcc
    15181 gcagcaaaag aaattgctgg taaatgaatt gaaagaaagc ggcagaacgg ttgcaatgac
    15241 aggcgacggt gtgaatgatg ttttggcgtt aagagaagca gattgcagca ttgctatggc
    15301 agaaggtgat ggagcaacac gtcagattgc gaatctggta ttgttggact ctgattttac
    15361 tacattgcca gaagtcctgt ttgaaggacg ccgagtggtc aacaatgtga cgaaagtctc
    15421 aggcatcttt tttatcaaaa cgatttattc atttatttta tcgattatat gtgccgttac
    15481 agcaattgga tttccgttta tccctatcca aatcacgctg attgatttag cgattgaagg
    15541 gtatccttcc ttctttttat cttttgaaca agataaaaga aaaatcactt accgttattt
    15601 gcctacagca cttattaatg ctttaccaaa tgccttgctt gtagtagtga atatcattgc
    15661 ggtgtatcta cttggaaaaa cattcgcatg gggaaatgct gaaactacta ctttgatgta
    15721 ttacttgctg attgggatca gttgcatggc agttgtaaaa gcttgttatc ccttcaaccc
    15781 attgcgcatc ttcctagcag taacgacgat cgtagggata tatgtagctg caatgctgtt
    15841 ccatggaatt ttggaagtaa acttagggtt gggagagagc gtaggatact tctttgtatt
    15901 catgctcgta aatattgttc ttcgagtatt gtactggaag gctgatattc aaaatcagtt
    15961 actgaaaaga gtagcaaatt gatgcttaat gagatagaaa tgtgttatac ttgaaaaagc
    16021 aatgaaatat gatacaatca aacatgtata attgtgtcct ttaaaatcgc actgatttca
    16081 gttagtgcga ttttttaccg gttattctcc tgaaatatct tctgttaaac ccaaaagata
    16141 tcaatagagc agggggtgaa aaaatggctc aacggattcc tcaagaggtt atcgaaacag
    16201 tacgcagtca aacgaatatc gttgaggtga ttggacaata tgtccagttg aaaaagtctg
    16261 gtaaaaatta cttggggctg tgtccttttc atgaagaacg tacaccgtct ttttctgttg
    16321 cagaagacaa gcagattttt cattgctttg gttgtggtaa aggcggaaac gtgtttactt
    16381 ttcttcaaga actagaaggt ctaagttttc cagaagcagt ggtaaaagtg gcagacttcg
    16441 aacagatacc gttagaagat caatggcgtc agcgtactat agctgtccaa aatcctgaat
    16501 ctgctacggg gaaactgatt gctgctcatg aaaaagcagc ggaaatctat catcacatgc
    16561 tattgcatac aaaagctgga cagccagctt tagaatattt gcaaagtcgt gggttgtctt
    16621 tagaattaat tgaagaattc aacataggtt ttgcacctaa tgagcgttct tttcttcagc
    16681 aagtattcaa gaatgaatca atttcaaatg aattgttaga acgctctgga ttgtttgtca
    16741 cgcatgatga tggccgtatg tctgatcgtt tttatcaaag agtgatgttt ccacttcgta
    16801 attctcaagg ccagacgatc ggtttttcag gacgctggct tgcacctgaa aaagggcagg
    16861 aaaaagatca gcccaaatac ttgaatagtc cagaaacaga actgttcaat aaaagacaag
    16921 ttttatttaa tcttgataaa gcaagaagcg atatccggaa aaacggagaa attttgttgt
    16981 tcgaagggtt tatggatgtg attgctgcat ggcaggctgg tatcaaaact ggattagctt
    17041 ctatggggac aagtctaaca gctcaacaga tccaacaaat ggaaaagctg acgaaagaat
    17101 tggtcttttg ctatgacgga gacaacgcag gttttgaagc aaccaatcga gggattgaat
    17161 tgcttcggca aaacagtcga ttagcacttt ctgtcgtagt cgttccagaa aagcttgatc
    17221 ctgatgaata tctccgtaag tatggagaag catctttcca agaattagtg gctcatgggc
    17281 gcgaaacagt ctttactttt aaatcacgtt atttaaaaca agggaaaaat ttagataatg
    17341 aaaaagacaa gatcgaatac ctcgaagaat tactagttga acttagccga gttgtttcac
    17401 caatcgaaca agatatgtat ctatctcaac tgtcaacaga gtttcagatc ggcagagaga
    17461 cgatacaaaa acaactgcgc gatcttcgaa ggacaaatca gcaaaatcgt caagaacctc
    17521 aggaaagtca acacactcag gttacgagac aagaaactgc acggaaaaag agacctttga
    17581 cgcaagtaga aaaggcagag cagattctat tatatcgtgc atttaaagaa gtttctgtcc
    17641 gtaatatttt aaaagaacga gaatttcagt ttattcatga tgcatatgca gaagtttatt
    17701 ttttattcga tgcatatgtc tcacaacatt atgaattcaa ccttgcagag tttttagact
    17761 ttttgcaaga tgaaaattta aggcggttgg tggtggaaat cgaatattta ccggtggcag
    17821 aagaaagttc gccacgagaa atccaagact tgctagatgt gatcagcaaa tctggcttag
    17881 ctgatgaaat aacattaaaa aaacaaatgc agcaagaagc cagacgaact ggaaacaaac
    17941 agcttgagct tgaattgacg attgaaatca tcaatttaac gaaacagtta aaacaagcca
    18001 aatagctact caaggggggc cttcttttat ggcaaatgga acagataaaa aaacgtatga
    18061 agcagcagta gcagctttca tcaaagaaaa caaacccaaa cgacaagttg tatatgatga
    18121 tttatccaat cagcttgcta caccattcac tttgaatgca gatgaaatgg acaaattgat
    18181 ccaaaagatt gaagacgcag gtatcagtgt ggtggatgaa aatggtgatc catcaattca
    18241 cagcttgaag agtgctgaga aaaaagctga acaagcaaaa acagaagatc tttctgcgcc
    18301 aaccggtgta aaaatcaatg acccggttcg tatgtatctg aaagaaattg gccgtgtctc
    18361 ccttttaact gcggaagaag aagtagcgtt agcattgaaa atcgaagaag gcgaccaaga
    18421 agcgaaacag cgtctggcag aagcaaacct tcgtttagta gtaagtatcg ctaaacgtta
    18481 tgtaggacga ggcatgcagt tccttgactt gatccaagaa ggaaacatgg gactgatgaa
    18541 ggcagttgaa aaatttgact accgcaaagg atttaagttt tcaacctatg ctacatggtg
    18601 gattcgtcaa gccatcactc gagcaatcgc tgaccaagca cgtacgatcc gtattccagt
    18661 tcatatggtt gaaacaatta ataaattgat acgtatccaa cgtcaattgc tgcaagacct
    18721 tggaagagaa cctacgccag aagaaatcgg tgcggaaatg gatcttccaa cagaaaaagt
    18781 ccgagaaatc ttgaaaatcg cgcaagaacc tgtttccctt gaaacgccaa tcggtgaaga
    18841 agatgactct cacttaggtg atttcataga agaccaggaa gcaacaagtc cagcagaaca
    18901 cgcagcttat gagttactaa aagaacaatt ggaagatgta ctagatacat tgactgatcg
    18961 ggaagagaat gtccttcgct tacgttttgg tttagatgat ggccggacga gaacattgga
    19021 agaagtaggg aaagtatttg gtgttactcg ggaacgtatt cgtcagatcg aggctaaagc
    19081 attaagaaaa ttgcgccatc cttctcgttc aaaacaatta aaagatttct tagaataagc
    19141 gtggactatt gaccagaaga gaaatcagta aaactgtttg gtttatttta aaagaaaaaa
    19201 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
    19261 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
    19321 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
    19381 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
    19441 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
    19501 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
    19561 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
    19621 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
    19681 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
    19741 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
    19801 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
    19861 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
    19921 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
    19981 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
    20041 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
    20101 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
    20161 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
    20221 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
    20281 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
    20341 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
    20401 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
    20461 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
    20521 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
    20581 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
    20641 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
    20701 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
    20761 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
    20821 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
    20881 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
    20941 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
    21001 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
    21061 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
    21121 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
    21181 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
    21241 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
    21301 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
    21361 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
    21421 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
    21481 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
    21541 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
    21601 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
    21661 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
    21721 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
    21781 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
    21841 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
    21901 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
    21961 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
    22021 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
    22081 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
    22141 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
    22201 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
    22261 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
    22321 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
    22381 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
    22441 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
    22501 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
    22561 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
    22621 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
    22681 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
    22741 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
    22801 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
    22861 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
    22921 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
    22981 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
    23041 catggaatct gtcaggaatg ctatgaaaaa gaaa
//
LOCUS       DACDKJ010000042        23066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDKJ010000042 DACDKJ010000000
VERSION     DACDKJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.042"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IV328_001810"
     CDS             complement(109..564)
                     /locus_tag="IV328_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937947.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IV328_001811"
     CDS             complement(718..1926)
                     /locus_tag="IV328_001811"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8937948.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IV328_001812"
     CDS             complement(2038..2370)
                     /locus_tag="IV328_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8937949.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IV328_001813"
     CDS             2518..3393
                     /locus_tag="IV328_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8937950.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSGELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IV328_001814"
     CDS             complement(3475..4056)
                     /locus_tag="IV328_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8937951.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IV328_001815"
     CDS             complement(4062..5321)
                     /locus_tag="IV328_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8937952.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IV328_001816"
     CDS             complement(5323..6369)
                     /locus_tag="IV328_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937953.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IV328_001817"
     CDS             complement(6604..6879)
                     /locus_tag="IV328_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8937954.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IV328_001818"
     CDS             complement(7122..8432)
                     /locus_tag="IV328_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8937955.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IV328_001819"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IV328_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8937956.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IV328_001820"
     CDS             complement(9978..10658)
                     /locus_tag="IV328_001820"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8937957.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IV328_001821"
     CDS             complement(10707..11342)
                     /locus_tag="IV328_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8937958.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IV328_001822"
     CDS             complement(11408..12811)
                     /locus_tag="IV328_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8937959.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IV328_001823"
     CDS             complement(12798..13829)
                     /locus_tag="IV328_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937960.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IV328_001824"
     CDS             13860..14081
                     /locus_tag="IV328_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8937961.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IV328_001825"
     CDS             complement(14246..14983)
                     /locus_tag="IV328_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8937962.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IV328_001826"
     CDS             complement(15136..16476)
                     /locus_tag="IV328_001826"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8937963.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IV328_001827"
     CDS             complement(16713..18236)
                     /locus_tag="IV328_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8937964.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IV328_001828"
     CDS             complement(18614..19225)
                     /locus_tag="IV328_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8937965.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IV328_001829"
     CDS             complement(19555..20271)
                     /locus_tag="IV328_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8937966.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IV328_001830"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IV328_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8937967.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IV328_001831"
     CDS             complement(20830..21627)
                     /locus_tag="IV328_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8937968.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IV328_001832"
     CDS             complement(21597..21968)
                     /locus_tag="IV328_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8937969.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IV328_001833"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IV328_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8937970.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3911 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctggtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaaa
//
LOCUS       DACDKJ010000043        22807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDKJ010000043 DACDKJ010000000
VERSION     DACDKJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.043"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            92..502
                     /locus_tag="IV328_001834"
     CDS             92..502
                     /locus_tag="IV328_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8937971.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            495..1196
                     /locus_tag="IV328_001835"
     CDS             495..1196
                     /locus_tag="IV328_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8937972.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1196..2782
                     /locus_tag="IV328_001836"
     CDS             1196..2782
                     /locus_tag="IV328_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8937973.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2801..3022
                     /locus_tag="IV328_001837"
     CDS             2801..3022
                     /locus_tag="IV328_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8937974.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            3019..3780
                     /locus_tag="IV328_001838"
     CDS             3019..3780
                     /locus_tag="IV328_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8937975.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4160..4669
                     /locus_tag="IV328_001839"
     CDS             4160..4669
                     /locus_tag="IV328_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8937976.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4744..5283)
                     /locus_tag="IV328_001840"
     CDS             complement(4744..5283)
                     /locus_tag="IV328_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8937977.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5423..6034)
                     /gene="rpsD"
                     /locus_tag="IV328_001841"
     CDS             complement(5423..6034)
                     /gene="rpsD"
                     /locus_tag="IV328_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8937978.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6350..7147
                     /locus_tag="IV328_001842"
     CDS             6350..7147
                     /locus_tag="IV328_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8937979.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            7175..7810
                     /locus_tag="IV328_001843"
     CDS             7175..7810
                     /locus_tag="IV328_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8937980.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7830..8603
                     /locus_tag="IV328_001844"
     CDS             7830..8603
                     /locus_tag="IV328_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8937981.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            9011..9808
                     /locus_tag="IV328_001845"
     CDS             9011..9808
                     /locus_tag="IV328_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8937982.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9786..10199
                     /locus_tag="IV328_001846"
     CDS             9786..10199
                     /locus_tag="IV328_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937983.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10183..12828
                     /gene="mutS"
                     /locus_tag="IV328_001847"
     CDS             10183..12828
                     /gene="mutS"
                     /locus_tag="IV328_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8937984.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12846..14954
                     /gene="mutL"
                     /locus_tag="IV328_001848"
     CDS             12846..14954
                     /gene="mutL"
                     /locus_tag="IV328_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8937985.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14976..15536
                     /locus_tag="IV328_001849"
     CDS             14976..15536
                     /locus_tag="IV328_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8937986.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15621..17609)
                     /gene="treP"
                     /locus_tag="IV328_001850"
     CDS             complement(15621..17609)
                     /gene="treP"
                     /locus_tag="IV328_001850"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8937987.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17832..18545
                     /gene="treR"
                     /locus_tag="IV328_001851"
     CDS             17832..18545
                     /gene="treR"
                     /locus_tag="IV328_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8937988.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18622..19068
                     /gene="msrB"
                     /locus_tag="IV328_001852"
     CDS             18622..19068
                     /gene="msrB"
                     /locus_tag="IV328_001852"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8937989.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19238..19840
                     /gene="ruvA"
                     /locus_tag="IV328_001853"
     CDS             19238..19840
                     /gene="ruvA"
                     /locus_tag="IV328_001853"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8937990.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19853..20854
                     /gene="ruvB"
                     /locus_tag="IV328_001854"
     CDS             19853..20854
                     /gene="ruvB"
                     /locus_tag="IV328_001854"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8937991.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20883..21461
                     /locus_tag="IV328_001855"
     CDS             20883..21461
                     /locus_tag="IV328_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8937992.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21513..21995
                     /locus_tag="IV328_001856"
     CDS             21513..21995
                     /locus_tag="IV328_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8937993.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22112..22486
                     /locus_tag="IV328_001857"
     CDS             22112..22486
                     /locus_tag="IV328_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8937994.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7423 a         4075 c         4931 g         6378 t
ORIGIN      
        1 tcaaaaattg tatcttagaa acagcatgtt ttgaaacttt cagaaaaggt ttgttagtaa
       61 aatgaaagct atacgtaagg agtgttgttt gatgaaattt tttcaagagt ttttcattat
      121 tattttcttt tctgtattag gagaaggcat acggattttt gctcatttac ctattcctgg
      181 aagtatctta ggtattcttt ttttattttt agcatttgaa atgcatcttg ttgatcctga
      241 aaaaatcgga gcaactggtg attttttatt gaataatcta gccattttat ttgttcctgc
      301 tggtgtaggt ttattggaat attttgatga tattgcttcg atatggcctg ttttactggg
      361 agcagtcgtc gtgtgttcca tcgtcacgat ggttgcagcg ggaaaaacgg cagaaggtgt
      421 agaagctttg ctgggctatg tccgtaagaa aaaagcagaa agggtatctg taaacgaaat
      481 ggaggagaat caaattgact gagttttttg tgaatccatt atttggctta agtttgactt
      541 taggagtgta cgtattatct gacaaatttt tgcggaaata taaactgcct gttttgaatc
      601 ctctcgtatt atcgattgtt gttattatct tatttttgca tttttctggg atcagctata
      661 aagactataa tatcgggggc aattttttga ctatgctgat tactcctgcg acagtggcac
      721 ttgccatccc tctttataaa aacttccgtt tactgaaaga gaatttttat cctgtaatgg
      781 ctgcgatttt agcaggaata gtcgcaaatt gcttggttag tgtagggttc ggttacttct
      841 tctctcttca taaagagatg gtgatctcat tactacctaa atctgtaacg acggctattt
      901 ctgtcgattt atctcatacg atgggcgggg tgaatgccgt gacacttgct attgttgtct
      961 ctacaggtat ttttggttcg ctgatcgcca ctcatatttt ccgcctattc aagatcgaaa
     1021 gtccagttgc tcgcggtgtg gcgttaggaa gtacttctca tgctattggt acagcaaaag
     1081 caatcgagat tggtgaaata gaaggaatca tttccggact tgcgatatgc gtcaacggaa
     1141 tattgaccgt tttattgcta ccgttattct ttcagccttt tgctggcttg ttttgatgcg
     1201 aaaaccacta ttttatgcta caataaccaa aacgttcgag cataaaaaag gagggaaaga
     1261 cgtgtatttg tcttccgagt atcttaaaca atttgataaa gatctatatt ataagattct
     1321 cctcctagaa agttttgagg atcaagcttg gcataccgct gcacaattag cgcaagttgt
     1381 tcagctggat gcccgcagcg tgtcgaaata cctgaacgag ctgtctaaaa actatcagca
     1441 gtttagcggt aagacacacc ctttgtttac aaaaaatcac cgttctggtt ataactttta
     1501 tgatacgctc gattcaattg aacatgagcg ttttttgatc tatctcgtgc aaagtacgct
     1561 gaaattccag ttgctgcacg acattttttt tgaagaattc catacaatgt accaatttgc
     1621 gcaaaagcat tacattagtg aatctactgc tcatcgaaaa atcaatgaat ggaaacagca
     1681 actgcaaact tacggaattc ggctgcaacg aggtacatat atcgctcaag gcgaagaaga
     1741 aatcattcga ctttacttgc atatgacatt ctggcagttg tttcgaggga aaatctggcc
     1801 atttgaaacg atttctcaaa tggacgtaaa gaatatggca gaacatatca tggctttttt
     1861 taatgttcgt ctgaacgaga tcaaaaaaag acgattggaa tatatgctag gtgccttttt
     1921 tttaagaaaa tcacagaagc attatgttgt attgaatgag aaaaaaagac ggctgatttc
     1981 tgacaatctg ttgttccagc gattttgcca ggtcatggag ccggtatttc ctaattattt
     2041 tcaagtagaa gacgagttag gcgcattatt tcttgtactg atgactcgtg aagaatatta
     2101 tagtgatcct aagatacgga agaaaatttt tgattttcat caacaagcaa aaacaccgcc
     2161 ctttacagcc ctttcagaag caaaagccgc tctttctctt tatcaggaag agcaggggct
     2221 gccagcagaa aatctaacct tcgaggcaga aaactattta ttcagcagtc attttttcgc
     2281 ctatcttttt cctaatgcga aagaaacgat tgatggcaat agtagtgatt ttattaatca
     2341 tttagttatt gaaaacaagg aattgaaaca atggctcgta catttttttg aaagtagaca
     2401 caaacatccc aatcatctgg catttaaaaa tcatgcgttt ttgatggggc ggtatctgac
     2461 tgtgtttaaa acattaggag cctttactcc acagcttcca aagatcacga tccttttaat
     2521 gacagatttc ccattatttg aagagcaact gttagaggag ggattacgga catttttccg
     2581 taatgagtat cagctgattt ttctgccaac agattatcgg gggagagaag ttgatcttct
     2641 gatttcgaca agtaaagtcc accgcaaacc atgggcggat cttgactatt ttattgtgac
     2701 tgaagaacta aaattaattg attacatcca actatctcaa aaatttgaaa tgattcaaaa
     2761 acaaaaacta ttaaaacaat aacaaagaag gagaaaaaag atgacatttg aagaaatttt
     2821 accagaattg aaaaaaggtg caaagattat tcgtaaagga tggagtggat ttgaacttta
     2881 tgttgttctc gtatccgatg atgaatttga tggctgtccg gtgacgccgt acttattgat
     2941 caaaacaagt gacgaaggtt tttccagctt cgctcctaca gtttgtgata ttttagcaga
     3001 tgattgggcg atcgtagaat gaaaaaccag tatccagatc tagctaaaca ggttgtattt
     3061 atcacaggag ctgcttctgg aattggtgct gcgcaagccc gagcgtttct tgaacaagat
     3121 gcttttgtat ttggattaga tcaaaagttc ggtaaaatgg aagaaatttc tttgcgattt
     3181 cctgaaactt ttgcctacgc actaggtgat gttcgacaaa tgaaagaact ccaacaagcg
     3241 gtatcgaaat gccaaaatca ttttggtgaa gttacgattt tactgaatac agcggggata
     3301 ttggatgcct acaaaccctt gatggaaact gacgaatcgc tgtgggatct gatttatgag
     3361 acgaatgtga aaagcatgta tcagctgaca aaaatcatcc ttcctgatat gatccgccaa
     3421 aaaagcggaa cgatcatcaa tatggcttcg attgctggct tgattgctgg tggaggtggg
     3481 atcgcttata caagcgcgaa acatgcgatt gtcggattta ccaaacaact cgctctggat
     3541 tacgcaggac agggtatccg agtaaaagga attgctccag gagccatcca aacaccaatg
     3601 aatgcggctg attttgaagg gaaaggtgag atggcagagt gggtggcaaa tgaaacacca
     3661 gtcaagcgat gggcacagcc ggaagaagta gctgagctga cgttatttct tgcctctcct
     3721 caagcaagct acatacaagg aacgatcatc ccaatcgacg gcggatggtt gttaaaataa
     3781 ccttgtgttt ttttcaagct tctgtatgat aaaactaaag agagaaacaa cggttgatga
     3841 taaaaaaagt cgatgactgt tcccgacatt cgatgccgaa cagccagttc aaaaaattct
     3901 ttttaataat cgttaatatt cgaaactaag cggcttttca cgacctcttg atataaacga
     3961 tattcaaaag cttgcaactg cggtaacaag tccagccaaa tgaaaaatat aaggagctat
     4021 ttttatttga ccattcttat tacttgttgc agggcagctt tttcactacc ttctgcgaca
     4081 cttcagttgg aattttcttc ccatcctttg gtcgcggtca ttaccaagat aaaattatct
     4141 tgcaaaggag aaatagaaca tggaaaaaaa taatacccgt tcagccaaac gctggacaac
     4201 gacagatgtg actcggatgg cgctagtcac tggtttatat gtggcagtga cgctggtttt
     4261 gtctgtcttt agctttggag tgattcagat tcgtctctcg gagatgttca actatctgtc
     4321 cttgtataac aaaagataca tcgtcgcggt aacagcggga gtagctttgg ctaatattgc
     4381 ttctcctctt ggactgatag atgttgcagt aggaagcgtc agcacgctgc ttgtattgct
     4441 tatcaactat aaaatcacta gtcgcatcaa aaacatgaaa atcaaaatgg ccgtgacagc
     4501 atgcgtattt gctttttcca tgtttacagt agctggtcag ctaacgattt tatatcaact
     4561 accttttttc ttgaattggt gggtgattgc tttaggagaa ttggcatcaa tgattgtcgg
     4621 tgggataatt atttattgga tcggtaaaaa agtggatttg tcaaaataaa gatctgttta
     4681 atttaaaata gggcagcatg gaagagactg aatgttgagt cttcttccat gctgcttttt
     4741 tgattaattt gtatgaagaa atcgatttag ccgttttttc atcaagatac cgatataagc
     4801 tgctccagtt gccttgattg cttccggcag taaaaacggc agtagtccac tggcaaatgc
     4861 agttgtaaaa gtaagatcac cacttatttt cagccagatc gttccgcaga tcaatgcagc
     4921 aagagagccc attagattgg caaagattgc atataagtaa tgagcatccg ttctttcgat
     4981 catccaacca gttatccacg tttgtaagag aaatccaatc aaatatccgc cagttggccc
     5041 aaataaaaca gctatacctc cagtcatccc tgaaaataca ggaagaccaa tcaagccaag
     5101 taaaagataa actccaacag atatcaatcc tagttttctt ccgagaaaag ttgctgttaa
     5161 tccaatagca aatgtttgcc ctgtcaaagg aaccaaacca aaaggaatgg tcatctgaga
     5221 aaggacagaa ataatcgcgg caaattccgc agcaagtatc aattctttca atgttcggtt
     5281 cataacgccc tcttttcgtt aacttaatat ttttatttgg ttaacgaaat tgtaaaacaa
     5341 agtgattact ttgtaaatag aaaatataaa aaaagaccag ctatccaata gatagcggtc
     5401 tttttcaaga aagcttttga aattataagc tttggttgta gtattcaacg acaagagctt
     5461 cgtcgatttc tggatatagt tcgtcacgtt ctggtaaacg agtcaatgaa ccttccaatt
     5521 tttcagcgtc aaagcttacg aatgctgggc gtccaacagt tgcttctaca gcttctttta
     5581 ctgttacgat gttttgtgat ttttcacgaa ctgagatcac ttgacccact tctacgtggt
     5641 atgaagggat atcaacgcgt ttgccatcaa ctaaaatgtg accatggtta actaattgac
     5701 gtgcttgacg acgagtcgtt gcaagtccta aacggtaaac aacattgtct aaacgttgtt
     5761 ctagtaagat catgaagtta acaccatgtt taccttcttt gattttgcta gcttttacga
     5821 acaagttacg gaattgacgt tcgttcattc cgtacatgtg acgaagtttt tgtttttcag
     5881 ttaattgcat gccgtattct gattgtttgc cacggctatt aggtccatgt tgacctggtg
     5941 cgtaagggcg acgtgctaat tcttttcctg tgcctgacaa tgagatgcct aagcgacgag
     6001 aaattttcca tgatggtcct gtataacgag acattaaaat ttcctccaat gaatataagt
     6061 ttttggagta aaataatccg ttgaaaaatt cataatcata tagtttgttc ttcaatcttc
     6121 acctttgcag ccgcggttac acaatcgaac ccgagacggg gcaacaaact agggatgagt
     6181 tattattttt ctgctgcatt attttacaca aattacagta tatgatagat catccacgaa
     6241 tgtcaagata attagtacat aaaaacgctt tacaaaccta ggctgtcttt ataaaatgag
     6301 agggtatgtg caaatggaaa aaagcgaagg aagtttaagg agaaaatcaa tgaatccggt
     6361 atcaccatta tttgagaaaa tcgatgcttc aattgaaaag aaggcgaatc tttttgaaaa
     6421 cagcttttca cgttacgctg ttcgagcggt tctcgcctgt ttgtttttga ctttaggaac
     6481 ggctgtggca tttgcgatcg cgatgaaggg ggaagatatc gcgcatggtc taggaaagat
     6541 tttatacgca tttatgttca gctggggtct ggtcatgatt ctatacatga acgccgaatt
     6601 aggaacatcg aatatgcttt atatgaccgt gggtgttttt cggcagagac ttgctttaag
     6661 taaggcagcc aagattctat ttgcctgtat ttttttcaat atgattggcg gtatattatg
     6721 tggatttcta atttctatga ctacgccatt tcaagattta acgttggaca actttatgtt
     6781 agaatcaata gcagtaaaac tagcaaaacc ttcagcaacg attttggtgg aagggatgtt
     6841 tgcgaatatc gttgtcaata ccgctgtact gatcagcatg cggatgaaag aggatgtcgg
     6901 aaaagtgatc acagtcgtat tcatcatttt catttttgct tttttaggat atgaacacgt
     6961 gatagcgaat tttcctgcct ttacgcttac ttactttgct tctcacggac agatggatgg
     7021 aatgacagtc agcaatgtgt tacataattt gttttttgct ttggcaggaa attatattgg
     7081 tggcggttta gtaatgggac tgggatatgc ttggctgaat cagtcaaaat catcctatgt
     7141 ggactaaaaa gtcgttcatt aaaggggaag tgccatggaa gttttagttt ttccaaaaaa
     7201 tgaggaaaaa gcgttacaaa tggaaaagta catgaaaaat cttttttcct ttgctggagt
     7261 acctaagccg gaacgcgctc ttttacaaca accgttactg gatgagagca aaaatttatc
     7321 tatttctgaa gttttagctt ttgttcatta ttattatgaa caaatcgaaa gagagtatca
     7381 gtatgtagcg atcgatttag tccagaaaaa catcaaacgg cttacatttg aagacttagt
     7441 cgtattgttg ccactagttc agaaaaaaga atggtgggac agcatcgact cttggagaaa
     7501 agtatacagc acatggtgta aagcctaccc agagcagcgt gaagaagtct ttcaattgtt
     7561 tgaaaaaaag gaagattttt ggctacgtcg aatagcaatc acgttgcaat taggttataa
     7621 acagcaaaca gacacaggac tattgatccg agcaatagac gaagatcgtc aaacaaagga
     7681 attctttatc caaaaagcaa ttggttgggc attacgcgat tatagtaaga ccgatgccga
     7741 ttgggtaaaa agacagctga caacagattt gagtaagtta gctgttcgag aggcaagtaa
     7801 gtatttataa gaaagcgaga cgacaagaga tgagacgcaa attatttgct tttgatattg
     7861 atggaacgtt gttagattca aacaaacaag ctttagatag cacaagagaa gcattggaaa
     7921 aattacgcaa agccggtcat ctggtcacga ttgctacagg gcggagccga tttcatgcgc
     7981 aagagatcat tcgcgatctt gattttacga actacatttt atgtaatgga gcggccggtt
     8041 ttttagacca tgaacaagta tataagaatt tactagatcg cgaccagttg gatgcgttcg
     8101 ttcaagaagc gcatcatgag cagatcgata cagcttttgt cagcttagac aatatcaagc
     8161 gttcttcttc caatgatgta acggtgatgg aagaagcgat gcattctttc ggtgctgtct
     8221 tgcctgaatt agatcagttt tttgcagagg aacaagaaat ataccaagcg ttagcatttt
     8281 tcgatcaaac atatgatggg aagttctctt catatgataa aattcgattt gtccgctggc
     8341 atgagaacag tgtagatgtc gtacctaaaa atggttctaa agcagcgaca attctgcact
     8401 tagcagaaca agtaggcatc cctcatgaag acatcatcag ctttggcgat ggacaaaacg
     8461 accgtgaaat gttgcgcatg tcaggaattg ggatagctat ggggaatgct gtgccagaag
     8521 tacaagcaga agctaagatg gtcactgata caaacgatca ggatggaatt tggaaggcat
     8581 tgaaagagtt aagcgtgatt taaaagaggt tgtgaaaaag ctgttctgca tcaagtaata
     8641 agcccagcca aacaaaaata gctcctccta tttttcgttt ttctgggctt attaccgcag
     8701 atgcaagctt tcgaacaccg tttaaggaag aggtcgtgac agaagtggtc agtttcaaga
     8761 aataagaagg gatttacgaa aattgcttct caaatttttg taaaattacg gcttatttct
     8821 gaagaagtag atgaaacaac gtttattcgg gaaatgggac tgtgacaaga tgtttgtcat
     8881 agtcccattt ttttatttta tttttcaaaa ctaagaaata ccaggaaaga gttccttttg
     8941 tttgaccttt tctttcttat tgaaaacaac tataatatag agtgatgtta aaagagaaga
     9001 ggaatttagt gtgcgggtat tgtttattgg tgatgtagta ggttcaatgg gacgagagat
     9061 gattactgaa tatcttccta gattgaagaa gaagtaccgt ccgcaagtaa cgattgtaaa
     9121 tggtgagaat gccgcttctg gacgtggaat cacagaaaaa atctataaaa aatttttgca
     9181 agacggcgta gatgtcgtga cgatggggaa tcatacgtgg gacaaccgcg gaatctttga
     9241 gtttgtaaat gaagctaaaa agatggttcg tccggcaaat tttccggaag ggacgcctgg
     9301 acaagggatg gtctttgtca aagtgaacca agtcgaactg gcagtgatca acatgcaggc
     9361 tcgttctttt atggtggact tggatgatcc ttttcgtaaa atgaatgagc tggtagaaga
     9421 agccagaaaa cgtacgccga tcatttttgt agattttcat ggagaaacaa caagtgaaaa
     9481 acaagcaatg ggctggtttt tagatgggaa agtttcagca gtggtcggta cacatacaca
     9541 cgttcagaca aatgatgcgc gaattttgcc aagaggaaca gcgtatctga cagacgttgg
     9601 gatgacaggt ccctatgatg gtattttggg tatgcgcaga gaacctgtaa tcgaaaagtt
     9661 tctgactgca ttaccaaaac gttttgaagt tgtggaacaa ggccgatcga ttttaagcgg
     9721 ctgtatcttg gaattggatg atacgacagg tcatgcaaaa gaaatccagc tgattcagat
     9781 caacgatgat cgaccgttca tggagtagaa agagaggcag ttttttgcag caggagccgg
     9841 aaattcaaaa agaagtaagg aaactgacaa aacttttaag agagaatgaa acgatcattc
     9901 gctacaaaga acttgaagaa aagatccagc aaaaccagta tttagctgag ttgagagaaa
     9961 aaatcaaaca agcgcaaaaa gatgctgttc attttgccca ctatgacaaa ccagcagcag
    10021 aaaaagaagc aatcaaacaa gcggatcaat tcatgcaaga atttgatcag catccgcttg
    10081 ttgtagctta tagaaaacag ctgttggaag cagatgatct gcttcatcat ttaacaacta
    10141 tgatacaaga agaaatcaat ggacaaatag aggaggaaaa acatgcctca aaaaactaaa
    10201 aatactccta tgatggaaca atatctaagc atcaaggccc aataccaaga tgcttttttg
    10261 ttctatcgtt taggagactt ttatgaacta ttttacgaag atgcgatcaa agcgtcacag
    10321 cttttggaat taacgttgac gagcagaaat cgaaatgcag aagaacctat tcctatgtgc
    10381 ggtgtacctc accatgcagc acaaggatat atcgatgcct tgattgaaaa aggctataaa
    10441 gtagcgattt gcgagcaggt agaagatcca aagacgacta aagggatggt caagcgcgaa
    10501 gtgatccagc tagtcactcc tggtacagtc atggacagca aaggattgtc ggcaaaagac
    10561 aacaattttt taacagctgt agttagtcaa ggaaatgaat ttggctttgc ttatgccgat
    10621 ttaagtacgg gagaattgaa gacggctcgg ttgcatgatg aagaagctgt tttaaatgaa
    10681 gcctctgcat tgcagacaaa agaactcgta ttaggcagtg agatcactga tacccttcgt
    10741 gaacagctaa gcgggcgttt agggctagtc ttttctcaac aaaatgagat ggaagaaaac
    10801 gcggaattta gctttttgac aagcgaacta gtcgatccgc tggaaaaaga agcaaccggc
    10861 aaactattga cttatttatc ggtgacacaa aagcggagtt tagctcatat ccaaaaagca
    10921 gtagaatacc aaccagatca ttttttgaaa atggattatt attcaaaatt caacctagaa
    10981 ttaagccaat ctattcggac tgggaaaaag cacggcactc ttctatggtt gttagatgaa
    11041 acaaaaacag caatgggtgg ccgtttacta aaacaatggc tagaccgtcc gcttatccag
    11101 gcaaaacaaa ttaaagcaag acaagaaatg gtcgcttctt tgctagacag ctattttgaa
    11161 cgtatcgatt tgcagtcagc attaacgaaa gtctatgatc tggaacgcct agcaggtcga
    11221 gtagctttcg gtaatgtcaa tgggcgagac ttgatccagc tgaaaacttc tttagaacaa
    11281 gtgccaatga tcagagggtt gattgaagga atcgatcaag gtcaatggca aagtctgctt
    11341 tcagaaatgc agccgatgga ccatctcgtc caattgatcg accaagctat ccaagatgat
    11401 ccgccgttac aaataacgga aggaaacatc atcaaagatg gattcaatga gcagctcgat
    11461 atgtaccgtt ctgctatgag aaacggaaag aaatggttgg cagaacttga agcaaaagag
    11521 cgtcaagaaa caggaatcaa gaacttaaaa atcggattta accgcgtttt cggttattat
    11581 atagaaatta caaaagcaaa tcttggaaat gcagaactgg agaagtatga acgaaaacaa
    11641 actttagcaa atgcagagcg tttcatcaca ccagaactaa aagaattgga gacacaaatc
    11701 ctcgaagcag aagaaaaatc tgttgattta gaatatcagt tgtttttagc tgtgagagaa
    11761 gaagtcaaaa aggctatcca gccgctacaa gtacttgcaa aagcaatcag tgcagtagat
    11821 gtgctacaaa gttttgcaac gatcagcgaa cgctatcagt atgttcggcc agaacttgtc
    11881 agtgacaagc atcagcttct gatcaaagac ggacgacatc ctgtagtaga aaaagtatta
    11941 ggacatcagg aatatatccc taacagcgta gaaatggcag aagacgaaat gatcttgctg
    12001 attacaggac cgaatatgtc tggtaaaagt acgtatatgc gacagttggc attaactgtt
    12061 ctgatggcgc aaatgggctg tttcgttccg gcaaaacaag cagttttacc tatttttgat
    12121 cggattttta cccgaattgg tgcaagtgac gacttaattg ctggacaaag tacttttatg
    12181 gtagagatga tggaagccaa tcaagccctg cgttatgcta cgccaaacag tttgattttg
    12241 tttgacgaac tagggcgagg aacagcaacc tatgacggaa tggcgttggc ccaagcaatc
    12301 atcgaatata tccatcgtca tgtacaagca aaaacactct tttctactca ttatcatgaa
    12361 ctgacggtac ttgagaaaga acttccgcag cttaaaaatg tccatgttgg agccgttgaa
    12421 aaagacggag aagttgtctt cttgcataaa atgatggatg gacctgcgga taaaagctac
    12481 ggcatacatg tggcaaagat cgcaggattg ccttccaatc tactagaacg agcagcagat
    12541 atcctgcata cgctagaaaa tggcgataac ggacaccata ctgttccagc tgaagtagaa
    12601 gagaaaacta aaacagccaa acaggagcaa ataaaaccac ctgcagaaaa agtaaatcaa
    12661 gaccatatac aagaccgtgt aaaagaagaa aaccaacagc tttctttatt cagtgaatta
    12721 tccgaaaatg aaacagaagt cattgaatca ctgaagcaaa taaatttact agaaatgacg
    12781 ccaatggatg cactaaacac attatatgaa ttacaaaaac aactgtagga agaaggcgga
    12841 tatcgatggc caaaatccaa gaattatctg aacgtttagc taatcagatc gctgcgggag
    12901 aagtagtgga gcgaccagct tccgttgtga aagaattagt ggaaaatgca attgacgccg
    12961 gcagtactca gattgatatc ttagttgaag aagcgggact gcggacgatc caagtgattg
    13021 ataacggaga aggcattttg gcagacgacg tagagaatgc cttcaagcgg catgcgacaa
    13081 gtaagatcca taatcgcgac gatctttttc gcatacgaac gctgggcttt cgaggggaag
    13141 ctttgccaag tattgcctct gtttctgaaa tgactctgga aacggcgaat caggaagaaa
    13201 aacaaggcac ctatctgtcg ctgaaaggcg gagaagttat tgagcatcga ccagcaccac
    13261 tgcgtcaagg aacgaagatt accgtcagca atctgttttt caatacgcca gcccgcttga
    13321 aatacgtgaa aacactgcac acagaattag cgaacatcgg ggacatcgtc aatcgactgg
    13381 cattgagtca tccatcgatt gcttttcgtt tagtacatga tggaaacaaa atgctggcaa
    13441 ctgctggaaa tggcgatctg aaacaaacaa ttgcgggaat ctacggatta tctaccgcaa
    13501 aaaaaatgct gaaaatcgaa ggaaaagatc tagattttga gttatttggc tatgtttcat
    13561 tgcctgaggt gactcgtgcc agtaggaatt acttatcgac gatcatcaat ggacgtttta
    13621 tcaagaactt ttccttgaat aaagcaatcg ttgcaggtta tgggtcaaaa ttaatggtcg
    13681 gtcgtttccc tattgctgta ttggaaatca aaatggaccc gcttttagta gatgtaaatg
    13741 ttcatccgac gaagcaggaa gtccggctgt cgaaggaaaa agaactaaca gagctaatta
    13801 gtaacgccat tcaaaaagca ttgtctcaag aaaatctgat tccaagtgca gcggataatc
    13861 tacggtttaa gaaaaaagta cctgatcagc ctaaaacaga acaaatcgaa atcgatttgt
    13921 cctacgcttt tgaagaagaa aaacctaaaa agccaagtag tttgaatttt gatcgagaga
    13981 caggtacctt tttcgtagaa gaactaaaga acacatttgt tgacagaatt gttgataagt
    14041 ctgtggaaaa tgttgaaaac tattctggtt tttctgaaga aaacaccact tatccagaaa
    14101 aaacagcgaa aacggtggat aagtttggtg aaaatgttga taactttgta gaaaaaacag
    14161 tggaaaaatc tgaaaagaaa gaatttccca ttgtggataa catggagaaa acaaatgaag
    14221 aacaagcaga taaatccgta gaagaggatg aaaagattta cgaggaagaa ttgacactcc
    14281 atcctgaatt tgatttcact caaggaaacg acaaaacatt agaacaagcc atcaagaaat
    14341 tagaaacgga aaaaccagta caacgttttc ctcagttgga atattttgga cagatgcacg
    14401 gaacgtatct ttttgcccaa agtagcgatg ggttgtacat cattgatcag catgctgccc
    14461 aagagcgcat taagtatgaa tatttccggg aaaaaattgg ggaagtatct gatgatctgc
    14521 aagaattgct tgttccattt gtgttagact atccaaacag tgatgctttg aaattaaaag
    14581 agcaaaaaca cttgctagaa gatgcaggga tttatttaga agaatttggt caaaacagct
    14641 ttattgtacg ggcccatcct acttggtatc ccaatggaga agaagaatcg attattcggg
    14701 agatgatcga tatgctgctg acaactggaa gtgtgagtgt aaaaaaattc cgtgaagcaa
    14761 cagcaatcat gatgagttgc aaacgatcga ttaaagccaa ccattatttg aatgaagcac
    14821 aagcacgtgt gctgctgaag gatctggcac gatgcgaaaa tcctttcaat tgtccccatg
    14881 gccgtccagt attgattcac tttaccaatt cggatatgga aaggatgttc aaacgaatcc
    14941 aagacccgca ttaagtcaaa gagaagaggg aaaacatgaa gattgtatta gcatcccaat
    15001 caccaagaag aaaagagtta ttggctcata tcgtttcaga attcgagatt gaacctgctg
    15061 atatcgatga aacgccattt ccagacgaac agcccgtcga ttatgttcgt agaatggctg
    15121 aagaaaaagc acgaactgtc tgggaacaag atgaaaaaga agaccattta gtgattgcca
    15181 gtgacacgac tgttgttctc aatcaagaga tcatgggtaa accagaagat ctggctgagg
    15241 ctgaattcat gctgaaaaaa ctaagcgggg aaacacatac tgtatataca gctgttgttt
    15301 taaaaacaaa ggaacgagaa gaacggatac tagcagaagc acatgtcact ttttatcctt
    15361 tgacagacga agagatcaaa cgctacttgg agacggggga ttatgcggat aaggcagggg
    15421 catacggcat ccaaaatgct gcgagtgtat tcgtcaaaga aatttctgga gattattaca
    15481 gtatcgtagg atttccgatt ggtgctgtca atcaggcact gaaaacattc atttaaacaa
    15541 aaaagcggac tatctgattt tcgattgaaa atccatgata gcccgttttt tttattgaaa
    15601 agattactta tctactctga ctaagatttt acttcagctt taccgactgc ttcgcccggg
    15661 cgaacaagtc ctgattggtc aagagaaagt cctgcaactt tttcattatt ggtcaaagca
    15721 acaataatgt cagttttctt gccagcttgt tctatttctg gcaaatccat ttcagcaatc
    15781 agggtctctg gtgtgacaag gtatccttct ttcacgaata cattgaaagg aacacccttt
    15841 agttcaacag tatctaatcc catgtgaaca agtacttcca gcccctcatt agaaagaata
    15901 ccaatggcat gttttgtttc aaagacactc gtcactttac cgtttactgg tgcatagact
    15961 ttgccgtttg acggttcgac agcataaccg tcacccatca ttttttgtga gaaaactggg
    16021 tcatttactt cagttatttc cttgatggtc cctgcagcga ctgcgaataa tgtttctttt
    16081 gttgcaactg ccgtaccagt tgctgaaaca ggttcgaaag actgtggcgt tgctgttgcg
    16141 ccatttgctg tttgaagttg tacatcagca gcattttcag gaacagcagg atcaatctta
    16201 ttcaaaattc cctgacgtct aaagatgatc gtcaagccaa atggaatgat gacagcaatg
    16261 atcattgcga tgatgaaagg tacccatgtt tctgcttgga tagctagtat tcctggcaaa
    16321 ccgcctactc cgatcgcgtt agctcgaaca cccatcaagt tagcaaacat gccagctaat
    16381 ccagagccga ccattgcagc gacgaaagga tagacgtatt tcaaattgat tccgaacata
    16441 gcaggttcag ttacgcctaa gtaacaagaa atcattgctg gaatcgagat ttgttcttct
    16501 tttttattcc cacgatgtaa gaagatgatg gctaagactg cagagccttg ggcgatattt
    16561 gataaagcaa tcatcggcca taaattcgtt gatccgaaat ctgcaatcag ctgcatatcg
    16621 atggcattgg tcatatggtg gagaccagta atcactaaag gagcatacag gaatccgaat
    16681 acggcactaa acaaccagct gatagaagac gtcaatccag tatttacgat atttgagatc
    16741 cagcttccga ctgtccagcc gataggacct aaaataacgt gtgcagctaa aacagttggt
    16801 aacaatgaaa acaatggaac aaaaatcatc gagatcgact gagggatata tttgcggaag
    16861 aagatttcta gatacgctaa cataaaccct gccagcatgg ctggaataac ttgcgcttga
    16921 taaccaatca tctggacttg agcaaaacca aaatcccaaa atggaatatc tgctgcggct
    16981 gtggaagcta cactataagc atttaataat tgcggagaaa ctaacgtaat tcctaaaacg
    17041 attcctaata tttgtgtagt acccatcttt tttgcaatac tccaagtgat acctactgga
    17101 agaaaatgga aaattgcttc acccggtaac cacaaaaagg cattgacacc attccaaaac
    17161 ttcgagtgtt caacgattgt tccaccaaga ctttcaaact ggataccttc aagtacattt
    17221 ctgaatccaa ggatcaatcc accgacaatg attgcaggaa tcagtggtgt aaagatttct
    17281 gctaaaacgg caatagcacg ttgaacggga tgaaggtttt gttttgcagc tgcttttcct
    17341 tgttctttgg aaaccccttc aactccagat actgcaacga agtcgttata aaaagtagaa
    17401 acatcgtttc ctatgatgac ttggaactgt cctgcattcg tgaacatccc ttttacgcta
    17461 ggtatatctt cgatcgcttt ttcgtccgct tttcctggat cattcaggac aaaacgcata
    17521 cgagtcgcac aatgactaac tgcagcaata ttttcttttc cgccgatatc atgtaacagc
    17581 ttctcggcat ctgcttgata ttttgccata tcaataaacc cctttctctt aacttgtcta
    17641 tgcaagttgt ttcttcattt actatacttt ttttcggaga gaattgcaag cgttttatta
    17701 aaaagtacga gagaaaatga tatcgtttta aaaagcttaa aataagcttt tcttaaaaat
    17761 aggaacattg aaatacaaaa agagctgtag tatagtgaag aaatgatgga agaacgagtg
    17821 agggaaaaaa catgaataag ttcaatgaaa tttttttaga tttggaacag aaaattttag
    17881 cgaaagaata tcctccgcat tcgcttttac ccagtgagaa ccaattgatc aaaatttatg
    17941 gtgtttcacg agaaacaatt agaaaagcgc tgaatctttt gactacggaa ggttatattc
    18001 aaaaaaaaca aggaaaaggt tcgatcgtac tggatcggaa tcgcttcgac tttcctattt
    18061 ctggattgac tagctataaa gagctacagg agacccaacg tattccaagt gaaacgatca
    18121 tccatacgtt agaagaaaca gaagtgacaa aagcattgaa cgagatcact ggctgggaga
    18181 tcggtgcgcc tgtttggcat ttgatacgcg aaagaaaaat caatggcgaa gtagttatat
    18241 tagacaccga ttatctatta aaggaaatcg ttcctatttt aactccagca caagcacaag
    18301 gatcgatcta tgaatatttt gaaaatgagc tttctttgac gattgcttac gcgcaaaagg
    18361 agatcacagt agaagaagta acggaaatca tgaaaacagc gatggatctt cacgatgaca
    18421 cttatgcagt agtagtcaga agcctcgttc atctggaaga tacccgctgt ttcgagtatt
    18481 ctgagtcggt tcaccgatta gataaattcc gtttcgttga gtttgcccga cgccgaaaag
    18541 tgtaaatagt tcaaaagaaa gcaaattgtt ggcatctgtc cactttggcg ctatgatagg
    18601 atcacaaagg gggcattttt aatggataaa ccaataagac cagaaaaaga agaactgaaa
    18661 gaaaagttat cacccatggc ttatgcagtc acacaagaaa atgcaacaga acgaccattt
    18721 agcgggaaat atgatgattt ttacgaaaag gggatctatg tagatatcgt cagcggtgaa
    18781 ccgttattca gctctgcaga aaaatatgat gcaggttgtg gctggccatc attcagcaaa
    18841 ccaataacga aaagacaagt gcgcgaaaaa gccgattttt ctcatggcat gcatcgaata
    18901 gaagttcgaa gcaaagaagc agattctcat ctcggacatg tgtttaccga tggaccagta
    18961 gatcaaggtg gtctacgtta ctgcatcaat tctgcctcac tgaaattcat tccatatgaa
    19021 aagatggatg aattaggtta tggggaattc aaatcacttg tagaataatg gaaatacaca
    19081 aagagcggta caatacgggg atttactcga tcgtccgctc tttttttatt caagcgattg
    19141 ctatttgaaa gaaacgctcc ctagactcct tttttcacaa ggtgaaatgt gctacaataa
    19201 agggaacgta tgtgtgcaaa agaaagagga ctagaatatg tatgaatatc tgacaggtgt
    19261 ggttaccttt atcaacccct attacatagt agtagaaacc aatggtatcg gctaccagat
    19321 cgcactagga aatccttacc gctacagtag caaactaaat caaaaaataa aaatttatgt
    19381 ccatcaggta atacgtgaag atgcgcatct tctgtatgga tttgattcgc tagaagaaaa
    19441 gcaactgttt ttgcgattag taagtgtttc aggaatcggt ccgaaaagtg cccttgcaat
    19501 catggcaagc gaagaccact ccggtttgat tcaagcagta gaatccagtg atgtcactta
    19561 tttgacgaaa tttccaggag tagggaaaaa gacagcacaa caaatgatct tggatctaaa
    19621 aggaaaattc ggtgagctga gtatcgatac accttttagt ctatttgatg aagcaaacac
    19681 aaaagacgct acagctctat ctgaagcaat agaagctctc tcggctcttg gttacagtga
    19741 tcgagaaatc aaacgtgtag aaaaacaatt aaaagaagta gataatcaga caacggatga
    19801 gtatctacgc caagcactca agctaatgat gaaaaaataa ggaggagacc aaatgacaga
    19861 tgaatacttg ttatcaccag aatctggcga aaatgaactg agtttggaaa aatctcttcg
    19921 accacagctg ctttctcaat atatcggaca gagaaaagtc aaacaagaac ttcagattta
    19981 catcgaagct gccagaaatc gggaagaacc tttagatcat gttctgttgt acgggcctcc
    20041 tggattagga aaaacgacga tggccatggt cattgctaat gagatgaccg taaatattcg
    20101 tacgacgagt ggccctgcta tcgaacgtcc gggtgactta gtagcaatct tgaatgaact
    20161 ggaagcaggg gatgtcctgt ttatcgatga gatccatcga ttacctagag tggtagaaga
    20221 gatgctttat tcagctatgg aagatttcta tgtagatatc atggtaggac aagggccaac
    20281 cgcacatccg gttcatttcc ctcttcctcc ctttactttg attggcgcga cgacacgggc
    20341 aggtatgctt tctgcacctt tacgagatcg atttggcatc atttctcata tggaatatta
    20401 tgacgaacag gatctgcagg agatcgtttt acgttcttcg gatatttttc aaacagagat
    20461 cattgaagaa ggggcattgg aaattgctcg ccgatcacga ggaacgccta gaatcgctaa
    20521 tcgcttgctc aaacgggttc gtgattttgc gcaagtccaa ggagacggga aaatcgatcg
    20581 tcctatagcg gatcaggcac ttactttgct acaggtcgat caggctggat tggattatgt
    20641 tgaccagaaa ttgttgcgaa caatgatcga gttatatggc ggcggaccag tagggttaag
    20701 tacgctttca gtaaacatag gagaagagcg tgagacggta gaagatatgt atgaaccgta
    20761 tctgatccaa aaaggatttt taaagcgtac acctagaggg cgaattgcta ctgcttatgc
    20821 ctatgaacat tttggttacg actatcatat atgatttttg cttttagatt ggaggaaaat
    20881 gaatgagtga tacaacacgt acgaaacaat tgatcaaaaa cgtattgatt gatttgtgcc
    20941 aaacgcaatc ttttcgaaaa atcagcgtcc aaaaaatcgc aaacgaagca aaaatcaatc
    21001 gacaaacatt ttattatcat tttacagata aatacgattt gcttcgttgg gtctattgtg
    21061 aggatgcatt gcattattta gatccacaat gtctttcact tgataattgg gaagagcaag
    21121 cattgaaaat gctgaaagca attgctgaga agaaagaatt ttatgcaaat actgtgcttt
    21181 ctgacccaga aattttacaa aaagagtttt ctcaactgat ccaaaaatca tttatcaaga
    21241 tttttgaaca aatggatgaa gaaagacaac tttctgcaag agacaaacta ttttatgctc
    21301 gttttttttc ttacggttgc agtggtgtat tagtcaattg gatcacgaat gaatatgaag
    21361 aaacaccgat ggaaatcgct atgcagcttt tccgtttagc caaggatacg gaattatatt
    21421 cttatcgtct ttatgcaaaa gaagagttgg aagaacagta atagaaaaat tttttgttgg
    21481 aaaagcaata atcgctaaaa ggagatgggg aaatgacaaa aattttattt gtttgtttgg
    21541 gaaatatttg ccgttcaccg atggcagaag gcttgctgag aaaacgaata gcattagaag
    21601 gaagggaagc agaatttttt gtggattctg ctgctacaag cacgtatgaa gttggaaaga
    21661 caccgcaccc tggaacgaaa aaaatattaa accaagaaaa agtagatatg acgaatatgg
    21721 ttgctcggca aatcacaccc cacgattttg aaacttttga ttggatcatt ggcatggatc
    21781 aagaaaatgt agaagaactg aaacgaagag caccacgctc agcgcagggg aaaatccatt
    21841 tgtttttaag tagcattcct ggaaaagaaa aagaaaatgt tcccgaccca tactatacca
    21901 ataactttga tgagacatat cacttgatca cagaaggcct gaatcattgg tggaatattt
    21961 ggattgatga aaataaaata acaaataatt tgtaaagtta acgctttctt tttaaggcga
    22021 tgttagagca tttccttgtt tttttgtaaa atgttaggta aaataaaatt aattaagtaa
    22081 cactttctta gctaaaggag gagtgcattc gatgaacgaa gagacagtat cgccaatgaa
    22141 gaagcttacg aagttagtta ctcatacttc gtatcaactt tttgattctt ttgtaaatga
    22201 cactgaagaa catgctaaga atgtacgatt gcaaggaaag tttattcttg agctgcaaaa
    22261 agctgctact gaacatagtc tagttatctt acaagtaaga gcaaatcgtt tgagtagcaa
    22321 gtatgaaacg atctcaggct gggtggcgac aaaaacggtc gaaaaagacc gagtgatgat
    22381 ccgcctacaa aacgaagagc agcagatccg gatggttcct gtagaacaaa ttgaaaaaat
    22441 aacaatcctt tcaccagcag gggaacagga acggttatca aaataattta taccagatag
    22501 aaaagcacgt atgagcttgt accatcaatc attactaatc aaaaataacc gaatgcttta
    22561 agctgatatc agtaaaacgg aacgttttag gatgagtatc atagcatgac agcattcggt
    22621 tgattagttg aatcgattgg atggtacaag tttttttgat aaaaaaaggt cctgctagcc
    22681 ggggagctag caggataagg agttaaaaat gaaaaagtgt tgttagggtt gtttgttggt
    22741 atgcttatat aatagtcatt agttgtgaat attttgtgac gaaagttttc gaaagttgtt
    22801 aagtttt
//
LOCUS       DACDKJ010000044        22399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDKJ010000044 DACDKJ010000000
VERSION     DACDKJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.044"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..361
                     /locus_tag="IV328_001858"
     CDS             <1..361
                     /locus_tag="IV328_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8937995.1"
                     /translation="IETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYF
                     ADPGTPSQRPLNENSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTP
                     IEIFLSYVQEAFYSSLI"
     gene            complement(432..1742)
                     /locus_tag="IV328_001859"
     CDS             complement(432..1742)
                     /locus_tag="IV328_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8937996.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1754..3172)
                     /locus_tag="IV328_001860"
     CDS             complement(1754..3172)
                     /locus_tag="IV328_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8937997.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3412..4980)
                     /locus_tag="IV328_001861"
     CDS             complement(3412..4980)
                     /locus_tag="IV328_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8937998.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4984..5265)
                     /locus_tag="IV328_001862"
     CDS             complement(4984..5265)
                     /locus_tag="IV328_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8937999.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5262..6185)
                     /locus_tag="IV328_001863"
     CDS             complement(5262..6185)
                     /locus_tag="IV328_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8938000.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6182..7060)
                     /locus_tag="IV328_001864"
     CDS             complement(6182..7060)
                     /locus_tag="IV328_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8938001.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7047..8384)
                     /locus_tag="IV328_001865"
     CDS             complement(7047..8384)
                     /locus_tag="IV328_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8938002.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8387..9490)
                     /locus_tag="IV328_001866"
     CDS             complement(8387..9490)
                     /locus_tag="IV328_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8938003.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9564..11072)
                     /locus_tag="IV328_001867"
     CDS             complement(9564..11072)
                     /locus_tag="IV328_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8938004.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11038..11730)
                     /locus_tag="IV328_001868"
     CDS             complement(11038..11730)
                     /locus_tag="IV328_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8938005.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11752..12726)
                     /locus_tag="IV328_001869"
     CDS             complement(11752..12726)
                     /locus_tag="IV328_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8938006.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13026..13898
                     /locus_tag="IV328_001870"
     CDS             13026..13898
                     /locus_tag="IV328_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8938007.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14480..16678)
                     /locus_tag="IV328_001871"
     CDS             complement(14480..16678)
                     /locus_tag="IV328_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8938008.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16910..17902
                     /locus_tag="IV328_001872"
     CDS             16910..17902
                     /locus_tag="IV328_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8938009.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17962..19383
                     /locus_tag="IV328_001873"
     CDS             17962..19383
                     /locus_tag="IV328_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8938010.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19376..20875
                     /locus_tag="IV328_001874"
     CDS             19376..20875
                     /locus_tag="IV328_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8938011.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20914..22308
                     /gene="uxaC"
                     /locus_tag="IV328_001875"
     CDS             20914..22308
                     /gene="uxaC"
                     /locus_tag="IV328_001875"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8938012.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6797 a         4564 c         3879 g         7159 t
ORIGIN      
        1 tattgaaact gcccttaatc aatggttttc tcgcttccct aaaaacttct ttaaatctat
       61 tacgtttgac tgtggaaaag aattttctaa ctggaaagcc attagtaacc aacatgatat
      121 tgatatatat tttgcggacc ctggaacacc ttctcaacgc ccattaaacg agaattctaa
      181 cgggattctg cgtcgtaatg gactgccgaa atcaatggat tttagaaaag tgaatcagac
      241 atttatttcc agtgtcagca atcaacgtaa tcatattcca agaaaatcat tgaattacag
      301 aacaccaatc gaaatatttt tgagctatgt acaagaagca ttttattcta gcttaatttg
      361 acaaatcata tatcattaaa aaaaataaag gtcaagctaa aatattattt aagcttgacc
      421 ttattataat ttcaacgatt gtcaaaagta ttgtttgcag ctgtgttgcc agtttctata
      481 gataaatcac agcttttgaa acataagttt tgtactgctt cgattcgccc gaattctttt
      541 gcagttattt ggcaatgaga aaagcagaca tcttctatag gcttttcagg aaatgctact
      601 aaatcaaaag ctcgggaagg aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg
      661 atgtgtatgt ctttgacttt cgtcatttgc tttgattcag gtatttcttc agccactgct
      721 tgccaacttt ctggcatttg agcaaggtca ttgctatttt ttctattgta ttcattatgc
      781 cagtccatta tccaagaaaa aggaaattgt acatcaatca tattcagatt ttctaccctg
      841 atattttcga tcactccccc tctttctttt gaagatttca ttcgaatacc acagtctgac
      901 tgaaaaaaat tgatatcgtg aatatatacg tcactaactc cagcactaac ttcgctacca
      961 atagttacac cgtatcctga acggatttta catctagcaa cctcgattct tgatgatttt
     1021 cgattgactc ttgcaccatt gccatctctg ccagacttaa tagcaataca atcatcacca
     1081 caggccaaat cacattcata gacacgcaca tttgttgaag aatcaatatc aatcccatct
     1141 gtacttggtc catcattgtt gcggatgatt actttttcta ctaatacatc gttagaataa
     1201 gtgatttgca agttccaaaa acctgatttc tgaaaaatca aatcacgaac aacaacatgt
     1261 tctgcatgat acagtaaaca agcgcgtgga cgtttaatag cataatccgc aatccaacga
     1321 agtccctttt gatcgtattc agctcttgtc ccgctttttt gatcttttcc ccaatacagc
     1381 tcccaccaat ggtctccttg accgtcgatt attccttttc cttctataag aatatctttc
     1441 gccgagagaa cattcaaaat cgctgctggc catttcattt ctacgccggc tactcgtgta
     1501 tcgatttctg gaaatgcttc gatatctgtt ctccccttga ggactgcacc ttcttcaaag
     1561 ataagatgac tcttatcttt taaaaaaagg gctccgacta aataaatgcc ttttggaata
     1621 aatacaggta agtgttcttt cgcaccttga tcgattgctt tttgcagtat tattgtatca
     1681 tcttgttgag tgtcgtttgt tttaaagttc aaagcattga taatcccttt ttttgtccgc
     1741 aatacgtttc tccctatctt tcattgattt ttttcaattc ttcaccaata aatacagttt
     1801 ttgaacgatt cagtgggtaa cctactagca aaatgattgc ggatacaaat aagcagaatg
     1861 ctggtaacaa gtttgccatc gtataaatcc tttcttgtac taaaactgat tggatggctc
     1921 cacctgttgt agacgattgg taaccaatga gtgccagcat aaatccgcct accgctcctg
     1981 cacatgcttg tcctactttt ctcgcaaaag aatttactcc atatactgtt ccatcttctc
     2041 ttaagcctgt cttatattga tgataatcaa tcacgtcagt aatgaatgcc cagaccatca
     2101 aattgaataa accagatcct aaagtagcga taaataaaag aatcagatat acccatgcat
     2161 ttgttatatg aaggaaatac atcagcaagt acatacatga agaagcaagt aacgcaaata
     2221 cgcttgcttc ctttttacca aataattttg tcagctttgt agcaaatggt gctaaaacta
     2281 ctactgttcc gtaagtaaat aaaagagcaa cagacattgc ttgtttatta tggaagtaat
     2341 cattgaataa ataagtcata ttcgtcccag ctaaaatctg attgatcaca atgaaaatgt
     2401 caacaatgac taaaataatc agtgctcgat tagctaacaa accttttacc aactgagaag
     2461 cagatacttt ttcgtttttg acaactctta ctctttcagt agtcaattta caagttaaca
     2521 tatatgcgac aaatgctatt gcagcacatc cgattgaaat ccaaaagaaa tgattccctg
     2581 aaacgacttg ttgtccactc ttgttatggg tatacattaa aatcggaatg aagaatcctg
     2641 tagttgatcc gccaatcgca gaaccaatgc ttcggaacgt ggacaacgag gtgcggtcat
     2701 taggatttga gctaattgct gctgccattg ctccatacgg gatattgatt gttgagagaa
     2761 aaatcccata aacaatgtat gaaacaaaaa tataagcaat cttcaccgtc attggaagat
     2821 gttggacgaa tggcaaaaac aaaatgacag tcactaagca aaatggatac ttcatttttc
     2881 tgatccaagg aataaatcgg ccagtttctg ttaactctga gacatcacat aatcttccaa
     2941 ccatcacatc agcaaacgca tccaaaattc tcgaaatcaa gaataacgta cctacgattg
     3001 cccctgaaac gccaagaaca ttcgtataat aaatcatcaa gaaactattg actaaaccta
     3061 aaataaaaca gttacctaga tcaccgaaca tgtagcctat cttatcggaa atcccaaatt
     3121 cctttttttc tttttttact gtatttgctg taacagcatt ttgtgaaagc atttgaattc
     3181 ctcctacctc ttctagtaaa cgttttcaaa attataactc tttttttctt gtttttatat
     3241 tcacaatgtt ggctttttat tgcattatct tgacagataa aaaacaaaaa ataatctaaa
     3301 aacattgatg cataagattg ataacttttt ttacataaca atatatacaa acaaaaaaat
     3361 tcactaacta gtgaaaataa gttagtgaac ctcatttatt aaagaattaa attacagatg
     3421 atcttttcca tcatacttga aaaactcaaa atcagcataa cttcctcttt ttttatttag
     3481 atcatgcact gctatcccga taaaatttcc agtaaaccct cctgagagta tacacatgtt
     3541 tttttgcgga cctactttct tccattcttc agttgatacg tctttcaaat gaaactgtgc
     3601 tgtctctttc tttacagtga ctttcaaaaa agtcttcttt tctgtgagtg gaatcgtcca
     3661 atcttctatt tgtgctactc cgttttctga ttctatcata cgtaggcatt ttcccttgtc
     3721 ctcattccaa gtaacataac aataaatata attggattca tttaagtaca gtaccagtcc
     3781 agccatctga ttaaaattat taggcatgta ttcaaatgct gtctccgcat caaaagtaaa
     3841 atccgtttgc cgaatagcta acaaatgatg ttcgaaggta gattgaagag attcaccgga
     3901 aatcagacgt aaaaaacctg gtctacattt tgtatcacac caatcagcat ttggcaaaat
     3961 gcgtctggca ttccagttct tctttagctc tccttcaaat gtatccataa actgatgatc
     4021 tttttcttgt tgtacctctt catttgtctc aatcattatt tcttccatcg gagttgtctg
     4081 gctatcttta agtcttaacc aatcgtcgga cgtccaatac acttcttgaa tagcggtttc
     4141 tcttcccaaa atagccgcac cgtttaatgg tctggtacaa agatatgtca tgtaccatct
     4201 tcctgacggg gtttgtacta agctcccatg tcctgtacat tgcaatgtag aagcaggttt
     4261 gtcactagct gtcagcatag gaaaaccagg atcaagctcg taaggaccag taatattctt
     4321 tgctcggcaa actgtaactg aatgtcctct tcctgtaccg ccttctgccg tcatcaaata
     4381 ataatacccg ttacgaaaat acaaatgtgg tgcctccgtt ttcgctaatt ctgtacctgc
     4441 aaatatttta tagatgggac catcaagctt cttcttctct gcatcatatt cttgtaatac
     4501 gataccgctg gatttattcg gtgtgtccag acgataatcc catagttcat tgacaagcca
     4561 tttttttccc gtttcaagat catggaacaa agaaggatca aagccgttac tatttaaata
     4621 aattggttcg gaccaaggac ctgtgatctc agatgcagtg atcaaataat tgtggacatc
     4681 tttaaacgga cggcctgcac ttttgacatc tgtgtaaaca agataaaaca atccatcagc
     4741 ataactgatc tgtggcgccc atatacttcc atttgtagga tttccttgca aagatacttg
     4801 agtggtcaaa atatccgttg tatgtgacca gttgactaaa tttttcgact catatactcg
     4861 gattcctgga agccattcaa aagaggagac aataatgtaa taccgctctt tcactcttaa
     4921 tatattagga tctggattga atcctcttaa aataggattt ttcactttga tagtctgcat
     4981 gattcaggct ccttttctca ttcttactcg atataaactg aatgccccag ctgctaaaaa
     5041 agcaagtgaa ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa
     5101 tgtattttct aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa
     5161 ctctgacgca gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag
     5221 actgccaccg accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc
     5281 ttttctaaat gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca
     5341 gagaatttga cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga
     5401 acatgcccac ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt
     5461 cctaaataga cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac
     5521 cctgtttttt gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct
     5581 aagctatgga tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc
     5641 gttccttcaa tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc
     5701 ggtgtacaaa aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta
     5761 tcctgataac cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa
     5821 gcaacagcct gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct
     5881 aatacgacat tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta
     5941 ccgtcaagat actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc
     6001 acaagtttaa aattggataa tctgctttct atatattcat catatcttcc ttcttttact
     6061 atcaagacct ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata
     6121 gtttcaaaat ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc
     6181 ctcataaatg ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat
     6241 gaccgcctgt taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt
     6301 tgtccagccc ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc
     6361 caactaagct aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag
     6421 ctgtcaatag atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca
     6481 aaagtgtttc gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca
     6541 atgctgtcac ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt
     6601 gagtatctgt gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac
     6661 gcattcccca taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa
     6721 tacctccgcg ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg
     6781 tcagtacttc tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt
     6841 tcccgactga aaaatctccg ccgtgagaat gcaggtaaag gaccgtcgga aacggtcctt
     6901 ctttttctgg ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat
     6961 agtattcaag cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat
     7021 gtctgctaga tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca
     7081 accagttcat ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta
     7141 gtattttcta atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa
     7201 accttacccg caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc
     7261 atagcaggaa caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg
     7321 atttcttgta tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc
     7381 cttgccgtga tattcgaaaa atggatcctt ctgaatgctg atgtccgttc atctatcgga
     7441 taagcctttt tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga
     7501 ttcagtataa atggacacat cacattatcc atgactatat tggatactcg aatatcttca
     7561 acgatcccgc ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag
     7621 atacaatttg aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca
     7681 ccgccatgtc catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt
     7741 tcgtacgtat cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg
     7801 caattactaa tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa
     7861 tccgctgggt tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg
     7921 atcgtccaac ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta
     7981 tggaaactca tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta
     8041 tgccaccatt tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct
     8101 acattttgag catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa
     8161 tgaacaacag gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa
     8221 tgaagttcga cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca
     8281 actacggtcc cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta
     8341 ttcaattcat caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca
     8401 cttcaatcaa tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt
     8461 cactgatgta gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt
     8521 gacagttttt attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta
     8581 atccttgatg tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac
     8641 gtattccttt agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc
     8701 ctttccgttt tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg
     8761 gaagtaatgc agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt
     8821 catgaagtaa ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc
     8881 aaaaatgatg ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc
     8941 atgcatgaat cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact
     9001 gtctcaaaac atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa
     9061 aaggagaacc catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc
     9121 aaaaagctcc ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg
     9181 cttttttata tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat
     9241 ttacatggtc gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca
     9301 cgaaatagtc gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc
     9361 aaagtaattg gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc
     9421 acttttcttc atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc
     9481 agttttccat tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga
     9541 agtatttgtc ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct
     9601 tgtgtgattc gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct
     9661 tcgtcaagct gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct
     9721 gatcctactt cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat
     9781 tcttcatttt ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa
     9841 gagaacgtca cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga
     9901 tccatatcga taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata
     9961 ccgccagtca tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat
    10021 ttcttcaatt gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata
    10081 ccatttcctt ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca
    10141 ggcactaatt ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc
    10201 accattccag tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt
    10261 tgggtaactg caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc
    10321 aaagctttca tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca
    10381 gcccagccat ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata
    10441 tcactaggcg cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg
    10501 ttttctgtaa attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt
    10561 cctagattat ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt
    10621 atcggtacgc cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt
    10681 tgacttaaat ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt
    10741 aacgcattgc ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct
    10801 agttcatcag aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag
    10861 ttgaatgtca gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc
    10921 ggtgaagcat gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct
    10981 gaagaactgt tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta
    11041 agacctaatt ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat
    11101 atttttgttt tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat
    11161 agaatttgct gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac
    11221 acctggcatt gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa
    11281 tgagtaatga agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc
    11341 tttccaatca taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa
    11401 cgcagcaata tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag
    11461 taaaacaatc ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc
    11521 tttaaaatat gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt
    11581 tctaatcact gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc
    11641 gatgactaaa ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct
    11701 tccgtttatt ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac
    11761 gccgtcttct cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac
    11821 gaccccttta aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc
    11881 tgtataaata taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa
    11941 gtacatatgc tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat
    12001 gattactgga cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc
    12061 atccatttct gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat
    12121 gatcgtagac cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca
    12181 ttcaggggat gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa
    12241 tacccctccg tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat
    12301 gaagtgaggg atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc
    12361 atttaatagt aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa
    12421 caagatcaac gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa
    12481 atgtttgaaa ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa
    12541 agcaataacg agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg
    12601 cagaatcatc acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct
    12661 tctgagcttt tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact
    12721 ttccataagc ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa
    12781 ctatctgaat tacgtatata atcattttta tcgtaagtaa aaacgcttac atagcaatga
    12841 tttttgtgct atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa
    12901 ccttctattt ttttctatct gacacttagc ataggaaatg attatatacg taatctttca
    12961 ccgatgataa attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta
    13021 tctatatgaa aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt
    13081 ttctaatttt gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt
    13141 ttgcttctac acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt
    13201 tagacgcgta tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga
    13261 ttggtgttac aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg
    13321 gtctttccag aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta
    13381 tgttgttcag cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca
    13441 attcttactg ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga
    13501 gaaacttttt ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca
    13561 cagattttgg tgtatttttc aaaattatgc tgccattagc tttaccgtct attgctacta
    13621 tctcgctttt ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca
    13681 acgattcatc taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg
    13741 ggatgcaagc agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg
    13801 ctgttatcgt tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt
    13861 ttgtcaaagg agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg
    13921 tagttgtgtc agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct
    13981 taaatttttg tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat
    14041 cagaggttgt gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc
    14101 gacgagtaac ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac
    14161 aaaattatgc cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa
    14221 ctgtgacaag acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc
    14281 ataaatacaa atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa
    14341 aaacagcctc ttgattaaac ggcgtttatc ttgttgattc tgcggcaata aaatcagcca
    14401 gcagcaaaac atgaagtgct gttttttgct gtcctctttt attgcttgaa tcagatcaca
    14461 agaaaaacag cctcttgatt cacaaatatt tttttcgata ttctcccggc gtaatcgatt
    14521 ccttcttttt aaagaaacga ataaaatttt gcggatttcg atattgcaat ctttctgcaa
    14581 tctcttttac ggagaggtta gtctctttca accatttttt tgctacctct aaacgatgat
    14641 tttgaacaaa atcaccaaat ttttctcctg tttccttttt aaatacatta cttaaatagt
    14701 tagggttata atgcaaccta tcagcgatca catctaaaga cagatcacga tcatattctg
    14761 tgtagacgat atgcataata ttctccgaca aatttttaaa ttcatgatca gtgattgctt
    14821 gagagcattc tgtgattggc agaactaatt gttctaaaac aagtttttct agttctttgg
    14881 gatgataatt gttcagtgct tttacataaa tttgtttcat gttatcaaag ttttttgaat
    14941 ctactcccaa taactgtcct aattgaacga gttcattgat caagcggacc gaaactactt
    15001 cccgactcaa aggattttta ttttgtgcaa acaatgcatc aatcaattca tgtactaaat
    15061 tcttcacttc ctgatttcca gaacgaatgg cttcaaacaa tcgattttgt tcttctatcg
    15121 gatatctaat aagtgttttt tcatgagcag caggaacgat ttcctgataa aaaataattg
    15181 aacttggacc agtatttaca cgataataca gggcttcttt tgcccgatca accgattgct
    15241 tgctttcatt cagcgtttcg aaccggttac tgatgcctat actgatagtt aaattcaagt
    15301 attcttttac acgtttttga atgagtcggc aatactccat gacttttttg cttgcttctg
    15361 catcatcttt atccattcga taaatagtgg cttgcatttc ttcattcaaa acgatcggca
    15421 gcattcgatt ttcctttggc acaatttccg tgatcatgtt gttgatcgct aaaagtagca
    15481 aatcacgctc cccaccgtga ctatcttcta acagatcgat ttgaaccaaa gctgtataat
    15541 agcagtcatt ttgagaacta taaccaaatt gctgcaaccg ctgatctagt tctcgccttt
    15601 ctactcgatt tctaaataaa ctaagtacaa atagcgtttc cagctgtggc ttttgggtaa
    15661 ctaagtgtgc acttaacgct tctttttctc ctatgatccg actgactgat tgagcgacca
    15721 aatctagttc tttcccactg aatccttttg atttaagatt cagacgttcc tgtatttgag
    15781 aaatcggacg ggcaaatctg tccgaaaata agtaggaaaa aagaccaatg agtcctacca
    15841 gtgttaaaga cactgcaact agcccgattc tcagattgcg gatcattgcg ccaattgcac
    15901 tagagtcaac ttctaatctg taaatccaat tattataatc tgatttcagt actatatacg
    15961 ttttatcttt ttctcttatc actttcagct gattgtaatc tagatccacg tccgttaata
    16021 cagttggttg ctctttattt tttactgtct gagtagtaaa aaggttgcca acttcgtttt
    16081 caatagaaag tatgtcttct ccactttcac caacaacttg ttctattgta tgtttctcta
    16141 tcgtagcaat tcccaatgca tatttttctt tttcaaacat tggcaaagaa atcaccattt
    16201 cgattccttt gtttttcttt tgccaaaata aattattttt gttatttatg tacttctgtt
    16261 gatacttttc aacttcctct tcggtcagtt gactcaacga tccattgatt actccccatt
    16321 taccagccaa actgactaaa tcataattat tatcatccat aacaatcgtt tctataaaat
    16381 ttagttcttt cctgatttca gaataagtgg agtaatctgt atatgtcagc ggatgcttga
    16441 atatgtccga gaaatttttg gtcgaaccat acgtagaaaa cgaatattca atcgttctaa
    16501 ttttccattc aacgttagtt ttaacttgcc tgagcaaatt ctttttgtct gttaaataac
    16561 tcgattcgat cgattcatag gtattctggt aaatgaataa actaaatcct atgacaagta
    16621 cggctcctaa taaaaacatc ggtaaaaata ttttataaaa tactgtactt ttcttcattt
    16681 tcttcctctc ctccttttaa aataaaaggc tttcattttt ctgtaaaatt gatactcatc
    16741 taattaaatg agcattcccc gctttcaaga atagcataat taaaaataaa aatccaagta
    16801 atttttaacg tgtgcaatct tttcatatca aagatctatt gacttatcgt agttatcgta
    16861 atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa aatgatggaa tggtaacgat
    16921 caaagatatc gctaaagcag ctggcgtttc tcatacgaca gtttctcgtg ctttgaataa
    16981 cagcagttta ataaaaaatg aaacaaaaca aaaaatacga caactagcta aggaaatgaa
    17041 ttattcgcca aactatagtg caaaaggttt agtgaatcaa aaaaaatatc taatcggact
    17101 atttttctca agtttgcata aaggaacttc atcaaatttc ctagcagatt ccattgcagg
    17161 catccattca gtattagaca caaattacag tttatctgtt gaaagtatcg atgttgttga
    17221 tttaacagaa atcaacctgc aacgctacga tggactgatc gttatgagtc aaagtgatga
    17281 tgatcagcct ttcatcgact atctcaaaga aaaagaattt cctttcgttg ttgtcaatcg
    17341 tcacatcaat gatccagcgg ttatcaacgt cactgccgat gatgcggccg gtgttatgca
    17401 ggcagtagat tacggtattg gatgcggaca tacgaagatc gcttatattg gcggaaagaa
    17461 aaattttcgt tcgacaaaag aacgggaaaa aggggtattg aaaagtctaa aaaaagcgag
    17521 catccctgtc aatctagcct attttttaac aggagattac agcttagaaa gcggcctcca
    17581 aaacatggaa tacctattat cactttctga cctgcctact ctggtttttt gcggcaacga
    17641 cgacatagcg attggtgcat tgagagctgt acatttagct ggcctccgtg ttccccagaa
    17701 catttctctg atcgggttcg atgattctcc agtcgtctcc tatctcaatc cgccgcttac
    17761 caccgtacgt aagcctttga aagaaatgtg tcaagaagga acaaagcttt tactagattt
    17821 gatcgaaggc aggacgatcg aacaatctaa aattgagctc tccaccttgt tgatccttcg
    17881 tgagtcgatc aattacttgt aaactacaag taattttata taaaaatgtt aacgtgtgca
    17941 taacacagaa aggaagttta tatgaaaaaa ctgacaaaag aattagtgaa aaaaaacgaa
    18001 gcacctgtaa aagttcttca gtttggtgaa ggcaatttta tgcgtggatt tattgattgg
    18061 cagattcagc agctgaataa ccaacaattg tttaaaggta atgttgcaat cattcaacct
    18121 ttagaacaag gtcttggaaa tatgatgaac cagcaagata atttatatac ggtcatttta
    18181 caaggactca gcaatggcga aatcgttgat acttccgaga ttatcaccgt agtagaacaa
    18241 atgatcaatc cttatgatga atgggatgct tatctatctc tagcagaaaa tgacgacttg
    18301 gcctatatcg tctccaatac aacagaggca ggaatccaat tcgtggagac tgataattta
    18361 acagatactc ccccacagag cttcccgggc aaactgacag cctttttata ccgacgtttc
    18421 aaattaaaca aagctggctt taccatcatt ccttgcgaac tgattgacag aaacggcgaa
    18481 cgtctaaaag aaatcatttt acgctacgca gaaaaatggg atttagaacc tgcttttgtt
    18541 gagtggttag aaaaagaaaa tgttttctgt tgtagtttag ttgatcgcat cgtcccaggt
    18601 tatcctcgcg aagatgcagg tgttttcaat caacggcata actatgaaga tcaattgatg
    18661 gtcaaagcag aaccattcat gctttgggtg atcgaagggc ctaaagaatt ggagaatcaa
    18721 ctaccattca aacaggctgg cttgaatgtc atcatcacag atgatatgac tccttatcgt
    18781 gaacgtaagg tgcatttgct taatggaccg catacagcga tggtccctct tgctttgttg
    18841 gcggaattgg aaactgtaga agaagtaatg aaagatcctt tattctccgc atatgttgat
    18901 cagttattca atctagaatt gattcctatg ctttctttac ctaaagatga attagccatt
    18961 tatgctgacc aaatcaaaga acgtttcttg aatccttttg cccatcacaa attagaagcg
    19021 atttcattaa atagtgtttc aaagttcagc acccgtctgc ttccagtgtt caagaagtat
    19081 atcgaagaac aaaatcaggt accaccgtta atcactgttt cactagctgc gctacttctt
    19141 atgtatcgag gcgatcaagt aaagccacat gatgatgaaa agacgataag cgaatttact
    19201 gatgcttggt ctgataatgg aacagctatt cctcgattat tgcaaaacgc tgcgctttgg
    19261 ggagaagatt tgtctcaaat accgaatgtg acagatacag ttcaagaaat agctgcaaag
    19321 atcgatcaaa aaggtgtccg gtcagtatta caagaaatga aaggaacgat ggtccatgct
    19381 tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac agtgtggtcg tcgcattaac
    19441 ccctattcca aaacaaacac aactgacgat tgatcagcaa acgatcacca ctcttgaaga
    19501 tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa gctggagaca acgtgatcaa
    19561 atatggctat ccaatcggtc atgtgacgac agacgtaact gccggtcaat ggctgcacac
    19621 tcataatgta aaaaccaatc tttctggtga attggattat cgatatgaaa aagatgtcca
    19681 tccaagtcat tacctttttg agaaccgaac atttcaaggt tacttgcgaa aaaatggaaa
    19741 agtagggatt cgtaatgatt tgtttttagt acctaccgtt gggtgtgtga atggcattgc
    19801 tgaactgatc gtcaaacagt tcaaggaaaa acatccagat ctgggacagt ttgaccatat
    19861 tacgatttta aagcatcctt atggctgttc gcagcttgga aaagatcatc aaaatactcg
    19921 agaaatttta gcggacgcag taaatcatcc caatgccgga ggagttttag tatttggatt
    19981 aggttgtgag aacaatacag ttcctgaatt caaaaaaatt ttaggtgaat atgatgaaga
    20041 acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag atcgaacaag gtgtggctct
    20101 attagaagag cttttaacag cggcggaaaa tgaccagcgc atatcagtcc cgctctctaa
    20161 gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta tcgggaataa ctgctaatcc
    20221 tttacttggt gcattttctg actacttgat tgctcaagga ggcagcacga tcttaacaga
    20281 agtgccagaa atgtttggcg cagaacaagt attgatggca cgagcggaaa atgaagaagt
    20341 ttttgactca atcgttgact tgatcaatga cttcaaacag tatttcttgt cttacgggga
    20401 accagtttac gaaaacccat ctcctggtaa taaagcaggt gggatcacta cattagaaga
    20461 taagtcttta ggctgcacgc aaaaatcagg aagctctcct gtcgttgatg tgctacaata
    20521 tggcgaaaaa atcagaaaac cagggcttag tttattgcaa gctcccggga atgatcttgt
    20581 agctgcctct gctcttgctt catccgattg ccagttggtc ttgtttacca caggaagagg
    20641 gactccgttt ggctcatatg tcccaacggt caaggtatct acgaatacaa ctctttttga
    20701 acgcaaaggt cattggatgg attttaatgc aggcgtctta ttagaacaac caatggaagt
    20761 aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct agtggagaag aaacgaaaaa
    20821 tgaacaaaat gacgttcgag aaattgccat ttttaaaaac ggagtaacac tttgattttc
    20881 ataaaacagt ataaaaagaa agaaggaaga tttatgtttt tatctgatga ttttttatta
    20941 aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa aaaacatgcc cattattgat
    21001 tatcactgtc acttagatcc aaaagaaatt tacgaaaaca acaatttcag caatctgaca
    21061 gaagcttggt taggaggcga tcattacaaa tggcgcttga tgcgagcttg cggcgtgcct
    21121 gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat ttttagcttg gtgtcaaacc
    21181 gtaccaaaaa ttatcggaaa tccgctttac agttggtcac atcttgaatt gaagcgtttc
    21241 ttcggtattg acttgttgat aaatgaagaa aatgcagaga aaatttggac acaagcaaat
    21301 gaaattttgg cagctcctga ttttcgccgt cgggaaatcg caaaaaaatc gaatgtagaa
    21361 gtgatttgta caacagatga tcccgcagac gacttgcact atcatcagtt gcttgctcat
    21421 gaagaaccag atttgaaggt tctaccttca ttccgtccag ataaagcact caatattgaa
    21481 aaagaaggat ttaatgcatg ggtcacacta ctggaagaag cagctgacca aacgatcacc
    21541 acctatcaag aactgataga agtcttaggg cagcggatag aatacttcca tcaacatgga
    21601 tgtcgtgttt ccgaccacgc tttagacatc ttacgctata aagaagcgga tgaaacagta
    21661 ttagaagaga tttttcaaaa agcacgagct aacaaactat tatcttcaga tgaaatcgat
    21721 gcttatctta cagaaacact gatacatttg acccacttct atcattctca taattggacg
    21781 atgcagctgc acatccatgc ttatcgtaac tgtaatacac aaatgttgga aaaattaggg
    21841 cccgataccg gctatgatgg aatgaatgat ctttctttga ctattcctct gcaaaaatta
    21901 ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga tcctttattc attgaatccg
    21961 aatgactatc ctgcgatttt agctttgatg ggctgtttcc aaaaagaaca gaatggaaaa
    22021 ctgcagttag gttctggatg gtggtacaac gatactcgag ctggtatgcg cgatcaattg
    22081 acacaatttg ctgatggtag tgctttgggg aattttgtcg gcatgctgac agattcaaga
    22141 agtttcctgt cctacactag acatgaatat ttccgtcgtg ttctttgtga atttttaggc
    22201 gaaatggtca tgcgaggaga agcacctgaa gatgaacaac ttttaggaag tctagttcaa
    22261 gcgattagtt atacaaatgc taaaaattac tttggctttt ttgcctaatt gattaatagt
    22321 aacagtaacg aatcaaaaac aggatagtga aataacggaa tccgtttatc cacgttttag
    22381 agaagaagac ggaccaaat
//
LOCUS       DACDKJ010000045        22376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDKJ010000045 DACDKJ010000000
VERSION     DACDKJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.045"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(369..4022)
                     /gene="rpoC"
                     /locus_tag="IV328_001876"
     CDS             complement(369..4022)
                     /gene="rpoC"
                     /locus_tag="IV328_001876"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8938013.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(4101..7718)
                     /gene="rpoB"
                     /locus_tag="IV328_001877"
     CDS             complement(4101..7718)
                     /gene="rpoB"
                     /locus_tag="IV328_001877"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8938014.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8446..9414
                     /locus_tag="IV328_001878"
     CDS             8446..9414
                     /locus_tag="IV328_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8938015.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9432..10337
                     /locus_tag="IV328_001879"
     CDS             9432..10337
                     /locus_tag="IV328_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8938016.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10334..11140
                     /locus_tag="IV328_001880"
     CDS             10334..11140
                     /locus_tag="IV328_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8938017.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11297..12265
                     /locus_tag="IV328_001881"
     CDS             11297..12265
                     /locus_tag="IV328_001881"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8938018.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12582..13106)
                     /locus_tag="IV328_001882"
     CDS             complement(12582..13106)
                     /locus_tag="IV328_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8938019.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13393..15204
                     /gene="scm"
                     /locus_tag="IV328_001883"
     CDS             13393..15204
                     /gene="scm"
                     /locus_tag="IV328_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8938020.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15359..16345)
                     /locus_tag="IV328_001884"
     CDS             complement(15359..16345)
                     /locus_tag="IV328_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8938021.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16368..16934)
                     /locus_tag="IV328_001885"
     CDS             complement(16368..16934)
                     /locus_tag="IV328_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8938022.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16948..17364)
                     /locus_tag="IV328_001886"
     CDS             complement(16948..17364)
                     /locus_tag="IV328_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8938023.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGNYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17821..19755)
                     /locus_tag="IV328_001887"
     CDS             complement(17821..19755)
                     /locus_tag="IV328_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8938024.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            20057..20992
                     /locus_tag="IV328_001888"
     CDS             20057..20992
                     /locus_tag="IV328_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8938025.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            21082..22185
                     /locus_tag="IV328_001889"
     CDS             21082..22185
                     /locus_tag="IV328_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8938026.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            22198..>22376
                     /locus_tag="IV328_001890"
     CDS             22198..>22376
                     /locus_tag="IV328_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8938027.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT         6613 a         4760 c         4341 g         6662 t
ORIGIN      
        1 ttttttaaac acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa
       61 acataaatag aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata
      121 atagctagca aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca
      181 aaaaatgatc tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata
      241 ccttaattgg atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac
      301 aaattatttt agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt
      361 taaaatatct attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata
      421 tctgaaatac tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga
      481 taacgttgca taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa
      541 ccaagaagag gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct
      601 tggaatgatg cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt
      661 aagacaggtc gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct
      721 ttaaagtctg cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt
      781 acacgaactt tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct
      841 accccttgca tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat
      901 acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca
      961 cggtggatca tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta
     1021 cgtgtatctg tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag
     1081 atatcgatga catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct
     1141 tcaaagattt cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct
     1201 gtatggaatg tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc
     1261 gtaccaactg cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa
     1321 tgtttgcaga caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg
     1381 acacctgcat caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg
     1441 atcaattcat ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga
     1501 tcttctaatg attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg
     1561 tcagtaccgc aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga
     1621 gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg
     1681 tgagttgaga taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc
     1741 aattccatga ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta
     1801 aagttggaaa tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct
     1861 tccaaacctt ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg
     1921 acacgttcat aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt
     1981 tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca
     2041 gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg
     2101 tcaagcatct tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt
     2161 cctaagtttt tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata
     2221 tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt
     2281 ggttcattca gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact
     2341 gttgtgatca tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta
     2401 gggttcacac cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct
     2461 tcgttttgat ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag
     2521 tagtagttac ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg
     2581 ttcaagatgt tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc
     2641 aatggaacgt ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca
     2701 agtggatgca aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca
     2761 agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct
     2821 aaaacatccc atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt
     2881 gaagctagtt cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc
     2941 atttcttttg gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa
     3001 cgacctgagt agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct
     3061 ttcaacatat gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg
     3121 cgacgaccgt tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga
     3181 acgatgattc caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt
     3241 acacgacggt aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc
     3301 attgggcgta aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga
     3361 tttcctgaag cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt
     3421 tgtccttgtg cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca
     3481 ttatcaagta attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat
     3541 tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta
     3601 tttcctggtt cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg
     3661 ctcatgtcta atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa
     3721 acaggagctg ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact
     3781 tccactccgc atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag
     3841 gcacattccc agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcacgttca
     3901 ggttttaagg tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt
     3961 ctgatttttt cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc
     4021 aaaaggggtt ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc
     4081 cttgcagctc atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg
     4141 acatcgtctt tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt
     4201 tgctgttcgg caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc
     4261 atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt
     4321 gattgcaatt ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt
     4381 tcacccttga cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat
     4441 gtcaagattt cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt
     4501 tcaccaaaac gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat
     4561 gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc
     4621 atgtacatca caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac
     4681 agaacagttt tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca
     4741 tcacttgcac catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata
     4801 cccaagtgta attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc
     4861 agcatgatat cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata
     4921 cgggatacaa cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta
     4981 cgtttttgaa cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca
     5041 gcttcacgag taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt
     5101 aatgatgtat cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct
     5161 tctgcagata attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct
     5221 tggacttctg caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc
     5281 ccgacattag ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct
     5341 gattcatatt cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc
     5401 atgatgatcg catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt
     5461 tggcctagag ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt
     5521 tcgactttct cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa
     5581 cgacggaatt ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt
     5641 tcagaagcat caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag
     5701 tcatgcgctg atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac
     5761 ggcactgctt gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc
     5821 aagaaaggaa tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag
     5881 tctactttat caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct
     5941 tgagcaaatg ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct
     6001 tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca
     6061 cgacgataag gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta
     6121 ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg
     6181 gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct
     6241 aaggctgaaa gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac
     6301 tgtgacaact gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg
     6361 atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca
     6421 cgttccatac gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg
     6481 atacgacgat ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc
     6541 aagaaatagc tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct
     6601 ggatagccgt ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg
     6661 atgacttgga cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact
     6721 gaatttaggc cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact
     6781 gttccttttt caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc
     6841 agattcaata gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt
     6901 gctaagtcgt aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct
     6961 gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt
     7021 cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg
     7081 tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg
     7141 gttaaaggaa tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt
     7201 tccatttcta gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg
     7261 tttttgtcga cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt
     7321 actcgctctg ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg
     7381 aagaatactt cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca
     7441 tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt
     7501 tctttcaatt cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc
     7561 aaaatgtctt caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt
     7621 tgaatttcga tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt
     7681 tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt
     7741 cattcaaaaa gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta
     7801 acaacagaca ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg
     7861 aaaccttact tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata
     7921 tttcgtttct gccttttctt catcagatag tttttgcaac ttcatatact acacttattt
     7981 tctataaaag tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt
     8041 tttcaatttt atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg
     8101 ttatacttta gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat
     8161 cgcaccaaca caactgaata acttttactt tagctacttt agataggttt gtattcatca
     8221 tgatggatac aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg
     8281 cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa
     8341 gtatcaagca ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt
     8401 gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata
     8461 atcatcggat taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat
     8521 tccgcagagg catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac
     8581 tcgttagatg ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa
     8641 ggcgataatc taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt
     8701 atgagtgaga aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca
     8761 gcacaatctt tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac
     8821 ttggaacaag cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg
     8881 acagatatgg tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc
     8941 aaaacaatcg gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg
     9001 gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca
     9061 aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct
     9121 actgataaca cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa
     9181 acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga
     9241 atcgactacg aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc
     9301 aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa
     9361 gacatggcca aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca
     9421 ggaggattct catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct
     9481 tatgggccct acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact
     9541 tgactgctga aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg
     9601 gctatcatcc attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg
     9661 tttccggact gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca
     9721 tgactggttt atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg
     9781 gtcagccaac gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac
     9841 tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta
     9901 cagaaacagg acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg
     9961 ggatcaacac agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc
    10021 tttctggctc cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa
    10081 cgatcgtaat tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac
    10141 tatttctccg tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt
    10201 tagtcttcga actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag
    10261 tgatctgtct ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag
    10321 gcggtagtgt caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa
    10381 aggaacgatc aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga
    10441 cttcgtgacg atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc
    10501 cggtacattt tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa
    10561 atctgttgtc gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac
    10621 agccgttcgt ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg
    10681 cggcttcttc cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg
    10741 gctaaatttg gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca
    10801 acgtcaagct attacgttat tgatggccac attacagcgt ccagaactga tcttattaga
    10861 tgaacacact gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa
    10921 gctgatcact gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat
    10981 ccgctatggg agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca
    11041 agaggaaaaa cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg
    11101 cacggcatta aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa
    11161 aaaatgaacg cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat
    11221 caaccgttgt ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg
    11281 aaaggagctt gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc
    11341 agattttctt tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg
    11401 gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta
    11461 tcgttacctg ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt
    11521 tattcctgat ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa
    11581 actggcagca gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca
    11641 agctcgtgga cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag
    11701 tctcttgctc catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc
    11761 cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt
    11821 caatgatatc tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga
    11881 tatggaaagc ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc
    11941 tcaaaatcat tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca
    12001 agctagtgtc acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct
    12061 ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac
    12121 cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca
    12181 tggcgaactg atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag
    12241 tctcacatct atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata
    12301 cagtgtcctt ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt
    12361 tctctatcct ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt
    12421 aaatgaatca atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag
    12481 gccgcttcct ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac
    12541 ttgcgactta ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac
    12601 aaacgtctcg catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg
    12661 agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg
    12721 gaaggttcca gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct
    12781 agcagatctt ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa
    12841 tcttgttcgt tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa
    12901 attggagaag ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa
    12961 gccagccaag tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc
    13021 tcctcaatag agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc
    13081 tcgcatagca tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt
    13141 tttcctaatt ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta
    13201 acagtcacca tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat
    13261 tgaacaagaa gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt
    13321 caaacgaaca gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa
    13381 ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat
    13441 atcttttcgt atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat
    13501 atttataatg atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta
    13561 tttgctaaaa ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa
    13621 cttgatgcaa aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata
    13681 aattatatac aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca
    13741 agaatgaata aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct
    13801 acagtcaatg gatccaggct tgatcactta actccagatg atttttataa agatcgtgct
    13861 gggcatgaat atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat
    13921 cttgcaacga tcactccagc taaaacatat actgctgcag attttccaga catgaacaat
    13981 cggacaatcg atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca
    14041 gaagtgttaa cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta
    14101 tttaatgtca tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat
    14161 cgttcaaatc atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat
    14221 gagatgactg atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct
    14281 actatccgca ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat
    14341 gcagctacca atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg
    14401 acagacacaa gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact
    14461 tcaagcgaca gtacagatac tacctctacc acaagtgata gcacggatac atcagcttca
    14521 agcgacagta cagatactac ctctaccaca agtgatagca cggacacatc agcttcaagt
    14581 gacagtacag atactacctc taccacaagt gacagcacgg acacgtcagc ttcaagcgac
    14641 agtacagata ctacctctac cacaagtgat agcacggaca catcagcttc aagtgacagt
    14701 acagatacta cctctaccac aagtgatagc acggacacgt cagcttcaag cgacagtacg
    14761 gatacatcta ccacaacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
    14821 actacctcta ccacaagtga tagctcagat tcatctacca ctacaagtga tagcacggac
    14881 acgtcagctt caagtgacag tacagataca tcctctacca caagtgatag ctcagattca
    14941 tcaacttcaa gcgatagtac agacacgtcg tctaccacaa gcgatacacg tcatgcaagt
    15001 gtctccttct caaaaacaag ttcggataac aggacgaact ttaacttaac taataaaaca
    15061 agcaatgaca atggaaacaa atcaaaaaga gcacttccta aaactggaag tcaatcgaat
    15121 aattggataa cactcgcagg cgttatatta cttgtaatcg gtttaagaat cacttttatg
    15181 agttataaga aatcgaaaag gtaaaaaaat attcaagata aaaaagagca acgtcgctaa
    15241 cggacgttgc tctttatttt tgctttttat cataaataca agaactacaa ttcaatttgt
    15301 caaaaaacac gtagatttcc ttctgagaaa tttatttacg tatcgaacct ttccctattc
    15361 agtctttgcg atccaaaatc agttgggtaa gggaaaagag attctctttt gttcgaagag
    15421 aggtggcagg taatttcttt atttccttca aagccttttc tgtgtaagag gccgctaact
    15481 tcctagcttc ttccacgcct cctgtatgtt caacgatttt gcgtacggcg tcgacatctt
    15541 cttctgtcat tgccgctttc ttctctaaat aaggtagtag ctttggtttg tttttttgca
    15601 aagagtagat caacggtaaa gaatagaccc cttgacgcat gtcttctagt acaggtttgc
    15661 cgatttcctg gctctctttt gtataatcta aaatatcatc gataatctga aaggccatcc
    15721 cgatagaaag tccgatatct cctgcttttt ttgcgaagcg ctcgcttgtt ccactttcaa
    15781 aagcgccaac agaacaactt aattggaaca gttcagctgt cttacctgaa atattttcca
    15841 aatattcttt tacagaaagt tcataattat agcgatcatc catctgccct aattcgccat
    15901 tcaatacttt ttccatactg cgagaattca attggagact tttcaaggaa gtggagtaat
    15961 cagacaatag tttaaaacag cagacaaata aatagtctcc tgcatagaca gaaacgacat
    16021 taccaaactg actacggata ttaggctgac cacgtcgcag atccgcttca tcaacgatat
    16081 catcatggat cagtgtggcc gtatggagca tttcgataga agctgccaaa gcgatggctt
    16141 tgtctcgatc ttgctctggt ccaaattgtg agaacaatag ctgataggca ggacgcaaca
    16201 gctttccgcc ggcatggatc gtttctttga tggcttgttc gacaggtttg tttttcaggc
    16261 ggacagtttc ttccatccgt tttagtgttt ctgtcaattc tttacttaaa gcaggatagt
    16321 tttcccacat tggatgtagc ttcataaaaa aactccttgt gtgacgatta ttttttggta
    16381 aacattgggc tccatttttg tttggttccc tgctcataat ctttttggaa tcgttgaagt
    16441 gtctgcacat gtcgtaaaag ataattcgct gcgatcccga ttgcaatccc tgataaaatc
    16501 cctaaaactg aaaggactgg caagtacaac atgactgtcc atgattgtgc aatgaaagaa
    16561 gccatcaaaa gctgtccgac attatgcatg aaaccgccgg ccgcactaat tccgatgata
    16621 ctgacacgtt ttggtcctaa ttgcttgacc aataacattc caaaataact aagcaaagca
    16681 ccactcatac tataaaggaa tgtggaaatc gttccgccta gcaaagtagt caggaatagt
    16741 cgtagccaaa gtagtaagaa gctgtcttgt tttttcatgg taaaaatggc aacgatcgtg
    16801 attagattgg ctaagcctag ttttgcacca ggcgcaaaag aaaaaggata agggatcata
    16861 ttttccaata aaccaatgat cactccttgt gcaacaagta gcgaaatata aatcattttt
    16921 tgtaatttac tcatggtgtg tgaaggtcta ataaatcaga ctgtcttcgt cacctccatc
    16981 tgttgaacgg acttcgatca ctaatttatg tggcaaacag acgatggttt cgccattttt
    17041 cgttgcccag cctcgacgta cgcagatctg gtcatcgcaa tctgcttctt ttatccgtac
    17101 tcgatcgcca tctatctcga tcaagttata atttccgtgt tcatctttgt aagtatatgt
    17161 gttgctctct tctccagcca tcaaagggaa tgttttgatt tctgtgccat ccacacgcag
    17221 taccgcttct ttttctgctg ttgcggtgtt ttgctgaaga ccaaaaatca tgactggtaa
    17281 aaaagaaaga acagtcaaaa gcaaaataat aaaaacatcc caaggtttga gcagactttt
    17341 ttcagcaaat tctttaaggc tcatttttgt tctccttttt ccagattaac tcacccaatt
    17401 ttagcataaa aacaagaggt tgtgacacct gtgtcttgct ttgagtaata agaacgaaga
    17461 atagaaaaca gctttccatg ttttctaatt ctttgggctt attaccgaga agcagcaggt
    17521 ggaacaccgt ttataacaag aggttgtgac agaagtgggc aacttcaaga aataagaagg
    17581 aatttacgaa aattactttt caagtttttg tgaaatttcg gcttattttt cgaagaagct
    17641 acttctggaa caccgtttat aacaagaggt tgtaacagag gttgtgacac ctgtgtcttg
    17701 ctcatggagg cttatctcgg gcatcttatt cctttaattt tacataaaaa ggaggctgtg
    17761 acggtttgtc tcagcccctt tctattgaaa tcttgcattc aaataacgtt ctttatctat
    17821 ttatttcttg ccgtactggg aacttggtct accgtaccac caattaccta gtgtacgttg
    17881 tagccatgga attacccagc gatccaaccc aatcgcacgt ccggaaccgt tcatcaatgc
    17941 gaaagcaaca aaaatgaacc aaatgtttac ccagtaaaac atgcctgata agcagaacgt
    18001 gatcaccaat acaatcgttg tcgcactgca tagccatgtg aataatccag caatcaatgc
    18061 taatgccaag gcaatttcaa agaataccat gaatttttgg aagaacattg ctacatcatt
    18121 gtttggcatg atcacttgca tgatctttgt gaaccagtct ggtgacttct caatcacagc
    18181 cattggttct tcaccgtatg cataactcaa accaaaatgt gcagcttgag tcgctgcttc
    18241 tccggcactt gcccctgctc cgctggcagc tgtggttgca tcaccagcag cagaagcacc
    18301 actggcaaca tctgttacat tgtattccca tgtccaaggg aacacactgt ttccagtgaa
    18361 ccatgatcct tcaccaaacc aagtacttgg tttgaacact tcaccattcc caatgatttt
    18421 tttggaagct tcaacgaacc aaactaaacc atagaaaata cgaagtggca cactccataa
    18481 gacattgccg taacgagaag aatgtcctcg agtcacgttg cgatcatctt tgatatggaa
    18541 aaactcatgc attaaatatt ggaacatata ataaccagaa cgaatatcaa agaagtattt
    18601 caaattcacg atatgtttca ttacgatagc taaaaatccg cttaaatgga tcttatcaaa
    18661 taggttagct accccccatt tggcaccaat agaaaccata aatccttgat agtttccttt
    18721 aaatttatgc ttttctccgc ctttgatcga agccacgata ttcgctgcgg ctgtgtgtcc
    18781 tgtttgttca gcagcttgaa cgatctgcgg tgtcggtgtt cctgggaatt cttcataaaa
    18841 gactaaatca ccaatgatat agatgttttt atcttcgtag ccttttgcct gcatatattc
    18901 attggcgatc aaccgctgtc cgcgtgctga ttctaatcca aagtcagcag cgtcagaagt
    18961 tcctttgaca ccagctgtcc aaatcagtgt gtgagtcgga atgttgcgtc cgtctttcaa
    19021 tttgatatga tcagcagcca cttctacgat tggtgcattc aataataatt ccacgttgtg
    19081 cttacgcaaa tagcgttcag cttttgcagc atcatttcga tccaacatat tcaagatcgt
    19141 cggcattgcc tctacgacca tcagtgtgat ttcgtttgga tctaatttat aatctttggc
    19201 taggcgattt ttccagtcga tcagttcccc aatcatttca ataccggtaa atccagaacc
    19261 acagacaacg aaagtcagca tggctttacg cagttcatca tctggttcca atgcagcttt
    19321 tgctactgtc ttctcaatat gttcccgaat ccttacagaa tcttcaaatg accaaagggt
    19381 gaatccgtgt gctttgacac caggtgtacc aaagtcattc ggctctccgc ccattccaag
    19441 gatcagctga tcaaaaggat aggaacctaa tttggtcttg acgatttttc gttctttatc
    19501 gataccgatt acagtgtctg tgacaagttg tacattcttt tgacgagcaa acaaatgctg
    19561 cagatcgtat tggattgctt ctggttctac acgtccgcca gctacttcat gcaactcggt
    19621 catcatagta tggtacgaat gacgatcgat cagagtaatt gctacatctt cttttttcag
    19681 tttctttgcc aagtattttg tagctgaaac accggcatat cctgctccta caacgacaat
    19741 attttgcttt gccattgatc atccgctcct tatagactat ctatataatg aatatatgat
    19801 aattctttta gtttgatact tattacacaa tctccatata ttatatagat gttctgttct
    19861 tttgtctaac tccttttgga tttttttcaa attttaaaat aaagcgtttt ttcacgaaat
    19921 ttgctgaaag aaaacgtttg aaatattaca tgggttattt atcaaaaata gttgacttat
    19981 aggctaaaaa gattagaatg tataatgaat cacaaagaaa aattgtggac tagaaatgga
    20041 atggtggggt attaaaatga agaaaaaacg ttttgtaact ggatttgccg tattagcatt
    20101 ttcagctcta gtgttaggcg cttgcggcgc agatgacaac ggaagctcaa actcttcttc
    20161 tgaatcatct acagcacaat cttcgactgc aaaaagcaca acagaatcat ctgcaaaagt
    20221 tgtagctggc ggagatttgc aagacggcac atataaatta gaagaaaaaa attattcaaa
    20281 tggttaccgt gcagtctttg aaatggttgt aaaagacggc aaaatcactg aatcaaaata
    20341 tgataacgtc aatgaaaacg gcgaatcaaa aacaaaagat gcagaataca acaaaaacat
    20401 ggaagcaaaa tctggcacaa acccagaaaa atacattcct gagttgaatg accaattctt
    20461 aaaagcacaa agcgcaagcg gtgtggaagt cgtaactgga gctactcact catctgaaag
    20521 cttccaaaac tacgcacaac aattgatcca agctgctcaa gctggtaaca cagatacaat
    20581 cgaaatcgac aatggtgctg atttgaaaga cggcacttac aagcttgaag aaaagaacta
    20641 ctctaacggc taccgtgtgc aattcgaaat gacagttgct ggcggcaaag taactgaatc
    20701 aaactttgat tatatcgaca aagacggtaa atcaaaacaa gatgatacag aatataatga
    20761 aaatatgaaa gcaaaaagcg gtacagaacc aaaaacttac atccctactc tgaacgacga
    20821 attcgtcaaa gcaatgggcg aagaagatgg ctcacctgct aatgtagaag ttgttacagg
    20881 tgctacacac agctcacatt cattcatcat gtacgcacaa caattagtga atgcagctga
    20941 aaaaggcgac actcaaacaa tcgaagtaga taatatcgta acagaaaaat aatcacataa
    21001 tcatactaat aaaagttgga gggtgggacg atagccagat taggcagtcg tctcaccttt
    21061 caaaatgaag agaggaaaaa gatgcggaaa aaactactat tttccttaat cacactgggc
    21121 atctctttta tggtgctaac tggctgcagc aactcagcaa ataaaacaac cactgaaagt
    21181 tcagcagcta ccaagatatt gaaagatcca tattctgatg aacagttctt gttagggact
    21241 tacgttcgta tccgtgtgta tgatgaaggc aaagaatcag cattgaaacc tgccttcgat
    21301 cgagtaaaag aacttggtga taaaatcacg atcaatcaaa aaggttctga gatcgatgaa
    21361 gtgaatgagc aagctgggat caaaccagta aaagtttctg atgacgtcta cacgttagtc
    21421 aagcgggcct acgaatatag ccaagattct caaggcggat tcgatatggc gattggtgca
    21481 atcacacaat tatggcgaat cggttttgat gacgccagaa aaccttccca agaagaaatc
    21541 gatcaggcat tgaaattagt ggattatcat aagatcgaac taaatgacaa agaaaaaact
    21601 gtttatctaa aagaaaaagg aatgatcatc gatttaggtg caatcgccaa aggttacatt
    21661 acggacgaag tagtgaaagt cttgaaaaaa caaggagtga ccactgccat cgttgacttg
    21721 ggcggtaatg tctatgtctt aggccacagc ccaagaggcg aaaaccaaga ctggacagtc
    21781 ggcatccaag atccgaacat ggctcgcgga tctgttttag gttctattaa agaacggaac
    21841 aaaacattag ttacctctgg aatttacgag cgttatttag aagtagatgg caaaaaatac
    21901 catcatttgt ttgatccaaa aacgggttat ccgttcgaca atgatatcgc aagtgtcacg
    21961 atcataacag ataaatcaat tgatggtgac gggttgtcta cagcagtctt ttcaatgggt
    22021 gtgaaaaaag gactggaata cgtcgaaagc gaattgaata acgggacgga agcgatcttc
    22081 gtgacaaaag acgataaagt atatgtgacc gatccaatca aagacacctt caaattatcg
    22141 gaggactccc attacacgat gggtgaccgt tctgaactaa aataaggagt acgaaccatg
    22201 acactaaaaa tctttttaga agtagttgaa atacgaacaa aggtggctag tgtatttcct
    22261 tttgcggtag gggtattatt ctctatcgct ttttttcatg agatacattg gctaaacact
    22321 tttttatttt ttattggaat gctagttttt gatttagcga ctaccgctat caataa
//
LOCUS       DACDKJ010000046        22031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDKJ010000046 DACDKJ010000000
VERSION     DACDKJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.046"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            367..555
                     /locus_tag="IV328_001891"
     CDS             367..555
                     /locus_tag="IV328_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938028.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="IV328_001892"
     CDS             545..805
                     /locus_tag="IV328_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938029.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="IV328_001893"
     CDS             846..1073
                     /locus_tag="IV328_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938030.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="IV328_001894"
     CDS             1138..1710
                     /locus_tag="IV328_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938031.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="IV328_001895"
     CDS             1711..2022
                     /locus_tag="IV328_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8938032.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="IV328_001896"
     CDS             2013..2273
                     /locus_tag="IV328_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938033.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="IV328_001897"
     CDS             2434..2793
                     /locus_tag="IV328_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938034.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="IV328_001898"
     CDS             2790..4433
                     /locus_tag="IV328_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8938035.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="IV328_001899"
     CDS             4468..5550
                     /locus_tag="IV328_001899"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938036.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="IV328_001900"
     CDS             5594..6766
                     /locus_tag="IV328_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8938037.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="IV328_001901"
     CDS             6753..7457
                     /locus_tag="IV328_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8938038.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="IV328_001902"
     CDS             7462..8622
                     /locus_tag="IV328_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8938039.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="IV328_001903"
     CDS             8698..8976
                     /locus_tag="IV328_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8938040.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="IV328_001904"
     CDS             8957..9328
                     /locus_tag="IV328_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8938041.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="IV328_001905"
     CDS             9344..9748
                     /locus_tag="IV328_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938042.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="IV328_001906"
     CDS             9745..10155
                     /locus_tag="IV328_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938043.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="IV328_001907"
     CDS             10216..10803
                     /locus_tag="IV328_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8938044.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="IV328_001908"
     CDS             10876..11343
                     /locus_tag="IV328_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP8938045.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="IV328_001909"
     CDS             11389..11697
                     /locus_tag="IV328_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938046.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="IV328_001910"
     CDS             11892..18626
                     /locus_tag="IV328_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8938047.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="IV328_001911"
     CDS             18630..19502
                     /locus_tag="IV328_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938048.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20126
                     /locus_tag="IV328_001912"
                     /pseudo
     CDS             19499..20126
                     /locus_tag="IV328_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20087..20548
                     /locus_tag="IV328_001913"
     CDS             20087..20548
                     /locus_tag="IV328_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938049.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="IV328_001914"
     CDS             20552..21376
                     /locus_tag="IV328_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938050.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..21939
                     /locus_tag="IV328_001915"
                     /pseudo
     CDS             21394..21939
                     /locus_tag="IV328_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         7878 a         3297 c         4670 g         6186 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacttataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DACDKJ010000047        21911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDKJ010000047 DACDKJ010000000
VERSION     DACDKJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.047"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IV328_001916"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IV328_001916"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8938051.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IV328_001917"
     CDS             complement(1172..1867)
                     /locus_tag="IV328_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8938052.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IV328_001918"
     CDS             complement(1880..2596)
                     /locus_tag="IV328_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8938053.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IV328_001919"
     CDS             2926..3534
                     /locus_tag="IV328_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8938054.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IV328_001920"
     CDS             3859..4182
                     /locus_tag="IV328_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8938055.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IV328_001921"
     CDS             4364..5734
                     /locus_tag="IV328_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8938056.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IV328_001922"
     CDS             complement(5864..7165)
                     /locus_tag="IV328_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8938057.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IV328_001923"
     CDS             complement(7519..9510)
                     /locus_tag="IV328_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8938058.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IV328_001924"
     CDS             complement(9654..9920)
                     /locus_tag="IV328_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8938059.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IV328_001925"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IV328_001925"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8938060.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IV328_001926"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IV328_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8938061.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IV328_001927"
     CDS             13264..13827
                     /locus_tag="IV328_001927"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8938062.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IV328_001928"
     CDS             complement(14080..14601)
                     /locus_tag="IV328_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8938063.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IV328_001929"
     CDS             complement(14604..15446)
                     /locus_tag="IV328_001929"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8938064.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IV328_001930"
     CDS             complement(15439..16164)
                     /locus_tag="IV328_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8938065.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IV328_001931"
     CDS             16340..16636
                     /locus_tag="IV328_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938066.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IV328_001932"
     CDS             17204..18796
                     /locus_tag="IV328_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8938067.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IV328_001933"
     CDS             18809..19549
                     /locus_tag="IV328_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8938068.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IV328_001934"
     CDS             19717..21066
                     /locus_tag="IV328_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8938069.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IV328_001935"
     CDS             complement(21194..21784)
                     /locus_tag="IV328_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8938070.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6294 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa a
//
LOCUS       DACDKJ010000048        21666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDKJ010000048 DACDKJ010000000
VERSION     DACDKJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.048"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(205..1104)
                     /locus_tag="IV328_001936"
     CDS             complement(205..1104)
                     /locus_tag="IV328_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8938071.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="IV328_001937"
     CDS             complement(1114..1470)
                     /locus_tag="IV328_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8938072.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="IV328_001938"
     CDS             1825..2121
                     /locus_tag="IV328_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8938073.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="IV328_001939"
     CDS             complement(2765..3718)
                     /locus_tag="IV328_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8938074.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="IV328_001940"
     CDS             complement(3741..4496)
                     /locus_tag="IV328_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8938075.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="IV328_001941"
     CDS             complement(4493..5452)
                     /locus_tag="IV328_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8938076.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="IV328_001942"
     CDS             complement(5445..6395)
                     /locus_tag="IV328_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8938077.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="IV328_001943"
     CDS             6545..6997
                     /locus_tag="IV328_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8938078.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="IV328_001944"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="IV328_001944"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8938079.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="IV328_001945"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="IV328_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8938080.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="IV328_001946"
     CDS             complement(10549..11358)
                     /locus_tag="IV328_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8938081.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="IV328_001947"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="IV328_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8938082.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /locus_tag="IV328_001948"
     CDS             complement(14968..15654)
                     /locus_tag="IV328_001948"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8938083.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="IV328_001949"
     CDS             complement(16002..17783)
                     /locus_tag="IV328_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8938084.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="IV328_001950"
     CDS             complement(17809..18723)
                     /locus_tag="IV328_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8938085.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /locus_tag="IV328_001951"
     CDS             complement(18750..19712)
                     /locus_tag="IV328_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8938086.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="IV328_001952"
     CDS             complement(19712..20656)
                     /locus_tag="IV328_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938087.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="IV328_001953"
     CDS             complement(20657..21661)
                     /locus_tag="IV328_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938088.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6048 a         4625 c         3839 g         7154 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaa
//
LOCUS       DACDKJ010000049        20873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDKJ010000049 DACDKJ010000000
VERSION     DACDKJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.049"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IV328_001954"
     CDS             99..293
                     /locus_tag="IV328_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938089.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="IV328_001955"
     CDS             562..861
                     /locus_tag="IV328_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938090.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="IV328_001956"
     CDS             complement(958..1392)
                     /locus_tag="IV328_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8938091.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="IV328_001957"
     CDS             complement(1624..2334)
                     /locus_tag="IV328_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8938092.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="IV328_001958"
     CDS             complement(2324..3184)
                     /locus_tag="IV328_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8938093.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="IV328_001959"
     CDS             complement(3189..3827)
                     /locus_tag="IV328_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8938094.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="IV328_001960"
     CDS             complement(3842..4141)
                     /locus_tag="IV328_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8938095.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="IV328_001961"
     CDS             complement(4184..5668)
                     /locus_tag="IV328_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8938096.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="IV328_001962"
     CDS             complement(5688..6149)
                     /locus_tag="IV328_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8938097.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IV328_001963"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IV328_001963"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8938098.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="IV328_001964"
     CDS             7452..8222
                     /locus_tag="IV328_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8938099.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="IV328_001965"
     CDS             complement(8495..9322)
                     /locus_tag="IV328_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8938100.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="IV328_001966"
     CDS             9890..11125
                     /locus_tag="IV328_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8938101.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="IV328_001967"
     CDS             complement(12124..12384)
                     /locus_tag="IV328_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8938102.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="IV328_001968"
     CDS             complement(12411..13712)
                     /locus_tag="IV328_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8938103.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="IV328_001969"
     CDS             complement(14004..14462)
                     /locus_tag="IV328_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8938104.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15800)
                     /locus_tag="IV328_001970"
     CDS             complement(14475..15800)
                     /locus_tag="IV328_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8938105.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17229)
                     /locus_tag="IV328_001971"
     CDS             complement(15817..17229)
                     /locus_tag="IV328_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8938106.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="IV328_001972"
     CDS             complement(17246..18010)
                     /locus_tag="IV328_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8938107.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="IV328_001973"
     CDS             complement(18114..18989)
                     /locus_tag="IV328_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8938108.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="IV328_001974"
     tRNA            complement(19244..19328)
                     /locus_tag="IV328_001974"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="IV328_001975"
     tRNA            complement(19346..19417)
                     /locus_tag="IV328_001975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="IV328_001976"
     tRNA            complement(19427..19499)
                     /locus_tag="IV328_001976"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="IV328_001977"
     tRNA            complement(19561..19645)
                     /locus_tag="IV328_001977"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="IV328_001978"
     tRNA            complement(19666..19736)
                     /locus_tag="IV328_001978"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
     gene            complement(19782..19853)
                     /locus_tag="IV328_001979"
     tRNA            complement(19782..19853)
                     /locus_tag="IV328_001979"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19819..19821),aa:Gln,seq:ttg)
     gene            complement(19865..19937)
                     /locus_tag="IV328_001980"
     tRNA            complement(19865..19937)
                     /locus_tag="IV328_001980"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19902..19904),aa:His,seq:gtg)
     gene            complement(19940..20013)
                     /locus_tag="IV328_001981"
     tRNA            complement(19940..20013)
                     /locus_tag="IV328_001981"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19979..19981),aa:Trp,seq:cca)
     gene            complement(20020..20100)
                     /locus_tag="IV328_001982"
     tRNA            complement(20020..20100)
                     /locus_tag="IV328_001982"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20064..20066),aa:Tyr,seq:gta)
     gene            complement(20102..20177)
                     /locus_tag="IV328_001983"
     tRNA            complement(20102..20177)
                     /locus_tag="IV328_001983"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20142..20144),aa:Phe,seq:gaa)
     gene            complement(20213..20285)
                     /locus_tag="IV328_001984"
     tRNA            complement(20213..20285)
                     /locus_tag="IV328_001984"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20250..20252),aa:Asp,seq:gtc)
     gene            complement(20295..20367)
                     /locus_tag="IV328_001985"
     tRNA            complement(20295..20367)
                     /locus_tag="IV328_001985"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20332..20334),aa:Val,seq:tac)
     gene            complement(20383..20454)
                     /locus_tag="IV328_001986"
     tRNA            complement(20383..20454)
                     /locus_tag="IV328_001986"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20419..20421),aa:Glu,seq:ttc)
     gene            complement(20469..20558)
                     /locus_tag="IV328_001987"
     tRNA            complement(20469..20558)
                     /locus_tag="IV328_001987"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20522..20524),aa:Ser,seq:gga)
     gene            complement(20598..20671)
                     /locus_tag="IV328_001988"
     tRNA            complement(20598..20671)
                     /locus_tag="IV328_001988"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20635..20637),aa:Asn,seq:gtt)
     gene            complement(20681..20796)
                     /gene="rrf"
                     /locus_tag="IV328_001989"
     rRNA            complement(20681..20796)
                     /gene="rrf"
                     /locus_tag="IV328_001989"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6573 a         4383 c         3325 g         6592 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatagaa actggggtag ctggattcga
    19801 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa
    19861 aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac
    19921 cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg
    19981 gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac
    20041 cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc
    20101 atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc
    20161 aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg
    20221 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
    20281 gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag
    20341 atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg
    20401 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
    20461 atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat
    20521 ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact
    20581 ctttctcaaa atgagaactc cggcagtagg actcgaacct acgacatcat gattaacagt
    20641 catgcgctac taccaactga gctatgccgg aataacatca gcgtggcgac gtcctactct
    20701 cacaaggggc aacccctcac tacaatcggc gctaagaagc ttaacttctg tgttcggcat
    20761 ggttacaggt gtatccttct cgctatcgcc accacactgt ggtgttatct tttattgagt
    20821 aaattttgtt cactcaaaac tggatttgaa gtcacataag tttttttccg agt
//
LOCUS       DACDKJ010000050        20489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDKJ010000050 DACDKJ010000000
VERSION     DACDKJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.050"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="IV328_001990"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="IV328_001990"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8938109.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="IV328_001991"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="IV328_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8938110.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLVQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="IV328_001992"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="IV328_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8938111.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="IV328_001993"
     CDS             2857..3732
                     /locus_tag="IV328_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8938112.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IV328_001994"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IV328_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8938113.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="IV328_001995"
     CDS             4629..5063
                     /locus_tag="IV328_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8938114.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="IV328_001996"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="IV328_001996"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8938115.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="IV328_001997"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="IV328_001997"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8938116.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="IV328_001998"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="IV328_001998"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8938117.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="IV328_001999"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="IV328_001999"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8938118.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="IV328_002000"
     CDS             13334..14260
                     /locus_tag="IV328_002000"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8938119.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="IV328_002001"
     CDS             14442..15335
                     /locus_tag="IV328_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8938120.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="IV328_002002"
     CDS             15469..15594
                     /locus_tag="IV328_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8938121.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="IV328_002003"
     CDS             complement(15728..16360)
                     /locus_tag="IV328_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938122.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="IV328_002004"
     CDS             complement(16508..17050)
                     /locus_tag="IV328_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8938123.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="IV328_002005"
     CDS             17185..17574
                     /locus_tag="IV328_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938124.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="IV328_002006"
     CDS             17578..18342
                     /locus_tag="IV328_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8938125.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="IV328_002007"
     CDS             18471..19418
                     /locus_tag="IV328_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8938126.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="IV328_002008"
     CDS             19548..20288
                     /locus_tag="IV328_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938127.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5499 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggtgcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtttatt
//
LOCUS       DACDKJ010000051        20412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDKJ010000051 DACDKJ010000000
VERSION     DACDKJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.051"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..1091
                     /locus_tag="IV328_002009"
     CDS             12..1091
                     /locus_tag="IV328_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8938128.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1288..1989)
                     /locus_tag="IV328_002010"
                     /pseudo
     CDS             complement(1288..1989)
                     /locus_tag="IV328_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2003..2927)
                     /locus_tag="IV328_002011"
                     /pseudo
     CDS             complement(2003..2927)
                     /locus_tag="IV328_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3044..4123
                     /locus_tag="IV328_002012"
     CDS             3044..4123
                     /locus_tag="IV328_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8938129.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4155..4748
                     /locus_tag="IV328_002013"
     CDS             4155..4748
                     /locus_tag="IV328_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8938130.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4858..5085)
                     /locus_tag="IV328_002014"
     CDS             complement(4858..5085)
                     /locus_tag="IV328_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8938131.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5188..7776)
                     /locus_tag="IV328_002015"
     CDS             complement(5188..7776)
                     /locus_tag="IV328_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8938132.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7796..8455)
                     /locus_tag="IV328_002016"
     CDS             complement(7796..8455)
                     /locus_tag="IV328_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8938133.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8504..9007)
                     /gene="trmL"
                     /locus_tag="IV328_002017"
     CDS             complement(8504..9007)
                     /gene="trmL"
                     /locus_tag="IV328_002017"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8938134.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9228..10094)
                     /locus_tag="IV328_002018"
     CDS             complement(9228..10094)
                     /locus_tag="IV328_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8938135.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10203..10844
                     /locus_tag="IV328_002019"
     CDS             10203..10844
                     /locus_tag="IV328_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8938136.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10906..12267)
                     /gene="mgtE"
                     /locus_tag="IV328_002020"
     CDS             complement(10906..12267)
                     /gene="mgtE"
                     /locus_tag="IV328_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8938137.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12285..13184)
                     /locus_tag="IV328_002021"
     CDS             complement(12285..13184)
                     /locus_tag="IV328_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8938138.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13186..13983)
                     /locus_tag="IV328_002022"
     CDS             complement(13186..13983)
                     /locus_tag="IV328_002022"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8938139.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13961..14665)
                     /locus_tag="IV328_002023"
     CDS             complement(13961..14665)
                     /locus_tag="IV328_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8938140.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14796..15371
                     /locus_tag="IV328_002024"
     CDS             14796..15371
                     /locus_tag="IV328_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8938141.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15489..16166
                     /locus_tag="IV328_002025"
     CDS             15489..16166
                     /locus_tag="IV328_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8938142.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16245..18053)
                     /gene="pepF"
                     /locus_tag="IV328_002026"
     CDS             complement(16245..18053)
                     /gene="pepF"
                     /locus_tag="IV328_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8938143.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18229..19227)
                     /locus_tag="IV328_002027"
     CDS             complement(18229..19227)
                     /locus_tag="IV328_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8938144.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19453..20106)
                     /locus_tag="IV328_002028"
     CDS             complement(19453..20106)
                     /locus_tag="IV328_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8938145.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5907 a         4440 c         3638 g         6427 t
ORIGIN      
        1 gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga aaacatcttt
       61 cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc aaccgtcagg
      121 ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat cgcggaacca
      181 tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac tattctgttt
      241 atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt ggcagacgcc
      301 ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg ttaaatgaaa
      361 aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc gattgtgcca
      421 tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg cgaacaaaga
      481 acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca ggacaccgtc
      541 ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt gaaacacggg
      601 aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag tcggatgccg
      661 tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag ttgaacggga
      721 aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct ggcgctaact
      781 tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct gggctacacg
      841 atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct tgggagcgtg
      901 ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga agcccgatta
      961 gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat tacccgcgga
     1021 aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag caagaacggt
     1081 tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg cttcatgtct
     1141 ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt cattatcaag
     1201 gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt tacctgtagt
     1261 ggctaactta aacttgaaat ttacggacgt aaagcttgct gccaaattag cgattgaaac
     1321 taacgattgc tttctaggaa tcaaggacaa gcgaatacca tttgacgatt gatttgcttg
     1381 ctgatcctgt acattgcgga ccccccacat cgtcccgtag agaatcgcca ttccaatcag
     1441 agtgaagaaa taaaaacttt tgaaactcat attacgacct gcatgaactt cttgaaaaga
     1501 tacttcttgt tcaaaaacag tcatgatggg cgcgagatca acgttctcca cttctgccac
     1561 tttactcgaa attataccaa tacgctgtaa aaattgatcc atgacgtttt tcatcaacgt
     1621 ctgactcatt cctgcttgac ccacatggag cgaaacagat gaaccatcaa tgagataaaa
     1681 accagccaaa tcatctgcat ttagcttttc attcgcttct acttccgata gttcctgtac
     1741 ttcaaataat ggtttatcat tgacttcgac ttgttctaag actgtggtaa aattttccga
     1801 aagcgctgtt tgatccgctt tgattacaat tccagtatta atcgtttcga gattgccacc
     1861 ttgatcaatc tcgccaaagg ccgccataaa catcaatcct aaaagaattg gaaaggccaa
     1921 tgtccaaaac aacatggatt tatttcgcag cagtattttt gtgcgatatt tataaaaatg
     1981 ccaaaacatt atttcccctc ctctagtctc tcaaatcctt gccagtaatc tctaaaaaga
     2041 catcattcaa ggacgcttct ttggttgcta tttttccgta actgatcttt tcttcttgta
     2101 agattgtaag aatcggcaac aatactgtat ttagttcagt agttgttact tggagctgac
     2161 cttttttata agcgattcca tcagaagcta ttgttcgctg acaacacgtt ttctgggagt
     2221 aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag agattctttg
     2281 gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca aagaatttct
     2341 acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt cagctccttg
     2401 atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg ctcatctaaa
     2461 aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg tttcaagcca
     2521 ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa ttgaatcacc
     2581 tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca aaaataatcg
     2641 atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc
     2701 attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt aatggttcct
     2761 ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc actgccgttt
     2821 ggtcctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg attcaatgct
     2881 acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat gaaaaactcc
     2941 ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact agtgacgatt
     3001 ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata aatctcggct
     3061 actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa gtcaaggatt
     3121 cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg tcagttattt
     3181 actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat gtgccttttt
     3241 acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg aagaaagcca
     3301 ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta atcatccccc
     3361 catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac gaaaaaaaga
     3421 tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc attgggaaaa
     3481 acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa cacaattagc
     3541 cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac atccatgaca atgtcggtca
     3601 tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa
     3661 actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg atcggattcg
     3721 tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct tagagtttct
     3781 gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag gattgaatga
     3841 aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca gtatcaaaca
     3901 tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt atcgcttgca
     3961 aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg gtttgaaaac
     4021 gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag aaaaccagca
     4081 atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt agtcattatt
     4141 gagatgatcc atttgtgacg aaatcgttac aaaccatttt agaaagcacg aaagagatcc
     4201 gtgtgttagg cattggtcat tgtgccaagg atgcgctagt cttatacgaa acccatcgtc
     4261 ccgatgtttt gctgacggac atccgcatgc ctaaacaaac gggcatcgat gctgccaaag
     4321 aaattttggc taaatttccc aaagccatcc ttttgctttt gacgacattc aaagatgaag
     4381 aatacatccg cgaagcattt tccatcggtg tcaaagggta tttaatcaaa cagaacttgc
     4441 aagcgattat tccttctgtc aaatccgctt ataatggaca ggccgtattt ggtaatgaaa
     4501 tcatcgagac tttcactcaa atgatgaaga ataaaccgac gattgatagt catgcttatg
     4561 cttcttttag tgaacgtgag cttgcaatca tcaaagaagt agctgccggg aaaaacaata
     4621 aagaaattgc agatgccttg tacttgagtg acggcactgt tcgcaattac atcagccaat
     4681 tactagaaaa gctagattta cgggatcgca cgcaattggc tatctatttt tatcagcatt
     4741 tgcaataagt cttatctaac acaaaaaaac ctgaatgatg ctccttcgca tcattcaggt
     4801 ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca
     4861 aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg
     4921 cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg
     4981 actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga
     5041 ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc
     5101 ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc
     5161 atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg
     5221 agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt
     5281 aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa
     5341 tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc
     5401 actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc
     5461 atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag
     5521 agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc
     5581 tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc
     5641 cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg
     5701 gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca
     5761 ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa
     5821 ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat
     5881 ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt
     5941 gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg
     6001 attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat
     6061 tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat
     6121 tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg
     6181 attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat
     6241 actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat
     6301 ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc
     6361 tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt
     6421 atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac
     6481 tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag
     6541 acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg
     6601 aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag
     6661 attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa
     6721 tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc
     6781 gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc
     6841 ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa
     6901 actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat
     6961 aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg
     7021 gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg
     7081 ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc
     7141 gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag
     7201 ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact
     7261 gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc
     7321 gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat
     7381 ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc
     7441 tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc
     7501 tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct
     7561 tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat
     7621 atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact
     7681 gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac
     7741 tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata
     7801 gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt
     7861 catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca
     7921 atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag
     7981 ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgttgg gcaaccgcat
     8041 ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag
     8101 aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt
     8161 acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg
     8221 tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg
     8281 ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa
     8341 atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa
     8401 aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca
     8461 gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat
     8521 tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca
     8581 ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca ttcataggga
     8641 tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt
     8701 ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat
     8761 gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta
     8821 aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat
     8881 gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg
     8941 cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat
     9001 gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt
     9061 tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga
     9121 agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac
     9181 ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta
     9241 gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa
     9301 ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg
     9361 gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt
     9421 tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt
     9481 ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc
     9541 accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa
     9601 ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa
     9661 tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt
     9721 gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag
     9781 ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata
     9841 gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt
     9901 ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa
     9961 aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct
    10021 tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca
    10081 atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt
    10141 ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt
    10201 agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca attgctagag
    10261 gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg
    10321 gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta
    10381 ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg
    10441 atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt
    10501 caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga
    10561 tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt
    10621 ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta
    10681 ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca
    10741 ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg
    10801 taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa
    10861 acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc
    10921 cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt
    10981 gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat
    11041 taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa
    11101 ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac
    11161 agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc
    11221 tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt
    11281 accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa
    11341 gtcttcatag tgactgatta aagaagcggt cgacataccc aaaaacaaca gagtgatcag
    11401 ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac
    11461 cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc
    11521 gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc
    11581 ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac
    11641 tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag
    11701 agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc
    11761 agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac
    11821 gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag
    11881 ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt
    11941 tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc
    12001 agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc
    12061 aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct
    12121 gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa
    12181 actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct
    12241 ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt
    12301 ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta
    12361 aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc
    12421 attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact
    12481 cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt
    12541 cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc
    12601 aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct
    12661 gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc
    12721 atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaaacc agatgtatcc
    12781 cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa
    12841 gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg
    12901 cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt
    12961 tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca
    13021 tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct
    13081 tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt
    13141 acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat
    13201 aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg
    13261 ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc
    13321 aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt catcatgggc
    13381 aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc
    13441 caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc
    13501 tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc
    13561 gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt
    13621 cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa
    13681 gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc
    13741 gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac
    13801 atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac
    13861 tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc
    13921 gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt
    13981 catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag tagacatcat
    14041 ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac
    14101 tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca
    14161 tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga
    14221 aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca
    14281 gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct tttttatttg
    14341 tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa
    14401 cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt
    14461 cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga
    14521 ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact
    14581 gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta
    14641 aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt
    14701 attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga
    14761 aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat
    14821 ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat caattaacag
    14881 ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga
    14941 aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa
    15001 cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag
    15061 catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt
    15121 tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgggctgaat
    15181 tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt
    15241 ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt
    15301 taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag
    15361 ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc
    15421 tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct attttggggg
    15481 agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa
    15541 tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga
    15601 tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa
    15661 aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg
    15721 cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga
    15781 tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat
    15841 ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact
    15901 tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac
    15961 acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag
    16021 taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat
    16081 atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta
    16141 gccatttaca cttgctttct aattaaaaaa atacgtcgac tcccttacaa gagagccgac
    16201 gtattttttt cgtttctatt cgagaaaatc aaaaatagaa agccttataa acgatcgatc
    16261 aattcttcta attcatttag acgttgttca aacatcgaca ttgcatcttc aatatatgca
    16321 gcctgtgtca tatcaactcc tgcttttttc attacttcca caggataatc gctgttacct
    16381 gcttttaggt aagccaagta gttctctagt gcctctggtt cctggtttag tattttcttc
    16441 gccagtgctg aagcagcaga aaagccagtc gagtattgga aaacatagta attataataa
    16501 aaatgcggta tccgtgacca ttcgaattta atttctgggt cttcttcgac tgctggacca
    16561 taatattttg cattcaattt accataacta tcacttaggt attcactagt caatggcaca
    16621 cctttttcat cttccgtatg catgaaatgc tcgaattcag cgaattgtgt ctggcggaaa
    16681 actgttcctt taaacccatc caagtaatgg ttgagtacat aagcccgtac acgagggtct
    16741 ttttctgttt ccaataaata ctccgttagg atattttcat tcgttgtcga agcaatttct
    16801 gctaaaaaga tggagtagtc gccgtacaca taaggctggt tcgaacgagt gaaataacta
    16861 tgaacactat gtcccatttc gtggacaagc gtaaatagct gatccagcgt atcatgccaa
    16921 ttcaataaaa tatatggatt tgtgtcatag cttcccgaag aataagcacc gcttcgtttc
    16981 cctttatttt cgacaacatc gatccaacgt tcagagaatg ctttttctac gatggccatg
    17041 tattcttcac ccattggttt cagtgcttct aaagcttttt cttttgcttc ttcgtacgta
    17101 aaggtaattg gagcttcccc taagaccggt gtataaagat catacatgtg cagtttttcc
    17161 acttctaata aacgtttccg taattccatg tatcgatgta acaaaggcaa atgcttgttt
    17221 accacgtcta ccaaagtatc gtatacactt tcaggaatat gattattgct caaagacgct
    17281 tctctggcag agctgtaatt acggactttc gctttaaaat tatgtccttt tatatgtgtg
    17341 cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa cactgtacaa tcccttaaat
    17401 gctgcttcgc gcacccttcg atccgtgctt tctagcaact gaccatacac gccatgagat
    17461 aattggacta tttcaccatt ttctccttca atcgttggaa aaactagatc tgcattattc
    17521 aaaacagcaa atgtatcact tgaagcatca aagatttcac ctgctccagc aagaagagat
    17581 tcctgctcag cagataagac atgggctcga ttatctacta tttgctggat ataatggcga
    17641 tagacttcca attttggttc ttctttgaaa tattgccaaa tctggtcatc tgacaattgc
    17701 aatatttctg gttcaaacca ggaaacggct tcactaactt tcgaaaacag actgcttgct
    17761 cttgcataaa gcgcttggta atctgtattt ccagtgtctt gatcgttttt aaggtgcgca
    17821 tatacataaa tgacttcagt ttggcgataa accctcaata cgaattcaat cgcatttaaa
    17881 aattgagaag cgccttgatc aagtgttcct ttgtgttttt cagattgttt taactcttct
    17941 gataattcca aatatttctc atcaaactct tggtcgcttg aaaagatctt ggtcaagtcc
    18001 caagttaaat tttcaggcaa ttcttctcgt tttggcaact gctttacttc catgaaatcc
    18061 actccttttc ttcccttagc tctatctatc aaaatagatc atagtctaac atccacatgt
    18121 cacagcatct aacttttcta ctcattcatg tgtctgcttc catcaatttt agcataacta
    18181 aatcttgttt tacatagaaa accgattcct agcctatttt tatacttact attttttcaa
    18241 agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag accatctctt catcaatcat
    18301 aggaaaaagc cattcccttt tatgttcttt tatttggcaa agccatgagt tgaaatcagg
    18361 aactttctgg ttttccaagg cttcttcgta caacattcga taaagaaaaa cctgttcttg
    18421 taaataaaag aaaaaccgac ttttttgata gatccacgat gatagatgca aaagatgttt
    18481 atgacgcaag tagcattgtt cttgtatgga tactgtattc ttttgcttgt gaataagttg
    18541 gcgtgtcagc catttcttcc catcttcact cactttttct atttttttca tttggttcat
    18601 tttaatttcg ttactagtga atatttcttt aaggtcccta ctagaaaaag tccagcagct
    18661 actgcaaaag gaaatatgcc ctgatacagt ctctgtaata tgatgataca aataaagctt
    18721 ttgtttcttg agatcagctt tccagcaatg cactcctctt tttctattat agtagcagca
    18781 acttttttct atcaatgaga attgcgattg tccaagaaaa gaattaccaa gtatccacca
    18841 tacttggtaa ctgttattca aataattttg ggttctttca ataaatctct ggtgagacag
    18901 tctagaacat tggatttcta ttgctagatt cgccaacaaa atatccggcc tttgccgcaa
    18961 cctcggcaaa taggcttcca aaaatactgg ttctactgac tgtcccagcc aatccatgaa
    19021 gcattccttt aactgtaagt gctcttttga ttctctttca ctaaagaccg aacagttatc
    19081 tgatttttga tgggcaaaat gagcacattt tttctttccc tttttcaaaa tcactctttt
    19141 tcgacagcca cgacaataat atttctttcc tttttctgca tgtttagcaa aacagcactt
    19201 attatattca ttcattgcaa gcagcatttt tctccctctg aacttattta cgtctaagaa
    19261 tttatttaca cgaaaaaaga gggcgtgaaa aagctattct gcatcaagta ataagaacag
    19321 ctaaacaaaa atagttccca atatttttcg tttggttggg cttgttaccg cagatgcaag
    19381 cttttgaaca catttatacg aaaaaaagac tgcgacacaa tttttgtcac agtctcttac
    19441 tataatgctg atttatacaa aataaaattt cgttcgctcg atagcattac gcgccatcag
    19501 acattgtcca tattcattca atgtttctgg tgtacgatcg ctcttttcag caaactccaa
    19561 aattcgagca atctgattct ctacatccgc ttggttgaga ttttccatcc agaagtgaac
    19621 gactaggtaa tagcgctctt cgaataaata caaatcagtc caagcagact ctagttcgac
    19681 atctgttgca agttggatca cttgttcaaa atcttcaaaa gcaaaagttg cttgtctttc
    19741 ctgttcttct agggcgcttc cagataattc ttcctccgga tcgctgccaa taatctgctg
    19801 acgaagaaag tcactcacat cttctgaatc aacagtagaa tcttctgatt gagaaatttc
    19861 ttcatccgga agatttttac tgataaacag ttctaatccg tctccttttg gcaaaacttg
    19921 gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata tcaacttctt ccaaaatact
    19981 ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa tccaaaaatg aaacgccccg
    20041 ttcctctaaa tcttcatgac caattagtac acggatcgta ttttcattaa tatgttccat
    20101 ttccattttg ctacacctca ctttgccaca atgtttaata tagattcatt gtagcgaaat
    20161 cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga atttaataaa accagcggat
    20221 ttttcaacct ttcaagctat cttatttaac tataacattt ttggcagacg aatactatat
    20281 agccgacttt tcagctaatt ttccctctct atttttcgtg caatcatcat cttctactac
    20341 ctttttatac atctaatcaa gaatactttt tgtaaaaaca aaaaaacttg gaaaaactat
    20401 ctttgtaata gt
//
LOCUS       DACDKJ010000052        20183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDKJ010000052 DACDKJ010000000
VERSION     DACDKJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.052"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(153..650)
                     /locus_tag="IV328_002029"
     CDS             complement(153..650)
                     /locus_tag="IV328_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8938146.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(780..1490)
                     /gene="budA"
                     /locus_tag="IV328_002030"
     CDS             complement(780..1490)
                     /gene="budA"
                     /locus_tag="IV328_002030"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8938147.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1503..3167)
                     /gene="alsS"
                     /locus_tag="IV328_002031"
     CDS             complement(1503..3167)
                     /gene="alsS"
                     /locus_tag="IV328_002031"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8938148.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3375..4037)
                     /locus_tag="IV328_002032"
     CDS             complement(3375..4037)
                     /locus_tag="IV328_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8938149.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4047..4862)
                     /locus_tag="IV328_002033"
     CDS             complement(4047..4862)
                     /locus_tag="IV328_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8938150.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5123..5566)
                     /locus_tag="IV328_002034"
     CDS             complement(5123..5566)
                     /locus_tag="IV328_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8938151.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5700..6038)
                     /locus_tag="IV328_002035"
                     /pseudo
     CDS             complement(5700..6038)
                     /locus_tag="IV328_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6026..6403)
                     /locus_tag="IV328_002036"
     CDS             complement(6026..6403)
                     /locus_tag="IV328_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8938152.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6627..7820
                     /locus_tag="IV328_002037"
     CDS             6627..7820
                     /locus_tag="IV328_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8938153.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7986..8408)
                     /locus_tag="IV328_002038"
     CDS             complement(7986..8408)
                     /locus_tag="IV328_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8938154.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8991..9485
                     /locus_tag="IV328_002039"
     CDS             8991..9485
                     /locus_tag="IV328_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938155.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9647..10819)
                     /locus_tag="IV328_002040"
     CDS             complement(9647..10819)
                     /locus_tag="IV328_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8938156.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(11025..11840)
                     /locus_tag="IV328_002041"
     CDS             complement(11025..11840)
                     /locus_tag="IV328_002041"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8938157.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11990..12436)
                     /locus_tag="IV328_002042"
     CDS             complement(11990..12436)
                     /locus_tag="IV328_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8938158.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12433..13437)
                     /locus_tag="IV328_002043"
     CDS             complement(12433..13437)
                     /locus_tag="IV328_002043"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8938159.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13477..13806)
                     /locus_tag="IV328_002044"
     CDS             complement(13477..13806)
                     /locus_tag="IV328_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8938160.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13932..16259)
                     /locus_tag="IV328_002045"
     CDS             complement(13932..16259)
                     /locus_tag="IV328_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8938161.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(17102..17395)
                     /locus_tag="IV328_002046"
     CDS             complement(17102..17395)
                     /locus_tag="IV328_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8938162.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(17798..18976)
                     /locus_tag="IV328_002047"
     CDS             complement(17798..18976)
                     /locus_tag="IV328_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8938163.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(19080..19394)
                     /locus_tag="IV328_002048"
     CDS             complement(19080..19394)
                     /locus_tag="IV328_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8938164.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            complement(19714..20109)
                     /locus_tag="IV328_002049"
                     /pseudo
     CDS             complement(19714..20109)
                     /locus_tag="IV328_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         5908 a         3982 c         3455 g         6838 t
ORIGIN      
        1 caaaatgata ataccattaa aatatgattg tgaaaatcaa taacacttgt gattttataa
       61 aaaaataaaa aaaccgaaaa acagaaatga ccactgtctt tccggctctt tttattcttg
      121 atttctcttc ttttttgttt caatagacga ttttagttat taataaatga aaaagcattt
      181 atattaaaat ttgctaataa aagatctaca tatttttcta gaaatgcttg atcgatttca
      241 tcataccctt tcgttactcc gcaatcgata tctaaaacac cgattaaatt accttctttt
      301 accattggca ctacgatttc tgattgtgcg gctgaatcgc aggaaatgta atttgcatgg
      361 gttttgacat catcaacgat caatgtttct tgtttttcag ctgattctcc acaaactcct
      421 tttcccatct tgatacgtgt gcaagaaact cgcccttgga acggtcctaa aatcaattca
      481 ttaccatcga ataaataata tcctgcaaag acagtctcag gcaaagtctc attcaacaat
      541 gcagatgaat ttgctaaatt tgctaaccag ttatcttctg cttctaataa tccttgttgc
      601 tgtaataata aaatttcgta tgcatctttt ttctgttttt ctgtccacat gataaaaacc
      661 gctccttttt gatctatgga gaagattata gctcagtttt tttgtaaacg ctaggtttcc
      721 tcctcaaaaa aacgagatac gtcaggttaa tcaccttgaa cgcatctcgc tgtctttttc
      781 tattctgctt gttggatttc tgctgctaaa tcagcataat caatcgtttc gtgaacaaaa
      841 gtcggatcat caactggaaa atgctgtaca agggacgaga tgcggctaat ctcaactttt
      901 ccttcagcca cttcaaaatc cattacatgt ccgccaaatg tatgtgtatc atcgataaaa
      961 tgcaggtgaa atcccgctgc agcaactcct tggaataatt caggcgtaaa gaacccgact
     1021 aatgttcctt ggatattgct ttgttggaat tctggttgag actctgaaat ttttgctagt
     1081 cttctgtaag gtttttcttg cttcggcatg atacggatgt gcatattttt gaataatcca
     1141 gttatcttca ctgccaaaaa taggttttcc ccggcttctc ttactaatat gtcttttttc
     1201 aattgttctc catctgcagt ttttgagaca gaaaaactag tatctggcgt aaaatctgta
     1261 attgctgcat agggggtaag ttcacttcca tctaattggg caactgagcc atctgatctt
     1321 ccttgataag ctacgccatc taaaaagatg acttcaccat caagaccatg caatgttccc
     1381 aatcccagag ttcctttttc taatatatga gcaatcgttt ctgttccatc catcaagccg
     1441 gccatcaaag ctcctaatgt cccgtgctgg tataagattt tttcagtcat gtcgacactt
     1501 ccttaataga attgatctgg taagattgtt tctcccaatt tctcattatc acggtaatca
     1561 atcggaatat caataattac gggtccttct gtcgcaaatc cttcttctaa agctttttct
     1621 aattcctcta cacttgtagc tcggattcct ttagcgccaa aagcttctgc atatttcaca
     1681 aaatcaactg gaccaaagtc tacacctgac gcccgctgat atttcatttt ttcttggaac
     1741 tctaccatat tgtagtgtcc atcattccaa atcaaatgaa cgatattcaa ttttttacgt
     1801 accgccgttt ctaaatcctg ggcagagaat aaaaatcccc catcgccgga aactgatacg
     1861 atttgtgtat ttggacgcac caatgcagca gaaatcgccc atggcaatgc aacaccaagc
     1921 gtttgcatac cattactgaa taacaaatga cgaggttcat aacttctaaa gtggcgtgcc
     1981 atccaaatat aatgactacc tacatctacg gtaacagtca tatcatctgt tacttttgac
     2041 tgaagcgtgt taataacttc taaaggatgc aagatacctg cttctccttt tgattgaaca
     2101 atgtcccgct ccgttaattt tgcttgaaga gatgctaaat attcttttgc atcttctgac
     2161 acttgttgag gctctaatga acctgtcaat aaatcaagag tggctgagat atcaccgatc
     2221 aattctcttt caggctgcat aaaaggatcg atttcagctg gcgcttcatc gatcacaatg
     2281 atccgcgcat ctttttccgc attccagttt cttgcttcat attcgattgg atcatagcca
     2341 attgcaataa ctagatcact tcgtttcaaa agcatatctc ctggttggtt tctaaataaa
     2401 cccacacgac caaagaaatg cgcttctaat tcacgtgaga tcactccagc tgcttggaat
     2461 gtctctacaa caggcaatgc tgtcttttca actaattgcc tgatggctaa cgtttctttt
     2521 tcacttgatc ctcgcatacc tactaatagt accggtagtt ttgcttctct aatgcgttca
     2581 gctaggtaac ggatatcatc tgcagaagca gaacctaatt taggatcgct caatggcttg
     2641 atgacattac cttggacagg tgcatccact acatcttgcg ggatactgat aaagctcgcg
     2701 cctttttttg aactcttagc catacgataa gcattggcaa tcacttctga aattgtttct
     2761 ggatcttgga tttctgcact gtattttgtg attggctgga acagtgctgc attatccatg
     2821 ctttgatggg tcaacttcaa tagatcgctt cgttttactt gtccggcaat tgctaaaact
     2881 gggtcacctt ctgctgtcgc tgttaccaaa cctgtagcta agttactagc accaggacca
     2941 cttgttgcga taactactcc tggctctcct gtaatacgtc cgattgcttg tgccataaat
     3001 gcagcatttt gttcgtgacg agtcacgatt aattcaggac cttgatcctc taattcatta
     3061 aatacaccat cgatttttgc acctggtata ccgaatacat aatcaacatg atgattaatc
     3121 aaactttcta ctacagattt cgaaccttgt ttcatttttt cagccattga acatcgtcct
     3181 ttttatgctt tatttttaat tattagttag aaataatcac aaataagtga tacagtttct
     3241 aaacctgttt catacaaaag tatagcacgt tttatttcat ttgtttagcc atatgtttca
     3301 aaaaatgaaa aagaatgaca gtttttacat aaaaaaaact gtcattctta attgaaagtt
     3361 tcaatttata aagtctacag atccatagtt ctcatcaatt tacgattgtt gaagtaaatc
     3421 aaaccaaata cgatagaagg acctagtaga atacaccatt ggggctttac gaaaaagaag
     3481 agaaacaaac ttgtcaatag taaaaaatct agtaaactca cataccattt tttcaacgta
     3541 tagtagaacg ctatccgtag aacttgattt ggtttcgacg ctggatttct cacctgaaaa
     3601 taccagcagt tggtgctaaa ggaaactgat aaacaagcaa gtaaaacaaa caacggaatc
     3661 aaccattttc cataagaaaa acgtataaaa aagaaacaat caaaaatcat tatcacacta
     3721 actatccaag caaatagcca ataaaaaaat ccctttagcc aaaattggcg aaacatcttt
     3781 atgaacaaac gtactggtga aaaactattt tcttttgcta attcttctgt catacctatc
     3841 atcacgatgc aacctggacc aataaaaagt aaagaaaaaa taaaggaaaa gacattcctt
     3901 ccatctatcg acaaaaggaa cgcagctgct atatacaaga tattgacaca ccaaaaacaa
     3961 aggctacttg ccaatgattg atacaaccaa taaaagaatt ccatataaac atttttgtca
     4021 aaacgctgct ctttcataat cacctcctat tccttcattc ctgatgaagc gatcccttct
     4081 acaaaatatt tttgcatcgc taaaaatata aaagcaacag gaagaacaga aacaactgag
     4141 gcagccatga ttaatgcgta ctctgcatca aatagcccaa taaacatttt caaccctaac
     4201 tggattgttt tattttccgt agaagacaaa taaatgaatg gccccatata atcgttccat
     4261 gtattaacga atgtgatgat cgtcaaactg gcaatcgccg gttttgtaag aggtaaaatg
     4321 atcttgcgat agattcccca ctctgataag ccatcgatcc gcgcactctc acataaagaa
     4381 tctgggatac tactgtaata ttgcttcaac aagaacacac caaaagcatt gaacgcttgt
     4441 aatagcacca acgaccacaa cgtatctgtt aaacccactt ttctcatcat aatgaactgc
     4501 gggatcatat aagactgcca agggacagca atcgtgccga tatatgctaa aaataaaata
     4561 tcgcgccctt taaaatttag tttagaaaac ccgtaagcag caaaactaga agtaaacaac
     4621 tgaatcaacg tgatcacaac gcttaaaaaa gctgtattct taaaaaatgt cactaaagga
     4681 atacgagacc aaataaccag ataattctct aaatgccaag ttttagggat ccattgaatc
     4741 ggaatcgtga acacttcatt atttgctttg aatgaagcag aaaccatcca taaaaaagga
     4801 atgatcgtca ataaagctaa tacaatcagc agaatcatac ccaaccattg aataaagggc
     4861 atgttttttg aacgaatttt tgcttttttt ctttcatttc gaacacctcc aaagttataa
     4921 tcttcatttc tttgtaatga ttacaatcca gaacaacggt tattatagct gattcatttc
     4981 cgttcttctt atttttgtaa gcgcttattt aacaagttat gtaaatctct ttattttttt
     5041 gatttaaaaa tgaaaaaata caaaagcaag actgtcatac gtctcgcttt tgtattgaaa
     5101 tttcttcgat tagagatact tattactgtt caaaagcttc tttgaaacgg tgaatcaatt
     5161 cattgactac aggtgataaa atccgattct ttttccagac tagcatgcag ttcgtttcaa
     5221 tcattgggtc aagcggaacg aatgtcactc gctttgaatg atgacttaca gcaccttcta
     5281 tgactaaagc agcacctacc tgattctcaa ttagcgcaaa tacattgaaa atcaaattgt
     5341 acgttcctgt gatcagcaac tgctcctttt ttatgcctaa ccagtcagca aacattcgct
     5401 gtatttctgg acgctttgag atcaaaagag gtacattcgc aaagtcttct tttgttatca
     5461 cgtctttttt tgctaaaaca gaattttcta aaatgagcaa tccccatttt tcctttcttg
     5521 gtaacaaaat cgtttcgtat ttctctgtat ctactggatc tagcaaaatc gctaaatcta
     5581 acaggctttt ttctagtcac tccgtgatat catcacttgt tccgctgtgg atatgaaatt
     5641 gaacgctggg ataatcacgg atcaactttt ctagtaatag tgccaatgta ttcgaactat
     5701 cagcttccac acagccgatc gtaaatcggc cgctgaataa ttgttttttt ctgttctcga
     5761 attcttgagt ggtttgattc gttaaatcta aaatttcttc tgctcggctt ttttagaaaa
     5821 taacctgctt ctgtcaagat cattttgcgg ttttcgcgta caaacagttc tgttcctagt
     5881 tactcttcta actccttcag ttgtcggctt aaggttggtt gtgtaagatg tagaacttct
     5941 gcggcttgag agattgttcc ttcttctgca attgtccaaa aatattttag taagcgtaac
     6001 tccatagcga tgctccttga tttttttact tgatacattc cattttattt ctagataaca
     6061 cacgatcata tttcttgatt tttccttgta aacgttccaa acattcttgc atttcagcaa
     6121 tttttttcgt caaaagttca ctttcttcaa tcaaaagctt acgccgctca aatacagttg
     6181 cttctccttc gctagatagg cgaatatatt caatcaaaga ctcgatcgat aatccggcat
     6241 ttcgcataca gatcgtaaag tcgacccatt ttaggtcttc ttctgtataa tcacggattc
     6301 cgccttttgt ccgattaact ggtggaatca acccgattcg ttcataatag cgcaatgtat
     6361 cggccgaaat gcccttttct tcactgactt gtttgatatt catctgatca ctctttctct
     6421 taatttcttg ccactttact tatgtatttt atttattcta caactttaag tgtactccaa
     6481 gtcaatactc atttctatta aaactaagct ggattatcta tatcggttgt ttgtgtatta
     6541 tttcaaataa aaaagtcttg tttttttatt cttttttgga tatactttta acaatttagg
     6601 gaaattttag gaaaaggggt ataaaaatga caacgtatca gcgaaaggta ttagtatcga
     6661 catcggcggg aattgcttta gagaatatgg atattatgtt tttagctttt tcattatctt
     6721 caatgattac ttcttttcat attagtggta cgcaagcggg tttcatttca acgatcacta
     6781 atttggggat gctcattgga ggtatttttt ttggcatcat agctgataaa tatggacgtg
     6841 tgaaagtatt ttctcaaacg gtccttctgt tttcgattgc ttctttattg atgtattttg
     6901 cttcaaacat ctacttggtt tatctctttc gctttatcgc aggtgtagga gcgggaggag
     6961 aatatggggc gtgtatgtcc ttgatttccg aaagtttttc aaaaaaacaa attggacgag
     7021 cttcttcggt tgctggtatt ggtgcgcagg tcggtgcggc tttggctgcg attttagctg
     7081 ctgtgatcat tccaacgttt ggttggaaga tgttgtatgt cgtaggtgta ctgccggtgt
     7141 tgatggtctt gttcattcgt cgcggcttga ctgaaccaaa ggaattcaaa tctgtacaat
     7201 ctgccgaaaa gaaaaccaaa cttagcagac tgtttgaatc aaaacatctt gcttggcaaa
     7261 catgtggcct tagtatcatg gtaactgtcc aaattgccgg ctattttggg ttaatgaatt
     7321 ggctaccttc aattatgcaa aatcgattag ggctttcggt ttctgggtct tcattgtgga
     7381 tggtcagtac tattttaggc atgtctttgg ggatgatgac ttttggcata atcatggata
     7441 aattggggcc taaactagcc ttttctttgt ttttaatttg ttcatcctta tctgtctatc
     7501 tacttgtgtt agcccatagc cagtggagtt tggtagcagc cgctgtgata gtcggctact
     7561 ttatcaatgg catgtatggt ggatatggcg cagtgattag tagtttgtat ccaacagaaa
     7621 tccgcgcgac ggcgaataat ttcgttatga accttggacg agctgttggt ggtttttctt
     7681 ctatcgtcat cggtttcttg atggatcatt atagtttaac tgccgtaatc ttgtttttga
     7741 gcggtattta cctagtcagt ctgctggtct tgacaactct tactggtgtt acagcattaa
     7801 gggaaagctt gcagcattga tagttgagtc tgtaaatgac aagaaagagg tgtggaaaaa
     7861 gcttgcatct gcggtaataa gaccagccaa atcaaaaaca tgagacactg tttccgtttg
     7921 gctggtcgta ttacttgatg cagggcagct ttttcaactg ctaatttcct tctatctggc
     7981 ggcgactatt ctttgacgtc cgtcatttca aagtacttta ctaataactt agcataacga
     8041 aaaactgtgg atacactcgt atattgagcg tctgcataat cttccaaatt tttctcagga
     8101 tgttgataca tgaattcaat caaatgataa ttatatgatt ttctgtagaa atgaagataa
     8161 ataatgtaga ttgggaagtt ctcctgcttt tccatagaaa agcgccgatt attctcattc
     8221 ttcaaaatga ccttatcagg atattgttgg aacaattctt ctaaatctgt tagataattc
     8281 aagatagact tacttgatac cttcaattcg ttttgtagtt cttctattga acaccaacgt
     8341 tctgtttcaa gcagtaaatg aatcatttta attttctttt gagaaacttt tgttaaaaaa
     8401 gtctgcatat tttctcctcc tgtatctact ttactatact acatacaaga aaaaaacgga
     8461 tacaaatatc atattgtcac accgtcaata gtgcataaac ctgttattat ttctcttttt
     8521 ttcctaagta ttctattgat atgaatcatt taatcgttta ttatataaat gtcaataatc
     8581 taataataaa ataacgtaca tattttatta ttgcatactt aataacagaa ctatagcaaa
     8641 aaatcccgtg tatcaacacg ggattagatt aagtttcctc aatcactatt cataacacaa
     8701 ttattataac aagtaagtaa acaattgaaa aggacattta ttgaaaacgg ttgtttttta
     8761 cttaaggagg aaatttagtg aaaaaattag ttgcgttgtt tttggtcgca ggaagtttgt
     8821 tgagtgggtg tggtgctact acgtctacta ctcaaagcac aagttcagct caggcttcca
     8881 gcagcagtac cacgatctcc gaaagtacgg cagattcatc gaaagaacaa attgaaaaga
     8941 aaaaagaaga agagaaacaa aaagcggaaa aagctgcact agaaaaaaaa gtgacagaag
     9001 cggaatctgc tttgtcacaa gctgaagcaa atcctacaaa agaaaactat gcaactgctt
     9061 cagcagctat ccaagcgcta cctgaaaaca aacaagagtt gacaagccgt cttgctgcaa
     9121 ttgattccac aatcaaagca aaagaagcag aagaagaacg gaaaaagcaa gaagaaattg
     9181 cacgacaaca agcagaacaa gcgcaacaag aagcaatcgc acagcaacaa gcagaacagg
     9241 cgaggcaaga agcaatcgca cagcaacaag cagaacaggc gcgacagcaa gcagcagctg
     9301 aacaggcttc tcagcaggca caagccgcag ctcctgagca aaataatgaa caaactgtct
     9361 atgtcacacc gaccggttca aaataccata cacataaatg tggaaatggt acgtataccc
     9421 ctgccacttt aagcgaagct caagctcgtg gtttaacccc ttgctccaaa tgttatggaa
     9481 actaattcaa aagttatact ataataataa aattcttctg aaacataaaa aaatagaagc
     9541 tggcgttcca gatagtaaac caaaaatagc agtttacacg tccatttcgt cagcttctct
     9601 cttttttcat ttagtctgtt ccctctgctt ttttggacat cttcattcat tctttctcaa
     9661 tcaaatagta cacctctttt tcttttcgca gtttatatct tccttttttc aatttcattg
     9721 aaaactgttt cgaatgttta tgttttacat ggattttaag caggagctcc tcatgttgca
     9781 agcgatagtt cctgcctttt aaatcagaaa atctcactag tccgtattca tcagaagctt
     9841 tcacaaatgg aactttttct ctagtaatat agtgtttgcc gttgagcgtt tttacagaaa
     9901 agaccattcc ttcgattggt cgtccgtttg tgttctttac ataaaaagat aaaggatagt
     9961 ttcgcttagc gatggccaaa gaatctaaat aagcgatgag tagccaaact aagccgattg
    10021 atccaagtag tataccgata aaccaaataa aaaatttcca ttttttccat tgatcaattc
    10081 cttggatcgc tttttttgcc tcttttgctt gataaggaat acgatgcccc gtcacaagca
    10141 gtcgatgtgt attgaccata taaggagtgc aggtaagcaa agtcacgaga tctctgcctt
    10201 tttggatacc caaagaatct acttcgtcag gtaagaccac ttggattttt tctacttgat
    10261 acgccaatgt ctttccattg atctggatga aaaactggtc tccttttttc aacttaggca
    10321 aatctgtaaa taattttgct tctgggagtc cacgatgccc agaaagaacg gcatgggtgt
    10381 ttttcccgcc agtcggataa gaacttcctt ccaataatga cgttcctttt tgcaaaaata
    10441 tttccgttgt ctgatcgaat ataggtaagc tgacattgat ttttgggatt tcgatgactc
    10501 caagcatatg ttccataaaa aaggcattac taggtaaatc ttttaatgct tttgcatcaa
    10561 ctgcttggtt gaaactgcct atccctggtg atacgttttg ctctgccaac tgttggtttt
    10621 tctttgtcat tttttcctgc tgttttttta tttcctctga attcttattc gatgcttgct
    10681 tttgataatg gatgatcatt tgctgggcaa gaaaatcatt cagtgaatct tctacaaaag
    10741 gataagaaaa gatcccgatc ccaacgacta aaatgattgc catgaagatg tccagtattc
    10801 ttattctgct tttattcatc atgtccacct attttctaat atgtatatga agaggttgtg
    10861 aaatcagcgc cttgacctga gtattagcga agcaaacggc aaaatagttc ctcctatttt
    10921 gccgtttgtt gagcgaatac caagagaccg accacttgtc acaacctttc tccccatttg
    10981 aatcactatc gtttatttat cgtatgattt ttgtttccaa tagtttaagc ttctgtttct
    11041 tgatggcgtt ttttgaaata gatcactgct cctccaacta aagcagtacc taccaagatg
    11101 aatacgatga tccccatacc accagttgaa ggcaagattc ctttaggttg gttatcgatc
    11161 gttgtttcat cagaagttgt tggatcatca tctactgtaa attcagtgta cgcagcttca
    11221 ttcttaggta atgcgtagcc ttctggtgct tttgtttctt gaaggcagta tgccccttgt
    11281 ttcagccctt cgattgaaaa tttcccttct gaatcagaaa tcaatttcac ttcttcccag
    11341 tcagaacctg tgacttcatc tgaccaagca tatgggtatt ctcctgaggc aaggtttgct
    11401 atttttttca ttaatttaac agttggatct tttgctctta gaatagaaaa ttctgcatcg
    11461 gctaattttt cttgcgtgtt cacatcttgt ttgatgaagt ggaaatcacc gtagttttgg
    11521 acattttcta cagtaatcgt gttatccgta gttgtatttt cccgattgac tacaccgttt
    11581 ggattcccaa gattggcttc atctgcgcca tggatgatac cggttgtttg ttgcaatttt
    11641 ccgtttttta ctataaaagt agtttctttt tgtggattgg ttaattgata accatctggt
    11701 gcttttgttt caactaaagt ataattgccg tctattaatc cattgacact gatggatcct
    11761 tcagtatcag ttgtgaatgt caacgctgac tcagcaggat catagagagc gacaccagtg
    11821 ttttcatcaa aaccggtcac gaattgattc tctgaatttt tgatctggaa ttgcgcccct
    11881 gctaacttat ctacccctga aaagttccca gctttctgga ttttgatatt gctttctttg
    11941 atgacatttt tagcatatag gtgaatcgtg tctaaaggtt tgtcagggtc tagggtaagc
    12001 ttaacacgat atttggcgac gtttcgacac cgggctttgg tgtttctaca aagacgtata
    12061 cagcatcttt cccatcagat ttacctggaa gagtcaggtt agctaatcct tcaccagaag
    12121 tcgttacact tgcgaagggt gtagcgtctg ctggaactgc tgtgtcttta cttggatagt
    12181 acgtagcagt gatgtcatat acgttgaatt caacgccgtt caacggttcg ctattcggaa
    12241 atgtcaattc cgttccgttg tttatttgat tttctggtgc tgaagtgaat ttcaatttat
    12301 gcaaattaac cgtaacattt tctggtgtag gtgatacggc tgcagaaacc tctgcagttc
    12361 ctaggccgag aacgaaagga agcagcatga tagcagttcc taaaaacgtc catatttttg
    12421 ttttgatgtt tttcatttta tttccctcct attgtttcct ttttgttgtt ttttataaaa
    12481 ccaagctgtc cctgttgaaa ggactagcaa taaccctaaa attgagaaac ctccaagcat
    12541 ctcttcgctt gtttttggaa agcttcctcc atttttttca tttgatggac catttgtctg
    12601 catctgaact ggcgtttttc cattattcgt cggatttgct tttggttcgt tgatgatttc
    12661 caatggtata ggatgaagcg gatcataact gcctttttca acttcaaaga gaatggttga
    12721 ttgactcaat atatatcctt taggcggttt aatctccact agttcgtaac ttccaaaaga
    12781 caatccatgg acagaaaatg ttccatcttt actggaagta aaaatcgttg ctcttgaatc
    12841 atctttggct acccaatgat ttatcccgtt ttcttgcgct aagtattggt catttttttt
    12901 gactagaaat tgagcatctg ctagtttttg tttcgtttgc cgatccactt tgacaaattg
    12961 tttgcctcct gtttggatgg caagatgtgt ttctactgtt tgattgtcag tcgtcaaacg
    13021 tacatcgtta tcaaatgtcg ttttttcact gttttctttc aatgtcattt ggtaacggat
    13081 cgttaatttt tttccagcaa acgaactaag atcttttcca gaatctgata agacaaaacc
    13141 atgttttatt ttttgtgtgt gaaatgtatg aatcttgtct ccaccaatca aaaaatcgat
    13201 cgtttctggt tctagccata actgaggatc agctgtatct ttgatcgtat aggttcctat
    13261 ctcgttgatg ttctcaggaa ttgttacggt gatgagataa gggactttat ccccatctgc
    13321 aaaatctgtc tgcttattct ctactgtttt tgttaatgtg agtgcagcag gtattttttg
    13381 ttcgctcttt ggatacaagt ggatggtcgt caatttatga ttagatgaat ctgtcacagg
    13441 aagcacaacg acaaatggtg ctgatttttc ttttacttaa tctgaagtct ttatttccgt
    13501 aaatcggtaa acagcgtcac gaccttgctc atctttgtcc gatgcactaa aagaagcaat
    13561 gccctcttcc tcgtctactg tttttgtcac attttctgcc agtatttttt cactatcact
    13621 tttttgtgct agttttcttt gtgcgtcttc cacactactt ccttctgacc gcagcttgta
    13681 aaattcatcc gagacatcat atatagcgaa agtaacgccg ttcaaccctt ttactccttg
    13741 aaagttatcc gggatattta ttccgtcgtt ttgactgata cttggcatct tatcgctttc
    13801 gaacattcga ttatgcaaag taaatgtaat aggtgattct ctagctgaca cagtcggaga
    13861 acctataaaa aagaccagta ggataaaaaa gagaacagca aatatatcca cacgtacatt
    13921 ttttgttttt ttcatcgctg ttcacctgct tttttcttac gatggataaa ataaaagaag
    13981 ccagaaaaaa taaagatcag tcctaaaaag acaaagggca gtagacctat cccacctgtc
    14041 gaaggaagca agagagtcgg attgatcgtc aacttagcgg tatctgttgc gataatcgta
    14101 taattattgt ttacacttcc ttcgcctaaa ctaatcgtta ctatttgtga tccaatatct
    14161 gtccggctgt taactgttag ggtataatcc aatgattcgc ctaagacaac accggtaacg
    14221 tggagatttc caaccgaaat cgagtgactt tgggcatcat aattaaaggc agcgtcatcg
    14281 acagtaatca ctgcgttagc tttatagatg gtataagttc ctgatgtgtt ggctatatca
    14341 gtaaatgaca acttgggatt tgctgctttg actttgtcta ttccgctatt cgttagttta
    14401 attgtatagc tgccagcatt aaccatattt tttgctgatt gtgcatctgc tgtgtaatcc
    14461 atactgctaa gtgccacttg tactggagta agcgaactag tgacaccctc tgctagcttg
    14521 acatggattg acgggacacc tgcttttcca tcatatgtct tactgccact gagctctacc
    14581 gcaccgctga tttgcttaat cgtatagaca tcagatgtct gcacgatagt aaataaatag
    14641 tttgttctgg cagctagttt agctagtccg ctttttgtca attgccaaga cgtataggtt
    14701 ccaatttcag taggcgcagt tacttcaact cccttttcat tcatcgcttt gaaatcagtt
    14761 ggattccatg tgatttgatc aatactgaga tcattgttta aaagggatgt tgattctgtt
    14821 ttatcattaa aggcatattt aacgatcggt acaacagaaa gaccatcata gtacttactg
    14881 ccatttgcat tgattgatac tgttgcattt gtcttataat ataggatgac ttcagaaatc
    14941 tgttttccat ttgcgtcctt tacgattaca tttccatttg tatctaaact agctaaaaca
    15001 ctcattgctg attggttctt tgtttctatc gctgaaacag ctgtaaaggt atatccgttg
    15061 atagtaggcg tattagtcgt caggtcttgt gcagcacttc cgtaagtaag attcattatt
    15121 ttagaaggag caatcatctg tccaaaatag tcttgatact taatagtgat tggtgtttgt
    15181 ttgcctgtat aggtcaccgt atcgacgtta ctttctaggg ctgtgaaaga accgtttgtt
    15241 tgtgcaaaag aatagcccgt tactgctgtt ggagtgataa ccactttgtc acccacatgg
    15301 attccagata atgtagtccc gtacgttccg atttttgctg ctccaccatt gactgtataa
    15361 gaaaaagttc ctgtccgatt cgcattgacc tgaaccgttg ctgcttgata ggttggaaaa
    15421 tttgaaacat ttcccttacc atcaacgatc tgcccatttt tgatcgagta ccccgtgaat
    15481 ggaatatcat ttgctaactg tataccgatt gaaaaagtac tcatacctcc tccggcagga
    15541 agccctcccc atgttgaggg tgtaaaagaa taacttttat ccgcattggg tgtaactgaa
    15601 tcgatcgtat ttccagcggt agccaccccc atatagatac tttttgggaa tgtgatagta
    15661 gtaccattta ctgtggagcc aataaacaga ttatggatat ctggacggtt atcaataaaa
    15721 gcgattgctt tggttacgcc ttgtaacgca gttttatccg cactcatcga tccgttaacc
    15781 gcaacaatga ctccatcttt ggcatatatc actgtatatc catctggtat ggtatgtccg
    15841 ttgaaattcg cagtggccgc aacagtacta aatgtctggc ttctacctgt ttctgaataa
    15901 gctttctccc ctgctgcttt tgctgctgtg atttcttttt ctgtcggatc aatcagttga
    15961 gtcgaggatg tggttgaggt tgtctcgttg ttgctagact gattggtgct ttctatggac
    16021 tgtatttcag aatagggtgg actatttgtc tcctctgtcg tttggttagc aaaatttgac
    16081 gtttctgtat aaacgtttac ataaatactt gataagctcc ctaagaaagt aaaaatcaag
    16141 atgatcgaaa tcataatggg atgtaagtat tttcttttag atacagcttt gagtctagaa
    16201 acaaaatgtt tttgacatat acgttggatt ttattttgag ttttcttaat cattttcatc
    16261 cctcctctca ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt
    16321 attattttat tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat
    16381 tttagtcttg tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac
    16441 gattattgaa aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct
    16501 atgagggaag aataaaagag ttaggacggg attatctact atttttcttt gttttaaata
    16561 tttgttatct ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac
    16621 gaataaacgg tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt
    16681 tgctattccc cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca
    16741 ttggaataaa agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca
    16801 tctgtccaaa atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag
    16861 aattatcttt attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat
    16921 tttactgacg attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca
    16981 tattttgaat aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt
    17041 atgcggtgta gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact
    17101 tctaattttc aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc
    17161 caaatgtgat tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca
    17221 gtatcgaatt aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt
    17281 ctttcaatag gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat
    17341 tcatgaatgt gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct
    17401 ttctcctttt gattctaaga atactccatt tatgttagaa tattctggat tttttattaa
    17461 agcggttact cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg
    17521 tagatttatt ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg
    17581 gtgttcaaat tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc
    17641 tgataacaat gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga
    17701 tataaaagga tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt
    17761 tcaagaatcc tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag
    17821 caatcagagg ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt
    17881 ggtcagtatg gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc
    17941 ttgccattgt atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca
    18001 cgcaccgttt gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag
    18061 aatcaagata cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct
    18121 cacccaaagc cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc
    18181 gtctagcaga actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa
    18241 ctgccgtttg cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta
    18301 gagcaagcgc ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca
    18361 tcggtaattc ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa
    18421 gaaacttgtc ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt
    18481 ttggcattct cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt
    18541 taaaaatcat atttagaact tgaataagtg attgtaaact atttttggca tagccaacat
    18601 ttgttccatt aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg
    18661 tcaaatcaga caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc
    18721 gcttatcata ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct
    18781 cacctacaac actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt
    18841 tctccatttc cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg
    18901 ttcgatatgc ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc
    18961 gatctgtttt ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc
    19021 ctttggaaat ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact
    19081 tataaagatc gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc
    19141 cattctaaaa catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac
    19201 tcaccaagtt ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa
    19261 tcagtatttg ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct
    19321 tccagttttt tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta
    19381 acttccaact ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa
    19441 aattgtaaaa aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat
    19501 atgaaacaag gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct
    19561 aatgcaatag cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga
    19621 gagagagctg aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg
    19681 aaaaatatgc caaccaaaat cgaaatatgg aaatcctagc tcttccaaag ccatttgtcg
    19741 ttggtaccaa acactgccga gacttttccc aataatatcg ccaatttcac gatcattaag
    19801 accgatgaag aaggacataa aaatgatttc ttgtctttga ctaggtaatg ctgcaatcgc
    19861 atttgccaac tcctcattgg aaataaagat tttcatacca aggacaataa aagtaccact
    19921 actattcaaa tttggttcca aataatagaa ctctgtacct aacccttttt gttccaatat
    19981 ttccattgat atttgccgtt ggcgaaatct agcgtacttc ttttgaatat ttaacgcctc
    20041 attgcagact actttcttac aaaaactgtc aaacgtatgt tgaatgtggg tttcctctga
    20101 gtcgcccata atattctccc cctcaaaaaa ttcattcaac tcgttttact tctatccctt
    20161 tccattatta ctacaatcta gga
//
LOCUS       DACDKJ010000053        20163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDKJ010000053 DACDKJ010000000
VERSION     DACDKJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.053"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IV328_002050"
     CDS             <1..152
                     /locus_tag="IV328_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8938165.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IV328_002051"
     CDS             complement(230..763)
                     /locus_tag="IV328_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8938166.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IV328_002052"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IV328_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8938167.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IV328_002053"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IV328_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8938168.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IV328_002054"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IV328_002054"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8938169.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IV328_002055"
     CDS             complement(4488..5066)
                     /locus_tag="IV328_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8938170.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="IV328_002056"
     CDS             5388..5858
                     /locus_tag="IV328_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8938171.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="IV328_002057"
     CDS             complement(5966..7192)
                     /locus_tag="IV328_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8938172.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="IV328_002058"
     CDS             complement(7946..8146)
                     /locus_tag="IV328_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8938173.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="IV328_002059"
     CDS             complement(8280..8888)
                     /locus_tag="IV328_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8938174.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="IV328_002060"
     CDS             complement(8973..9443)
                     /locus_tag="IV328_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8938175.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="IV328_002061"
     CDS             complement(9491..10405)
                     /locus_tag="IV328_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8938176.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="IV328_002062"
     CDS             10551..11354
                     /locus_tag="IV328_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8938177.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="IV328_002063"
     CDS             11532..12479
                     /locus_tag="IV328_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8938178.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="IV328_002064"
     CDS             complement(12547..12840)
                     /locus_tag="IV328_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8938179.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="IV328_002065"
     CDS             complement(12868..13533)
                     /locus_tag="IV328_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8938180.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="IV328_002066"
     CDS             complement(13673..14305)
                     /locus_tag="IV328_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8938181.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="IV328_002067"
     CDS             complement(14312..15379)
                     /locus_tag="IV328_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8938182.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="IV328_002068"
     CDS             complement(15376..16107)
                     /locus_tag="IV328_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8938183.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IV328_002069"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IV328_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8938184.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IV328_002070"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IV328_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8938185.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="IV328_002071"
     CDS             18270..19439
                     /locus_tag="IV328_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8938186.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..>20163)
                     /locus_tag="IV328_002072"
     CDS             complement(19706..>20163)
                     /locus_tag="IV328_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938187.1"
                     /translation="KEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVT
                     EDGQVIYSSFSPNKDEIIFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFV
                     TVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6101 a         4021 c         3207 g         6834 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatataa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tca
//
LOCUS       DACDKJ010000054        19987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDKJ010000054 DACDKJ010000000
VERSION     DACDKJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.054"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..270
                     /locus_tag="IV328_002073"
     CDS             <1..270
                     /locus_tag="IV328_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8938188.1"
                     /translation="ELVKLEMAETGAGVRSSRHWKAAANIEFYYNEIQKGFDQMRELD
                     RQTNWSKKLHQDRFKFVEKYKEILEEYLRRTANDKKTRSIQHGFI"
     gene            230..475
                     /locus_tag="IV328_002074"
     CDS             230..475
                     /locus_tag="IV328_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938189.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            472..891
                     /locus_tag="IV328_002075"
     CDS             472..891
                     /locus_tag="IV328_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8938190.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            888..1445
                     /locus_tag="IV328_002076"
     CDS             888..1445
                     /locus_tag="IV328_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8938191.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1442..1738
                     /locus_tag="IV328_002077"
     CDS             1442..1738
                     /locus_tag="IV328_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938192.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1815..2228
                     /locus_tag="IV328_002078"
     CDS             1815..2228
                     /locus_tag="IV328_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8938193.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            2574..2948
                     /locus_tag="IV328_002079"
     CDS             2574..2948
                     /locus_tag="IV328_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938194.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            3178..3522
                     /locus_tag="IV328_002080"
     CDS             3178..3522
                     /locus_tag="IV328_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8938195.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            3527..3808
                     /locus_tag="IV328_002081"
     CDS             3527..3808
                     /locus_tag="IV328_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938196.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            3911..4225
                     /locus_tag="IV328_002082"
     CDS             3911..4225
                     /locus_tag="IV328_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8938197.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            4203..5897
                     /locus_tag="IV328_002083"
     CDS             4203..5897
                     /locus_tag="IV328_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8938198.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            5917..7095
                     /locus_tag="IV328_002084"
     CDS             5917..7095
                     /locus_tag="IV328_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8938199.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            7058..7744
                     /locus_tag="IV328_002085"
     CDS             7058..7744
                     /locus_tag="IV328_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8938200.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            7744..8904
                     /locus_tag="IV328_002086"
     CDS             7744..8904
                     /locus_tag="IV328_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8938201.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            8914..9789
                     /locus_tag="IV328_002087"
     CDS             8914..9789
                     /locus_tag="IV328_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938202.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            9786..10097
                     /locus_tag="IV328_002088"
     CDS             9786..10097
                     /locus_tag="IV328_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938203.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            10087..10440
                     /locus_tag="IV328_002089"
     CDS             10087..10440
                     /locus_tag="IV328_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8938204.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            10430..10831
                     /locus_tag="IV328_002090"
     CDS             10430..10831
                     /locus_tag="IV328_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938205.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            10824..11228
                     /locus_tag="IV328_002091"
     CDS             10824..11228
                     /locus_tag="IV328_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938206.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            11240..11848
                     /locus_tag="IV328_002092"
     CDS             11240..11848
                     /locus_tag="IV328_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8938207.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            11868..12230
                     /locus_tag="IV328_002093"
     CDS             11868..12230
                     /locus_tag="IV328_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938208.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            12233..12415
                     /locus_tag="IV328_002094"
     CDS             12233..12415
                     /locus_tag="IV328_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938209.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            12432..15851
                     /locus_tag="IV328_002095"
     CDS             12432..15851
                     /locus_tag="IV328_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8938210.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            15902..16639
                     /locus_tag="IV328_002096"
     CDS             15902..16639
                     /locus_tag="IV328_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8938211.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            16649..18940
                     /locus_tag="IV328_002097"
     CDS             16649..18940
                     /locus_tag="IV328_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938212.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            18964..>19987
                     /locus_tag="IV328_002098"
     CDS             18964..>19987
                     /locus_tag="IV328_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938213.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGG"
BASE COUNT         7134 a         2914 c         4256 g         5683 t
ORIGIN      
        1 gaattagtta agttagaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg
       61 aaagcagcag caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg
      121 cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt
      181 gtagagaagt ataaagaaat attagaagag tatttgagga ggacagcaaa tgataaaaaa
      241 actcgttcaa ttcagcatgg atttatatga tatcgaatca ggagcaacac tatctgtgga
      301 atcggaccat ctaatcataa attttggtgg aaagcgccag attattttgt gggtagttga
      361 tgatgtactg tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg ttgagtttga
      421 aatagtgaaa aaagtaatgg aattgattga aaaatacgag gaggacagcg aatgataccg
      481 aagtttagag cgtgggataa acgaaagaac gtaatgagag atgtagccgt cttgcatttt
      541 actaaaaacg gcaaaacaaa ctttattgaa tattggataa atcctaccga attgaaatca
      601 tatcacgtgc gaaacatcga cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg
      661 gatatatttg aaggggatat agtattagtc agcgtgcgaa atggcttcga ttacttagat
      721 aataaagtct gtattgtcaa aaattcaata gattattccg gattagtttg tgccactgtt
      781 gatgaagact tagagtatca aatttttaac acagagctgt ttgaagaata cacgtacgaa
      841 gtcaccggaa atatatacga gaatagcgag ttattggagg aacagcgatg aataaacagg
      901 aagcaatcaa aaagttagaa agtattaaag cgataggaaa tgatgcaata gctgcttgct
      961 ataacgagag tataaattca ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac
     1021 cagttattcc acaactcgtg gccggttggc ttgagaaatc tacggaccct tttacaaaag
     1081 ctgaaaaaat agcgtattta atcaaatcta aagatggtga ttcatattat ttctgtgatt
     1141 ggtttgtacg agatggcata gtgacgcaag agcaaggaga agaattactc gcttgggcaa
     1201 caagacaatc atatgaaaca ctattgagcc tatacaacgg ctacgaggtt gagaaagagc
     1261 cgttatgggc aataaagaat gccgatggaa actatcttac taaatgtgct ttatggggaa
     1321 aagatggagt aaattatagt tttgaatgca atccatctca tcgattgctt ttcactgata
     1381 aagcaacagc ggatgctgca gcattgttgg tgaatggaac agtggaagag gtggtagaaa
     1441 gatgaaacta aaagacggat tttacgctag cagtcacggt atcggcggtt taatgctaga
     1501 tatgccgaca aagaacccta aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt
     1561 ccgctgtgaa gcagaagagt tcgtttatcc gttcagagga tacgttaaaa agatactgtc
     1621 aaactcagca atcattcgta ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca
     1681 caagcattat ttagctgtag cgagattagt tgacatggaa gtaatcaaga gcaaataaaa
     1741 aaagccggat cgctccgact gattcaataa atccaacaca tttattatat cacataaagg
     1801 agcggtttga cttgatgcaa ttgttacgag aggtagattt caaacagaca agatgtaatg
     1861 cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc tctttgatag
     1921 atattaagtc gccgattatt acggatatgc cgaaggcacc aaagcacggc aataaggcag
     1981 aagacgcgat cattcagatg atggatatag aagcggagag agatgcgatt ctagcggctt
     2041 tgatggctct tagtctaatt agtcgccaga tactctacta cagcttctgt gtgccagata
     2101 gcttctcaaa ctacaggatt agccgtgaag tgggttattc agaaagaagt atacaacgga
     2161 tgaagtcgga agctctaata gagtttgcag aagcatataa acacggaaga ataattgctt
     2221 ataaataatt tggcggtttt ttggcggaat gatggcggtt tttagctatt taccagtgat
     2281 attatggtag tgtcgaaaga ttagtgatag gtctaagaca aaataaaatg taagggagga
     2341 aatctccctc atcgtttaat taagcttcga tagacagcag caaataaact aaaggatgtg
     2401 ggggtcagct cctacagtta gttcatgtgt tgctgtctat taattgcaac tctttcggtt
     2461 ttattgagta tttactcaac ttaaaaaaac tgttgtttgt agatgtaagc tgttcctatt
     2521 tgtacatagt aaaaaatgat ataataaaaa tattacgaaa ggggtgagag tgcatggaag
     2581 acaaaatgta tttaagacaa aaagttttca caaaaaaaga tattgaaaat caaattaatg
     2641 gatttataca gaaattagaa aatgcaaatt cttatttgat aaataaggaa ctaaataaag
     2701 cttatgacca gtggaaagaa gtatatgatg agttgaaatt gattcaaaat gaaacaaaat
     2761 tagtacgaac ggagaaaaag aatgaaaata gtttcttttt tgatggatat gctatattga
     2821 tgttggaaac tgtagcaaaa caaaatataa aagctcccaa aaaagaacta tctgacaata
     2881 ttgataacgc attagccgag ttaaggtatt acgttatgca aataaaagat gttaggatca
     2941 ctcgttgagt ggtcttttta ttttgcacaa aggaggtaac aacaatgtat agaccacaat
     3001 acttagaaca gaagtatgaa gtaatcactg tgcaaaatgg taacggagag atagtacgaa
     3061 agtatagaag accaataaag agcgatacat ataaacgaaa ggaaagcaat gaagttattc
     3121 cattgtatgg caaaagaata gctaagcatt aaataagatt gtgaaaggag acgggacatg
     3181 aacgaggaat tctatagatg gctattacag ttgataagag aagatcgttt ggttaagttc
     3241 tatcagtctc ctaaatggcg caggcttaga gagaaagcga tgaaacgaga tcactatgaa
     3301 tgccaagagt gtagaagact aggtaagtat catagagtag agaacgttca tcatataaag
     3361 gaagtcaagg atagacctga cttagcttta gatttagata atcttatttg tttatgtgtt
     3421 gaacatcata atgaagttca tggcagatat cttacagcat tggataaaca agagaagaag
     3481 atagaaagct ttgctaactt cgatgcaagt gaaaggtggt aagtgcatga tcatcaatga
     3541 taatggcaga gagtatgata cagaaaagat tgaagagtat tcatcttata ctcagggatt
     3601 aattaaacgt ttgatatacg ttcgctatgt aggtattagg gatctgttat cagataactg
     3661 ttgtagtaaa tacaaagtga atcaagtaag agaagcgttg aataaagata ataacgtcga
     3721 aagaataaaa aatgtttttg gatatagtat tgaagagatt aattattaca ttgacttcgc
     3781 tgaagctttc attccgatgg tgagataacc cccccttaaa ataaatcgca aattttttgg
     3841 gggtgatgaa acggaggggg ctgtcaggaa aagagatttt ttcgaacttt atcatgaaag
     3901 gagggctaaa atgtttaaaa acgaattgtc tcaaaatcgc tacagagaaa aattacgccg
     3961 ctctttaata agccaattgg aaagtcagaa aacaaatatt gagccattct tagataatgt
     4021 tgatcgttat atcagtttat gggaaacggc gatatcactg gaagaagata tatccgagaa
     4081 cggcattaga ctggagaatg gtaaaaagaa tgaatcagta gcgttgcttg tttctgtcaa
     4141 caaacaaatg ggattgatgt tggataaact tgccattact cctgaattgg taggtgaagc
     4201 aaatgaatca attcctgagt tataagcata ttgaaaattg gttcaaagct atagaagaag
     4261 gcactatcaa ggtatgcaaa gagcaattat tgctaaaaaa ttatctagaa gaaagagtct
     4321 ttactagaga agatatttac ttcgataagc agatggtaga ggattcaatc aatataccag
     4381 cacaatactt tccattcgaa ttaattccgt gggaaaaatt tctacaatgt tttatttatg
     4441 gtgttcgatg gaaaaaagat aaaacactag tgttcaatag atatctttca ttaatgggac
     4501 gtggtaatgg taaaactggt tttgcttctt ggaacaactt ctttctacta accgctaaac
     4561 acggtattaa aaattatgat attgatatct atgccaataa tgaaagccaa gcaaagacta
     4621 gttttgatga tgtatttaaa gtaattaaag atcatcctga tttagataaa aaagtattta
     4681 aagctacgaa ggaagttatt caaaatatcg ctacaaacag caaacttcgt tataacacgg
     4741 caaatgctag aacaaaagat gggaagcgac caggtgcaaa ccgctttgat gaaattcacg
     4801 aaaatgaaga ttattcaatg ataaatgtgg ctacttctgg tggtggtaaa attcgagatt
     4861 atagagaatt ttatgataca actaatggtc atgttcgtgg tggtccgctt gatgacatta
     4921 tagaagaatc aaaaatgatt ctttctggag aacttggaat tgacaaggat ggagcagaat
     4981 tttctagttt gtttccattt atttgtcgct tggataacga taatgaagtt gatgatcccg
     5041 acatgtggga aaaagcttgt ccaactatta attacaatgc aaatttaaaa cggaaaatgt
     5101 ttcaagaata ctctcaaatg caacgtaatg ctggtttaag acttacgttc atgaccaaac
     5161 gaatgaacag acctatggaa gatacacgat ttgctgttgc ttcatatgat gatgttctgc
     5221 atacgaaaga aaaagaattt cctgaaaaaa tggatgaagt gataggaaca gtcgattttg
     5281 ctgatagacg agattttgcc agcgttgggt tgctaggaaa atacgataaa gatgtttatt
     5341 ttacacaaca tacttttatc cacgaatcag cccttcgatt acaaaacatc aaacgagagg
     5401 ttatagatat ttctatagat caaggaaaat cacagatcgt tcatggaaaa aatatagaag
     5461 ctgattatat tgtaggttgg tttcttgaaa tgagtaataa atattatatt aaaaaaatcg
     5521 ctatggatat gtaccgtgca aaaatattga agcccgcttt agaagaagca ggttttactg
     5581 tggaaattgt tcgaagcgga tctgttacac atggtatgtt aaaagatctg gttgatgacc
     5641 tttttattaa tcaacgttta ttttttggtg acgatgcgat tatgcgttgg tattgcatga
     5701 atgtatatga agagcatatt tctaatggaa atatacgcta tgaaaaaata gaacctgaaa
     5761 ctagaaaaac ggatggcttt ttttcattcc ttcatggttt gaatttttta gatgatattt
     5821 atgattctgc tcctgtaaca gtcacaaata gctcagtaga aaatacagga actggattta
     5881 ctcctctagt attctaactt gaaaggaggt gagaaagtgg ggatttttca aaaggcggta
     5941 ggatacttca caaaaaaagc aacggttcct ttagaagaat acttttgtaa attgcaagtt
     6001 gattttgtgt atcgaaaatt tgcgattgaa acttgtattg atttgattgc aaatgcgatg
     6061 agtaaagcgg aattcaagtc atatgaagat ggaaaaaata aaaagaatga tctttactat
     6121 aggctgaatg tagctcctaa taagaaaaat aatgcaacag aatttagaaa aaaactgatc
     6181 aggagattaa tattctacaa tgaagtattg atcgtttctc cgtctaataa ttctagcgaa
     6241 atatttattg cggatagttg ggatgtcaca gaatatgcat tgaaagatga tgtgttttct
     6301 caagtgcaaa ttaacaacat agtccttgat agagaatttc tagaaagtga tgttatctat
     6361 ataaaatacg cagatcaaca aattaggcaa ctagtcgatg cgtattatca agcgtatggg
     6421 aaactcattt ctagtgccat gaatgtttac aagcgctcta acgctcgtag atacgtactg
     6481 aaagggaatt tattccgacc gcaagacaat acaacacaag atcaaatcaa taaaatgatg
     6541 acatcacaat ttaaggcttt tatggaagct gataatgcag gtgcggtatt tcaattacaa
     6601 aatgagtaca cattagaaga tttcagcgga aacttccaaa gcaattcaag agatataaaa
     6661 aacttaatag acgacatctt tgagatgaca gcagcagcgt ttcacgttcc gaaaaaccta
     6721 ctaaagggag acatgagtgg gttatcggat caagtggacg cttttttaat gttcgaaatc
     6781 ataccgattg ctgaacttat tcaggatgcg tttaacgcta gtctctatga agtagaagaa
     6841 tacttgtcag ggaattttgt acgtgtggat acaactatga tcaagattac tagcttcaaa
     6901 gatttggttg acgctattga tgtaggcatt agaaatggag tatttacaat caacgaagga
     6961 agagaacgcg ttggaaatga tcgctctgat aaggcgatgg cagatgaaat atttataact
     7021 aaaaacaatc aacaagtatc gaaaggaggt gaggcgaatg acgacaatga aaacatttct
     7081 agcagtaaag aatgaaggcg cagtaccgca aatttttatt cagggattta ttggttctag
     7141 ttggttcttt gaagggaata ctgacaaggg aatcaaaaat attttggata gtctaggtga
     7201 tcaagaagaa attgaagtag taattaattc aaacggtgga gacgtatttc aagggattgc
     7261 tattgggaac ttacttaagt caaataaagc aaaagttaac gttgtgatta acggcttagc
     7321 cgctagtgct gcttcaatta tcgcaatggc tggcgatact ataaaaatct acaacaatgc
     7381 acaattgatg attcaccgcg cttccacata cggagaagga aatgtcgatg acttccgtac
     7441 gattgctgac caactggaat caattgataa atcggtaaag gcttcatata aaacacgatt
     7501 caatggcaca gatgaagcat tgcaagaact tcttgaaaaa gaatcgttta tggatgcaga
     7561 aacagctttg agttatggat tggtcgatga aattatcgat gcagaaaata gctcaggtac
     7621 tgaagctaaa aaagaacaaa gcgttgaaga aattttgaat gacgttaaag aaaaaagagc
     7681 agaaaaaatt gctgcattta cagcagcatt aaataaaaca tttggacaag gagatgtaaa
     7741 ataatgacag ttaaaaattt aaaaggtgta acagctgcaa gcgaccaatt gatgaaagct
     7801 tttaaagatg gtaacgaaga atcttttagc gcagctatgg taagcttatc taaggaaatt
     7861 caggataaaa ttttagaaga agcaacagca aaaaatcaag atcaattagt attaatgaac
     7921 cgtggtcagc gtgtattaac tacacaagaa acaaaattct ataacgaagt agtgaaaaac
     7981 gaaggttttg caggggtcga agaattagtg ccagctactg tatttgaacg tgtatttgaa
     8041 gatttagaac aatctcatcc actattgcaa aaaattactt ttgttaacac aactggtgta
     8101 acagaatgga ttgtgtcacg tggagtcaat ccagcatggt ggggtaaact ttgcgaagct
     8161 gttaaaaaag ttttagataa tggctttgat gtaattaaca tgaagcagtt caagctatca
     8221 ggttatattc ctgtatgtaa ggcaatgctt gatttaggtc cagtatggtt agatcgttat
     8281 gtccgtactg ttttagtaga atcattgaga attgcattag aacaagcaat tgttgatggt
     8341 actggtaaag atatgccagt cggaatgatg cgtgacatga gcaaacaaac tagcggagaa
     8401 tatgctgaaa aaaaagcaga acctattaca gctttagatg ctgtaactat gggccgtttg
     8461 atggcgcgac tatcaaaatt caatatcgaa ggtgtgaatg atccgattta tcgtaatgtg
     8521 aatccttctg atgtggtcct aattgtgaat ccaactgatt actggtctaa agtattccca
     8581 gctaagactg tactaactgc taatggagaa tacgtacaag tattgccagt accagtttca
     8641 gatttgcagt caacggctgt gccagaagga aaagcagtta ttggggtagc ctcagattac
     8701 ttcatgggtg taggatctac actaaaaatt gaagcttcag atgaatacca ttttgttgaa
     8761 gacgaacgca tttatctagc taaacaatat gcaaacggtc aacctaaacg taacgatagt
     8821 ttcattgtat tagatattag cgctttggga actactacta caactacaaa accaacaacc
     8881 acaacaacta caacacaagc gtaggtgatc agaatgaagt atattctttg tcagccggca
     8941 atcaatcggt ttaaatggga gcttgaagtt tgtttaacta atctgaagaa actaggaatc
     9001 aaagatatcg tattgctttt cagcagacac gatgatcaga ttcctatttt ttttgagaag
     9061 gaatatggtg ttgaagttca tgtgtacgat gatctgcggg acgacaaaga gtatattcct
     9121 tcgattaaac catatttatg gtggaaatat ttagaagaag atcattcgcg tgaggacgac
     9181 cgatatttct atatcgattc ggatgtcatt ttcaataaaa gaattaattt gcgcaaattg
     9241 ccttctaaag atgatgtttg gtattgtagc gactgctgta gttatctaag tcttgattat
     9301 attagaagct gtgaaaacgg agaaaatatt ctaaaagata tggcaaacat tgtaaatgtt
     9361 acagtagaat ctttggaaac tataaacact aattcaggag gcgcacagtg ggttattaac
     9421 cgtcctaaag cgaattattg gaaaaaggtt tatctggatt ctaatcggct atatcgctac
     9481 cttagagggc aaaaaacaaa tatacaaatc tggacagccg agatgtgggc acggctttgg
     9541 aacatgatgt atttcaatat tggtcctaaa gttcacgagg aattagactt ttgttttgct
     9601 actgatccaa tagaaaaagt taaagaagta aaaatcttac acaatgctgg agtaacaaca
     9661 aacgatgaag atttattttt caaagggaga tacgtgactt ctacgccttt tgatgaagat
     9721 ttatcatttg taaacaagaa aaaatgctct tacgcatatg ttaaagcaat taaggcggtg
     9781 gttagatgac gcctgaacaa gtgactgaag aattgctaat agctgtgaag gataatattt
     9841 acgttacctg gaacgaagaa gatgagtcaa ttaaaaagat gatagctaaa aatgctgttt
     9901 atcttcaaag taaagtgagt acaacacttt ctttttctcc tgaaagctta gaatacggat
     9961 tgctaatcga aagatgtaga tacgactgga atcgtgcttt agatgagttt gaacaaaatt
    10021 tcgctagtga gttattaggt ttcattcaac attatgcgct acaagaatat attgcaggtg
    10081 atgtgaatgg cgaataatcg tagactcgaa gaaacattca acgatggttg gttaaagatt
    10141 ttgacgcaaa ctaccaaaag aaatgaacta ggaaaaaaga ttggtgtaga agatacagaa
    10201 atcacttctt taaaatttag aaatctttcc atgagagata gtgatataac agctatggat
    10261 gcgatgggat cgaaattaac taagaaagta aagactccat ttcatccaat cgccaagaaa
    10321 tttaataaag atcaatattt tatcgtaatc gatagtatgc gttacaacgt tatctatgcc
    10381 gattacgata atttttatat ctatttttat cttgaaagtg tgggtgaata tggtgattga
    10441 taattctaaa gaaaaagaac gtttaaataa gcaaatttct gctatcaaaa cttccttaga
    10501 agagcatttt ggcctcaaac tctttcaaga ctccgtaggc gaggatgagc tacctgatga
    10561 ttttaattac ttcattctcg aaacaggaga aatagaaatg atcactgagc caaaatatag
    10621 cgtgggtcaa aatctatatc taactttcta ttcagaaaat agagaagatt taacaggaga
    10681 ctcactagat attatttcat tgattcaaaa tcgttcgatt cgttttcaga gaatggatcc
    10741 caatcattta aaactagaga accaagatcg ctatatcgat caattggtat ttacgtttag
    10801 acgattattg aagagtgatt gtcatggcta aaaatagttg ggagctaaaa ataaatggac
    10861 atgatgaact tcttgtgcgg atggaacgct attcaagcga gagcgaacga ctgattaacg
    10921 aagcattgaa atcaaaaggt tcggctattg cagtggatag gattacagaa aaaattcctg
    10981 tttctgaagc agatttaaga agagggcacc aacacgcaaa aaatagtcgt ccacttaaga
    11041 ctcaatacat taatttgggt ttcatcatta gacctacaag aaaatttgag tatttaaaat
    11101 atcctgattt ggggataggt acttctaaaa gaaatcagcc ggacgaattt atgagaagag
    11161 gattaggtct tgcacttgat ccaattacag aacttctgat tcgtcaattc gataaattaa
    11221 ataaataggg ggaacaacaa tggctaaaac aaaaactgta gtaacaacgt tcgataacgt
    11281 aagtatcaaa cgaattgctt ttaattttaa gaacgcagga aatgcaatcg caacgaattg
    11341 taatggacaa ttagatggcg aaacagaaat gcaaacggtg gttaaaaaat gtggagcgac
    11401 agaagtaaaa tcaaaatcta aaccaatcaa tatgacggta acaattactg cacatgtacc
    11461 gatggaagtt tatcgacgtt tcaatgggtt gaaacaagat ggacgtatta aaccaggcat
    11521 ttactcttac ggtcctgatt ccgtaggcga agatttctca cttgctgcag agatcgtgga
    11581 tgacttcgaa gaaaatagaa agttagttgg tatgttagca tgcacttcga atacaggatt
    11641 aacattctct attgaaaatg gtgcggatga agtagctgcg ttagaactag aaacaaaagt
    11701 tatgcaagat gaatttggta aattttatca tgaagcaatt gttgcagaac ttgaagaaga
    11761 cttaacagat caatggatga cgaatctatc tgctgatgtg attaaaaaaa cttcagttgt
    11821 gacaactacg gccactcaat cacagtaaaa aaaacggagg tagcgaaatg aacgaagatt
    11881 actcaaaaat tgaactaaac gatggaacaa ttttgaattt agaacctaaa ctgaatatca
    11941 agaaattatt gatgatcaat agagatttta acacagacga gtttgcaaaa atgactgtgg
    12001 gaaaaggatc catggatatt tctgttattc aaggtgcaaa ggctgtgtat attgcttacc
    12061 gccaagcgaa catgactgat tatatttcat tcgatgaatt tatcgataaa tgggattttg
    12121 atatggctac tgccagctat atttatcaat tgatgatgtt caaacaagca cgcgatgctt
    12181 atcaaaaaga atttgaaaaa gcaaataagg aaaaaaagct tcaaaagtaa aaatgccaaa
    12241 gctcttagtt gaaacgtggg tcgatgtcta ttcgatgttg accgacgttt tttctatgcc
    12301 ttcagatttg gttttaagcg atatctgttt agatgacatt ttgcaaatgg cttacaacaa
    12361 gagtgcttat gaaggatgga aaaactatgc aataaaccaa tctcagaaaa actaaagaaa
    12421 ggaggtaaaa aatggctaaa aagagaacag aagcagaagt aactttcata gctaacgatg
    12481 acggattgaa atctacgtta aaagaaatca gtgctgaatt aactaaaaat agagcagaat
    12541 taaaactaga acaagctcaa ttacaacaga ctggttctga atcagacaag ttaggaagta
    12601 aattatcttc tttagaaaag cagtatgaat tacaaagcca aaaagttgaa gtaactagcc
    12661 aacgtttagc caatgccaaa aaatattatg gagaaaattc caccgaagtt cagaaacttg
    12721 agagagaact gattaaccaa caaacagcgc aacaacgttt gtcaaacgaa attgataaaa
    12781 cgagtaatac actagctcaa gcaaaaggcg aaatacagac gtacgagtct acaatgcaac
    12841 agttggatag tgaacaaaaa aatgttcaag ctagtgcttc tctgattgaa tccgaataca
    12901 aaaaatggca agtaactgct ggtcaatcag cttctgaatc cgagaaatta gcgaaagccc
    12961 aagaatatgt ttctcaacaa tctgaaaatg cagagaaaac gatagatatc ctgagacgac
    13021 agttagaagc tacacagtct gagtttggcg ctacatccac agaagcaatg cagatggagg
    13081 cgaagcttaa tgatgctgaa cgtgaatttg aagagttagg acaagctgct aaaaatgtag
    13141 atacaactaa cttggacgat atcggaagca aaatagatat gaataattta atggaagctt
    13201 ctgacgtttt aagcgacatt ggcgataagc ttacagaatt agggaaacaa gcagtggact
    13261 ctgctaacag tgtaggtagt tcccagagta aaatacaagc taattttggt ttgactaaac
    13321 aagaggctga agaattaacg aatgtagcca gagacattta ttataaaggt tttggagaat
    13381 cgttagatca gtccacagat gcattgattt tggtaaagcg taatttaggc gatttaaata
    13441 atcaagattt acaaaatatt acggaacaag ctatggtcct agaaaacacc atgggcgctg
    13501 atatggatga aacgttacgt ggtgtaaatg gcttaatggt caacttcgga ttgagcgctc
    13561 aagatgcaat ggatttaatg gtttcgggta ctcaaaacgg tttagataaa acgcacgaat
    13621 taggcgacaa tatggcagaa tatagccaat tatggagtca aatgggatat tcagctgatg
    13681 aaacgttcgg aatgcttcaa aatggtttag atgcgggtgc ttataacctt gataaagtca
    13741 atgacttagt taaggaaatg ggaatatcgt taacagatgg tcgatttgag caaaacatgg
    13801 atatgtttag tgaaagtact agaaaagctt ttgaagagtg gaaaaatggt ggaggaacac
    13861 aaaaagacgt tattaattcc atgattcaag attttagcaa tatggatggt caatacgacc
    13921 aattaaataa agcttctaca atttggtctg cacttggcga agataacgcg atgaaagttg
    13981 tccaatcttt gactgatgtt aaccatacat ttgatgatgt tagtggatct gcacaaaaaa
    14041 tgaatgaaga ttctactact ccgttacaag aattaaacgg aaaaatagct gaattaaagg
    14101 attcattagc tcctataggc aacacaatca tagatgcact cgaaccagta attgattttc
    14161 taggaaagat ggctgatgcg tttaataatc ttccacaacc agtacaggat tatgccgtag
    14221 cgattggcgg attgactgct gcatttactt tattaatgcc aataatagtt ggcttcatgg
    14281 ctctaggtgg tcctactaca ttaataatag gagcagttat tactgctatt gctggagtta
    14341 tagcaattat aaaaaactgg ggcgcaatta ctgactggtt taagggaata tggagtaaat
    14401 tcactgattg gttgggtggt acttgggaaa gcataaaaga aggtgcctca tcagtttggg
    14461 atggagtcaa agaaacctgg tctggatttg tagattgggt tcaagaaatt tggcaaggag
    14521 tttctgattg gtttggagag ttatggagcg gattagttga aggagcttcc aacatctggc
    14581 aaggagtcca agagacttgg caagcattcg ttgattgggt ttcaaatatt tggaacggag
    14641 tcaaagaagt atggtcgatt atttgggcag acattgtagg aattgttcaa ataccatgga
    14701 cattaataac gtcattgatt caagctggta ttaatattat cgtgggtatc tttgatgtag
    14761 ctggacagtt attaggcgca gcttggcaag ctgtttggac acctatttct gatttcctta
    14821 aaaatacttg ggatactatg acacaatgga taagcatcgc ttggaatgga attgtaacta
    14881 cattccatac tatatttgat ccagtagtgg catggtggaa tggtatatgg acatctatta
    14941 gtactacggc ttcaaatatt tggaactcaa ttagtgcaac agcttctagt atttggaata
    15001 gtatcaagaa tacaatcact agcttggtac aagcagctgc tacagtaatt caaaatattt
    15061 ggtcaactgt atctagttgg ttaggtggaa tttggaattc aatcagctct acagcatcaa
    15121 atatctggag tagcgtgact agtagtataa gcaatgctat aaacgcagct aaaagtgcca
    15181 ttcaaagtgt ttggaatagt atatcttcgt ggatcagcgg aatttggaac ggtatcaaaa
    15241 acactgcttt gaatctttgg aatggaatta caagcactat tagctctaaa gtaaacgatg
    15301 gaaaaaatgc aatttcaagc ggttggtcca atctaacagg tattgtttcc gacatattca
    15361 ataatgttaa aagcacgata gctaatatat gggaaggtat caaaaagact gttagcgctc
    15421 cgattgattg gattagagat aaaatcagta gtgtctttga taatttgaat atttctatac
    15481 cacatattcc gttaccacat tttaaattga gcggagaatt caatccattg aaggggaaaa
    15541 tcccaacatt gggtgttgat tggtatgcga aaggtagtgt gtttaattct ccaaatatta
    15601 tcggtgtagg tgaatcagga cctgaagcag ttttaccttt gaaaagatct gtgctgcaag
    15661 aaattggtga tcgtatcttg agtagcacct cagtttcatc taggacacaa gcggttcaac
    15721 ctgtgaacaa ttacgaattc aatttcacaa ttgatggtaa cgcagatgaa gtcactatga
    15781 agcaaacaac tcaacaaatc attgatagca ttacaaaagt tcaaaatgat aatgcttcgg
    15841 catggcgtta aacaggagag tatttctcct gtttttttag tattaaaaag gatgtgaaaa
    15901 aatgactgat tgtatacatt ctataatcga tggatttcct gattatttgc ataaattggc
    15961 tttagctgaa agaccaacca taccttctcc aaaaagacag agagttgaaa cttctgtttt
    16021 aggaaggtta ggtggcttag tgcaagatta ctcgtttgaa gacatgtcgt ttacattgca
    16081 ctataactat ttagaggatg tggaagacca tcaagcgttc aagcaatcgt tttatatcat
    16141 gcgtcattgg ttaaactatg caaagaaatt agaattctct gacgatccca acgtctatta
    16201 cgttatccag actatcgata ttggggatgc agaaaacgat attgttgaat ggggagagtt
    16261 cgatgtaaat attactgcga aaccattcgc aagagttcaa gaagatgtac ctataaccgt
    16321 agataaacca cagtcattta acttgctgaa taatagttta gaagaaagtt ttccaaagat
    16381 tatcatcact ccttcagcta cttcatgcca gttcatctta aatgattatg tgtttagttt
    16441 cgaaggctta gtagcaggaa ctgatgtagt cattgatagt gatttgatgc tttgctacga
    16501 agagcaatcg gacggagata ttttagatcg gtccaacaaa atgaagacca tgcaatatcc
    16561 gacattgcaa gtggatatca atcattttaa ttgcactggt ttgagcaaaa tacaaattta
    16621 tcgtaacggg ttaaggtagg tgaaatagat gatcgataat ttaataacta tttacgataa
    16681 aaatgacgcg aataatttag ctgaacattt atatgatacg caaggtttag gcgctttgtc
    16741 agactggtta acagctactg ttagcaataa actaaacgga gccgagatat ttcagggtac
    16801 ttatccaata agcggaacta atgcagattt gattgtagaa ggacgtatta ttcagtgtta
    16861 tgtagatgaa aatcgagcaa aacagcgtct acggatttat tatgcaaaaa cttctgtaat
    16921 aggcaatacg atagaagtaa aagctgaacc tattttcaat gatataagaa aatcggtgtt
    16981 gaataaatat gacagcggaa cagaaaaaat cactgctact caggcatggc aaaacgcaaa
    17041 agctttagcg aaaccagcta tcccttcgca gttttctttc tcgtcattag tagatacgct
    17101 tgctaatgtg aagatagaaa aagcgaattt tttagaattc tttggtggaa aagagggatc
    17161 tattctagat cgatttcatg gggaatttct taaagataat aacacattac atcatgaaaa
    17221 aaggctaggt acggatcata aaatcaaagc gatttatact aaaaatttaa ctggtcttga
    17281 cttagagata gatgctcaaa gcgttttagt tggagtttat ccattcatta gcagttcttc
    17341 agaaggagaa gacgagatca ctctaccaga agaagtcatt ttcacggatt acgtggatga
    17401 ttatcctgct ggatatgttt cttttgttga ttttaaagac aaagcgactg atgtagccac
    17461 attaagggaa gctgctaaag actggttgaa aacaaacata gataaacaaa aaccacaagt
    17521 gagtggttcg attgaattag taccattgag gcatcaaaga ggctatgaaa aatttgttga
    17581 tctagaaaaa gtttcgatgg gtgacggagt agatgtgtat catccacagt taaaagtgaa
    17641 tatgtcagcg agaattgtgg aatatacgtt taatgttcta accaattcat acgataaatt
    17701 agttgtagga aacgtcaaaa caaacttctt agaaaacaca gagaataatg taagcaattt
    17761 gattaatgat gccattgatc aattgaaaaa tggtggcgaa atcagtgatt taatcaatga
    17821 tattgtagat catcaaactg atatgattac tggtcaaaat ggtggttatg ttttattaga
    17881 tcctaaagaa gcgcctagtc gtattttgat tatggacaca ccagataaga ataccgcaag
    17941 gaatgtttta caaatcaaca atgctggtat tggtttttct aaaactggca ttaatggaac
    18001 atatgaaacc gcatggacat tagatggcgg attcaatgcc tcgtttatta cggctggtga
    18061 aatagtagga attactatta gaggtactac attaattagt gatggcactg attatagaac
    18121 aagtattgct aatggcaaaa tgacttggta ctcaaaaaaa gttaacaaag atattatgga
    18181 gctagaagca cgtgattatg taagtgctga tgccggtatt gtatcataca ccatgaaaac
    18241 tggtggtggt ttcatgatta gaaatccaca gggtaacttg gtttttagta cgtgggataa
    18301 tggtaataac agaccgtttc tatcttttgg tgcgcccaat ttcaggtata gcaatgctag
    18361 ttatgtaact tctggcgacg gtagttcttt aagcattaat ggtagtgcgg gtaactcatg
    18421 ggaatttaag gtagctggta ggactatgaa atttactagt gatggtatgc taacgttgcc
    18481 aggttgtttt tttggttcat gggaagatgg gaaacttgct aggtttgaac aatcaacggt
    18541 acaagtatat aaagatttta ctgttagagg tactaaaaac tcaaccgtac caacagaaca
    18601 ttatggacaa cgactattaa acgcttatga aactccagaa tattatttcg ctgattatgg
    18661 ggaagccgtt acaggtgatg atggtaaagt tcgtgttgat attgacccca tgtttgctga
    18721 gacagtaaat ctaagtcgat atatgacaca tgtgacacct acagaactag ttttgtgtgc
    18781 tgttactcat gaagatattg accatttcat cattgaaact agtaagccaa acgtattagt
    18841 tagatggaat ttagtggcac accgtctagg gtatgaagat attagattaa aagaggatac
    18901 agcatatgat agcacagtgc ttgaccaaaa acgtttttaa aacgaagaca aggaggtata
    18961 taaatggcta gcagtttata taatttggct ctagatttca gcaaagaatt aaactacacc
    19021 aaagctatta tggctcgtca aggtgataaa gggattacag tgactgttaa accattttta
    19081 aatggcttgc agatggatac gagtggcgga acatttactt taaaaggaac aacgccatct
    19141 aaccgttacg tagatagtgt ggcaactagt gtaactagtg aagaagtcac gttttctctt
    19201 gatggcacat ttatgagtga agcaggatat tataaacact gctacgtaga atatagaaaa
    19261 gacaatcaaa ttttaacaac gcaagatatc atttttttct cactaggagt gtctgacatt
    19321 tcgcaaggcc aagccgatga atacgtttcg caattggaag agttgattca aaagtataac
    19381 gaaacttttg atgcttttat ggctgaaatc gaaggtagag tggacagctt aaataaacag
    19441 attactgatt taactggtca agctaaaaca ctacaagaca agttagacgc tctgaaagaa
    19501 gaaatttcta aattaggtaa cttgcaggtg atgtacagta acagcatcga cttcggggga
    19561 tatgattata gcggtaagcc gaatttaatg tccaaactaa aatcgagcga ttttaacgtt
    19621 ggttaccacg ggtcactaac ttcggataac gaaaagctac attttacttc tgatggtaca
    19681 ggaagcatta ttatgtttac gcgtattaat acacctcagc ttgctagtgg gaaaacctat
    19741 actctgagtg cgaaagttcg atttgatgaa ggaactacag gagctattga taaattacgt
    19801 ttggtgtatc gtacatcacc aggagaaaag atattattgg aagcaaatag tacaaatatt
    19861 acaacagatg atgtagggaa agaaataaca atcaaaggta cagctaacgt taattatcaa
    19921 atcacaaatt tagatcgatt ttatatgagt attagctttg ttgacaggga taaaataaat
    19981 ggcggat
//
LOCUS       DACDKJ010000055        19821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDKJ010000055 DACDKJ010000000
VERSION     DACDKJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.055"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV328_002099"
     CDS             6..305
                     /locus_tag="IV328_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8938214.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV328_002100"
     CDS             378..1739
                     /locus_tag="IV328_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8938215.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV328_002101"
     CDS             1761..2021
                     /locus_tag="IV328_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938216.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV328_002102"
     CDS             2035..2787
                     /locus_tag="IV328_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8938217.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV328_002103"
     CDS             complement(2881..3486)
                     /locus_tag="IV328_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8938218.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IV328_002104"
     CDS             complement(3539..4513)
                     /locus_tag="IV328_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8938219.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IV328_002105"
     CDS             complement(4891..5670)
                     /locus_tag="IV328_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8938220.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV328_002106"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV328_002106"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8938221.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IV328_002107"
     CDS             7161..8558
                     /locus_tag="IV328_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8938222.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IV328_002108"
     CDS             8639..9079
                     /locus_tag="IV328_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8938223.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IV328_002109"
     CDS             9227..9553
                     /locus_tag="IV328_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8938224.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IV328_002110"
     CDS             9554..10303
                     /locus_tag="IV328_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938225.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IV328_002111"
     CDS             complement(10383..10838)
                     /locus_tag="IV328_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8938226.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IV328_002112"
     CDS             complement(11265..11540)
                     /locus_tag="IV328_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938227.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IV328_002113"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IV328_002113"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8938228.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IV328_002114"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IV328_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8938229.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IV328_002115"
     CDS             14770..17436
                     /locus_tag="IV328_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8938230.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IV328_002116"
     CDS             17800..18552
                     /locus_tag="IV328_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8938231.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IV328_002117"
     CDS             complement(18574..19728)
                     /locus_tag="IV328_002117"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8938232.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6330 a         3473 c         4060 g         5958 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DACDKJ010000056        19475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDKJ010000056 DACDKJ010000000
VERSION     DACDKJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.056"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IV328_002118"
     CDS             complement(21..995)
                     /locus_tag="IV328_002118"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8938233.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="IV328_002119"
     CDS             complement(1075..1950)
                     /locus_tag="IV328_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8938234.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="IV328_002120"
     CDS             2080..3987
                     /locus_tag="IV328_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8938235.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="IV328_002121"
     CDS             complement(4528..7806)
                     /locus_tag="IV328_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8938236.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="IV328_002122"
     CDS             complement(8077..8622)
                     /locus_tag="IV328_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8938237.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="IV328_002123"
     CDS             complement(8634..9326)
                     /locus_tag="IV328_002123"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8938238.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="IV328_002124"
     CDS             complement(9345..9629)
                     /locus_tag="IV328_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938239.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="IV328_002125"
     CDS             complement(9642..10205)
                     /locus_tag="IV328_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8938240.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="IV328_002126"
     CDS             10433..11095
                     /locus_tag="IV328_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8938241.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="IV328_002127"
     CDS             11219..12415
                     /locus_tag="IV328_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8938242.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="IV328_002128"
     CDS             complement(12516..13892)
                     /locus_tag="IV328_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8938243.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="IV328_002129"
     CDS             complement(13929..14795)
                     /locus_tag="IV328_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8938244.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="IV328_002130"
     CDS             complement(14884..15909)
                     /locus_tag="IV328_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8938245.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="IV328_002131"
     CDS             complement(15927..16601)
                     /locus_tag="IV328_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8938246.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="IV328_002132"
     CDS             complement(16785..17678)
                     /locus_tag="IV328_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8938247.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="IV328_002133"
     CDS             complement(17752..19449)
                     /locus_tag="IV328_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8938248.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DACDKJ010000057        19074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDKJ010000057 DACDKJ010000000
VERSION     DACDKJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.057"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..230)
                     /locus_tag="IV328_002134"
                     /pseudo
     CDS             complement(1..230)
                     /locus_tag="IV328_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(484..2119)
                     /locus_tag="IV328_002135"
                     /pseudo
     CDS             complement(484..2119)
                     /locus_tag="IV328_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2674..3237)
                     /locus_tag="IV328_002136"
     CDS             complement(2674..3237)
                     /locus_tag="IV328_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8938249.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(3252..4295)
                     /locus_tag="IV328_002137"
     CDS             complement(3252..4295)
                     /locus_tag="IV328_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938250.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(4428..4832)
                     /locus_tag="IV328_002138"
     CDS             complement(4428..4832)
                     /locus_tag="IV328_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8938251.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(5065..5700)
                     /locus_tag="IV328_002139"
     CDS             complement(5065..5700)
                     /locus_tag="IV328_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8938252.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5966..7018)
                     /locus_tag="IV328_002140"
     CDS             complement(5966..7018)
                     /locus_tag="IV328_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8938253.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(7031..7969)
                     /locus_tag="IV328_002141"
     CDS             complement(7031..7969)
                     /locus_tag="IV328_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8938254.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(8420..9070)
                     /locus_tag="IV328_002142"
     CDS             complement(8420..9070)
                     /locus_tag="IV328_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938255.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(9170..9679)
                     /locus_tag="IV328_002143"
     CDS             complement(9170..9679)
                     /locus_tag="IV328_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8938256.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9880..10749)
                     /locus_tag="IV328_002144"
     CDS             complement(9880..10749)
                     /locus_tag="IV328_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8938257.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(10769..11890)
                     /locus_tag="IV328_002145"
     CDS             complement(10769..11890)
                     /locus_tag="IV328_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8938258.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(11892..12287)
                     /locus_tag="IV328_002146"
     CDS             complement(11892..12287)
                     /locus_tag="IV328_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8938259.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(12306..13103)
                     /locus_tag="IV328_002147"
     CDS             complement(12306..13103)
                     /locus_tag="IV328_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8938260.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(13105..13893)
                     /locus_tag="IV328_002148"
     CDS             complement(13105..13893)
                     /locus_tag="IV328_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8938261.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(13907..14383)
                     /locus_tag="IV328_002149"
     CDS             complement(13907..14383)
                     /locus_tag="IV328_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8938262.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(14455..17112)
                     /locus_tag="IV328_002150"
     CDS             complement(14455..17112)
                     /locus_tag="IV328_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8938263.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(17131..17535)
                     /locus_tag="IV328_002151"
     CDS             complement(17131..17535)
                     /locus_tag="IV328_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP8938264.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(17767..18024)
                     /locus_tag="IV328_002152"
     CDS             complement(17767..18024)
                     /locus_tag="IV328_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8938265.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(18149..18481)
                     /locus_tag="IV328_002153"
     CDS             complement(18149..18481)
                     /locus_tag="IV328_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938266.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         6026 a         3839 c         2809 g         6400 t
ORIGIN      
        1 ggtttcgctt ctggatgttt ggctttatat ccttcgtaat ccttactgtt gataattgct
       61 ttcatattag gaattggatc ttcacctggc atatattgca gattagaagc atgtaatttt
      121 ttctcagaaa caaaccactc attggaaaag acatgaatca aatcatcact tacttgtgta
      181 aaatagcgac gtttaatgac aaagatatct tcattgctat ctagtgtacc ttcatttctt
      241 tgtgcttaaa ttcaaagata attttctcaa taaagatttt tgattccagt aagattcctt
      301 tactcctttt acaacatagc gttgtccttt gttttgaaac atcttcttcc cctctttgcc
      361 attaaaaata gcccaattct ctataaagaa gattaagctt cattattata atataaagtt
      421 taaatttggt ttattactta ctttttctta ttctctagta agctaatatt gaaaaaaatg
      481 taatcagatc attattttta tatgttgtaa cttcctgata agaaaactaa ttaaaagaaa
      541 gaggtaacta cataaactaa ttaaaaaagc taacttcata gttggaggat aaatggaaga
      601 aacttcaatg attgtttctc cttgtgttcc ttcaatcgac actagtccta ttatcaatct
      661 ctcttttttg tccatttact tttgcgactg ttccccaata atcaaataca ggaagcgtta
      721 ttttttgtcc tttagattca tgtactgtat agactatttt cgaatccatc acttgataag
      781 gcatagtatg ccattccttg ccaattctta cttcatgatt ctgcaaatgt tctttgtatt
      841 gtaatgcttc cgtgggtgca tagtcataaa gtcctccggg aaggagtgtc gtgtaacgaa
      901 taagattttc ttgattcatt accaagtgat ctctctgagt gacaacttta tgttcatagt
      961 ttgcttttgt tgcagcagat acagccaaca gtaataaaac aattgccgtt atattaacta
     1021 cttttctttg cttagctgat gtgctcttca tgaaaacgaa cgataaaatc aacgagccaa
     1081 acagtacttg aaagcctaag attcgccaag gaaattgaat catttgaatg ggtgtatttt
     1141 gtaataaaga ccatggaaaa aatttggtgg ataagagcca agagactacc gcactaaggt
     1201 aaatcacttt ttctttgcga ttaaatcctt taaaaaagaa gagtggacag atcaaagaaa
     1261 taataccaat gattcctacc gtaaaggttg tcaggtcatt attcatcgca ccaatcaaac
     1321 tatccgagac atttaatgct tggtgttgta aggtcgtttt atctggctca ttaatcgatt
     1381 gaaccatgat ttgtcggaac atcggatacc aaaagtatgc tgttaaaaga atcgtcattc
     1441 ctactgcctt tgacaactcc acaatcaacc ggatgctttc tttttttctt gtttcactta
     1501 ctacaaagcc gcctaagact agtaagccaa cggctattac actcataaaa gtggaaagaa
     1561 catgtgtata aatgagtaag ctcatactga ctgctaatgg aattgcacat ggctttttct
     1621 cataaatcag tgcgtaagca taatacataa ctatcggtat aaccgtaagt gcaatcgctt
     1681 cagcaatagc agaacgatga taaatatcaa tcgtacggta cccataaaat gtatataaac
     1741 agctaaatat aaaagccgca tcgattcttt ttaaaaattt tagtccataa tgaaacatca
     1801 gcatgatagt acaaacattc aatagccaaa catataagat ataactaacg attaaattat
     1861 tacttatcca ataaaaaatg actgccggaa aaaaggtcaa gtagggataa aaataattca
     1921 ctccgctccc ataatgatta aacgtcgtaa aattgattgg accagaaaaa attgaagata
     1981 accctttgac tctagcaaaa tggaaggcaa tatcccccga tgtagaaata gtatgaacat
     2041 ttctaaaata attgattgaa taaagtccag caataattgc taggagaatg gacaatatta
     2101 tttttttctt tacttgcatt acttaattat atactccttt catggattgc tttatctagt
     2161 ataacttaaa ttttaaataa atcgttattt atttttactc tcacttaaaa aagcactaaa
     2221 attataagag agggaggtaa tggtctgaac accaagaatt gttccaacat cgaattgctt
     2281 aactaatgag ttcaaagctt aattatgtgg ctcttatatt taccgagcgt ggattgcgat
     2341 ctctttagcg aaacaaacat ctttcataaa aaaaacagcc atatccgctt tcttggatat
     2401 gactatttac tactacttta ctatctttca ttcttcttgt ctcacttatg ataaggcgct
     2461 gcgtatcatg agtgaatgat attaggaaaa cttcataaaa taaatctttt atacatttcc
     2521 acttctagat ggtcattcat gttagaagaa tcaaactatt tttttactgt cgtaataata
     2581 ttaaaacact agaacaaatc aattaaattc cataaaatat aaataataga cctagcatca
     2641 tctggtataa aatggatgct aggtccgtta tatttactca aaattgattc tacgaataga
     2701 tcttcttatc atttctattt tagtagtcat gcgttgttgt tcttctaaag gtggtaatgg
     2761 tataagtagt ttagataaat ttgtaccatt tacatttggt tgcccagttc cgtaagaaat
     2821 tttttctaat aacttccaat ataatggcga gtctaagaag cagtctacgt attctgataa
     2881 taatttctca actaacctaa ttcgaatcaa gtaagaagca aaaacagatt cctcacttat
     2941 ttccttaacc aagaaacttt ttcccatagt tcctcctgtc cttgctatca aaatgtcatt
     3001 ttcttccaat cttaaatcaa ctagtttaga attactaata tcacagtaag gcacactgga
     3061 ccaatttaca cgtccctctt gtatatctgt gattctcaaa aacttaacat tgccttgttt
     3121 tttcgccgaa tctgtatagc catattgaat cttagtagaa atacttccta aataaaccca
     3181 tagtcgaaca tcgctagatg atttttcttg tcgttcttta tgaacaacag tgatcatctt
     3241 gagttgtccg actatttttt agttgcagca atctttttgg gacgcttatt tgcatgaagc
     3301 aacgttgcta agtctagtaa taaaacattg gctaacacag gattggcttt ctcgcgatac
     3361 gctgcggcgc cactttcaaa caatactgct agacgatctt ctacaacaac gatttgttcc
     3421 aaatacggtg gaaatttgat tcgttttagg atccggctag gagagtcccg ctcggacact
     3481 aagagttccg aggtataagg accataagaa cgactggcta agagataatg gccataaaaa
     3541 gccacacctt gaatcttatc agatgttttt ctgctcattg tcacggttgg ttggattttg
     3601 tctttgcggc gaattgctgt tttcatttca gctatcgtcg gcaatagata gaaagtcaac
     3661 tgtccttcac cttttagtgt gaagttgcca gataccagtt gatcattgtt ttgagtcaaa
     3721 tacgatgctt gtggaatctc tgagagatca gttgtcagcc aataagcgat tggctgtccc
     3781 gtctcttcag atgtatctgc taagatatcg atcaaacgta aagcggctac tcgtccagta
     3841 gcagatcctg tgattgtcaa ccataaaagt tcccgttttg tatcatatac taagccccct
     3901 acatgtggcc gtttaggtaa aatcagcagt ttgatcggct gtcctgtttc tctatccagt
     3961 acatgaataa tagaatgatg ctgatggtca tagcagtagg cagaaataaa cacaaaatct
     4021 tgagaaaaag ccaacccttg cggcgtcatc cattgacaaa cctcattttc gccggtttcc
     4081 ggcaccacca ttcttgtttc tgttaatcca ggaatgttat attgttttgc ttcagccttt
     4141 tcccaagctg cttgaacaat aggataccgt tttaactttt tttcaatatc ctgatgtatc
     4201 cgttgctgct tccaatgctt aaactttagg acatagtaaa caagaaggcc aatgaccagc
     4261 aacactaaaa tcattattgc tacccgctgt gtcatttttt tgcctccttg ttctattcga
     4321 tcaatcgtcc atataccgct attaattacc gtaactacat gtttctttta ctagcgtttc
     4381 tttataggat ctttcataat ctcatttata gtaacattaa gaatccctta ttctactata
     4441 gtcacttcca catcaatatt gcctcttgtt gctttagagt atgggcaaaa tgcgtgggct
     4501 tcttctgcga ttttctctgc ttggtccttc tcaagtcctg gcaaagaaac ttctaaaacg
     4561 accccgattt ggtagccccc atcttctaat tgaaataaag atacccgtgc tttgacttca
     4621 gaatcagact caagattatt ttcttccagc atgtgttgaa ccgcgccgtt gaagcaagat
     4681 gcgtatccag ccgcaaaaag ttgttccgga tttgtccctt cactatggat tcctgggcgg
     4741 tcgatcttca tgtgcatact tccgtttggt gcctcaactt cgccatcacg gcctccacga
     4801 ttgatcattg ctgtttcgta catttttttc attgagatca tcctttcttt cgtcgttcta
     4861 tattaagtgt aacgagtatt ttttaatctg acaaaggtaa tgcttgcatt cggcgacatc
     4921 agttagacaa catcgatgat cagtgataga agcagtttat ctatacaaaa aaagaagggt
     4981 tctatgatat ttggtgtcct tactcgcctt gagtaacaac accaaatatc atagaaccac
     5041 ttaatcagct tcacactgct ttttctactt tcgtagttga aaaaaactta aacaaatcat
     5101 cggcaaatag ttaaagaaaa aacttaatcg ttgaaagaag cccaattgct taaagaaggc
     5161 aaactgacta atcgacggaa tccgtgctaa tccgtaaaga cctgcaatta gtaaactaat
     5221 aactgttaaa acaagataaa aatggctaag cgtaccttga ccactttgac gataaagcag
     5281 aacaagtaac aatgggaaaa gtagaaagcc tgcatagcct agcccagaac ctatattatg
     5341 gatccaagtc gatagattac aagagctttc attcgtattg atactgaata agccagtaaa
     5401 aatacaatca cctatcccat ataaaccaac tgttgcaaca acaagtattg caagtgtttt
     5461 tgatgttcca acaaataggt gatacaacgc aggcaacgaa agaacaaaaa aaagtccaga
     5521 tacaactgac cagaccaaga aagcacgtct gactggacta tctacgtcac caaacacact
     5581 aattacggta gtcatttgat tcagtttggg ataaaaaagg ccaaggatat acggtgtcga
     5641 taaatcactc atcacaccta ataataaaaa ataaaaacca tatttttgca aaaaaatcat
     5701 tttcttcccc tactttttag tgattattcg atacatccaa caatcaaatt ttttctcatt
     5761 ttaacacata tttttattct agttgtcttt tgaacaatca ataacataaa ggtgagtttc
     5821 ttctacattt taaaaaatcg aatggcactg aagagttcag ttgtcagcat caatctccta
     5881 ctcaaaaagg caaaaaaacg taagtcagat gttgttgaca tctcacttac gcatccattg
     5941 attgaaaatt tcttagtaaa atctactagc ctaaaaattt gaagacttct tctcgatcat
     6001 acagtttaaa tactttctcg cataaaaatt gagtaattag tgccgcaaaa acagggatga
     6061 ctaaccaaca aagaacaaca agaaagccat ttagccctgc atctaaagaa gccaacggac
     6121 caacaagtcc gaccacgccg aaaccagcag aagcaggcgt acctgaaata gaaaatagag
     6181 cgacaggaat agctgtaatc actgcactaa ctaaacaagg aataagaatc acaggttttc
     6241 taaatagatt aggcatcatc atcttcatag cgcctaaagc aatcgcaatt gtcacacctg
     6301 acttattaac cttccaagag ttcactacta aaacaaaagt ggttgcagct acgcccatcg
     6361 cagccgcacc agcagaaaca ccatctagtt ggattgccat tccgattgct actgttgaaa
     6421 taggagagat aatcaaaaag gcaaaggaac aaccaatcaa gatagacatt aacagcggtt
     6481 gtaaggtagt gaagttatta atcaatgatc caattgcaga agtaattgct gttacaaaag
     6541 ggaataaata aaaaccaagt aaacctgcac ctacacctac aacaatcggt gttaacacaa
     6601 tcgacactga cccaaaacta tttccaatca gtaaaatcag cccgacagca atcgatgctg
     6661 tcagcatcgt attaataata tcgcctgtac cagcaccaac gtaaacaccc gtttcaccca
     6721 aatcaggtgt gaatttgact acaccagaac caacaaaaga agctccggct acaaccatca
     6781 ttctcatcgg atcaaatcca aattgtaagg caatcaaacc accaatgatc aaaggtgttg
     6841 ctagttgaaa aataactgct aactgtgcga tcgttaccgc aaagggatac gcactaaaat
     6901 attttaaaat ggcacctaat actgcattag gaatcaatcc aataatgatt cctgttgctg
     6961 taccagcgag gactttattc agaaaaattt tcggtgatat tttttgtgaa gtttccattc
     7021 aatctctcct ttattttacg ttcaaactat cttcttttgc atggacaagc tcagtcaaga
     7081 aatcacaata aggcgtcgca acaccatatt tcttcccttt tctagaaatt gcgccattga
     7141 tataatcgat ttctgttaat cgatggttct taatcaagtc ttgatacatt gaaggataat
     7201 gtaacccaat tgtttccggg tcaaaacaag attcacaatg tgcaatgact tctgggacat
     7261 ctagttcaat cttctctact gctgctactt tggcaaactc attgacaatc gtcgccacca
     7321 ttttatgagc agtcgatgtt tttcctagct cagccatatt aacgtctaag attgtgcata
     7381 gtccgttcat tgttccgttc acacatgctt tgcgataaat agaatagtga atattgtcag
     7441 aaaaatgagc atttaatcct gattcagaca atttgtctgc taattttttt gcagctgctt
     7501 cttttccatc acctaaattt tgtaattcta ctgaaccact tccaaataat ttgacctgac
     7561 caggaccttc aagacctgcc gtccacatgg tatttccaat atagatattt tccattggta
     7621 caaatttctc aataatatct tcatgcccga taccatttaa tagacataag acctctgtat
     7681 cttttttgat taatgattgg atatcctgca gcattttttc cagctgcatc gctttggtaa
     7741 atagaataat cagatcaact tgatcttctt tttctacttc ggattgaaga acgattggta
     7801 gttttgcttc tacctcttta ccattaaaat ttgcttgtaa tccatgttct ttgatttgtt
     7861 ggacatgttc tgcccagcca tcgattaata atacttcgtt cccactttgg tgaagcatca
     7921 acccaaagcg acttcccatt gcgccagcgc ctgcaattgc tattttcatt gattcttcct
     7981 cctttttctt tctattatac tcatttgaga atcttttcac aacctcgttt tgttattctt
     8041 ctcttattta tcatgtttat atccaccatt caagttattt tatcagcaaa tcaataaaaa
     8101 ctggaataga atcccaattt catcgtaaga gaaactagac tagcaataag aaaatagaaa
     8161 cattcataga aaaaatctca ctcctctgat tataaaagag ggggcattaa cctcgtgtat
     8221 actaaaacct tccattcatt cgatggatga ttttcttttt gtgaaaaaat attcattgta
     8281 gtacgatgtt gcgcaacaca gacggatggt attgcttatt atttattaaa atcgaacaat
     8341 acacttattt aaaaacttct ttcccagatt ttcttccatt tttacagtta taactagtat
     8401 tatcatgaaa aatagccttt tattgtctat gggaataata atttaaaatt ggtacttttt
     8461 tattaattag ttcatcgccc aataatctct ccagtttctt agcatctcct gactcaaccg
     8521 gaattatcca agcccagtct aaataatttt ttaaaatctc atcatatttt gcaaatattt
     8581 cattgccata ttccgtcctt aaaattatat tttcagtgcc agacaagtga tttatattga
     8641 gttgataaaa aagatctcca tctaaaaaaa tatcattaga agaagatttc cccacagtaa
     8701 tgaccattcc atcagctcta tgtgttacat acaaataatt gtatgacctc atttcaccta
     8761 atgaatcaat tatttcttgc cctttagctc taatatattg atctctatta cctatctcac
     8821 tcttatttaa caaaagtttt ttcatgtcaa atttaatcgg ctgttttatt tttgtaaatt
     8881 cgcttctact actaatccaa tccgagatta atttttcaac atgtcccaga gtaaactctg
     8941 atttattcgc taaatacttt ttttgttgta ttaaagacat ctttccccga agctcaaaat
     9001 tgagcctaca tcgattctct atctcttcaa aaagcatctt atcttcatca ttcttatatt
     9061 catcattcat aaaaccaatc cagctcctcg attaagataa tattttaatc tcatacaaaa
     9121 actagctctc atcaaaacaa ccattgtttt gatttctcat gaaatttctt tatacttttc
     9181 tccgattata aattcctaac cccttgagaa aattacgcat atatttatct ccgcaaggct
     9241 taaaatttcg ttggccttct tttctcaaaa atgccccgat ttcgcctttt gatggataga
     9301 tttctgcctc agcaaaaacg tctaaaatat catcagtggt catagacaag gcgattttta
     9361 atttttttag caacacattg ttgatatatt tgatttcctt gcttttttgc ggcgtgtcta
     9421 ctatcggctt atctttttgc ggaccacgcg ccaagagaat caggccattt aggaatgcgt
     9481 caaaaacttg gttgttgatg gttttttcat acacattatt ttctgcatta tctttgttct
     9541 tatcctgtac tttggtcagt accgcctgag cttcttcttt agtcagtggt acaccgccta
     9601 gctcaaaagc tttaatcaaa tccgtatctt tcaaatcaat cgcataacgc aatcgcaata
     9661 acctatcatt gttattcatt tgttcctccg aagtatttag tctttacttt cttacgtact
     9721 ttttgtcttg ctaagatttt taggatcaat gataaaaacc gcatcactta tgataaggcg
     9781 ctgggtatca tgagtgaatg atattgctga atacaaatag tccgaccaca ttaagttgat
     9841 cggacttcat aagcatttta ttgactaata cttttttgct taagaattga ttctaacatc
     9901 ttttgctaaa gttaagcatc ctatagttcc tgcattatct gctaacttag gtgtgacaat
     9961 gtattcttcg atatttggcg tatcgacata gccgttaatt agttctaaaa aacgtctttg
    10021 tactttcttt ttcagatgtt cttgttgcat gacaccacca ccaaaaatta taatatctgg
    10081 tgaaagcatc agggtcgtat tacaggcaca ttgtgcaata taatccgcct cgatttccca
    10141 gaacggatct tctatagaaa gatcttgtcc cttggttccc gtacgcatct cgatagcggg
    10201 tccagatgcc aacccttcga gacaatcatg atgaaaagga caagtaccag agaaagtatc
    10261 tcctgaatgt ctcttaacta acgtatgccc catttctgga tggctaaacc cttcaacaaa
    10321 aatcccatct tgtatagctc ctccaccaat tccagttcca acagtgtaat aaacaacact
    10381 ggataaacct tttccatgac ctgaaacata ctccccgtaa caagcagcat tcacatcggt
    10441 tgtccaggca attggaattt cgaaatgctt attcaacgtt ccaacaaaat caaaattttt
    10501 ccaggcagtt tttggagtag aggtgatatg tccataggtc tttgattcac gatggatatc
    10561 aatcggtcca aatgacccta ctccaatgct ttctaattgt tctttatagt gattaaagaa
    10621 atcaattacc aaactcattg tttcattcgg tgtagttgta ggaaaactca ctctttctaa
    10681 aacctttaaa tcatcattgc caatggcaca aacaaattta gtcccacctg cttctattga
    10741 gccatacatt tcttactcca ccgtcctttt atctaggaat tcgttcttcc ctgtctaata
    10801 gtttttcgaa aaactcatgt ggttcctttt ggtaccaata ggcaacagaa gaaatatcgt
    10861 cttgacgctc ataaagcttg atgtcatcat ttcctagggt ttggaaagta acacgtaaat
    10921 tttctttgaa actaatgggg tcgggtaaat gccagcggta caacccatgc atcggtaaag
    10981 catcatcgcc aaaaccatga actggatttg gattaggttt tccagcacta aaataatccc
    11041 gggtgtgatc tcttttagat tgaaaaggat agcccataaa tagcgtatta aaattttgag
    11101 catatgccct ttctccaaag ttctttttat ggaaagccca agcgccgcca aaataatctt
    11161 ctgctcctgt agatgttatt gtagggaaat cttcatctcc atctatataa aacttaaatt
    11221 ctccttcccc ccaccagtat ctttccaatg cgcacaaagc aaagtaggtt cctacataat
    11281 acccagtgcc tttgattcca tcaataaggg tgtaatcttt tccttttgtc gtgatgggtt
    11341 cacggttcca tttagcatga aaatacatcg catcatccgg tacttcagtc aatgcataat
    11401 taaatgtata aaaaatgtgc ttaataaact caggatgttc attcgttaaa gtaatcttag
    11461 ctttttttcg aaatggcatc tcaaaatagc aattcatccc tgatgttgga ttcactacga
    11521 ttggtaagga attcacttcg catcttgtcc caaacccatt acaaaaaaaa tctcctaatg
    11581 gactttctac agaaggagtg gattcatcat cccaataaat acgcaaaatt aaatcacgta
    11641 agacgaaact cccagcgatt gttttgtctg tcactgtcat ccaaaaatga cgtaaaattc
    11701 cagggccttc catttctgct aaagtaattg tctctccagc atttatcgga acagaaggtg
    11761 acccttttct cccaattcct agatgactcg ctgtcattgc cgctttccct ttttcaccat
    11821 ttttattttc tggtgaaata gttctacttt cctctccctt gtggagccat atattattta
    11881 aacaattcat tttattcgtc ctctatcctt tcttcacttt tttgattatt catatagatg
    11941 atttgttgtt gtgcctccgc aataattgct ccaatatcta aattatcatt tcctaactgc
    12001 aaagcgattg acattgccaa gggaagattc attcctgcaa ttacgtgcac tctgtcagaa
    12061 atataggtaa aaaacttttg atttacactt cctccagcca tgtcagttaa aacaattatt
    12121 tcgtctgtag tatccactaa ctctttaatt tgttcatcta tatcatcaat ttcttcgaca
    12181 tatgcacata agctactaat ttgattatgc atacctgtta aaaactctag tgtctcttta
    12241 aaaccctcag ctagtattga atggctagca atgatcaatc ttctcataat tcaaatcctt
    12301 ttcctttacg ctaatattcc aaatgcagcc ataaccatcg caaaaacaat aacgagtaga
    12361 atcaaaatgg tcatactgat ttttttctta ctgagtagat aatatgctgc agcagtaata
    12421 attactggaa ttaatgttgg taaaattttg tccaacattg gttggatttt catactaact
    12481 tcaccaaaag tgaatttaag tggcgttgaa acactgataa ctgaagcaat taatgatcca
    12541 actactgtta gccccaatac tgaagcagct tcagttaaat aattaatctt ttcaccgtat
    12601 tcagtaacaa tcttcattcc ctgcttataa ccaaattcaa agcctctcat tcggaaaaag
    12661 aagattacca tgattaaaat gaaccataaa aagattccta caggattatt ttctaaagcc
    12721 atataagccg caattgatcc catgatggtt gggatcatcg tcatcaaaag agagtcccca
    12781 ataccagcaa ttggacccat cgtaccaact ttaaagtcat taatcgcatc ttttgcttct
    12841 aatccttgct tttcctccat tgcaacacaa gcacctaaaa taatggctgc cagccaaggc
    12901 tgtgtattgt aatactgaaa gtgattattt aaagcttcga tgtattcatc atcatctttg
    12961 tataattttc ttaaaatagg tgacaatgcg taagtcacag atgcaccttg ctgtgtttga
    13021 taattaaagg tacaaactga catcaaccaa cgatagttcg ctttcctaag atcctttttc
    13081 gttacttgat atttttcgct cattttattc atcctcccca ataaacccaa catcagaagc
    13141 tcccgcagaa gcagtgattt gtgcatcttt ttcagctcta tgatacacat taaatgctaa
    13201 agcgaatcct atgatcgccg ctccaataat aggtaccttc aaaaaagcgg ctaagaaaaa
    13261 tccaacaagt aaataactaa tgtatcgacc tgtaggcata aagcgtaaca acatcgcaat
    13321 accaacagca ggcaacattt ttccggctaa tgttagccca tccataaacc aattaggtaa
    13381 aacatccaaa acgtaattaa cagcagactg tcctaaagta acggcaataa atactggaat
    13441 tgctgcagat aaaccaaaga aaattggccc caaccacaaa acactattca ttttcttaaa
    13501 ttctcgttta ttcgcatatt cctgtgattt gcgaacaaca aaaccattta agattttgac
    13561 aattacatct agttgcaccc ctaacatacc tacaggtagt cctattgcta atccggcttc
    13621 tactccaagc tggctaccaa atgccgttgc aataattgcg cacgttccat agtccggtac
    13681 agatgagcca ccaaaaccgg caactcctaa tgacatcaat tgaactgtac cgccaactat
    13741 caaacctgtt tttatatcac ccatcaaaat tcctgtgaat aatccccaaa aaactgcgta
    13801 atttacaaag atcatcggaa tcccataata atcaacatgt ttaataaatg ctaatatagt
    13861 cagcataata atttgaaaaa tagtaaaatg cattatcctt actcctttat cctaattttg
    13921 attgaatttc ctgtgcctta tccgccggaa caaattgtgt aacaatttct acaccagaat
    13981 ccaacaattt tttatacgtg ctgatatttt catctgatgc ataggctcta gaagatagct
    14041 gtaatgcatt atctttaaca acagtaccgc caacaattag tcggtcaatt ctaataccgt
    14101 tatctactaa atgaaggatt gtttccggct ctttcacgat gacaaatact ttttcctgat
    14161 cgtatttccc ctttttaaaa ttcgcaatcg ctgtttcttc agtgataatt gatacagcca
    14221 ttccagcagg tctagctaaa cgcatacttg atttttggat gtcgttattt gcgacagaat
    14281 catcgatcac catcactctg tttgctttag acattggtgc ccattgcgtg acaataatcc
    14341 catgcaataa acggttatca actctcgaca taacaatact cataaatact tcctccttta
    14401 tctttcattt tcttacaagc aattaccatg ccaaaacaaa aagaacttga taaatcaagc
    14461 tctttttaca taatcataaa cgtatttaac ctcatgaatc gggatgtcga cactataata
    14521 atgttcaacg acactgaaag attgcttaaa atgtgtctga aatttttgaa attcctcact
    14581 cgtttcattg aaataaattt catcttccaa agtattttgt tttactaatc gttcaattaa
    14641 gcaactaatg tgaacatata atccgaaaca cgtattatta tcaaatgaaa ttcctagatc
    14701 attttgtaag atgtatatag catctgaaac ccgttctaat aatttagtcg gatttaatat
    14761 ggtcaattgc tccattaaat tcgttagact aaaattcttc agaatattat tgttcaaaac
    14821 tttcagttca ttatcattaa agtgtctatt caattgattg gagaatttac ttatattcat
    14881 tcctaaaatc atatcttcaa ttgaaaagaa ttctacatct tctagatgtg gatttagtgt
    14941 tccaacaata aaaagaatat tgttctcttt acgaagctcc gataaatagt tttcattcac
    15001 taaagagtaa tagtcacacg tttctatgac aagatcagta tcccttggaa agctatcttc
    15061 aagtatcttt tttaatttaa acgaagttcc taatccgctt gcacaagaac agacaatcat
    15121 atccttctta atatttgatg attcataaat ataatccaca ttttgatttt cttttacttg
    15181 ttcaagaatc tgtttaagcg gtaaatctaa taatatctga tttgcaacat caatagccat
    15241 tcttgttgaa acattgttta gtagtccgaa ttttttttga gcgtctagtt ccaaatttag
    15301 ataaattttc tccagtgaac ccatatcaac aagtaaaata atttcttcat atccggtaca
    15361 ctcttgtaag tatctattaa ttttgtctgc aatctcacta cttgaagtca ttagtggcat
    15421 atcaatggcg tcgtatatat atctctcaac gatcctatta gcagtacttg ctatacaggt
    15481 tgccgttaga tctccatgag ccaaaatcag tgctaaacgt ttttgctgaa attctttact
    15541 acacattaac ttaaataaaa taaccaaaac atttttttga atttttgtta aataaatatc
    15601 ccctatttga ataaaacgat tgactaattg aacttctcga ctacaccaac tattaatatc
    15661 attgatgaag gaatataatt tatcgtccga tgttaattgg atttgataaa tccaagaatt
    15721 caaaaagata gacgttattt gaagataata atagtcaaat tctatttgaa aatctctctc
    15781 tatttcctta acatactgtc taaatcgaat ctcttgatag gtgggatatg ttaaagctga
    15841 attattttgg atatcaacat tcttttgtct aaaatatcta tcatatttct caaaaagctg
    15901 ctgcttatca gtgtctttct tccacgtttg ataaagatac tctataagtt cgttgatatt
    15961 aaaaacttcg tctacttttt ccaaatcatc catttgaata tattttaatt cattctgtct
    16021 tttcataacc ttcatgccgt tgaaattcac ccattgtggt aaatgataca tctgaatata
    16081 aagttcttcc aaatcatcta agaaataaaa ggcattggca caggtcaatt gaatgatatt
    16141 tttcaaattg ccaacatttt cactaaagcg atagtttaat aaaaaatcta ataattgttg
    16201 agatatcagt attttctttg ctagtctttc ttcttctgat ttaaaaaata tcttgattaa
    16261 ctgttttctt tcataaagag aacgactgtc taatgttgga agtgttagct ttacaggtat
    16321 tcttctcaac aaagttgata atagtttatc ctcaggtggt tctgatgttg caaatataat
    16381 ccagcaactt gatgtgtacc agttttcatt atcacccatc cgatgaaatt tcctcgtatc
    16441 cataaactga aaaagtttct cctgacattc attgctcaat gcatgaattt catctaaaaa
    16501 taagactcca ttattagcta attctgctaa accctttctt ccatcttcgg ctcctgtata
    16561 tgcacctttt acatgcccaa aaaaattgga caagaaaagt tctggattat tggcatattc
    16621 actacaatta actgaaatga aagatgcctt ctcttctata taattattgg ccaacaaatc
    16681 cttatacaca taatttgcta aatagctttt tccagttcca gtctcgccat gaagtaaaat
    16741 gggtaagcca tttggcggat agtagattgc tgctttaatt tgagcaatta catttcgcag
    16801 acttccttga cttccgataa ccctttctat tccaacccta aaagagctat cataccattt
    16861 ttccgcttcc tctgaagagc taaaaatttg ttgatttgga actttctccg gtaaagaaaa
    16921 gaagattact ggacgagtat tgatttttcc aaattctcca tcttttacga attcattcaa
    16981 atattgagag acaatattac gactaacatt taatttttca gatattttct gtgctgtatt
    17041 gttcccccat ttatttttaa tctcagagtt ctctatgaca taattgacaa tgcgttcttt
    17101 taattcttcc atcacagcac ctcctcgaca ctattcgaat tttgccgaca gtatttttcc
    17161 ataaatatga cttgctaaaa aggctttgtt tagatcattc aattggaacg ttgcttgttt
    17221 tgcttgatcc gctaagacgt tgaaaagata ctctggcgaa atcgcatacc ccaaaatatc
    17281 aacgattgtt gcaatcagat cttcttttga ttcttcatct gaaagtaatt cacgatacaa
    17341 catcgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
    17401 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agccccaata aataattttt
    17461 atattctgac gcatccattt tactacggag attatctgcc gcactaaata acttctgatt
    17521 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
    17581 tattttagca tactcagttt tagaaaaagc aaaaaaataa agaaacttat tcttgataac
    17641 aagctacgtg acgtatatcc ctaacacaaa aatagccata tcctttggtt tggatacggc
    17701 tatttgatat tactcattca attatcaaat cacagtaggt gtgaatctca tcgggatcgg
    17761 caatatctac ttctgattag gatcaaaggt gattttctca ataaaggtgt gatacaaagc
    17821 tttaacttgc ttcttctcac tctggataag aaagcaatcc aaactagtca atatctgttg
    17881 gacatctttg acttgaatcg gttccccttg ttgttgaaag atccctaaga tttcattctc
    17941 tcgaatttgt aactgtcttc gtttttcagt caattcattt aaacgatctt ttaacatcgg
    18001 aaacaattcg ggactatctt ccaaagcagc ttcctgtttt tgctctaata gttagatttt
    18061 gcgtaccatt ttcttctttt attttccttc gattgctgat tttaatatag tctaaaggaa
    18121 atttatattt attgtattct ttataacttc aagagaactt cgtttgagta ttactgtatt
    18181 tttttaattc cataagaaaa ttagttatta cctgccacct aatcagcaaa tctgaacctg
    18241 agtcaatata attaatatta ttttccatct ccaacattgt aagtactgtt ggtgttaata
    18301 tagggacttt gtacaattgt atttttttga ttttatcatc tgcacaaagg agatttttta
    18361 aatctaatcc ttcttttatt tcaaaagttg aattttttaa atctataaga gtaaaaccaa
    18421 ttccttcata ctgaatttct gaaaatcgaa tttttctccc agttgataga tcaaggggca
    18481 gaaaaccttt tattacattg tgagtgtcac catcatttac aatgtcatta ggaattttga
    18541 ttttagaatt ctgtggagca cctattggaa gataataatt cattataatt ttgtagtctt
    18601 catgaggaaa taaagtactt ttaggattgc taagatattc tcgtaccgaa tctaagaatg
    18661 gcttatccat tgaatcttca tccccattta aactaaaaaa cattcctaca attgcttttg
    18721 caactctaag tgaattcatt ttctttattg aatgtgttaa atcagactta ccattcttta
    18781 aacttgtatt gttataaagg aaatttttat tgtcttctgc aaatctaata tattcctcaa
    18841 catactcact accaaaaaaa ctattacatt tactacataa cgtattggaa tggcaacagt
    18901 ttttttgaca aattttataa ggtgcagaac ttctttccgt atgctattcc gattgtcctt
    18961 gacaatgagc ctcctcggat gtgacgatct tcggcaggag ctaacagtta aagttagact
    19021 gcctcgctag cgttagcaca tccgagctca ttatcaagga ttcgctgcgc caat
//
LOCUS       DACDKJ010000058        18849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDKJ010000058 DACDKJ010000000
VERSION     DACDKJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.058"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(159..578)
                     /locus_tag="IV328_002154"
     CDS             complement(159..578)
                     /locus_tag="IV328_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938267.1"
                     /translation="MKKSHIVTLATIFLLCVAAFFFFTNSPKNNKHITASTATTESTI
                     KTETVYPSLNELKQLQGSDIEVLGKTQITTVLFQNKNMAILTTENNKEIQLPLIVTDK
                     KNNLFEIPSLTYSKKNLKVGEVFGLAKKDNHYLAYKL"
     gene            complement(624..866)
                     /locus_tag="IV328_002155"
     CDS             complement(624..866)
                     /locus_tag="IV328_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938268.1"
                     /translation="MNITNIEELKEWIETNLVTKQEAIKITEQSPKGFQQSVRTNVIK
                     PFYESKEGKGPSVVRLYLKSDIETYAKQLKEKKQKP"
     gene            1017..1916
                     /locus_tag="IV328_002156"
     CDS             1017..1916
                     /locus_tag="IV328_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8938269.1"
                     /translation="MKLLKVLAVGVTVGGIALAINQEKVSAEEWTPRTVEQIKADIKG
                     NEYTIVWGDTLSGISQATNITVQKLADINKIANVDLIYAGNKLVFEGNVATVQNSAGE
                     TVAQTVIQDTDKVNPTQPVGQPAVSQDTTSNATNSADQSVAPTTPAGDSGANTPEQPV
                     QPSTPSDNGNQGGTVTPTPNPDQNQGGNNNQDNQGNQGGDTGNNGNQDNQGNNNGNNN
                     NGNNNGGNEGGDVTPPQPVEKWTVWYSASDEANAAHNIEKGTKVFDTEAEATAWIDQY
                     ADGLLAQGITSNSYGVSSFLVQE"
     gene            2169..5279
                     /locus_tag="IV328_002157"
     CDS             2169..5279
                     /locus_tag="IV328_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP8938270.1"
                     /translation="MILGALAMFLGFGLSSQSVNADNITMKPGEKGDIYYDASNVYTM
                     HGTKHGQPWSDATAAMAVSPNSGATDRIVFCIQPGVPFINTNNPDYEAVAVTDIPKDA
                     QIASVIWNTFYAFGTNFTNEDRIVTQAVIWELLPQYGIKVDSISGIPDFQAKKQKLYD
                     GIKEYKRLPEFNNKTIDLTFGETKKLTSSVDLNSFETQVSNSAKVNWNIAADGKSVDV
                     TPTDPNVTGGSISFKRSYMEGTPIALEKTGSQTVYLPSVKDPNNYSVNFRIQTVGDIT
                     IKKVDKETGEVVPNTQFKVEFSGKGAPSAQTVTTDKNGLATIKGINHGVHVKATEIAV
                     PAPYVLGSAIGDSDVVEGDVVAGQTVTLTQRNQKAKGQILIEKSGVETGKDMWNANYT
                     LAGNVFEIHEGTETGAVVRTITTDDKGHAETGKDLPLGTYVITEKTASNGFANTFKPV
                     TVKIEYANQTTAVIIKGTEGTNQEVTGSTLLTKEDAETGKETQGRATFDGAQYGLFHK
                     DGTPVKWSENFKPTTSKGNKLEGDEVKFELSDKEQQASVEHLALGDYYWQELVAPEGY
                     QIDNTKREFSITYKDQDTKVIETQSTSQENVIKFSLDGFKYVDSKSGDTKTGYNGIDF
                     TLTPIDPTKGEQRKVTTETDENGYDGYWAFNEVPYGDYKMSEVKAPEGYKTIKDLIIN
                     SKFDAEKREYTFTITEDGQKEPIKTLTVPEAKINEGSNVIHLSKLFLTNKVKEMPTIR
                     TKATVDGEKTFTPSKETPMQDVATITNVEKGVEYTNKIKLWRIVNDDYDNAKVVWEGS
                     HDFVAEGDKAEDVIKQMVDTSKDDNTVSYVFTEELFIKDGAKVAEHNDLTNKEQTIRP
                     VVPELPTIETLFVTKDGGKTFDPTKDQTLIDEVKATVPKEDIGQTFYYVTQFHKIDKN
                     GKVTVVGTDESEHKAGEMTYEFKAVYNYKANTLKDGEKLVATHIAYTDKEHKNEYAKH
                     FDLKNEKQTLTAKTPVTPATPQKSIPQTSGSLVNIGSLIVLGIATVAGAALYVTTKKK
                     VNKNEK"
     gene            5328..6365
                     /locus_tag="IV328_002158"
     CDS             5328..6365
                     /locus_tag="IV328_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8938271.1"
                     /translation="MFLGKGEINIVKKIIKALPLLWLLSSLILAICGFYGYTVLLRPE
                     NTIKQAQQITHPIQSEKEIKEIQQTTIGKATYNENQIQPVTPTDYAEAQLSYEALVNQ
                     WGIGSLYIPSSGIYSKILAGMSNDNLMVGLGTYYPNQLLGKGNYVLMAHNLVQGGGVL
                     RNLPQSSVGSTIYATDFSKIYEYEITTNKIVNQSESKLLDIPQEGDSPLMTIFRCEGG
                     LHTANRALIQARYVRSYSAENGSHDIKQALGLETTRNKTVNKQRLNDQQATSTNRTEA
                     AKESITPDKDTKKAKQTNKIEWRFTEKKAIYSNFQVFSILVFQLANAYPILIELVFLV
                     GLSSCILLNRI"
     gene            6452..7978
                     /locus_tag="IV328_002159"
     CDS             6452..7978
                     /locus_tag="IV328_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8938272.1"
                     /translation="MKKKHISKVASAVLLSQMLLTTPLQVLADEQLSTPTSQSSTMNN
                     QSEESTSSSDEQTGTSTTQTTTDSTESSTDQSTTDDTTTSSTDTTSTTESSEKPTEDS
                     TTDSTSTTESSSESKPDETKPSEIKPSESKPEKPNTGSSTNESQTKPTTQPSNNQQAP
                     SVAPNNQATATNEGQQAKPAESESKPYHQTPTAPIQELVPSSKETAEGNIHFEKDESV
                     ESFVRKIGESARKVGQENDLYASIMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGK
                     GVSFATQEDLGDGTLYTTQATFRQYENYEDSLNDYAQLLKEGLTGNSQFYSGVWKTNA
                     KTYQEATKFLTGRYATDTKYDQKLNGLIETYHLTDYDKEIAGPQLSKEGYIVPVKNYT
                     ISSPFGTRGGEFHRGLDLAAAQGEPIYASKTGTVIKAEFHPSWGNYVAIEHEDGTTAL
                     YAHQQEYQVKVGDKVKQGQIIGYVGSTGNSTGSHLHFELCLDHSLSQSQLVDPETVLF
                     "
     gene            8156..8563
                     /locus_tag="IV328_002160"
     CDS             8156..8563
                     /locus_tag="IV328_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938273.1"
                     /translation="MTNRNEGITIYLTIGKEVKLNFDSENNLSSIRLSINKVAELFGN
                     KVVNEESIFYVSTVALVTKSNYKTFISVVGGKQVISGDAVYEEVMIDQERTSNKRGNK
                     NVYEQGYFEFFVSEEDKIPKEKLILPYNVKKRR"
     gene            8587..9741
                     /locus_tag="IV328_002161"
     CDS             8587..9741
                     /locus_tag="IV328_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3801 domain-containing protein"
                     /protein_id="HAP8938274.1"
                     /translation="MNITVKELGEPIELEDYLEEYKFQVIFLLKNMTFIDRKDNRVRQ
                     LFNMELIRAETKWYSFYQKNHRPPDYNFLQEEITSFGVNRLPMFDRRNEGRVNDELSV
                     DNFVHYHIQQLKLEKILSPIFFEKNDLRFMERYAKNKAKAYFNERDQYIKGYESFRIS
                     RNKIMQRAGYEQLKYEFVSDNMVQGYFKEEVAKMAANDERVAEFVFDEGKSLMRNLTQ
                     KMSRGGVSIVTGSTKAVTNQIKKQRTTGKQRYNQLKKHGALEHIDVSKTDLKPLANEL
                     KKYKVDFAVKRNKETEKIHVFFKAQDRDTLNLALENVLQKFANEKEQNREQQQEKPKK
                     DIEKSSTDGKDKDDSLSKKMDKFEEKAQEINQQREQLKENTKNKTKGQER"
     gene            9816..11612
                     /locus_tag="IV328_002162"
     CDS             9816..11612
                     /locus_tag="IV328_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8938275.1"
                     /translation="MVYFSIPQNTDSSWINVVNEAFDKFLEPKTFTISPFTFSKIAMK
                     GGAIIAVIVLLIDLYIISGKKNLRMGEEHGSAKWGTSKDIAPYVDKKFKNNLLFTNTE
                     RITIETKLKKNKEYERNNNVVVVGGAGSGKTRFYVLPNLLQMHSSYVINDSKGGILSD
                     TAAIMQQNGYRIKVFNLNTMKNSDGYNPFHYIRRTEGDCEIDILKVVNNLMKNTSDPN
                     KKGGDSFWDDAQRSFLAAIFHLLILKGKPEEQNLGIVADLVRRARVKEDDEEYISSID
                     LVFQELEQEQPSCFAAKQYQNFKMAAGKTAKSILISLGVRLSPFDVPDIRKLTSTDTL
                     ELDTIGDRKTVLYIILPDTHTTMNFLASMIYQQLFDTLVYKADNVYNGSLPIPVRCLL
                     DEFANTGQIPDFENVIATTRSRGISVDVILQNLTQISKKLYKDSWETIIGNCDSFLYL
                     GGNEQSTHKYISTQLGKETIDVVTYNESRGTTGSFTKNSQKQGRNLLDPNEVREIKGG
                     KCIYMLRGTKPFLSDRFKLERHPLFKKLKETPSGYMYQSPLDQEEFIANNDPIDFELS
                     KNVDMKEFLKHTEIETYDFEDEEIETINLDEL"
     gene            11629..11832
                     /locus_tag="IV328_002163"
     CDS             11629..11832
                     /locus_tag="IV328_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019640568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938276.1"
                     /translation="MGTFNAIIDIVSKFAVIGGGFYAVWGAIVLGGALKDHNGPQMQS
                     GIWQIVGGAVIVAAAALFKSIAA"
     gene            11856..12707
                     /locus_tag="IV328_002164"
     CDS             11856..12707
                     /locus_tag="IV328_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938277.1"
                     /translation="MMDGFLTWCVESILEVISFSSKTSHLTSNLENFSPQLYKYSTTI
                     LDSVSKPIAFSILALFFALELQRISNRVETNGGGQTGIEMIFKSLLRLGLCKIAMDQV
                     SLIMRAIMDITGYMTDKINHLGMGNKEVSDMLDAKKILAPILKEGLMFKVGVSTVLII
                     ALLITIGSIVIVQVVVNMRFIELYAFLAMSPIPIATFPNDEWSSIGKNFLKSFTATAL
                     QGTLIFMVMQFYPYLLSSAFSDITNSGSASETFLMTLIGIIGNSVLLIFAVMATGRWS
                     KSITNAM"
     gene            12972..13343
                     /locus_tag="IV328_002165"
     CDS             12972..13343
                     /locus_tag="IV328_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP8938278.1"
                     /translation="MAIRVKVPKEIKDYQEKLMGGMSVRQLVFFVLAVLISVGVTAYC
                     VLVLHMSVDAAGYIVIPLLIPIVAFGWIRKEGMPLEKYGKIILNYYSKTGIRIYQTKE
                     RSNQNDFRKKRFQKVKETTKR"
     gene            13291..15741
                     /locus_tag="IV328_002166"
     CDS             13291..15741
                     /locus_tag="IV328_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8938279.1"
                     /translation="MILEKNDSKKSKKQPKDKQAKKVLKILAKQKKQRGKNQRRDAPS
                     LSSQRSLTYKAMYENGVCELKEGLYSRTFKFSDINYAIADQSEQELTFSSYCELLNSC
                     DSDTSLQISIINRSKDKKQFEKDLSYELVDDGLDVYRKEMNHMISNKIKYGNSSLQKE
                     KYITFNQRAKTLEQAIPALNRLQGDLEDQFSEMGCEVEELSGIERLRVVNDILRPNKK
                     LHFEYEDLLFSGLTTHSAIAPDSFDFKYRKDRFKMGDYYGQVLYLRNYPAELSDKLIT
                     NITDIPTNLQITLHIEPLDQQKGLDLVKTKLSFMEQQKIDEQKKAARSNYDPNLIPME
                     LRRSMDEAQELLDQMQGDNEKLFSFTFLVMAVDKDERKLDDTIEQILTAGRKNDCEFA
                     KLNYLQEEGLNSTLLWGNNFVPIHRTLTTASTAVFIPFTAQELMQPNGKYYGLNSVTK
                     NLVTLDRRKLKAPNGFILGTPGSGKSFATKKEMTAVLLSDPDAEVIVIDPEREYTPLA
                     EALNGEVIQISAGSKNHVNPMEINNDYADDDDPVILKTDFVLSLCEMLVGGNIGLSPT
                     QKTLIDRSCRFAYKKYFENGSGMPTLKDFSNILKEQPEDEARQLAVELELYTEGSLSV
                     FSHQTNVDTSKRFIVFDVKDLGKQLRSMGMLIILDQIWNRITSNRNAGRRTWVYIDEI
                     QLLFSNEYAENYFFELWSRARKWGAIPTGITQNVSTLLSSLNAERMLSNSDFVLMLNQ
                     SQRDRERLGELLHISSRQKKYITNAKEGAGLMYAGDSMIPLYDEFPKDTNLYKVMSTK
                     IEDVYKYQNEQREQGTTV"
     gene            15738..17585
                     /locus_tag="IV328_002167"
     CDS             15738..17585
                     /locus_tag="IV328_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP8938280.1"
                     /translation="MNTDFRERIIRKQRTAKKFVVNRDNNQFKKKIVSTDHTKRAKKA
                     NHSGKIKSSRGTSYLIKKDSAKNVVKDVNSKAQKNGVEQGNATAELETSKTIGRGIKN
                     STKATKTVAKTTTKVAKKSKELIQTNASKKEVLTVVKKGSKKGLVNTSKAAKDTVINE
                     IQEFRGDSESQGINEAIQLKDFIDTGITGAKKTKKAANTAKKSSKALTKFGKSSYKYT
                     QRIAEIGIRRVQQVLSKEVAKALIAKLAIPLTLAVLILSIVTAVISTVNIFNFGAGDC
                     DTSANYDNITTKDIESNAKSIYSIIRKEIPESTPQGISGALGAMQFESQLNPSAVNPS
                     SGATGIAQWLGSRLTSLKEFAHKKGKKETDLGVQVEFLLSELNSSYYQSSKKILAMTD
                     VHEACKEWVMKFEGLSQDSSQWYLDQRNSYADHWYATLGTSDPIAEGTISNGADSELN
                     TLTCDLESDGGDIMGIAKSWLGWFHYDQIHPAPDLGTDLKNPNKNGRTDCSGFVWLVL
                     NKAGYKVPANMQWFTGSMASDARGSHQYLKQISETEAKAGDIVIVNQGAGAGSNGHTG
                     ILTEKWRGKDTKIIQEGGNGDSVNIEAFGTSFMSLLDGGDVVLARPIKK"
     gene            17606..18226
                     /locus_tag="IV328_002168"
     CDS             17606..18226
                     /locus_tag="IV328_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938281.1"
                     /translation="MLNRKSVTLIVLVLIEAIFCFSIGLSINQELTVNSKITALEKKN
                     DALKRETKDQKSRISKLEDENNKMLETQVVNKDGDVYKEFTNIATKYFKSRFNYDPKT
                     YKESKENVRNLISEELYKKFSENQLTYGDSNNVSSRLDNLEIYSGSLINQKTITGLLI
                     VDYESKLGETDWFKNSEIYTVQYDIHAKKLTKIQSLGSFLRGDMIE"
     gene            18219..18593
                     /locus_tag="IV328_002169"
     CDS             18219..18593
                     /locus_tag="IV328_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8938282.1"
                     /translation="MNKKFFSICVLIFTVSIIFLADHSYIKYIEAQNDPVYLDESNQE
                     KIVFYRNDCRDCEKIMPYLIRHNFLYHDLTFVNMNNKENKRYIKEYGLTSVPTIISLK
                     QSYVGTDISKIKTLVMSKGDDF"
     gene            18593..18826
                     /locus_tag="IV328_002170"
     CDS             18593..18826
                     /locus_tag="IV328_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938283.1"
                     /translation="MARKSLTERIQQKKEEATKLEKRIQEDQYKIEKLTKQIRDLEAE
                     QVLAILDNHKLSVKDLNKILSKQPDNNNFINHN"
BASE COUNT         7057 a         2786 c         3590 g         5416 t
ORIGIN      
        1 acaaaagaat gtcagcttat tacagtattt caattaaaca aaagttaacg attatgatct
       61 caaaatatca ttttatgatt ttgatacaat aatcacaata ttatatagta aaatttttta
      121 aatgaggtgc aaaaatagag agctaaatgt ctccctattt ataatttgta tgctagatag
      181 tgattatctt ttttagctaa accgaagact tcaccaactt ttagattttt ctttgagtaa
      241 gtgaggcttg gtatttcaaa caagttgttt ttcttatcag tcacaattaa gggaagctgt
      301 atttctttgt tgttctctgt agttagaata gccatgttct tattttgaaa taaaacagta
      361 gtaatttgag tcttccctag aacttctata tctgaacctt gcaattgttt tagttcattc
      421 aaagaaggat agacagtttc tgtttttatt gtactttctg tggttgctgt actcgctgtg
      481 atatgtttat tgtttttggg cgagttggta aaaaagaaaa atgcagcaac gcaaagtaag
      541 aaaattgttg ctaatgtaac gatgtgtgat tttttcatag catatctcca ttaatatcaa
      601 tttataaaaa gcagaaactg taattatggt ttctgctttt tctcttttaa ctgtttagca
      661 taggtttcaa tatcgctttt gagatagaga cgaactactg aaggaccttt tccttccttg
      721 ctctcataaa atggtttgat cacatttgtg cgaacagact gctgaaatcc tttaggagat
      781 tgttcagtaa tcttgattgc ttcttgttta gtaactaaat tagtttcaat ccattctttt
      841 agttcttcta tattagtgat gttcatatta atcccacctt tctgtggttt tgattgtcta
      901 cagaaatatt ataaatgaaa aaaagtaaaa aatctaatat aaaatggttg caatactatt
      961 ccattactgg tatagtatta atataataaa aattaaaaag aaagaaggaa taaaacatga
     1021 aattattgaa agtgttagct gtcggtgtaa cagtaggagg aattgcgtta gcaatcaatc
     1081 aggaaaaagt gagcgcagaa gaatggacac cacgtacagt agaacaaatt aaagctgata
     1141 tcaaaggcaa tgagtacacg atcgtttggg gcgacacttt aagtggtatt agccaagcaa
     1201 caaacattac tgttcaaaaa ttggctgata tcaacaagat tgcaaatgtt gacttgatct
     1261 atgcgggaaa caaattagta tttgaaggca atgttgcaac tgttcaaaat agcgcaggtg
     1321 aaacagtagc acaaacagtt attcaagata cagacaaagt taatccaact caaccagtag
     1381 gacaacctgc agtttctcaa gatacaacaa gtaatgcaac taactcagct gaccaatctg
     1441 ttgcacctac tacaccagct ggagatagcg gagcaaacac tccagaacaa ccagttcaac
     1501 cttcaacacc ttctgacaat gggaatcaag gtggaacggt aactccaact ccaaatcctg
     1561 atcaaaatca aggtggaaat aacaaccaag ataatcaagg aaatcaaggc ggcgacactg
     1621 gaaataatgg aaaccaagac aatcaaggaa acaataatgg aaataacaac aacggaaaca
     1681 acaatggcgg taatgaaggt ggcgatgtaa caccaccgca accagttgaa aaatggacag
     1741 tttggtattc tgcttcagat gaagctaacg ctgctcacaa tattgaaaaa ggaacaaaag
     1801 tatttgatac agaagctgaa gcaacagcat ggattgatca atatgcagat ggcttgttag
     1861 cacaaggtat tacttcaaat tcttatggcg tatcttcatt tttagttcaa gaataataaa
     1921 atctgtttac caagacactt aagaaaattc ttaggtgtct tttttttacc cttttttcaa
     1981 attgctattg aagggtttat tttcttaagg ggggtgattc caatctaaaa tttaatgtca
     2041 gtcggtggag cgagtctttg gggggcgctc tttttttatt gtacttatat atattgattg
     2101 aagagaggga gattatagtg agaataaaaa acaagagaaa taagtggaaa gttcccaagt
     2161 atctattgat gattttaggg gcattggcaa tgtttttagg atttggactt agttcgcaaa
     2221 gcgtaaatgc cgataatatt acgatgaagc cgggagaaaa aggcgatatc tattatgatg
     2281 caagtaatgt ttatactatg catggtacga agcatgggca accatggagc gatgcaacag
     2341 ctgcaatggc agtttctcct aacagtgggg ctacggatag aattgtattt tgtattcaac
     2401 ccggtgtgcc ttttattaat acaaataatc cagattatga agcagttgct gtaactgaca
     2461 ttcctaaaga cgcacaaatt gcttctgtca tttggaatac gttttatgct tttgggacta
     2521 actttaccaa tgaagatcga attgtcacac aagctgtcat ttgggaatta ttaccacaat
     2581 atggaatcaa agttgactct atcagtggta ttcctgattt tcaggctaaa aagcaaaaac
     2641 tttatgatgg aatcaaagaa tataaacgtt tgccagaatt taacaacaag acgattgatc
     2701 taacatttgg cgaaactaaa aaattaactt cttctgtaga tttaaattct tttgaaactc
     2761 aagtttctaa tagtgcaaaa gttaactgga atattgcagc tgatggtaaa tcagtcgatg
     2821 taactcctac tgatcctaat gtgacaggtg gaagcatttc ttttaaacgt tcatacatgg
     2881 aaggaacacc aatcgcatta gaaaaaacag gctcacaaac agtctactta ccaagtgtaa
     2941 aagaccctaa taactacagc gtgaatttcc gtattcaaac agttggagat attacaatta
     3001 aaaaagtaga taaagaaact ggcgaagttg ttcctaatac gcaatttaaa gtagagttta
     3061 gtggtaaagg agcgccttca gcacaaactg ttactactga taaaaatggt ttagcaacga
     3121 tcaaaggaat taatcatggt gtgcatgtca aagcaacaga aattgctgta ccagctcctt
     3181 atgttttagg atcagctatt ggcgattctg acgtagtaga aggcgatgtt gtcgcaggac
     3241 aaacagttac tttaacacaa cgtaaccaaa aagctaaagg acaaatcctg attgaaaaat
     3301 ctggtgtaga aacaggaaaa gatatgtgga atgctaacta tacattagca ggaaatgtat
     3361 ttgaaattca tgaaggaact gagacagggg cagttgttcg cactattaca acggacgaca
     3421 aaggtcatgc agaaacagga aaagatttgc cattaggtac ttatgtaatt actgaaaaaa
     3481 cagcttctaa tgggtttgct aacacattca aaccagtaac agtgaaaatc gaatatgcaa
     3541 accaaacaac agcagtaatt ataaaaggca ctgaaggaac gaaccaagaa gtaactggtt
     3601 caacattgtt aacgaaagaa gacgctgaaa caggcaaaga aacacaagga cgtgctactt
     3661 ttgatggggc acaatacgga ttattccaca aagacggtac tcctgtgaaa tggtctgaaa
     3721 acttcaaacc aacaacttct aaaggaaaca aattagaagg cgacgaagtg aaatttgaat
     3781 tatctgacaa agaacaacaa gcaagtgttg aacacttagc attaggtgac tactactggc
     3841 aagaacttgt tgctccagaa ggctaccaaa ttgataatac gaaacgtgaa ttttctatta
     3901 cttacaaaga tcaagataca aaagtaatcg aaactcaaag cacttcacaa gaaaatgtaa
     3961 tcaaatttag tcttgacggc tttaaatatg ttgattcaaa atcaggcgat actaagactg
     4021 gttacaatgg aattgacttc acgttaactc ctattgatcc aacaaaaggc gaacaacgta
     4081 aagtaacaac tgaaacagat gaaaacggct atgatggata ctgggcattc aatgaagtac
     4141 cttatggcga ctacaaaatg tcagaagtaa aagctccaga aggctacaaa acaattaaag
     4201 atttaatcat taacagcaaa tttgatgctg aaaaacgtga atacacattt accattactg
     4261 aagacggaca aaaagaacca attaaaacat tgactgtacc agaagctaaa atcaatgaag
     4321 gttcaaatgt gattcattta agcaaattat tcttaactaa taaagtgaaa gaaatgccaa
     4381 ctatccgcac aaaagctact gttgacggag aaaaaacatt tactccttca aaagaaacac
     4441 caatgcaaga cgttgctaca attactaacg ttgaaaaagg tgtcgaatat acaaacaaaa
     4501 tcaaactttg gagaatcgtt aacgacgact atgacaatgc taaagttgtt tgggaaggtt
     4561 ctcatgattt tgtagctgaa ggcgataaag cagaagacgt tattaagcaa atggtagata
     4621 cttcaaaaga tgacaacact gtctcttacg tatttacgga agaattattc attaaagatg
     4681 gagcaaaagt tgcagaacac aacgacttaa cgaataaaga acaaacgatt cgtccagtcg
     4741 tgcctgaatt gccaactatt gaaacattat tcgtaacaaa agatggtggc aaaacatttg
     4801 atccaacgaa agatcaaaca ttaattgatg aagtaaaagc aactgtacct aaagaagata
     4861 tcggtcaaac attctactat gtaacacaat tccataaaat tgataaaaat ggaaaagtta
     4921 ctgttgtagg tacggacgaa tcagaacaca aagcaggcga aatgacttat gagttcaaag
     4981 cggtttataa ctacaaagcg aatactttga aagacggcga aaaattagtt gcgactcata
     5041 ttgcttatac agacaaagaa cacaaaaacg aatatgccaa acactttgat ttgaaaaatg
     5101 aaaaacaaac actaacggca aaaacaccag ttacaccagc aacaccacaa aaatcaattc
     5161 cacaaacttc tggatcattg gttaatattg gaagcttaat cgttctagga attgctactg
     5221 ttgcaggtgc agcattgtat gttacaacta agaaaaaagt aaataaaaac gaaaaataag
     5281 aatgaagggg aagcactttg aaaaaggtgc ttttcctatt ttagttattg tttttaggga
     5341 aaggagagat caatatagta aagaaaatca ttaaagcctt accgttattg tggctactaa
     5401 gtagcctgat tttagcaatc tgtggatttt atggttatac cgttcttctt cgtcccgaaa
     5461 atacaatcaa acaagcacag caaatcactc accctataca gtcagagaaa gagatcaagg
     5521 agatacaaca aacgacaata gggaaagcaa catacaatga aaatcaaatc caacctgtta
     5581 cacctacaga ctatgcggaa gcacagctga gttatgaggc tcttgttaac cagtggggaa
     5641 tcggttctct ctatattccg tcttctggta tatactccaa gattcttgca ggaatgagca
     5701 atgacaattt aatggttggc ttgggaacct attatccaaa ccagttactt ggaaaaggaa
     5761 actatgtttt gatggcacat aatttagttc aaggtggggg agtccttcgt aatttaccac
     5821 aatctagcgt aggatcaaca atttatgcga ctgattttag caagatttat gaatatgaga
     5881 tcactacaaa taaaattgtt aaccaatcag agagtaagtt attggatatc ccacaagaag
     5941 gtgattcgcc tttaatgact atttttagat gtgagggagg attacacaca gctaatcgtg
     6001 cattaattca agctaggtat gttcgatctt actcagctga aaatggttca catgatatta
     6061 aacaagcttt aggattagaa acaacgagaa ataagacagt caataaacaa cgtctaaatg
     6121 accaacaggc gacttcaact aatagaactg aagcagcaaa ggaaagtatt actcccgata
     6181 aggatactaa aaaggcaaag caaacaaata aaatagaatg gcgcttcaca gaaaagaaag
     6241 ctatttattc taacttccaa gtgttttcta tcctagtatt tcaattagct aatgcttatc
     6301 ctattctgat tgagttagtt tttcttgttg gtctctcaag ttgtattcta ttgaacagaa
     6361 tataatgagt gacaaaactt atatatattt ttataaaaaa ttcataaaat aaagttatct
     6421 tagatgtaac acaggaaagg aaagagaaaa agtgaaaaaa aagcatattt caaaagtagc
     6481 ttccgctgtt cttctttctc aaatgctatt gacaacaccg ttacaggtat tagcagatga
     6541 acagctttca acgccaacca gtcaatccag cacgatgaat aatcagtctg aagaatctac
     6601 ttcaagtagt gatgaacaaa caggaacgtc tactacacaa acaacgactg attccacaga
     6661 aagtagtaca gatcaaagta caaccgatga tacaacaact agctcgacag atacgacgtc
     6721 tacaacagaa tcgtctgaaa agcctacaga agacagtacg acagattcaa caagtacaac
     6781 ggaatccagt agtgagtcta aaccagatga aactaaacct agcgaaataa agccgagtga
     6841 atcgaaacca gaaaagccga acactggcag ttctacgaat gaatcacaga ctaagccaac
     6901 aactcaacct tcaaacaatc agcaagcacc gtctgttgct ccaaacaatc aagcaacggc
     6961 aacaaatgaa gggcagcaag ctaaaccagc agaaagcgag tcaaaaccgt atcaccaaac
     7021 gccaactgcg cctattcaag aattagttcc ttcaagcaaa gaaacggctg aaggaaacat
     7081 tcattttgaa aaagatgagt ccgtagaaag ctttgtccga aaaatcggcg agtctgctcg
     7141 caaagtagga caggagaacg acttatacgc ttctatcatg attgcacaag ccattttgga
     7201 atcagctagc ggtcaaagtc agttagcgca agcgccaaat tataacttgt tcgggatcaa
     7261 aggaacacat aacggaaaag gtgtttcttt tgccacacaa gaggatttag gtgacggcac
     7321 tctttacacc acacaagcca cttttagaca gtatgaaaac tacgaagata gcttaaatga
     7381 ttatgcacag ctgcttaaag aaggtttgac tggtaatagt caattttata gtggtgtatg
     7441 gaaaacaaac gcaaaaactt atcaagaagc aaccaaattc ttaactggaa gatatgcaac
     7501 tgatacgaaa tacgatcaaa aattaaatgg tttgatcgag acctatcatt taacggacta
     7561 cgacaaggaa atcgcaggac cacaattaag taaagaagga tatattgtac cagtgaaaaa
     7621 ctatacgatt tctagtccgt ttggtactcg tgggggagaa tttcatagag gattagattt
     7681 ggcagcagca caaggcgagc caatctatgc tagcaaaaca ggaacggtca ttaaagcaga
     7741 gtttcaccct tcatggggga attatgtagc tattgagcat gaagacggta caacggcttt
     7801 atatgcccac caacaagaat atcaagtcaa agttggcgac aaagtaaaac aaggtcagat
     7861 tattggttac gttggctcga caggaaatag cacaggtagc catttacatt ttgaattatg
     7921 tttagatcat agtttaagtc aatcacaatt ggttgatcca gaaacagtct tattttaaaa
     7981 ataaggctgt ttttttattc aatttttaag gaggtattgt ctatgttgct atttataagt
     8041 ggtttatttt taggcggatt tattggtgtg gcgttgatgt cattagtagc aatcgatcgt
     8101 tatgaagata tgattgatgg aagaagataa ttaagaaaga gggagtaaaa attttatgac
     8161 gaatagaaat gaaggaatca ctatctactt gactattggt aaagaagtta agttaaattt
     8221 tgattcagag aataacttat caagtattcg tctatccatt aataaagttg cagaactttt
     8281 cggaaataaa gtagtcaatg aagaaagtat attttatgtt tcaacagtag ccttagtgac
     8341 aaaaagtaac tataagactt ttatttcagt agtaggtgga aagcaagtta ttagtggaga
     8401 cgcagtttat gaggaagtaa tgattgatca agaaagaact agcaataaaa gaggaaacaa
     8461 aaatgtatat gagcaaggat actttgaatt tttcgtgtca gaagaagaca aaattcctaa
     8521 agagaaacta attttgcctt acaacgtaaa aaaacgtagg taaaaagctt atgtgaggat
     8581 atatctatga atataacagt aaaagaatta ggcgaaccta tagaactcga agattattta
     8641 gaagaatata aattccaagt catattttta ttaaaaaata tgacttttat tgatcgtaaa
     8701 gataatcggg taagacaact ttttaatatg gaactgattc gagcggaaac taagtggtat
     8761 tctttttacc aaaaaaatca ccgtccacct gattataact ttctacaaga agagattacg
     8821 tcatttggtg tcaatcgttt gccgatgttt gatagaagaa atgaaggacg tgtgaatgac
     8881 gaattaagtg tagacaactt tgttcactat catatacaac aattaaagct tgaaaaaata
     8941 ctgtccccaa ttttttttga aaaaaatgat ttacgattca tggagagata cgcaaagaac
     9001 aaagcgaaag cttatttcaa tgagcgtgat caatatataa aaggctatga aagttttaga
     9061 atatctcgaa ataaaatcat gcaacgagca ggatatgaac aacttaaata tgaatttgtt
     9121 tctgataata tggtccaagg ttattttaaa gaggaggttg ctaaaatggc agcaaatgac
     9181 gaacgagtgg cggaatttgt ttttgatgaa ggtaaaagct taatgcgaaa tttaacacag
     9241 aaaatgagca ggggaggggt atcaattgtt actggctcaa caaaagcagt aactaatcaa
     9301 attaaaaagc agagaactac aggaaaacag cgctacaatc aactaaaaaa gcatggagcg
     9361 ttagaacata ttgatgtctc aaagactgat ttaaagccac ttgcaaatga actaaagaaa
     9421 tataaggtag attttgccgt aaaaagaaac aaagagactg aaaaaataca tgttttcttt
     9481 aaagcgcaag atagagatac tctcaattta gcattagaaa atgtgctaca aaaatttgct
     9541 aatgaaaaag aacagaatag agagcaacag caggaaaaac ctaaaaaaga tattgaaaaa
     9601 tcttctactg atggtaagga taaagatgat tcattaagta aaaaaatgga taaattcgag
     9661 gaaaaagcgc aagaaataaa tcagcaaaga gaacaactaa aagagaacac gaaaaataaa
     9721 actaaggggc aagaacgatg aatatcacca aaaaagatct tatttttggt gtcgcacttg
     9781 ctgctttttt cttttattta ggtactttat tttttatggt ttatttctcg attcctcaaa
     9841 atacagattc ttcatggata aatgtagtaa atgaagcatt tgataagttc ttagaaccta
     9901 agacatttac catttctcca tttacttttt caaaaattgc tatgaaaggt ggggcgataa
     9961 ttgcggttat tgttttactg attgatttat atattataag cggcaagaaa aatttaagaa
    10021 tgggcgaaga acatgggagt gccaaatggg gtacttctaa agacattgct ccttatgtag
    10081 ataaaaaatt taaaaataat cttttattta caaatacaga aagaataacg atcgaaacta
    10141 agttaaaaaa gaataaggaa tatgaacgta ataataacgt tgtagttgta ggtggagctg
    10201 gttcaggtaa aactagattt tatgtattgc ccaatttatt acaaatgcac tcaagctatg
    10261 ttattaatga ctccaaaggt ggaatcttga gtgatacagc tgccattatg caacaaaatg
    10321 ggtatcggat aaaagtattt aatctgaata ccatgaaaaa ttctgatggt tataatccct
    10381 tccattatat tagaagaaca gaaggggatt gtgaaattga tatattgaaa gtagttaaca
    10441 atctaatgaa gaacacttca gaccctaaca aaaaaggtgg agattcattt tgggacgatg
    10501 cacaaagatc ttttttggca gctattttcc atttgttgat tttgaaagga aaacctgaag
    10561 aacaaaattt agggatagta gcagacttag taagacgcgc aagagtaaaa gaagatgatg
    10621 aagaatatat ttcttcgatt gatttagtat ttcaagaatt agagcaagaa cagcctagtt
    10681 gttttgcagc aaaacagtat cagaatttta agatggcagc aggaaagacg gctaagagta
    10741 ttttaattag tttaggtgtt cgtttgtctc cgtttgacgt tccagatatc agaaaattaa
    10801 cgtcaacgga caccttagaa ttagatacaa taggagatcg taaaactgta ctttatatta
    10861 ttttgccaga tactcatact actatgaact ttctagcaag tatgatttac caacaacttt
    10921 ttgatacgtt agtttataaa gcagataatg tttataatgg atcgttacca attcctgtaa
    10981 ggtgtttgtt agacgagttt gctaatactg ggcaaattcc agattttgaa aatgttattg
    11041 caacaacacg tagtagagga atttcagttg atgttatctt acaaaattta acacagatta
    11101 gtaaaaagct ttataaagac agttgggaaa caatcatagg aaactgtgat tcttttttgt
    11161 atttaggagg aaacgagcaa agtactcata agtatatttc aactcaatta ggaaaagaaa
    11221 caattgatgt agtaacttat aacgaatcaa gggggactac gggaagtttt actaaaaact
    11281 ctcaaaagca gggaagaaac ctacttgatc caaatgaagt gcgtgagatt aaaggcggaa
    11341 aatgtattta tatgttacgt ggcacaaagc cttttttatc tgatcgtttt aagttggaac
    11401 gccacccttt atttaaaaag ttaaaagaga caccttcagg ttatatgtat caatcaccgt
    11461 tggatcaaga agaatttatt gcaaataatg atccaattga ttttgaatta tcaaaaaatg
    11521 tcgatatgaa ggagtttttg aaacatacgg aaatcgaaac ttatgatttt gaagacgaag
    11581 aaattgaaac aataaattta gatgaattat aaaaggagag aataattaat gggaacattt
    11641 aacgctatta tagatattgt atcaaagttt gcggttattg gaggaggatt ttatgcagta
    11701 tggggtgcaa ttgttttagg tggtgctcta aaagatcata atggaccaca aatgcaatca
    11761 ggtatttggc aaattgttgg tggagctgtg attgtagcag cagcagcgtt atttaagtca
    11821 attgcagcat agtggatgta cagaaatgag gataaatgat ggatggattt ttaacatggt
    11881 gtgtcgaatc tattttagaa gtaatttcct ttagcagtaa gacaagccac ttaactagca
    11941 atttagaaaa tttcagtcct cagttatata agtattcaac aactattctt gattctgttt
    12001 caaagccaat agcttttagc atattggctt tgttttttgc tttggaatta caaagaatat
    12061 ctaatcgtgt ggaaactaat ggaggaggtc aaacaggtat tgaaatgatc tttaaatcat
    12121 tgttacgatt aggactatgt aaaatagcta tggatcaagt atctttaatt atgcgggcga
    12181 ttatggatat aacaggatat atgactgaca agattaatca tttaggtatg ggaaacaaag
    12241 aagtaagtga tatgttagac gcaaaaaaaa tattagcccc tattttaaaa gaaggattaa
    12301 tgtttaaagt aggtgtatca actgttttaa ttatcgcatt actaattact attggttcaa
    12361 tagtaattgt acaggtagtt gtaaatatgc gttttattga actatacgca ttcttagcta
    12421 tgtctcctat tccaatcgca acatttccta atgatgaatg gtcaagtata ggtaaaaatt
    12481 ttctaaaatc atttacagcg acagcgttac aaggaacttt aatatttatg gttatgcagt
    12541 tttatccata tctcttaagc tcggctttta gtgatataac aaatagtggt tcagcaagtg
    12601 aaacattctt aatgacactg attgggataa ttggtaatag cgtgttacta attttcgcag
    12661 taatggcgac tggaagatgg tcaaaatcta ttacaaatgc aatgtaggaa ggacttttca
    12721 atgaaaaaat ctcgttgtaa cagcactcta atttttggag tgctatttct tttgttacaa
    12781 atactatcca tattattaac acagtataat atttccaatc cattaacact aagtatactt
    12841 tatgtatttt tcattgtttc tcttgttttt acactcataa tgatcgtaat tattacaaaa
    12901 gagatgttgc gagattttaa gaaattaata tctgatattt tttcacagaa gaaagaaaag
    12961 taggtgtttg agtggcaatt agagtaaaag taccaaaaga aattaaggat tatcaagaaa
    13021 aactaatggg aggaatgagc gttaggcagt tagtattttt tgtattggca gtattaatct
    13081 cagtaggagt aacagcatat tgtgtattgg tattgcatat gtcggttgac gcagccggtt
    13141 atatagttat cccactattg atacctattg tagcatttgg ctggataaga aaagaaggta
    13201 tgcctttaga aaaatatggc aagattattc taaattatta ctcgaaaact ggaataagaa
    13261 tatatcagac taaagaaagg agcaatcaaa atgattttag aaaaaaacga ttccaaaaag
    13321 tcaaagaaac aaccaaaaga taagcaagca aaaaaagtat taaaaatctt agcaaagcag
    13381 aaaaaacagc gtggaaaaaa tcaacgtaga gacgctccat ctttatctag ccaacgatca
    13441 ttaacatata aagcaatgta tgagaatgga gtgtgtgagc ttaaagaagg gctttattct
    13501 cgtacattca aattttcaga tatcaattat gcaattgcag atcaatctga gcaagaatta
    13561 acttttagct cctattgtga gttactaaat agttgtgatt ctgatacttc tttgcagatt
    13621 tcgattatta atagaagtaa agacaagaaa caatttgaaa aagacttatc ttacgaatta
    13681 gttgatgatg gattagatgt atatcgaaaa gaaatgaatc atatgatatc taataagatt
    13741 aaatatggga atagtagttt acaaaaggaa aaatatatta cttttaatca aagagcaaaa
    13801 actttagaac aagctatacc agctttaaat agacttcaag gcgatttaga agatcaattt
    13861 agcgaaatgg ggtgtgaagt agaagaactg tcaggaattg agcgcctaag ggttgtcaat
    13921 gatattctac gtcccaataa aaaacttcat tttgaatatg aggatttact tttcagtggc
    13981 ttaactacac attcagcaat tgcgccagat agttttgatt ttaaatatcg taaggatcgt
    14041 ttcaagatgg gggattatta cggtcaagtt ttgtatcttc gtaattatcc tgctgaactt
    14101 tcggataaat taattactaa tatcacagat atccctacta atttacagat tactttgcat
    14161 attgagcctc tagatcaaca aaagggatta gatttggtaa aaacaaaatt atcatttatg
    14221 gagcaacaaa agattgatga acagaaaaaa gcggctagaa gtaattatga tcctaacttg
    14281 atacctatgg aattaagaag aagtatggac gaagcacaag aattgttgga tcaaatgcaa
    14341 ggagacaatg aaaagctctt ttcttttact tttttagtga tggcggttga taaagatgaa
    14401 agaaagcttg atgacacaat tgagcaaatt ttaactgcag gtcggaaaaa tgattgcgag
    14461 tttgcaaagc taaattatct tcaagaagaa ggtctaaact ctacgctttt atgggggaac
    14521 aattttgtcc ctatacatcg aacattaacg actgctagta cggctgtatt tattcctttt
    14581 actgcacagg aacttatgca gccaaatggg aaatactacg gtttaaactc agtaacaaaa
    14641 aacttagtga cgttagatag aagaaagcta aaagctccta atggttttat tttaggtact
    14701 ccgggtagtg gaaaatcttt tgctactaaa aaagaaatga cagctgtttt actgagtgat
    14761 cccgatgctg aagtaatagt aattgatcct gaaagagaat atacaccttt agcagaagct
    14821 ttaaatggag aggttattca gatatccgca ggatcaaaaa atcatgtgaa tccgatggaa
    14881 attaataatg actatgcaga tgatgatgat ccagtcattt taaagactga ctttgtgtta
    14941 tctctttgcg aaatgttggt agggggaaat attggtctta gtccgactca aaaaacttta
    15001 attgatcgtt catgccgatt tgcctacaag aaatattttg aaaatgggtc tggaatgcct
    15061 acattaaaag atttttctaa tattcttaag gagcagcctg aagatgaagc aagacaatta
    15121 gcagtagagc ttgaacttta tactgaagga tcattatcag tgttttctca ccaaaccaat
    15181 gttgatacga gcaaacgttt tatcgtattt gatgtaaaag atttaggaaa acaattacga
    15241 tcgatgggca tgttgattat tctagatcaa atttggaatc gtatcacaag taatcgaaat
    15301 gcaggacgtc ggacatgggt atatattgat gaaattcagc ttcttttttc aaatgaatac
    15361 gcagagaatt acttttttga attatggtca agagcaagaa aatggggggc aattcctaca
    15421 ggtatcaccc agaatgtgtc tacgcttctt tcttcattaa atgcagaacg tatgctatca
    15481 aatagtgatt ttgttctaat gttaaatcaa tcccaacgtg atcgagaaag actaggcgaa
    15541 ttattacaca tttctagccg tcagaaaaaa tatattacga atgccaaaga aggagcagga
    15601 ttgatgtatg cgggcgattc tatgattcca ctttatgatg agtttccaaa agatactaac
    15661 ttatataaag tgatgtcaac taagattgag gacgtgtata agtatcaaaa tgagcaaaga
    15721 gaacaaggta ctacagtatg aacacagatt ttcgagaacg gattatccga aaacaaagga
    15781 cagcaaaaaa gtttgtagta aatcgagata ataatcagtt taagaaaaaa attgtatcta
    15841 ctgaccatac aaaacgagct aagaaagcta atcatagtgg aaaaataaaa agtagtcggg
    15901 gcacttccta tttaataaaa aaagattcag caaaaaatgt agtcaaagat gttaactcta
    15961 aagcacaaaa aaatggtgtt gaacagggga atgcaactgc tgaattagaa acaagtaaaa
    16021 caataggacg tgggataaaa aatagcacta aggcaacaaa aacagtggct aaaactacaa
    16081 ctaaagttgc taaaaaaagt aaagaattga ttcaaacaaa tgcttcaaaa aaagaagttt
    16141 taactgttgt taaaaaagga agtaaaaaag gtctcgtaaa cacaagcaaa gctgcaaaag
    16201 atacagtaat aaatgagatc caagagttta gaggtgattc agaatctcaa gggattaatg
    16261 aagcgattca attaaaagat ttcattgata caggtatcac tggtgctaaa aaaacaaaaa
    16321 aagcagcaaa tactgctaaa aaaagtagta aagcactaac aaaatttggt aaatcaagtt
    16381 acaaatatac acaaagaatt gctgaaatag gtatacgtag agttcaacaa gttttatcaa
    16441 aagaagtagc aaaagcactt attgctaaat tagcgattcc actaacttta gcagtgctta
    16501 tattatcaat tgttacggct gtaatttcaa ctgttaacat ttttaatttc ggagcaggcg
    16561 actgtgatac tagtgctaac tatgacaata taactaccaa agatatagag tcaaatgcta
    16621 aaagtattta ctcaattatt cgtaaagaaa tacctgagtc tacccctcaa gggatttctg
    16681 gagcattagg agctatgcaa tttgaatctc aattgaatcc aagtgcagtt aatccaagct
    16741 caggtgcgac aggaattgcc cagtggttag gttctcgttt aacctcttta aaagaatttg
    16801 cgcacaaaaa aggaaagaag gaaacagatt taggtgttca agtagaattt ctgttatctg
    16861 aattaaatag ttcatattat caatctagca aaaaaatatt agcaatgaca gatgttcatg
    16921 aagcatgcaa ggagtgggtc atgaagtttg aaggattatc tcaagactct agccaatggt
    16981 atttggatca aagaaactct tatgctgatc attggtacgc aactttaggg acgagtgatc
    17041 caattgctga aggtaccatt agtaatggtg cggatagcga attaaatacg cttacttgtg
    17101 atttagaatc tgatggtggc gatattatgg gaattgctaa gagttggcta ggttggtttc
    17161 actatgatca gatacaccct gctcctgatt taggaacaga tttaaaaaat cctaataaaa
    17221 atggaagaac tgattgctct ggttttgttt ggctagtttt aaataaagct gggtataaag
    17281 tacctgcaaa tatgcaatgg tttactggat caatggcaag tgacgctaga ggatcacacc
    17341 aatatttgaa gcaaatttca gagacagaag caaaagcagg cgatattgtt attgttaatc
    17401 aaggtgctgg agcaggtagt aatgggcaca cagggatttt gactgagaaa tggagaggta
    17461 aagataccaa aattattcaa gaaggcggaa atggcgatag tgtaaatatt gaagcttttg
    17521 gtacgtcctt tatgagctta ttagatggtg gcgatgttgt attagcacgt cctataaaaa
    17581 aataattaga ggggaggaag tactattgct taatagaaaa tcagttactt taattgtatt
    17641 ggtattaatt gaagcgatct tttgtttctc cattgggtta agtattaatc aagaattaac
    17701 tgtaaattct aaaataacag ctttagaaaa gaaaaatgat gccttaaaaa gagaaacgaa
    17761 agaccaaaag agccgaatta gcaaacttga agatgaaaat aataaaatgc ttgaaacaca
    17821 agtagtaaat aaagatggcg atgtctacaa agaatttacg aacattgcta ctaaatactt
    17881 taaatcaaga tttaattatg atcctaaaac atacaaagaa agcaaggaaa atgtaaggaa
    17941 tttgatttct gaagagttgt ataagaaatt ttcggagaat cagctaactt atggcgattc
    18001 taataatgta agttctcgat tagataattt agagatatat agtggctccc taatcaacca
    18061 aaaaactatt actggtcttt tgattgttga ctatgaaagt aaattaggag aaactgactg
    18121 gtttaaaaat tcagaaattt atactgttca gtatgatatt catgcgaaga aattgactaa
    18181 aatacaaagc ttggggtcat ttttaagggg agatatgatt gaataaaaaa tttttttcta
    18241 tttgtgtctt aatttttact gtgtctatca tatttttagc agatcattct tatatcaaat
    18301 atatagaagc acaaaatgat ccagtctatt tagatgaatc aaatcaagag aaaatagttt
    18361 tctacagaaa cgattgtaga gactgtgaaa agatcatgcc ttacctgata cgtcacaatt
    18421 ttttatacca tgacttaact tttgttaaca tgaataacaa agaaaataaa cgttatatta
    18481 aggagtatgg tttgacttcc gttcctacaa tcatttccct taagcagagt tatgtaggta
    18541 cagatataag taaaataaaa acattagtta tgtcaaaagg agatgatttc tgatggcaag
    18601 aaagagctta acagaacgta ttcagcagaa gaaagaagaa gcaacaaagc ttgaaaagag
    18661 gattcaagaa gatcaatata agatagaaaa actaacaaag caaattagag atttagaagc
    18721 agaacaggtt ttagcgattc ttgataacca taagcttagt gtgaaagatt tgaataaaat
    18781 actaagtaaa cagcctgata acaataattt tatcaaccat aattaattaa atatatatct
    18841 ttttaattg
//
LOCUS       DACDKJ010000059        18848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDKJ010000059 DACDKJ010000000
VERSION     DACDKJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.059"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            159..1346
                     /locus_tag="IV328_002171"
     CDS             159..1346
                     /locus_tag="IV328_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8938284.1"
                     /translation="MQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKK
                     QVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIES
                     VTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWEN
                     TDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFE
                     SHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWS
                     SQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFD
                     FQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAV
                     QNK"
     gene            complement(1438..1803)
                     /locus_tag="IV328_002172"
     CDS             complement(1438..1803)
                     /locus_tag="IV328_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8938285.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1800..2165)
                     /locus_tag="IV328_002173"
     CDS             complement(1800..2165)
                     /locus_tag="IV328_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8938286.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2357..2605)
                     /locus_tag="IV328_002174"
     CDS             complement(2357..2605)
                     /locus_tag="IV328_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938287.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2604..2732
                     /locus_tag="IV328_002175"
     CDS             2604..2732
                     /locus_tag="IV328_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8938288.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2789..4483
                     /locus_tag="IV328_002176"
     CDS             2789..4483
                     /locus_tag="IV328_002176"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8938289.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4583..4756
                     /locus_tag="IV328_002177"
     CDS             4583..4756
                     /locus_tag="IV328_002177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938290.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5134..6267
                     /locus_tag="IV328_002178"
     CDS             5134..6267
                     /locus_tag="IV328_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8938291.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6298..6618
                     /locus_tag="IV328_002179"
     CDS             6298..6618
                     /locus_tag="IV328_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938292.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6647..7111)
                     /locus_tag="IV328_002180"
     CDS             complement(6647..7111)
                     /locus_tag="IV328_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8938293.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7243..8496)
                     /locus_tag="IV328_002181"
     CDS             complement(7243..8496)
                     /locus_tag="IV328_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8938294.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8525..8842)
                     /locus_tag="IV328_002182"
     CDS             complement(8525..8842)
                     /locus_tag="IV328_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8938295.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8868..9188)
                     /locus_tag="IV328_002183"
     CDS             complement(8868..9188)
                     /locus_tag="IV328_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8938296.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9652..11067)
                     /locus_tag="IV328_002184"
     CDS             complement(9652..11067)
                     /locus_tag="IV328_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8938297.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11084..12400)
                     /locus_tag="IV328_002185"
     CDS             complement(11084..12400)
                     /locus_tag="IV328_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8938298.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12550..13248)
                     /locus_tag="IV328_002186"
     CDS             complement(12550..13248)
                     /locus_tag="IV328_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8938299.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13537..14526)
                     /locus_tag="IV328_002187"
     CDS             complement(13537..14526)
                     /locus_tag="IV328_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8938300.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14606..15439)
                     /gene="msrA"
                     /locus_tag="IV328_002188"
     CDS             complement(14606..15439)
                     /gene="msrA"
                     /locus_tag="IV328_002188"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8938301.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15614..17248)
                     /locus_tag="IV328_002189"
     CDS             complement(15614..17248)
                     /locus_tag="IV328_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938302.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17235..18635)
                     /locus_tag="IV328_002190"
     CDS             complement(17235..18635)
                     /locus_tag="IV328_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8938303.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
BASE COUNT         5967 a         3713 c         3145 g         6023 t
ORIGIN      
        1 tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac tttactcaat gatatcaata
       61 acataaaaaa agaatttgaa gaacagatcg cttatacaca tcgggaaaag gaccgttaca
      121 ctttatattt ttcagaacat atacctcgat gcaagatcat gcagcaattg tctcaaaatt
      181 cgttgttttt aaaaacttgt ctgctttatt tggaagaaga tgaacctgat tatcttcaat
      241 taactgaatg tgaattcatc tctgtcagca aagcttattc attaaaaaaa caagtattag
      301 cttactttaa tgactgcggg atcgaaatcg accgctactc ccctcgattc actgaaatgg
      361 aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc
      421 tacctgaatc gttttttgaa cgtgccgatc gtttcattga atccgtaacg gaaaatagtg
      481 gcagatttta tgataaagaa aataaagaaa tcttatcgat tggttttgct atcagtttcc
      541 tacgccagca agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac
      601 gtcctattta caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata
      661 aaaaagaaga attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt
      721 cttatcagtt gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc
      781 ccgaaataaa agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa
      841 accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat
      901 tattcatgcc agaaaaattt tatcttttaa atgaggaaca gacaaattta tataaagaag
      961 tccagacaat tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta
     1021 attgcatgcg ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc
     1081 tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg
     1141 aagctgtaac tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata
     1201 taaagaagta tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac
     1261 cagatgcggt ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta
     1321 tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca cagcctctta
     1381 tttgttgaag atgcccattt ctgtaacaat ctcttgatac acgtcaggct cacaacctca
     1441 cttattttgg acatctaggg gtccgatctc tttatttaaa aaataagaaa tgatggttcg
     1501 gataactacc aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc
     1561 gatgatccca gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt
     1621 tttaatagca ttgttctcgc ttactcggtc gctttgcact tttgtatgga caaataaata
     1681 tttcaaaaaa tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat
     1741 atcaagtccc agtacaagtt tttcgaaata tggaagaaga aactgcatca actctgtcgt
     1801 catcctacct ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa
     1861 gaaatgactg ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc
     1921 ttgataactg actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa
     1981 cttgctagca gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg
     2041 acatgagacc aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg
     2101 atagaacaaa tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga
     2161 aacatatacg ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt
     2221 taccttgttc gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt
     2281 ttcactctcc catttttctc cctccatcca taaatcgtgg aagtatccaa aacaacgtag
     2341 caagttaaaa taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt
     2401 tttttgtacc caaatttcta aaaatagaac catacacctc ctcaaatctt ttcttcttac
     2461 ctaccctatt tttatttaca ggaaaatcag acacttcttc ttttttgtca aaatttcctt
     2521 tttcttctcg tcctatcatt tggcattcta ataaaaacga actatccttg gatatagaaa
     2581 gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat caaagtcggc atcggtttag
     2641 gtttgcttgc ccttggcgct gcatatctag gaaaaaagac aggactattc gaagatgaca
     2701 gtcatttata tgatgaattc gaatcaatct agcatcctat ttagttgaat cactacggac
     2761 atacttggaa gacattggag gaatcacaat gagaaaaagt aaagcaataa tgattggtgc
     2821 cggtattgcc aatatggcag cggctgttta tttgattcaa gaaggcaaat ggaaagggga
     2881 tcagatcacc ttttattcat tggatgatca cggttcaaat gatggtgcac cgaccgacac
     2941 tgtagtggat gaatattgga ataaaaacca tccattagaa aaccaaaaag gctatattgc
     3001 tcgaggcggt cgcatgctta attaccgtac ctatgtagat ttgatggatc tgttaagtcg
     3061 aataccttct gttactgagc cagggatgac tgctgaagaa gatacgagag attttgatgc
     3121 aaaacaccga acctttgaca aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg
     3181 cggaaaactg ggattcaata ataaagaccg tattcttctg actaaactga ttgctatgcc
     3241 tgattcagaa gaagaaaaac tagacaatat cactatcgaa gaatatttca aagatgatcc
     3301 acactttttt gaaacaaact tctggtttat gtgggaaaca acatttgctt tcagaacacg
     3361 tagttctgct caagaattgc gtcgctatat gcatcaaatg atttatgaat tcactcaaat
     3421 cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc
     3481 tttgatcgag tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga
     3541 ctgggaattc aaagaaacac cgatgcaaga tgaaatcact gtgactggtt tgaaaattac
     3601 caatacactg actcaagaag aagaatacgt tgtggtcgat gaagatacag cagttatttt
     3661 cactaatggg tccattacag attcagctac tttaggagat tacgaaacac cagctgtaga
     3721 aaatatggat tatggtgcag cttctagtct ctggaaaaaa gccagtgaac gtttctacaa
     3781 cttgggaaat cctgacaaat tctttgctga tcgagatgca agcgaatggg tcagctttac
     3841 cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc
     3901 aggaaatgcc ttgaattcat ttatttcaac cacaccgatc acgccgttag gccaaaaaga
     3961 tgtcaatatg tctattgtcg tgcatcatca accgcatttt acgtctcaga aaccaaatga
     4021 aacagttatt tggggttatt tcctttatcc aagacgacgt ggcgaatttg tggataaaca
     4081 gtatattaaa atgaatggaa aagaaatgct agaagaactg attggacaac tttcaaaagt
     4141 agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa
     4201 taatatccct gtctatatgc cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga
     4261 tcgaccaaaa gtcattccta aacattcgac caatctggcc tttacaggag aatttgtcga
     4321 gcagccttat cagatgatct ttactgaaca aagtgctgta cgttctggag aaatcgctgc
     4381 tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga
     4441 tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa
     4501 agctgccctg catcaagtaa taagaacaac caggctaaaa cagcttctta tgtttttgat
     4561 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga
     4621 aaaagctgcc ctgcatcaag taataagagc agccaagcta aaacagcttc ttatgttttt
     4681 gacttggtta gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
     4741 tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt
     4801 tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac ctctttttat
     4861 ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct tgccaatcca
     4921 acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa atcggaaaca
     4981 cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa aaaaagcgtt
     5041 tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac taataatgca
     5101 gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa
     5161 cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac agatgcaatc
     5221 atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca tcatggtgaa
     5281 gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg
     5341 ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt taatattagt
     5401 tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga atcaaatcca
     5461 gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca ttggggtggt
     5521 caagcggaat atctaagagt tccttatgca gactctactt catttgttgt accagataat
     5581 gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc ttattggagc
     5641 gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc tggtccagtc
     5701 ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat cgcagtagat
     5761 ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac ttatttctta
     5821 gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc
     5881 attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc gaaaaattta
     5941 gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa
     6001 tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta cccgctgcaa
     6061 gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat ccatctaatg
     6121 ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat cattactcat
     6181 tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa agaagataaa
     6241 aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat tttctttatg
     6301 cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga tatcgaaata
     6361 cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc actggctgtc
     6421 gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac taaaatcccc
     6481 gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat tcgtcctatt
     6541 ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc
     6601 aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac cctatcgtat
     6661 cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc
     6721 actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa
     6781 gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc tgcttacgct
     6841 tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg ttatggaagt
     6901 cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag accacatacc
     6961 attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca tacacaacag
     7021 caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt
     7081 ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa aataaaacgt
     7141 ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa
     7201 aaaaaacgga aaacaatcac ttgttctccg ttttttgatt aatcaagcat ctaaagcttc
     7261 ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca aaatagaaag
     7321 aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca atacaccgcc
     7381 aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta aacctgtaga
     7441 agtcgcaaag taagcaacac taactaagac cattggtgtc aaaataaatg gaatgaacat
     7501 gatcgggttc aacacgatag gtaacccaaa catcgttggt tcattaatat tgaagacacc
     7561 tggcgcaatc gacaagtttg ttacaaccat atatggtttg tttttacgtc ctactaaata
     7621 aatagctaac agtaaaccta aagttgcacc tgttcctcct aagttgacaa agctgtcaaa
     7681 gaaaggtttg ttcacgataa acggtgcgct atgtccagct gcgattgcat taacgtttgc
     7741 ttcaatggct ggcgcattga ttgtttgcat caaaggatcg accatattcg ctccgtgtaa
     7801 accaaagaac cataagaatg gtgtgataaa tgcaagcagt aaggcagatg gataagagtt
     7861 tgcaagtccc ataaatggtt cttggaccac tgtatagaaa gaacctacga tatcatcaac
     7921 accgaatgca gcaaaaattg cagcaactaa agcaaacgca gagatagtga tcattgctgg
     7981 tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca ccatcaggca ttttgatgat
     8041 caatttttca tttccgctta aacgtacaaa gatttctgtt gaaataatcc caacaataat
     8101 agcaataaac aaaccgtttg agctcatacc taatgctccg cctaaagcga agaaagagcc
     8161 taaagaaatc acacctgcgg aaacaccgtc ttttcggtag ccgttagcta aatgataagc
     8221 aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag cagcagcccc
     8281 gaatcctttc catgattcac caccaaaaat tgagttcatc aattcttgga acccgggaat
     8341 cggtaagtta ttgatcatta ctgctaatgc tcctaagatc attaacggca tactcaccat
     8401 aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg ctacaggtat
     8461 aaaatgtttc tccataaatg caacaaattt atccataaat cttccctctt ttctttactg
     8521 tagattacgc gttcaatcga cgatacagat taatgatctc ttttgctaaa tctgtaaaag
     8581 ctatagatgt catcagatga tcttgactgt ggaccgtcaa aagcgtgact tcaacgtgat
     8641 ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg actaaagatt
     8701 cctctgcatc tttgattttt tcatctgcta aagtgaaatc accttttttc gcagcagcaa
     8761 tcgcttccat tgcatcgctt tttgcatttc cgccaaacat gatcagaccc atgatggctt
     8821 ctagattttt ttgatcttcc atttattgta tcctccgata atttttatta tttgtttatt
     8881 agtttttctg cttggtcgag tactttttca ccattcatca tgccatagtc agccatattg
     8941 attacatcta aaggaatgcc tttaggtgct agcttttcag caaattgatt ttgcataaag
     9001 cgtacttgcg gtcctaaaag taaaacgtct acatttttag attctagttg attatccgca
     9061 tctgaagcag atacagcaaa gatgtctgct tccattcctt ttgcttctgc tgccttttgc
     9121 atttttgtga ctaggagact tgtactcatt ccagctgaac atacaagcat gattgttttc
     9181 ttttccattt taaacacgtt cctttctata cttacacttg tatataagca agtagcgtgc
     9241 caactttaaa agcgctttaa aaagttgata tcatcacatt taaggtctat accaagttgc
     9301 atacacacta aaaaagaaag cgcacactaa acacacaaat taatttttat tacctttttt
     9361 cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt cacaactttt ttcaaataaa
     9421 cggtattcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cataagatgt
     9481 tgtttttact ttgctattct tattacttga tgcaggacag ctttttcaca acctctcaat
     9541 aaacggtgtt caatcagctg accctgggta ttagctcaaa acgctggatt cacaacctct
     9601 tttatttcaa ataaccagta aatacaatga acatctacaa aaaacttcta tttatttata
     9661 gatttcgtca ggcaaatcca attctctctt atcaatcaaa gtctgatacc acgaagcaga
     9721 ttttttcaat gttctgcttc gttcatcgtt caattcgatt cgaactaatc catatcgatt
     9781 cttaaatgca ttcattggtg acacacagtc agtgaatgcc cataacatat agccttcgca
     9841 atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa tgctgtccga taaattcgat
     9901 acggtaatca tcttgcacga ttcctttctc atccatatac tgttcttcat attctcttcc
     9961 cataccgttt tctgttatca gccaagggat attatgataa ttttctttta gatacatggc
    10021 tatatcatac atgattttcg gatagatttc ccatcccctg gaattattca ttttcttccc
    10081 aggtaaatca aagacttcat aatatcgttc aggatggaaa ggtgtctctt cattccattg
    10141 ataacgaggg gcgcgaacgc gtttaggata atattgattg attccaagga aatctactgt
    10201 atgatgctga atcgtttcta aatcctctgt tgcaggatcg aaataaatcg attgtttttg
    10261 acacaactcc aggaactctt ctgtgtagta tccttttacc atcggatcaa aaaatactcg
    10321 attataaaac aaatcataca tataagcagc tttttggtct tctggagaat tggatcgagc
    10381 atagaccatt tcaggattta aaatacagcc aatccttcca ccataatttc cgctgcggta
    10441 aatctctact gctttagccg ttgctaaaac tttatgataa ttccacatca tccacttttt
    10501 tgtgttttgt tcatgcggcc agcgaacagc atccaaatag caacgcgttt ggggaacgat
    10561 tggttcgttg aatgtaaacc agttttttac cctatcaccg taacgatcaa aggcaatttg
    10621 agcatactta gcataaaaat caactacttt tcttgaagac cagccatcat atttttccaa
    10681 aagaaaagct gggacttcat aatgttccaa acaaatcatc ggttctacgt ctgcttcgat
    10741 caaagagtcg atcatttcac ttatatgctg agcatattct tcatctacta tacagttctc
    10801 ataatcagta aaaaaacgag cccaattgat agaagtccga taatgtgtca gatggatgga
    10861 tttcatcaga tccacatcct gctggtattg ttccataaaa ttagttgcta cggctgggcc
    10921 atacccatta tgccaaacaa aatgttcatt tttataccag ctatctataa acgaatcctg
    10981 tccttctttt tttcctttcc aaccttccgt ttgccaagca gaagatgcag caccaagaat
    11041 aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt tttttagatc ccatcatttt
    11101 ccacacgatt tgctactttt acaaatggca gatagatcaa aatagaaaca ataatacaaa
    11161 caagttgcgt aataactgca ccgatatttc ctgctgttgc cagataagca ttgacgatcg
    11221 gcggcattgg ccaagggacc attacaactg ctttcgccgc aaatcctaaa gcggttgcaa
    11281 aataaccaat cgttccagtg ataagaggcg ttaagataaa cggaatagct aaaattggat
    11341 tcagcatgat cggcatacca aaaataactg gttcattgat gttgaagatc ccaggagcaa
    11401 gggataactt ggtgatttgt ttcatatctt cccgttttcc aactaacaat actgcaatca
    11461 gtaaaccgat cgtgactcca gacccgccca tactcatata catatcccaa aatggcatag
    11521 taataatgtt cggtacttct tttccagctt caaaagcttc cgtatttaca gcaattgccg
    11581 ctaaaagcaa cggttcccgt atcggcttca ccatttgatt tccatggata ccaatcaccc
    11641 aaaagacttg cgcaataaac atcaatagca aaatacccgg taacccttgt acgacacctt
    11701 ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag gtacattcct gtaagttctt
    11761 tgatgataaa tcccgcagcc gcgatacccg atacagtcag aatcgtcggg aataaagcag
    11821 aaaaactagc agaaacattc gttggcacgg tatcaggcat tctcagctgt agtttcttct
    11881 gtttcctcaa ccagcaataa agttcaatag aaaggatgga gaccaacatt cccaaaaata
    11941 accctttcgt atctgtatat tgtttagcca atacgttttc tactataaca gtctgatttt
    12001 cattcactaa ttcataaaca gttggattaa cagataaata agacatcact gcgatgatac
    12061 cagggaatac accatccact ttattcagct tggctaattc gatccctatc agaaaaacag
    12121 catagatggt caagaaactt agtgtgacat agctgatggc agatgcgata ggctttaatt
    12181 cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt ttccgagtca aagaccattg
    12241 ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg aatcaatgca gcaaatgcgt
    12301 tttttatcac catgatatac cttaaattat tgattctcgt tgccacgttc cctaaaacat
    12361 tggctaattt atccataaaa ctcattccag atcccctcat tcctgcttct tctccttgac
    12421 gtcatctctt tttgtctatt agtcagcgct tacatttcga tgaacagaaa ctatttactc
    12481 ctctttttta ccaaatataa aacaaaaatt catctgatgt atttatcata aaatacatca
    12541 gatgaactgt caattagttt ctttttctcc tattattttc tgtaattctc tccgatttat
    12601 tgccatatgg acagtcattg catcaaacgc agcaagtgca ttccgccttt cgatggcctg
    12661 aacgatctct gtatgaatat cgatcgtctc gattttcaga tgctggatat cataattttt
    12721 attaaacaaa gcaattgatt cattaatgat tgggataata tgcagcaagg ctacgtttcc
    12781 gctagcttct gcaatcatcg tatgaaaatc gatgtctaat tgaacatgtt cttgtgtgcc
    12841 ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc atttggacga tttgattctt
    12901 cgttgcatat tccgctgcca aacctgccat cttaggctcc aataaatatc tcagttcaaa
    12961 taagtctcgt atcatccgat cctgatcttc aatcaaagaa aaaccgagtg ggtcattggc
    13021 aatccctcgc ttatcactga caaatgtccc cgctccttgc cgaacttcaa ggatattcct
    13081 ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc cccacctcta aataatcgga
    13141 caattcatat tcattcggca gtttatctcc tacttgcaac tgcatttcct ttaaatatct
    13201 aaggatcttg tcagtagtta cttctactag ggttttattt tcttccaaac cacatacctc
    13261 cttttcatct gatgtatttt ttcgtaatta ttataacacg tttgaatcat ctgatgattt
    13321 tttgtataca aaattgatta atccgttcat agacatacaa acgaagaaga tatttaccat
    13381 aaaaaagact aggataaaat tcactcctag ccttcgatca tttttatttt gaacgacaaa
    13441 aaatatgatg ttcaataaca gacgatcttc attcgattct tgatgtcatc ttttgttttt
    13501 tttaatatat ggataaaaag tttgattaca tcgtttctat tctagaattt ggtcgattct
    13561 caattcttct tctggtgaga aatctaaatt atccatcatt cgaacacttt cttgtaactg
    13621 acttaaccga cttgctccga ccagtacact tgtaacagat ttttgtcgca aattccatgc
    13681 aagggccatt tgcgctaatg attgtcctcg tgatacggcg attgtttgca gtgctttgat
    13741 tttttccaga gttgatccaa cttgttcttc tgaaaggaat ggtatttcct tacgatgtgc
    13801 tctagaatct tcaggaatac catgtagata acgatttgtc agcaaccctt gagcaagcgg
    13861 actgaacgtg attgccccta gcccttcttc ttctaatact ggagatagat cgtcttctag
    13921 tagtgcacga gaaaacatat tgtagcgcat ctgatgaata atgaacggtg cttcctttct
    13981 ttttaaaatt tcagtcattt tttttgtatc ttctccatta tagttggata taccaatata
    14041 taatgctttt ccttgtctta caagttgcat caaagcttct gctgtttctt ccaaaggtgt
    14101 atcagggtct gggcgatgat gatagaagat gtcaacataa tctagcccca ttctttggag
    14161 actttggtca caacttgaaa tgatggattt acgagagccc cattccccat atggcccttc
    14221 ccacatatga taacctgctt tgctagaaat aatcagttca tcacggtagg cttgaaaatc
    14281 ttctctaaaa atacgcccaa agtttttctc tgcagctcca gcagggcctc cataattatt
    14341 cgcaaggtcg aaatgggtaa ttcccatgtc gaatgcgcct cttaaaatct ctctttgatt
    14401 atggatagga tcataatcac caaaattttg ccataatccg agagataaaa tcggtaattt
    14461 caaacctgaa tgaccggatt tacgatattc cattttttca taacgtgcac tatctgcaat
    14521 atacaatttt tctcctcctc atcaaatctt tttaaaacgc tttctttttt ctatttttag
    14581 tttactacat tttaaagaaa aggagttaat caaaatgatt ttttcgtata tttttttgcc
    14641 atttgttttt tactcgttga tagatcagaa attgattacg tgcttgctgg atttttttat
    14701 aacgtttcgg ttgttttttg taaaattgct gatgatagtt ttctgctggc caaaacgtac
    14761 cggctggttc gatttttgtg acgattggtt ggtggaaagt tcctgaactg tctagtttct
    14821 gttttgattg ttccgctaat tctttttgtc gttcgacttg ataaaaaatc actggacgat
    14881 attgcttccc tctatcttga aattgtccaa atgtatctgt gggatcagta atttgccaat
    14941 agattgtcag caactcgcta taacttattt ctcttgtgtc ataaatgatc tccacagctt
    15001 ccacatgacc tgtgtaacca ccacttactt gatcataatt tggtttttca acatgtccgc
    15061 ccgtgtatcc tgacaaaaca gataggatcc cttttttcga ttcaaacggc tcgaccatac
    15121 accagaaaca tccccctgca aatactgcac gttcttgata gtcagcatct tgcatggcat
    15181 gcttggaaaa gtcatatttt tgctccttat caaatccttc cgtaatcaat aaataaaagt
    15241 ccattacatc aggcgtcaga ttcattcgga tagctaatgg acgtagtgtt gcttctagtt
    15301 ttgataactg ttctttaacg gatatatttt ctcttatgtc attctttgtc tgaatcaata
    15361 cttttcgttc ccaatctcgt gtattcggat tcaaaatcag attataaagt gtttgaatga
    15421 atatttgttc ctcatccata gattctcctc ctttccgaag aagacaagct agtgacttta
    15481 gctgtaattt atcataaagt gaggaaataa gcgattactt catcccccta cagatttttc
    15541 gtataaaaaa gagagaatcc ttatcgacag attctccttt ttaatcaatt tattttataa
    15601 atgaaaagtt aatttacctt ttatggaaaa ctattttaaa aaataaacat aaatatgcag
    15661 ttaatgtaac aagataagaa ataatagtcg ttactgtata acttgtgctt actttaatat
    15721 cagcaactcc tttgactccg cttatttgta ccaatccatt agcattcacc tgctccttta
    15781 caggttgatg atttatttct acattcgttc ctaaataagc atatacagga agtgtgatta
    15841 cttggctctt ctcattagtt actctatata tgatagaact accagttgtt ttataaggaa
    15901 ggtttaccca ttgactgcca atctttgctt cgtgttttat tatatgctcc ttattctcca
    15961 atgaagcgat aggagaataa tcatataaaa aataatccat tcctttagtg gtatacgcct
    16021 ctactgtttc tttgtttaca acaatatgtc ccggctgaga aattattttt tgattgaaat
    16081 tttttttact agaaacagtt aaagtgatta ataacaaaac agtcataaat aaataaaata
    16141 gttctcttgt cttattgtat cttctatttt tgaaaaatat catcgttaat atcagagaag
    16201 agaatattat ttgcaagctt aagatccgcc aaggaaattg taacaatttg gctggcgtat
    16261 tttgcagaag ataccatggc accaaactag ttgtagctag ccaagtcaaa cacgtaccat
    16321 aataaatctt tttttctatg tttgttaatt ttttaaataa aatcagggga atacataaag
    16381 ataacagacc aactagtccc attgagtaag tagttaagtc gttgttcata gcttcagtca
    16441 gactatcgaa cacattagat gcatgagcat ataaatttgt cacgctaggt ttgttgattt
    16501 tttggtagag catttgttca aacatcggat accaaaaagc actcgtcaga accaatgtca
    16561 ttcctgccgc actaaataat tttttaaata tagcaataaa atctgcattc ttgaaacctt
    16621 tagaaaccaa acgaataaaa ataaaaatga taattaatgc tgtactcatt aatgttgata
    16681 gtacatggct gtacactagc aaactcatac tcaatgctag ctttacagac ggatactttt
    16741 cttcttttcc agagatgatt tgataagcat aaaataatac agggataaca agtgttattg
    16801 cgatagcttc ggcaattgcg gaacgatagt agatgtctac tgtccgataa gcagagaaaa
    16861 tatagagaca actaaataaa aatgccgctt ttttttgttt taaaaaccgc tcgccataat
    16921 gataagctac gagtgttgta cacaagttca ataaccaaac gtaaataata tagcttacaa
    16981 tcaaattttt tgtcatccaa tataacatta cagctggaaa aaaagtcaga aagggataaa
    17041 aataatttac accatatcca gtataattga atgtctcata attaatcgga ctggtaaaaa
    17101 tagtcgataa ccccttgatt cgagataagt gaaaagaaag atctccagtg gtataaatac
    17161 tctgtgtatt tttgaaaaag atgaaagaat atatactact aactataatt aatccactca
    17221 atattactac ttttttattt attttcattc ttcttgttta tctccttttt agtcacatat
    17281 cgagaatctt tcaacttatt aggtgcaaat cgtttaaaac ttgaatacac cactaaacaa
    17341 taagtcagaa tactgatcca taaagccatg agagcagttg gagtatattt tgttgtaatt
    17401 tctatcttat tttctccagc atttacatcc acattcacta agccatgttt tttataagag
    17461 tcatttacga ctttaccgtt tacttttacc tgtgtaccta aataagcata tactggtact
    17521 gtcacttttt gctttttgtt gcttgccact gtgtaagtaa tgttattatc tgcagcttta
    17581 tacttccctt tttcccacga ttctcctact tttacttcat gtttttctaa gtgaggcctt
    17641 tcttttatcg cttctgttgg ggcgtaatcg tataatcctc ccatatctga tgttgtataa
    17701 cgtgggagtg tttcttcatt gataacaata tgaccgtgtt ttgattggat aacttttgcg
    17761 taattttctt ttgctgctat agacgtgacg agtagaaaca ccacagtagc gccaatgatt
    17821 aaccatgaat attttttatt catagtcttt ttcataaaaa caagcgttat gatcattgaa
    17881 gaaaaaaaga tttggatacc taagatacgc caaggaaact ggagtaagct tatcggtgtt
    17941 ttttgaaaca aaaaccatgg aaataaatta gttacgcata cccatgtaga aactacgcca
    18001 acatagatga ttttttcttt tttctccatt tctttcagta tgaataaagg aaaaatcaac
    18061 gcaaccaatc ctaataaccc catagtgtat gttgtaatat cgttatttaa cgcttgaatg
    18121 atactatctg aaatagaaag tgcttctagc tgcaaatctg ttttgaaggg actgtgtatt
    18181 tcttgataat tcatctgctg caacatagga taccaaaaat agctagttaa tgcacaagtc
    18241 aaaaaaacag cctttactaa attcacgacc acaaatttta tatcgttttt tatttgcttg
    18301 cttaaaaaca aacgaactat aatcatacaa gcaatcaatg cactacacaa tagtgtagtt
    18361 aagacatgag tatacacaag tagacttata ctgacagata aagcgatgac ttttttttct
    18421 ctattgaata tcaatccata agcataatat aaaacagctg ggataatcgt aagagcgatt
    18481 gcttctgcaa ttgcggatcg ataatagata tcaactgttc gataagttgc aaaagtatat
    18541 aaacaactaa ataaaaaagc agcatctatc tttttaaaaa acttttctcc ataataaaaa
    18601 ctaatcagaa tcgtacaaat atttaataac caaatatata gaacataact taatattaag
    18661 ttatttgaaa ttccataaaa aacgactgca ggaattattg taagaaaagg ataaaaatag
    18721 ttcagtccat ctccataatt gttaaatgtt gtatagttga ttggcccttc aaaaatagaa
    18781 gataaccctt tgattcgtga aagatgaaaa gataaatcac ctgttgtata aatttcttga
    18841 gcatttct
//
LOCUS       DACDKJ010000060        18741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDKJ010000060 DACDKJ010000000
VERSION     DACDKJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.060"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            257..532
                     /locus_tag="IV328_002191"
     CDS             257..532
                     /locus_tag="IV328_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938304.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="IV328_002192"
     CDS             545..2617
                     /locus_tag="IV328_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8938305.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="IV328_002193"
     CDS             2782..4668
                     /locus_tag="IV328_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8938306.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="IV328_002194"
     CDS             4680..5627
                     /locus_tag="IV328_002194"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8938307.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="IV328_002195"
     CDS             5647..6174
                     /locus_tag="IV328_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8938308.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="IV328_002196"
     CDS             complement(6237..6890)
                     /locus_tag="IV328_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8938309.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="IV328_002197"
     CDS             7022..7864
                     /locus_tag="IV328_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8938310.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="IV328_002198"
     CDS             8022..8918
                     /locus_tag="IV328_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8938311.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="IV328_002199"
     CDS             8921..9634
                     /locus_tag="IV328_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8938312.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="IV328_002200"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="IV328_002200"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8938313.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="IV328_002201"
     CDS             10169..10396
                     /locus_tag="IV328_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8938314.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="IV328_002202"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="IV328_002202"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8938315.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11724)
                     /locus_tag="IV328_002203"
     CDS             complement(11182..11724)
                     /locus_tag="IV328_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8938316.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="IV328_002204"
     CDS             complement(11961..12359)
                     /locus_tag="IV328_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8938317.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="IV328_002205"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="IV328_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8938318.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="IV328_002206"
     CDS             13472..14239
                     /locus_tag="IV328_002206"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8938319.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="IV328_002207"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="IV328_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8938320.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="IV328_002208"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="IV328_002208"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8938321.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="IV328_002209"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="IV328_002209"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8938322.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5093 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcgttttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacatcgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt t
//
LOCUS       DACDKJ010000061        17889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDKJ010000061 DACDKJ010000000
VERSION     DACDKJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.061"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(45..1511)
                     /locus_tag="IV328_002210"
     CDS             complement(45..1511)
                     /locus_tag="IV328_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8938323.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IV328_002211"
     CDS             1898..2494
                     /locus_tag="IV328_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8938324.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IV328_002212"
     CDS             complement(2552..3034)
                     /locus_tag="IV328_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938325.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IV328_002213"
     CDS             complement(3140..3337)
                     /locus_tag="IV328_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938326.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IV328_002214"
     CDS             3632..4390
                     /locus_tag="IV328_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8938327.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IV328_002215"
     CDS             4450..4878
                     /locus_tag="IV328_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938328.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IV328_002216"
     tRNA            4924..4996
                     /locus_tag="IV328_002216"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IV328_002217"
     CDS             complement(5146..5517)
                     /locus_tag="IV328_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8938329.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IV328_002218"
     CDS             5732..5917
                     /locus_tag="IV328_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938330.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IV328_002219"
     CDS             complement(6000..6188)
                     /locus_tag="IV328_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938331.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IV328_002220"
     CDS             complement(6212..6475)
                     /locus_tag="IV328_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938332.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IV328_002221"
     CDS             6633..6890
                     /locus_tag="IV328_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8938333.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IV328_002222"
     CDS             complement(6961..7152)
                     /locus_tag="IV328_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938334.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IV328_002223"
     CDS             complement(7293..7613)
                     /locus_tag="IV328_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938335.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IV328_002224"
     CDS             complement(7636..8070)
                     /locus_tag="IV328_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8938336.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..9043
                     /locus_tag="IV328_002225"
     CDS             8405..9043
                     /locus_tag="IV328_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8938337.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9367..9450)
                     /locus_tag="IV328_002226"
     tRNA            complement(9367..9450)
                     /locus_tag="IV328_002226"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9414..9416),aa:Leu,seq:cag)
     gene            9978..10478
                     /locus_tag="IV328_002227"
     CDS             9978..10478
                     /locus_tag="IV328_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8938338.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10668..11012)
                     /locus_tag="IV328_002228"
     CDS             complement(10668..11012)
                     /locus_tag="IV328_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8938339.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11166..12086)
                     /locus_tag="IV328_002229"
     CDS             complement(11166..12086)
                     /locus_tag="IV328_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8938340.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12273..12764
                     /locus_tag="IV328_002230"
     CDS             12273..12764
                     /locus_tag="IV328_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8938341.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12788..15163)
                     /locus_tag="IV328_002231"
     CDS             complement(12788..15163)
                     /locus_tag="IV328_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8938342.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15489..16115
                     /locus_tag="IV328_002232"
     CDS             15489..16115
                     /locus_tag="IV328_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8938343.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16138..16842
                     /locus_tag="IV328_002233"
     CDS             16138..16842
                     /locus_tag="IV328_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8938344.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17034..17867
                     /locus_tag="IV328_002234"
     CDS             17034..17867
                     /locus_tag="IV328_002234"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8938345.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         5553 a         3489 c         3015 g         5832 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattcaatt tctgatatta acctgatcta ctcaggacaa caattaacaa tcccaacaga
     8641 aggctctgca caagcagctg ctgaacctca agcagccgtt caagaagcac cagtccaagc
     8701 tgaacctgtt caagctgaac aacctgttgt acaagaaacg gttcaaacgg aaacacaagc
     8761 agcgcctgtt gctgaaacac aacctgctcc tgctgtaaca gaaactgctg ctactccagc
     8821 aagtacaagc tctgcaaaag aatggatcgc acaaaaagaa tcaagcggtt cttacacagc
     8881 aacgaacgga cgttacatcg gccgttacca attagattct tcttacttaa atggtgacta
     8941 ctctgctgca aaccaagaaa gagtagcaga acaatatgtt acttcacgtt atggttcttg
     9001 ggaagcggcg aaagcattct gggaagcaaa cggttggtac taagattcat tatttgaacg
     9061 tttaaacaca ttcaaaatat agtaagaggt catgcgattt attctttcgc atgacctctt
     9121 tttgagttta agtttagtct ttattcctta actatcgttt gccagtattt cttgtatcag
     9181 aatctataaa tctactattt gaatcaatct attaggtttt aaactgtccc accttcacac
     9241 aaattttttt tgatttattt gaatctggtt ccgttgttta aaatttttcc tatattcttt
     9301 tagataatat gttattttct ttctcatcgc tccaggtgta ccttctcttt ataaaaaaac
     9361 aaattatgcg gctaagaaga ctcgaacttc cacgaggtca ccctcactag cgcctgaagc
     9421 tagcgcgtct gccaattccg ccatagccgc atcacatctt ttagtttagg attacatgtg
     9481 ttttttgtca agaacatatt tttgttcttt ttcatttctg tacgccctcc cttatgccat
     9541 ctacacaaat aattcatccg agcaactcca tcatctattg agggttggtg aaaaaatctg
     9601 ttttatccat atgatcgatt tcaatgttaa acgaacgact ctgggattag taactatttt
     9661 atttttttct tcatttcttt tttcctcttt ttctcgattc agctatcttc tttcatttgg
     9721 atattgatta tttcttctgt tgattactat ccttctggaa ttctagacgc tgaagtaatt
     9781 cacgttttct ttcttctaga gatcataagt aacgaatgaa cgaacttcaa ataatttttt
     9841 tattgtaatt catatttgta ttgttcgatg attcttaata cgatatgaca caataaataa
     9901 aaataggcat ggctatttac aacaatagtg ctatagtaga aaacaaatga ataaggttac
     9961 ttaaagaggt gaatacgatg aaactgatca aactatggga agcagattta aagaaagctt
    10021 atcagctaca aactagcttt ggaccaaatg agagtggatt tatcaacaat gcttatggct
    10081 atacatttgc tgaattttta gattatgtaa aaaaatgcga aaaaacgtct ttaggaatcg
    10141 atcttcctga gagttacgtt cccgcaaccg tctttatttt agaaagtgat gaacatgagt
    10201 atgtaggaat ttttaatttg cgccataaac tgaacgaaac attaagaaac ggcgctggcc
    10261 atattggtta cggcatctcc cctaattatc gtaaaaaagg atatgccaca caaggattag
    10321 cattagtact acaagaggca aaaaaaatcg gcattgatga ggcacttttg tctgtaaata
    10381 aaaaaaatct tggcagtctt aaagcacaac aaaaaaatgg ggctgcgatt tatcgtgaag
    10441 atgagaaaga atactatacg aaaatcaaac tgatgtaaaa aagaaggatt ccaaaaggaa
    10501 agggatttga atgtccttta tcaatagaac acatttattt aattaactta taatatagca
    10561 gacaaaattt tataaacaaa gaaacctttt atctcttttc ttctagaaag gataaaaggc
    10621 ttttttgttt ttcctatttg cttctcatag ctagtcagcc aagatttcta tcgtatcccc
    10681 aacgcaatcc gtgcataacg gctcatctta tctgtcgtcc atgctggata ccagactagt
    10741 ttgacttctg ctttcgttac ttctggcact tcttttaatg cgtcatggat agattctgtt
    10801 aaaatatctg ctaatggaca gcccatagtt gttagagtca tcttaatgac tgtttctcca
    10861 ttttcaggat tgaattctat atcataaatc aagcctaaat tgacgatgtc gattcctaat
    10921 tcaggatcaa tcaccatttc caaggcagca agaatacgtt ctttgattag ttctatttct
    10981 tgtccagacc gttcttggtt tgcttcgctc atcgtacgac actccttaac acgattttta
    11041 tttatcatac tcttttttta gctgcttgta aatgagaatt tagaagtaag attctcgttt
    11101 aaatttataa aaatgttctt attagatgta atagattaaa tttattctaa aataaacctg
    11161 attcctcatt ttttttgtga acgatttttt atttctggca atacgccagt tgttcgatac
    11221 attttttgtt cgttttcatt tgcaaaccgg ccaaatactg aatgagttgg taaaatattg
    11281 ataaatgcat cgtcgtcgat ttcatgaacg atctgatcaa gatcatacat ttcgtagcgg
    11341 gtgatgacca tcatgatcat gttcccttgt ttttttgaat agccaccgac agacggcagt
    11401 aatgtcattc cacgtaccat ccgttttgct agttcttctg ttacctcttc tggcttgacg
    11461 gtaacgatca ttgctgttac tttttgatga ctcgtgtgga tggcatcaat tacttgagtc
    11521 attgcataaa tggaaataat cgtatacaag gcactttccc agttaaagaa gaagcctgcg
    11581 atgactacaa tgataccatt tagaatcagc atgtagtttc cgactgtttt tccagttgtt
    11641 tgtgaaagta caagagagat gatatccatc ccaccagttg taaagcccat ttttaaagat
    11701 aatcctacgc caagacctaa taaaacaccg cctactaggg cattcatcaa aatattatca
    11761 gtgatctgtc cgactggaag cagcatcgtc atcaccgata cacatacaac attcacaaaa
    11821 ctaaggatcg ttgctcgttt tcctactttg agaaacccta atacaaacac agggatattc
    11881 aaaagaaaaa taaatgttcc tgtgtctatc ttgatatgaa accattcata taaaagcgag
    11941 gaaatgattt gagcgatccc attcatccct gcagaaaaga cattggctgg aatcaaaaaa
    12001 taatttaaag cgaccgcagc ggtcaatccg gttatcaaaa taaccactat tttctttaat
    12061 ctttcatttt tatgatacgc agtcataatt ttactcacaa gcacctctcc cctactttgg
    12121 tattccaaat gtaagaataa cagtttatct attaaaataa aatacttcga actcaaatga
    12181 gaagaatttt agcaaacagg taagaatctg ccctaatcct atgataagat agatgtatgg
    12241 taaatattta tatgtatatc aggagggttc ctatggcaaa agatgctagc ttcgacattg
    12301 tatcagaagt caatacagaa gaagtaaaaa atgcgattca gcaaacaaca aaagaattgt
    12361 ccaatcgctt tgacttcaag gattctacag tagaaatcaa attagagaac agcaatctag
    12421 ttgtattgag cgatgatgat ttcaaaatcg aacagatcaa agatgtatta tttggtaagc
    12481 tgatcaaaag aaatgtgcca atcaaaaata tccatttttc cgaatcagaa cacgctctag
    12541 gtggcaaagc tcgacaaact gccgatttgg tcaacggaat cgatcgtgaa aacgcgaaga
    12601 aaatcactac agctatcaaa aatgaaaaac tgaaagtaaa agcacaaatc caagaagatc
    12661 agattagagt gacaggtaag aaccgtgatg atctacaagc tgtgattgct ttattacgta
    12721 aacttgactt accaatcgag ttgcaattca ctaactatcg ttaatcaaaa aacgaggctg
    12781 acgtctgtca ttcctcgttt ttttgattta ctgcttcttt tgataaccaa aaacgctgta
    12841 ttccttcttc caattcccca acacttcgta cttctcttgc tgtttgccaa tgttgtggga
    12901 ataaacgtcg ataagaaaaa ctagaaaatc gttgatctgc caatacaacg attccttgat
    12961 ccgccatgcc acgaatcact cgaccaccag cttgcaagac tttgttcatt ccaggaagct
    13021 gataagcgta agcaaatcca aggtgttcct tctcatcata atatgatttg atcagttcct
    13081 gctcatgatt catctgcggc aaacctacac ctacgatcat actgccgatc aatcggtccc
    13141 ctctcaaatc gattccttct gaaaaaatcc cgccaagtac gcaaaatcca atcaatgttt
    13201 cagcgggatt tgtttgaaat tttgttaaaa atcgttcgcg ttcttcctca ttcatgtccg
    13261 tttcttggat tagcacattg ctgtcgggat attgctgtct gaatgcttcc accgcaagat
    13321 ctaaatattg ataagaagga aagaaaacga aataattccc gacctttcca tcagcaaaag
    13381 catgaatcgt ctctactaat tttgccagac tgtgttccct ttttcgataa gtcgtctcta
    13441 ggtaatttgc gatgaaaact tgctggtttc cttctgggaa aggactcggc agtttgtaag
    13501 ccagactttt ttgtcccccc agtgtttcct gataataaga gagtggtgaa aaactagcag
    13561 aaaaaagaat actgcttttt cctttatcca acgactgctc aagaaacaag cttggatcaa
    13621 tacaaaactc ttttactatc agatcgcgat acgttttttc gactgttgtt tcgtagtgat
    13681 catcataaaa ttcactgacc ttcaaaaaat gaagcaaatc aaaataataa ggaagcaatt
    13741 gttcttgtga tgggtgctca ggaaattctg ccagccattc tttaattttt tcgcttaatt
    13801 gataacctgc ctttactaaa gattcagccg gtgctttttg atgatgataa tcccaatgat
    13861 cttcttttgc tatttctcgg atcgaatcaa attcttttaa tagtttgttg aaacggcgat
    13921 gaagtttttt gaattccttt ggtatagctt ccttacttcg tttcgttttt tcataagata
    13981 gttctgccga atacatctct cgggaacgat tcaccaaatt atgtgcttcg tcaatcaaaa
    14041 acagatagtc ctcgtttttt tcatcttcaa aaaaacgtct taaataaaca gttggatcaa
    14101 ataaataatt gtaatcccca atgattacgt cacaccagat actgacatct aatgatagtt
    14161 cgaaaggaca aagtgtatgc tttcttgcat aagtttcgat gacttcacga gtgatctgat
    14221 tctcatgatt caaaagatcc cataaacctt cattaatccg attgtaataa ccattggcat
    14281 atgggcactg atctggatta caggtcgtct catctaaaaa gcagatcttg tctttcgccg
    14341 tcaaagtgac actttttact tcagctcctg tatcttttaa tgctgtcata gcatcttcag
    14401 ctacttgtct agttattgtt ttagccgtta gataaaagat cttttctccc tcttcttccc
    14461 ctacagcttt caaagcgggg aataacgtag agattgtttt tcctgttccg gtaggtgctt
    14521 ctacaaacaa tttctgtttc gtacggatcg ttttataagc agctaccgct agttcacgct
    14581 gacctttcct atactcatca tgggggaatt tcaaagccat caaagaggca ttgcgtacag
    14641 tgcgccagtt ttcttgaaaa acaagccatt ctttataatc ttcagtcagc tttttaaaaa
    14701 aagtttctaa ctgctctctt gtctgatgtt caacctttct cgtgatcact tcttctgccg
    14761 tctggaagta agtcaattgc agatttattt cttgtaaatc atgttcttga ctatagatat
    14821 aggcatacac ccttgcttga tgaaagaaaa gttccacttg ttctggttct aaatcttcaa
    14881 atcgtggttc ggaggttttg atttcatcta tataataaat accgtctctt ttgaaaatac
    14941 cgtctgcacg gccttcaacg atcaggtaat attcatctaa ttgaactgtt gtttttaaaa
    15001 agacttcttt ttcatagttt tctcccgcat ctttttggag cttgcggtgg attctcgctc
    15061 cttctaatgc agtatggtta ctttttttac gattgtcgat acttccacgt cgcaaaataa
    15121 attctactaa gtgccgcaca gatatttttg actgtttgat catttccacc acctctttct
    15181 ctctaagatt acctgaaaaa acctagttta gcaaaaagag ctctctttcc aaacataaaa
    15241 aaacaccctg ctgattaggc agggatatag gatatagaat ttggattttt ggatgaatct
    15301 aatgtaacaa ggacatatta aaatgctatg aaaacttact ttccttctct ttagaatatc
    15361 caaatacttt tcatatagtc tgataattca gataagtaaa gagaaataag tacctgtttt
    15421 tgtttcctag caatgaatgc gttatactaa tggatgaaag aagttttggc tatcaaggag
    15481 gaatgcacat gcatatcgaa agattaaaaa caggaatgat tgaagaaaac tgttatttgg
    15541 tctacaacga agaagcactt ttgatcattg accctggaga agatgcggaa cgaatcaaac
    15601 aagaaatcaa aaaaacaaac caacagccgg tagcgatttt gcttacccat actcactacg
    15661 atcatatcgg tgcagtggaa cctttacgcg cgtattatca aatcccagtt tatgtcagcc
    15721 cactagagca aaaatggcta ggtgatccga ttttgaatct ttctggactc ggtagacatg
    15781 atgatattgc tgatgttatt gtctcgcctg cagaatatga atttgaaatg aaaacttata
    15841 ctttaggaaa catgtctttt cgcgtcgtcc ctaccccagg gcattctata ggaagcgtca
    15901 gctttatttt tgatgatttt gtcgtttctg gcgacgccct gtttagagga agtatcggta
    15961 gaaccgatct gtatacaggg aatttagaac aattacttca tagtattcgg acccagctgt
    16021 ttgtattacc tgatgaattt gcagtctatc caggacatgg cgatgcaacg acgattgaac
    16081 aggaaaaaag gacaaatcct tttttcaatc gttaaaacga cttaggagga gaataaaatg
    16141 acagaaacta cactttatat cgtacgacac ggaaaaacga tgttcaatac aattgaacga
    16201 gtacaaggct ggtgtgatac acccctcaca aagcaaggac aagaaggcat tcattattta
    16261 ggtcgcgggt taaaaaatgt cgacttttca tttgcttatt ctagtgacag tggtcgtgca
    16321 atcgaaactg cccgtattat tctaggagaa cattctaagg gaaaagaaat tccttatttt
    16381 attgaaaaaa ggattagaga atggtgtttc gggtctcttg aaggtggata tgacatggaa
    16441 atgtggggtg tgatccctcg tgtattgaat tttaaaacat atgatgaaat gtttaccaca
    16501 gaagtttctt ttgaacaaat tgctaatgct gtatacaact tagataccgc tgggtgggcg
    16561 gaaacttatg aaacattgcg agaccgtgta tggacaggat ttgaagatat cgcccatcac
    16621 tgcgaaaaaa atggtggtgg aaatgtttta gtcgtatctc atggtttgac gatcgccttt
    16681 ttgcttaatc tgatcgatcc taaacagcct gttcgtgctg ggcttttaaa tggcagcgta
    16741 acaaaggtca cttatgcaaa cggtcgattt tctatccacg gcatcaatga tacgtcttat
    16801 atcgataacg ggaaaacaca tagtcgcgac tcgtttctat aatggggata ttttaatttt
    16861 taccagagaa tcataaaagg caaagtctga acgagggctt tgtcttcttt tttctcttcg
    16921 tgtacttatc tgctttattt ctcaagtttt tcttaatatt aaaaaacaga ttgggtttct
    16981 caaaaaataa cggtatacta taagatgtat cttaattggg aaggaaggaa tttatgtttt
    17041 ttgcaaatat tatcaaagca gtgattctcg gtattattga aggaataacg gaatggctgc
    17101 ccatcagtag tactggtcat ttgatcctag cagatgaatt catcaaactg gatgccagtt
    17161 cacaatttat gtcgatgttc aatgtcgtta tccaattagg ggcgatcttg gctgtcgtcg
    17221 ttctttattt tcacaagtta aacccatttt ctccaagtaa atcaacggta gaaaaaaaag
    17281 atacgtggac actttggttt aaagtgatcg ttgcttgcat accagccatc atcattggtt
    17341 tgcctttgga tgattggtta gaagcaaatt tccacaagtt cttaccagtt gccattgttt
    17401 tgatcgttta cggtattgcc tttattattg tcgaaaaacg caacaaaaac aaaacacctc
    17461 gttggacttc tctgaatgac tttacgtacc aagcagctct tcttgttggg ttattccaag
    17521 tcttggcact tgttcccgga acatcccgtt ctggcgcaac gatcctagga tctatcttga
    17581 ttggtgcttc gcgttttgtc gctacagagt tttcattttt cttagggatt cctgtcatgt
    17641 ttggcgcaag cggcttgaag atcgtcaaat atttagctga agggaattca ttcaaaatgg
    17701 aagaaactgt tgttcttctt gtaggaacat tcgtatcttt tgtggtttcc gtcttagcta
    17761 tcaaattctt actaagctat ttgaagaaaa acgactttac cgcttttggc tggtatcgta
    17821 ttatccttgg tatcattttg attggttatt ggtttattgc aatgtagaaa aaaccagaac
    17881 acttctctt
//
LOCUS       DACDKJ010000062        17325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDKJ010000062 DACDKJ010000000
VERSION     DACDKJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.062"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            44..250
                     /locus_tag="IV328_002235"
     CDS             44..250
                     /locus_tag="IV328_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938346.1"
                     /translation="MKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(612..851)
                     /locus_tag="IV328_002236"
     CDS             complement(612..851)
                     /locus_tag="IV328_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938347.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1137..1451
                     /locus_tag="IV328_002237"
     CDS             1137..1451
                     /locus_tag="IV328_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938348.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(1885..2251)
                     /gene="ssrA"
                     /locus_tag="IV328_002238"
     tmRNA           complement(1885..2251)
                     /gene="ssrA"
                     /locus_tag="IV328_002238"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2422..3195)
                     /locus_tag="IV328_002239"
     CDS             complement(2422..3195)
                     /locus_tag="IV328_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8938349.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3339..3689
                     /locus_tag="IV328_002240"
     CDS             3339..3689
                     /locus_tag="IV328_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8938350.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3670..4386
                     /locus_tag="IV328_002241"
     CDS             3670..4386
                     /locus_tag="IV328_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8938351.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            4386..5834
                     /locus_tag="IV328_002242"
     CDS             4386..5834
                     /locus_tag="IV328_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8938352.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(5904..6413)
                     /locus_tag="IV328_002243"
     CDS             complement(5904..6413)
                     /locus_tag="IV328_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938353.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(6403..7128)
                     /locus_tag="IV328_002244"
     CDS             complement(6403..7128)
                     /locus_tag="IV328_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938354.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(7351..9471)
                     /locus_tag="IV328_002245"
     CDS             complement(7351..9471)
                     /locus_tag="IV328_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8938355.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9701..10879
                     /locus_tag="IV328_002246"
     CDS             9701..10879
                     /locus_tag="IV328_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8938356.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10895..11653
                     /gene="aroD"
                     /locus_tag="IV328_002247"
     CDS             10895..11653
                     /gene="aroD"
                     /locus_tag="IV328_002247"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8938357.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11676..12161
                     /gene="ybaK"
                     /locus_tag="IV328_002248"
     CDS             11676..12161
                     /gene="ybaK"
                     /locus_tag="IV328_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8938358.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(12232..13485)
                     /locus_tag="IV328_002249"
     CDS             complement(12232..13485)
                     /locus_tag="IV328_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8938359.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13603..14952
                     /gene="gdhA"
                     /locus_tag="IV328_002250"
     CDS             13603..14952
                     /gene="gdhA"
                     /locus_tag="IV328_002250"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8938360.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            15064..16410
                     /locus_tag="IV328_002251"
     CDS             15064..16410
                     /locus_tag="IV328_002251"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8938361.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16718..17104)
                     /locus_tag="IV328_002252"
     CDS             complement(16718..17104)
                     /locus_tag="IV328_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8938362.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(17153..>17325)
                     /locus_tag="IV328_002253"
     CDS             complement(17153..>17325)
                     /locus_tag="IV328_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8938363.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         5684 a         3032 c         3443 g         5166 t
ORIGIN      
        1 aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtggt
       61 tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga
      121 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
      181 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
      241 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
      301 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
      361 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
      421 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
      481 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
      541 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
      601 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
      661 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
      721 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
      781 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
      841 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
      901 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
      961 catatatgga gagggtgata tcccagttca aaccagatac attggttacc atctgtatta
     1021 acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg
     1081 atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga tagcttttgg
     1141 atttttacag taattttatc ttaataatag caatattatt acttctaaat atttggtttt
     1201 ttgataagag tagaaatgca gggataggtt ttagaaccaa acgaagtacg tcttctgaaa
     1261 aaaagtgggt atattcacag actatttttt acggaggggt tatttcaatc agtttacttt
     1321 cttcaacact ttattctttt aatgttatag acgtttccat gtcaaatttt atatctatta
     1381 ttggaatcct tatttctgca attattacac aactacttct tgtgtttgaa gagaagtcaa
     1441 aaaataatta aagcaattat gccttttagt acaggaatta ttacttctta caaatgtata
     1501 gcgaatgtga agcctgctgt tttaattgtt ctagaaaaac ttttgagtga acaattattt
     1561 tatgttagga tgaattaatt tctctagagt ttataattaa aaaacttatt tgaaggaaca
     1621 tgcttaaacc ataattgtgt ttttccttcc atcagcataa aagttcacct caaaaagagt
     1681 gtacgaacaa aagttctttt tgtaaacctt aaaatagatt ttcctgaata cgtattgggg
     1741 atttctaaat gtttgaatgt ttttttgaat gcaaaatcaa tgcaaaaaat tttgtatttc
     1801 tctacttttc tctccttgac caaaataaaa aaacgttgat ttaacaacgt ttttcataca
     1861 tgtttatctg tctcaaccat acatatatgg agacggcggg agtcgaaccc gcgtccaaac
     1921 acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca ctttacagct
     1981 tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa cttacagacg
     2041 gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg ggcgatgccg
     2101 agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt tcgtttttag
     2161 cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc aattcaagct
     2221 cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac gtgtattttt
     2281 catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga atcatatccc
     2341 attatttata taataactgc ccctttctta agaaaaaata agcgattttc ttaagaaaag
     2401 aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc
     2461 gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt cataggcgtt
     2521 gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg gaccatcgcc
     2581 aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt tgacacctgc
     2641 cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc gataaaaagt
     2701 tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga tcagcaacat
     2761 atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg aatgaatgaa
     2821 gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct gatcgttata
     2881 gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac attcttctga
     2941 agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac cattcatggt
     3001 gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt ctgtacgtcc
     3061 tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt ttgccacttc
     3121 gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga aagtgattgc
     3181 acgataattt gacacaaata aaactccttt tttattggaa taagtttatc ttaacaaaga
     3241 atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt
     3301 tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc agtattggta
     3361 tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga aatctgcctt
     3421 tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa
     3481 agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc ggctattatt
     3541 taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta gtatttccaa
     3601 ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg gaaactggag
     3661 ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga aatgatgcag
     3721 atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa agaagggctc
     3781 gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct gaatttgccc
     3841 acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac tgtgccgatt
     3901 atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga gatgggggca
     3961 gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat gaaagctttg
     4021 catcgccgta gtgagttggc agaagcaaga gaagaagcag cagtttcaga tgatcatttt
     4081 gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta tctggacaac
     4141 cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa
     4201 ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta tcagtacttt
     4261 ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc
     4321 ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga ttctggtgtt
     4381 gcctaatgcg ttatttgtat cagcaactct tagctttttg cggaatggtc gcgttgatct
     4441 tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa gaaaacaatt
     4501 accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc tatgccgata
     4561 cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg acagagcaag
     4621 tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt gtcatttatc
     4681 cgactaatac agctttactg aattttacct taacagctga tcaatggaaa aatctgagga
     4741 atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca caggcgacct
     4801 cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt gtagttagtc
     4861 aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg ttcaaaggat
     4921 ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca ttccaagtta
     4981 agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat tttgatgtac
     5041 agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc aataaaatga
     5101 cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat
     5161 ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac ggacttctgg
     5221 aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt aaattgatga
     5281 aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc
     5341 gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata
     5401 ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca ttgaaagccg
     5461 ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg gtcaatatta
     5521 ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta gaaaaaggct
     5581 atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa caacaaatga
     5641 aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca aaatatggtg
     5701 aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa ggaaaattag
     5761 aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa
     5821 tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc tataccgctt
     5881 gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat ataaggactt
     5941 tgagcaatag tagcatcatg agtaaagagt aagatacctt gattttcttc atgagctaaa
     6001 taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc aaatgtatct
     6061 tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag ctttactttt
     6121 tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat atcaagtttt
     6181 tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa caggacatgg
     6241 tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc aaaattatcc
     6301 agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg tttttcgaga
     6361 ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt cattacgtcc
     6421 aggcctccta ctttgcaaat gatggataaa aaaagtgaaa gggaatagac taagaatgag
     6481 acagcatcca ctgagcccaa taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa
     6541 aagatcattt tggaaatcga tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc
     6601 gcttaccgaa gtcaagatat cacggatatc tggcagttca gtagtcttta agacaagaaa
     6661 attgatcctt tgaagaagcg cttcataaac tgactggaag cttacgatcc acaataaaac
     6721 caagatacaa caaagaaaga gaggaaagaa cagctgccag aaaataaaaa ggaataattt
     6781 ggttttagaa agaccagtca ggcgccaatt gatcaattcc tgcttgtttt gcctgactga
     6841 atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa aataaactga atatgccttt
     6901 tatataaaat cggctagcta attctaaagc cggaagaagc tctgtagatg cagaagcgcc
     6961 aataaatgaa agccgatctt ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt
     7021 caggcatgct atcaataaaa aagcagaaaa gcttgaaaat aaacaataga gtattgtctg
     7081 ctttttgtaa taaattaatc gagcagtagc atagcggatt attttcatac ttttcacctc
     7141 tataaaaagt atagaaaatg aatatgaata aagtatgaaa ttcctttagt gaaatcacga
     7201 attaaaacca gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg ccctattcta
     7261 ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa agaggagcag cgacaataat
     7321 tttgtcgctg ctccttttaa atttattttg tcattcggaa gaacttgtgg tcgttccgtg
     7381 aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact
     7441 aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc
     7501 attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg
     7561 aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt
     7621 cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc
     7681 gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg
     7741 gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt
     7801 ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta
     7861 agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg
     7921 catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag
     7981 atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt
     8041 atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata
     8101 attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc
     8161 tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga
     8221 ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc
     8281 atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa
     8341 ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc
     8401 atggaatacc gcactagtgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa
     8461 agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt
     8521 ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc
     8581 aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg
     8641 gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg
     8701 gatataaatc acattttttc ctttagcgag cccgaacaaa tcgtcatttg gtgctgcata
     8761 atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac
     8821 ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata
     8881 tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc
     8941 cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct
     9001 tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat
     9061 aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc
     9121 cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa
     9181 ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat
     9241 catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa
     9301 tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt cgattccgag
     9361 atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga
     9421 aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt
     9481 cctttctgca cttcttaatc acagaagtgt catgaaacca tgttacacag ctcacttatt
     9541 ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt
     9601 tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt
     9661 ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa
     9721 aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca
     9781 tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc
     9841 aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat
     9901 cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt
     9961 ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacggaat
    10021 cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac
    10081 gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcgt
    10141 aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta
    10201 cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta
    10261 tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt
    10321 agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt
    10381 ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg
    10441 cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa
    10501 aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata
    10561 tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt
    10621 agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat
    10681 acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga
    10741 agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa
    10801 agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca
    10861 aattaaaaat aaccattaga aggagcgatg cagaatggtt tgggtaaagg gattgaatat
    10921 aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt
    10981 acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca
    11041 ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat
    11101 gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga
    11161 catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga
    11221 tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat
    11281 caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga
    11341 ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact
    11401 ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc
    11461 aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc
    11521 tcgtctggct ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc
    11581 tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa
    11641 tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa
    11701 cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt
    11761 gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc
    11821 gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac
    11881 cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag
    11941 aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc
    12001 ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac
    12061 aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct
    12121 gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa
    12181 gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct
    12241 ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc
    12301 gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt
    12361 actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga
    12421 tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa
    12481 atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc
    12541 aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt
    12601 tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg
    12661 gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa
    12721 acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat
    12781 cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa
    12841 agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc
    12901 tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc
    12961 agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg
    13021 gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc
    13081 tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc
    13141 gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa
    13201 atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt
    13261 actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt
    13321 gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt
    13381 tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc
    13441 gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg
    13501 aaagtgtagc atatctgaag aaagaaaaca gaaaaaaaat gttttgagcc aaaagagaaa
    13561 atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa
    13621 tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga agaattttta
    13681 caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca tcccgaatat
    13741 atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca atttcgagtg
    13801 ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat ccaatacaac
    13861 tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa tctaagtatc
    13921 ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct gccaatcggt
    13981 ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga gatcatgcgt
    14041 ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat tgatgttcct
    14101 gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga atataaacga
    14161 ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg gggaagtaag
    14221 atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct attgaacgaa
    14281 gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt
    14341 tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc agattctagc
    14401 gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt caaagaaatc
    14461 aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta tcatgctgga
    14521 gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat
    14581 gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa
    14641 ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg
    14701 tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt agaaatgagt
    14761 cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc
    14821 atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat
    14881 ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa
    14941 ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt ttatttgctt
    15001 gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata aggaggagaa
    15061 caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa cgaacaagag
    15121 ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga aggtactggc
    15181 gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa agaagaattt
    15241 gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt agttgttatc
    15301 ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa tcacacattc
    15361 ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc cggaaattca
    15421 atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga cttttcagta
    15481 aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg tgtattcaaa
    15541 gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta cgctacaaca
    15601 gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga aactttcgtg
    15661 attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt gttaccaatc
    15721 gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca
    15781 tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt
    15841 ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg aatgcaatac
    15901 ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca aaaaggcatc
    15961 tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt catccaagaa
    16021 ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca
    16081 attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa agaagtggac
    16141 ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct
    16201 aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac aatgtacttc
    16261 tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt cgaccaacca
    16321 ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg ctttgaagat
    16381 cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga tttaaataat
    16441 cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg tactcaaaca
    16501 tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca gtcccttgtt
    16561 tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt aagaacagtg
    16621 aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc
    16681 actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct aatttttctt
    16741 ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc cgcttttcgc
    16801 ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca gagtcaccag
    16861 aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa gcatctacgt
    16921 tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca gtttctgcag
    16981 gcattggagc tttgactacc gcataataat ctttacctac ataagtagac tgatagtagt
    17041 cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct aaaatttttt
    17101 tcatgaaaga tctccttttt tatttttatt atacccgtgg tgctagttaa ttttaatatc
    17161 ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat agaacactgt
    17221 aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt gagttgaaat
    17281 tttgacaacc taatttttct aaggaagaaa aaacagtctc aactg
//
LOCUS       DACDKJ010000063        17225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDKJ010000063 DACDKJ010000000
VERSION     DACDKJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.063"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..370
                     /locus_tag="IV328_002254"
     CDS             2..370
                     /locus_tag="IV328_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8938364.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            550..1491
                     /locus_tag="IV328_002255"
     CDS             550..1491
                     /locus_tag="IV328_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8938365.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1560..2291
                     /locus_tag="IV328_002256"
     CDS             1560..2291
                     /locus_tag="IV328_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8938366.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2435..4615
                     /locus_tag="IV328_002257"
     CDS             2435..4615
                     /locus_tag="IV328_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8938367.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4635..5117
                     /locus_tag="IV328_002258"
     CDS             4635..5117
                     /locus_tag="IV328_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8938368.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5133..5894
                     /locus_tag="IV328_002259"
     CDS             5133..5894
                     /locus_tag="IV328_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8938369.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6025..7359
                     /locus_tag="IV328_002260"
     CDS             6025..7359
                     /locus_tag="IV328_002260"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8938370.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7666..8151
                     /locus_tag="IV328_002261"
     CDS             7666..8151
                     /locus_tag="IV328_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8938371.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8144..8827
                     /locus_tag="IV328_002262"
     CDS             8144..8827
                     /locus_tag="IV328_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8938372.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8953..11598
                     /gene="polA"
                     /locus_tag="IV328_002263"
     CDS             8953..11598
                     /gene="polA"
                     /locus_tag="IV328_002263"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8938373.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11643..12479
                     /gene="mutM"
                     /locus_tag="IV328_002264"
     CDS             11643..12479
                     /gene="mutM"
                     /locus_tag="IV328_002264"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8938374.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12443..13072
                     /locus_tag="IV328_002265"
     CDS             12443..13072
                     /locus_tag="IV328_002265"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8938375.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13124..13456
                     /locus_tag="IV328_002266"
     CDS             13124..13456
                     /locus_tag="IV328_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8938376.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13768..14259
                     /gene="nrdR"
                     /locus_tag="IV328_002267"
     CDS             13768..14259
                     /gene="nrdR"
                     /locus_tag="IV328_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8938377.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14282..15655
                     /locus_tag="IV328_002268"
     CDS             14282..15655
                     /locus_tag="IV328_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8938378.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15655..16581
                     /gene="dnaI"
                     /locus_tag="IV328_002269"
     CDS             15655..16581
                     /gene="dnaI"
                     /locus_tag="IV328_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8938379.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16710..17150
                     /locus_tag="IV328_002270"
     CDS             16710..17150
                     /locus_tag="IV328_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8938380.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3008 c         3796 g         4546 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      421 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      481 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      541 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      601 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
      661 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
      721 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
      781 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
      841 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
      901 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
      961 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1021 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1081 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1141 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1201 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1261 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1321 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1381 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1441 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1501 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1561 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1621 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     1681 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     1741 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     1801 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     1861 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     1921 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     1981 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2041 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2101 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2161 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2221 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2281 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2341 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2401 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2461 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2521 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2581 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     2641 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     2701 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     2761 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     2821 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     2881 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     2941 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3001 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3061 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3121 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3181 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3241 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3301 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3361 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3421 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3481 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3541 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3601 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     3661 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     3721 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     3781 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     3841 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     3901 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     3961 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4021 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4081 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4141 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4201 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4261 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4321 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4381 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4441 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4501 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4561 cgatacgaaa aaaggtagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4621 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     4681 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     4741 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     4801 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     4861 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     4921 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     4981 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5041 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5101 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5161 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5221 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5281 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5341 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5401 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5461 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5521 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5581 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     5641 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     5701 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     5761 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     5821 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     5881 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     5941 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6001 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6061 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6121 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6181 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6241 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6301 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6361 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6421 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6481 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6541 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6601 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     6661 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     6721 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     6781 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     6841 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     6901 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     6961 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7021 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7081 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7141 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7201 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7261 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7321 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7381 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7441 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7501 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7561 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7621 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7681 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7741 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7801 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7861 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7921 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7981 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8041 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8101 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8161 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8221 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8281 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8341 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8401 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8461 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8521 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8581 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8641 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8701 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8761 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8821 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8881 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8941 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9001 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9061 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9121 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9181 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9241 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9301 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9361 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9421 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9481 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9541 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9601 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9661 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9721 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9781 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9841 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9901 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9961 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10021 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10081 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10141 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10201 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10261 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10321 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa aaaccaagaa
    10381 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10441 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10501 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10561 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10621 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10681 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10741 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10801 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10861 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10921 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10981 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11041 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11101 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11161 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11221 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11281 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11341 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11401 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11461 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11521 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11581 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11641 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11701 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11761 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11821 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11881 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11941 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12001 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12061 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12121 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12181 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12241 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12301 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12361 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12421 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12481 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12541 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12601 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12661 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12721 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12781 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12841 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12901 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12961 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13021 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13081 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13141 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13201 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13261 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13321 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13381 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13441 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13501 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13561 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13621 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13681 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13741 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13801 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13861 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    13921 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    13981 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14041 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14101 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14161 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14221 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14281 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14341 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14401 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14461 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14521 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14581 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14641 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14701 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14761 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14821 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    14881 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    14941 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15001 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15061 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15121 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15181 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15241 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15301 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15361 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15421 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15481 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15541 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15601 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15661 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15721 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15781 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15841 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    15901 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    15961 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16021 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16081 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16141 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16201 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16261 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16321 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16381 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16441 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16501 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16561 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16621 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16681 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16741 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16801 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16861 tatttgtcgg aacgattgaa agtattactc aagtagaagc agaagaagaa acgcttcagt
    16921 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    16981 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17041 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17101 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17161 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17221 atggg
//
LOCUS       DACDKJ010000064        16686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDKJ010000064 DACDKJ010000000
VERSION     DACDKJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.064"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            615..1001
                     /locus_tag="IV328_002271"
     CDS             615..1001
                     /locus_tag="IV328_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8938381.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1095..1286
                     /locus_tag="IV328_002272"
     CDS             1095..1286
                     /locus_tag="IV328_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8938382.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1800..2132
                     /locus_tag="IV328_002273"
     CDS             1800..2132
                     /locus_tag="IV328_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8938383.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2428..2583
                     /locus_tag="IV328_002274"
     CDS             2428..2583
                     /locus_tag="IV328_002274"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8938384.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2859..4199
                     /locus_tag="IV328_002275"
                     /pseudo
     CDS             2859..4199
                     /locus_tag="IV328_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4440..6056)
                     /locus_tag="IV328_002276"
     CDS             complement(4440..6056)
                     /locus_tag="IV328_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8938385.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6174..6392
                     /locus_tag="IV328_002277"
     CDS             6174..6392
                     /locus_tag="IV328_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8938386.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6587..7048
                     /locus_tag="IV328_002278"
     CDS             6587..7048
                     /locus_tag="IV328_002278"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8938387.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7241..7480)
                     /locus_tag="IV328_002279"
     CDS             complement(7241..7480)
                     /locus_tag="IV328_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938388.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7504..9027)
                     /locus_tag="IV328_002280"
     CDS             complement(7504..9027)
                     /locus_tag="IV328_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8938389.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9045..9167)
                     /locus_tag="IV328_002281"
     CDS             complement(9045..9167)
                     /locus_tag="IV328_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8938390.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9197..10180)
                     /locus_tag="IV328_002282"
     CDS             complement(9197..10180)
                     /locus_tag="IV328_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8938391.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10204..10353)
                     /gene="rpmG"
                     /locus_tag="IV328_002283"
     CDS             complement(10204..10353)
                     /gene="rpmG"
                     /locus_tag="IV328_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8938392.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10374..10751)
                     /locus_tag="IV328_002284"
     CDS             complement(10374..10751)
                     /locus_tag="IV328_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938393.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10783..10962)
                     /gene="rpmF"
                     /locus_tag="IV328_002285"
     CDS             complement(10783..10962)
                     /gene="rpmF"
                     /locus_tag="IV328_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8938394.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10977..11246)
                     /gene="rpsN"
                     /locus_tag="IV328_002286"
     CDS             complement(10977..11246)
                     /gene="rpsN"
                     /locus_tag="IV328_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8938395.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11769..13541)
                     /locus_tag="IV328_002287"
     CDS             complement(11769..13541)
                     /locus_tag="IV328_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938396.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13495..15249)
                     /locus_tag="IV328_002288"
     CDS             complement(13495..15249)
                     /locus_tag="IV328_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938397.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15359..15928)
                     /locus_tag="IV328_002289"
     CDS             complement(15359..15928)
                     /locus_tag="IV328_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8938398.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(15946..>16686)
                     /locus_tag="IV328_002290"
     CDS             complement(15946..>16686)
                     /locus_tag="IV328_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8938399.1"
                     /translation="LLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENIL
                     KDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEM
                     DYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIA
                     SLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYL
                     KNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         5392 a         3178 c         2499 g         5617 t
ORIGIN      
        1 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
       61 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
      121 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
      181 aggaattttt cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat
      241 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
      301 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
      361 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
      421 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
      481 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
      541 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
      601 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
      661 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
      721 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
      781 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
      841 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttgata
      901 aaactgataa agaaattgga aaggttctgg ggattacaag acagggtgcg acaaatttaa
      961 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     1021 tttgtatgct tctttgctcg ttctcctcct tcccaaagaa atagattata agtccatggg
     1081 aagaaggtag atttatgaat tctttaaaag aagatttcat tttagcaaag gctggaaatg
     1141 aagaagctgt tgaggcaatt ctcaaaagat ttagctcttt gattcataag caatcctgga
     1201 gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata tatctagcta
     1261 tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat aagtaaaaag
     1321 atagtttcaa atttcgaaca ataaatttga aactatcttc tttataatta taaagtatcg
     1381 attttacagc gttctataat gattcataga atctaaaacg aatgatttta attatcaacg
     1441 ttttttaatt cgtttttagc ttaaaaataa cttttgtcac agtctcactc ctaaaattac
     1501 tttatttacg tatgaagtgg gcaggaggtc gaatccagaa ctgggctatg gtacttcaac
     1561 gaggacttct cctctagaat tgaaaagcat gcgatttacc ttccgtaacg acggtagatt
     1621 tattcaacta tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa gagaattttg
     1681 gttgttaaaa actcttatct atcaacttcc acagtttagt tgacaaaccc tgatactaag
     1741 ttgggctatg atacactttt cttgactatc agttacgtta ccggaaggaa tgaactctat
     1801 tgagaaaaac atatgatcgt aaatttaaac taaagatttc acaggatatc cttgagaaaa
     1861 aagtaactac caagaaaatt gctgaagaat acaatatctc tcgtccaaca atttcccgat
     1921 gggtttcaga atatcgtcga tacgggaaaa atgcttttgc tggacaagga aaaagattac
     1981 ctgataaagc agatttctac attcttgaac aggaaaataa acgactaaaa gaggaagacg
     2041 agtgttctac gaacacaaag atcgatacgg atctccaggg atcactcaaa aactttacga
     2101 tgaaggaatc gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg
     2161 cacaagagga tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat
     2221 ggtaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt ttgggtgatg
     2281 gatattactt acattccatg taatgacggt cgcctctatt tgtccaccta tattgactta
     2341 gcaactcgta ttctacgtta ctatatggta gattcacaca tgaaaaaaga gattgtcgtc
     2401 cgtccattac aaatttatta aggagaattg cctacggtaa ttcattccga tagaggtagc
     2461 cagcatcggt cttttattta tcaagagctg ttgaacgacc accacatcac acattcagtg
     2521 agccaaactg ggactcctgt tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa
     2581 tgagaattaa tcgactccaa caaatataaa agtaaaatag agatgaaggt gttaatccaa
     2641 gattatttag aagactacta cccaaacaaa agaatccata caaaatttat gatgactcct
     2701 tgacaatttg aagcgaagaa aataagggat ctataatatc tagtgttggt gacgccttct
     2761 gttgccaata ctttttcttt tttctaataa acgcaaacag acaccttaaa atcctttaga
     2821 ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat gtcctattca gagtttacca
     2881 aagagttact agatattcta gacctaaatc ttatctttca tgaagatgcc tttcgcaaag
     2941 agcgaatcaa tgacgaaaca tgttttgttt ttgatggcac attgacctat caaccggagg
     3001 aatgctttca tcgccactat caaaatgaac aaacgatcat taaatagggc tggaaaaaag
     3061 tatccatctt attgaatgat gtcagtaact acaagaccat tctgcggatt aacaaacagc
     3121 gcttcaaatg taaacactgt ggaaagactt ttttagcaga agattcagtt agtgatcgtc
     3181 gctgctccat tgctcgcaga gtcaaacaag ccattcttga gctattaagc gaacctcttt
     3241 ctatgtcttt gatcgcacgt atgaagcaca tttctccgac cactgtcatt cgaatacttc
     3301 gcagccttcg gcctaaaacc atttctttga gccagctgtt accagaagtg gtgtgtttcg
     3361 atgaattcaa atcagtgaaa aatgtctcgg gagccatgag ctttgtcatg atggacggca
     3421 agacccatca actgattgat attgtggaaa atcggcaact caatccttta cgcgactatt
     3481 tcttgcgcta ccctataaaa gtccgtgaac aagtgcgtct agtggtttca gatttctata
     3541 caccctatcg cacattggtg aaagagtgtt ttccgaatgc ccgaatcgtc gccgatcgct
     3601 ttcatattag ccaacacatt ggtcgggctt ttaccaacca ccgaattcaa gtaatgaaga
     3661 cctttaaaaa aggggatcgt cggtcgaagc acttaaagaa atactggaaa ctacttcaaa
     3721 agaatgcgtg ggaattgaaa ggtcagcatc gctactggcg tccgtcattt cgagatcatc
     3781 tgacagaagc agagatagtc gatcggctat tatcttatga tgattcctta aagcgaggct
     3841 acgaagtcta ccaggatttt ctttccgtta tcagaagaca agatgttcct gaattcgtcg
     3901 cgctcctaaa agaagactac aaggagttgc cagagcacta ccaaccagtg ttcacgacct
     3961 tcaagaaata ccaaacagaa atcaaacgag cgttgcgcgt tccctattcc aacggcccta
     4021 tagaatgttt gaataaccat atcaaagtac taaaacgaat tgcctatggc tatagaaact
     4081 ttcaaaacta ccgagaacga atctttttat atcgaggaaa atattttaag aaaactaaga
     4141 atactaccca actgactaaa gccagaacaa caacacggct agacaagata gctgtctagc
     4201 cgtgtgagta tctcgattat tctattgaat ttcttcacca acactcattg acgaagagcc
     4261 aaaataagta atctaaccga ctgttaacta aaccattgca ggtcccgtta aagatacttg
     4321 tgcctgttgt caccgcttct ttttcttgtc cacaagcgct taacacactt aataaagccc
     4381 ctgctattat taataataat atctttttct tattaaaaac ctcctcatct atttccagat
     4441 tagtaaatgt agtaatctgt gctttctttt ttctcaatac taataactaa cttatggggt
     4501 aaacaaatac tcgtttgacc gggttttgag atccatccag ttctaaccgc gatttgatca
     4561 ggactattat cttctttaac tcgaattctt ccatctttaa tctcaacaat attatattgt
     4621 cccttcgcag gatgataatt cacgagttta tgattgatct tatccaaatc gaagcgatcc
     4681 atttccttgc catcaattcg aacgatggca tatttgatag catcttcagt tgtttcattc
     4741 tccttttcta tagagaatac aaataaggga ataaaagagg taataatcaa aatgccaata
     4801 attatgacat cgcctatctt gacaccagct tttcttaaaa atttcattaa cgatatctgg
     4861 ctaaacatat tagacttttt ccatggaaat ggtgaaattt ttcggaaatt atctgcatta
     4921 agagaatcag ttaaacaacc ctcaagttgt tccttaatac attgttgatc catcttcata
     4981 ccaataaaaa ctagtttatt aatgcgttca ccaaacaacg gatgccaggc ttccttcaat
     5041 tcgggatttt tttgtataat catttgacac tcaattttag gtaaactagc tacccaataa
     5101 tttaatgatt caatactttt atagctaccc gatgtttcaa agatcgtcga aaaatcacta
     5161 tgttgggcaa accaaagaaa cccttttgct cttgtaatat tgccaggaaa atttttatcc
     5221 gtaagatggg caaatttttg tgcgtcaagg agtaaccgat ttcgatagat aaaacttgag
     5281 ataccatatt cttctgtttc aggaatatga ttttgatagc caagctctaa ctctttgaac
     5341 cagcccactg aataggaagt ctcatctata ttaaaaagat ttgtatttag aattttcgat
     5401 aagtctactt cacctttaat tgtcctaatt attttggcat tggactgtaa cttagcaatt
     5461 agaccgttaa ttttgtaaat cgtcttggtt gacactaagt cactattatt taaaagtaat
     5521 atgttacaac actcgaattg atcaattaac aagccacgaa tatcctgttc ttcttcaact
     5581 acattacctt cagcttttct ttcctgaatc ttttcacccg tttcgaaatt atcccaaaaa
     5641 cgttttacat ccactactat aataatggca ccaatctcta atcgttgggt caaatcaatg
     5701 tctaaagggt ttttagctaa tataattgct tgaataattg gaattggttt gctgattcct
     5761 gaagcttcaa taacaatttg attcaagtca gaaaattgca ctaattgatc tagttcaaca
     5821 atcaaatctt cgcgtaatgt acaacaaatg catccattcg gcatcgaaac aagtttttct
     5881 ccagttcttt taaaaccttt cttgtcgact aataccccat caacatttac ttcccctatg
     5941 tcatttacaa tgacagcaat ttcctttccg tgatcaccgt acagaatatg atttagaagt
     6001 atcgtttttc ctgaaccaag atagccagtt aagattgtaa taggtatttt tttcataggg
     6061 aacctctttc tataccttga atttacactt taagtgcaac acataaaaat gagtcataat
     6121 cgatatccaa ctagtataag tttgtcgaga acactcctag aaggaaaata attatgagcc
     6181 agtatcgtca tcttactata ctcgaacgag aaagaatctt tctgcttcac gaagaaggat
     6241 tttctattag tataattggc aacgacattc agcatagccc gtttactatt ttacatgaac
     6301 tgagaagaaa caagaaaaaa actattcacc ttccaaagcg gaaaaaaatt atgcttatcg
     6361 aagaaaaaaa tgcggacgtt atcacattct aaatgatccg tgagtttggt caattgttcg
     6421 tcatttattt gtcgattttc agtggtcgcc agaggaaatc tcgaatcgtt tgaagcatga
     6481 acatatggac ttttcaatta gttttatcac gatctaacgt gcaatttgca atgatctttt
     6541 cgatacagaa cccctctcac acggcaataa aggcctgatc cgttcattgc accatcgagg
     6601 aaaaacaaga catacaaaaa atcatgtcga gtctcgtgga aagatttgcc ttactcattc
     6661 gatccatggc cgtccaaaat ccgcagatga tagaagtgat tttggtcatt ggaaagccga
     6721 tattgtagct ggcaaaactg gtggtacttg tttagtgact ttgacagaca gaaaatcatg
     6781 cttcctttta gcaggaaaga aggtccccag aaagaaagcc gcttatgtga gcgagaaaat
     6841 gattgaactt ttaagcagta ttccaaatga ggcggtcaaa acgatcactc cagatagagg
     6901 aaaagaattc tccaatcatt tcgcagttac agaagcattg aataatatgc ccttttattt
     6961 tccagatcct catgcccttg gcaacaatga acaaattaaa atacgaatag actcattcga
     7021 gaatacttgc caaaatcgaa agagatagct acggtagata actcaacgat aaatcagatg
     7081 attgaaaaac tgaataacag acctagaaag tgtttagatt ggagaacacc ttatgaagtc
     7141 ttctttgata aagtgttgcg cttgatttga caattcaaga cataataaaa agcagatcaa
     7201 atccaaataa gggaaaaatg atctactctg gtattatcaa ttaattggcc aagctattat
     7261 gccgttttgc taaacgtcta cttcttccag tattgacctt gataaaacaa caatcaggat
     7321 cccgttttaa tactctatga actgtgtcaa tactaacatg taaagttgca gcaatatcag
     7381 ctaatgtact cccatttaat ctcatttgct ttgcaaatcg ataacgccga taagcatcaa
     7441 tattcttgga atccatagac aataatactt ttcttaccat gtatttcctc caaaaattga
     7501 attttaatga gccatcattt cttgagcaat ttcatgttta gacaatgaag ctggatagaa
     7561 agtaggccaa ttatgcaatt cattatagag tttttcttga ctttctccac caaagaaaat
     7621 atggaaatgc tctgccttac ttggtgcaat tccatgatca ctaaattgaa tatatttaaa
     7681 acgaccagca cctgcatcat cagtttcaaa attaaaacga acaccacggt ttcctttttc
     7741 ataattcaaa attttgtatc caacatattt gtatgtgtat tgatgatgtt caccgttgac
     7801 tacaaaatca atggtgttgt ctttaatgtt gatagtatca atatctgtct tatagccagt
     7861 agtataataa tctttgtact cggcagctgt catgtcttta ttgatttttg ctttgtaatc
     7921 gaaaacttca tccaaaattc catcttttaa caacggataa acagattgcc attcgcctgt
     7981 ataatcactc aattcgcgat ccttcacatc ggcatcttta aagtacccat tagcaactgt
     8041 cttttcatcc ttcgcttctt tttctggaac tacctcttca ccagcaacca tagttgtttt
     8101 ttctaacgct tttagattgt cttccattac tgaaacataa tcttcaccat tatccatttg
     8161 ttcttttgta agactttcaa gaggattcag tacttccaat tttatcccag cttcgttggc
     8221 caaagttttt gcaattttat cattagcatt cttttcaaag taaatataat tgatcttatt
     8281 ttttttaaca tactctttta attcagctaa tcgaccagaa gatggctctt cttcaggact
     8341 tagtccagca ataggaactt gtttcaatcc ataatccaaa gctaaatatc caaaggctgc
     8401 gtgttgcgtc acaaaacttt tttgtttcgc ttctttaaga ctagtggtat agtcagcatc
     8461 caaccgtttc agtttagtca aatacttttc agcatttgtc tcaaatactt ttgctttttt
     8521 cggatacaat ttaactaact gatccttgat atttgaaact tccttaattg ccattttggg
     8581 tgaaacccac gtatgaggat ctagttcatg atggtgatct tcttctccgt gatcatgatc
     8641 ttcttcttca ctacctggca ataaaatcat gtcttttgtt ccttctacta cattaggccc
     8701 agctttcttc caactttctt tagcttttgg tacccatgat tccatgtttt cgttatgata
     8761 tacaaaaaca tcagcatcat gagcttttgc catatcctta gctgagggct catagtcatg
     8821 aggttctgat cccgctggaa ccattaaatc cacgttcgct tcatcgccga cgatgttttt
     8881 ggtgaaatca tacattggat agaatgttgt tacaatttct aattttccat ctttatcatt
     8941 cgatactttt gcatcattag tgctggagca agctcccaat aatcctagag ctgctatccc
     9001 aataagtcct acactgattt tcttcattct ttttccttcc ttaattatgc taatttcttc
     9061 attttatttc gtttatgttc tttgataatt tttaatgccc tttttctagt tttaatattc
     9121 gggcttttaa gtcgtcgata tgcactagat atatccgttt tctccattag aaattacccc
     9181 caatcactac ttatcattat ttgctttccg ccagtaattt atcaaaactc tgtttgatcc
     9241 tttcttcatc caacattttt ccgattagaa taatagtggt ttggtctttt tgtcgagtgg
     9301 tgtattgaaa attaacctgt tcgtttacct cttgcatggc aactataaaa tcctgctctt
     9361 gtaaagcaat caatccttta aaacgataca ggctcccttg gttggttaaa atcagccagt
     9421 caatccattg gagtaaaaaa tcttttttta tgtcttgatc tgaagttaag agaattgatt
     9481 taaactcatg tacttgatgg tgttgatgat agtatttgcc attatagtga ttttctgatg
     9541 aatcactttc aaaaactaag ggatgattaa attttttcaa attataaaaa tccttttttt
     9601 gaatcgtttc actatgtgtg aacattcgaa tatcggataa agggttaata cttttgattt
     9661 ctctctgtaa tttttttagt gcagataaat tcaagttttg ttttctagac acaaaaatgc
     9721 gatctgccat gacaagttgc ttaagggctt gtggttctct ttcaataagt tcaaaactgt
     9781 cactatcaat aacagtcaat aaactatcga tatatactct tcctcttagt tgaggcgctg
     9841 ttaaaagtgt ttgaatgatt ggttggggat ctgctatacc tgtagtttca atgatgattt
     9901 gtgaaattgg ttgttgatgt tcttctattg tttccagtaa agaaatcaat attgctaata
     9961 aatcactgcg aagactgcaa cacatacatc cattatttag ttgtaacacc cgttcctcgc
    10021 tatgaactaa taatttgtga tcaatcccaa cttcaccaaa ctcattttca attataataa
    10081 tttcttccct ggggtagggt gagacttgca aggcttgatt gattagcgtt gtttttccag
    10141 cccctaaaaa cccagaaacg attgtaactg gtatgcccat tttgtcacct ttttaccact
    10201 tagctatttc acttctttaa aaattgcccg tctgcgcaat tttggcgaat acttttttaa
    10261 ttctaaccgt tcagggttat tccgtttatt tttgctggtg agatacagac gttctccagt
    10321 ttcgacacat tccaaaataa tattttgacg cataaatctt cctccttcaa tacttatcga
    10381 tcttcaagat atgcttgaat ttgttgtgca tcttcaaaag ttaactcgcc agattccaat
    10441 tgtgaaacta aattttcttt catatcaaca ggtatctccc aataaccatc gatacattga
    10501 aataactcct cgtcatactc ttcaaaaatt ggatagattc ttaaaaaatc atcaaatgat
    10561 aagccttggt cctcatagta atctttgact aatccatttt caattgcctg ttgggtcggt
    10621 aaacccgttt cagtaataat tcctctctct accatcgaac gataaaaagt gcatttatct
    10681 agcgattgct gggtcgtttc aagataatac tttaatcctt cttcaactaa aggtgggtaa
    10741 ataatttcca ttttgattcc ctcctaaaag ttatatatta aactattttg aagctccttt
    10801 aattactttc tttccattat agtacccttt taaagataca tgatggctac gtctataatc
    10861 accattcgct tcgtcaaatg aaatttctgg ttttactaca gtttgatgag ttcttcttaa
    10921 ccgtttcttc gccttagatg tttttcttgc tggtactgcc atgatatttc ctccttttac
    10981 caacttgctt tttttactcc aggaatcaac ccttgatggg caagttctct aaacttaata
    11041 cgggacatac cgaactttcg catataccct cttggacgac catccgtctg atcacgaagc
    11101 ctcagacgat tcggatttga atctcttggc aatttagcga gagcggtgcg atctcctgcg
    11161 gcttttaatt cctgacgttt atctgcatac ttttcaatca tcgccatttg tttctttgct
    11221 ttagcaattt tcgacttttt tgccatatac ctttatactc cttttatttt gatttagaca
    11281 cctcaaatcg taatcgttac gttttgacac tgatacttta ccaacaagag ttgctcttgt
    11341 caacaataaa taacatgaaa taaaactcat atgaatagat aactatttga aaacgaattt
    11401 atcggtacat agagataatc aaatcattta aaagaatatg cagtttaggc tctgaaaaat
    11461 aagaattcac ttttattagt ccaatcaaac acaaataccg ttttaagtag cttttcctct
    11521 acttaaaacg gcaatatgaa agaaactata actttttatt caatctttta tgtttaactg
    11581 gtttatcgcc aattttttaa aactttgatt attccttata ttataagaat agacattaag
    11641 tgaactaatt caatcatact gattctttcg tgtattcaaa attagatgct gtttaataaa
    11701 attattcttt ttgaaaaaga attataacta tatcccaaaa attgtaggtt aatttaaagc
    11761 ctatttcatc atcgtgattg agcttctttt tcatagacga atttctcaga tagtttagtt
    11821 ttctttatga gatttttgta tatttctgac cttcgataca actcattatg agtaccttga
    11881 tcaactagtt ttccttgatc catcactaca attttattta ccttttcaat agacttcaag
    11941 cgatgcgata taattagtac tgtcttatct tgtattaaac gatttaaact ttcctgaata
    12001 cgtttttcat tttccgcatc taaactggca gtaatttcat ctaaaattaa aattggcgca
    12061 tttttcaaaa aagcccgtgc tatggatagg cgttgtcgct ctcctccaga aagtgaactt
    12121 ccgttttctc cgaccaatgt atgataaccg tcaggtagtt tttcaataaa gtcttcgcag
    12181 ttagctaaac gagctgcttt tttcacttcc tcatctgacg cttgactatt tccaatacgt
    12241 atattttcca aaatacttgt attaaacaaa gttacctctt gaaaaacaat agcgattttg
    12301 ctaaacaaag aagctggaga tacacgttta atatcatacc catcaattaa aatacagcct
    12361 tcatcataat catacagtct agacactaat ttcaagatac tagttttccc ggaaccacta
    12421 gcacctacta acgcagtcac ttctccttgt ttagccgtaa aagagatatg atctaaaatt
    12481 ggggtattgt tatcatatga gaaagagaca tctcgcaact caatgtcaaa agatttcaga
    12541 ggactatctg atccctcttg gatactggtt tccttcatag ctctaattcg ctgtatttta
    12601 ggagctaaat aaaatatttc aagcactgct tctttcatag aatcgaagga atcctttatt
    12661 tttattgcgg ctagtaagta accaacgacg tagagtatag tcacttcacc cgttaacagt
    12721 aagtgaacac ctactagaat gactactgct aaactaacat atgagaaaat agaggataag
    12781 gccatgactg aaaaagtact taattctacc tttaaatgaa tcctttcact ctcttccatt
    12841 tttttgaaaa gtctgtcttg aactttttta gatagattaa agctattaat ctcttgctgc
    12901 aattctatag tttcttgaaa ttcttccgag ttttctctca aagtatcgta atatttagta
    12961 ttggctttcg tttgggattt ctttgataac aagattaaga caaaactaaa taacgttggc
    13021 aaaataacag ctaatcccat tttgacatta ccaaccaaaa gcatcactga aataaaaggg
    13081 aaaaacagag ccataccgcc ggctttaggt attgcatgac tcatcgcatg ctcaatacct
    13141 tcaacgtcag acatgatagt ttgagataaa tctgataaat tatgttttga aaaataggat
    13201 aatggtaaat ttgacaaatc gtctgcaatt tgcactctta aatgcgcact ttctttataa
    13261 gtactattat ataagttttc atactcccta tctaacaacc aatacatgaa aagcaaagta
    13321 acaaacgata cggcaaaata aagccaacgg ggctttgtat tttctaaaac taattcgtct
    13381 atcagcatca ttataatgaa tgctggtacc atattaatgc agtatacaaa aaaactagaa
    13441 atagaagctt tagttaaagc ttctgcgccc tgatcactta aagcaaattt gtttttataa
    13501 aaacttttca ttgtcaagcc tccaatcatt tgcattctca taattagtga ccaattgttt
    13561 atacacttga tgtttagcca ttaattcatt atgatttcca cgttctacta ttttcccttc
    13621 tgacaagaca agaatttcat ctacagattt aatgctggat aatcgatgag ctatcataat
    13681 aactgttttt tctttcatca gatgttgaaa agccttttgt agttcatact cattatctgg
    13741 atcaatagat gcactggctt catccataat aacaatcttt gcatctttta aaattgctct
    13801 ggcaatagct atacgttgtt tttctcctcc tgacagataa actcctttac taccaattaa
    13861 ggtatgctct ctttcgggaa atttttctaa aatcgattcg caaccggcta atcttagcgc
    13921 gtcaaatact ttacttgctg gcgcttgagg atttgctata gaaacgttat cgtaaatact
    13981 ctgattgaat agtttcgtat cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt
    14041 tgaatagtga tttaaaggct taccaccaat tttaatctgt cccttatcca cattgtagaa
    14101 acctgaaatt agtttagcta tcgtactctt tccgcttcct gattttccta ctaatgcata
    14161 aattcttttt tgttctaaaa ctaagttgaa attttctaaa acacactctt ctttgtatga
    14221 aaagctgaca ttttcaaatg caatgtcaca atttttaatc tgtgtctcgt tcccatattt
    14281 tagttggtct tgcttcattt ctaaataaag ggagtctagt gtttcgactg catatcttgc
    14341 ttcaaacaag tgcatactta catacattat tctcatgaaa gaaacaaaaa taattccact
    14401 taaaaaaaac aacatcaaca agtcaacagc taatatcttg ggagtggtaa gagaagttat
    14461 aaaaaaggtt ataggaacta tcaaaatgca aactatgcca aaaaatagcc attgaaaaag
    14521 aacaaaaggt gttttcccac tcaaagaata cttatatgca aacttagcat aatcgttaat
    14581 tgctttattc aaggctttaa atgatgttac tttagcacca aatatcttta taacttgaat
    14641 tccgcggata tattctaccg tttcagagct taacacatct aacgattctt gatatttttg
    14701 aataaaattt gtattctctg tcattttctt taacaagaat acaccaatta ctaacatggc
    14761 gattagaacc aatccaattt taaagttaat gaaaaagcct aaggcaactg ataaaacagg
    14821 tgttactatg gcttgagcac tatctggaat caagtgagca attgccatat gtgttttagc
    14881 cgcgttgtca tcaattgtct ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa
    14941 acttgcttct gttaaaccgc taattccctt ttttctcaaa ttagtctcta agcgaaaccc
    15001 aaggatatga gaaaataagc cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc
    15061 aacagtttga accgcataac tcttagctaa atattcatct cctacaataa ttagagactg
    15121 aagaaatttg taaatactgt aatatccata gactgttaat acgacagaaa aatcagaaaa
    15181 taaagtggct aaccatccgt agtatttcat tttagggaca taactaaata attttttgta
    15241 tactttcatt ttttcctccc ccgcattcaa tttcttctct tattcgttta caactgttaa
    15301 tgctttccgt aatagcgtaa attagagatg tacaaacaga taaaaggtaa actacgttct
    15361 attttttgta tttttttaag acttttcttc caatcaatac accaatcata gataaacttg
    15421 ctacaactaa cactgaaatg attccccata tacctaccgc atagtggatc attgcatcca
    15481 ttgttgcagc agattgtcct ttatctatcc aatcttgacg aaaacgatct gtaaagaacc
    15541 atataggaat ccactgtccg gcagcactgc ctaattgcat tagagcccaa gacgcaactt
    15601 gaggaattgc tcgatcatat ccaaatacag atagaataat atcagctaag atcgccatac
    15661 taatcgaact gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa aataacaata
    15721 aaacaattgt gtaaatagta aaaattcctt ttctagggta gttatagcta atataaacaa
    15781 atatgattcc agataataat ccaaaaatcc cgggggatat gctgtgacca aacgccccac
    15841 caatactact aaccattatt gaaaaaaagt aaattactag ggaaataact gtagctaaac
    15901 caataaaaat ataatctttt gttttcatat atattctcct caattctatt tcgttatgtc
    15961 attaaaaatc tgttctatca aagttgaatc ttctactcta agttttatat ctttttttat
    16021 tccaccattt tttaaataaa taattctatt gcatgtttta taaagaaatt ccggatcatg
    16081 agtagctata actactgcac ccttttcttt taagtcttta ataaggtcag caatcctttc
    16141 catattaata taatccaacc cacttgtagg ttcatcaaat agaaataaat tagtttttga
    16201 caatgaagct atagaaacca ataatctttg tttttctcca ccggataaat caaacggtaa
    16261 ttgatctttc attttataaa gttccatttt ttttaaaatt ttttcttgct gatctttact
    16321 aactatttca ttccccaggg ctaactcgct cctcactgat tctgtgaaaa attggtaatc
    16381 catttcttgc attactaaaa acggcgatga caaatttttt ttattttcga ttatctttcc
    16441 tttggtaggg cgaataatct tcatgagtat tcttagtaag gttgttttcc ccacaccgtt
    16501 tttccctacc aaaccaataa catctccaac tttgacatca aaatttatgt cttttagaat
    16561 attttcataa gtaatttctt caacttttaa tacatctgca cttacctttt cgatataggg
    16621 aacttcaatc gaactattaa aaatgtcaaa tttccttaat gcgttattgt tttcagtttt
    16681 taacaa
//
LOCUS       DACDKJ010000065        16668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDKJ010000065 DACDKJ010000000
VERSION     DACDKJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.065"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(157..864)
                     /locus_tag="IV328_002291"
     CDS             complement(157..864)
                     /locus_tag="IV328_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8938400.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(943..1281)
                     /locus_tag="IV328_002292"
     CDS             complement(943..1281)
                     /locus_tag="IV328_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8938401.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1392..2354)
                     /locus_tag="IV328_002293"
     CDS             complement(1392..2354)
                     /locus_tag="IV328_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8938402.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2369..3700)
                     /locus_tag="IV328_002294"
     CDS             complement(2369..3700)
                     /locus_tag="IV328_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8938403.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3708..4388)
                     /locus_tag="IV328_002295"
     CDS             complement(3708..4388)
                     /locus_tag="IV328_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8938404.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4607..5086)
                     /locus_tag="IV328_002296"
     CDS             complement(4607..5086)
                     /locus_tag="IV328_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8938405.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5213..6892)
                     /locus_tag="IV328_002297"
     CDS             complement(5213..6892)
                     /locus_tag="IV328_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8938406.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7440..8942
                     /locus_tag="IV328_002298"
     CDS             7440..8942
                     /locus_tag="IV328_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8938407.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8955..9617
                     /locus_tag="IV328_002299"
     CDS             8955..9617
                     /locus_tag="IV328_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938408.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9718..9921)
                     /locus_tag="IV328_002300"
     CDS             complement(9718..9921)
                     /locus_tag="IV328_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8938409.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="IV328_002301"
     CDS             complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="IV328_002301"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8938410.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="IV328_002302"
     CDS             complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="IV328_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8938411.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13885..14499)
                     /locus_tag="IV328_002303"
     CDS             complement(13885..14499)
                     /locus_tag="IV328_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8938412.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15105..15482)
                     /locus_tag="IV328_002304"
     CDS             complement(15105..15482)
                     /locus_tag="IV328_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938413.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15782..16663)
                     /gene="galU"
                     /locus_tag="IV328_002305"
     CDS             complement(15782..16663)
                     /gene="galU"
                     /locus_tag="IV328_002305"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8938414.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4829 a         3593 c         2847 g         5399 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcccttatt ccaaataaat ggtattcaat cagctaactc tcggtattag
      121 ctcagatcaa agcgctgatt tcacaacttc tttattttaa tctcttcggt tgccgccgaa
      181 caataagatc aagcgcaaca gctgcaggaa tgtcgaaagc gctgctgcaa cataagtcag
      241 tgctgctgca aataatacgt gtcttgccat cggtacttct tcttccgtca gtagtccgcc
      301 ttcactcaaa atttggattg ctcgccgaga cgcgttaaat tcgactggta atgtcaccaa
      361 ttggaaaagt aatgctaaag aaaaagcgaa aatcccgata ttgattaacg tagagttcca
      421 gctaagcaat acacctacca taatcaaggg gaacgaaatc atcgaaccaa agttagctgc
      481 tggaacaagt gctgcacgaa tcttcaatgg gacataaccc gaagcatctt gtaccgcatg
      541 tccgcattca tgagctgcta cacctatcgc agcaactgaa gtagactgtg cagttgcttg
      601 agacaaactc aacatcttat tacctgaatt gtagttatct gtgagatctc cggcaatttg
      661 ttgaacaccg acattattta tctgttgact ttgtaatata tattgtgctg cttgtgtacc
      721 agtcacattg tttttgcttc tcacttgatc gtattttcgg aaagtactat tgacgtacgc
      781 tgacgcaatt ccggaaataa ctaatccaag aatgaccaaa agaaatgtcg gatctaaaaa
      841 accatacatt ccataaccat acattttcgt ttcactcctt ttcgtttact ttattttatc
      901 attagtagta ggaaaagaat gtgaaaagac cttgaatatt ctttaattct ttttcacaaa
      961 ttccgatttc agtttcatcg gtccaaatcc ttctaccttg caatcgatat tatgatcgcc
     1021 ttctactaaa cggatgttct tgacttttgt tccttgttta atggcacctg acgcaccttt
     1081 tactttcaag tctttaatca ctgtcacgct atcgccctct aacagacggt ttccatttgc
     1141 atctaatacc actaatccgt cttctatgac agtttcttgt tcttctttgt tccattcgtt
     1201 cccgcattct gggcaaatca acaagcctcg atcttcatat gtgtactctg agccacattc
     1261 tgggcaattt ggtaatgtca tcttctttct cctttaaaat ctttagtgag cactattgta
     1321 acggaaaaag aagatcttga aaagatacat ttgtgatttt tactacaaaa ggcacgtttt
     1381 cgaacgaagt atcattctgt ttttctgctc ttatacgatt ggcatatatc ttggatctct
     1441 ttatagattt cctctacttc tgctaaatct tttgcattcg caccacttgc taatggatgg
     1501 cctcctccat gatggcgttt agccaattca ttgatgatcg gccccttaga acgtaaacgt
     1561 acgcggtaat accctgaagg ctgctgaaca aaaatccccc aagccagcac ttcgtcaatc
     1621 actccaggaa gtgaaacgat cgaagctgtc tctgaatcat cgattccata tttatcaaga
     1681 atttcttgcg gaaggattat tcttcctgca ccattttcat ccacctggat attttgataa
     1741 agataaccgg aaagtttagc gactttcatc ggcatttgtt caatctctct gttcaaagca
     1801 cttgcatcaa atggataatt tctcaatatc gaagcaatct ctaatgtacg ggacgtcgtg
     1861 gagggataca aaaagcgccc tgtatctccg acgattcctg cataaagtaa ccgtgcagcc
     1921 ttaacgttca tctttaaaga ttcaggaaac atcaaagcaa attctgcaat catctcacta
     1981 cagctacttg cttttgtatt tacccacaca agatctccat aagggtcatc attgggatga
     2041 tggtcgattt tgatcagttt ttctgccaac tgatatcttt gatcactgat tctgggtgaa
     2101 ttagctgtat cagtcacgat gactagcgct ccttggtaga agacatcttc aacttcttgc
     2161 atttctgcta aaaagcgaag cccttcaatc gtttcaccta cttgacgtat ttctttttct
     2221 gggaaacttt cccgtaatag ttcggctaat cctacttgtg atcctaatgc atcaggatca
     2281 gggcgcaaat gcctgtggat aatgatttta tcgtattttt ttattgtatc tactatttct
     2341 tgctgtactg tcatttcact cctgctttct aagaacgttc cattacttga caaacaacga
     2401 ttgctttgga aacgatggtg ttttcaataa acacttcgat atccagttta gcggaacgtc
     2461 tgccgatttc caatacacgt ggacgaatgt ctaattcgct ttccaattgg atcagccgca
     2521 aataatgtag gttcacttgt tcaatcaaga tatttcgttt ttggttcatc accattgttt
     2581 tttgtgcgac atcagaaatg atttcgctaa gtacgccaaa agaaatggtt ccaacgccat
     2641 tgaccatttg tggtgctact acgaatttga acttagggtt ttccaaacga tttccttcac
     2701 ggtcagtatc aatcgtatgg atatttccag atatctgatc cgaaatcgta tctgcaatct
     2761 gcggttgtcg ctggacaagc tgcatcgctt tcattacatc ttgccgggta atcaagcctt
     2821 ccaatgtcaa gtcatcggaa acgaccggca tgacttctag cccatcccag atcatttgat
     2881 ggctaacaga ggcaatactc attgttttct ttacactgat tggttctttc gtcatcaccc
     2941 gatcgatgat ctggtttggc gatttcccta aaacatcttt tgccgtaaca atccccatca
     3001 aacgatgatg gcggttgacg actggaaaac gcgaatgctg cgtttcttct gacaaacgct
     3061 gataatcaga aatcgtatct gtctgctgca aataatgagt cttttcagct ggcatataga
     3121 tatcactgac aagcatgata tcttttttga tcaattggtc gcttaacgct cggttgatca
     3181 tggttgcgac tgtaaatgta tcataagagg tgcgtaagat cggcatttca agatcatcag
     3241 ctagctttgc aatctcagca gtcgtattga atcctcctgt gatcaatact gcggcgccat
     3301 cttccaatgc taatttttgg acttccgccc ggttgccgac gatcattagt gatcctggcg
     3361 taatatagcg ttccatggca tgtttagtca tcgcgccgat gacgaattta ttcaagacct
     3421 tgtctaatcc tgccgccccg cctaaaacgt ccccttcaat gatcctgacg acctcgccaa
     3481 atgtcaaacg ttcaatatgt ttttttaatt tgcgttcgat acggatggtc cctactcgtt
     3541 gaattgttga aactagtccg acattttctg catctttgat tgcacggtac gctgttcctt
     3601 cactgacatt caattctttt gcgatgcttc ttacagaaat tcgattgcca acaggtaaac
     3661 tttcaatata ttctaaaatc aaatcatgtt ttgtcgccat tttctgccta cacttccagc
     3721 gtttccgaga cgctcattat ttttccttca ctatcttcca gtaactctac aaaagattcg
     3781 ggatcttgtt caatgacagg gaaagtattg taatggattg gaactacttt cttggcacga
     3841 attgtttgcg cagcctttgc tgcatcttct ggccccatcg tatagttatc gccaatcggc
     3901 aagaaagcga catcaatatc gaattcttct gcaagcaatc ctagatcact gaaataagca
     3961 gtatcccctg catgataaat cgtttttcct tctgcttgaa taataattcc gctaggctcg
     4021 cccatatact gaacgatacc gtctacttca tagcttgaac tatgctgtgc atgtacgaat
     4081 tttaccacgc cgaatggaaa cgcatgtttt ccaccgatat tcataccgaa tgaatctact
     4141 cctctgctgt tcgcataatc agcaatctca acaatactga taattgtggc ttgattttct
     4201 tttgcaatag gaatcatatc tcctatgtgg tcactatgac catgagtaat cagcaaccaa
     4261 tctgtcttta cttcttctgc agttaagtct gtcaatggat ttcctgtaat aaacgggtca
     4321 atcatcacgc gttggccatt ttccatttca attgttaaac aggaatgtcc atgatacgta
     4381 attttcatat tttacacctc gcgttttctc tgtatctttt ttattaaaaa cgatttcatc
     4441 acttgcacat caatgaaaaa atatagctgc taaatatatt ttcattggat tgatagtaca
     4501 gtatgtacaa agcaattgta acagataata caggatgcac caagtaccaa gcagatataa
     4561 tctcaagttt gaggtctctg ctctgcttag cttctcttct cgtcccttaa ctaattaaat
     4621 cagcgtaaat aggatggacg atcttttcta aatcggctac ttttatccga aggcttctcc
     4681 cgatttcacc gcttgacaca attacttctt ctttttcttt taaagaactg tctgctaaaa
     4741 aacaataatc aggatgtgcg agttttattc caataggggt gtttgctccg tggggatatc
     4801 ctgtatgcat taatacaaaa tccattccag gaaaaccaac tttacgatct ttcgtcactt
     4861 gtctcgtttt tttgtaatct aagtgatcgt tcaaaggaat caatgcgatg atcaccccag
     4921 ttttgtttcc tttcactacg agagttttac atatcaaagc tggatcaatc cctctttttt
     4981 gtagttcttc tgtgaaagat tcttctacca catgactaaa atcaagtggt tgatagctga
     5041 tcttttgttc ttctaaataa cgttcgactt ttgtttgact catcattgat tcactccctt
     5101 tattctatcg tttattataa agcgagggat gctgtattca aaaattattt gtttttcgca
     5161 aaaaaagagc aagacgagca ctctatagag aggttcatct tactcttttt gtttattttt
     5221 tatttcgacg ttgagtagct tccataatga ctggcaagat cactgggcgg cgttttgttt
     5281 gttcaaataa gtaacggcct aattgatctc ggatgtcttg ctttaatttg ctccactcaa
     5341 aatcattgct ttctaaatgt ttctcaacga ttgtttcaat catattgctg ctttctttca
     5401 tcagatcacg gctagctttt acgtaaacga atccccgtga agtaatttga ggttttgaaa
     5461 tgattttctt ttctcgccga ctgatagtga caaccgctac gaaaatcccg tcttctgaca
     5521 agatttttcg atcacgtaaa acaatgtttc cgatatcacc tatgcctaat ccatcaatca
     5581 ttacattttc tgcacttgta gttcctgcaa cactcatacg cttgttctta tattccagca
     5641 cgtctccgcg tccagtaata tagatatttt tgtatggcat ccctactgca tgagccaaat
     5701 ctgcatgagc agctaattca cgatactctc cttgaactgg cacaaaatat gttggtttca
     5761 tcaaattgat cattaattgc aagtcattag gattggcatg gccagaaacg cgcatattct
     5821 cagagatcaa cttcactgtt ccaccagctc gataaatgat atcttccgtt ttcgccacga
     5881 tggtttccat cgcgatgctt ggcgtagtag tgatataaac caaatctcct tcttcaatct
     5941 tgacattacg gtgtgtaccg tttgccattt tttgcagaga cttgatgggt tcacccattc
     6001 ggccagtttc taaaatgatc agttcttctg gatcatagtt tttcatgtct ttcaacgtaa
     6061 caagcagatc ttcacttggt aattgaagtt tttctaaacg catcgctgtg cgaataattc
     6121 gttcgaaatc ttgaccagtc aagacaactt ttcgttgtgc tttttctgct gcatccaata
     6181 cttgttgcac tcgttgcaaa ttacttgcta cacaagcgac gatgatacgc ccttcccaat
     6241 atttgatcgt atcaaatact tcatctgcta tttgggcttc agaagctaca ggctgaggat
     6301 tttctgcatt tgccgattca cttagtaagg ctaagactcc ttctttgccg atttccgcca
     6361 atcgtgcata atctgtttga tacattggga tagccgtttg gtcaaattta aaatctcccg
     6421 tataaacgat actaccttct tttgttttca gattgatccc cactgaatct ggtatcgtat
     6481 gagtggtacg gaaaaagctc actactgttt caccaaagtc tatttctgta tgctcatcta
     6541 ccacatggaa atccttaaat cctttggctg cttcattacg actgacattc aattttgcta
     6601 actcgatcgt caactctgtc ccgaatactg gtacagaaat ttttgacaac aagtaaggaa
     6661 gtgccccgat cgcatctgca tgtccatgtg ttaaaaagac accagctaca cgatcaatgt
     6721 tttcttctaa atacgtaaaa tcagggatta cgacgtcgat ccccaacaac tcattttcag
     6781 gatattttag cccacaatct aaaataaaaa tatcctcgcc cacttctgcg acatacatgt
     6841 tttttccatt ttcacgaacg ccgcctaatg ggataatttt aatggtactc acaaacttca
     6901 cctcttctta tgacatccaa gtttaaagat gtcgattttc ttctttatct aaatatatga
     6961 gccattccaa tgaagcatat gcaaaatcac tatttctcaa cttatggaga atcagcagga
     7021 aaattaccag cacatccgcg ttctgacagt tggatgggca ttttattttg gatatacttc
     7081 attaacaaaa tggatctttt gcgaaacgct atagccagaa tacttatata agtattcagc
     7141 tgttccccat tattatacaa caaataaact aaatcgctag tatcagcaat tgttagaaca
     7201 actatgcacg taaaaatccc tctgtagcat ctaggatttc ttttgctatt cctctttcat
     7261 ttcttcttcg tcggtttgaa acatactttt ttcattttac cacatttttt gcaataacta
     7321 tagtgacttt ttatatatac agaacctcat gttgatggtg tgcaaacagc ataagattta
     7381 agctagcttt aagataggtt gtctattatc aaatgcatct actaggaggg atgcaaaata
     7441 tgaattttat gaaacgtgca tggaaaagta caaaagtaaa atggggacgt tcattgttat
     7501 tgtttgccgt gttcacagcc attcttattt ttgtgctagc tggcctcacg attcgaagcg
     7561 ctgcagaaga agcagcgaat caagcacaaa aagaagttgg agcaacagtg actttaagtg
     7621 ccaatcgaga agcttcattt aaaaaacagg aacaaacttc ttcaagcggt tctaccactg
     7681 aagaacgtcc agatccaggc agtttttctc ttacaccagt ttccttaacg gacgcggaaa
     7741 aaatagctga attagataat gtaaagagtt attcctttga aagttccgcc tcagctatgg
     7801 caaaagatgg aataactgcc atcactagtt ctgattcaag tgaagacagt tcagaaacta
     7861 ctgatgcagg cggtgaacct gctggaggaa tgggcgggaa tccgcaaatg actcaagcag
     7921 actttcaaat cagtggtgtg tcagcaactg cacaaaatag taatttttct gatggaacag
     7981 caaaaatcac ggatggtgaa ggaatagatg acagcgacaa aggcacaaac aatgtagtca
     8041 ttgattctac tctagcagaa acaaatgacc tggcagtcgg agatactttc acaatcacga
     8101 gtactgaaga tgaagatacc acttatgaaa tgacgatcaa aggaatttat gaatccagcg
     8161 aaacaagcgg cagtatgggt atgaaattca acttcatgaa tccgtcgaat acactttaca
     8221 cttcttacac ttttgcaaat gaactaaatg ggacatctga cgataacacg atagattctg
     8281 ctatttacac tctatctgat ccagataaaa tggatgagtt tctcaaagaa gctgagaagc
     8341 tgatcgacac agatacattt agcttacaat caaatgattc catgtatcag tcaatgttgg
     8401 aacctttaaa caacgtggca agtttttcta aaaatgttgt tttactagtg gctgtagccg
     8461 gtattatcat acttactctg atcattatga ttaccattcg ggagcgtcgg catgagttag
     8521 gcgtcttgct atctttagga gaatccagaa gtaaaatcat tctccaattg tttaccgaag
     8581 tggctatttg tatgattctt gcattaggag ttgcttcgct tagcggaaac gtcgtcgcga
     8641 atgccgtggg acagcagcta cttgaccaac agacagaaac cacgacacaa aaccagcaag
     8701 ctcctggcgg tggacaaatg ccaggtaaca gacaagaaca aggcggtcaa cgcgatggtc
     8761 aacaaggaac cacgaataat ccatttgctg tttctgatca agtcagcgaa ttggaaatca
     8821 atgtcaaacc tgcacaattg ggactattag ccggagtagc atttggaatc agtctgtttt
     8881 ctgtcttttt agcttctata gggatattaa gattaaaccc aagaaaaatc ctattaaact
     8941 aaaggaggaa tttgatgtta cggacagaac aattgggatt ttggtatcaa gatccgcaag
     9001 atgcgcttta taaagatgta gaactgaatt tcgaagctgg aaaaatgtac gctattttag
     9061 gagcaagtgg ttctggaaaa acgacttttc tttcgcttat ttctggacta gatgcaccaa
     9121 aagaaggaaa ggtatactat caagatcgtt cactgaacaa aatcgggtta agaagttatc
     9181 gtaaaaatga tgtctcgatt attttccaag cttacaactt gcttccttat ttatcggctc
     9241 tagacaacgt catagcagca atggaaatca ctaagaccaa gcaagagaat tatcgacaag
     9301 ttgctttaga tcgactggct cgtgtaggaa ttgatgaaat attagcaaaa aaacaggtga
     9361 ccaaactttc gggaggtcag cagcagcgag tcgctatcgt tcgtgctatc tgctgtgaac
     9421 atcaactaat cgtagcggat gagcctactg gaaatctaga tgaaacaact tcacaagata
     9481 tcgtcaaact tttccaagaa atcgcccatg aacaaaatcg ctgtattatc atcgtgactc
     9541 atgagattga agtagcaaaa agctgtgatg aagtctttga actaaaaaat aaaaaatttc
     9601 aaaaaatcga agtttaaaaa acaggataat gatcaaataa aaaatccgaa ccatatctgt
     9661 ctcgcagata tggttcggat tttttatttg cttgattcaa ttagtaataa gtggttttta
     9721 ttttttttcg tcttttcgag aaaacttgtc tttgacttcg tcgcctaaat ttcgagcttt
     9781 ttctttgatg tcagcaccta gtgattgtgc tttcccttct gtttctttgc tcttgtcgtc
     9841 tgttgctttg ccgtatgctt cttttccttc accgactact ttatctttca tatctttaga
     9901 acgtcctttt aaatctgcca ttgtgcttcc tcctcttagt ttgtttattg atctaccttc
     9961 agggtaacat caacaacaga tacagacaaa cagaacgctc gttttctaaa aaagatagtt
    10021 agagctgttg attaaaaact tttttcgaaa gtcccccata ctttgacttc tttttcttgg
    10081 accattattt ctcctttagc gataactgta tcaatatcaa gcgtttcttt atccaagata
    10141 cacagatctg catcagcacc aatttcgatt tttccttttt tatccaattt caatatacgt
    10201 gctggattac tggttatagc tcgtaaggcg atttccaaag ggatactttc tttttgcaca
    10261 gcttctttga tccctactag caatgccttg gcacttccta ctcctaaccc taggaaatga
    10321 ttgttttcat caaaataagg caagcttcct tgtccatctg aggtcatcgt gaaattatct
    10381 aaagaaacat cttcctcgat caatcgtttc agtgctttgc tgaagcgaac ttcaccatct
    10441 gtcttctccc aaaaatcagg atcttcgcta gcagtgaagt caatcgtgcc accacgtttg
    10501 gcaaatgcca cgcaagcttc aaataattct ggtgtgcgat ttgcatgagt aggcaaaaat
    10561 tgtgtgatcg gaatttctgt ttcttctacg attcgtgtca atagctctag ctttcttttt
    10621 cctccgccta agtggacatt gatgatccca gcttttccgg ataacatgcc tcctacacga
    10681 gcatctgcta ctgcacgagc gaactcttca aaacttggct gagaagaacg gtgatcggat
    10741 actgcgattt ctccgattcc gataatttta tcaattgtca aaaagtcttt gatgatagaa
    10801 tctgttactg tttttaccgg caatcggtac gaaccttcgt atacataagt ggagattcct
    10861 tctgcttcca gacctttcgc tttgctgatc aacgcagtca tatctctacc ttctccgtct
    10921 gttcccaaac aaccgacaac tgtcgttaca ccagctgtag tcaattggct caatgtgact
    10981 tctggcgttc gattttgata accgccttct cctcctccac ctaaaatatg gaaatgacag
    11041 tcaatgaaac ccggggttag tacttttccc tcacctgaaa gttcttctac ttccactcct
    11101 tcgtagccgc cttctagctg ttcttctaca gccaaaatct tcccaccggc aatcagtaca
    11161 tccatttttc ctaagtaatc tggtgcaaac acttcaattt gtcgaatgat tttcatacat
    11221 ttttcctctt tctttaagta taactgatga taggatctta aaaaataatc caaacgatag
    11281 gatctttact cctttgattt taccgtaaaa tgacggttct tggagtcctt atctactgtt
    11341 tggattactt ttttatttac aggtctccag tactactgca gacctattgc aatggcaatc
    11401 atgatgaaaa tagttcctaa aacaaacagg aatccttgca tcttgatttg gaatcttgcc
    11461 cattttgccc attcgatccg tgcaacacct agcacaccaa tcaagctggc tgatacgggt
    11521 gtaaaagcgt ccataaaacc tgaaccaagc tgataagcaa gaactgctac ttgtctggat
    11581 acacctacta aatctgccag tggagccatg attggcatag tcaaagctgc ttgtcctgaa
    11641 tttgatgtaa caaccaagtt gaacaaactt tggaagatgt acatcgccca agcaccaatg
    11701 acagctggaa caccggtcaa cgccgtcccg atcccatgca aaatcgtatt caaagctgaa
    11761 ggaacagatg catcagatcc tcccaagaca agaagaatcc ctttggccat acctactacg
    11821 attgctgtac cagctaaatc agctgcacca gattggaaag aagaagcgat gtcattgact
    11881 ttcattccat ctaatttgaa aagaacagca atgattccag caactaatcc catcacaaag
    11941 aattgagaag cgatttctgg aatgtaatag cctttttgag tcacgcccca aacgatccag
    12001 acaaggacaa ctaacatctc taataagatc aatttatgcc caagcatgaa ttttttattt
    12061 tcatcagatg tctttttgat atgagaacgg aaataatcat ctgatttgta agtcaacgaa
    12121 ctttctggtt ttttacggat tttttctgca tatatcatca tataaccagc ggctaatgca
    12181 gtaacaacta cccacataat caatcggaat gtcgcaccag acaacacggg gattccagca
    12241 atcccttgag ccaccgctac gctaaacggg ctcatccaag aggtcgcatt tccgacttga
    12301 gatgcgacat aagtcacggt tactgctacg atcgagtcat atcctaacgc aatgacaaat
    12361 ggaaccatca ccattgagaa tggaattacc tcttctgcca tcccgaatgt agcgccaccg
    12421 aatgaaaaag caaagaataa tagcggtaaa gctaaacgct ccagtccctt agttttactg
    12481 ataaacgcat agatacctgc gtcgatcgct ccggtacgca taataatacc aaaagcacct
    12541 ccaacaacaa ggatcaatgc agcgatccct acagcagatc catacttatc tccagatact
    12601 agtccttcaa aaacaaaatt taagaaacca aagccgccaa aatcatctgt tccccatatt
    12661 ttggctgttt tatgcaattt tttagaagta tcatagatat tttcgccgta ctgatcgtaa
    12721 agcacatcat ctgtcaagga gatttcatct agtttttcct gcgtaagatt tttttctccg
    12781 tcagcaatga ctttttccaa tcctttttcc ggaacatcca gtttttcacg ttctgctgga
    12841 tgatcttgca gttcttctaa ttgatcaaac acatacgact tatccagctc gtaagcataa
    12901 cggaaggaat cttgacgtaa aacagtacga gtacttgttt cgccatttgc atctttgtat
    12961 tcaatatctt gcgtgctgaa ctttcctgct ggaacgacaa aagtcagtat ccatgcaaag
    13021 atgactacac caaaaataat gacatacgta tggggtgtcg ataatttagt gaagtcgcgt
    13081 ttcggttttc ctgaagaatt tgttttcttc aaatttattt cttcctttct tttttcagta
    13141 attttgatga ataaaaaatg gataatcaaa tgaattatac agtaaaaatg acatttccac
    13201 atgaataatt tattagaaaa tcactttttt ctattttgtt ttttttctcc tatgtatgat
    13261 tatttttttc tctttccatt tatattaagt attttatttc tctgtttttc cttaaaattc
    13321 tttttttatt tttagattag agaaaatctc gtcctgtttt tttcatttta caatcattga
    13381 aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat tatctatcct
    13441 tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat
    13501 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
    13561 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
    13621 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
    13681 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
    13741 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
    13801 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
    13861 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
    13921 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
    13981 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
    14041 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
    14101 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
    14161 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
    14221 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
    14281 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
    14341 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
    14401 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
    14461 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
    14521 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
    14581 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
    14641 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
    14701 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
    14761 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
    14821 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
    14881 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
    14941 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
    15001 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
    15061 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
    15121 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
    15181 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
    15241 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
    15301 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
    15361 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
    15421 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
    15481 atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat
    15541 cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa
    15601 aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc
    15661 gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac
    15721 gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca
    15781 gttaactttc tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc
    15841 caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc
    15901 ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag
    15961 tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata
    16021 tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt
    16081 ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg
    16141 ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa
    16201 cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat
    16261 ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg
    16321 cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga
    16381 cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt
    16441 tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta
    16501 cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg
    16561 gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg
    16621 ttcctagccc agcggcagga atgactgctt ttttgacctt cacttgct
//
LOCUS       DACDKJ010000066        16492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDKJ010000066 DACDKJ010000000
VERSION     DACDKJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.066"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..1452
                     /locus_tag="IV328_002306"
     CDS             1..1452
                     /locus_tag="IV328_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8938415.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="IV328_002307"
     CDS             1801..2433
                     /locus_tag="IV328_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8938416.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="IV328_002308"
     CDS             3021..4460
                     /locus_tag="IV328_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8938417.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="IV328_002309"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="IV328_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="IV328_002310"
     CDS             5222..5437
                     /locus_tag="IV328_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938418.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="IV328_002311"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="IV328_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6097..7008)
                     /locus_tag="IV328_002312"
     CDS             complement(6097..7008)
                     /locus_tag="IV328_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8938419.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="IV328_002313"
     CDS             complement(7027..8031)
                     /locus_tag="IV328_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8938420.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="IV328_002314"
     CDS             complement(8028..10151)
                     /locus_tag="IV328_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8938421.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="IV328_002315"
     CDS             complement(10156..12603)
                     /locus_tag="IV328_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8938422.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="IV328_002316"
     CDS             complement(12590..12979)
                     /locus_tag="IV328_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8938423.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="IV328_002317"
     CDS             complement(13039..13542)
                     /locus_tag="IV328_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8938424.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13779)
                     /locus_tag="IV328_002318"
     CDS             complement(13555..13779)
                     /locus_tag="IV328_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938425.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="IV328_002319"
     CDS             complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="IV328_002319"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8938426.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4913 a         3271 c         2939 g         5369 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    13741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    13801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    13861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    13921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    13981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    14041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    14101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    14161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    14221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    14281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    14341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    14401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    14461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    14521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    14581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    14641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    14701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    14761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    14821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    14881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    14941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    15001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    15061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    15121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    15181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    15241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    15301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    15361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    15421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    15481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    15541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    15601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    15661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    15721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    15781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    15841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    15901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    15961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    16021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    16081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    16141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    16201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    16261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    16321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    16381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    16441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt tt
//
LOCUS       DACDKJ010000067        16270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDKJ010000067 DACDKJ010000000
VERSION     DACDKJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.067"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            94..1368
                     /locus_tag="IV328_002320"
     CDS             94..1368
                     /locus_tag="IV328_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8938427.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1751..2479)
                     /locus_tag="IV328_002321"
     CDS             complement(1751..2479)
                     /locus_tag="IV328_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8938428.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2457..4031)
                     /locus_tag="IV328_002322"
     CDS             complement(2457..4031)
                     /locus_tag="IV328_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8938429.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            4260..5198
                     /locus_tag="IV328_002323"
     CDS             4260..5198
                     /locus_tag="IV328_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8938430.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            5210..6121
                     /locus_tag="IV328_002324"
     CDS             5210..6121
                     /locus_tag="IV328_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8938431.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            6145..7617
                     /locus_tag="IV328_002325"
     CDS             6145..7617
                     /locus_tag="IV328_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8938432.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7668..8279
                     /locus_tag="IV328_002326"
     CDS             7668..8279
                     /locus_tag="IV328_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8938433.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            8309..10222
                     /locus_tag="IV328_002327"
     CDS             8309..10222
                     /locus_tag="IV328_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP8938434.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            10350..13331
                     /locus_tag="IV328_002328"
     CDS             10350..13331
                     /locus_tag="IV328_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP8938435.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            13328..14989
                     /locus_tag="IV328_002329"
     CDS             13328..14989
                     /locus_tag="IV328_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP8938436.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            15029..15961
                     /locus_tag="IV328_002330"
     CDS             15029..15961
                     /locus_tag="IV328_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8938437.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         5280 a         2638 c         3249 g         5103 t
ORIGIN      
        1 actaacactt tttcttcgtt tcaactcaaa aagagactca cttttggtaa aatttagtcg
       61 accaaaacgt catcctacca aaggagtctc tcaatggata atcatactag aaaattactc
      121 ggattaacag ataaacattt attttttgat gaagaatggc tgattgaaaa agaatataaa
      181 ggcgttcaag cgcagtttat taaaggaaaa ttggatgact ctccttccta ttgtgaacac
      241 tgtcgatcgg ttcgtattat ccgcaacggt tcctatacga cacgcactca gatattgaaa
      301 gtcaaagaaa agttaactat tttagaatta aaacgtaccc gttttctctg ccatgattgt
      361 ggaaggacct tttctgcgaa aacggactta gttgatgaac atcatcaact aacaaaggaa
      421 ctgaagcagg caattctcat ggaactgtac gaaaatcaat cacgtaaact aatcgctaag
      481 aagtacttcg tctcggatgg gacggtgaca cggatcttgc gtgaagcaac gaagcattac
      541 cagtcacgta tgaacttcct tccgacggtg ttgtgtatgg atgagttcaa atcaatgaag
      601 tctgtttctg gttccatgag ttttatttgt gttgatggga caacccatca gctatttacg
      661 atccttgaag atcgcagact ttacaaacta actcaatact tcctgcactt tcctagaaaa
      721 gcccgtttaa aggtcaagta cctagtgatg gacatgaatg ccagctattg tcagttgctt
      781 aaaacagtct ttccaaatgc ggagatcgta actgaccgct ttcatattgt ccaacacatc
      841 aatcgcagct tcaatcaatt acgtgtccag atcatgaatc gttttcgaac ctcacactct
      901 gaaaatcaga agaagtaccg tcggctcaag cgatattgga aactcttact caaagattcc
      961 acgacatggg agccactcaa acggcattat catcgactgt ttaaacgacc aattagtcaa
     1021 acagaaatcg ttgatgagct gttaagctac aacgaagaat tgcgaactgc gtatcatttt
     1081 tgtcaattat tacgctatta tttcgttaaa cgaggtacgg aaggctttca agaaaccctt
     1141 aaaacaattt catctacgtt acctcagtcg tttaaaaaga aattcacgat cttccattgc
     1201 taccaatcgg gtatttcaaa tgcctttaag gtgtcccact caaacggcgt cactgagggg
     1261 ctgaataaca agatcaagct gattaaacga attgcgtttg gttatcgcaa cttttataac
     1321 tttcgggcac ggatctattt acaacaaggt ctgatctttg aaaactaaaa aaatatgcgg
     1381 cgcacagaac gcgccgcata tcggactaag ttttattcaa atttgtcttc accaacacga
     1441 tttgacgaag agcctattta aataactaca tccatttatt ctatcttcta aaatagcctt
     1501 tttcatttca gttaaatagt tcagcgccat ttttgcactc gacttgcata gtagtttttt
     1561 ccatattcct caacctcagc gggggttgaa gacataagga aaatcctatg cgttatgctc
     1621 aactggatct gattaaatat tctaatgtat ttttctctac catcattaaa tggttaatag
     1681 ccaaaaatca ccacttttca atggctcctt gttgtataag tataactaga agatgaacac
     1741 gtctgagaaa ttatagaagc tttttacggt aattactggg gctgtgtcca gtagtttttt
     1801 tgaagagaac agaaaaatag ctcacgtgat ctattcctac tttttgacta atttcatcaa
     1861 tagataagaa tgtgtctttc agtagagcac ttgctctttt gattcgaaag tctagcaagt
     1921 aatcatgaaa ggaataacct gtttcttttt tgaaggcccg tcctaaataa gcgggatggt
     1981 aatggaattg tgtagctaca tctttgagcg taagcttttc actataattg ttttgaacgt
     2041 aatcaatcac ttgttgaatt gactcattaa aagaatcatc agttagagag ttttgattag
     2101 ctgctgattt acgcattttt tcttcttgta ctaacttaac taaataagga aaatcaatgg
     2161 gtttcgttat gtaactttta atagataaat caatcccctt ttttgcatac tgaaaatttt
     2221 ggtgggcact aatgacaata ctagtaaagt agcgattgga tgtttgttgc aaagattcga
     2281 ttaattcgag tccagaaatt ttccccatcg taatatctgt gataagtaaa tcaaagcgct
     2341 ggttttgcag gtttttaaat gcttcgtctc cgtctgtgta gcagtgatcg ataatgatgt
     2401 cggggacttt cttcaaaaga taatgcctta tgccttctac aattgtacaa tcatcgtcaa
     2461 caattgcaat tctcaacatg catattcccc cctttttgta ttttgatagt aatcgttgtt
     2521 ccgacgtgta gttggctttc tataacaaaa cgtgtatctc caggatagag atgctttata
     2581 cgtcgaaaag aatttgagat gccgatattc tcactataag gaatatcatc attaagtttt
     2641 tgttgaatat gctccagctt gtcttggtcc attcctttcc cattttcaac gatttttaac
     2701 acataatggt tggttgtttc agttgcatag acagtcaaac gattttcctt ttttgatgaa
     2761 tcgaagccgt gtttaatata attttcaaca aatggctgta aagaaagttt tggtaacatt
     2821 aattgatcaa tatgatcaat cagctgatag tcaaagttat gtcgataacg acgttgatgt
     2881 agttgcaagg agaggtcgat attaattaat tcctctttca aatcaattat gtcgggctga
     2941 tatgtattat tgcgatacaa cttgctcaat aaatatatac tgtcgctgat ttcttcgtca
     3001 tcattcaata tggcagtcat tcgaataaat tccaaattat taaatagaaa atgaggctcg
     3061 atttgatatt gaagtgctgc atactttgct tcttgcttca accaattatt ttcataaagt
     3121 gttttttgat atgtagcatt atctaaataa aactgattga tatgcatagc gatttctttc
     3181 aaatcaccga ctttgttttc gatattgatt gacttgtagt ctcctgtaga tgaatgattt
     3241 aaggaagtaa tgatttcctg atactgctgg atatagttac gaaaacttct atttaataat
     3301 tgatgaatag caagaataaa caaactcatt aataagaaaa tcatgattgc cagacctaat
     3361 aaggtttgtg acgaataatt tggattttca tatacttgta atgtgtggtt accgatagct
     3421 atctgttgcg tttttttcca tttagaaggc gagatgtctt cagataaatt gacggaatca
     3481 ataccattag acaaagaata attgtaatgt gttggaaaga gactcttttt tagcgcgttg
     3541 atcgaaacgt ctagaatcaa tgtagcatcg ggcatgtcct cactaatgaa acttaatgaa
     3601 cgaataaaag aaattttttc gttttttgta ggtgcgtagg cacttaagta tttttcgtaa
     3661 tctaatgaga tagatatatt tttaacttca tcatcttgca caagtttttt taagttatct
     3721 actaaatagg taggggagcg atcctttgac aaaatatctt ccatgtattc agcaggtgat
     3781 gagaggtagt agtcattaag gttttcttgg aaatcataat tttgatagat ctctgtcacg
     3841 ataccggaga gaatacgatt atatgtttcc atatagtggg tgatttgctg aatgttttgc
     3901 aggttttgat ttttttcttt ttcaatcaca ttgatatagg aaaagaacaa tgtgaaacag
     3961 gcaagtaaag taataaaaaa aatcaatatg tatgtaaaac gcataataac ttttgataaa
     4021 tcgtttttca ttcaaagtcc tcattttgat aatctctaat tttatgaatc attatagcac
     4081 atattacaag tgatgtaaac gctttgcaaa cagggctact tatgattgtg aatacagttt
     4141 ccgtttaaaa actattctat tgtagaggtt taataaacta aaaataatgt ataaaaaatt
     4201 aatgtaagcg attaaccaag tgggtataat caaggaaacg ctgagagagg agagacaaaa
     4261 tgaagctaaa gaaagggtta ttaggaaatc taaaaaaaaa ccgagcattg tttattatgc
     4321 ttttaccagg tgcgttatgg ttatttatat tttgctatat accaatgttt ggagttgtaa
     4381 tcgcttttaa agatttcaga atttcatcaa ggggattctt tacaagtttg ttcaacagtc
     4441 cttgggttgg ctgggaaaat ttcaaatatc tctttgcttc gcaagatgct tggatcatta
     4501 ctcgcaatac ggttgtttat aacgttgtag gtattttgct aagtactttt tgtacagtgt
     4561 cattagcaat cattctgagc caaatggcga acaaacggtt attgaaaaag atgcagacat
     4621 ttatgatttt tcctaatttt ttatcatggg tagtcatcag ttactttgtt tacgcatttt
     4681 tgagtacgga taaaggatta attaatattt tactaagcaa ttttggtgtg gaacccataa
     4741 attggtatag tgaaccgaag tactggcctt ttattattgt ttttacgagt ttatggaaag
     4801 gcgtaggcta tggatccatc atttattacg cgtcaattat ggggatcgat actagttatt
     4861 atgaagctgc tttagttgat ggtgccagta aatggcaaca gataaggaat gtagtcttgc
     4921 cacatttaag atcgattatt gtcattatgt tgattttgtc tgttggtagt atttttcgag
     4981 ctgattttgg attgttttat caattgccaa gaaattcggg ggctctttat gatgtgacga
     5041 atgtattaga tacttatgtc taccgtggat taatgaatag cggagatatc ggaatgtcta
     5101 cggcagcggg attatatcag tcatcagttg gctttgtctt gattttgatt actaataaga
     5161 tagtcaacaa gttagatcct agttcagcac tattttagga ggaatcaaaa tgatatcaaa
     5221 attaaaacgg cagatgtact tttcgctgcc agtcgaacgg tttttcacaa tattactaat
     5281 cttatttact cttagctgta tttttccctt tattttcgta atcgtcattt cattaacaga
     5341 tgaaaaagca ttacaactca acggttacga attattgccg gctcaatgga gcactgatgc
     5401 atatcaatac ttaatacaag atggcggaca gttacttcgg tcattaggcg ttacaattat
     5461 gattacagtg attggaacgt tgattactgt ttttatgacg ggaacctatg cttatgtttt
     5521 atcaagagcc tctttccctt accggaagtt ctttactttt tatctttttt ttaccatgtt
     5581 atttgcggga gggatggttc cttcttactt agtgatgacg aagatgcttg gcttaaaaaa
     5641 tacaatttgg gctttgattc tacctcttgc ttttagccca tacaatgtca ttattttaag
     5701 gacattcttt aagaagtcga ttccagaatc gattatcgaa tccgctaaaa tggatggctg
     5761 tagtgagttt cgagtttttt tccaaatcgt tttgccttta gcgatcccag gggttgcaac
     5821 aataggttta ttttcaagct taggttattg gaatgactgg ttcaacgcat tgctgtatat
     5881 tgacaccaac aaattgattc cattgcaata tttgttaatg aagattcaaa attcaatgga
     5941 attcttagca aataataatg acattacatt agcgcagcaa caagcaattc aaaatagcct
     6001 tcctcaagaa tcaacacgaa tggcaatggt cgtagtagct actttaccga ttgcaattgt
     6061 ttatccgttc ttccaaaaat actttgttca aggattgacg atcggtggag tgaaagaata
     6121 aaaaagaata ggagatgtta aaagatgaaa aagaacatgc agaaatattt gattggtggt
     6181 gcttctgtac taacccttgg cctattaggt gcttgtggta gcagtaatgg cgaagcaaat
     6241 aatgaaacag gaaccacaaa agttacttgg tatcaggttg gagaagaacc caaagatttt
     6301 gatgaggtca tgacaaaagt caacgaaaaa ttaaaggaag aaatcaatgt agagttggat
     6361 atgcggctga ttccggacgg ggattatcag caaaagcttg gtgtgatgat caattcagga
     6421 gaagaatatg atatttgttt cgtcaatggc acagattatg tgaattatgg gaacaaaggt
     6481 gctttcatta gtctcaacga tcatttggat aaagagttga aagattatgc agctgaattg
     6541 aatgaaggat ttattgaagg tggggcaatt aatggcgaaa cttatggatt gccagtcaat
     6601 aacgatattg ccacaacgga atattggacc tttaataaag atttggtaga tcaataccaa
     6661 ttggatgtag agaatgttga aggtgtagaa acattggctc cagtattaga aaagtttgca
     6721 gaaaaaaata ctgatacgaa tattacgcca atcggtatga cccgtaccag tgtctgtatt
     6781 ccttatgaca atattatcgg tggtggtgta ccgctagcgg ttgcatttga aggcgataca
     6841 caaacagttg ttaatactta tgagactcca gaaatgatga cttatttgaa ggctatgcat
     6901 gagttttata aaaaaggata tattttgaaa gatgcagcca ctgttacgac agatccattt
     6961 cgaattgata gtaaaaactg gtttttaggt cgtaactcaa gtggttatca accattagca
     7021 caaaaaaaat tagaagctct agcgggacat ggattaattt taaaaccagc tggaaaagct
     7081 tttaaaacga ctggatttgc aagagtggca atgcatgcgg tatctgcttc aagtaaacat
     7141 ccggaagcag ccttgaaatt gttgaattat gtgaatacca atcaagaagt agcggattta
     7201 atggtttatg gaattgaagg aaaacactat gatcgcaatg aagatggttc gattacttta
     7261 acaaactctg atgattatta tcaaggcgct tgggcattgg taaatactta tctagtttca
     7321 aaaacttcac cgattacaga agaaagtaaa aaactagaag aagaaagtaa aaagtttcaa
     7381 aatgaagcac cgatgtctcc tattttaggt ttctcttttg acgcttctga ttttcgaaca
     7441 gaaattgcaa gtgtacaaaa tatcgtgtca caatatgaag tacttttaag ctcgggaaca
     7501 gttgaccctg aacaatatgt accagagttg ttagaaaaac tagaaaaagc tggattggat
     7561 aaaattcagg catctgctca agaacaatac gatgaatggc gaaaatctaa caaataattt
     7621 tttactaaat gaaaagaata gactaagcag ttagtggggg gattcaaatg aacgaaaaca
     7681 aatggaccaa aggatttaat tgggtttttc aattgttgtt actaaacttt acttatttta
     7741 tactaatttg tttaggattt ggaattttag gttttttccc tgctacgata gctttgattg
     7801 ctggcgttgt gaatcgaaaa aaatatggct tgcaagagtc tgtgaagttc atgagtaaaa
     7861 tctataagaa aaattttgtt agatataatc tatattgctg gtcagcaact ttgttgttgg
     7921 ccggcaatat tactacgatc tggctttgtt ggcagcaaag cgcaccattg ttttatttat
     7981 taagtacatt attatcggta attacactgt tcctagtctt tgcaatgttg aattgggtcg
     8041 tgatcaaaag tcttgggttg tggatgatgg gagaccgact gattgactat ttgcttttca
     8101 cagcaacgat gttgccaacg gtagtcatgc aaggcttggt gcttggaatt tatttccgaa
     8161 tattgatgat tttgcctgtg atttttctgt ttggcggaac agctgtaacg gtaatattga
     8221 ttagtaaatt aactgaaaga gcgttagcaa agttgccttg tcagatacaa tcgcactaat
     8281 tttttcacaa aaaggagtat ttctttctat gaagttaact gataaacaag tagctaatat
     8341 agcctttatc cagcaacttg agattccatt gacacaaaaa gatgaagaac aaatagactt
     8401 gatttttact gacacaaagg ctaatgtgcg acaaatcaag attactaaaa atgagcagca
     8461 acgaatttgc ttgtcttatt cacatgaaga cttgctgtat cgaggattgt ttatctgcct
     8521 tccacgtttt ttagctcaag gaggagagat gccatacatt gaagaaacct cgatcattga
     8581 gaccagttat tcattagatg cttctcgtaa tggcgtgatg aataaagaga ctctaaaaaa
     8641 attcatttct tacttagcgt tgatggggta caacacattg tatatttaca cagaagatac
     8701 ctatgaagta gaagaccttc catattttgg ttaccttcgc ggagcgtata caaaagaaga
     8761 agtgaatgaa attgttgaat ttgcttctgg ttttggcata gaagtagttc catcgatcca
     8821 gacccttgct cacttgaccc aatttttgaa gtggtatgct caacaagaac tgatggatga
     8881 tcaaaatact ctactgatcg gtgaagaaaa ggtttatgcg gcaatcgaaa agatgattat
     8941 tgcctgcaaa gaaatgtatc agacagagcg tattcatttg ggaatggatg aagcctatca
     9001 agcaggtctg ggacgatatt taagcctgta tgggtattgt gatcggacaa ctttaatgat
     9061 tcaacatatg gaccgtgtgt tatcaattgt tgataaatat aagatgaaac caatgatttg
     9121 gagtgatttc atttataagc tattagatga aaccggtaaa gataggtatt acaatcctga
     9181 ttttaaactc aaagcattag cagctaaagg attaccagca ggattaacct atgttcattg
     9241 ggactatggt tcagatgaag tttcctccta tgagaaagta attgataacc atttgaaatt
     9301 ttgtgacatt gatcattacg ttttagctag tggtgcccat atctggggga gactagcccc
     9361 gaatcatggg aaaacagaag caattgtttc tgcagctgta aaagcttcta aaaataaagg
     9421 tgtacgttct gtgatgttaa ctacttgggg agatgaaggg caggaaacag agcattggca
     9481 ttccttaatt ggggcattaa aattagcaga ttatgtctat tctgaagaag agtggaatga
     9541 ttttgaggaa atctacgatc gtttattagg aacaggtagt tatcaattaa tgtccgaatt
     9601 acgatatttc gatgaagtgg ataccattac aaataacaac ttacaaatgg cttccgtgag
     9661 taaactgcta ctttggcaag atccgttagt tggtatatat gactttcatg tagccgctca
     9721 taacaaaatt gccactagaa caattggtac ttattatcat gagttagctc agaaattaga
     9781 tatgaataaa aattggaatc caaaacaacc aattttaaca actattcgtc aacgatatat
     9841 acttttagct gaaattttag cacaaaaggc agaactaggc atctatttac aacactgctg
     9901 tctggtagaa gaccttgcag gattaatgga attagcgaat cggtgtgaag cactagccaa
     9961 tcgagtaaat gaattggcat ctattcatga aataatttgg tatcaaacat acaaaaccta
    10021 tggttgggaa gtattagaac aacgatatgc tgcattagct catcgtctga aaaccgttgc
    10081 gcgtcgtatt aaaaatcatc ttcaggggca agatgcccta gaggagcttt tggaagaacg
    10141 attaccgttt tgtcaatatc aagtgccgct tgaaatttct ggctttaatt atcggcaaag
    10201 ctcggtgtca ggctacaatt aacgaaggaa tcttcccgtt gaacggacca cttgctcatc
    10261 taatgggaaa gtggttcgtt ttctattcat aagtagctta ccactaactc ttttattaag
    10321 aagagaaaaa aagtaggaag tgagaaaata tgttatcgga ctattatcaa aatctgtcag
    10381 ttataagtgt caatacagaa ccattacgta attattatat tccttatgca agtaaagaag
    10441 aagcaaaaca agagttagaa agggaaaaaa cgagccagtt tatcgatctc aatggtacat
    10501 gggattttca gtattatcct tctgtccaac agttaccgga aaattttttt caagaggcct
    10561 ttactgaaag ctatgataga atttctgttc ctggagtttg gcaaacagag ggatttgact
    10621 tacctcagta tatcaatgta tcttatccaa ttccttttga tccgccttat gtacctaatg
    10681 ataccccttg tggtgtatat cggcgtcagt ttcaaattga gcaaacagat gcacgctttt
    10741 acttaaatct agaaggagca gatagtaatt gttttgtttg gataaataga aactttgtag
    10801 gctatgacca agtgagtcat agtacatctg aatttgatgt gaccaagtat ttacaggttg
    10861 gggttaatga aattcaaatt ctagtgatga agtggtgtga tggtacctac tttgaagatc
    10921 aggataagtg gcgaatgagc ggtctatttc gtccagtgta tcttcttcaa cgaccaacta
    10981 tgcatctgac aagttatcaa ctacggacgt tgactgatcc taaaaaagga agcggaacat
    11041 taatggtttc ttttcaacga tttactccta ttgaagtatc gttacctatt tcttacgaat
    11101 tattccatcc tgatggacgt ctcatagcga aaggggagtg ttactcggaa caacaaataa
    11161 atttggatgt tgaagattgc cttttttgga cagcagaaac gcctaaatta tatacattac
    11221 ttttaacatg taatggtgaa tattttaaag aaaaagttgg ctttcgagca ctgacgatca
    11281 agaatcgtaa gttattgctg aacggaacac ctattaaatt atatggagtc aactatcatg
    11341 acgtgaatca gcaaggcacg ccagtgatga cccgagagca gttacgtgca gatttgttat
    11401 taatgaaaaa attaaatgtg aatactgtac gaacggcaca ttaccctaaa tcaccagatt
    11461 tttatgaatt ggcggatgag attggtctgt atgtaatcaa tgaagcagat ttggagtgtc
    11521 atggtgtggt taatctatat gatactgaag cgaattatca actaattgct aatgatccga
    11581 cattcgaaaa gccgattatg gaccgagtga tccgttctat tcttccttat caaaatcgaa
    11641 gctctatcat tatgtggtct atggggaatg aatctggtta tggagtaaat tttgaaaaag
    11701 ctcaagcttt agcacggaaa ttggatgatt cacggttgat tcataatgaa cgctcgattg
    11761 aaccagttat tgaccaagtg aatgatttta gtaacttgga tgttattagt caaatgtatc
    11821 ccccactgga agatgttcat cgtcattgtc aaagaagtga tactgaaaaa ccttttttta
    11881 tgtgtgaata tagccacgct atgggcaacg gtcctggaga tcttaaaacg tacttcgatt
    11941 tgattcaaca gtatgattcc tttcttggcg gttgtgtctg ggagtggact gatcatgcaa
    12001 tcaatagagg aactttagaa acgccatctt atttatacgg tggtgatttt ggtgaaaaat
    12061 atcacgacgg caatttttgc gtagatggtt tattgacccc agatcggcaa ttaacagtag
    12121 gtgcattgga gtaccgtaat attcattcgc ctattcaact gattgctgta gatgaggagc
    12181 tgcaagaagt aaccttgagc aatataactg cttttacagc cggttcagca tttaaccttt
    12241 ctcttgaatg gtatatagat ggaaaaaaaa cagtttatga agtaatgaat cctttcatac
    12301 ttgagcctgg caaaagttgt tcattgccca tcacagtacc tacaacgatt catggtgctc
    12361 atacggttgc tgtttcgcta tctgtttata aaaatgatga gctttatgga cagcaacagc
    12421 tctttcttaa aaaagaaaaa atgagattgc caagaactgc tcatcataat gatgcaccac
    12481 aaatttcagt aaaaggcaca gagctgataa tcaaaggaga atcctttatt tatatatatc
    12541 aattagcaaa agctgcgttc acttctgtaa agatcaatca aaaggaactt ttggaacgtc
    12601 ctactcaatt ttcaatttgg cgtgcaccaa cggataatga tcgtaactta gtaaacaaat
    12661 ggaaagcagc tggatatgct gaaatgtctt tagaaatcag aaaaaaagac tggaaattcg
    12721 tagatgatca tctcctgatc gaaacgtctt atcgaggagt tattccagga ttacagccaa
    12781 tttttacaat gcacatacag ttgaaaataa atggtgcagg cgtaatcaac caaaaagtat
    12841 cagtggagaa aaatccgatt tttccagatt tccctcgctt tgggatggac tggccgttga
    12901 atgatacttt ttcgaaggtt tcttattgtg gctatgggcc gtatgaaagt tatcctgata
    12961 agcactatgc tgcgatgttt ggtatttatg agatggagat tgaggaattt gatcagccat
    13021 atatcaaacc acaagaatat ggtaaccgct ggcaatgtga ttgggtatca atcaaaaatc
    13081 atcaaggatc taccatgacg gtttatggaa actctaattt ttctgctctg ccttatagtc
    13141 aagctcaatt aaccgaaaag tcacaccatt atgaattaat aaaagaaaat agaacttggt
    13201 tcaatttaag ttataaacaa aatggtatcg gctcacacag ttgcggtcct tatttagaag
    13261 atcgtttccg ttttatggaa gatacttttg actggaatat agtgattact ttcaaaggag
    13321 aaaatagatg aaagaaacaa agatcaatca agtcaaaaaa aaattatctc gttatcaaga
    13381 agttgtttat cgaaaccatt gcttaaaatg gtcaaaaaat aagctgaatg ttcgttttaa
    13441 tggattattt tatcgtttcg aacagttaga agaagtctgg aaaccggtaa cgaatttcca
    13501 atcatgtcct gtacagctca taaatgatcc aaaagaaaca tacgatttat gtataacaca
    13561 cctttataac cagcatttga aaaatgacgg attttattta aaggttacta atgacaatca
    13621 acttacgatt gaatcagcaa accttcgagg gtttcaatat gctatggaag ccttattaga
    13681 gagttttttt tcggtaggag atcaattatt attaccggaa ttgattctga aacatgaacc
    13741 atcagtgaaa attcgaggaa tcattgaagg gttttatggt atcccctgga cacatgaaat
    13801 gcgcctctct cttttttcat atatgaaaga taaccaattg aatactttta tgtatgcccc
    13861 taaagatgat gaattattac gtaaaaaatg gcgtgaactc tacgatgcgc aagaattaga
    13921 caaatttaag gagcttttat ctgctgctga ggctagcaat atcgattttt ggtacttaat
    13981 tagtcctgga aatgatattg atattacgtg tgaagaagac attcaagttc ttctgaaaaa
    14041 gttagaacaa ctgattgagc ttggcgtatt tcaatttggt ctattaatgg atgatattga
    14101 ctatcaatta aaaggtgctg ctaaacgccg ttttcgagag ccagcattcg ctcatgccta
    14161 tttagtgaat cgagtagagg aatatcttag cactcggttg tccaatcatg aattagtcat
    14221 ttgtcctaca gaatatgata atcgtcatgg ttctcgctat ttagcaacgt taagtcaaga
    14281 aataccagaa caattaccgt tcttttggac tggcccaagc acgttagcgg cgtcaatttc
    14341 tacagaagag ctgcaggaaa tggcttctgt ctatcaacgg ccgatgctga tttgggataa
    14401 tattccggta aatgattatt tagaggataa agaactattg ttcatgagtc catatgagaa
    14461 tcgaacaccg aatctttcta aggagagata ccaggttaca ggagtagttt cgaatccaat
    14521 ggctcaatta gaagcttcaa agtttacgat caatagtatg gctaattact tatggaactg
    14581 tgagcgtttt gatcctttgg aaacttggac gtcagtcgtt gaaaaagtag ttggaagtaa
    14641 actacaacca gcttatctta ccttaaccaa tgcttttcca aatcattaca caagcagtct
    14701 gctttcagat gaagaattac tgctagccaa agacccagaa tgggtcacaa aagaaatggc
    14761 tgctttagtc caagcagtaa aatggatcca gcaagaacaa catacatcta gattgagaac
    14821 agaattggag ccttggttac aagcaatcac agaaagttgg actttctggc aagaatggca
    14881 attaaaaaaa gatccaaaag aagcagaaga atggttggct aagaaaaaaa cacaccgcat
    14941 aggacgtgat ttagtaactg cccatttaga gcaaattatc aaaagttaga tatcttaaaa
    15001 aataaaaagc aagaaaaagg agaacaaaat ggtcaagaga ttaattagtg caaactacag
    15061 tgatgtcaag aaaatgacgt caaaagagtt gaagcaatcc atcaaagcga gtgaaggacg
    15121 ggtgattgtt tgtgaaacgg ttgctaatac tcttccacaa tctggagaaa ttacgaatgc
    15181 agaagttgcg tcagcatttg gtgcagattt gattctttta aatggtttcg attgttatcg
    15241 tcctgcagtt tttggactac caaatgtgac gattgagaag gtttttgatc tacagcaaac
    15301 gattgaaaac ccgattccta aattaaaaga actagtcggt cgtcctgtgg gaattaattt
    15361 agaacccatt gcccctaaaa caaatatgat gagtgagaaa gtagaaatca gccaaggaag
    15421 agcaagctct gttgaaacga tacaaaaagc agaagaacta ggagtcgatt ttatttgctt
    15481 taccggtaat ccaggcactg gagtaaccaa tttagaaatt actaaatccg taaaaatggc
    15541 aaaaaagtat ttttcgggat tgattattgc tgggaaaatg catagcgccg gctcagaaga
    15601 gccagttgtg aataaagagg cgattatttc ttatgcggaa gcaggcgcag atattttact
    15661 agttccttca gtagggtcta tcccaggctt tttagagcaa gacctaattg aagcggttcg
    15721 gttggcaaaa aaatatgatt tgttaacgat gtctgcaatc ggtactagtc aggaaagtgc
    15781 ccgaccagaa acattgcggc aattagccat tatgaataaa agttgtggtg ttgatttaca
    15841 acatattgga gatgctggat acgggggatt ggcaccagca gaaaatattt tcgaaacctc
    15901 ggttgcaatt agaggaatgc gacataccat caactgcgtt agccgctcga tcaatcgcta
    15961 acaaaattaa ataaaaaatg gttaaattga ttatttaagt gggaacatac ttgagatatc
    16021 agctaacttc tgtcacttta aaaaagggac tgtgacagaa gtgaaaatag acaaaaaata
    16081 tccgaacaag agactaggat agcagaaaaa ctatcgtttt gcaacgagga ccaactggac
    16141 cgtaggagca attctcttat tgttcggata tttcgcttgt tcaaggactt atgtctgtag
    16201 tgcccttttt tggtaggaaa aatggtaggg aaccatatca tttttaatga aaatcaagca
    16261 tttcaaaagt
//
LOCUS       DACDKJ010000068        16225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDKJ010000068 DACDKJ010000000
VERSION     DACDKJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.068"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IV328_002331"
     CDS             138..1475
                     /locus_tag="IV328_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8938438.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IV328_002332"
     CDS             1523..3652
                     /locus_tag="IV328_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8938439.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IV328_002333"
     CDS             3627..4316
                     /locus_tag="IV328_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938440.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IV328_002334"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IV328_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="IV328_002335"
     CDS             complement(5546..5740)
                     /locus_tag="IV328_002335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938441.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="IV328_002336"
     CDS             complement(5790..6230)
                     /locus_tag="IV328_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8938442.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IV328_002337"
     CDS             complement(6234..7079)
                     /locus_tag="IV328_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8938443.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IV328_002338"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IV328_002338"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8938444.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IV328_002339"
     CDS             complement(8703..9650)
                     /locus_tag="IV328_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8938445.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IV328_002340"
     CDS             complement(9873..10223)
                     /locus_tag="IV328_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8938446.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IV328_002341"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IV328_002341"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8938447.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IV328_002342"
     CDS             11646..11966
                     /locus_tag="IV328_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8938448.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IV328_002343"
     CDS             11988..12452
                     /locus_tag="IV328_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8938449.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IV328_002344"
     CDS             12657..13262
                     /locus_tag="IV328_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8938450.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IV328_002345"
     CDS             complement(13301..14590)
                     /locus_tag="IV328_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8938451.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="IV328_002346"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="IV328_002346"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8938452.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16225
                     /locus_tag="IV328_002347"
                     /pseudo
     CDS             15700..16225
                     /locus_tag="IV328_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5134 a         2971 c         3297 g         4823 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttga
//
LOCUS       DACDKJ010000069        15263 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDKJ010000069 DACDKJ010000000
VERSION     DACDKJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.069"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..220)
                     /locus_tag="IV328_002348"
     CDS             complement(<1..220)
                     /locus_tag="IV328_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938453.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKN"
     gene            complement(656..1222)
                     /locus_tag="IV328_002349"
     CDS             complement(656..1222)
                     /locus_tag="IV328_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8938454.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1222..2178)
                     /locus_tag="IV328_002350"
     CDS             complement(1222..2178)
                     /locus_tag="IV328_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8938455.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2178..2834)
                     /locus_tag="IV328_002351"
     CDS             complement(2178..2834)
                     /locus_tag="IV328_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8938456.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3204..5222
                     /locus_tag="IV328_002352"
     CDS             3204..5222
                     /locus_tag="IV328_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8938457.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5649..8063
                     /locus_tag="IV328_002353"
     CDS             5649..8063
                     /locus_tag="IV328_002353"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8938458.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8411..9964
                     /locus_tag="IV328_002354"
     CDS             8411..9964
                     /locus_tag="IV328_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8938459.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10345..12129)
                     /locus_tag="IV328_002355"
     CDS             complement(10345..12129)
                     /locus_tag="IV328_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938460.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12144..13865)
                     /locus_tag="IV328_002356"
     CDS             complement(12144..13865)
                     /locus_tag="IV328_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938461.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13965..14726
                     /locus_tag="IV328_002357"
     CDS             13965..14726
                     /locus_tag="IV328_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8938462.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4966 a         2790 c         2995 g         4512 t
ORIGIN      
        1 tatttttaaa cttagtctct acctctaata tatatcgagt ttttattttg ccttcagaga
       61 atactgtttc tttttttgca gttacaggtt gtttattttc ggaaaaagct aatatagcta
      121 aaattgaaac atccatctgg aatttatctt ttttactgct ttgatcataa aagtctgcat
      181 attcatcact gatatttttt ctaataaatt cttccatcat aaaaatcacc tcaaaacgat
      241 tatacgaact tacgttcttg ttgtaaagcg atatttgagg ggcaaaaaag gggcataatt
      301 tgactgatat taagttagct attgatatat gaatgacata caaaaatata aaatctttta
      361 aaaatgctta tatatcaatg tttttgccat tcttttatat atctgttata tgtcaaaaaa
      421 atgtgttcaa tttcaatttg tatttgaata aagaggttgt gaaaaagctg tcctgcatca
      481 agtaataaga acagccaatc aaaaacagct tctcatgttt ttgatttggt tggacttatt
      541 accgcagata taagcttttg agcaccgtga ataaagaggt tatgaaatca acgctctaac
      601 ctgagtatta gagaaaaaaa cgcaaaacat atcatttatg ttttgcgttt tttatttatc
      661 tttttttctc aatacaatat aaaataggcg gctggttctt ttggttgata aaccgataat
      721 tcagcacatt atatgtttcc tgtggcagtt tggaacaaaa agaatcaacc atatccagtt
      781 ctttttctcc accttcatga ccatagtaga cgacaaggat caatcgtccg cgaggcacaa
      841 gtcgtttcaa tatttcttcc atagctgtgc gtgtagtctc tggcatagtg atgattgctt
      901 tatcactttt tgggagatac cctagattga aaatccctgc tttgagattt tgttgttcag
      961 ctatcacttc tcctaacgtt tcgtggcctt gtagaaacaa tgagacacgt tcttctaact
     1021 gacgctgttc tagcctttca cgagtagcat cgattgcttg ttgctggata tcaaaactat
     1081 agacatgacc agtttttccg atgtgttctg ctagaaaaac cgtatcgtgg ccattgccca
     1141 tcgttgcatc tacgacgtga tcccctggtt gcaagatttc ttgcagtaat gtatggctga
     1201 aatgcaaagc agtttgcaac atcagacgct cacctttttc cgtacatcat attttccttg
     1261 gaagctatct ctgcgaagaa gttcttcatc aattgcattc aagacttccc attttttcaa
     1321 gctccacatt ggtccaatca aggaatccca cggcgcatcc cctgtcaaac gatggatgat
     1381 gatctctttt ggtatcattt ccagctggtc gcaaatgaca ttgacatagt caggacggct
     1441 cattaattga aggcgacctt catgatagtc ccgcaacatt ctcgttttgc gcatcagatg
     1501 catcaaatgg agcttgattc cttggatatc tgaatctaaa atggtgcgtc tgacattttc
     1561 tttcatcatt tctaaacttt cgcctggtaa tccgttgatc aaatgcgtac aaacacggat
     1621 attatgtttg cgtaatttag ccacgccatc taaataagtt tgataatcat gtgctcggtt
     1681 gatcagtttt gaagtttctt caaaagttgt ttgcaatcca agttctaccc ataaatacat
     1741 ccgctcgttc agttcagcaa ggtaatcaac tacttcatct ggcagacaat ccggtcgtgt
     1801 accgactgat aatcctacga cacctggtaa attgactact tgctcaaatc gttctttgat
     1861 tatttcaact ggcgcatggg tgtttgtaaa attttggaaa taaacgatgt attgttggac
     1921 atgaggccat ttttgatgca tcatgtcgat ttctttctgg aattggattg gaagagggtc
     1981 ttcaggtgca acgatcatgt ctcccgaacc agatacactg caaaaagtac aaccgccatg
     2041 ggcaacagtc ccatctcgat tcggacagtc aaatcctcca tcgatcggca ccttgaaaat
     2101 tttcccgcca aactcatttc gtaaagcgta attccaagaa tgatatcttt tatttccttc
     2161 tgaataagga aaagtcatta aaagtccttc tttctgtaag agttatctat ttgttttctt
     2221 ttttctctat acactggata agtcaggcac agccaagaaa gactgagtaa gtaaccgccg
     2281 atgatatcag taggaaaatg aacgcccaga taaatgcggc ttatcccgac acttactata
     2341 ataaacatca tcagcaattg aagcaatgtg cgtaaggttc ttttttgaac aaaagctgga
     2401 agcagaaata gtaaagaacc gtaaagaatc atactgcctg ctgcatgtcc actagggaaa
     2461 ctgtaacttg tttcaactac taagtgcgcc aatgaaggac gctctcgcat gaaaaatagt
     2521 ttgatcaccg ggttgatgat tccagcaatt acaataaatc cagaaacgaa ccaaaatgtt
     2581 tctgcccttt ttttcttata ccacatccat aaagccgcac aagcaaataa aatgatgacg
     2641 gtaagaggat taccgaattt agtcataaag agttggtagc tgttcagatc aggataagaa
     2701 gctctgacaa tgcctgtaaa ccactgatcg aaaccagaaa gccacgacag atggaacttt
     2761 acgatccagc ccaaaatgaa aaacaggaca agaaagaagc taccagccac ctgaaatgat
     2821 gttttatatt tcatacaatt cctcctttga agcaatgact tattatagca tttttggtcc
     2881 agcgaaaaaa acggtaaaga gataaaacca atgtatgatt cctctttgaa taaccaatat
     2941 tcttataaaa aaaataactg taatcggaaa gacatttttt ggtaatgcaa atgaaatgtt
     3001 agggaaataa aatcagtgtt tttcgaaatc cttttaaaca agaacgtaga cgtaggaaga
     3061 acttgaaaat gaacagaagt tgttcattac ggtaaattac aatatgatta caaaaaacaa
     3121 caaagctctt aaataacttt taataaatat taagagaagt tataattatt aataatagat
     3181 aaattgaagt tggaggatag aagatggaaa acattgcaag aaaagaacgg agacgtttag
     3241 aacagaccaa acgttttcga aaagcaaaac gaggggcagc aatcgtggga actgcgatgg
     3301 taggctgttc tgtcgctgcc ccattagtac agccggttcc tgttcaagcg gatgaaacac
     3361 ctgttcagtt cggcgcaaga atcaatacag cggcatttat cacagaaatt gctacttatg
     3421 ctcaaccaat cgctcaagcc aacgatctat atgcatcagt gatgatcgca caagcagttg
     3481 tagaaagcgg ttggggcagt agtgcgttat ctcaagcacc ttattataat ctttttggga
     3541 taaaaggcag ctatcaagga caaaccgtct acatggatac gcttgaattt ttaaataatc
     3601 aatgggtaaa taaaaaggag cctttccgac aatatccttc ttttgctgaa tctttcaatg
     3661 acaatgccta tgtacttaga aatacttcat ttggcaacgg ttattactat gccggtactt
     3721 ggaagagcaa cacgaaatcc tatacagatg cgacagcttg tttgacaggg cgttatgcga
     3781 ctgatcctgg ttacgcaggc aaattaaata atatcatcgc aacttacgga ttgacccaat
     3841 atgatacacc agctagcgga aacgcaggcg gtggagcgac tacaggtggc aataatactg
     3901 gaagtgcagg tagtactggc ggttcaagca attctggtaa tacaaactca ggttcagcaa
     3961 cttcgaccac ttatactgtc caatctggtg attctgtttg gggaatctca aataaattcg
     4021 gtatcacaat ggatcaattg atccaatgga acaacatcca aaacaatttc atttatccag
     4081 gacaaaaact gacaatcaaa ggtagccagg aaaatggctc ttcgacgaat aattccggta
     4141 acaataccaa ttcttcagga aatgctggta catcaaacgg aggacaaaca actggtgcga
     4201 aatacactgt tcaatctggt gattctgttt ggaagatctc aaatgatcac ggaattacga
     4261 tggctcaatt gattgaatgg aacaatatca agaacaattt cgtttatcca ggacaacagc
     4321 taatcgtatc aaatggcggt tcttctaatg ctggagcaaa tactggctca aatactggga
     4381 atacaagtgg ctctagttcg tccagctcaa gttcttctaa tacagcttca ggaacgaaat
     4441 atacagttaa agccggagac tctgtttgga gcgtctcaaa taaatacgga attacgatgg
     4501 ctcagctaat ccaatggaac aatatccaaa ataactttat ctacccagga caacaactga
     4561 ctgtttcaaa cagtggatca acaaacagca cgtcaggtaa tacaggttcg tcaaccaata
     4621 caaataacag cagttcagtt aatactggca gcagttcgtc tcaaggcact tataccgtga
     4681 aagcaggtga atctgtttgg agcgtggcaa ataaaaacgg catcacgatg gatcagttaa
     4741 tcgaatggaa taatatcaaa aacaacttca tttatccagg acagcaattg atcgttaaag
     4801 gtggaacttc ggcaaataca aatacaggtt caactacttc tgcagcgaaa ccaaatacac
     4861 cgaatacgtc tgctacgaca agtacatcat ccactggtaa tacgatgtat accgtgaaag
     4921 cgggcgagtc agtttggagc gtagcgaaca aacatcatat cacgatggat cagttaatcg
     4981 aatggaataa tatcaaaaat aatttcatct atccaggaca gcaagtgatt gtgaagaaag
     5041 gttcatctca atcaacaaac caacaatcag ttactggttc gaaaacctat actgtgaaat
     5101 ctggagaatc tgtttggagt gttgctgaca gtcatggaat tacaatggct caactaatcg
     5161 aatggaacaa tattaagaac aactttattt atccaggtca aacgttgatt gtagctaaat
     5221 aaaatagctg ttcgaagaaa taacgattga attccacaaa aatatttgtt actataaggc
     5281 tacttaaata tgacaacact tgaaaaggaa gtagtaaaaa gactgaagtt tcagagagcg
     5341 catggctggt gagaatgtgc acggatgctt ttgaactcgc ctttgagttg cccttgctga
     5401 atacccgagt aagcaagcgg cggtgacggc cgttacaacg ttcgaggcct tttcactaag
     5461 gtaaaattag gtggtaccgc gtagattacg tcctataaaa agagcaatct ttttatagga
     5521 tttttttatt tcttttgtga agtttgagaa aacaaagcaa aaggaaataa aaataaacag
     5581 attctgccgg ttttttcgtt atatttataa tagaaacaag tgttttcaat agaacaggag
     5641 gacagcaaat gagttacaat cataaagaaa ttgagaaaaa atggcaaaaa tactgggcaa
     5701 aaacgaatac tttcaacacc catgatgatc ctgaaaagcc aaaattttac gcactggata
     5761 tgtttcccta tccatccggt caagggctac atgtagggca tcctgaaggt tatacggcaa
     5821 cggatatcct ttcacgtgtg aaaagaagtc aaggatacaa cgtgctgcat ccaatggggt
     5881 gggatgcatt cggtctgcct gctgaacaat atgcgctaga tacaggaaac gatccagctg
     5941 agtttaccca aaaaaatatc gaaacattcc gacgccaaat caattcttta ggatttagtt
     6001 atgactggaa tcgcgaagtg aatacaactg atccggagta ttataaatgg acacaatgga
     6061 tctttacgaa gctttatgaa aaaggtctag cttatgaagc agaagtagcg gtaaactggg
     6121 tacctgaatt aggaacagtt atttcaaatg aagaagtgat cgatgggaaa agtgaacgtg
     6181 gtggctatga tgtagtcaga aaaccaatgc gccaatggat gctgaaaatc actgcttatg
     6241 ctgatcgctt attggatgat ctagaattag ttgattggcc agaaagcatt aaagaaatgc
     6301 aaagaaattg gatcggtcgt tcagtaggag caaatattga atttaaagtg gctggtacgg
     6361 ataaatccta taccgtattt acgacacgac cagatactct attcggtgca acttactcgg
     6421 ttcttgcacc tgaactagat cttgttagag aaattacgac ccctgaacaa aaagctgctg
     6481 tagaagctta tatcgaagag acagctaaaa aatcagatct aaagagaacg gatcttgcaa
     6541 aagaaaaaac aggagtattt actggtgctt atgcaatcaa tccagttaat ggcaaggaaa
     6601 tcccaatctg gatcgccgat tatgtattat cttcatacgg cacaggagcc atcatggctg
     6661 ttccagcaca tgatgaacgt gactttgaat tcgctcaaac tttcggacta gaaattcttc
     6721 cagttatcga aggtggagat gttgaaaaag ctgcttacac agaagatggt acgcacatca
     6781 actctgaatt tctagacggc atgaacaaac aagaagcaat cgataaaatg aatgaatggc
     6841 tagaagaaaa tggcgtaggg aaaaaagaag tcagctatcg cttacgtgac tggctattct
     6901 cccgtcaacg ttactggggt gaaccaatcc caatcatcca ttgggaggac ggaacagtca
     6961 cagctgtccc agaagaagag ctaccattac ggttgcctaa gacaaaaaac atcaagccaa
     7021 gcggtactgg agaatctcca ttagcaaata tcgaggagtg ggtcaatgtc gttgatcctg
     7081 taacaggtaa aaaaggccgt cgcgaaacga atacaatgcc acaatgggca ggaagctcat
     7141 ggtattacct gcgttatatc gaccctcata acaaaaaaga actagcagat tatgaaaagt
     7201 tgaagcgttg gctaccagtt gacatctaca ttggtggagc agaacatgcg gtacttcatt
     7261 tactatatgc acgattctgg cataaattct tatacgatat cggtgttgtt cctacaaaag
     7321 aaccattcca aaaactatac aatcaaggaa tgatcttagg cgaaaacaac gaaaaaatgt
     7381 caaaatctcg tggtaatgta gtcaatcctg atgatgtggt cgaaacttac ggtgcagata
     7441 cactgcgcat gtacgaaatg ttcatggggc cattagatgc ttcgattgct tggagcgaaa
     7501 atggcttaga aggaagccgt aaattcttgg atcgtgtatg gcgtttgatc gttgatgaga
     7561 acaataaaat gcgtgaccgt atcactacat tgaatgatgg caagcttgat aaagtgtatc
     7621 atcaaacagt gaaaaaagtg acagaagact atgaaaatct tcatttcaat acagcaatct
     7681 cccaattgat ggtctttatc aatgaagcct ataaagtgga cgctctgcct tatgaatata
     7741 tcgaaggatt tgttcaacta ctcgcaccga ttgctcctca catgggtgaa gaactatggg
     7801 caatccttgg aaatgatggt ggtatttctt acgcaccatg gccaacatac gatgaagcag
     7861 cacttgttga agacgaagta gaagttgttt tccaagtaaa cggtaaagtt cgtgcgaaaa
     7921 gcaatgttcc ccgagactta ggaaaagatg agctagaaaa agcagcatta gccaatgaaa
     7981 tagtgcaaga atatattgaa ggcaaaacag tcagaaaagt tattgcggta ccaaacaagt
     8041 tagtcaatat cgttgctaat taattggctc tttgtcaata aggactgatg agttgtgcaa
     8101 aatcaaatct gggccagaat gaacttcatt ctgtctcaga ttttttgtta tttcaaattg
     8161 attaattgat tgatcaaaaa ggttttgatt ttcatgttct caaacacgtt caatgtcttt
     8221 ttcttctgaa tggtcttgta agagatctct gtaatcatca aatttttgtc tattaatctt
     8281 agtatttttt gatgtataaa ctacctgaaa ttgagagtac ataccaaatt cataaatttt
     8341 ttattagtgg gactagtgat tgtatggatt ttgacgcata atgaatgaag tatgagaaaa
     8401 ggagagacaa atggatcaag aactatttat tatcgaagta ttatcagcta aacaaaaaat
     8461 tatcgagaaa gcgaaaaaaa gagcgaaaag aataatgttc aagcttttaa gcttagtatt
     8521 tttctctggt ttgctttttt tcggagctgt atcgttccta tatcgccata tgagtgcagt
     8581 agtccgttca gctgaaggga tgcaaaaatt aaataatttg tatcactttt cgtggggagt
     8641 tggcggaata acaattgttg tgcttattgt ttttcttcta cttatttggc tccgttacca
     8701 tcaattgtcg cgtcatttcg atcaatggct tagtcaaaga gcacaacagt ttttacataa
     8761 taaaccaact tctcaagatg atgactatct ttattttgag aagacttcga cgagtcaagc
     8821 ttttcggata caaaaaagtg attgccagtt attgctgaga tcacttgatg atttacctat
     8881 ctacttaggc gaaagcaaat caaaattcgg aatgaacaca ttccatattt ttttactaga
     8941 aaacgaaccc agatgggaaa aaagccagtc ttcgcctggt ggatacctga caactaagat
     9001 gagtacatta gtcactggtt ttttcctcat tcttttagct ggaacaatct attatgggag
     9061 atacactcag ccaaaaatgt cttctcatat aaatgctgct tctcacatga caatgaactc
     9121 aactaacgat acgcatatac aaaagacaaa tgaagggttg ttagctgatc ctaagcaaaa
     9181 ggtaaatttg acaacacaaa agataaatga tctcaaactt gatacagaga cgcactctct
     9241 ctatatgaca acagatagtg gtgctaattg ggtatttgtg ccgattgatt cgcaatggtt
     9301 gcgttttggc gaatacaccc tgacaacggg gctgattcct gaaggttatt ggatggacaa
     9361 cacatatgat atttccgccg atttctcatg gtttatctat tcttctgggg atgatgtacg
     9421 tctgctgact tcaagagata atggtaaaac atggcaaacg tctattatca cccaatcagt
     9481 tcagccgata cgttatcgaa aagcggtatt ttatggcaac gatggtctat ttatctgtac
     9541 atcagccagt gggatgtctg cagaaacttt gtacatttac actactatgg atggcggaat
     9601 cacttggata aaaagtggaa gtacaatgat caatcagcca gtacaaaatg tcagcttcgt
     9661 tacaacttct ttaggttttg tttcgacacg tgaaagtttg ttttatacaa acaacggagg
     9721 acgctcattc aaagaatcag ttctgtcgat tccggaaaac tatcagacag gggggataga
     9781 tctttttcaa tcaccaaatg aagttactca aataagcgcg aatacgttag agacaaaatt
     9841 ttatttgttg aaaaaaggac caatcgatgc agggaaaatg tttgcctgcc tcttccgttc
     9901 aactgataac ggggaaacat ggcaattcgt tcagcaacta tcgcaagtag aacaaggaga
     9961 ttaaagaaga gagattgtga gcctgacgtt cagcaccgag tcacaagaaa caaattcaaa
    10021 aaacagcttc tcatatttgt tgaatagtgt aactcgtgat tcggagttga caagagcaac
    10081 aagaagcgat tttgaagtat agcttctcgt attttacaag atcgtgtctt attgccgata
    10141 ggttgtctct tgaacaccgt ttattcgtaa aaagggattg tgacaagtct tttgtcacta
    10201 cttaccgaac gaaactgaag gagctatcca aaaagtatca ttagtattat tctttgtgtt
    10261 tctagtggca ccactagtac atttaaaaaa gctatcgatc atggactaat catacatgat
    10321 cgatagcttt ttagataatg tttgttacgc tatctgaaat tgactattat aaagactggc
    10381 ataaagcgtt ggtgattgga gtagttcaga atgtgttcct ttttcgacga tatcgccatt
    10441 cttcattaca aggatcatgt ctgcattttc aatcgttgac aaacggtgtg caatcacaaa
    10501 acttgtccta tcttttgtca cagcttccat tgcttgctga atcaatgctt ctgttcgtgt
    10561 atctacgcta gaagttgctt catctaaaat aacgacagga ggattcgcta gaatgactcg
    10621 agcaattgtc aataattgtt gctgtccttg agataacgtg ccgttttcgc tagaaatgat
    10681 cgtatcatat ccgtctggca gtgttcgaat aaagtgatca cattgtgcga tttcagccgc
    10741 ttcgataatt tgttctcttg tggcatccat tttcccgtag gcgatattat cagctaccgt
    10801 tccttcaaac agccaggtat tttgtaaaac catcccaaaa gaacgacgta gttctccacg
    10861 tgaaagcttg gtaatatcaa gaccgtcgaa ttcgatcttt cctttattca gttcataaaa
    10921 acgcatcagc agattgacta aagtcgtttt tcctgcacca gttggaccaa cgatcgctac
    10981 cgtttgtttt ggctttactg aaaaatcgac ttgattcatc aatattttat ctgatgtgta
    11041 accaaactgt acatttttga aagctatttt tcctttcggt tgttctaaaa gtcttgcatc
    11101 tggaaggtct ggaatttcat ctgtttcatc taatatctca aatatcctat cgattgctgc
    11161 cattgcggat tggatcgagt tgatcacata agaagcggta gaaattggtt cagaaatctg
    11221 attgatgtac tgaagataag cttgtaagaa gccgatcgtc acaccacctg atagaacaag
    11281 catagcaccg aataccgcac ttatgatgaa ggcgagttga ttaatgaaac gaatggccgg
    11341 atagatagca cagttcataa attgagcacg tttaaaagcg ttgtattgct ttttatttac
    11401 ttcgtgtata gcagcttctg catgagcttg ctgattgaat gttttgacca ctagattacc
    11461 agctaagatt tcttccattc gattatttaa aacacccagt tcatactgat tctgttcaga
    11521 aatctgtttg tttttgttcg caatcttagt tgtaacaatt gaactgccaa cgatcagcaa
    11581 taagacgagc aaagtaagct tgctttcgat ataaaacagc atcagaccag cagcagctat
    11641 tgttacgaca gaagtaaaaa attgattgat acctgtcaat aacacttgag aaagttgatt
    11701 gagaccggta gttgtccgac taatgatgtc acctacttga tgattgtcaa aaaacgacat
    11761 cggtaatgtt ttgaattttt ttgtgatttc ttttcgcacc ctcaatgtga tcttttcgct
    11821 taacgctgcc attgtccgtt cctgaataaa tgaagtgatg ctgcttaaga ctgaacagcc
    11881 gatcagcaat aagacaggga acaacaatgc ttgtttgata tcactgaaag aaagctgtcc
    11941 aatacctgtt tttcttattt ctgataagag atcatcgatc ccgattccca tgatcaaggg
    12001 catggctacg actaataggt tcccgaataa actgcataga aacaatccgt aaaaatattt
    12061 atgttcaggt ttgatcattt tccaaaaacg tatcaaagca cgcgtgttca acgttttttt
    12121 cttttccatc gtatcccttc tttctatatt ccttgtgatt ttgcaaactc ctgataagtt
    12181 tggcaattag acagtagttc ttcatgagta ccttgtccaa cgatttttcc ttcatctaaa
    12241 acaatgatgt tatctgcttg ttggattgta ctgaggcgtt gagcaacaat cacgagagtt
    12301 ttctccgaca tgtgtttatg aagggctgct cttaatgctg catctgtttt atagtctaaa
    12361 gcggagaaac tatcatcgaa tacatagatc tctgctggtt tgatcaacgc tcgagcaata
    12421 catatccgct gtttctggcc ccctgaataa ttagaaccgc cttgagcaac aaagctgtcc
    12481 aatccgtcag gaagggtgtc cactagttct tctaattgag cgatcttgat cgctttagac
    12541 aactcttctg ttgaggcata ttcatttccc atctgcaagt tgcttaaaat tgtcccgctg
    12601 aataagaaag ccttctgagg gacataactg atgtgggagc ggatctgttc ttgagtcatt
    12661 tgagtaagag gaataccacc aagggttact tttcctatcg tagggtcact aagtctcaag
    12721 agaagtttcg ccaaactact ttttccagat ccggtcccgc caacgatcgc tgttgttttt
    12781 cctttaggta ttgtgaaatc aatatcttct agaactggat cagagatttc agtatattta
    12841 aatgtgacat gttcaaaagc agctgcattt gtctgatcaa aggttggttc agctactgtt
    12901 ttgatcgtct cttcctcaat ttcgatctct gcttgcagta tttcttctat ccgtcgtaat
    12961 gaggcaaacg atttagggat aatgaccaat accatagctg cgatcatcaa gtaagcaagt
    13021 gtcagcatag agtattcgac tacagcagtc acgacaccga tttgtaatgc tccttctgct
    13081 acaagatttc cgccaaacca caaaatagaa gcataaacga ttcccatgag caaaaaagca
    13141 attggagtga cccaagcaaa tattcggttc actttgatca tttgattagc atagtcagca
    13201 aagctcttat tcgttcgctt ttcttcatat gattgattat caaaggcacg gatcatatta
    13261 atcccagtaa agaattctct caatgtgatg gtgatccgat ccatttttgg ctggatactc
    13321 aaagataaag gtgcccctcg tttcatcagt cgataaacaa tccatacata aaaaacaaca
    13381 gagatcaatg taacccatgt tagtaataga gaataacgaa acatcatgag aatggtgaaa
    13441 acgcaaataa ccggtgctgg taagatcatt tggcaaaata aaacaatcat ttgctggaca
    13501 ttatcgacat cattcgtcat tcttgtcaaa agagatccag aactaaaacg actgatatcc
    13561 ttcattgaca gctgttggaa tttacggaaa tatttctccc gcgtttggaa tccaaaccta
    13621 gcggaagcca atgctgaaaa gtaactagag gcaactgcag cgattgttcc tagaaacgct
    13681 acaagcagca tttcaactcc aatttttttt ataactgagg cttcgccact tgctatacct
    13741 gtgtcgatca gctttgctac taattttggt acacctaatg taccggcgat ttctaaacag
    13801 atcatcacaa aggtaaggag agcgagtcct ttgtttttct gtaagaatgt ttttaagata
    13861 gacatattat tcctcctttt ttcctgatac ttttcgattc tataccttca agtaacttga
    13921 aggtcaatag aaaaaagaat aaactgaaaa agaggtgaga aaaaatgaaa aaaatgtata
    13981 cgattggcca agtatcggaa ctatttgatt tgccaaaatc aacgatccgc tattgggatg
    14041 aacaaggatt aatccgttca agtagacaag aagagaatga ttatcgattg tttgatatcg
    14101 atgatatttt tatgctttat gatatcgttt tttatagaaa attagatata ccgatcaaac
    14161 agatgaagaa tttatatgga aaaacattga cggagttata tgagacactt gatgaaacag
    14221 aaagaagaat ccataaagaa ctggtggtga tgaaacaaaa acaaaaagag ataagggagc
    14281 aaaaaaaaca gttgaaattg atgatcgata caaacgagga agaatttcct gtagaagaga
    14341 tcccttttga ctgtatgatc tcaacggagt ttgaagatat tgtggaaatc aaaaaatttc
    14401 tacctaacta ttcaagcttt gggatgatga gtatgccagc ttccagttca cagacgatgt
    14461 atggattttt tatcgaccct agtgaagtac atttatttac tcaagacgtg atttgggaga
    14521 aaaaagatac ggctgtttat cgtcgattct tattgaaatc cgagatgaac catgcggaac
    14581 gtaataatat tttagaaatc agagaaagaa tgtatgaaaa aggatggaaa acaggagaag
    14641 tgatcggcca gtatctattg acgaatacgg atgaaaacaa tatcagaact gaatattatc
    14701 atgcatggat agaaatgaaa aaataggaaa tatgtgttta agataactcg aaaatgatgg
    14761 ttgttttagt tataaagaaa aattttctgg ttcgattacg ggtttaatgt gggggattat
    14821 tagtcgtgga tagatagaat tttagttttt tagaggaaat atgtctttaa aaatggaatg
    14881 gttgcaattc ttagcgatat atacgatgta attgtcctct taaaaaagta tggaaaaata
    14941 attgtattgg gtgttatgta taattcatgg tgaactgaga ctaatgagaa gaaaaattca
    15001 tattgttatg agtgagattg tatcagatga gaatgataaa ttaaataggt gcataaaagc
    15061 attacactta aaatacattg ttgcttacat tattacaata tgttaataag gcaaaaataa
    15121 atagatggat tgaataaaca tattttttaa tcaaatagtc cctactgtga ctttcctcag
    15181 ctatcggtga tactaccgat agcatttttt tgcttataaa ataattataa gtatttagag
    15241 atttttcttc taacattctt caa
//
LOCUS       DACDKJ010000070        15230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDKJ010000070 DACDKJ010000000
VERSION     DACDKJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.070"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IV328_002358"
     CDS             278..787
                     /locus_tag="IV328_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8938463.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IV328_002359"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IV328_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8938464.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IV328_002360"
     CDS             2322..3302
                     /locus_tag="IV328_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8938465.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IV328_002361"
     CDS             3424..4842
                     /locus_tag="IV328_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8938466.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IV328_002362"
     CDS             4913..6382
                     /locus_tag="IV328_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8938467.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IV328_002363"
     CDS             6392..6859
                     /locus_tag="IV328_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8938468.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IV328_002364"
     CDS             6895..7767
                     /locus_tag="IV328_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8938469.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="IV328_002365"
     CDS             7848..10382
                     /locus_tag="IV328_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8938470.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="IV328_002366"
     CDS             10538..11311
                     /locus_tag="IV328_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8938471.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="IV328_002367"
     CDS             11304..12095
                     /locus_tag="IV328_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8938472.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="IV328_002368"
     CDS             12105..12596
                     /locus_tag="IV328_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8938473.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="IV328_002369"
     CDS             12614..13024
                     /locus_tag="IV328_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8938474.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="IV328_002370"
     CDS             13028..14428
                     /locus_tag="IV328_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8938475.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="IV328_002371"
     CDS             14451..14825
                     /locus_tag="IV328_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8938476.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DACDKJ010000071        14504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDKJ010000071 DACDKJ010000000
VERSION     DACDKJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.071"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(308..670)
                     /locus_tag="IV328_002372"
     CDS             complement(308..670)
                     /locus_tag="IV328_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938477.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(744..1124)
                     /locus_tag="IV328_002373"
     CDS             complement(744..1124)
                     /locus_tag="IV328_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8938478.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1206..2381
                     /locus_tag="IV328_002374"
     CDS             1206..2381
                     /locus_tag="IV328_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8938479.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2458..3042)
                     /locus_tag="IV328_002375"
     CDS             complement(2458..3042)
                     /locus_tag="IV328_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8938480.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3243..4241
                     /locus_tag="IV328_002376"
     CDS             3243..4241
                     /locus_tag="IV328_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8938481.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4353..4862
                     /locus_tag="IV328_002377"
     CDS             4353..4862
                     /locus_tag="IV328_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8938482.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4892..5551)
                     /locus_tag="IV328_002378"
     CDS             complement(4892..5551)
                     /locus_tag="IV328_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8938483.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5553..6317)
                     /locus_tag="IV328_002379"
     CDS             complement(5553..6317)
                     /locus_tag="IV328_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8938484.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6325..7002)
                     /locus_tag="IV328_002380"
     CDS             complement(6325..7002)
                     /locus_tag="IV328_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8938485.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7039..8424)
                     /locus_tag="IV328_002381"
     CDS             complement(7039..8424)
                     /locus_tag="IV328_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8938486.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8634..8718)
                     /gene="ffs"
                     /locus_tag="IV328_002382"
     ncRNA           complement(8634..8718)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV328_002382"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="IV328_002383"
     CDS             complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="IV328_002383"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8938487.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9363..10220
                     /locus_tag="IV328_002384"
     CDS             9363..10220
                     /locus_tag="IV328_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8938488.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10301..10636)
                     /locus_tag="IV328_002385"
     CDS             complement(10301..10636)
                     /locus_tag="IV328_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938489.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10703..12073)
                     /locus_tag="IV328_002386"
     CDS             complement(10703..12073)
                     /locus_tag="IV328_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8938490.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12151..12687)
                     /locus_tag="IV328_002387"
     CDS             complement(12151..12687)
                     /locus_tag="IV328_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8938491.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12996..14477
                     /locus_tag="IV328_002388"
     CDS             12996..14477
                     /locus_tag="IV328_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8938492.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4470 a         2887 c         2355 g         4792 t
ORIGIN      
        1 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
       61 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      121 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      181 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      241 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      301 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      361 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      421 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      481 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      541 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      601 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      661 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      721 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
      781 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
      841 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
      901 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
      961 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1021 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1081 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1141 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1201 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1261 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1321 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1381 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1441 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1501 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1561 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1621 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1681 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1741 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     1801 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     1861 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     1921 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     1981 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2041 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2101 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2161 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2221 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2281 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2341 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2401 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2461 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2521 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2581 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2641 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2701 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     2761 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     2821 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     2881 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     2941 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3001 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3061 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3121 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3181 aaacattttt gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt
     3241 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3301 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3361 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3421 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3481 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3541 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3601 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3661 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3721 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     3781 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     3841 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     3901 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     3961 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4021 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4081 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4141 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4201 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4261 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4321 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4381 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4441 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4501 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4561 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4621 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4681 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4741 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     4801 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     4861 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     4921 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     4981 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5041 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5101 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5161 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5221 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5281 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5341 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5401 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5461 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5521 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5581 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5641 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5701 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     5761 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     5821 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     5881 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     5941 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6001 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6061 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6121 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6181 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6241 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6301 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6361 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6421 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6481 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6541 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6601 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6661 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6721 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     6781 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     6841 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     6901 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     6961 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7021 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7081 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7141 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7201 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7261 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7321 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7381 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7441 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7501 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7561 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7621 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7681 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7741 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     7801 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     7861 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     7921 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     7981 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8041 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8101 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8161 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8221 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8281 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8341 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8401 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8461 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8521 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8581 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8641 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8701 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     8761 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     8821 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     8881 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     8941 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     9001 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     9061 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     9121 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     9181 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     9241 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     9301 tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca
     9361 agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag
     9421 catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag
     9481 attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc
     9541 aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt caagaaaaaa
     9601 gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc
     9661 aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag
     9721 atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta
     9781 tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc
     9841 attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag
     9901 agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt
     9961 actatacaaa agatactgta aaagatagta agttgatcaa aagttacata gaactgatca
    10021 aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct
    10081 ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc
    10141 aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat
    10201 tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac
    10261 cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa
    10321 aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag
    10381 agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac
    10441 tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga
    10501 cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg
    10561 atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt
    10621 ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa
    10681 aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta
    10741 ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc
    10801 atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt
    10861 gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc
    10921 taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta
    10981 aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa
    11041 gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct
    11101 cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt
    11161 gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata
    11221 tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg
    11281 attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga
    11341 tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt
    11401 ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag
    11461 attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac
    11521 aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg
    11581 gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa
    11641 tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag
    11701 cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg
    11761 gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc
    11821 cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg
    11881 gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta
    11941 gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg
    12001 atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg
    12061 cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa
    12121 ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc
    12181 caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc
    12241 ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga
    12301 cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag
    12361 cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa
    12421 aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta
    12481 gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt
    12541 gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt
    12601 aattgacttc attccatatt cttccacgta taccatactt ttgaacagca aatattcttt
    12661 actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa
    12721 taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata tttagttata
    12781 tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg
    12841 aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa
    12901 ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta
    12961 actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag
    13021 agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca
    13081 atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac
    13141 tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa
    13201 ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc
    13261 taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca caaactgctg
    13321 taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga
    13381 caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct gtaaaaaatt
    13441 gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag
    13501 atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga
    13561 acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt
    13621 taactttatt catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa
    13681 tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt
    13741 tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt aattattttt
    13801 tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt ggttttttat
    13861 tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact tacttaactc
    13921 aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat tttcttgtgt
    13981 tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct acactttgct
    14041 ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa ttaagagaaa
    14101 aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa taccttagtc
    14161 aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc catttgcaga
    14221 taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag ttatttcgca
    14281 aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt acagatcagc
    14341 atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac aatacagtgt
    14401 tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt tatgagaaca
    14461 aaacaaagag gatgtgacaa aattcttgtc acatcctctt attc
//
LOCUS       DACDKJ010000072        14379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDKJ010000072 DACDKJ010000000
VERSION     DACDKJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.072"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            83..1081
                     /locus_tag="IV328_002389"
     CDS             83..1081
                     /locus_tag="IV328_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8938493.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="IV328_002390"
     CDS             1105..1389
                     /locus_tag="IV328_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938494.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="IV328_002391"
     CDS             1470..1868
                     /locus_tag="IV328_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8938495.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="IV328_002392"
     CDS             1878..4421
                     /locus_tag="IV328_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP8938496.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="IV328_002393"
     CDS             4443..6785
                     /locus_tag="IV328_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938497.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="IV328_002394"
     CDS             6801..8975
                     /locus_tag="IV328_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP8938498.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="IV328_002395"
     CDS             8965..9387
                     /locus_tag="IV328_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8938499.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10330..11241
                     /locus_tag="IV328_002396"
     CDS             10330..11241
                     /locus_tag="IV328_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938500.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11728..11994
                     /locus_tag="IV328_002397"
     CDS             11728..11994
                     /locus_tag="IV328_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938501.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12066..12407
                     /locus_tag="IV328_002398"
     CDS             12066..12407
                     /locus_tag="IV328_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938502.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12462..12881
                     /gene="ssb"
                     /locus_tag="IV328_002399"
     CDS             12462..12881
                     /gene="ssb"
                     /locus_tag="IV328_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8938503.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12958..13254
                     /locus_tag="IV328_002400"
     CDS             12958..13254
                     /locus_tag="IV328_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938504.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13269..13700
                     /locus_tag="IV328_002401"
     CDS             13269..13700
                     /locus_tag="IV328_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938505.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13882..14187
                     /locus_tag="IV328_002402"
     CDS             13882..14187
                     /locus_tag="IV328_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938506.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         5077 a         2230 c         3047 g         4025 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagcc ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacagaaact tgcgagagag ttggagtaaa acaagtgtag
    10201 aggaaacttg aagacttgtt cgaacacaaa cttacaagag aggggagtaa agcagttgca
    10261 gaggaatcag gaagatttgt tcgaacagaa acttgcaaga aagcattatt aaattgaagg
    10321 tgatgaaata tgaagtattt attgcatggc tattttattg attattctac tcgcaatagt
    10381 agtactttag cgattctatg tttgcttcat gaattgagag tggttactag aaaacagcta
    10441 tacaaactat tgaattttga cagatcaatt agtaaacaag gagtaacgaa tgctttagtg
    10501 agattatatg aaaaacagtt aatcgatcga aaaaaatatg gaacagaaat gtattattat
    10561 ttgacgaaag agggacatca aagcataggt gggtattata cgttgccgaa agttcctgaa
    10621 tataatttga atcaccattt acaaattaac aattatttga ttaaaggaat ggcattggca
    10681 aaagaccacc cacatttaaa acggattatt tcagaaagac gtaccgtcta tgaaacgaag
    10741 gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc cagattttat ttttcgtttt
    10801 cgagacaaag aaaatcatga gatcaattgg tcctttgaaa ttgagctaac gttgaaaaca
    10861 cgtcgtcgct accgtgaagc gatttttccg aaatatattc gtgagttgcg aacacgtcct
    10921 gaatcacatt taatttatgt gacacctagt ggcgcaattc aagaagaact tcatgcgttt
    10981 aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga gtcaacaaga attgtttgag
    11041 cggttgcata ttttttcagc tttagagttt gaagagggat taaaagcatt attagagaat
    11101 gatccagcag tcaattggcg gacagtagaa cgagaaaaaa tttatccgaa tggcgctgtt
    11161 caaagtcgtt tgaatttatt tgaagaatca actgaaaatg aggaaaaacc aaaagtgaag
    11221 tttacgcctg aagtattgta aaagagggtg ttcgcttcgc tctttttttg tgccagtttg
    11281 ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt ttaggaagga ctaacttgtt
    11341 tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc ctaatcgtat agcagtcatt
    11401 cggcgagtat aacctaaaac ataacctgcc aattgccaag ggtaaaatgc acgctgcgcg
    11461 cccttggcaa tcgtcagaac atgttttcga gccggttatg ccgaacgacc gctatacgaa
    11521 aagggcctct aaacagaagt ccactttgat ccttgacaac tgaaacccaa aatcaaacaa
    11581 gcaggccttc gtctaaaaac gagccgaatg aagaccttgg gggttgcacc cccaaacccc
    11641 cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg cgaaaaaatc aaataaaaca
    11701 ttctattcaa gaaaggaaga aacaaccatg acaactaaaa acgagcaact gaaagaacag
    11761 gcaagaaaag agttgcaaga aagaggtttg attattgagg ggagctttga aggagatttt
    11821 gaaacctata ttggttgtta tgcaagaccg ataaacaaac ctacagcctt agacccaatg
    11881 aacgaacaag aagtaaaaga acaagaaaaa catgcagtga atggttttcc gcaagatttc
    11941 actgaatggt atgagtggga aattaacaac gggaagcttg aaaacttctt ataaaagtca
    12001 gatgacgtgc aaagagaatg attctctttg tacggtaaaa atataaagaa agaaggaaaa
    12061 agaaaatggc aaaaaacaca ccttatcaag taggagacat tttttatagt agttggggct
    12121 acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt aacagaaaaa acagcatggt
    12181 ttcagcctat tcaaaagaaa acggtcgagc attattcgtc catgagtgga aaagttgttc
    12241 caatgaaaga caagtttata gagtatcctt tagcaaaaac taaagacagt attgtaaaaa
    12301 aacgaattga tccagaaacc tatcctgata tgttatgtgg ttatcgtagt tgggacacgt
    12361 ttcatagata taaaaatcaa gctgtttatg aaagtagtta ttattaaggg gatagtttcc
    12421 cctttcaaaa ataataaata gaagaatgga gagaaaaaat catgatgaat tcagtgaatt
    12481 tagtaggacg gttaacaaaa gaagtggatt taaaatacac accaaaaggc aatgcgacag
    12541 gcacatttat tttagctgtt aatcgaaatt atacgaatgc aaacggcgag cgtgaagcag
    12601 attatatccg ctgtgtcatt tggcggaaag ctgcggaaac tttagcaaaa tatactggaa
    12661 aaggaagttt gattggcatt aatggacgtc ttcaaacgag gagctatcag aatcagcaag
    12721 gacagaccat gtatgttacg gaagttctag tgacagattt ctatttgtta gagagtaaag
    12781 aaatcaatga gcaacggtca aaatcaatgg cgaataatca aaataattct tcaattccag
    12841 gaaatgagat tcaaatttca gatagttatt tgccctttta agtgaaaaat aattttgttc
    12901 tatacctgat ttcttcaggt atagaacaaa aaataaatag agatgaggcg attttttatg
    12961 aaaatcattg acagttacga tcaacgaacc atgcaatatt tgggctcgtt tgattataca
    13021 gatgaaaata ttattaatta tgttgcaagt ttacctcctt atcaaagcgt tcgcttggta
    13081 gattttgcaa gtgatgaatt ggtattaaca acgatgggca actttttaga ttatgtcgga
    13141 gacaaaaaat ggttggaaaa aattttgtct aaattgattg cgaaacaaac aggagaagaa
    13201 cccataaatg cgattcaatt gttcaatcga tatgaacaag aaaacaatga ttaaagagga
    13261 gagaaaaaat gaaaagatta aaacgaagta tttttattgt gctagcattg attgattttg
    13321 ttgggacagg ttgtgaagca attgacgttc aaaaagctcc cttatcggaa ataaaacaag
    13381 caataacaaa acaagatgaa gaacaaccgc taaatacaca agtagaaata gtgaatcaaa
    13441 ataaaccgag tttttctgaa gaagattttt cgttagaaaa tggaaccagg caattttttt
    13501 cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc acttttgcat aaaagcagga
    13561 tgccaacaga agaacgagac cgattataca ttaagccaac tggctggaaa caaaagaaaa
    13621 tgaagaatgg cgattatctt tataatcgtt gtcatttgat tggctatcaa cgtgcgacaa
    13681 gtttccccat aagtatgtaa tacgaaacgg gaggattaat cttaagatta atcacaacta
    13741 acctatttga cagttggaat gaaagctatc atgtctgctg aatcaactgg tctgatactc
    13801 ctacatgcaa taaagtctac taggaagtta ttgtatgaag ctaggtaaag gcggcagaac
    13861 aatttctaag tgtggaatca tatgaagttg gatatgccgg gacgctataa attactcagg
    13921 gtgagactgt ttttaattaa actgacgaac ttgcgaatgt acgggtctat aaccgactta
    13981 tattcgaaag atataagatt gcgaatgcaa agaacattat cgaaaagtaa gaatgttggt
    14041 catgaaattc gagatggaag actagcggtg tatctgtcaa gagttggtaa aacatgtttg
    14101 attgattcag atgagagtgg gaatgcgaca aaacgtttta cagaagaaat agagcaacta
    14161 gcagaactga atatacgata tttttaaata ctttagcgga gaataaaaaa atcctctaca
    14221 gaaaactacg gagggcaaca tgtgctattg tttcaacaat acatttttaa tttttaccat
    14281 aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat taaaaatgaa aaaatggaag
    14341 cggttactta ttcttttgaa tctaagataa aaattcaaa
//
LOCUS       DACDKJ010000073        14161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDKJ010000073 DACDKJ010000000
VERSION     DACDKJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.073"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IV328_002403"
     CDS             66..767
                     /locus_tag="IV328_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8938507.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IV328_002404"
     CDS             764..1885
                     /locus_tag="IV328_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8938508.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IV328_002405"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IV328_002405"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8938509.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IV328_002406"
     CDS             3264..4172
                     /locus_tag="IV328_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8938510.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IV328_002407"
     CDS             4207..4479
                     /locus_tag="IV328_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8938511.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IV328_002408"
     CDS             4490..5536
                     /locus_tag="IV328_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8938512.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IV328_002409"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IV328_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8938513.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IV328_002410"
     CDS             complement(8547..9218)
                     /locus_tag="IV328_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938514.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IV328_002411"
     CDS             complement(9211..9705)
                     /locus_tag="IV328_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8938515.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IV328_002412"
     CDS             complement(9725..10945)
                     /locus_tag="IV328_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8938516.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IV328_002413"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IV328_002413"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8938517.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14148)
                     /locus_tag="IV328_002414"
     CDS             complement(13021..14148)
                     /locus_tag="IV328_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8938518.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4575 a         2602 c         2927 g         4057 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatcttcg ccgtttgtat aattaagcga gacaaataaa aaagccattt atgttacact
    14161 c
//
LOCUS       DACDKJ010000074        13919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDKJ010000074 DACDKJ010000000
VERSION     DACDKJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.074"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(221..1423)
                     /locus_tag="IV328_002415"
     CDS             complement(221..1423)
                     /locus_tag="IV328_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8938519.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="IV328_002416"
     CDS             complement(1426..1620)
                     /locus_tag="IV328_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8938520.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="IV328_002417"
     CDS             complement(1667..2515)
                     /locus_tag="IV328_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8938521.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="IV328_002418"
     CDS             complement(2720..3070)
                     /locus_tag="IV328_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938522.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="IV328_002419"
     CDS             3665..4111
                     /locus_tag="IV328_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8938523.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="IV328_002420"
     CDS             4735..5727
                     /locus_tag="IV328_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8938524.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="IV328_002421"
     CDS             5743..6627
                     /locus_tag="IV328_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8938525.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="IV328_002422"
     CDS             6676..8283
                     /locus_tag="IV328_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8938526.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="IV328_002423"
     CDS             8284..9768
                     /locus_tag="IV328_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8938527.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="IV328_002424"
     CDS             9945..10937
                     /locus_tag="IV328_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8938528.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="IV328_002425"
     CDS             10989..11858
                     /locus_tag="IV328_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8938529.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="IV328_002426"
     CDS             complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="IV328_002426"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8938530.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4258 a         2358 c         2875 g         4428 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag
    13861 tccgatatgc ggcgcgttct gtgcgccgca tattttttta gttttcaaag atcagacct
//
LOCUS       DACDKJ010000075        13887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDKJ010000075 DACDKJ010000000
VERSION     DACDKJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.075"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            56..841
                     /locus_tag="IV328_002427"
     CDS             56..841
                     /locus_tag="IV328_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8938531.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1550..1909
                     /locus_tag="IV328_002428"
                     /pseudo
     CDS             1550..1909
                     /locus_tag="IV328_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2204..3337)
                     /locus_tag="IV328_002429"
     CDS             complement(2204..3337)
                     /locus_tag="IV328_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8938532.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(3328..3996)
                     /locus_tag="IV328_002430"
     CDS             complement(3328..3996)
                     /locus_tag="IV328_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8938533.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(4452..5438)
                     /gene="lacD"
                     /locus_tag="IV328_002431"
     CDS             complement(4452..5438)
                     /gene="lacD"
                     /locus_tag="IV328_002431"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8938534.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(5451..6377)
                     /gene="lacC"
                     /locus_tag="IV328_002432"
     CDS             complement(5451..6377)
                     /gene="lacC"
                     /locus_tag="IV328_002432"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8938535.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(6390..6905)
                     /gene="lacB"
                     /locus_tag="IV328_002433"
     CDS             complement(6390..6905)
                     /gene="lacB"
                     /locus_tag="IV328_002433"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8938536.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(6924..7352)
                     /gene="lacA"
                     /locus_tag="IV328_002434"
     CDS             complement(6924..7352)
                     /gene="lacA"
                     /locus_tag="IV328_002434"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8938537.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(7372..8145)
                     /locus_tag="IV328_002435"
     CDS             complement(7372..8145)
                     /locus_tag="IV328_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8938538.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            8310..8789
                     /locus_tag="IV328_002436"
     CDS             8310..8789
                     /locus_tag="IV328_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8938539.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            8846..9148
                     /locus_tag="IV328_002437"
     CDS             8846..9148
                     /locus_tag="IV328_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8938540.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            9170..10627
                     /locus_tag="IV328_002438"
     CDS             9170..10627
                     /locus_tag="IV328_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP8938541.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(11220..11972)
                     /locus_tag="IV328_002439"
     CDS             complement(11220..11972)
                     /locus_tag="IV328_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8938542.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(12187..12423)
                     /locus_tag="IV328_002440"
                     /pseudo
     CDS             complement(12187..12423)
                     /locus_tag="IV328_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(12433..13391)
                     /locus_tag="IV328_002441"
                     /pseudo
     CDS             complement(12433..13391)
                     /locus_tag="IV328_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(13597..13821)
                     /locus_tag="IV328_002442"
                     /pseudo
     CDS             complement(13597..13821)
                     /locus_tag="IV328_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
BASE COUNT         4108 a         2888 c         2383 g         4508 t
ORIGIN      
        1 aaaagcaaaa aaaaaagaaa aaatcttatt cttgataaca agctacgtga cgtatatgcc
       61 taacaaaaat agaacttttg aatcaatctt aaatgaaggt ttaaccacag aaccggcatc
      121 cattattttt gatgaagtta tcgatgacgt tgaagatagt aagtatcata aaaatttaaa
      181 agaacttttt tattttgcac tcaacaatgg accaagtttg ttcactggta tatcagtacc
      241 tgatagtgat aatccattaa ttatggcaaa aatttactta acaaaatggt gtaataaata
      301 catcagggat aggaataacc cagcattaaa aaaacctctt aaaacatttg gcgaaaaaga
      361 tgctgcttta actactagag tagcagcaaa tgctaatatt gatgatcaaa aagtattgaa
      421 tgactacttg cgtggacatt ttttatatat gagcgctgag aacatgaacg gaagtatttt
      481 agaggaatat ttagccgaag tgttagaacc tgaaggttgg atttggtgcg ccggctctgt
      541 atatcgagct gttgatttct gttatttagg aacctcacca attcttttac aagtgaaaaa
      601 taaatacaat acagaaagca gttcgagttc agctataaga gtaggaacaa caattagaaa
      661 atggaatcgt ttaaataagt cgacaaaaat ttctggtttg gatagcccta tccccaattg
      721 gaaagcatta atagaaatga ctgaagctag taaggaactg gcagctaagt taactgaaaa
      781 tagttattta gcttatatta atgaaaaatc cacacgagaa ttatggacac tcgatgacta
      841 acaatatcct tattagtatc tatccacgat acatggaaaa aattataaac aaacaaaaaa
      901 actttgaatt tagaaatttc tatcttacct cagatcctta taatatcttt tggatttacg
      961 aaacaatccc caccaaagct ttaaagtata aattgattgt aaaaaatcca attactaaac
     1021 ttaagaaaaa tcaacactac tttttaggag acgatgagtt tgaacaatta gtttaaaagg
     1081 gaaaatatgc ttctatcatt acaagctcgt ttggtgtaag atgggtataa gtcatttatg
     1141 ttcactctcc ttgtatgctt tagcgggtat tacaatttga gtgtaacata aatggctttt
     1201 ttatttgtct cgcttaatta tacaaacggc gaattataaa taactcataa aaaatatttt
     1261 gactttcaaa atcacttttg ctaccagaag ttgaggcgaa gtgttttaag catagttgta
     1321 ccctagtttg accgttaaaa ataaataacg atcgctagag tgcaactatg tttgtttgcc
     1381 ctaccgtcag cgtcggcttg gcctctcaca tccattaccg tcgggattat ctccgttaca
     1441 tacccttaca aaatacgccc tgtaagcatt gcatgaaacc accgtgcatt tgcaccgtat
     1501 ttccgcccta gccgttcaaa tcggtcgcta ccaccattca gaaacacgtg cggtaatgga
     1561 aggtttggag gaagctatcc agaccactaa cgattgtccc tatcgtcgta cgtttcactc
     1621 agatcaaggt tggtcgtatc aaatgaaagc ttatagaaac aaattgaagg agtacaaaat
     1681 ctttcaaagc atgtctcgga aagggaactg cttagataat tcacttatgg agaatttttt
     1741 tggcttatta aaacaagaga tttttcacgg gaaagtttat aactgtttcg aggaattaaa
     1801 atcagccatc gatagctata tctattacta caataatgaa cggataaaac agaaattaaa
     1861 ctggcaaagt cccgtccaat ttcgtaaaac aaccgttacg gttgtgtaaa aatatcattt
     1921 tagagcatga aaaaaagagt ggatttctcc actccaaaaa agtctaactt ttgggggtca
     1981 ctacattttt gatccttttt atttgattaa ttatatccag ctggataata attttggggg
     2041 caacatttct atcttactat tatcttccta aagtagtaga ctttcttatt gggaaaaatt
     2101 acaacaaaaa ggagtggctt agttgacaac tcctttttgt ttacaaatca attggatatc
     2161 tataaattgt attttctaac gaattttata gacatctgat ttgtcaaatt aagctagaca
     2221 atatgtcttt atctatgcag ctcaaaaata cttccaatgg tgttcgataa tttaatgatt
     2281 ttcttggaat atggtttctt ttcgaggcaa cggattgaat aaaggtttca tccgtgtccc
     2341 taaagtccat tgttttaggt aagccatcct gcttgagccc atgcactcgg tactgatgag
     2401 aaaaaattga tgcctgtttc tcctgaggaa tgccaattcc ctgatctttt acttcaataa
     2461 tcacgctacc cttttggttt gaaatggtga caattaccag ttttccttca ccatatttga
     2521 ttgcattgtc aactaaattc gaaaccgccc gataaatcca acgatctcgt ccatgaatca
     2581 gggttggttg atcttcagta aacgcaaaca aaagattcgg ataattcatt ttgtaggcat
     2641 cacaaacttc cgcacagatc agtgaaacgt ctacgacttc cgtgctaaga tcctctgagt
     2701 tattggataa tgtcaaaata tcgtcaatac tgtcggtcat ccgatctaaa agcattaaat
     2761 ttccttgatc ggcatgttgc cgttctattt ccagattcgt acgcaaaata gcgatggcat
     2821 ttttctgctc gtgggtcaga taactattta atcgtcgata atcctccaaa tgaccatcga
     2881 acttatcctt tagcttatta aaagccgcat ttaatgcctc gtccttcaca gtatttttag
     2941 gaacttgatc aataaacgtt aaatcacgcg taatcgctaa gatttgatta ctgtaaattt
     3001 tgcgtaaaac caaccaaaga ataaacgtta ataacatgac ggcagaacaa ataaccagca
     3061 ccatcgctgg taaatattta aatagttcgt cctggtaagc tttatctatc atcgaaatag
     3121 tcataaattg attgttggaa gatttcgaag cttccatctg cgtctctgct tgaaccgtct
     3181 taccctcttc cataaaataa acatcttcac ctaaggtaga tgcgatactg attccctcca
     3241 aattatttct cagaaagaag aaactcacta aaatcacgcc tgtcaccaac agtgaaaaag
     3301 ccagcatcgt taacgtcatt ttaaaattta atagcatagc tggtacccct ttgctcgaat
     3361 ggtttttaag acctcttttt cagctgcttc agccagtttc ttttttagac gagccagatg
     3421 cactcgcaga accgacgaaa aaggatcaaa atcttcatcg tagacatgtt cagaaatttc
     3481 ctcagacgaa acaaccgctg gatgacgctg agcgatgcac agtaagatat caaattcctt
     3541 gggtttcagg accacctctg tcttttgata gctgaccctt cgacctaatg gatcaatctt
     3601 caaagcacca attgaaatct gcggcagcgc acgtccatga taacgacgaa taaccgcgcg
     3661 tattcgtgct tttaattcta gtaactcaaa gggtttgact aaataatcat ctgctccaaa
     3721 atccaagcct tttgctcgtt cttcaatctc atcacgtgca gtaataatga taactggact
     3781 ttcgacttct tcagcacgga gactttgtaa aacagataat ccatcttttt taggcagatt
     3841 taaatctagt agtatcccat cgtatgaatt gatgaaggcc ttctcttctc ctgactcgcc
     3901 atcataagct acatctacag aaatattctc ctgctctagg ccctttttta cggataaaac
     3961 cagctcacga ttgtcctcaa ccaccagtac acgcatgtat attcactcct catacggatt
     4021 tagtaattac acttatgctt tttagcatag tacacgctct tcttcaaagt ccacgttctt
     4081 tttgtgaaga tttgaagaaa ttcatacatt ctgttaaaaa acacataatt aagaatgatt
     4141 ttactgtttg aaaataccta gaatagaaaa agactggaac ctttcctcca gtctcactac
     4201 tttctatttg cttggttttc ttgtatatac ttttcaaagt tcgttggttt atgaccaata
     4261 agcctcgtta gaatactgaa aattgccatc aagtcattaa gtaccacatg taagaaaatc
     4321 tctctaaaga aaaacccctt cgttttaact cacttactat tttagaattc gttcccctaa
     4381 taatgcagca tgattttacg ctttatgtat aaaaaaagac tgcaagcaat tttgcttcac
     4441 agtctgattt cttatgatag tgagcttgtt aacacttcca ccggtgtagc agtttctttc
     4501 aatacggcat ttaattcttc aatattttta cgcccttgcg tattcatcca ctccatcgct
     4561 gcttcttttc catcaacgat atagctttct acaccatctg cccaagtcgc acggccgcaa
     4621 agtacaccat tgaaggttga gccggcattt ttcgcgaagc gcagtgtttc ttggaacaac
     4681 tctgctttta caccggcgct taggaaaata aatggcaggt tagtggcttc agcctgttcc
     4741 ttgaagaagt tagccgcttg tgcctttgtg tacacgactt cgtctgtccc atagccttcc
     4801 acataattca tattgacggg tacttccacc tttaacacat ctacgccata acgtggatca
     4861 gaaaattcct tcatcatatc gttgacttta tgaggtttct taatggcgta ttctttcgaa
     4921 gtcgcatcag agatgttcgc gtcataagaa acaagctcca agaagaaggg taagttttca
     4981 gcctgacatt ctgagccgat tcgttcaata aaggcatgtt tttgattatt gatttcttct
     5041 gcttcatcga catcatagta aagtaggaac ttacaagcat cggcgcctgc ttcttttaaa
     5101 cgtttaactg accagatcga taataaatct ggtaaacggc cgggagttga cgcatcgtaa
     5161 ccagtttttt cataagctaa cagcaagccg gcattttttg ctctaacgtc agctgctggc
     5221 aagccatatt cgggatctaa taaaatcgat gaggcatatt tcgttaattg tgaagaaaca
     5281 atttctttaa attcaacgat ttctgattct tctgcttcgc tgcccttgaa ttttgtgatc
     5341 atcttcttca aagccccgcg ttgatcaatt gctaaggccc caatcactcc atcttcattg
     5401 catagttgct tgatatattg acgtttttct tcgttcattt ccgtatcctc ctacactttc
     5461 tccactaaga tttgatcata tagtttttga taattggcta attggacata gccagtcgtc
     5521 gcttcctgag cattcagcat tcctaaaaca ttaccctgtt ttaacacggc ttcatccgaa
     5581 agaccttgtt gaatcgcagc ggtcaatccg gcaacggtcg catcaccaga gccaactgga
     5641 ttgacgacat caatctttgg aattgtcacg caataaaaag tctcgtgatg tttggcaaag
     5701 gctccgtcgc cccccattga aaccactatc cattcaattt catcaaacat cgaatctgac
     5761 aaggcggctt ttaaatctga taattctgtt gagatttctc gtcctagcag accagccagc
     5821 tcttcggtat tgggttttat tagcttaggc tttacactgc ctcgcaacac ttcccttaat
     5881 gctgtacctg aacaatctaa aacgacaggc tttggctgac aaacttgcag catttgtcga
     5941 taaaaatcag caggcaatcc ttctggcaag cttcctgaaa aagaaatgat ttcggcttta
     6001 acagctaatt tttcgaaatg ttgcaagaac gcccttgctt cttgttcagt aataacaggt
     6061 ccgttttcta ggatttccgt ctgttttccg tcatgtaaaa cagcgatgca attacgtgtc
     6121 tctccgctga taggtaaaaa gtcatgagca atctcatttt gagtcaggtc ctttttaatc
     6181 tcttcaccta aaaaaccgcc gattaaacca ctagcaatca cctccgtatc taattgcttc
     6241 aagatacgtg tcacatttag gcccttaccg ccagctgttt tgcgaacatt tcttactcga
     6301 ttgactgtgt cgagcttgaa ggtttctaat ggataagaaa tatcaatcga tggattcatc
     6361 gttattgtta aaatcatttg tccacccgct taatcgtggt attcgccacg atcccatttc
     6421 tcgataaatt catcgaagaa atgcggatca gactgcttgt cactggcttt ttcgacgtga
     6481 tcgattttgg cgatcaattt tttgttttct tcagtttctt gataatcggc attgatgaag
     6541 gcctctaata tgtcacagat caaaaattct cccgtaatcc gagcgccaaa gccgatcaca
     6601 ttggcattta gttgttcctt cgcatagata gcagtcgtca tatcgcgaac cagcgcactg
     6661 cggataccag gtactttatt gacagcgttg ttaatcccaa cacctgttcc acacaaacaa
     6721 actcctagat ctgcttctcc gctagtcacg gcctctccga ctttttttcc gtagatcgga
     6781 taatgggttc tggtaaagtc ataggtcccc atatccaaca cttcatgtcc ttttgatttt
     6841 aagaaatctg atactgccat ttttgtatct gtgacgatgt ggtcacagcc aattgcgatt
     6901 ttcatttcat tcatccttcc taattacgcc attttattta acatgtcgac acgtacttga
     6961 tgacgaccac catcataatt tgcttgtaga aattccttaa caatattcaa cgccaactca
     7021 tcgccaacaa tttttgatcc aagtgtgatc atccgagaat tattatgttt acgggtcata
     7081 tacgcactgc gctcatccga aacttctgct gctaccattc ctttaacctt tgttgccgtg
     7141 ataaagctgc cagcgccgta gccatcaacc gcgattccta aactaccttc gttttcattc
     7201 agatcatttg cgatcgccaa agttgattcc acaaaatctt ctgaagccgt ttcactctta
     7261 tctactactg taataccttc tttgatcaat tttttcttga ttaattcctt taattccatg
     7321 ccgtcactgt ctgaaccaat tactacacgc atttttgttt cctcctattt tttactgaat
     7381 gattttagtg aattgctgat aataactctt cagctcggct gaaagagtag aatccgtaaa
     7441 taatttgtcg atcttgcgca aatcatagaa attataaaaa tcgctatggt cgaatttgtt
     7501 tgcatctgaa accaaacatt tttctgccgc attatctaaa acaatttttt gcaaggtacc
     7561 ttcttcaata ctgaaattcg aaacctccga acccttcaat ccattcactc cgacaaaagc
     7621 cttcttaata ccgatttttt gaacagcttc attggcaatt gaaccgacaa aggctccagt
     7681 cttttcccga tatagcccac cgatcaaata cagttcaaat ctttttattt cacttaatag
     7741 attgaaaacc ggcagactat tggtaacgat tcggagagat gacggcttga tgtatcgtgt
     7801 cattaattcg atcgttgtac ccgatccaag aaaaatcgtg tcgtcttctt gattgatatg
     7861 agctgccgca tttttagcga tgtattcctt ctcttttgta tgcagcttct ttttttcagt
     7921 atgagaaagc tcatttgctt ctatagaagt cgacatttcc agataatcga ttttttgtgc
     7981 accgccatga acgcgtaaaa gctttttctt ctgcgcgagt tcctctagat ctcggcggat
     8041 cgtcatatct gaaacattca gtacttcctg aataaatttg actgttatcg tcccatagcg
     8101 atcacacatc gctaagatcg aacgctgtcg ttctgttttc agcatattct gtacctccac
     8161 gaccttataa taaaacattt acgaacattt ttcaacacat attgttcgtt tattttcgtt
     8221 tatgcgttga caatgatttt gtaagcggtt atactcaact tgtaaacata taaataaaca
     8281 taaacgaact aataaaggag ctgttttgtg tgaatgaatt ctttttcaaa gacactgtct
     8341 atgtttctga caagcagtcg caggaagaag tgttcgagga agtctatcaa gatctgttaa
     8401 agaaagaatt ggtaaccgaa gattttttgg agaacctttt agaacgggag cgtaattatc
     8461 cgacgggatt agatctctca cctgtctcta aaatattgcc agatatcgct atccctcata
     8521 ccgaaagtga gtttgtgcga accacccgta tcattccaat caaagtaaaa aatgaaatcc
     8581 ctttctacaa catgatctcg ccaagcgatc agatttccgc ttcatttctt ttcatgatct
     8641 tgaacgaaaa tggtgaagca caggctggca tgttggctaa tatcatggat ttcattaatt
     8701 ctgttgacga agaaaaactg ttttcgtttt tcaaattaga ggacacagaa gcgatttatc
     8761 attttttaga agaacatttt tatatataga agtctctgcc taaccgcagg ataaaaaata
     8821 aacttatttt aaggggagaa ttattatgat caaaatttta gcagcatgtg gagcaggcgt
     8881 taattcaagc catcaaatca aaagcgcttt ggaaactgaa ttgaaaaatc gtggctatca
     8941 agtcacctgt gacgccgtta tgatcaaaga tatcaatgaa gaaatgctgt ctcattatga
     9001 cattttcgca caaattgcca acaccgactt gggttttgac attaacatcc ctgtcgttga
     9061 tgcaggtgct attctttatc gtattcctgc tatggctgag cctgtttacc aaagtatgga
     9121 aagcgcaatc caatcattaa acaaatagga ctaaagaaag gaaaagcata tgaatacaat
     9181 tattgatatt gccaataaag tatttacccc cctaattaat ttaggtgcgg caccgttaat
     9241 gaccatcgta ttaactgtta ttgctttatt ttttaaagta aaaccgtcac gcgctcttga
     9301 aggcgggctc aaattaggga tcgccctgac tgggatcgga gctattattg gaattttaac
     9361 tactgctttt tcagacgcta tgacagcttt tgttgaacgc acaggtattt cattaaacat
     9421 tacagatgtc ggttgggccc cactagcaac catcacatgg gggtcgccat acacgcttta
     9481 ttttttacta attatggtga tcgtcaatat tgtcatgtta gcaatgaaaa aaaccaacac
     9541 attagatgtg gatattttcg atatttggca tttagcaatt gttggtttgt tcgcgatctt
     9601 ctgcggtgct aatctgttta ttgcttctgc attagttatt ttcattggtg tattaaaaat
     9661 ttggaactcc gacttaatga aaccaacctt caatgacttg ttaaatgcgc cagacagcaa
     9721 cccgatgacc acgactcata tgaactacat gatgaatccg attattatgg ttttcgataa
     9781 aattttcgac aaaatcttcc cttggttgga taaatatgat ttcgatgctg ccaaattgaa
     9841 tagtaaaatt ggcttctggg gatcaaaatt cgcgattgga atttatttgg ggatttttgt
     9901 tggattacta gcaggacaaa caccaacgca aattttctcg ctagccttta ccgctgccgt
     9961 ctgtttggaa ttgttctctt tgataggcgc ttggttcatc gcagccgtag aaccgttatc
    10021 acaagggatt accgattttg ccaataaacg actaaaagga cgtaccatca acataggttt
    10081 agactggcct ttcatagcag gccgcgctga aatctgggct gccgctaacg tattagcacc
    10141 aatcatgttg ctagaagcca tcatcttacc aggaaataaa ttattgccac taggcggaat
    10201 catcgcaatg ggtgttaccc cagcactatt ggtcgtaact cgtggaaaat taatccgtat
    10261 gatcgtaatc ggcgcgatcg aattaccatt attcttatgg tccggcacct tgatcgcccc
    10321 gttcgttact caaacagcta aagcagtcgg agcctttcca gccggcttga acgggtccgc
    10381 catgatctct catacaacga tggaaggccc gattgaaaaa ttcctaggtt atttagtcgg
    10441 taatgcttct caaggacaaa tcgaatttgt gatatacgct gccttagcat taggtgcata
    10501 ccttttaatc ttcatttggt acgctcgtca aatgaaaaaa cgcaacgctg catacgcagc
    10561 tgaaaaagaa gagaatgcat ctccggcaac ggcgcataat atgacttatg cagccgaagc
    10621 taaataagct aaaaacaccc cgaatgtctt tagacttcgg ggtattattt tacctataaa
    10681 aaataaatag atgtaaaatc catttcaaaa agcatagccc atctcttgcc acacccaata
    10741 aagaaattat caacgtttat gcttttttta actcctttaa gcttgccttt gttggtattc
    10801 ctcaacgatt ctttttgtgt tcttgtttgt tttgtgcttt ttgacttttt gttgtttaat
    10861 tgcgaattcc ttgatttctt ttttcctgaa tcgcttcgca tcccgcggtt atgcgtgcga
    10921 tttcatcatt gctttttctg atctaactcg tttcatgtca aagagcttca gctgacttag
    10981 tgtccatggt tgttttgata tcccttttcc tccttaataa agcggtgatt gatactgcta
    11041 ttttttcctt cacataaaca taacatccca aaaaaatcga ctatcttttt tcaaatggtg
    11101 tattcaagta ttcggaggaa ttagtaatga tgcaccctaa aaataacata attcgcttaa
    11161 attcaaggct accaaaaaac taccaagttc taattatcta gagtttggca gtttctcatt
    11221 tacacaacat atttttcttc tttttcgatt ttttcatagt gcttttgttc atcatcatcc
    11281 gttataacta ctgttacatc attcagatta taatatactg aaaaatctct tttcccaatt
    11341 ttcgaatgat caatcaataa aaagcgttca acagaattat tcagagcgat ctgttgagtt
    11401 tggccctctt caaaagtcga agtcataatt ttctgttcat ttaaagcatt acagctgaaa
    11461 aatgctttat gaaaattcat atcctgtagc gatttattcg tgatttcacc aacaaagcat
    11521 tgagtcgttt cccgcatctc gccacctaat agatagattt ttcgattccc ttttcgctta
    11581 tttaattctt caaaaacagg caaacagttt gtaacaatcc gcagtgaatc cccttgaatg
    11641 atttttgaca tgagctcgat tgtagttcct ggtccaagaa atatcgtatc accatcattg
    11701 atcatttcat tcgccttttc cacaatccgc tgcttttctt cttgcagcat aatctgtttt
    11761 tctctatgag aaagctccac atgccgataa aaatccttca atttcgctcc gccatggacg
    11821 cgttctaata atccttgttt ctccatttcg tccaaatcac gtcggactgt catatccgaa
    11881 accgaaaact tctccataat atcgctgact cgtactgtac cgttaacctc taataacgac
    11941 atgatcgctt ggaatcgatc cgtttttaac atttcatcgc ctcttttctt tttaagacta
    12001 tcaaattctt aagaaaagtg ctagaacaag ttttcttaag aagccttctg caggaaggaa
    12061 agcaaaattt ttcagaattt gcaagctgct attctaaaaa ataaaaccga acgagcatga
    12121 ctgtcatagt gacagccttg ttcattcgac ttttatcttt aataaatcat tttatttaat
    12181 ctgaagttat tgtgcaagtt caaaaacaaa gtagttaatg tctcctcgat atttcgaaat
    12241 cgaatactca acgggcagac gttctagcga aaaaccctgt gtatggaaat agaacattgg
    12301 atctccttct tccacatcta acaaatcgct gatcgtatca tccgctttga ttacttctag
    12361 ttttctggtg accgttttaa ttggataacc catttcttca aaaacttgat acattttttc
    12421 ttttctgatt tgtcaaatta agctagacaa tatgtcttta tctatgcagc tcaaaaatac
    12481 ttccaatggt gttcgataat ttaatgattt tcttggaata tggtttcttt tcgaggcaac
    12541 ggattgaata aaggtttcat ccatgtccct aaagtccatt gttttaggta agccatctcg
    12601 tctaagcaag ccgtttgagt gctcatttag ccctctttgc gaaggtgttc ctggatctgc
    12661 gaaaaagatg gcaatatcgt tcgagttact tatagatcgc cagttggaaa attcttttcc
    12721 acaatcaaat gtcatggatt taaacaagtg tttaggcatg gatttgaacc aatcatttaa
    12781 cctgttttcg atatcttcgg ctcgtctgcc tgtcggtttc aaagtaataa taaccttgga
    12841 taaacactca accaatgtaa taacagcgct cttgtgttta actcccacaa tggtgtcgcc
    12901 ttcgagatga ccaaattctt tagtcaggtg tggatagaga ctgttccgtt catgaatcgt
    12961 tctgcgaaag gcctgttttc cacgcttttc ttgatgatta ttcggtttac gtttcccctt
    13021 cataggtaac gactgtgtgt caaaaaggcc ttttttaaac atccgataga gtgtacgaac
    13081 agaacaatca attggaaact cgccacggcc gataatcaca tcgggtgacc acccttgagc
    13141 taccttttct tgaacatatg ttgattgttc atcagataaa acggttgaac gtcgtccaca
    13201 acgagattta ttttgtttgt attgacggta ataatctagc acttttttcc ttgtttgaga
    13261 aacgaataaa cattataaat ggtttgtcta gagcgattta aactttgtgc gacaagagcg
    13321 acaggtttct cgatattgaa ataagactct atgattacta gttcgtctgt tgtaagatgg
    13381 gtgtaggtca tttgtactca ctccttatta attctttggt cggaactatt ttgagtgtag
    13441 cacaaatgat ctttttagtt gtctagctta attttacaat cgacgcctca aaaagaatac
    13501 ccgaaattta aacagcaaaa aaagatagcg cttaccattg tgtttaagat aaaacgctag
    13561 ctaagttggc tagcgcttta ttaatctaag caagctctaa aattgcttca atacaaaatc
    13621 aactgctcct ttaggatcac gtgttaaatt gatatattct gcacctttag ttgcaattac
    13681 ttttatccct aatttgtctg catctttacg gagatcatcc aaataggaat tcacttgagg
    13741 tgctagaacg attaaatcaa aatttttcat catatcataa tgagaaccat aagctcctgc
    13801 tttagcagaa agattaacac ctggttctgt tgcgaagttt gaaaatcaaa cgcgccctct
    13861 ctgaactcca aatatcttag gctgtta
//
LOCUS       DACDKJ010000076        13852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDKJ010000076 DACDKJ010000000
VERSION     DACDKJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.076"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IV328_002443"
     CDS             complement(<1..902)
                     /locus_tag="IV328_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8938543.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IV328_002444"
     CDS             1185..2153
                     /locus_tag="IV328_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8938544.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IV328_002445"
     CDS             2266..2544
                     /locus_tag="IV328_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8938545.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IV328_002446"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IV328_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8938546.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IV328_002447"
     CDS             3010..3756
                     /locus_tag="IV328_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8938547.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IV328_002448"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IV328_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8938548.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IV328_002449"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IV328_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8938549.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IV328_002450"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IV328_002450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8938550.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IV328_002451"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IV328_002451"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8938551.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IV328_002452"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IV328_002452"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8938552.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IV328_002453"
     CDS             8493..9035
                     /locus_tag="IV328_002453"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8938553.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IV328_002454"
     CDS             9058..10614
                     /locus_tag="IV328_002454"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8938554.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IV328_002455"
     CDS             10628..11530
                     /locus_tag="IV328_002455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8938555.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IV328_002456"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IV328_002456"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8938556.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IV328_002457"
     CDS             12973..13395
                     /locus_tag="IV328_002457"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8938557.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4575 a         2474 c         3013 g         3790 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag acttttgtgg ctctttgtca ataaggactg atgagttgtg
    13741 caaaatcaaa tctgagtcag aatgaaccac attctggctc agattttttg ttatcttaca
    13801 ttgattaatt gattgatcaa aaagattcta attctcatgt tctcaaatga tt
//
LOCUS       DACDKJ010000077        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDKJ010000077 DACDKJ010000000
VERSION     DACDKJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.077"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IV328_002458"
     CDS             complement(<1..914)
                     /locus_tag="IV328_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8938558.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IV328_002459"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IV328_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8938559.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IV328_002460"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IV328_002460"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8938560.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IV328_002461"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IV328_002461"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8938561.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IV328_002462"
     CDS             4440..5321
                     /locus_tag="IV328_002462"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8938562.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IV328_002463"
     CDS             5318..6034
                     /locus_tag="IV328_002463"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8938563.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IV328_002464"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IV328_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8938564.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IV328_002465"
     CDS             6751..7752
                     /locus_tag="IV328_002465"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8938565.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IV328_002466"
     CDS             7749..9110
                     /locus_tag="IV328_002466"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8938566.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IV328_002467"
     CDS             9070..10221
                     /locus_tag="IV328_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8938567.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IV328_002468"
     CDS             10196..11365
                     /locus_tag="IV328_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8938568.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IV328_002469"
     CDS             11540..12919
                     /locus_tag="IV328_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8938569.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IV328_002470"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IV328_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDKJ010000078        13398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDKJ010000078 DACDKJ010000000
VERSION     DACDKJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.078"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IV328_002471"
     CDS             complement(14..706)
                     /locus_tag="IV328_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8938570.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IV328_002472"
                     /pseudo
     CDS             791..967
                     /locus_tag="IV328_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IV328_002473"
     CDS             complement(1143..1712)
                     /locus_tag="IV328_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8938571.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IV328_002474"
     CDS             complement(1786..2109)
                     /locus_tag="IV328_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938572.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IV328_002475"
     CDS             complement(2263..3075)
                     /locus_tag="IV328_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938573.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IV328_002476"
     CDS             3429..4277
                     /locus_tag="IV328_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8938574.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IV328_002477"
     CDS             4422..5363
                     /locus_tag="IV328_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8938575.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IV328_002478"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IV328_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IV328_002479"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IV328_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8938576.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IV328_002480"
     CDS             complement(9827..12013)
                     /locus_tag="IV328_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8938577.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IV328_002481"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IV328_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8938578.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IV328_002482"
     CDS             complement(12235..12675)
                     /locus_tag="IV328_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8938579.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IV328_002483"
     CDS             complement(12749..13288)
                     /locus_tag="IV328_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8938580.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DACDKJ010000079        13046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDKJ010000079 DACDKJ010000000
VERSION     DACDKJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.080"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            178..384
                     /locus_tag="IV328_002484"
     CDS             178..384
                     /locus_tag="IV328_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8938581.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(586..1587)
                     /locus_tag="IV328_002485"
     CDS             complement(586..1587)
                     /locus_tag="IV328_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8938582.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1592..3505)
                     /locus_tag="IV328_002486"
     CDS             complement(1592..3505)
                     /locus_tag="IV328_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8938583.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3673..4179)
                     /locus_tag="IV328_002487"
     CDS             complement(3673..4179)
                     /locus_tag="IV328_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8938584.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4339..4779
                     /locus_tag="IV328_002488"
     CDS             4339..4779
                     /locus_tag="IV328_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8938585.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            4938..6278
                     /locus_tag="IV328_002489"
     CDS             4938..6278
                     /locus_tag="IV328_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8938586.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6347..7504)
                     /locus_tag="IV328_002490"
     CDS             complement(6347..7504)
                     /locus_tag="IV328_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938587.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7507..7863)
                     /locus_tag="IV328_002491"
     CDS             complement(7507..7863)
                     /locus_tag="IV328_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938588.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8161..9135)
                     /gene="sgrA"
                     /locus_tag="IV328_002492"
     CDS             complement(8161..9135)
                     /gene="sgrA"
                     /locus_tag="IV328_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8938589.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9331..10170)
                     /locus_tag="IV328_002493"
     CDS             complement(9331..10170)
                     /locus_tag="IV328_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8938590.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10355..11242
                     /locus_tag="IV328_002494"
     CDS             10355..11242
                     /locus_tag="IV328_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8938591.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(11826..12521)
                     /locus_tag="IV328_002495"
     CDS             complement(11826..12521)
                     /locus_tag="IV328_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8938592.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12505..12903)
                     /locus_tag="IV328_002496"
     CDS             complement(12505..12903)
                     /locus_tag="IV328_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8938593.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3911 a         2640 c         2022 g         4473 t
ORIGIN      
        1 agacgtatga tggtaaactt tgtacctatc tgattgctga atcattaatt gcttatcatt
       61 agccatcttt gtttcaagtt cgctagatga tcattttatt cacatcctaa caaacttgtc
      121 acatttatag attcttaatc tatttatcat acaattataa aaaaggaggt gctgcttatg
      181 aaagctatcc atatttcttt gctttttttc gctagtgcat tgattttatt tgctattcat
      241 ctttgttccc agccttatat caataacaac ggaatattag tggaaccagc attcttttgc
      301 ttaccactcg gctttttgtc ccttcttgcc tccgcaggct tcggatttgt tgcttttttt
      361 aaacgatcaa aacaacaaat ctgacttata aaatttacgt aaaagagatc gtgacaataa
      421 tcgtgtgata ttcccaaatt tcgaataaaa ggtgcccctt gccatctcta actcctcagt
      481 cacaagttac aatattcata aaccatgaaa agcttgttta ttgaaattgc tccttgtgac
      541 ttggagctaa gtgacagatt acaacctctt ttgcatcata agactttact ccttgaaaaa
      601 cattactaac ttttgatatg tttcttgcgc ttgtttattg ctgaaatcaa actgatattc
      661 atggttcaaa acagcatttt tctccgcata atctactttt gtgactttca cccccgcttt
      721 ctcaaataca gaagttactt cttccatttg tttcgtaaac gtatttgtat ttccatccgt
      781 taagaatgtg ttttgcgtcc aacgatccgc atgtctgacc aaggctaaac ttttgacctt
      841 ctctgattgt tcaaagtcct tattatcaaa gtaagcccat ccacattttt cataaagaaa
      901 gctagagatt ttatcactta cttgagtaag tgcagaagga tccaacagac cagacaaaga
      961 aataaacttc gtaatctgct tgttctccaa agtcggatca agatgatcag cttttttaat
     1021 ttcttcattt tgaagtgcta acaaatattc tccactaatt tgtgcacctg ccgaatttcc
     1081 accaatcgaa atattcgacc aatctgcttg atcttttatc tcttttttag ctgcttgaat
     1141 ggcttcgttc aattggataa ctggtgtagg atgttttgct tccggtgatc gttcataatc
     1201 tatatttacg atgatttgct gcgtgtctga acatagatat tccaaataat cacgacaatc
     1261 atttttatcc cctcctacat atgcgcctcc atgcatccag aaaagaatcg gtaatttttt
     1321 cttttccact ttcggtgtgt agatatccat attattattt ttgtactttg aaggatagga
     1381 tatttctttt tttatatcga tttgttcgat tttttgctgg aaatcatctg gtcgggcata
     1441 tacttgttct ttttcacttg tgtcaaattg ttttctaaca agcaagctaa tcggctttgg
     1501 tgtgatttga ttaatcaaaa atacagtgac taaaataact acgatgataa ctggtactgc
     1561 aatcactatt cgcttgaacc atttcattca tttaccctct ctttcttcgc caaatccatg
     1621 tgataatcgt gctaacagat aaaaatccac ctgaataaat gagaagcgaa tgtttctttt
     1681 ctccagtttt cggcaacatt cttttttttg catctgtctt tttttgtact ttcactgtca
     1741 tctgaatcgg ttgcgtatca tcacttgatt gatcctctcg aggttcattc tttcccttat
     1801 tgtctgcatt ctctgtcgga tcttgctttt ttggtggagc tactgggatt tttttgtaat
     1861 agaaaataat agttggtatc gtctcattaa aaatcccttc agtcggtcct tcaacatttg
     1921 ctaattcgta tccttcgaat gtcttttgct ctgtcatata attttctcca taagctccgt
     1981 aaagctggat ttcgtcctgg tctttgagta gacttttatt gttttcatcc acatacttta
     2041 cgtttatctg atcatggaac actactttca aaaagtccgt tacatgtttc gtgtaggcag
     2101 catggtcaac ttcctgtgcc tttccatgcc ctgctccttc tactaataaa agatctttat
     2161 accctcctga taactcatat aatttttgcg cgttagacac aggtactaca tcatctgctg
     2221 ttccatggat aatcaattta ggcaatgaat ctgacattcc aatcgtaggt agagcagctt
     2281 ttgtatccaa atgcatctcc tgatcaaaat agtattgttt caaaaggccc ataaataaat
     2341 cttcatgctc ttgatcccca atgatgtcta actgattttt tatggcatcc ggtagtgcgg
     2401 gggctactgt ctggctgtac gtaaaacgta gttgttcatc aattgatgca aatccgcaat
     2461 tttcaataat tcctttgaca ttgacaggaa tatctttcgc taatacagac atcatcgtag
     2521 ctgctcccat cgaaccacca taaagtaaaa tcttttgaga tggccgctct cgaacttctt
     2581 gatttatcca gtaaaggacg tcatctgact catattgtcc ataggtcaca taatcgcctc
     2641 cgctattacc agtcgctctg gaatcaacaa ataataaatt atacccggcc ttataaaaat
     2701 cattgtattc ttctgtaacg atcccgttat tccaattccc tcgaaatccg ccatggatca
     2761 tgactgtttt atctgaacct tgatcgacat aatatgcata tacatccatc attttgccat
     2821 cttgtttgtt tagtacagtt tgatgaagtt gttttgtatt tggatagaag cctgtgtttt
     2881 ctgtaggcgt tgtcccatca tctgtacctc caagcatcga catcaacaca ctgttttttg
     2941 caaaagcgat atcgtacaat agataagtag caatcagatg tttgataatc tctttatcta
     3001 caccataatc attaacagca agctcagtga ctaataactc tattaatttt gacttgatgt
     3061 cttctgttgg tccattaagt atttctgtca tctgtttacc gaagtaattc aaatattctt
     3121 taccattgaa attttcacct ttaccgatat catcattttc tgtcccattc tctttgtcac
     3181 ttactttagc agaagaggat tcgtcgtttg acatttttgt agctgttgct tcttctgttt
     3241 gttggttatc tgtggtagcg gtcgtttctt gatctgtgct agtaacagat gtctcaaccg
     3301 ttgtttcagt atttgtttct gtttgtgaag tactcgattt cgtttcattt ccagcatcag
     3361 tagtttctgt ggtttctacc tcccgagatt ccgattcgga agaaatgctc tctccatata
     3421 ctgtccattg cgggacgctt gtacttacag caaataagat gaccaacgct ccctttaata
     3481 cttgttttgt tctcaagcta ttcactcctt tttatgtttc taggagaatt atatgtaaac
     3541 gcaaacattt tttatacaaa aaataaatgg tttatcccat aaaaatagaa caggattatt
     3601 ttctcatttt acgtaataaa gcagaatgga taaaccagta tccattctgc tttttttaaa
     3661 cgaagaaaca acttattgct ccaaaaatgt cagaaatcgt ttatcccaaa ttttctcgta
     3721 aaaagcgatt gtttcctttg ctttcataaa aggattatca taagttgtcg ttgctcctct
     3781 acacatcata agctggtatc ctaatccatg agcaaacttc acagtcgtat ctatacaata
     3841 ctctgcttgt gccccacata attcgatcga atgacagtcc aattcagtta ataaaacact
     3901 caaattagtc ttataaaatg aattcgcatg ggtttttctc acaaaataac tgtgctcagg
     3961 aatagataat tccgacaata gctcccatgg ttttgtttgt ggcactaatt gagcatcttc
     4021 gtgttgaacg aaaataactg gtttcccaat cgtttcgtat gttttgattc ttttattcac
     4081 taatgctgtc actgtttcta attcgtgtat cggcgaagaa tcaaaacata cccctttttg
     4141 taaatcgatc actactattg cgtcggctgt gtttgacata cttgtgcctt ctttcttttt
     4201 tcttgattta atagtatttc ttatctagct agttccatta tacgtgacaa cattttctta
     4261 ttttacacct ctaaatggca cttttcgact atttcttcat gttattattt cataaatact
     4321 tagctattgg aggaatgtat gatgattaaa ttgtatacag gttctggctg ctcgtcttca
     4381 agacgcgcaa aacgttggct tatcgtccat gatataccgt ttatcgaaca gaacttaatt
     4441 cgtcaaccac taaccaaaaa tgaatttatc cacttactaa aattgacaaa caacggcgtg
     4501 accgaaatgc tctctactca ttctattagt tataaaaatc ttgttcacag aatagatgaa
     4561 ctcagtttga gtgaactata tattgagatt cgtacatccc cttctttatt gaaaaaaccg
     4621 attatctacg accaatctgc aaaacttttg actggattta gcgaagataa aatcaggatg
     4681 ttcctttcta aaactaaaag aaagcaagag ctcaacgaac tcttgctttc tggtcatctt
     4741 gtcgatttta aagaaaataa tctttttgca atgtcttaat tttggcataa acaatctgct
     4801 tttctggatc tataatatct agtgttggtg acgccttctg ttgccaatac tttttctttt
     4861 ttctaataaa cgcaaacaga caccttgaaa tcctttagaa taaagtcgac gaaaaccatt
     4921 caaaggagtg tttcaagatg tcctattcag agtttaccaa agagttacta gatattctag
     4981 acctaaatct tacctttcat gaagatgcct ttcgcaaaga gcgcattaat gacgaaacat
     5041 gttttgtttt tgatggcaca ttgacctatc aaccagagga atgcttccat tgccactatc
     5101 aaaataagca aacgatcatt aaatggggct ggaaaaaagt atccatctta ttgaatgatg
     5161 tcagtaacta caagaccatt gtgcggatta acaaacagcg cttcaaatgt aaacactgtg
     5221 gaaagacttt tttagcagaa gattcagtta gcgatcgtcg ctgctccatt gcccgcagag
     5281 tcaaacaagc cattcttgag ctattaagtg agcctatttc tatgtctttg attgcacgta
     5341 tgaagcacat ttctccgacc actgtcattc gaatacttcg cagccttcgg cctaaaaccg
     5401 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
     5461 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
     5521 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctagaaaag
     5581 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
     5641 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
     5701 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
     5761 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaatg
     5821 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagcg gagatagtcg
     5881 atcggctatt atattatgat gactccttaa agcgaggcta cgaagtctac caggtttttc
     5941 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
     6001 aggagttgcc agagcactat caaccagtgt tcacgacctt caagaaatat cgaacagaaa
     6061 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
     6121 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac cgagaacgaa
     6181 tctttttata tcgaggaaaa tatttcaaga aaactaagaa cactacccaa ctgactaaag
     6241 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
     6301 ctattgaatt tcttcaccaa cactcattga cgaagagccg cttttcttaa ttaacgaaat
     6361 ccaatttttc caatatggat ctatttttct cattcctgat ttttatgatt tcagctttca
     6421 gacgcgccca atcatttttt gtaggttgtc cattccagta aaatatctgt gtattcgttt
     6481 gatcatatgc ctttttgtcg ataggataat cgctaatgac aatatcaggt aactgatcag
     6541 cggatgcact atattttatt gggcgttcag tcatagataa tatccttttc tttgcccata
     6601 attcctgtgt tttaccatac tttgattgaa tatagaatat gacaggttgt tcgtgtttta
     6661 taaaaatatc cctgactaac atacagtatt gaaaaatcaa ccgtctgttc ttttcataat
     6721 aagtattaag ctctggaatt ctgcgagcta ccccactaaa aaactgttgc atgattggat
     6781 acatcactgg aaatatatgc tgaaaatctc tgtcttctgc tgattttcca aaagcatcca
     6841 ccttcttttt tgtcccaaaa acccttgaat acaaatggat cattgccaaa ttgacatata
     6901 gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt tttttcaaaa ttttctacaa
     6961 tagacgattc aatccagccc caactttctt gtactttatc tggagtatcc aacaaatctt
     7021 cttccaaata ggtgttcatt acaccaaaca taaaataaag gtaagcttgc tctgttggag
     7081 tgatgtgatt catcgaccag aagtttgctt caaaaaaagg ctctagtatg taatgcgtaa
     7141 actcttcttt tgttatcaat ggatgcacaa atttgttcat ctcttctggg acatacgtaa
     7201 tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag taggctgatc catttctttc
     7261 tatctatact acgcgtatat ggcgcatgtt tcgcaaattt ttctaatagt tgacgtttat
     7321 tttttatact tgataatata tcagaatgat tttcgtcgaa tgactgccaa tacatcaagt
     7381 gaaaaaagta tcggatacgg tattcttctc cttctatcgg attacctttt ttcaagctgc
     7441 aagataaatg atagtttttc aaaatagtat taagttctct taattttctt tgaatcgtac
     7501 gcaaacttat ataatttttt gactaagatt ttctaaggat tgataagtac ctttgtaaag
     7561 tgaatccagt aaaatataac caatcgttcc ctctaaaagt atagaataaa ctttactcag
     7621 taaaaactga gaagaaaact cgacttttat catcttgttt tctgaaagaa aagataattt
     7681 atattctgga tacctttcgc aaaacgattc tgcttgttga atcaccgaat tcatcttagc
     7741 cagggtgtag tttagatcta aagcgatatc cttcaccaca tataatttct cgtctctttc
     7801 ataacaatag ttcaaaaatt gatatacttg cctttcattg atatcaataa attcttcaaa
     7861 caaagtacat actccttttt atctattatt tagacttatt ccgacagcta catgaataac
     7921 gtacaaatag attcgttact gatttttcag agatactacc atgcgcagtg tgcattgtca
     7981 attattcttg ctcttatttt ccatttgtgt tcctgttatt tcctgtttct caaatatgtt
     8041 agtaaacaaa aatagatctg gcaaagctac aacttcgcca ggcaatacag cactctcgaa
     8101 tgaaatattc actcaaaaat ctatcgtttt attcaatttt agatctgttg cttttattct
     8161 ttatttcact cgatggaaga gagaacgaaa atagcttgct cctacaatca aaatgagtaa
     8221 cacacccatc catgtaatta gcttgttcaa ggttctacta ccagtttctg gtaaatcgct
     8281 ttttctatta tctgtttctt tcgctttttc ttgatgatca attccacgag aaacaaaatc
     8341 aggctgtttt agcacacttt ttaggttgct ccctgcattt ctcttttgtt ccttagttgg
     8401 tatgataggt accaaatcaa gttcattatt tacttttcct gtgctgcttt cgctgctcgt
     8461 gcttcctgtg ctactctcac tactcatgct tcctgtgcta ctttcactac tcgtactctc
     8521 cgtactgctt tcgctgctcg tgcttcctgt gctactttca ctactcgtac tctccgtact
     8581 gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc gtactctctg tactgctttc
     8641 accgctcgtg ttttctgtac tgctttcact acttgtgcta tccgtactgc ttggcggtcc
     8701 aacaactgtc caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc catcataaaa
     8761 aacaatcaaa tcaactttat ctttactctt gatctcatca atcgatagtt ctgcagaaat
     8821 cattattgta tactcaatac cagcggtcca ttgtacgtct tttaaataaa cttccccaat
     8881 aactttttct gaacttccat taccatccca accatatgtt agaacagcat gtacatcact
     8941 agcattcacg cttttgtttg gtttgactgg aatagtaatg acttgttgtg tcatgtcata
     9001 aacaatccga tcagttttca cagtaaatga aagattctca tttgcccgtt catttgcaga
     9061 agtactgatt ccaatcattg ctgcaattat actcagccca agaataacaa ataatacggt
     9121 cgctgttttc ttcatttcta tcactctcca tataattacc ttctatttga taaaggaatt
     9181 atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac aaaagatgtc
     9241 gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt aaaaaagcaa
     9301 gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt atttcttcat
     9361 ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca agaataatgt
     9421 agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat tttgcgattg
     9481 gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac ccaaaatagc
     9541 tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac ttcctgtact
     9601 gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg ccagaaatgt
     9661 catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa tcattgctat
     9721 taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa gctcttctga
     9781 tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt ttgcgtttcc
     9841 tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca gaaacactcc
     9901 aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa cagaaacacc
     9961 ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta gtaaaaaaac
    10021 agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc caataaacat
    10081 atccgcaagt gcattattga caagaattcc taaaacaaaa ccaactaaga ctattaaaac
    10141 agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat taagtttaac
    10201 aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat agagagaata
    10261 gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac tgtctcccta
    10321 attcttctgt tagactgttt ttaataagga aaggatggga aattttttga aaaaacaact
    10381 actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta gcaagtccaa
    10441 tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt atgataaccc
    10501 aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca aagcatttga
    10561 tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata atgaacaaat
    10621 caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa cagaagagac
    10681 atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag ccaatgtaaa
    10741 attaacagat aaagatctag acactgcttg gaaagaattc tatccagaag ttagtacaca
    10801 gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag caaatgaagg
    10861 ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa aggaaactga
    10921 tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa aaaaagcagc
    10981 atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc caacgactta
    11041 tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt ctaataaaga
    11101 caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg atacggaatt
    11161 catggataaa acaatccgta aagtgatgaa aaaagacaat gtaactatta aagatcctta
    11221 tgtaaaaaat atattcaaat aaacaaattt gacccaattg gggatgtggc aaaatttgag
    11281 aagtgatttt tggaaattcc ttcttatttt tgaggcaaac cacttttgcc acaacttcat
    11341 cggggccttt ttttatttgt accaaaacaa atcactggat ttgttacaat catgaggata
    11401 taatttaaaa aagtttttta tttctatcac atgaatcaat gcgccattaa gtagattctt
    11461 tcatgcttcc ctattttttc acttgccgta tcgcttcaaa aacaactatc ataaactcaa
    11521 actcgactga aaagttattt taaaaaagac taaaaatatc ccttctggac ttgttcctct
    11581 tattctgtta tactagagaa acacgaaaga tatttttatg cgacactttt catttgtttt
    11641 tatgaaattt attcaaaact ggcgtaactt cagagttaca aggatgtatt agaggttagg
    11701 gaatacatcg tttaaatgag aaataagtaa tagtcaccca aactttaaat taatgaatat
    11761 tgaatcgtca gatagcctcc tcgagttttc gagaaggcta tcgtttattt tatggagaaa
    11821 ttgattcaga agtaatcagc gacctttttt agttcaccaa tcgtttctgc ttggaaagtt
    11881 tgcacccaat cttcttggaa gcccctcact gctttatcaa tcgagggcat ctctaatact
    11941 tgatctagta gctctttacc taacgttatc gcatgagctc cagatcgaat ggctgtagtg
    12001 acttgttcac aatttttgaa actagctgct aatatttttg ttggtctttg cgtacgttcg
    12061 atctcagtcc gcaacgaatc aatcactttc atagcatcaa tgccttgatt ttgcatcctg
    12121 ttataataag gtgcaatata atctgctcca ttattaatag ctaaataccc ttgtaattct
    12181 gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt tcaacttttt gatcgcttga
    12241 aggcctgctt gattcaccgg caccttgata aacgtatctt ttcccagttc ttctatgata
    12301 actcgagcat cctctatcat ttcttcttcc gttcttccta ttgtctgaat atggatagga
    12361 atctgtccga ttattttttt gatttctttc atatgcgaga agaagtttga cgctccttct
    12421 tttttcagaa ttgtaggatt tgttgttacg cctgttaaat tcaaaaactg ttgacaagaa
    12481 taaatacttt ttgtatttgc tgtatcaagc atgatttcca tatagctttc ccccattctg
    12541 atcctcttga tcgattttaa tgattgtccc ctttcttaaa tgcggcattt ccttatcttc
    12601 tacatacatt gttcctggaa gttccggctc atgagatcca tcaaatttta tcgtgatatg
    12661 tcctaattca ttcaaattag tttggacaac atctcctact gctgtgatta catacatttg
    12721 tttatcaaaa cagagcttca tatcacaagt tatttctttt cttacctgat tcaattcaat
    12781 cttataacaa taatctgtta atgtttccgg tgcttcatct ccaaataaaa tcattatttt
    12841 ttctttcctg aatacttctg cttctatacc aacatctgtt acaaccgttt caaagatcgt
    12901 catctctgtt cctcctcgta aaatatcatt tgtctaatag tttttattca aaatgtttct
    12961 agattataaa atttattgaa taacacattc ctaacggaat ttgtttcgat attgtgtgat
    13021 ttctcagagt acaatggatg tatgga
//
LOCUS       DACDKJ010000080        12736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDKJ010000080 DACDKJ010000000
VERSION     DACDKJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.081"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="IV328_002497"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="IV328_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8938594.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="IV328_002498"
     CDS             complement(324..893)
                     /locus_tag="IV328_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8938595.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="IV328_002499"
     CDS             1079..1981
                     /locus_tag="IV328_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8938596.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="IV328_002500"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="IV328_002500"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8938597.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="IV328_002501"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="IV328_002501"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8938598.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="IV328_002502"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="IV328_002502"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8938599.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="IV328_002503"
     CDS             8549..8734
                     /locus_tag="IV328_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8938600.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="IV328_002504"
     CDS             complement(8796..9992)
                     /locus_tag="IV328_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8938601.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="IV328_002505"
     CDS             complement(10162..11598)
                     /locus_tag="IV328_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8938602.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="IV328_002506"
     CDS             11891..12700
                     /locus_tag="IV328_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8938603.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2249 g         4008 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctagtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc acgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaacgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgaca
//
LOCUS       DACDKJ010000081        11966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDKJ010000081 DACDKJ010000000
VERSION     DACDKJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.082"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(211..951)
                     /gene="truA"
                     /locus_tag="IV328_002507"
     CDS             complement(211..951)
                     /gene="truA"
                     /locus_tag="IV328_002507"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8938604.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1142..1777)
                     /locus_tag="IV328_002508"
     CDS             complement(1142..1777)
                     /locus_tag="IV328_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8938605.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(1924..3165)
                     /gene="sstT"
                     /locus_tag="IV328_002509"
     CDS             complement(1924..3165)
                     /gene="sstT"
                     /locus_tag="IV328_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8938606.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3363..4361
                     /locus_tag="IV328_002510"
     CDS             3363..4361
                     /locus_tag="IV328_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938607.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4374..6392
                     /locus_tag="IV328_002511"
     CDS             4374..6392
                     /locus_tag="IV328_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8938608.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6452..7168)
                     /locus_tag="IV328_002512"
     CDS             complement(6452..7168)
                     /locus_tag="IV328_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8938609.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7395..7937)
                     /locus_tag="IV328_002513"
     CDS             complement(7395..7937)
                     /locus_tag="IV328_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8938610.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7942..8169)
                     /locus_tag="IV328_002514"
     CDS             complement(7942..8169)
                     /locus_tag="IV328_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8938611.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8248..10128)
                     /locus_tag="IV328_002515"
     CDS             complement(8248..10128)
                     /locus_tag="IV328_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8938612.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10317..10931)
                     /locus_tag="IV328_002516"
     CDS             complement(10317..10931)
                     /locus_tag="IV328_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8938613.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11073..11633
                     /locus_tag="IV328_002517"
     CDS             11073..11633
                     /locus_tag="IV328_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8938614.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         3744 a         2382 c         1916 g         3924 t
ORIGIN      
        1 ttactttatc gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct
       61 cactagattt ttatttaaga aagacttttt agctgtacac ataaacaggc taaaaagtct
      121 ttttttatat ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa
      181 acatgcgttt catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag
      241 accttgtgca gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc
      301 gattgtttct atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg
      361 caccatttta tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg
      421 ttcgatagat aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga
      481 aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat
      541 atccaactct tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt
      601 gttccaaaca taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc
      661 aggcatttct tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc
      721 gatcatttct gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc
      781 atgggtgcca gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg
      841 atgcaatact ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca
      901 tcctgaatat ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg
      961 gctcctctgt cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata
     1021 gctccttgct tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga
     1081 aaaacaaaaa acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt
     1141 tttactgttt gagatgcaag atactcaacc tggcaatggg cacataatta aagaaaaaac
     1201 tgattctttg gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc
     1261 ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt
     1321 ggcttacgtc catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata
     1381 aaaaaccact gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg
     1441 aagattcatg ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga
     1501 acgcgatggc tgctgtaact agggtagcta acacacgaga tgtactaaca aatgcagcat
     1561 acaatgcagg caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg
     1621 cttttcttac aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta
     1681 attcaggata atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata
     1741 ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac
     1801 ctatgctttt aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta
     1861 cacgaacatc tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa
     1921 cgttcaacgt tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct
     1981 tttacgcgcg cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc
     2041 agtttccatt gaatcctgga taacaccgat aataaatcca ataccgacga tttgcatcgc
     2101 gacatcatta gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc
     2161 cgcaacacct gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa
     2221 aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt
     2281 aatcgcggca ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc
     2341 ttttgtcaat ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact
     2401 tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg
     2461 attttcttta ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat
     2521 cgtaccgatc aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt
     2581 agcaattgat tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg
     2641 tacgacttgt gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg
     2701 tgctcgaaaa gctagtccca acaatacagc ccagaataag acgccaaggt aattcgcatc
     2761 aataagagat tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc
     2821 caaactttgc ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat
     2881 tgaacttaca ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa
     2941 aataggcttc acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa
     3001 gaaaaaaacc agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat
     3061 tgaaataaat gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac
     3121 aacaatacgt tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca
     3181 caattttaac aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa
     3241 attctaacaa tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg
     3301 ttttttgaga tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc
     3361 ttatgttttt gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga
     3421 gttatggctt cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga
     3481 tttcagcagt cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat
     3541 ttttacaaac ttccgttgtt attggtgcaa atttgatccc actttacatg ggttaccaat
     3601 atcaaaaaat cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc
     3661 taatagccac actagtttgc aatatctttt tttacgtaca agctggcagc ttgaatattc
     3721 gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt
     3781 gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa
     3841 caacaattaa atatctacta ttatttttaa caattggatt agttggctgc tctactctat
     3901 ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat
     3961 ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc
     4021 ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt
     4081 cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct
     4141 tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag
     4201 gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta
     4261 atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag
     4321 ggaaggcatg gctgataaat atcgggcagt tttttatttg atatttatta gcagtgtccc
     4381 tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat
     4441 acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc
     4501 tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc
     4561 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
     4621 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
     4681 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
     4741 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
     4801 cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct
     4861 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
     4921 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
     4981 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt
     5041 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
     5101 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
     5161 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag
     5221 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
     5281 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
     5341 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
     5401 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     5461 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     5521 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     5581 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     5641 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     5701 aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca
     5761 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     5821 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     5881 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     5941 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     6001 atggattaga acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac
     6061 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     6121 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     6181 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     6241 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     6301 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     6361 caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata
     6421 aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat
     6481 taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat
     6541 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
     6601 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
     6661 agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
     6721 ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac
     6781 tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa
     6841 atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc
     6901 tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt
     6961 cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag
     7021 ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt
     7081 ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac
     7141 caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct
     7201 ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt
     7261 caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc
     7321 ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga
     7381 agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt
     7441 ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc
     7501 aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag
     7561 acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat
     7621 ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga
     7681 acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga
     7741 tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat
     7801 tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg
     7861 gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt
     7921 tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc
     7981 taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc
     8041 tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac
     8101 cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat
     8161 ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc
     8221 cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa
     8281 atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat
     8341 cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc
     8401 aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc
     8461 atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac
     8521 atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt
     8581 gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg
     8641 aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac
     8701 ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg
     8761 tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat
     8821 ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg
     8881 atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt
     8941 gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt
     9001 ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat
     9061 cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct
     9121 gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa
     9181 tgtaccagtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc
     9241 gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg
     9301 cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa
     9361 atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg
     9421 agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa
     9481 ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta
     9541 gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg
     9601 tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc
     9661 cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat
     9721 ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc
     9781 ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc
     9841 aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc
     9901 aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata
     9961 cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag caatgttata
    10021 gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa
    10081 ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct
    10141 tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac
    10201 tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta
    10261 gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat
    10321 tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc
    10381 ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag
    10441 ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc
    10501 aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct
    10561 ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa
    10621 gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt
    10681 tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct
    10741 gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac
    10801 aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc
    10861 attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa
    10921 gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata
    10981 gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt
    11041 tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa
    11101 aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag
    11161 caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac
    11221 attataagga tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa
    11281 atgaaattct ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg
    11341 actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt
    11401 cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg
    11461 aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag
    11521 gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat
    11581 tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa
    11641 catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta
    11701 gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa
    11761 gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt
    11821 cacctcatca ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg
    11881 taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta
    11941 cctcaattca ttttcaaggg gaatca
//
LOCUS       DACDKJ010000082        11668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDKJ010000082 DACDKJ010000000
VERSION     DACDKJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.083"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IV328_002518"
     CDS             complement(37..216)
                     /locus_tag="IV328_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938615.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IV328_002519"
     CDS             complement(597..1304)
                     /locus_tag="IV328_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938616.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IV328_002520"
     CDS             complement(1297..1734)
                     /locus_tag="IV328_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938617.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IV328_002521"
     CDS             complement(1749..2000)
                     /locus_tag="IV328_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938618.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IV328_002522"
     CDS             complement(2000..2206)
                     /locus_tag="IV328_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8938619.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IV328_002523"
     CDS             complement(2643..2903)
                     /locus_tag="IV328_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938620.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IV328_002524"
     CDS             2978..3181
                     /locus_tag="IV328_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8938621.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IV328_002525"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IV328_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8938622.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IV328_002526"
     CDS             complement(3810..4709)
                     /locus_tag="IV328_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8938623.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IV328_002527"
     CDS             complement(4722..5315)
                     /locus_tag="IV328_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP8938624.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IV328_002528"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IV328_002528"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8938625.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="IV328_002529"
     CDS             complement(7771..9231)
                     /locus_tag="IV328_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8938626.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="IV328_002530"
     CDS             complement(9292..11664)
                     /locus_tag="IV328_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938627.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2433 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtcc
//
LOCUS       DACDKJ010000083        11283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDKJ010000083 DACDKJ010000000
VERSION     DACDKJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.084"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..1874)
                     /locus_tag="IV328_002531"
     CDS             complement(138..1874)
                     /locus_tag="IV328_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8938628.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1871..2575)
                     /locus_tag="IV328_002532"
     CDS             complement(1871..2575)
                     /locus_tag="IV328_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8938629.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2706..3209
                     /locus_tag="IV328_002533"
     CDS             2706..3209
                     /locus_tag="IV328_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8938630.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3169..3507
                     /locus_tag="IV328_002534"
     CDS             3169..3507
                     /locus_tag="IV328_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8938631.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3528..4946
                     /gene="ffh"
                     /locus_tag="IV328_002535"
     CDS             3528..4946
                     /gene="ffh"
                     /locus_tag="IV328_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8938632.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            5058..5636
                     /locus_tag="IV328_002536"
     CDS             5058..5636
                     /locus_tag="IV328_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8938633.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5640..6161
                     /locus_tag="IV328_002537"
     CDS             5640..6161
                     /locus_tag="IV328_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8938634.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6240..6794)
                     /locus_tag="IV328_002538"
     CDS             complement(6240..6794)
                     /locus_tag="IV328_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8938635.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            7047..7322
                     /gene="rpsP"
                     /locus_tag="IV328_002539"
     CDS             7047..7322
                     /gene="rpsP"
                     /locus_tag="IV328_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8938636.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7334..7585
                     /locus_tag="IV328_002540"
     CDS             7334..7585
                     /locus_tag="IV328_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8938637.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7710..8501
                     /locus_tag="IV328_002541"
     CDS             7710..8501
                     /locus_tag="IV328_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938638.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8671..9192
                     /gene="rimM"
                     /locus_tag="IV328_002542"
     CDS             8671..9192
                     /gene="rimM"
                     /locus_tag="IV328_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8938639.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9182..9943
                     /gene="trmD"
                     /locus_tag="IV328_002543"
     CDS             9182..9943
                     /gene="trmD"
                     /locus_tag="IV328_002543"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8938640.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            10079..10426
                     /gene="rplS"
                     /locus_tag="IV328_002544"
     CDS             10079..10426
                     /gene="rplS"
                     /locus_tag="IV328_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8938641.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(10558..>11283)
                     /locus_tag="IV328_002545"
     CDS             complement(10558..>11283)
                     /locus_tag="IV328_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8938642.1"
                     /translation="SVIKSKLNLLFSYAMKKGYVEKNPINEVVIDYKRESKTIKIKDK
                     FLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITNNNASVVIN
                     GTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKGT
                     PFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENSDI
                     TEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         3692 a         2065 c         2295 g         3231 t
ORIGIN      
        1 ttttttgagt tacgtaaatg gaaagttttg tctcgcttaa tttgacaaat aatagagtaa
       61 aaaagaacaa ccactcgtgt atctttcgaa gcaggatgag tcatccaact ttaaaacgca
      121 cgagcagttg ctctttttta cattttcgga atggtaacgg tgaaagtcga acctgttcca
      181 agatgactgt ctacagtaat cgtaccatta aggagttgca cgtagtcttt tacaatcgct
      241 aaccctaatc cggtcccgcc tgaatgtcgg cttcgcgcct tgtctacgcg gtaaaaacgc
      301 tcaaagatcc gttcctgatc ctcaatgtcg atacctatac caaagtcttg gacagaaaac
      361 gatagatcat ctgttgcctg atagcggata ataatctcgc tgtcgctttt tgaatattgg
      421 acagcatttt caattagatt tttgataatt ggataaagga tatctgtttt agtagtaaat
      481 ggttcgttct ttccaatcaa tcgaatcgtc aaccgtttct tttcgatgat tggctgataa
      541 ttttgaacga tttgctggaa ataaggttct gtataaattg tctggatctc gtaagaattc
      601 tcgccatctt ttgacagctg gatgatttcc cgaatcagtt tatctaaccg gatcgcatct
      661 ttttgcatga tctcaagaaa agaagtcaga gtttgcgggt catcttttgc accatccagt
      721 aaagtctcgg taaaaccaat cagtgaagtc acaggagttt ttaattcgtg agagacgttg
      781 ccaacgaaat ccttttgtag cttttctaaa cgtcgcactt tcgttaagtc atacgccacc
      841 cctagaatct gtccggtacc ttctgaatta gtaaagtagc gcaaactcaa gtctaatgtc
      901 tgccgacctt ctgtaagggt gatctcctga tggatcagcg gatgctcagg agtcacttga
      961 tgaatcagct gaatgagttt tggctcttga atgacttctg tgtaccgctg ttcaggttga
     1021 tagcttacca cgtttaaatg tagctgcatc tttggattaa gcagtagtaa acgagaatca
     1081 gcatcatcaa tcaggaatac gccaatcatc agttcgttca gtaacgtata taattgctct
     1141 tctgtggtag tacacgcccg atacatctta ctcatttgtt cgctcaacgc attaatcgtc
     1201 tgatacaatt cttcccattg atcgggtgtt tgcatgatta cttccgcctg attaggattt
     1261 tgcaccattt tttttagtac gggcagcacg gttttcaatg gctggttttt ttgatagatc
     1321 agataataga tcattcctgc aagaacaagg aagaaagaca gaaagaagaa aaagacccaa
     1381 cggcgaaaac tctcagttct cggtaaaaaa ccgcttgtcg gttctgcgat acgtaaaatg
     1441 gcttctaact ggccactttt tttgactggt aatgcgacat ataaaagatc ctcattcaaa
     1501 gtagtacttt tgcgcaaagc gctacctagg ttcccaccat tcaatacggc tttgatttcg
     1561 ggacgggagt ttcgctgttc attcaatgtt tcattgtttg tgtcaaataa gatctttcca
     1621 gtagcatcca ttaaagtaat ccgttcgttc gactgatgga cgaattcttc caaagcagtt
     1681 ttgttttgcg ctgcttggag gttttcgaca tctaattgcc ggatcagcag ttgtgctttt
     1741 tttgtcaggt agctttcttg ctgttccacg atctgctgtt gaaaataatg ggaaatcaat
     1801 tgccagccac caaaaaacaa actaactaaa agaataagaa agaagagacg ttcataaatt
     1861 tttttccatt tcatcgtttg ggttcctgaa acttatagcc aaatcctcta atagtcacca
     1921 agtactttgg gtgttttgga tctcgctcga ttttttctcg caaatgacta acgtgtacat
     1981 ctacgattcg gctttgtcct gcgaagtcaa aattccaaat gcggtctagc aatgtgtctc
     2041 ggtcgatcac acggtctttt cgtttcatga aataaacaag taattcaaat tctttcggtg
     2101 ttagttcaat cagatggtcg tctacggtca cttgataatt ggtcaaatca gcagtgattt
     2161 gtccaatcga tagatattca ggctcagctt catcttttga ttgcttacga ggttcaattc
     2221 gtctgaatat tgctttcata cgggctaata cttcgcgagg actaaatggt ttggtcaaat
     2281 agtcgtcagc acctatttct aggccaatga tccgatccac ttggtcatct ttagccgtca
     2341 gcatcaaaat cggtgtttca tttttttctt gacgcagccg ctgagtgatc tccatgccat
     2401 ccatcgttgg aagcatcaca tctaaaatga tgaaatcaaa tgattgttct aatgctaatt
     2461 ctaatccttc gcctccatct gtggcactgg ttaccttgta gccttctttt tccaaattga
     2521 acgtcaagag cgttacaata gaaggttcat catcaacaac tagtattttt ttcattcaaa
     2581 tctcctttaa ataggtttca ttctgtttat attttagcat agaatcagta caaagggcta
     2641 ttttacaaaa gaaataaatg gtataatagg aaggcacttt gctgaataga aaggaagagc
     2701 agtcaatgat tggaatcagt gcatgtttag gcggtctggc ctgtcgctat gatggacaga
     2761 aaaaagaagt accagaattg atggaactag ttgaaactgg agaagctgtc atggtctgtc
     2821 cagaagtgat cggcggatta ccaattccta gagatcccgc tgaaatcatt ggtggaaatg
     2881 gattcgatgt gtggaaagat caagcaaaag tatggacgac aagcggcgaa gatgtaacgg
     2941 aagcgtacaa aaatggcgcg atcaaagcct atcaaaaatt gcaggaaaaa cacattgatc
     3001 tagtcatcat gaaggaaaaa agcccttcat gcggaaccca ttcgatttat gacggaagtt
     3061 tttccggaat aaaaaaaaat ggtgccggag tggctactgc ttattttatt cagcaaaaaa
     3121 tgactgtgct ctctgatgaa gaatggctga aaactagagg tgagcagaat ggaaatcgaa
     3181 aagaccaatc gaatgaacgc gctctttgaa ttttattcga ccttattgac agaaaaacaa
     3241 atgaactaca tggaattata ttatgcagat gatttttcat taggtgagat cgcggaagaa
     3301 tacgaagtaa gccgacaagc agtatatgac aatatcaagc ggacaagcaa gatcttggag
     3361 gattatgaaa aaaaactcca tcttttttct aattatattg ttcgagaaca ggtattggaa
     3421 aatatgactg catatattac agataagtac ccggaagata aaaaactact tgaatatgta
     3481 cagcaaattc aagcaatcga agaataaata aaaggaatga agaaaatatg gcatttgaaa
     3541 gcttaactga acgtctccag caggcgatgg gaaaaataag aaaaaaagga aaagtatcag
     3601 aagctgacgt aaaagaaatg atgcgggaaa tccgccttgc cttactagaa gccgatgtta
     3661 acttgcaagt agtcaaagat tttactaagc gcgtacgcga acgtgcggta ggggcagaag
     3721 tgctggaaag tttatcacct gcacaacaaa tcgtaaaaat cgttgatgaa gaattgacga
     3781 tcacacttgg ttctgaaaca gcagagttaa ataaatcacc aaaaatacca actgttatca
     3841 tgatggctgg tcttcaaggg gcaggtaaaa cgacctttac tggtaaatta gccaattatt
     3901 taaagaaaaa tgaaaatgct cgtccgctcc taatcgcagg ggacgtatat cgaccagctg
     3961 cgatcgacca attgaaagta ttaggtcaac agctagacgt tcctgtattt gatatgggaa
     4021 ctgaagtcag cccggtagag atcgtccgtc aagggatgga gttagcaaaa gaaaagaaaa
     4081 acgactatgt attgatcgat acggcaggac gtctgcatat cgatgaaaca ttgatggatg
     4141 agttgaaaca aatcaaagaa ttgacgcaac caaatgagat tttgcttgtt gtcgatgcca
     4201 tgactggtca agatgccgtc aacgtagctg atagcttcaa ccagcaatta ggtattacag
     4261 gggtcgtcat cactaaactt gatggggata ctcgaggcgg ggcagcacta tcgatccgct
     4321 cagtgacagg cgcaccaatc aaattcatcg gttctggtga aaaattgact gacttagaag
     4381 tattccaccc agatcgtatg gctagccgta tccttggtat gggggatatg ctgacattga
     4441 ttgaaaaagc gcagcaagac tacgacgaga aaaaagcgga agaacttgca cagaaaatgc
     4501 gggaaaattc attcgatttc aatgacttta ttgaacagct tgatcaagtg atgggtatgg
     4561 gcccactgga agatctaatc aaaatgatcc caggaatgaa tcaagtccct ggaatcgaaa
     4621 acgtcaaggt agatccaaaa gatgttgaac gcaaaaaagc gatggtttat tctatgacgc
     4681 cagcagaaag agcaaatcct gatctgttga acccaagccg tcgtcgccga attgctgccg
     4741 gttctggaaa cagtgttgtt gaagtgaatc gaatgatcaa acaattcaag gaatcaagaa
     4801 aaatgatgca gcaaatgtcc aaaggggata tgaacatccc aggtatggac caaatgtttg
     4861 gttcaggcgt caaaggaaaa ctaggcaaaa tggcgatgaa tcgaatgatc aagaaaaata
     4921 agaagaaaaa gaaaaaacgg aaataagatg aaaaaaacag cttcggtaca gactgaaaat
     4981 gtctactgcc ggagcttttc tatcagcaat ttttattctt tactaataca attactctaa
     5041 acgaagaggt ggcacagatg agatgtccgt ggagtgaaca aacagaaagt atgcggcaat
     5101 atcatgatac tgtctggggt gttcctgaat atgatgacca aaagctgttc cgaaaattga
     5161 tgcttgatat taatcaagcc ggattgagct ggcagacgat tttaaataaa agtgaagcat
     5221 tcgatgaagc atatgatcga ttcgatatca aaaaagtcgc aaattatgga gaaacaaaaa
     5281 tcagtgaact aatggaaaac aaaggaatta ttcgtaaccg aagaaaaatc gaagcagccg
     5341 tccaaaacgc caaagcagtc ttgaaaattc aagaagaaat cggcagtttt tcagagtatc
     5401 tttggtcgtt ttctaatgga aaaatcgttg attcttctta tcattcacaa gaagaagtcc
     5461 caacaacaag tcctctttca gatgaattag caaaagatct aaagaaaaga ggatttaaat
     5521 ttatcggaag cacaacaatc tatgcttttt tagaagctgt cgggatcatc aatgatcacc
     5581 ttgacagttg tttccgcaaa gaagaggtcg tacgtatcgg cagaaagggc gaataaagca
     5641 tgcgaaaaat catattttat ggtgctataa gtttagatgg ctatttggca acgaaaacgg
     5701 atgatcttca atggcttttt gacactccaa caggtaagca aactacttat gaagcttttt
     5761 atgaaacaat cgatacgacc atcatgggca gaaaaactta tcaggaagca aaaaaatatt
     5821 tggagaccga agcaatttat ccagagaaaa agaatttcgt tttttccacc cacgcacaat
     5881 tacaattgcc agatgctgag gtcgtaaacg aagatcctgt tgcttttcta aaaaatctac
     5941 aaatgacaga aggaaaagat atctgggttg tgggtggcgg tcgattgatc aaaccgctat
     6001 tggaacatca aatgattgat gaatggtata tccagattac ccctgtatta ttaggggacg
     6061 ggattccttt attccaaaaa ggaacatatg aagcgcgttt tgaaatgcta gacacgacac
     6121 gattcggtga atttatcgaa ttgcattatg tacgaaagta gaagtaaata aaataaaaaa
     6181 ggcaactata tgcagaaatg tcctgtacat agttgccttt tttattaccg ttatgctttt
     6241 tactctgtta atttgttaga aaaatctaga tacgcatcta aaatcgtttg gaagaaactt
     6301 aatgttcctt caacgatatt accattttca tcaattagat tcgcaacgtt tccgatatat
     6361 gcttccggtt gctgcaaggt tggcacattc aaaaagacaa gtgattggcg tagatggtgg
     6421 tttgcgccaa agccgccgat cccacctggt gatgcagtaa cgactaaacc tggtttatga
     6481 tcccaaacac tgtgtccata aggtcttgag cctacatcta aagcattttt caatactgct
     6541 ggcacagaac ggttgtattc aggagtaacg aaaacgacac cgtctagtcc cctcacttcc
     6601 tcacgaaaac gagtccattc agcaggagcc tgttctggag tttcgagatc ctcattgtaa
     6661 aatggcagat catcgatttt tacaaataca ctttcgtacc cttcaggaag caatttcgca
     6721 aaagcttctg ctactttttt gttgtaagaa tcttttctta aactaccaat aaaaaatcca
     6781 attttttttg tcatacgatt tttcctcctt caaactatct ataataatct tacaaaagat
     6841 aagtaactgg agcaaataaa aacacttact gacaaaagct acatagaaga tagattactt
     6901 atttttaaca aaaaagaagt ctaaaatctt ctattttcta aaaaaaatcg aatttaatca
     6961 agctgtaaag aaaaaaagct ttacagggta aaaaaaaact gttacactac atcttgtgaa
     7021 taaaacaatg gaggtggaaa taaataatgg cagttaaaat ccgtttaaaa cgtatgggtt
     7081 ctaaaaagag tccgttttac cgtatcgtcg tagctgattc acgttctcct cgtgatggac
     7141 gtttcatcga aactgtaggt acttacaatc ctttgaaaga ccctgcagaa gtagttttaa
     7201 aagaagactt agttcttgac tggttatcaa aaggtgcaca accttctgac actgtacgta
     7261 acatcctttc aaaagaaggc gttatgcaaa aacaccacga agctaaattc tcaaagaaat
     7321 aaggtgaccg gatatgaaag atttaagcga cttagtctta acaatcgttc gtccgctagt
     7381 cacttatcct gatcaagtca agttagacgt cgtagagtcg aaagactttt acgagtacaa
     7441 tctaacagtg gctcctgaag atattggccg tatcattgga aaacaagggc gtgttgctaa
     7501 agcaatccgc acaatcgttt acggtgttcg gatcaatgca ccgaaaaaag tgcgtttgaa
     7561 tatcatcgat gataaggaaa agtaaataaa cgtgctgtac tcgaattttc gagtgcagct
     7621 tttttgtttt ttttattaat tgaagaaatg attatttggc aaaacaagca agaaagcgta
     7681 tccttatagg aggagggagt gaacagccaa tgagaaagaa aatgttattt attccatttg
     7741 ctctattgtt aagcgcttgt agttcggtta ctcaagaatc agacaataca catctatcaa
     7801 gcagtatatc tgaaactgtt accagtccag aaaaaagtag taccaaaacg gcttccacca
     7861 ctgaacaaac aatggctacc tcaaataatg agaaaaagac tgctttagac cagttgaaag
     7921 aacaacagcc aaatgttccc atgcctctag atgtaccagt ctcttcaggc tatctcaata
     7981 tcgcagcgac acatacaaag caaggttatt ctattttata ttacagaaca gatcgtcccc
     8041 tcgggttgaa tgctgacgaa ttgaatcaag aaacgcctat tgcaacctat ctatatcaat
     8101 atgggtttgc ttctagtcaa gaaacaatcc aagttttgca gccatttgag atcgacacaa
     8161 atggtcagca agtggactta ggttctcgaa tcacagggta tcagcaaggt gcagcaggtt
     8221 caagttttct tgaatggcaa gaaggaaact ggcgtattcg aatccgtggg aataatatag
     8281 aaggccaaga tcccttacta ttagctaaag aaatcgtagc ttatttggaa gaaaacagtc
     8341 taccagcacc tgaacaattt ggtaaaatta ctgttgatat gggtgatacg acgaatcgag
     8401 ccgtagaagt aagctggcaa gaaccgaaaa atgcctatac gatcactcat caagatccta
     8461 tgtctgcgtt gaagatggca gtttctatga agaggttgtg aaatcagcgc gctgatctga
     8521 gcccatcatt taactccaag tcacaggaag taatcaaaaa tagcacgccg catttttgca
     8581 taattttggc ttaatacctt aaaagaaaac tagaattgcc tcttcacaca tgctaaaata
     8641 aaacagacaa aaaagaaatg gggatataag ttgactgaat acttgaatgt aggaaaaatc
     8701 gtaaacacac aaggtatcaa aggcgaagtc cgtgttatct caacaacgga tttcccagaa
     8761 gaacgttaca aaaaaggaac ggtgctaaca ctattccaag acggaaaagc acctgtggaa
     8821 ttaacagtca aaagtcatcg caaacataaa aattttgact tattgagttt tgaaggacat
     8881 ccttctatca atgatgtgga aaaatatcgg gatggcatat taaaagtgtc aaaagacaac
     8941 tctgtagatc tagctgaaaa tgaattctat taccatgaga tcatcggtct gcatgtatta
     9001 gaagatggaa ctgagttagg taaagtaaaa gaaatcttgt caccaggtgc caatgatgtt
     9061 tgggtcgttc aacgtccgaa aaaagctgat atattgcttc catatatcgc ttctgtcgtc
     9121 aaagaagtcg atttagaagc tggagtggtc cgtgtagaaa ttccagaagg actgattgac
     9181 gatgaagatt gatgtattga ctttatttcc aagaatgttt gaaggtccta tgggggaatc
     9241 catcatcggt aaggctgtag ataaaggatt attagatatc aatatctcta acttccgcga
     9301 ttattcagat aacaaacatc agacagtaga tgattatcca tacggcggtg gagcaggtat
     9361 gctgctcaaa gtccagccca tctatgataa catccaagcc attgaaaaag ctgcaccgga
     9421 tgtcaaaaaa agagtaattc ttcttgaccc agctggtaaa cgattcgatc agaaaatggc
     9481 agaagaattt tcacaagaag aacacttagt attcatttgt ggtcattatg agggatatga
     9541 tgaacgtatc cgcacattag tgacagatga agtttcctta ggagattatg tccttactgg
     9601 gggagaatta ggtgcgatgg tcatgatcga tgcaacagtt cggcttttgc cagatgtgtt
     9661 agggaatcag acttcagctc aaacggattc ttattcaacc ggtttattgg aacatcccca
     9721 atatactcgc ccggcagaat ataaagggat gaaagtacca gaagtattga caaatggaaa
     9781 tcataaattg attgaagaat ggcaattaaa agaatcacta agaaggacct atcaacgtcg
     9841 gcctgatctt ttggaaacac ttgaatggac acctcaaatg acgaaatttt tagaagaaat
     9901 caagaaagaa gaacaagaaa aaacagcaga tgaagccaag tgactttttc taaaatcttc
     9961 tttacagtag gttgataata tgttagtatt ttatggtgag tgcaaaagca cttaactatt
    10021 acgatattcc gctgtgagaa aagctcataa gaatatttgg aataaggaga agaaaaccat
    10081 gaatccatta atcgaagaat taacaaaaga acaattacgt tctgacatcc cagcgttccg
    10141 ccctggtgac actgtgcgtg ttcacgcaaa agttgttgaa ggtactcgtg aacgtatcca
    10201 gttatttgaa ggtgttgtta tcaaacgccg cggtgctgga atcagcgaaa cttatacagt
    10261 acgtaaagtt tctaacggag tgggcgttga acgtacattc ccattgcata caccacgtgt
    10321 tgcaaaaatc gaagtagttc gctacggtaa agtacgtcgt gcaaaattgt actacctacg
    10381 tgcattacac ggaaaagctg ctcgtatcaa agaaatccgt cgttaatcat cgttatcaga
    10441 ctgagaaaga acccttggta ttcaagggtt ctttttttgt tctctgtttt tttatattaa
    10501 cgatttctat ttgggacatt tttgattaat aaaaaggggc aaaaaagggg caacaagtta
    10561 cagtttttct atatcttctt tcaatttttc ttttactccc tttgtcacat gcaaataaat
    10621 cttttcagtg atatcactat tttcgtgccc aacgcgatcc tgaatagcat acagaggagt
    10681 tcctagttcc gctaattttg aaatatgggt atgtctaaaa atatgtgaac taagtttttt
    10741 atcaatcttc atgtcagctt tatgatttct caaataggtg ttaattgctg ttagttggaa
    10801 aggagttcct ttcgttgttt gaaatagaaa ttgaccattc ggatttaatt ctagaagctc
    10861 attgtaaata gctatcgctt tttttggtaa atcaatttct cgcattccag cagcagtttt
    10921 tgtagaatca gattttttca tatcagctat tgaacgttct ctatacatca tcgtcccatt
    10981 tataactact gatgcattat tattggtaat gtgtacatcg tctttactta atgcgattgc
    11041 ttctccaggc ctcattccag tcaaatatag ccactggaaa aggagagaat atcttttatt
    11101 gtgtgaagtt gtgaaattta ctagtctttt atattcatca tcttctaaaa acttatcttt
    11161 aatttttatc gtttttgact ctcgtttata atcaataact acttcgttga taggattttt
    11221 ttctacatag ccttttttca tagcgtatga aaaaagaaga ttcaatttgg atttaattac
    11281 act
//
LOCUS       DACDKJ010000084        10928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDKJ010000084 DACDKJ010000000
VERSION     DACDKJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.085"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..287)
                     /locus_tag="IV328_002546"
     CDS             complement(<1..287)
                     /locus_tag="IV328_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8938643.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="IV328_002547"
     CDS             429..1547
                     /locus_tag="IV328_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8938644.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="IV328_002548"
     CDS             1675..3051
                     /locus_tag="IV328_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8938645.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="IV328_002549"
     CDS             3452..5032
                     /locus_tag="IV328_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8938646.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="IV328_002550"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="IV328_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="IV328_002551"
     CDS             complement(5658..5978)
                     /locus_tag="IV328_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8938647.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="IV328_002552"
     CDS             complement(6126..8360)
                     /locus_tag="IV328_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8938648.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="IV328_002553"
     CDS             8839..9105
                     /locus_tag="IV328_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8938649.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="IV328_002554"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="IV328_002554"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8938650.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2215 g         3322 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 agacaaaa
//
LOCUS       DACDKJ010000085        10654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDKJ010000085 DACDKJ010000000
VERSION     DACDKJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.086"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IV328_002555"
     tRNA            8..81
                     /locus_tag="IV328_002555"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IV328_002556"
     tRNA            101..173
                     /locus_tag="IV328_002556"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IV328_002557"
     tRNA            201..274
                     /locus_tag="IV328_002557"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IV328_002558"
     tRNA            288..361
                     /locus_tag="IV328_002558"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IV328_002559"
     tRNA            385..474
                     /locus_tag="IV328_002559"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IV328_002560"
     tRNA            487..560
                     /locus_tag="IV328_002560"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IV328_002561"
     tRNA            579..654
                     /locus_tag="IV328_002561"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IV328_002562"
     tRNA            656..726
                     /locus_tag="IV328_002562"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IV328_002563"
     tRNA            740..813
                     /locus_tag="IV328_002563"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IV328_002564"
     tRNA            838..925
                     /locus_tag="IV328_002564"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IV328_002565"
     tRNA            947..1018
                     /locus_tag="IV328_002565"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IV328_002566"
     tRNA            1041..1130
                     /locus_tag="IV328_002566"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IV328_002567"
     tRNA            1143..1216
                     /locus_tag="IV328_002567"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IV328_002568"
     tRNA            1235..1307
                     /locus_tag="IV328_002568"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IV328_002569"
     tRNA            1311..1383
                     /locus_tag="IV328_002569"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IV328_002570"
     tRNA            1388..1458
                     /locus_tag="IV328_002570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IV328_002571"
     tRNA            1471..1544
                     /locus_tag="IV328_002571"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IV328_002572"
     tRNA            1556..1627
                     /locus_tag="IV328_002572"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IV328_002573"
     CDS             1893..3086
                     /locus_tag="IV328_002573"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8938651.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IV328_002574"
     CDS             3216..4688
                     /locus_tag="IV328_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8938652.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IV328_002575"
     CDS             4771..5559
                     /locus_tag="IV328_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8938653.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IV328_002576"
     CDS             complement(5626..6294)
                     /locus_tag="IV328_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8938654.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IV328_002577"
     CDS             6409..8121
                     /locus_tag="IV328_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938655.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IV328_002578"
     CDS             8124..9896
                     /locus_tag="IV328_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938656.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(9978..>10654)
                     /locus_tag="IV328_002579"
     CDS             complement(9978..>10654)
                     /locus_tag="IV328_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8938657.1"
                     /translation="GLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKS
                     SHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGS
                     YRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELN
                     LDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEK
                     LGKIEI"
BASE COUNT         3107 a         1873 c         2373 g         3301 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag ttgattttgc
    10021 atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc atgtcctaca
    10081 cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat gtgggaatgc
    10141 cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac tgctcttaaa
    10201 gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa aatgtattta
    10261 tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat ttctttagca
    10321 ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt tttaattttt
    10381 aactcgtaat cataagtacc agaaaccgta attttattac catcataatc agattcagtc
    10441 aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt aattaaagaa
    10501 taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac ttcttctttt
    10561 gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt tcttgctaaa
    10621 gaaaccccat ttacgggatt atattttaat ccat
//
LOCUS       DACDKJ010000086        10532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDKJ010000086 DACDKJ010000000
VERSION     DACDKJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.087"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IV328_002580"
     CDS             complement(25..144)
                     /locus_tag="IV328_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8938658.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IV328_002581"
     CDS             complement(159..1343)
                     /locus_tag="IV328_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8938659.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IV328_002582"
     CDS             complement(1599..1853)
                     /locus_tag="IV328_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938660.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IV328_002583"
     CDS             complement(1878..3020)
                     /locus_tag="IV328_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8938661.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IV328_002584"
     CDS             complement(3080..4432)
                     /locus_tag="IV328_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8938662.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IV328_002585"
     CDS             complement(4503..4847)
                     /locus_tag="IV328_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8938663.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IV328_002586"
     CDS             complement(4857..5231)
                     /locus_tag="IV328_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8938664.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IV328_002587"
     CDS             complement(5244..5558)
                     /locus_tag="IV328_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8938665.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IV328_002588"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IV328_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IV328_002589"
     CDS             complement(8976..9638)
                     /locus_tag="IV328_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938666.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="IV328_002590"
     CDS             10255..10485
                     /locus_tag="IV328_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938667.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2895 a         2344 c         1786 g         3507 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ct
//
LOCUS       DACDKJ010000087        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDKJ010000087 DACDKJ010000000
VERSION     DACDKJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.088"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IV328_002591"
     CDS             complement(247..759)
                     /locus_tag="IV328_002591"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8938668.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IV328_002592"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IV328_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8938669.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IV328_002593"
     CDS             complement(3212..3646)
                     /locus_tag="IV328_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8938670.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IV328_002594"
     CDS             complement(3656..4444)
                     /locus_tag="IV328_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8938671.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IV328_002595"
     CDS             complement(4441..5382)
                     /locus_tag="IV328_002595"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8938672.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IV328_002596"
     CDS             complement(5559..6146)
                     /locus_tag="IV328_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8938673.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IV328_002597"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IV328_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8938674.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IV328_002598"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IV328_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8938675.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IV328_002599"
     CDS             complement(9063..9998)
                     /locus_tag="IV328_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8938676.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACDKJ010000088         9876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDKJ010000088 DACDKJ010000000
VERSION     DACDKJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.089"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            201..680
                     /locus_tag="IV328_002600"
     CDS             201..680
                     /locus_tag="IV328_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938677.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="IV328_002601"
     CDS             713..2941
                     /locus_tag="IV328_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8938678.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="IV328_002602"
     CDS             3070..4713
                     /locus_tag="IV328_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8938679.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="IV328_002603"
     CDS             complement(4786..5331)
                     /locus_tag="IV328_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8938680.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="IV328_002604"
     CDS             complement(5354..5839)
                     /locus_tag="IV328_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8938681.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="IV328_002605"
     CDS             complement(5829..6641)
                     /locus_tag="IV328_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8938682.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="IV328_002606"
     CDS             complement(6780..8312)
                     /locus_tag="IV328_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8938683.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="IV328_002607"
     CDS             complement(8421..9452)
                     /locus_tag="IV328_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8938684.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3281 a         1833 c         1904 g         2858 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcagcgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa tagttggtac
     9841 ttcattactt tacatgaaga tttattctat ggtagg
//
LOCUS       DACDKJ010000089         9572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDKJ010000089 DACDKJ010000000
VERSION     DACDKJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.090"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(92..760)
                     /locus_tag="IV328_002608"
     CDS             complement(92..760)
                     /locus_tag="IV328_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8938685.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="IV328_002609"
     CDS             894..1181
                     /locus_tag="IV328_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938686.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1597..3135
                     /locus_tag="IV328_002610"
     CDS             1597..3135
                     /locus_tag="IV328_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8938687.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3132..4097
                     /locus_tag="IV328_002611"
     CDS             3132..4097
                     /locus_tag="IV328_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938688.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4084..5406
                     /locus_tag="IV328_002612"
     CDS             4084..5406
                     /locus_tag="IV328_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8938689.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5469..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5647..6390
                     /locus_tag="IV328_002613"
     CDS             5647..6390
                     /locus_tag="IV328_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8938690.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6405..7397
                     /locus_tag="IV328_002614"
     CDS             6405..7397
                     /locus_tag="IV328_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8938691.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7409..8146
                     /locus_tag="IV328_002615"
     CDS             7409..8146
                     /locus_tag="IV328_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938692.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8136..8858
                     /locus_tag="IV328_002616"
     CDS             8136..8858
                     /locus_tag="IV328_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8938693.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8942..9565)
                     /locus_tag="IV328_002617"
     CDS             complement(8942..9565)
                     /locus_tag="IV328_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8938694.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3138 a         1642 c         2018 g         2774 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1441 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1501 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1561 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1621 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1681 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1741 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1801 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1861 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1921 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1981 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2041 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2101 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2161 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2221 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2281 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2341 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2401 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2461 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2521 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2581 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2641 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2701 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2761 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2821 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2881 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2941 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3001 aaattaagcg gaaaaataac ggaggatcaa atgattgata tgaattacca agtcaatgtc
     3061 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3121 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3181 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3241 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3301 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3361 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3421 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3481 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3541 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3601 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3661 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3721 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3781 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3841 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3901 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3961 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4021 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4081 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4141 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4201 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4261 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4321 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4381 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4441 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4501 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4561 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4621 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4681 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4741 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4801 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4861 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4921 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4981 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5041 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5101 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5161 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5221 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5281 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5341 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5401 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5461 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5521 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5581 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5641 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5701 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5761 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5821 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5881 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5941 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6001 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6061 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6121 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6181 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6241 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6301 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6361 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6421 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6481 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6541 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6601 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6661 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6721 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6781 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6841 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6901 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6961 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7021 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7081 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7141 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7201 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7261 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7321 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7381 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7441 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7501 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7561 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7621 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7681 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7741 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7801 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7861 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7921 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7981 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8041 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8101 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8161 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8221 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8281 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8341 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8401 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8461 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8521 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8581 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8641 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8701 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8761 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8821 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8881 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8941 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9001 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9061 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9121 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9181 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9241 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9301 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9361 aaatcaggtt aatatcagaa attgaatttg attctgcaat ggcattgatc aaggagttat
     9421 caccaacgta tttttgagaa atcgttgata gagtatcacc tgattgaacg gtataagctt
     9481 cgtccgcatg agcagtcgtt cccatgaaga atgcagcgcc ggcagccaaa gttgttccaa
     9541 aaagtaaagt tttaagtgat gtcatgaaaa at
//
LOCUS       DACDKJ010000090         8970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDKJ010000090 DACDKJ010000000
VERSION     DACDKJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.091"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(553..2166)
                     /locus_tag="IV328_002618"
     CDS             complement(553..2166)
                     /locus_tag="IV328_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8938695.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="IV328_002619"
     CDS             complement(2307..3215)
                     /locus_tag="IV328_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8938696.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3398..4522
                     /locus_tag="IV328_002620"
     CDS             3398..4522
                     /locus_tag="IV328_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8938697.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4749..5666
                     /locus_tag="IV328_002621"
     CDS             4749..5666
                     /locus_tag="IV328_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8938698.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5659..6492
                     /locus_tag="IV328_002622"
     CDS             5659..6492
                     /locus_tag="IV328_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8938699.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6575..7519
                     /locus_tag="IV328_002623"
                     /pseudo
     CDS             6575..7519
                     /locus_tag="IV328_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
     gene            7645..8937
                     /locus_tag="IV328_002624"
     CDS             7645..8937
                     /locus_tag="IV328_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8938700.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT         2949 a         1539 c         1672 g         2810 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
     3301 aatatttttt tatataaata tttgtgttac taattccgtt aatatacata agtgaaagcg
     3361 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     3421 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     3481 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     3541 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     3601 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     3661 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     3721 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     3781 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     3841 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     3901 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     3961 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     4021 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     4081 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     4141 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     4201 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     4261 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     4321 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgccaa
     4381 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     4441 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     4501 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     4561 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     4621 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     4681 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     4741 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     4801 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     4861 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     4921 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     4981 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     5041 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     5101 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     5161 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     5221 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     5281 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     5341 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     5401 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     5461 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     5521 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     5581 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     5641 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     5701 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     5761 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     5821 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     5881 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     5941 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     6001 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     6061 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     6121 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     6181 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     6241 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     6301 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     6361 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     6421 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     6481 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     6541 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     6601 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     6661 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     6721 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     6781 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     6841 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     6901 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     6961 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     7021 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     7081 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     7141 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     7201 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     7261 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     7321 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     7381 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     7441 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     7501 agctgctatc aatatactag ctctatactt tttgatgttg gtggatgaca ccagcatctt
     7561 ttttattttc caaaacatat agaaaagaca ccttgaaatc ctttagaatt aatttggcga
     7621 accaacccaa aaggaggaat caagatgtct tacactcacc ttatcaaaga aacattagat
     7681 attctagact tatcagttac ttttaacgaa aattgtctca caaaagaaaa atacaaaggg
     7741 caaatctgtc atatttatcg tggaaacttg atttatacag cccaagaatg catccactgt
     7801 aaacaccaga ttgcttcaga tattgtgcgt tggggtacaa caaccgtccg gttgttaatg
     7861 aacgatgttt ctgaatgccg tacctacctc gaattgaaaa agcaacgctt caaatgcaaa
     7921 gcttgtcagc ggacctttgt cgcagatact tctgttgcga aaaaacactg tttcatcagt
     7981 gagaaagtcc gctggtctgt cgtgactcgt ctgaaaaaaa atacctcaat gacagagatc
     8041 gccgctcaga aaaatctatc ggtctcttct gtttaccgta ttatgaagcg attttatcgt
     8101 ccgttgaatc cttttcggac accgttacct aaggtacttt gttttgatga attcaaatca
     8161 gttcggggtg tttcaggtgc tatgagtttc atcatgatgg atggacaaac acagcgcttg
     8221 ttggacattg tcgagaaccg acaactgcct tttttaaaac gttattttag tcatttttca
     8281 cgagaaattc gtgaagcggt cgaatggatc gtgattgata tgtacgctcc ttatgtatcg
     8341 ctcgttaaaa agctgtttcc aaaagcacag ctgatcattg accggtttca catcgtccaa
     8401 catattggtc gtacctttcg caatcatcga atcaaagaaa cgaaccaact attaaaaagc
     8461 aaagaacaaa aacactatca attaggtaag caattaaaaa gatactggaa acttcttcaa
     8521 aaagatgaac gcaagcttga ttatactcga cgcctttggc gacctggctt caaagctcat
     8581 ttaacagaga ccgatattgt ggatcgctta ctgaaaggga gccctgcttt gcgtgtaggc
     8641 tatcagttat atcaagactt tctttacgct gtaaaggaac gagactacgt atcgtttgaa
     8701 gaactactta ccaataatat catgcttccc gaaggctatc agacgacctt gagaacgttc
     8761 cagaaatttc tgccacaaat caaaaatgct ttacaacaaa gttattcaaa tggcccactt
     8821 gaatgtctga acaaccatat caaagtgtta aaacgaaatg catatggctt tcgtagcttt
     8881 tataatttca aattgcgcat catgattcgt catggcaacg cattgatttt caactaaaaa
     8941 agatcaagag gaactgatcc acttgatctc
//
LOCUS       DACDKJ010000091         8919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDKJ010000091 DACDKJ010000000
VERSION     DACDKJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.092"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="IV328_002625"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="IV328_002625"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8938701.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IV328_002626"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IV328_002626"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8938702.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4432)
                     /locus_tag="IV328_002627"
     CDS             complement(3191..4432)
                     /locus_tag="IV328_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8938703.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="IV328_002628"
     CDS             complement(4450..5373)
                     /locus_tag="IV328_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8938704.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="IV328_002629"
     CDS             complement(5594..8086)
                     /locus_tag="IV328_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8938705.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="IV328_002630"
     CDS             complement(8093..8560)
                     /locus_tag="IV328_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8938706.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8696..8769)
                     /locus_tag="IV328_002631"
     tRNA            complement(8696..8769)
                     /locus_tag="IV328_002631"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8733..8735),aa:Arg,seq:acg)
     gene            complement(8827..8900)
                     /locus_tag="IV328_002632"
     tRNA            complement(8827..8900)
                     /locus_tag="IV328_002632"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8864..8866),aa:Pro,seq:tgg)
BASE COUNT         2478 a         1884 c         1485 g         3072 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc
     8701 ccaagaggag tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
     8761 tatgggcgca tattcaattt aaaaaaatta taaatcattt gaaaacaaaa agcaagataa
     8821 aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     8881 ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DACDKJ010000092         8851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDKJ010000092 DACDKJ010000000
VERSION     DACDKJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.093"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..1277
                     /locus_tag="IV328_002633"
                     /pseudo
     CDS             63..1277
                     /locus_tag="IV328_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1791)
                     /locus_tag="IV328_002634"
     CDS             complement(1456..1791)
                     /locus_tag="IV328_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8938707.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="IV328_002635"
     CDS             complement(1775..2047)
                     /locus_tag="IV328_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8938708.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="IV328_002636"
     CDS             complement(2498..3301)
                     /locus_tag="IV328_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8938709.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="IV328_002637"
     CDS             complement(3279..4403)
                     /locus_tag="IV328_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8938710.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="IV328_002638"
     CDS             complement(4419..5234)
                     /locus_tag="IV328_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8938711.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6105)
                     /locus_tag="IV328_002639"
     CDS             complement(5224..6105)
                     /locus_tag="IV328_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8938712.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="IV328_002640"
     CDS             complement(6181..7470)
                     /locus_tag="IV328_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8938713.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="IV328_002641"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="IV328_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2742 a         1883 c         1410 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DACDKJ010000093         8691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDKJ010000093 DACDKJ010000000
VERSION     DACDKJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.094"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            123..515
                     /locus_tag="IV328_002642"
     CDS             123..515
                     /locus_tag="IV328_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938714.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="IV328_002643"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="IV328_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            857..1054
                     /locus_tag="IV328_002644"
     CDS             857..1054
                     /locus_tag="IV328_002644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938715.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="IV328_002645"
     CDS             complement(1097..1672)
                     /locus_tag="IV328_002645"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8938716.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="IV328_002646"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="IV328_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            2810..4321
                     /gene="gadC"
                     /locus_tag="IV328_002647"
     CDS             2810..4321
                     /gene="gadC"
                     /locus_tag="IV328_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP8938717.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4334..5734
                     /locus_tag="IV328_002648"
     CDS             4334..5734
                     /locus_tag="IV328_002648"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP8938718.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5899..7339)
                     /locus_tag="IV328_002649"
                     /pseudo
     CDS             complement(5899..7339)
                     /locus_tag="IV328_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7732..8325
                     /locus_tag="IV328_002650"
                     /pseudo
     CDS             7732..8325
                     /locus_tag="IV328_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
     gene            complement(8309..>8691)
                     /locus_tag="IV328_002651"
     CDS             complement(8309..>8691)
                     /locus_tag="IV328_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8938719.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         2759 a         1447 c         1567 g         2918 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
     2461 tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc
     2521 tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat acgatataaa
     2581 acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg ttgtcagttt
     2641 tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat tttgcttgct
     2701 atactttgtc ttgtagaaaa gaggtggaca aaataatgca agctcaaata agcgattttc
     2761 cataaccttg gtaggaatga caaattaaat aggaaaggga agtaataata tgaataaaaa
     2821 aaagttatct ctattcggtt tttttgccct gacagcttcc atggttttga ctgtttatga
     2881 gtatccgacc tttgcaacat cacaattaca tttggttttc tttcttcttt tgggaggtat
     2941 tttatggttt ctaccagttg ctttatgtgc tgcagaaatg gcaacagttg aaggatggaa
     3001 agatggtggg attttcagtt gggttagtca aactttagga gaacgttttg gttttgcagc
     3061 tatttttttc caatggtttc aaattacagt aggttttatt acaatgatct acttcatact
     3121 aggagcgctg tcgtatgtaa ttaattttcc agagcttaat acagatccat ttatcaagtt
     3181 tattggtgta ttagtcattt tctgggtact gactttttca cagttgggcg ggaccaatcg
     3241 taccgctaaa attgctaaag caggatttgt cattggaatt gttattcctt cgatcattct
     3301 ctttggttta gcagcagcct actttatcgg cggtaatccg attcaaatgg ctattaatgc
     3361 gaaagctttt attcctgact tttctcagct ttcaacttta gtagtatttg tatcttttat
     3421 attggcttat atgggcgtag aagcttcagc atcacatatt aatgagctgg aaaatcccca
     3481 aagaaactac ccattggcaa tgtttttact agtgattttg gccatcgcct tagatgcaat
     3541 cggcggtttc tcagtagcag cggttattcc acaacatgac ttgtctctca gtgctggtgt
     3601 aatccaaaca tttgaaacgc ttattttaca ttttaacagt catttacatt ggttggtaaa
     3661 agtgattgct ctcatgattg cctgtggcgt tatgggtgaa gttagttctt gggtagttgg
     3721 tccatcacga gggatgtttg cagctgctca gcgaggtctg ttaccagttt tcttccggaa
     3781 aactaataaa caagacgttc cagttccgtt gattatgata caaggaatta tcgttactat
     3841 ttgggcagca gtcttaactt ttggtggagg aggcaacaat ttatcattct tagtagcaat
     3901 ctccttaact gttgtcattt atctcgtagg atatttacta tttttcctag gttactttgt
     3961 attagttttt aagaaaaaag acttaaaacg aacttacaat gtaccaggaa aaactgttgg
     4021 caaaacgatt atcggtggaa ttggttttat tttatcagtg ttcgcattgc ttatctcttt
     4081 tgtaccacca gcttcaattg cagctagtga gcaccgaaca taccaaatca tactacttat
     4141 tagctttgta gtgactgttc ttttaccttt tatcatttat gaagtacatg ataaaagtgc
     4201 tcacacaact attgaggaac ctactcacgt gaaagctgaa gacgttaatc ccgctgttta
     4261 tccagctgct agaggagagc accacgttac aaaaaatgat gaacatattt taaaacatta
     4321 ggaggaattc attatgttat acggaaaaga taatcaagaa gaaaaaaact atttggaacc
     4381 aatttttggc tctgcaagtg aggatgttga cttgcctaaa tataagttaa acaaagaatc
     4441 cattgaacca cgaattgctt atcaattagt acaagacgag atgttggatg aaggaaatgc
     4501 gcgattaaac ttagctactt tttgtcaaac gtatatggaa cctgaagcag tgaaattgat
     4561 gacccaaacg ttagaaaaaa atgcaattga taaatcagaa tacccacgaa caacggaaat
     4621 tgaaaaccgc tgtgtaaata tgattgctga tttatggcat gctccaaata atgaaaaatt
     4681 catgggaact tcaacgatcg gctcttcaga agcctgcatg ctgggtggta tggccatgaa
     4741 atttgcttgg cgtaaacgtg ctgaaaaatt aggtcttgat attcaagcaa aaaaacctaa
     4801 cctggtgatc tcttctggtt accaagtttg ttgggaaaaa ttctgtgtat attgggatgt
     4861 ggaactgaga gaagtcccaa tggatgaaaa acatatgtca attaatctag atactgtcat
     4921 ggattatgtg gatgagtaca caattggtat tgtaggtatt atgggtatta cttacactgg
     4981 tcgttatgat gatatcaagg gtctgaatga tttagttgaa gctcacaata aacaaactga
     5041 ctataaagta tacattcatg ttgacgctgc atcgggtggc ttttatgcac catttactga
     5101 acctgatcta gtttgggatt ttcaattgaa aaatgttatc tcaattaatt cttcaggtca
     5161 caaatatggt ttggtatatc caggtgtggg ttgggtctta tggcgtgacc aacaatactt
     5221 accagaagaa ttagtattta aagttagtta cttaggtgga gaaatgccaa ctatggctat
     5281 caacttctct catagtgcag cacaactaat tgggcaatac tacaactttg tacgctatgg
     5341 ctttgatggt tatcgtgata ttcaccaaag aactcatgat gttgctgttt atttagccaa
     5401 agagatcgaa aaaactggta tttttgaaat cattaatgat ggatcagaat tgccagttgt
     5461 gtgctataag ctgaaagaag atcccaatcg cgaatggaca ctatatgatt tatctgatcg
     5521 tctgttaatg aagggatggc aagtcccagc ctacccatta cctaaagact tggatcaatt
     5581 aattattcaa cgcttagttg ttcgagcaga ctttggaatg aacatggctg gtgattatgt
     5641 acaagatatg aaccaagcaa ttgaagagtt gaataaagct catattgttt atcataaaaa
     5701 acaggatgtg aaaacgtacg gctttactca ctaaaattct tcagagaaat ataaaagaag
     5761 ttaattaact aataaaagga gttgtgatta ccacacagct ccttaaatct atttgaataa
     5821 atatatgata ttcatttgtt acactctcaa tcttagctgt tgttctattc atcattactc
     5881 ttattgtaag tagttcagtc aaatttgctt gttcaattga tcgataacag tatgaattgt
     5941 ttgaaaagta atcggatata taattgtttt ctcactacct aagccttctt ggtataaaga
     6001 actatcgcaa agtataatcc gctgagtaca aaagaaagtg tcgtctacta tttctctaag
     6061 ttgtttacaa atgatattac taatagaaaa gtgttctccc aaaatgtctt ctaactgttg
     6121 tcgatagtat aaatacctaa agtctgacgg agctactatg aacacttcaa ttggagattt
     6181 ccgtttatgc tcatttaaaa gacttaaact aatggttagt ttacgaatag aattctcatt
     6241 ccaacgcaga aataaattgt tttgttttcg ccattgttca agaatttcat gaacttctaa
     6301 gtacaaagcg cgttgctctt catttaacaa gtaagtctta tcaggttgaa atatctgaat
     6361 atcagcccaa agtgagcgga taaacgggag aagtgcttta tcaaaaagta tctgattttc
     6421 ttcagatata ttcagagata agataagttt ttgactgagc tcaatcacca aagggttatc
     6481 tgctacatgg ttttgataaa caacgttcaa gtctttttgt aacaattcaa agttatcgca
     6541 tagataattt ctagagttga atagaaagta gatataaagg cattcatctt cgtcatgaca
     6601 taaagagaaa gaaagttgct ctaatccttt ctgtattagc tgaaacaagg gagtatgtcg
     6661 ggcaactctt ttttcttcct gactaaaata tattgggtta gtcttagatc tctgcaaacc
     6721 gatagcgata cctaataaaa taaattgttt ttcaactgta gggtaacttc ttgaaaaaaa
     6781 atgtttttct acataatcaa tcaattgttg accgctttga ataattgatt tatttagctg
     6841 gatcaactct gtccgatttg tctcatagat tatcgttagt aacaaacttc gataatcctt
     6901 ttcaggaatt tcaaaatggc catcagcatt taagtactgc atttgtttaa aaaaattaat
     6961 caactcattt cgtaagttgt aaactttgct taacgaaatg aaggtagtat cagctatatt
     7021 actccaattg tgttcccctg ataaataatg tcctagtaca ttgaaaaagt cagagcgctg
     7081 atagatgagt tgagtgagtg ccaacctcga aaacgaagag tcgaatgcga ctgtaaagct
     7141 acttctagat ttctccactg aaataatttg ctgatggagt atgtcggtaa tgcattccaa
     7201 gtcgttagtg acagacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     7261 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     7321 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataactc
     7381 taccaatcgg aatagtcctg taatatattg aatcaaaaat ataaattata aaataattag
     7441 atggggtatt tggtttaagt atcccttata tagtaataaa tttataggtg tgattctcga
     7501 attaataccc gaaaatgtga gagaattttt atggattttt taggatacaa attaattatt
     7561 tttgctttta tcctgattac cagtccagta ctgtcggtat cccattttgc agcaaaatca
     7621 tgttgataaa gtgacaagaa gactgatgag atatattatc caaattgaga agataattaa
     7681 tcggattctt gagtataccc tactttttaa gaaagtaggg tatacaagcg tatgaataaa
     7741 gacataactt tccctgtgat ggacgttcgg cttgttccaa ttaccaaaat acaggcgaac
     7801 gattataatc ccaatcgagt agcgtctccg gaaatgaact tattggagct ttctatcatt
     7861 gaagatggct ttactcaacc attagtttgt tattacaagg aaaaagaaga gaagtatatc
     7921 ttggttgatg tgcttcatag gtatcgtgtt gctaaggaaa gactcaactt aaaagttgta
     7981 ccggtaacag ttattgataa acccatataa gaacgtatgg cttcaacaat tcgacacaat
     8041 cgtgcaagag ggacccatga aataagagga atggaaattg tcatccaaag attagtaaaa
     8101 aatggatggt cagacaagag aatatccaaa gaattaggca tggacattga agaaattttt
     8161 cgctttaagc aagtgtctgg tggaatggca acagtttttt tgacaaattt tataaggtgc
     8221 agaacttctt tccgtatgct attccgattg tccttgacaa tgagcctcct cggatgtgac
     8281 gatcttcggc aggagctaac agttaaagtt agactgcctc gctagcgtta gcacatccga
     8341 gctcattatc aaggattcgc tgcgccaatc tctggttacg gtaaccaacc ggtgtctcgt
     8401 agccaagtgt accgtgcaac cgaaggtggt tccaccaatt tacatagtca aataactcca
     8461 aatccaattg ttgtaaggtt tcaaatgtgt attgatagac aaattctact ttcaacgact
     8521 tataagttga ttcagctacg gcattatcaa aaggacagcc tttatgactc aatgatcgat
     8581 tgatgtcaaa agttgttaat aattcatcaa tagcttggtt atcaaactct tttccacgat
     8641 cagtatgaaa aatctcaacc tctgtcagag gttgtttgat acggctaaat g
//
LOCUS       DACDKJ010000094         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDKJ010000094 DACDKJ010000000
VERSION     DACDKJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.095"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IV328_002652"
     CDS             complement(<1..173)
                     /locus_tag="IV328_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8938720.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IV328_002653"
     CDS             complement(593..1714)
                     /locus_tag="IV328_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8938721.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IV328_002654"
     CDS             2042..3451
                     /locus_tag="IV328_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8938722.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IV328_002655"
     CDS             3448..4176
                     /locus_tag="IV328_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8938723.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IV328_002656"
     CDS             complement(4304..5215)
                     /locus_tag="IV328_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8938724.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IV328_002657"
     CDS             complement(5232..6239)
                     /locus_tag="IV328_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8938725.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IV328_002658"
     CDS             complement(6236..8365)
                     /locus_tag="IV328_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8938726.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IV328_002659"
     CDS             complement(8370..8477)
                     /locus_tag="IV328_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938727.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDKJ010000095         7662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDKJ010000095 DACDKJ010000000
VERSION     DACDKJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.097"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..819
                     /locus_tag="IV328_002660"
     CDS             <1..819
                     /locus_tag="IV328_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8938728.1"
                     /translation="WIDIKYFAIQKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDT
                     WKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINR
                     VKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHS
                     PSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQ
                     NISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            809..1549
                     /locus_tag="IV328_002661"
     CDS             809..1549
                     /locus_tag="IV328_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8938729.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1546..4308
                     /locus_tag="IV328_002662"
     CDS             1546..4308
                     /locus_tag="IV328_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8938730.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            4321..5229
                     /locus_tag="IV328_002663"
     CDS             4321..5229
                     /locus_tag="IV328_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8938731.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            5229..5849
                     /locus_tag="IV328_002664"
     CDS             5229..5849
                     /locus_tag="IV328_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938732.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            5853..6302
                     /locus_tag="IV328_002665"
     CDS             5853..6302
                     /locus_tag="IV328_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938733.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            6302..6796
                     /locus_tag="IV328_002666"
     CDS             6302..6796
                     /locus_tag="IV328_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938734.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            6810..7241
                     /locus_tag="IV328_002667"
     CDS             6810..7241
                     /locus_tag="IV328_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938735.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            7243..7380
                     /locus_tag="IV328_002668"
     CDS             7243..7380
                     /locus_tag="IV328_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8938736.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            7418..>7662
                     /locus_tag="IV328_002669"
     CDS             7418..>7662
                     /locus_tag="IV328_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938737.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLV"
BASE COUNT         2772 a         1047 c         1614 g         2229 t
ORIGIN      
        1 tggattgata taaaatattt tgcaattcag aaatggattg aattgaagtt ttctattatt
       61 caaacttgga tcgatttaaa atataatgcc attactactt ggaataatat taaacagttt
      121 ttcaaagata cttggaaaaa tattaaagat acggcataca atacgtggat ttctataaaa
      181 aataccatga ttaatacatg gaataacatt aaggactctt tctggaatat tgttactgga
      241 attgttaatt ctgctgaaaa tgcatggaca aatctaaaaa atggtgtttc aaatgctatt
      301 aatcgggtaa aagaaatctt taattcgtta agggaagtca atttatttga aattggcaag
      361 aacatcattg atggacttat aaatggtgta aaagaaaagt ggaatgcttt gaaagatact
      421 atcaaaggta ttgcaggtaa tatcaaaaat tggattaaag gagctttaga tattcattct
      481 ccatcaagat ggatgcgtga tatggttggt aaaaatattg ttcaaggtat tatcgttggt
      541 attgataaag agcaaagtaa gttagatcaa accatgacaa atttagtaaa aacaccgtct
      601 gttcaacctg tagtaaatgg aataagtcac ctaccaatta cacaaacaaa gcaaaatata
      661 tctgctagtg aaacaaaaga aattcattta catttaaatg tttatggaga tttacctgat
      721 tcaatggttc ggaagattac cacaaaaatt aaaacagaat tgacaagaca aatgaaacga
      781 gatgctggtg cagtaggagg aacattatat gcaacttaag agaggacaat tttttattaa
      841 tcaacattat tcttctgaat ttaatgtgta tattcaaaac aggcctgctt ctgtttcagc
      901 tagtcgagta attgaattga gagagcgaga aggaaatgat tctatcatta ttgataaagg
      961 ctattacaaa aatgtgacta gaaagattga gtgttactat aaagcacctt caattgatat
     1021 ggtgcaggag tgggaagatc gaatcactga atggttagat atgtcgtctt atagtgattt
     1081 tattctttac tatgatgaac aatatattta tcaagctgta gtaatagaag gacctgaatt
     1141 taaaggaaca agaaaaacag gaaatattgt cccttttgaa tttacagtga gtattcgacc
     1201 atttaaggaa aattacaatg gtagatttac tattcgacaa acagaaactt ttgaaattta
     1261 taacccagag aagtattctt caaaaccgct tattaaattg agtggttctg gagatgcttc
     1321 tttttatatt aataaggata aatatgattt gaaatcgttg gacagagaat tatttataga
     1381 ttctaaatta gaagaggctt atcgaaaatt agatggtaat ttagaacatc aggatcaggt
     1441 caccttattt ttagattttc catttttata tcctggaaaa aatgaaatta aatggacgaa
     1501 aaatattcat tcatttgaaa taatgcctag gtggtggaga aaagtatgaa accaagaata
     1561 tatagtccta ctgaaacaga tttctcaacg aatggcttag gaattttaaa ggattgtaca
     1621 agatgtggaa tatatgaagt agctaatgga aaatatgaat tagagttgga ttatccttta
     1681 ggaactagat ttgatgaata ttttgaaaat gactatcaaa taaaagcaaa gccaaacgat
     1741 caagaagagt atcatatctt ttttattgat gataaagata tagatacttt tttaaataca
     1801 gtaactattt atgctcagag tcgtacaaat cgacttggaa gacgggtagt cactcttgtg
     1861 gaagtggact ctaaaactgg tcaagaagcg atatcaatta ttgaaactaa aatggataaa
     1921 aaatctgaca tacgacttta ttctgatatt acggctgttt ctagcacaat ctttgaagcg
     1981 agaaatgttt taaattgtat tgctggtgaa caaggatcat tgcttcaata ttggggcgga
     2041 gaaattaaac gtgaaccatt taagctttct ttgttaaagc gaagaggtcg caataatgtt
     2101 ggaacgattc ggtatggaaa agatatgtct ggtttgaagg tcaaattaga ttggacaggt
     2161 ataaaaacaa gaattattcc atatgctgat cctcaaagtg aagtaggtac gactaatcga
     2221 atttatggtt cgccagtaga tagtgcctat attaataact atcctgatgt ctatacagaa
     2281 catgttcagt ttacagaaga acaaggagta aaagacatta agagcttaaa taaaatagct
     2341 aaaaattact ttaaaacgat caatcctggc tgtgataagc ctaaagtttc tattacggtt
     2401 gaatttgata agttgactga tagtgaagaa gcgaaagaat ttgcgaagat tagaaattat
     2461 ggtctgtttg atacatttaa aatatatcat aaaaaatata atatttatct tgaatcaaag
     2521 gttagtggac tacaatacga ttctttatct gaaaaaactt tgaaattaga agcaggagac
     2581 attcaagttg ctttttatca gcaacaagct gtaactattc aggataaatt aaaagattat
     2641 gcaacgaata attatatgag tgattttaat gattatgttt cttcaatgat tacaggtcaa
     2701 ggcagtgcag gaggatatgt agttttatgg ccaaaagaaa aaccttccaa catctttatt
     2761 atggataatc cagatttaga aaaggcaaga gaagtactaa gaatgaataa aaatgggatt
     2821 gctttttcta agaaaggatg gcgggggcct tttaattcag cttggacatt agatagtata
     2881 tttaatgcca attttataca aactggatta atcaaagcag atatctttca aaattctttt
     2941 aacaagactg gagatgtatt gaaattagtt aatggactac tccaaatttg gaacaacaaa
     3001 aagaaaataa tggagttaac taaaaaagga atggaatttt ggaattctaa gggttcaatt
     3061 ggaacgattg gaacaactga ttctgttggt aatccttttc ctggggcttc tactccaacc
     3121 cctattgaag ataattcttt agttattcgt acaaatggag acggcaaata tattttgatt
     3181 tctcctaaag taggaaaagg attagtttta ttaggaaatg gtaaagcaat ttattttgga
     3241 gacttagatg tacaaggaaa acttacagtt aaaggaaaag aaataattcc tggacaaggt
     3301 ggtggtccat cgggaggtgg agaatctact ggtgggtatc ctagtgaatt aaaaacagat
     3361 gcagaaaaga gagcttggag aatttacgat attttgtgta ataacggatt tacgaaacaa
     3421 tctgcatgtg gaatattggg gaacattcaa caagaaacag gaggaacttt tgatcctgat
     3481 actgttcaaa taggtggacc agcatacgga ttagttcagt gggatggttc ttcatatcct
     3541 cttgttggtc cagcaacatg ggatggaaaa gtttatgttc aaaacttatt taatgctgca
     3601 ggtattaaag aaccaataac gagcttagat gcacaagttc gattgcttat ttggacattt
     3661 acaaatggac aatggatggg cgtagtacaa cctacgacgg ttgatggatt taaggcctgt
     3721 actgatccaa gacaagcagc atatgctttt gaacgaaact atgaacgtcc ggcagcgaca
     3781 catcctgaac gtcaagatta tgcagttaac tggtataaca aatttaaaga tttaaaacca
     3841 ggaggagcta ctggagaagc aggactaaaa catttagagt ctttgattgg acaacgaatt
     3901 ggcaatggtc agtgttatgg cttgtctgca gaatattcag gatatctggg tggttgtggc
     3961 atgggtgctg gaacaaaata tggtttaact catgtgatag gaaatacttc tgcagcatct
     4021 gatattggta ttgcctatga ttggtctgct gttggttgga aagtgattca aaaccctaga
     4081 tatgatcaat tagtagttgg tgcaattatt aattgggcaa gaggtggaca agtgggttca
     4141 tggtttgcag atggaactta tggacatact ggcgttatcc gaggcttagc taatggtcgt
     4201 atgcaaactt atgaacaaaa cacagagtta ggtatgattt gtggaaagtt agatcgccag
     4261 tattatagtg caagtgcaat ttcttccatt gtcataccac caaaataggg aggtgattta
     4321 atggcaaagt ggaatgtcat attaagtaca acagaaccct ataattacgt ggggatgatt
     4381 caagttcgac aaggcaataa gaatacagag gttatggaag cgactatagt tgaaaatggt
     4441 cttccctacg atttatcaga atgtaaggta tattttgaat cagttgtagg tgggaaatat
     4501 ccagtccaat tagaaacaaa aattgtggat gctaaaaaag ggaaaattaa ctatattttt
     4561 gataaatatt ccatgcagtg tttacatcga caaacagcca atttcattat atttaaagga
     4621 gaagacttga ttggaacaac tcaagacttc tcttattttg tcattaatgc tgtttcaaaa
     4681 acagaaggag aaatgggttc ttattggcaa tcaatcgaag atttaattgc ggatatgacg
     4741 gactttatta atgaaaataa aggcgatttt acggactgga tgaatgaaag gaaagaagaa
     4801 tttgaacgtt ggagacaaga acaggaacac agtttccaag attggagaga aggacaagaa
     4861 tcagactatt tggcatggtt tgaatcaatc aaggatattt taaagacgat tgatccaggt
     4921 ggagtcatgt tagccgaact aacggatgcg cgagtagata ttcaaggagt tcgtcatgcg
     4981 tctatttcag agcgtttgct ggcagatatg gactatttat atcagaaatt gcgagcaacg
     5041 cttttcacga ttgaatacgg ggagattgaa gtgactgata ttttgcagga tgatctcttt
     5101 tcagataatc atgaagtcga aaaagttgga actgtagcat tcccgattga agaaggagcc
     5161 ttgatcatcg caaccgttga tgatccaaag caaaatgtgt tcactcttga gaaagttggg
     5221 gtgatctaat ggctaaaact aaacgaatga tggaaacgga tgaaaaaacg ggtgtacaac
     5281 gccaattctt tccaatcaca catgcttccg cagttcttgg gttagaagaa ataatagcag
     5341 gagaagcaac agttttatct gttaatggaa aaattggagc cgtcgtcatt actaaagaag
     5401 atttgggatt agagaatgtt ctcacagaat taccctatgc aagtgaagaa gatgacggca
     5461 ttatcacggc agaaatgtat caaaaaattt taaacagtgg agaaggtgac tacgtgttgc
     5521 cagttgccac tatcgaccgt ttgggtggca taaaagttgg tgaattattg acgattgatg
     5581 agacagggaa agtctctgca gtcagacaat ctgatgtcaa tttttcgctg gagttaaaag
     5641 aaaaactcga ttcactgaaa aattatactg ctggagaaaa catcactatt gatgaagatg
     5701 gaacaattag ttctacaggt ggtagtggga ctggtggtgt caatcagtca tatgtagatc
     5761 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     5821 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
     5881 gcaatgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaacgg
     5941 tgaagggaga taaaggcgat cctggccaag cagcaacaat taccatagga accgtttcta
     6001 gtggttcaac cgcttctgtt acgaatgtag gaacatcatc tgcagctaga tttaattttg
     6061 ttttaccaaa gggagataaa ggtgatcctg gaataaatgc cacaactaca gctgtagcta
     6121 caactacagc aaatgggttg atgtcgtcca cagataaaac taagttggat ggaattgcag
     6181 ctggagcaca gaaaaatcca ggtaatgcta caactacgac agcaggttta atgtcagcaa
     6241 ctgacaaagc taaactcgat ggattagcca atattacatt tgagaaagtg gggacggttt
     6301 aatggctgat attgtgcaat taaaagaaga cggagttgct aaatacttaa aaacgcatgt
     6361 agaagctatt gatggtaaag aagcattggt gcagacagat ggagaccaag ccattgcagg
     6421 acataagaat ttttcaggtt ctgtaacaat caataataaa cgtgttttga cgactgatga
     6481 taacagatat gaagtggtaa atctggttgt cactaacggt aatacaggga cagcaaagct
     6541 ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa aaagtagtgg
     6601 cgggaatgat tcggttattt taactgttcc agaaggctat cggacaccaa ttagttttga
     6661 acaactggtt gggtcaatag accgttctac tttgaacagt gctcaactat ctattggcgc
     6721 agacggagcc attaaatgga gaagaaattc aagttatgga tcagcttatt catttgttat
     6781 cacttattca atttaaggga ggaaatctaa tgaaagtagt ttacaaatca atcaagcctt
     6841 acggattcga gcaaatcatt ttgaacaatc aagaaaatat ccctgaaaac tgtacagaga
     6901 ttaaaccacc agttcctaac tggagaccaa gatttgattt tgataaaaaa cagtgggttg
     6961 agttagctac tgaagaagag aaaaaaggga atgctgttga tagcgtagat gaattggcta
     7021 atattaaggc attatacgaa acgctgaagg cagaaaatga tgaattgaaa cagctaaatt
     7081 ctaaagcaat gctaaacaat gtagcaatca agcaagaaaa tgtcttattg aaagaaaagt
     7141 cagaaagttt agcgcagttg aattcaaaaa cgatgcttgc ttctgtacaa aataccaagg
     7201 aaatcgaaga aatcaaaaaa caattacaag gtgggaagta acatgtattc atatgaagat
     7261 atcaaactaa tgtatgactg gggctgtttc actaacgaac aagtaatggt tttcgttccg
     7321 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
     7381 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
     7441 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
     7501 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
     7561 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
     7621 gaaacacgat gaaagattag atacacatac agaaaaattg gt
//
LOCUS       DACDKJ010000096         7630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDKJ010000096 DACDKJ010000000
VERSION     DACDKJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.098"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(60..788)
                     /locus_tag="IV328_002670"
     CDS             complement(60..788)
                     /locus_tag="IV328_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8938738.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(801..2081)
                     /locus_tag="IV328_002671"
     CDS             complement(801..2081)
                     /locus_tag="IV328_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8938739.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2222..2824)
                     /locus_tag="IV328_002672"
     CDS             complement(2222..2824)
                     /locus_tag="IV328_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8938740.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3069..3275
                     /locus_tag="IV328_002673"
     CDS             3069..3275
                     /locus_tag="IV328_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938741.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3587..4306
                     /locus_tag="IV328_002674"
     CDS             3587..4306
                     /locus_tag="IV328_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8938742.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4387..5070)
                     /locus_tag="IV328_002675"
     CDS             complement(4387..5070)
                     /locus_tag="IV328_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8938743.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5288..7474
                     /locus_tag="IV328_002676"
     CDS             5288..7474
                     /locus_tag="IV328_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8938744.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2733 a         1284 c         1438 g         2175 t
ORIGIN      
        1 aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt
       61 tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac
      121 gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat
      181 ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat
      241 tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa
      301 aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag
      361 atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg
      421 acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg
      481 ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa
      541 ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt
      601 tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt
      661 tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta
      721 tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg
      781 ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg
      841 ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata
      901 aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac
      961 tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc
     1021 ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca
     1081 ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg
     1141 attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt
     1201 aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc
     1261 ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat
     1321 ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca
     1381 tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc
     1441 atcctgactt gttgctctaa taactgatca gctaatttct tattagagat tttccaataa
     1501 taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct
     1561 aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat
     1621 aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct
     1681 aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga
     1741 aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgtcg
     1801 atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc
     1861 aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt
     1921 agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga
     1981 atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt
     2041 gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga
     2101 tttttaccaa attatagcac aattagcgta ttattttttt aatttttcag ggattttgaa
     2161 ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg
     2221 tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag
     2281 gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag
     2341 ctgagaaaaa taaagtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata
     2401 atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa
     2461 gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt
     2521 gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa
     2581 cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa
     2641 aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta
     2701 gttttccaag attattcaat aaaacttcta aggcaaattt tacttggaca tacaccatat
     2761 cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt
     2821 tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg
     2881 tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt
     2941 tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag
     3001 tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag
     3061 ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg
     3121 atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga
     3181 tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt
     3241 tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata
     3301 aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct
     3361 tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt
     3421 tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac
     3481 ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc
     3541 tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt
     3601 gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca
     3661 agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt
     3721 ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt
     3781 tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga
     3841 agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa
     3901 gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa
     3961 gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa
     4021 aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa
     4081 agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt
     4141 gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca
     4201 agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta
     4261 cactacaaat aacccgaaag caaagggaat ctttacaaat aaataatata aaaactaagc
     4321 aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca
     4381 ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt
     4441 gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg
     4501 atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg
     4561 gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc
     4621 tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc
     4681 aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg
     4741 ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa
     4801 caagcgccag aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag
     4861 aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc
     4921 gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc
     4981 atattggata taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat
     5041 tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg
     5101 tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc
     5161 atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac
     5221 gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat
     5281 caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa
     5341 caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga
     5401 agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga
     5461 aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga
     5521 acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg
     5581 gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt
     5641 agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa
     5701 acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat
     5761 cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat
     5821 acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat
     5881 acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt
     5941 agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa
     6001 atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct
     6061 cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca
     6121 aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga
     6181 ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg
     6241 gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta
     6301 tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa
     6361 aactcttttc ttattgacta attatcgttc tcgggcagct attgtccaag gagcaaatag
     6421 attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga
     6481 agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat
     6541 caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata
     6601 ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt
     6661 tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt
     6721 ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta
     6781 ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca
     6841 atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc
     6901 caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga
     6961 ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa
     7021 ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt
     7081 cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg
     7141 cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag
     7201 taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta
     7261 taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtgac
     7321 gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct
     7381 gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa
     7441 agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg
     7501 tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt
     7561 cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga
     7621 caagaatttt
//
LOCUS       DACDKJ010000097         7620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDKJ010000097 DACDKJ010000000
VERSION     DACDKJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.099"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..578)
                     /locus_tag="IV328_002677"
     CDS             complement(<1..578)
                     /locus_tag="IV328_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8938745.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQT"
     gene            complement(578..1714)
                     /locus_tag="IV328_002678"
     CDS             complement(578..1714)
                     /locus_tag="IV328_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8938746.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1746..3196)
                     /locus_tag="IV328_002679"
                     /pseudo
     CDS             complement(1746..3196)
                     /locus_tag="IV328_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            complement(3240..4004)
                     /locus_tag="IV328_002680"
     CDS             complement(3240..4004)
                     /locus_tag="IV328_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8938747.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4032..4730)
                     /locus_tag="IV328_002681"
     CDS             complement(4032..4730)
                     /locus_tag="IV328_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8938748.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4742..5521)
                     /locus_tag="IV328_002682"
     CDS             complement(4742..5521)
                     /locus_tag="IV328_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8938749.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5537..6481)
                     /locus_tag="IV328_002683"
     CDS             complement(5537..6481)
                     /locus_tag="IV328_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8938750.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6527..7306)
                     /locus_tag="IV328_002684"
     CDS             complement(6527..7306)
                     /locus_tag="IV328_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938751.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2146 a         1608 c         1169 g         2697 t
ORIGIN      
        1 aacgtctgac ttgaaattcc taatttcatt cgtacatcat ttctaatact ttcatcataa
       61 taaaattttt tagcatccac tgcatttgga atcaaagtga atttatcatt tcgaaacaaa
      121 aaatcacctg ctacttttcc acatgaaaat tcatgtgtca aaacagacgt taacttacat
      181 cgataatatt gctttaaaag atatttaaaa tctctatcta tcgatgtatt atggctatgt
      241 gctatccgta tcggaatccc agcttttttt gcagctttta aaggtaaata actcatcgca
      301 tcaatatgag aatgaacaat ctcatattct ggatgatcct taaaaaattg ttccatccat
      361 ttaaaatatt taggataatt ttgtggataa agtctcggtg cataatagac ctttcctcca
      421 agacttatta tttcctcatc ataatcactt ttatatggac gatgtgtcaa aaaatcaaac
      481 tgaattttag ttcgatctat attcctataa tagttcatta gcatcgtctc caaccctgca
      541 cgatgcatat cattcacaca ctgtaatatt cgaatcatta tatatttccc ctatacaaat
      601 ttaatgtttc ttgtacgatt ttatcccaat tatatttact acaaataaac tcagtagctt
      661 gattcttcaa aactttaacc atttccgatt gattgcatgc ctcttctaat cttatcttta
      721 aatcacttac atccgatttc ttaaaaatca tagccttgtc ttctactact tccgtacact
      781 ctgagatatt tgaaactata caacagttcc cataactcat tgcttctaac aaacttaatg
      841 gcattccttc taaatcactt ggaagtgtat agatatacgc attactatat aattcttcta
      901 attcctgtcc ttgtacaaat ccagtaaata taattcgctc atcacctttt gctaattcct
      961 ttagttcatt tgcaaattca tctgtatcag agcttcctcc ggctattata agttttttat
     1021 ctgtctgaac atctttaaat gcctcaatta gataccgaat tcctttttcg ggaaccaatc
     1081 gccccaaaaa caaaatataa gaatcttttg ttaatccata cttttctgta atcaatcctg
     1141 ctgattttat ttcaattttt ttaacgccat ttggtataaa ccttgttttt cttccatata
     1201 tatcttcaaa ataattttgg acgctttcac taagtacaat tatttcatct gcaaatttta
     1261 cagcacactt ttcccctaac atgatgtatg tactagcaag cttgttccat ttttctcttt
     1321 gatgatctag accatgaatt gttgcaacac atcttttccc aaaaagtttt ggcaaccaca
     1381 acatagcaca tggtccttct gcatgaaaat gaacaacatc atattttccg aatgctgctc
     1441 taattccacc aaatactgaa gaactcattg ctgcaattcc ttttatattt aatgtaaaaa
     1501 tagtctttaa ttttacacct ttatattctt tatttttatg ctggtcaaat tcctttccgc
     1561 taacatgatg accacttcga ttatagcaag tcacttcatg cccaagtgca accattcgcg
     1621 ttgccaattc ttctaccact atctcaattc caccttctct tgatggaatt cttttatgac
     1681 ctagcataca gatttttaat ttttttcttg ccatcacgtt ttactccttc tatagtatct
     1741 ttatcttaca tggaaccgtt tctatgaagc acagttttga ctgtcctaaa caaaattcta
     1801 aaatctaatc ccatactcca attcctgata tattctgcat caagttttac aacctcttcg
     1861 aagtctgtga tatcacttct accactcacc tgccacattc cagtaatccc aggtttcatc
     1921 gcaattctcg cacgatgatg aaaatcatac ttctcatatt ctgacttcaa gcaaggtcta
     1981 aaccccacca ggctcatttt tcccattagg acattataaa attgtggaaa ctcatccaat
     2041 gatgtagatc gaataaattg tccaattcct gtcttctttg ttccatcagg aagtatttta
     2101 ttgccaatga ttcttgggtc aaaatccatc ttaaacatga gattattact catcttattt
     2161 tctttcatca actctgcttt gcgttcttct gcatccatat acatactacg gaacttatac
     2221 atcttaaatg tcttaccatt ttgaccaata cgttcttgtg caaagaaaat cggccctggt
     2281 gatttgatgt aaataactgg tgctacgaag ataaaaataa cacctgttgc aatacatcca
     2341 ataattccag caagaatgtc aatcgttctc tttataaatg cttgcctcat cgtcataaca
     2401 ttaatactag atgtaataac agtataatca ccaactcggc caataaattg ttttccacgt
     2461 gtactatcag acacttttgt aagattcata tgtacagcaa gtcccatttc cgaacatctg
     2521 gcgattaatt ctttgggata tggaacactt tcgtcaacat tgatgaatac ttcatctacc
     2581 caattctgta aagatattcc gctgcattat cagcatttgc aactactggg acacctccga
     2641 tggtagttcc atacatatct ctgtctacta caatcacacc actaagttta tacatgccat
     2701 aattcttctc tttaagatta cgaactacat tttttgcaat cttttgagat gttacaataa
     2761 ctaacgatac atcttcaccg tttttcatgc ggtgatgcaa caaccctttc caaagtatac
     2821 gaattatata tgttaataat ccatacacca cccccattat aaataaagaa actctggaat
     2881 aatcatttcc atcctgcatt gtaataaggt ataaactgca cacaagtaac agcataaaag
     2941 attgctttat agaagctaca gcctctttat aatatcctct ttttaaaaca tttttatatg
     3001 cctcaaataa aaatataatc gtaagattct caagttctat aaaaattgcc atgtggcgat
     3061 aaataggaat cacatacgga ttccagtcac cctgtcgcat tacatatgat atataaaaag
     3121 caagctggac acaaaataag tctaataaaa taaaatcaat atgtttagac caaccacttg
     3181 agttttttct atacatctct cactctctcc tcatttcctt tttatctacg aataaagaac
     3241 taaaataaac caaacttctt cttttttacc tcttcataga ttggataatt gatctcatcc
     3301 ccgttgatca gatcctttgc gacttgtttc atctgtacta ctttttcctt accaaactcc
     3361 ttggcaatct gctcatagca ctcttttaag taaaaggtgc gcttagagac cccgtgtgca
     3421 tcagaagcag ccatctgcac gagattatgt ttgaccattg ttttggctgt tttctggata
     3481 tctttgccaa atacaccaac ataacttggc gcagtaagtt gggccaaaca tcccatatct
     3541 aggaacggaa gcaaatgatt tgggtcttct cgaaaatgcg cgttgcgttc tggatggacg
     3601 atcactggta tctttcctag ttctaacaac tcaaaaaaaa gccgttctgc atataacggc
     3661 acttccagtg tcggaaattc gatcagaaga tatgtatcat ctaaatctgt aaataaaatt
     3721 tcatctcggt ggatgtcttc gatgagcgtt ccggtaatac gtacttcttg tccttccaat
     3781 aacgtcaaag gcaactgtct tttctctaat tcggcttgta acgaagcgac tagagaaatg
     3841 acttgtgatt tttcattgct gtattttcca ttattgtggt gtggtgtgca taaaatatgc
     3901 gtaatgcctt gctgaatcgc tttttcagcc attgcaatac ttgcttctaa gttttctgcc
     3961 ccgtcatcga ttcctggtag gatatggcaa tgtagatcga tcatttctct tgtctcctct
     4021 cttcttattc cttaatttcc gtagtaataa ccagcatctt tgctgtgttc tgctccgttg
     4081 tatacgacac ctaagactcg tgcttgtacc atatctaaca gttcttttgc tttagtcaat
     4141 gattcttttc gtgacgtatt ttcccgtacg accaataagg tgccatccgt ttttgaagac
     4201 atgatctgtg catctgttac ggcaacgact ggcggcatgt cgaagatcac tacatcaaat
     4261 agttgtcttg cttctgctaa aatctgatcc attcttggcg aacttagtag ttctgatgga
     4321 tttggtggtt taggtccact tggcagaatc gacaaattgt cgactggtgt tcgttggatc
     4381 tcatctgcta cacttcctga tgaactaaga acagtactta atccgcttgc gttgttcaac
     4441 ttgaacgttt tatagatgac gggcttacgt aagtctgcat cgactagcag cacccgttga
     4501 ccagatttgg caaaaacaac ggcaatgttt gctgcagtgg tcgatttccc ttctccaggt
     4561 cctgatgaag tcacaacaat cgttttgatt tgctgaccag ctgcggaggc aaactggata
     4621 ttcgtccgaa tcgtacgata ttgttcagcg attggtgaag agggattggt gagtgtgacc
     4681 aaactgactg catgagtttg ggctgctttt tgtttctgtg ttcgtgccat tttctttcct
     4741 cctagactct tgagcggctt ctgcgtgatg gcgctgtttt atcgtttgta gcttttggtt
     4801 gtgtctgagc tggtcctttt ttacggatac cagcattcaa ttctttcgct gacatttctg
     4861 gaactgttcc taagattggg aatcctaatg tatctgtcac atatttttca tctttcactg
     4921 tccgatcgag taattccaat aagaatgcca gacctacacc taccatcatt cctaacatta
     4981 agccagtcat taaattcaat ttattatttg gcgaaacagg tgtcgtattg gcaactgcac
     5041 ctgaaatgat cgagatttta tcaacattca tcacatcttt tgcgttctct tggaaaactt
     5101 gtgaagttgt gttagcgatc tgttgagcag ccactgcatt cgtacttatt gcctgaatcg
     5161 agaacatttg tgaattttct gcctgattaa ctgagatggc atctttgatt tgttcagcag
     5221 tcattttcac attgtcttct gtttctaaac gatctttgac ttcattgagc actaaatcac
     5281 tgacgatcat atctttatac gtattgatca tttgcaggtt cgtattgaca tcattgacat
     5341 ttgcagtgtc tgattgtggc agtgtcacga tcaattgtgc ttgtgaactg tattttggtg
     5401 tcaagatgaa aaatgttacg attcctgaga tggccaagcc agcaaacatt gagatgatga
     5461 ttattaccaa gtgtttgcgt aatgtttcaa aaatttcttt caagctgatt gtctcttcca
     5521 ttttttctcc actttcctat ttattctcta gattcatttg atcttttaat tcttttttca
     5581 cacgatcaag ttcttgatca ctgacttttt gataggagat attatcttgc atgaagcctt
     5641 ctccttgcag ctgatcttgc ttaatcgtac caaatgcctc ccgataatct aaagcaattt
     5701 ttttcatttc atcaaagttc atgtctgttt tcatattcga ctctagagca ttcaaaacgg
     5761 attgatagtt gctgacaccg tttagactca atactttttt cgctacgcct tctacgatct
     5821 ttctttggcg cccttgtcta ccgtaatcac catttgggtc ttcataacgc atacgagaat
     5881 aagataatgc ctgatccccg ttcaatgtaa ttttaccgat tggaaagtca tagccatctt
     5941 gtgagaaagt gaggtcatta ttgacttcga taccaccaac tgcatcaacg agttccttta
     6001 gccctttcat attgatagag acatagtgat cgattgggat gtctacatat ttttgtacgg
     6061 tatccatcgc catggcagct ccgccgaatg cgtaagcgtg gttgatcttg tcttttgtgt
     6121 cacgacctac gatgtcgaca tacgtatcac gaggaatgct gacgatcgtt gtctgtttgt
     6181 cttcaggact gactgtggca accatcatcg tatctgatct tccttgatcg acccgaccta
     6241 aatctccagt atcaatcccc atcaatagaa cagagaaaga ttcttgtttg tccagatcaa
     6301 ctggtttcgt tcgttctcct tgggttcgtt ccactgattc gtaagttttc tcaatagaat
     6361 gtgagagatc cagatataat tttgctccta ctcctacaat tgctaatact aatacgataa
     6421 gaactgctaa aataatcaga aatatttttt tccctcttga ttttcttctt cgtcttgcca
     6481 tagtttcctc cgtttgctaa ccttgcttga atcaacgttt taactgtcaa tagcttatat
     6541 tgctatagcc catttgctca gcaacagttt gtgcttttgt tgcgtatgct gcgcttattt
     6601 tttcattccg gatctgctct atttgactga gcaccgtcaa atattgatcg taagtagcgg
     6661 tggcagtcac ttgaccatcc tttaacatcg tatcgaataa ctgatctagt tgcgtgactt
     6721 gcgcgatctg aatatcagcg aatcctagat attgcataac aaccgttttc caaggactat
     6781 ctttaaaaaa gctcatattt tcacgtacac gagcgacatc tgcacttgtc accttttctt
     6841 tgatgatgac atcgtctaca gcttgctgcc agtttgatac atttttagta aataaatcgt
     6901 tgacagctgt ctggatcttc agttttgatt ctgcactatc taactgtgtc acgattgctt
     6961 tcttctcatc ttggattgca acagcttctt taggcaattc tgattctttg atgtcgtaat
     7021 catctgctga aagtttgaca gaggatagct tatttgacaa acttgtgatt gtttcttctg
     7081 ttatttcagg gtttagaaaa attttttctt ttgtactata cagcttgtct acttcttttg
     7141 ataaagaagt caaggtgtct ttttgattgg tgatggcaga ttctgcataa ttgatccgtt
     7201 gctgatgttg ccgctgatta ttgatatggt atccaaacaa acagatcgca atagcacctg
     7261 cacctaccgc cacagcagtc aatgacttga cgaatggctt ttccatagat tcactcctaa
     7321 gtattgatga tagataaatt aagatataat tcacttacat aaaaatgaca gctttttatt
     7381 taaaaaaaca taattaaact aatatattca cagaaatatt tgtaaacaag ttttccctat
     7441 ataatcatac cacaaactgt tttcttgact agggcaattt acgatttaaa agtaaataaa
     7501 acattaataa gataaaaatt aatcgatttc atttttattt ccataatttt cataggtgtt
     7561 tttaaaagtt acacaaaaaa tagaataaca aaaaaattaa cattgaatta ataatagtta
//
LOCUS       DACDKJ010000098         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDKJ010000098 DACDKJ010000000
VERSION     DACDKJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.100"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IV328_002685"
     CDS             complement(138..428)
                     /locus_tag="IV328_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938752.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IV328_002686"
     CDS             complement(546..806)
                     /locus_tag="IV328_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938753.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IV328_002687"
     CDS             complement(943..1356)
                     /locus_tag="IV328_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8938754.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IV328_002688"
     CDS             complement(1812..2297)
                     /locus_tag="IV328_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938755.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IV328_002689"
     CDS             complement(2436..2651)
                     /locus_tag="IV328_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8938756.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IV328_002690"
     CDS             2875..3675
                     /locus_tag="IV328_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8938757.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IV328_002691"
     CDS             3694..5562
                     /locus_tag="IV328_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8938758.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IV328_002692"
     CDS             5555..6046
                     /locus_tag="IV328_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8938759.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IV328_002693"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IV328_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8938760.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IV328_002694"
     CDS             6606..>7600
                     /locus_tag="IV328_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8938761.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACDKJ010000099         7264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDKJ010000099 DACDKJ010000000
VERSION     DACDKJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.102"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..597
                     /locus_tag="IV328_002695"
     CDS             <1..597
                     /locus_tag="IV328_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938762.1"
                     /translation="NINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKW
                     AKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKRED
                     FIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYIN
                     SLSEPLKCRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            780..3455
                     /locus_tag="IV328_002696"
     CDS             780..3455
                     /locus_tag="IV328_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8938763.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3637..3876
                     /locus_tag="IV328_002697"
     CDS             3637..3876
                     /locus_tag="IV328_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938764.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3892..5265
                     /locus_tag="IV328_002698"
     CDS             3892..5265
                     /locus_tag="IV328_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8938765.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5269..6903
                     /locus_tag="IV328_002699"
     CDS             5269..6903
                     /locus_tag="IV328_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8938766.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7066..>7264
                     /locus_tag="IV328_002700"
     CDS             7066..>7264
                     /locus_tag="IV328_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938767.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2592 a         1132 c         1445 g         2095 t
ORIGIN      
        1 aatattaata aaacagtgga aaagaatgga gtttataaag gtgattttca ttttcaaaat
       61 gctgagacta ttgaacgaga acatcattat ttcgatttat tagtgaatac agtggtagaa
      121 ttagcgaaat gggcaaagtc aaacgaaact tatagtcaag tacttaaaag aattatgagt
      181 caagaaactt catttatcta tgagttaagc aagtcaaatt tgaaatatac ttataatgaa
      241 gatgaacttg aaattttaga taatccagaa aattcacaat ggattaaaga gttgaagaga
      301 gaagacttta ttgagacact aagaattcta aaatcgttat ttgatttgaa attagccaaa
      361 gctaccatca tgggaattga aagagattca tattattggg aggaaattgg actatatgct
      421 ccccagattg aaggtgaaga taaagagaaa aggatagaca acattattaa ctatattaat
      481 agtttaagtg aacctttaaa atgtagagga cttgatttta taggaacatt aggatggaca
      541 gaaacatact atgagaaaat atataaagaa ataattgaac aatattttcc taaataattt
      601 aataaggatt gatttatcgt aaaagaaaaa agttgtgatt tccaaaatca caactttttt
      661 cttttgtcaa gattttgagt gggatatatt tttgtagaac tgaagatcat gaaattatga
      721 gaggttggag gtaatgtagt catacaaata aaaatttttt agaaagtgag tggtaattaa
      781 tgaaaatgaa tcacacttca attcagaagc atttacaact gttaaataca ctggggattt
      841 cttatgttaa tttgagaact ttttctacat taaacaagaa aaccgtttct catatttttt
      901 ctactgcaga ttctcaagca attattaact atttagaaaa acagaaattg tctgaaggta
      961 cagtaaacat aacttataat cctattaaac aagaggtgat tacacaaaaa aagcattctg
     1021 tgagagacaa tgatattgaa aaaattcggt ttgtatttat tgatattgac cctaaaagga
     1081 aagagggaag tgcaacagat agcgaaaaga aaaaggcaga aaatgttatg gaacaagtag
     1141 aacgttattt gaaagaaaag agaatagaat catttgtaaa agttgatagt gggaatggct
     1201 accatatttt tcttcccata aatgaacaac caaataatca cgagacgatt cttactattc
     1261 agaattttct tcaattactt caccgaagat ttggagtaga agatgaagtt gatattgata
     1321 cttctgttta taatccttca agattatgca aattagttgg tacaccagcg gtcaaaggaa
     1381 agtccacaaa agagcgtccg catcgtttat ctagtttcct ttctattccc gataacatag
     1441 atagaagtaa ttgttttaaa attcttgaac agatagttga agaaaattcc atgatgtcaa
     1501 cctctagagt aaaaagccat ccatctttta gagaagtgtt accttttatt caagcagact
     1561 ataaacaatg gttagctcac tatcctgaat taacttatac cataaaagag ggcgatcata
     1621 aaggagttac cttacttata tttgacgaat gtccattaag aaaacacacg aataattcta
     1681 atggttcttg tttgtctgtt acggctgata ataagattcg atttcattgt ctgcatgctt
     1741 cggataaaaa taaagatatt caagactttg taaaaaaata tccgttgcca aaacctgtaa
     1801 atccttctgt agaatcatta gaatccaatc aagaatatta ttttgaccag tttaaactga
     1861 atcaagaagg agtatttttg gtgaaagaag gtcaagaaga aaaagttgct tgtccgatgt
     1921 ttattaagca aatacgcaaa aatatcgaac aaaaagtcat tacacttgtt cttgcctatt
     1981 atgtcgatca ttgttgggat gaaatagaaa tagaaggtgg atgtttacaa actaataatt
     2041 ttaaacaatt agcaaaacat ggcttaatca ttaaagctag aagagaagct gaagtcgttg
     2101 actttttaca gattcaacgg gagaatgctc caatcttttt tgaacatgaa tctcttggct
     2161 ggaaaaaaga aaatgaagca atgatttttc agttacaaga ttcttttgga cgtgaaattc
     2221 cttctgtatt aactaaaaac tcatgctacc aattaacaac taatggtaca gaagcggaat
     2281 ttgacgcatt ggttcataag tatgttttag gaacgaactt agaacttgct tggtcgattg
     2341 gtattgcttc tattgtgtta ggctatttaa acgttacgaa ggctaatact tttttgagtt
     2401 tactaattaa cctacagggc aagagcacaa cgggtaaaac cactacgtta cactttattg
     2461 cctctttatt tggaaatcca actacggttc ttagaaatat gaatgctacc aataaagcca
     2521 ttataaagct agcatcaaac aatcagggag gtgttccact aatactagat gagttaggag
     2581 cagcaactca aaataacttt agctcgttga tctatcaatt agcttcagga gaagaaaggc
     2641 taagattaga ttctaattgt cagcttatgc cacaagagac tttttctgtt ttgaccctta
     2701 cctcttctga agagcgtttg gaaaattatt tcaatgagaa attaactggt ttgaaagttc
     2761 gatatatcga attttctgat atcaagttta caaaggatgc gaatcacgca gagaaagtta
     2821 aaagtctttc ttctaatact tttggttggg gtataaagtt atttcttttg aaactctttg
     2881 aacaagggat tacaaaaatt gaagcaactt ttgtcgaagc tcaaaaaaaa ctatgggctg
     2941 ttttacctga acattctttg aagcaacgca ttggtaataa tctagctttg atttatagtg
     3001 gagccctcct aattcaagat atactgggct atcctttacg attagagcat atacagacgg
     3061 tgcttattaa ttcgtacgaa cagttaattg tagatactag tttaggaagt gagcaaacgg
     3121 tatttgaccg agtgaaaaca ttgttggtga tgaatagcca taaattttta acaaacagtg
     3181 acaacaaaca tacgcccagt acgatttggg gaaaagtatc aattcaaaaa aatggggata
     3241 ttaaggtaaa tatttttcct atcccgtttg aggacttgtt aaggcgagaa tttcaagtaa
     3301 aggatctaac ctatcttttt aggcaattgc ttgattcggg taatatgtgt gcagaaaaag
     3361 ggagaagaac gaaacgtatt cacatgaatc gtcaagttct tccaacatac gagattctat
     3421 taccttcttc actcaaatcg tattttcgat tgtaatttag aataaaccaa agtatgaaag
     3481 tgaggcacca tgttatggaa atatttctta ttgatttagc aaaacaactg attacttatc
     3541 tgtatgatag cgagcaaacc caaacggaag taaatgaagc ttgatctatg cttcatttac
     3601 ttcattcaat caaacatgaa agaaaggaaa attctaatga ctaaaaataa aatgacacta
     3661 aaagcagagg ttcttctgta tattcaagaa catttttcaa atcaagcctt ttttacaaaa
     3721 ccaatttact tggatttcga aataagaggg gtatctgctg gatctatcgg gggtactctt
     3781 caagcgttga agaacgaagg atatttagaa aatcactttg tgcaacgttc gttcaataga
     3841 agagtcgtga aaaaatggta tttagttcat tcatagagag gggaatcaag catgcgtgtg
     3901 gcttgttatg atcgaacgtc tgtttatgaa gaagcgaaaa ttagtaatga cgctcaatat
     3961 gatgcagtga aagagtatgt tttagagaaa tatgaacaaa ttccccaacg attttcagac
     4021 aaaggagaat caggggaaaa agcggagcgt ccagcttttc gacagcttag agcttgggtt
     4081 tgtgcgtcag aagaagaggg ggtgatctat gtttggcgtt atgatcgatt ggcacgtcaa
     4141 gccaaaatag ccttagaatt tattgagttg tgccaacaac atcaggttca agtgtattcg
     4201 atttctgaac ctcttcctgc ttctttctca cacgcgtcaa cgaataaact atttgtgcaa
     4261 ttattgttca cttttgcaga acaacaacga aacgtcatta ttgagaatgt tcgtgccgga
     4321 ctttcctata aaaagcaaca acggcaatat ttgtcttctg cggtaccata tgggtatcgt
     4381 ttagtgaatg ggaaaatcca acaagaagta cacgaagcaa ggattgttcg ctatattttt
     4441 caactttatt tgaccaaaaa atatggctac aaaaagcttt gtcaacgatt gacgcaacaa
     4501 aagcttttct tccgcgaacg tccttttcag ccctatcata tttatagtat cctaaaaaat
     4561 ccattgtact atggcgaaat caaaggagga tctttgggga agtatctagg aacctttgaa
     4621 ccgattctct cgaaagcaac ctttttacaa gcccaagaga ttcgccaatc aaggtgtaca
     4681 gctaaaaaag atacgtatct gtacttatta cgtcaaaaaa ttcggtgtcc cttctgtggt
     4741 cgtcatctga gttcgaagta tcaatggaat acaaagaaga cgaagacgct tcattattat
     4801 cattgtaccg accgaagctg ccaaggaatt tttcttcggg cagaaagggt tgagtcagaa
     4861 gtattggaaa agatacaggc tttttttcaa tacgaaggcg tctatcaagc cttgattgcc
     4921 caattaacgc aacaattagc cgaaaagcga aaagatgaaa aacgcaaagt aatccaacaa
     4981 aagaaacaag aagcacaatg gattaaggca tttgaagagg gcaattgttc actggaagaa
     5041 ctcaagcaac aactgcaagc tcttcaaaag ccgatgtcta cttcttcgtt tctttcactc
     5101 caacagtgtg aagcaaaagt gaaagaatgg cttcaactaa aagacctctc tagtcgacaa
     5161 ttattatgtg actatgtttg tcggattgag gtaacaaagg ataaaacagt ttgtgggatt
     5221 tacttccgac aactgacaca aaatatttta gaaaggaacg aatgatctat ggaaatgata
     5281 caaaccaaac gagtcgcatt gtatattcgc gtgagtacac aagaacaagc tttagaagga
     5341 catagtattc aagcacaaga acaaaatgga cgacagtttg ccgaacggat gggctatgaa
     5401 gtggtagaag tttatgccga tgaaggaatc agtgggaaat ccacaaaaaa tcggttcgcc
     5461 tatcaacgaa tgatgaacga tgctcggaac catcgttttg atctactagt gatttggaaa
     5521 ttgacccgtc tcggacgcaa tatgttagat gtcttacaaa cagtcgagga attattgcga
     5581 tatgacgtag gattacattc gatctcggaa caatttgacg tgaccacttc gacaggtaaa
     5641 ttgatgttac aactccttgg gagtttcggt gaatttgaac gcaaccaaat tgcggagaat
     5701 gttcaaatgt caatgcaaag cttagtgaaa gataaaaagc gttatgcagg aggccgacgt
     5761 ttaggctatg tcagtggtat tgacacgaat ggtcaaaaac aattggtcat tgaaccagaa
     5821 gaggcgaaaa ttgttcgtct gatctatgca aaatatttag gcggagcagg gtatcgagcg
     5881 attgccaatt ttttgaatcg tcaaggatat caaacagtca aaaagaatgc tttttctacc
     5941 acggcagtca aagatatctt acgcaataaa atttatgcag ggtatttaga atatgcgcgc
     6001 tacatcaatt gggaaaccaa acgacggaag gggaagaatc cgagaccgat tttagtgaaa
     6061 ggaacccatg aaccgattat ttcggaagaa gattatcaaa aagtacaaga acgccttgct
     6121 ttagaaggaa aacaacccaa atggaaccat acaggtgaaa atgtattgac aggcttgtta
     6181 cgttgtccag aatgtggcgc accgatggca gcgagtaatg tcacaaatac cctaaaggat
     6241 ggcacaaaga aacggattcg atattattct tgttcagtct ttcggaataa aggagcaagt
     6301 gtgtgtcatg ccaattcgat tcgtgccgaa caagcggaaa catttgtcgc cgaccgatta
     6361 aaggagatcg ttcagctacc agaagttctt ccccgattgg tggcagcgtt aaatgaagaa
     6421 atagtgcgac aaagccaacc attggaacaa gaactcgtgg tgttactgga acgaaaggaa
     6481 gaattgaaga ctaaaattga aaaatgggaa gctgctttgg aagatagtcc tgaattgttt
     6541 ccgatgttaa aagaccgctt agacgaattg actgaaaaac gacgacagtt gcacattcga
     6601 gaaaatgaaa tcttagggat ctttcaacaa ggagagccga ttcaagtcaa agatgtccaa
     6661 cggatattga ctaatttgga tcgttttctt gcccagagtg agaagaagca aatcaaagcc
     6721 ttgtatcgta cctttataga gaaaataacc tttgatccta accataagga gatgttggaa
     6781 attacgatga aattcacgca tgccgtggta caacaattga atgagcaata tcaaatagcc
     6841 gtatccaaaa caaaggatac ggctattttt gtgttaaaaa caccctttac gttaacgatc
     6901 tagtctaggt tatatcaaac aacgttagag gggcgctaac gcttctctag cgatttgaaa
     6961 tcgttagaga agtagttagg gaggtcctta tcataaatag ggatatttat taatgaatat
     7021 ttgaaatata attgtatata agaattaaaa agagtgggga tagatatgaa aaagatgggt
     7081 aagtgtattt tatgtgaaaa atacactgaa cttactaaag aacatgtacc acctaaaaaa
     7141 tcagggaata ctggaggtaa aaagaccaga actggtaatt tagatgattt ccttaagagc
     7201 gattttacaa aaggtgattt tccgaagggg attaaaagga aacctcaagg aaatgtttat
     7261 tata
//
LOCUS       DACDKJ010000100         6745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDKJ010000100 DACDKJ010000000
VERSION     DACDKJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.103"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..115)
                     /locus_tag="IV328_002701"
     CDS             complement(<1..115)
                     /locus_tag="IV328_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8938768.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="IV328_002702"
     CDS             246..698
                     /locus_tag="IV328_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938769.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="IV328_002703"
     CDS             712..1401
                     /locus_tag="IV328_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938770.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..3877
                     /locus_tag="IV328_002704"
     CDS             1526..3877
                     /locus_tag="IV328_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8938771.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3957..4352
                     /locus_tag="IV328_002705"
     CDS             3957..4352
                     /locus_tag="IV328_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938772.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4677..5294
                     /locus_tag="IV328_002706"
     CDS             4677..5294
                     /locus_tag="IV328_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8938773.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5335..5634
                     /locus_tag="IV328_002707"
     CDS             5335..5634
                     /locus_tag="IV328_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938774.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5668..5835
                     /locus_tag="IV328_002708"
     CDS             5668..5835
                     /locus_tag="IV328_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8938775.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5880..6485)
                     /locus_tag="IV328_002709"
     CDS             complement(5880..6485)
                     /locus_tag="IV328_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8938776.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2319 a          949 c         1313 g         2164 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3841 tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta tttaaacagt
     3901 gtccttttcc tttttaaata tatacgataa aataagtata tgaattgtaa aaggacatga
     3961 tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat aattctggat
     4021 gtggaattat ctttatgttt gttatagttt tgggaatagt agtactttta ataactgtca
     4081 agatagtaga ttactttgcg aatactcctg aagaacaaat acagagaaaa cttcctgagt
     4141 ttgaaagagt accaccttat gatccaagag ataattcatt tcaagtaaaa aaattaaaag
     4201 agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa
     4261 agaaagaata caattatatt gtaaaggcaa aagtatcaaa aagatttatt tttaacccta
     4321 aattagaaat atatagtgtt gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa
     4381 gaagtcaaat tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg
     4441 ataactagtt tatttaatgg cagaccgtta aggcctgcca tttttatgta ttttagcgga
     4501 ataaatataa tcatgcggga gtgtaaactt actgtttcaa aaagtatact ttttgacatg
     4561 ccttgatttt ctttgtttat gtatggcata ataaccacat aatacaattt gaaacgtttc
     4621 tgatttaatc tgattgtttt gaaacaattt tagtgaaaaa agggtagata aaaacgatga
     4681 gaaaattttt ttatatccgg gtcagtagtt atgatcaatc gatcgaacga caactgattt
     4741 ccgcgaagga attaggaatc cctgaagaat atatatttgt agaaaaagcc agcggcaaag
     4801 attttaaacg ccctgaatat caattaatga aacggatgtt tcgaaaagga gatttactct
     4861 atctacaatc attagataga ctaggcagaa ataaacaaat gattttagaa gaatggcgag
     4921 aattaactaa ggaaaagcag attgatattt ctgtgttaga catgcctctg ttagatactg
     4981 ctagttacaa gcaactagat gggttagaga cgcttatctc tgacttaatt ctgcaattac
     5041 tcagctatat ggccgaagac gagcggaaac gaattaatag ccggcaaaga gaaggcgtag
     5101 aagctgcgaa acaaaagggt gtaaaattcg gcagaaagaa aattgcttta cctcctgaat
     5161 ttcctgcgat ctattctgac tggaaagcag gaaagataac tgcagtctat gctatggatc
     5221 aattgggcat gaaacgaaat accttttatc gacgggtgaa agaatatgaa cataccttgc
     5281 accacttttc ttgatagata taacagctat tagacgggaa gggagagttt gaaaatggac
     5341 ttaacgaatc aaagggtcgt ttttactgga acgctgcagc aaatgacacg aacccaagcc
     5401 atcggtttgg ttcactcttt aaacggccat tgtcaatcaa gtgttacttc aaaaacaaat
     5461 ttcctggttt gtggtcagta ttctaagtcg ttgtttgatg attctctcta tacaaaaaag
     5521 gaacaaaccg cgcgggactt gattgcgtta ggtcatgaga ttaccatttt atcagaagtc
     5581 gaattttatg cgttaattag tcaacagttg aaagaatggc aacggtattt ataatcgtta
     5641 gtaaaattta caaggaggaa aatttttatg aagcgaaaat cttatgatcg ttggggcatt
     5701 gaaataacta gttttaatcg aaatattgct tttcacgtgg agtgtgactg tggggaatta
     5761 gcaaaaagtg tttatcaaaa aaattacttt tactgtccta aatgccatcg ccgatacggg
     5821 gtacatggaa catagaaacg aaatagtgcg gtagaaaagc gaatgaatgt agtatatttc
     5881 tacaaatcat tcgctttctt tattttataa atagttggtc gagagatccc gttttctttt
     5941 gcaatctctg caactggaat gccttgcttt aacatgtcca ccacggtttt aaaaactaag
     6001 cgtttttgcg gatctgttga gttaatagaa tactcgattg gtcgtccctt atatcggccg
     6061 gctttttgag ccaataatac tccttgccgc tgacgttcac gaattttctc acgctcgttt
     6121 tgtgcaatat aacttagcag tgataaaaac atatcgaaca tagcggtatc caggagttca
     6181 tttccagtat taaaatctaa aaactgtgca tctaatattt ttaacgctac ttttttctgt
     6241 ttcataaaag caactgtatt ttttatatct tcataatctc ttcctaaacg atctagtgat
     6301 gtaacgatga cgcaatcacc ttcacggata taatttagta atttttgaaa ctcaggacgc
     6361 tcagtatctt taccactggc tttttcttgg aataatttat caatgttaag tccttccatc
     6421 attgccaatt gtcgatccaa attttgatct tgtgaagata ctcgaatata gccaattttt
     6481 cccataaaaa actccttttt aaaagtgata taatttacat gtaaaattta cttgtagttt
     6541 tactgctatc atagcatttt taaaaataaa ttttatgtag acttaaaatt tacttcatgg
     6601 agggtttaaa tggatgaaat caaaagtaac aaaaaagcat ttggcattta tttaggagaa
     6661 cctagtacag aacaattaaa ccaatttttt ggttctgttg cgaagtttga aaatcaaacg
     6721 cgccctctct gaactccaaa tatct
//
LOCUS       DACDKJ010000101         6448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDKJ010000101 DACDKJ010000000
VERSION     DACDKJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.105"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IV328_002710"
     CDS             complement(207..1169)
                     /locus_tag="IV328_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8938777.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IV328_002711"
     CDS             complement(1171..2610)
                     /locus_tag="IV328_002711"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8938778.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IV328_002712"
     CDS             2795..4741
                     /locus_tag="IV328_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8938779.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="IV328_002713"
     CDS             5005..5877
                     /locus_tag="IV328_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8938780.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6173..>6448
                     /locus_tag="IV328_002714"
     CDS             6173..>6448
                     /locus_tag="IV328_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8938781.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDV"
BASE COUNT         2069 a         1119 c         1142 g         2118 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aatcgtaata cccgctaaag catacaagga gagtgaacat aaatgactta
     6181 tacccatctt acaccaaacg agcttgtaat gatagaagca tattttcatc aagaaactcc
     6241 ggttgctatc gttgcgaagc agcttaaacg tggacgccaa acaatttaca atgtctataa
     6301 ctttctcaaa tgtggtggaa cagcacttga atgctttgaa caatacaaag aaaataagcg
     6361 acgttgtggg agaaccgaaa tcatttttcc tgctgaggaa aaagaataca ttgcaaaaag
     6421 atcaactgaa ggttggaccc cagacgtc
//
LOCUS       DACDKJ010000102         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDKJ010000102 DACDKJ010000000
VERSION     DACDKJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.106"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IV328_002715"
     CDS             334..714
                     /locus_tag="IV328_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8938782.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IV328_002716"
     CDS             696..1610
                     /locus_tag="IV328_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8938783.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IV328_002717"
     CDS             1855..2544
                     /locus_tag="IV328_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938784.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IV328_002718"
     CDS             complement(2665..2751)
                     /locus_tag="IV328_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8938785.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IV328_002719"
     CDS             3427..4377
                     /locus_tag="IV328_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8938786.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IV328_002720"
     CDS             4398..4931
                     /locus_tag="IV328_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8938787.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IV328_002721"
     CDS             5567..6100
                     /locus_tag="IV328_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938788.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACDKJ010000103         6184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDKJ010000103 DACDKJ010000000
VERSION     DACDKJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.107"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(627..1160)
                     /locus_tag="IV328_002722"
     CDS             complement(627..1160)
                     /locus_tag="IV328_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8938789.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1181..2125)
                     /locus_tag="IV328_002723"
     CDS             complement(1181..2125)
                     /locus_tag="IV328_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8938790.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            2762..2848
                     /locus_tag="IV328_002724"
     CDS             2762..2848
                     /locus_tag="IV328_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8938791.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(2969..3658)
                     /locus_tag="IV328_002725"
     CDS             complement(2969..3658)
                     /locus_tag="IV328_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938792.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3665..3943)
                     /locus_tag="IV328_002726"
     CDS             complement(3665..3943)
                     /locus_tag="IV328_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938793.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(3903..4817)
                     /locus_tag="IV328_002727"
     CDS             complement(3903..4817)
                     /locus_tag="IV328_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8938794.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(4799..5179)
                     /locus_tag="IV328_002728"
     CDS             complement(4799..5179)
                     /locus_tag="IV328_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8938795.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(5629..5916)
                     /locus_tag="IV328_002729"
     CDS             complement(5629..5916)
                     /locus_tag="IV328_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8938796.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(5936..6160)
                     /locus_tag="IV328_002730"
     CDS             complement(5936..6160)
                     /locus_tag="IV328_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8938797.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
BASE COUNT         1633 a         1152 c         1043 g         2356 t
ORIGIN      
        1 tttcgttttt ttaaaattgc ttgttatatt aaataatctt agtaatttta ttatataaca
       61 ttttattata taacaatgat taacgtcata aaaagtagtt ttgaattcat aatcgtttaa
      121 ataaaattat aagatcagtt atttaaacag ctagtagaca taatgtgtat actaccaagt
      181 aacgcctgta ccaagggatc caggcgctga ttttgtgaat ttttattggt ctaggttaaa
      241 agctcttttt aaggcttggt tttctgcggt tgtttctttc taattctttg tgaatattga
      301 tcggtcattt tttaaatact tgttattttt gtttgtgttt gttatgcttt ttacatatct
      361 catattcatt tattattctt ttctaaggtt aataatagga taatctcttt cagaaagact
      421 gctcgtacga gcagtctttt cttatttatc aagtttttct acaaatactt cctcattttg
      481 actgttgtag taattaggat tgagttttag ttcccctttt ttatttcctt tgttggccac
      541 tgataataac attgggctaa agcttaatat tcctagtagg atagaaccga atagcaagtg
      601 tttttttgtc ataagtattt ccttcttcat ttccatagct gccaccattt tttccaattc
      661 tttgtttgtt cgtccattgt ttctgttgct tcagaaagag tcgttgaact attctctgaa
      721 gactcttccg tataagtcaa ctgcagttgc tcctgaagtt tttcgatttg ttggttggct
      781 tgcagggtga gttgttgttg ctggtcgagt agtttttgta tatgagaatt attttgctct
      841 ttgagggcaa gttgttctcg taaaaattca ttttcttcac gcaactgatc tagcaacttg
      901 tttgcaacca cgtttttgtt tggtatataa gcatttttat cgttgttttg ttcatgttta
      961 tgcaacttat ctagcaacct ttgattgtct aagttgcgag caagttgcaa tactccagaa
     1021 cgcaatacaa ccttataacc acgctcattt ttagctatca tggaagaatc taatttttgg
     1081 atatgtttcg atattgcttg cctactcatc ttaaaaagat ctgacaattc ctttattgta
     1141 aaggtttctt gtggttgcat actggttgcc tcctaggttg ctactggaat aaattatcta
     1201 aatcagataa taaacttttt ttctcttttt ccgaaagtgc ggactctgtt tgtttttcaa
     1261 gagctttttt ctctttttct agttgtttga tttgttggag gagttcaagt tctcgtttct
     1321 tctgtttgat tttttcgtta attgttgtag cgggttcttc gtattctttt tgtttatcag
     1381 ctaaagccaa tgctttattt tggtcttctt tggttggtgc atgtttgtta aagtcgtctg
     1441 cttttttgat ctcaggtttg aacgtaaatt gatagccaat aacaggtttt ccccgtcctt
     1501 ttccgtattt ctttttgatt gttagtcctt tgaagattgc agttagttct tgacgtatag
     1561 gttttataac tcgtttatct atatcaggtt gcttataact tttaggaata tctaataatt
     1621 caagaaagtc ttctttggag aaatatgcga aacctttggt tctgtattgc tttaacagac
     1681 gaaacatagt ctttgcatag ccactttgta gctcgttgaa ttgttgtaaa gaatagcgta
     1741 cccactcgtc taaattattt aacaaaggca gtgctttttc gtaaattcgt atatctacgt
     1801 aaggattttc tacgtccccg ttgattttaa attctgtaaa cattacaaac atatcgtaag
     1861 aaagcccaga ctcacttcta cgaccaaagc gtaaagataa gattttttca taagttgctt
     1921 ttaaatcttc tacaaatcgt acatttgcag ttgctttgta gttacttaaa tcttttagtt
     1981 ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg cattctagac acgatggaaa
     2041 aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg aattgtattt aattcatggt
     2101 ggtattttac aatctcatta gccatataat cacctcaatc caattatgaa tgacattttt
     2161 taaatgtcaa gacattcata tttgacgttc aaacagacaa aatgtccttc ataaacagac
     2221 aaaatgtcct tcataaacag acaaaatgtc cttcataaac agacaaaatg tccttcataa
     2281 cattctgtat ccattggtag agtaaggaca cagcttacgg taaagaggtt taaaaagagg
     2341 tttaaaaaga gatataaaaa gaggggggct aattttcccg cctaaaaacc acttcttttt
     2401 tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc aaaaccttgc ttcctacggt
     2461 cgccaaaaag gtgtgatcgc tacgctctca cattgtactg cgcagtcgcc taaaggctcc
     2521 tttgctaaaa acatgttggt aaagaggcct ttgagcggtt ctgaatgctt atctaagccg
     2581 ttttaagaca gtcttggata aaaatcctta tctagcttta aagcgcctta caggcggtca
     2641 gggcaagccc taacaaccct aaaataagcg atacttaaaa acaccacaca agtttaattt
     2701 tttaagatat aataacttgt cgcctatccg acagtcttgc gactggaagg aggtgtatct
     2761 aatgtttgaa ttgttcattt gcccccttct tatagaagtt tgtgtctctt tattcagtta
     2821 ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg cgacatcaac ctgaaaaaaa
     2881 gccccagaga atccgacctc tctggggctt tcgtgtatct aatgtttgat catttgcatg
     2941 tttagtttaa catgattgcc gatcaaattc aattttgctg ttgctgatct aaccattcct
     3001 tgacactttt tagataattt aatgccgttt cttttttcgt tggattttgt atgttcgttg
     3061 cttgctgaag cagtgcttta taacgttcgt aggtaactga ttgttgccgt tcttttcgtt
     3121 cctgttcact agttgaaaaa gggattgtat cactgaataa attataacaa gcttcttctg
     3181 tctgcgtatc tttaaaatat tgcacgtctt ttttggttcg ctcatagttg tcttgaacct
     3241 ctttttgctg tttttctggc aagttttgat aaagacgagt gagtttttcc atgtttttat
     3301 ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat ttcctcataa caagtggtaa
     3361 actcttccat tttttgtgtt aacacttgct cctttttctt tttatcacat gctgcttcat
     3421 acttttgagt gatatcccct aataaaatac ttcttgaaat acgtttattt ccctctaaaa
     3481 cgccgaataa accacttaaa acgattgata atgtaaatat aacccctggc aataaaaacc
     3541 tcttaaatcg aattttagag ccttttaatg aatatcttgt ttgataagcc ttatagctaa
     3601 gccaaataat tcctaaaata ctggcaaaaa tgagtagata gggtaaatag aatgccattt
     3661 ctatttattc tccccatacc agccaaaagg ctaacagtgt catgattaag caacataagg
     3721 gggcaagaat atccattgat cgctgcataa ttactttttt ctgttcttta tttcgctggt
     3781 ttaatagcgc atggtggaat tttttgactt gtgaatcaat tgtagtttgt attctttctt
     3841 tggtataagg tgcaagaaaa gcacgttgtt tctttatttc attttctatg tcttgtaacg
     3901 ttttagaacg ataattcttg ttggtagtct gctcctcttt caacgtgttg gtcatggttt
     3961 cttcgagtcg gtctaatttc cggttgttca gtcgtaagag ttcgttgttc tcttcgtact
     4021 tcttttgcag tttcgcgaat gctagctcga gctcttcgta gctcgttggt tcgttgttga
     4081 ttgtaggctc gttctcgtct gtccagctca tgtaaaatcg tctccttttc gtaatccttt
     4141 cctagtttgt tggatcgtac ttttttcttt ttgtggatca attcgtaagt aaaatttttc
     4201 ccacgattca agcaatgaat gccgaatgtt ttaagagaat cacaaaattg gttatacgtt
     4261 cgagtttttt tgtttttcat tgtttgatca atcgcaaacc gaatttcgtc tttccaaaca
     4321 ggttcatttt tttcttttaa tttgatttca gacatggttc gtttttcggg tttcttccct
     4381 cgttcagtga ttgttaagtc atgttcaaga caaatctcgt catttaggtc ataacatttg
     4441 tttaaaaatg cttgaccatg ggcttgaaat ttgttcccag tttccagatt gacggagtta
     4501 atgacaatgt ggttatgaat atggtctttg tctgtatgtg tgtaaatcgc gatttggtgg
     4561 tcaggggcta tttttttcgc atactctgcc cctagttgat tggctttttc agcggtaatt
     4621 tctcctggtc gaaaagactg gatcaataag tgtgcttgta cacgatcgtc ttttccatac
     4681 agcatgcgaa tttgtttcat ttcgcttttg gcataatcaa tgtcacagtt tatccctgtt
     4741 ttgacggttg ctcgtggttc ggcgtaattg atcgctcggc tacaagaagt ggttcggctt
     4801 actttaactg ttgccatatc ttttgtagct ccttttgcat gatttttagg ttttcggata
     4861 agcgttgggc gtcgccagga tcaacgtcgt gttggtgtgt gttgcaccac ttggcaattt
     4921 gattgatatt cccgccagct ttggctaaat cgcgcgcgat tgcctgagca atgtctggcg
     4981 ccactttcgg tgtcaccaga cgcgcgtttt gtgccttggc tttcacgaaa gccggcacac
     5041 tcatttgcaa agcttcagct gactgcttca gctttgcaaa ttcggattcg ctcactcgaa
     5101 aactgatttg ttttggctcg gatcgatagg tatatttttt agaatgattg ctagccaaat
     5161 attgattttg ttcgctcata agtcccaccc tttcagtacg tcctacggac gtatcgttcg
     5221 caataccatt gtacctaaaa aggtacaata agtaaagctc actaaagctc actaaagggc
     5281 ttggcaagct ctatatttgc tagccaaata cctacgtatt tggtgcaaat aaccgcttgt
     5341 caaaggggcg caagctcccc cttgaatccc cctccccccc actctccaca atacaaaaaa
     5401 gactagcaaa tgctagccac tttttgtaaa gtttcgcttg tttcaccttc ggtgaaattg
     5461 ttgcaaatca ataattaggt agagctacat ttttttcgtt gttcaaagat tcttatgcca
     5521 tttactatta ataaatctat aaatatgata atttttcggc tctataattt atgggactga
     5581 tgaatcagaa ttgattcttc agtcccattt tcattttatt ccaaagaatt aactccaaat
     5641 accaatagaa gcccatcctc tatatacttg agattcctta ctaacaattt cttgtaattc
     5701 tattaaagat ttaggtaatc caaatttatg ttttaaaata tatcttccaa gaaaactact
     5761 taatttcata ctaacttcac caacactttt tccctcactg atttctttag aataaagaaa
     5821 gagcttttct tttaagtcag gatagtcatc tatagttgct tcattgtata ctttactaat
     5881 tgcatttaaa agtttttctt ctttagtaaa atccattata ataacctccc taccactacc
     5941 ttctaggtgc ccatggacca tgattaaccc aaccattaac aataattttt ccaatttctc
     6001 ctttagcttg attccaatct acccaacatt tttctttgtt acaataaaga ccatttccat
     6061 agtaagtagc tgcatcaact tttattcctg taccgatgcc agctagacaa gttcctaata
     6121 ttcctagaat aacacaatgt tttaatactt tctttttcat gattgttttt ccttttcgtt
     6181 tttt
//
LOCUS       DACDKJ010000104         6001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDKJ010000104 DACDKJ010000000
VERSION     DACDKJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.108"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IV328_002731"
     CDS             <1..395
                     /locus_tag="IV328_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938798.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IV328_002732"
     CDS             398..1228
                     /locus_tag="IV328_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8938799.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IV328_002733"
     CDS             1244..2095
                     /locus_tag="IV328_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8938800.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IV328_002734"
     CDS             2092..2451
                     /locus_tag="IV328_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8938801.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IV328_002735"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IV328_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IV328_002736"
     CDS             2624..2929
                     /locus_tag="IV328_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938802.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IV328_002737"
     CDS             2929..3240
                     /locus_tag="IV328_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938803.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IV328_002738"
     CDS             3407..3823
                     /locus_tag="IV328_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8938804.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IV328_002739"
     CDS             3823..4491
                     /locus_tag="IV328_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8938805.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IV328_002740"
     CDS             4488..4865
                     /locus_tag="IV328_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938806.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IV328_002741"
     CDS             4862..5161
                     /locus_tag="IV328_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938807.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IV328_002742"
     CDS             5403..5819
                     /locus_tag="IV328_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8938808.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DACDKJ010000105         6001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDKJ010000105 DACDKJ010000000
VERSION     DACDKJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.109"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            31..1005
                     /locus_tag="IV328_002743"
     CDS             31..1005
                     /locus_tag="IV328_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8938809.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            987..1448
                     /locus_tag="IV328_002744"
     CDS             987..1448
                     /locus_tag="IV328_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8938810.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1498..1962
                     /locus_tag="IV328_002745"
     CDS             1498..1962
                     /locus_tag="IV328_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8938811.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1976..2725
                     /locus_tag="IV328_002746"
     CDS             1976..2725
                     /locus_tag="IV328_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8938812.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2725..3555
                     /locus_tag="IV328_002747"
     CDS             2725..3555
                     /locus_tag="IV328_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8938813.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3555..4202
                     /locus_tag="IV328_002748"
     CDS             3555..4202
                     /locus_tag="IV328_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8938814.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4300..5076)
                     /locus_tag="IV328_002749"
     CDS             complement(4300..5076)
                     /locus_tag="IV328_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8938815.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5220..>6001
                     /locus_tag="IV328_002750"
     CDS             5220..>6001
                     /locus_tag="IV328_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8938816.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNG"
BASE COUNT         2038 a          875 c         1096 g         1992 t
ORIGIN      
        1 ataatactta ttagagtgag ggataaagaa atgagacaac tacacaaaga acagattgaa
       61 acagcaatgg cagcgttaaa ggaaaagaca attgatcatg tatattttac agcatgtggg
      121 ggttcacaag ccgttttaat ggctggtcaa tatctatttg ataaagaatt aactgtacct
      181 tgtcatgttt atactgctaa cgaatttaac tatgctatac caaaaggatt atctgaaaat
      241 tctttagtta tcacttgttc ccattctggc acaacaccag aaactgtaga agcaacaaaa
      301 aaagcaagag aatttggagc aatgactatt tgtttatcta atgaagaagg atctcctcta
      361 tgggaagcag ctgaatatcc tgttcactat gattggggta aagatgttga tgctagtgat
      421 ttaaataaag gaattcttta tggtttatta tttaatattt tacttgtttt agatggctct
      481 aaaaaatggg aaacttgctt gaatgaattt gataagttaa atgatcttgt tattgctgaa
      541 aagagcaaat ttgaaataac tgctaaagaa tggggtaaaa accaaaaacg agataaaatt
      601 atttacacaa ttggtagcgg tatcaattac ggagaaattt actccacagc agcttgctgg
      661 ttcatggaaa tgcaatggat taattctaat gctattcatt ctggggaatt tttccatggt
      721 ccttttgaaa tcactgatta tgatgtacca ttcatcttag tgaaaagtat tggagataca
      781 agatttttag atcaacgggt agaagacttc actaaaaaat tcagtaataa attaatggtt
      841 ttagaccaag ctgattttga cttagaatct atagatagtg tagcgaaaga atatattgca
      901 gctgtactta gtggcgttgt tattcgccaa atggtagaag cgattgcttt tgaaagaggc
      961 cattccttag atgttagacg ctatatgtgg caaatgaaat attaattaat aggaggttgc
     1021 aaaagatggc aaatcaatta tttcacattg tgattacaac acattctacc ctttgtgagg
     1081 gttatttaaa ggcaacaaaa ttaattttaa tgaatgacgc cacaggtatt agtgctgttc
     1141 catttaaaga aaatactagt ccagatgatt ttgaggagag tatgaaagaa ataattagtc
     1201 agcatcattt gcaacctcta attattttaa cggatttaat tggaggaaca cctaataatg
     1261 tttctattaa acagttgaac aacaatagaa ttcaaattat tgctgggatt aatttacctc
     1321 ttttactgga gttattaatg gcccagtcag ctggtaaatc tatagaagaa atagatttta
     1381 aggaattact gaataactct caaaaaagta tggttcatat taattcgctt ttaaaagatg
     1441 tcagttaagg tggcaatttc tattctataa attttaaaaa ttgaaggagg aaatagaatg
     1501 attaaattag tgcgtgtaga ccacagatta cttcatggac aagtaatatt ttcttggact
     1561 aaacagtatg atattaacca tattattgtg gctgataaca aagttccagg agatccaatg
     1621 tctgtgatgg ctttgacaat tgctaaacct gctgattgca aattagacat tgttaaaatt
     1681 agtgatgtta aagggattat ggaaacatcc ccaaatgata attacatgat tttaattaaa
     1741 ggacctaagg aagcttttga attagttcaa aatttaccag aaattaaaga aattaatttt
     1801 ggcggtgtgt ctaagaaaag cgagtccaaa caatatggaa aggcagtctt tttaactcct
     1861 gaagaattag aagccacaaa aaaaattctt gatcaagaag tgaaaatatt tgtgcaacaa
     1921 gtcccatctt caagtattga aaatgtagat tttagtaagt aaggggggaa aataaatgtt
     1981 attaaaagcc attttattag gtttagttgg tgtggtagct gttattgact ccaggttaat
     2041 tggtcgacaa aatattggta gaccactaat tttgagtaca cttgccggac ttgtgctagg
     2101 tgacttgcat acagggattg ttcttggggc atctttagaa ttaatttcta tgggattcgt
     2161 ctcaattggg gcggctggac ctcctaatat gcaacttggt agtattattg ccactgcttt
     2221 tgcaatttta actaatagta gtacagaaac agccttaaca atagctatcc cagtggctgt
     2281 agctggtgaa tttatgagta ttatcatgcg catgataatt gctcaatttg gacatgctgc
     2341 agataaagcc attgaaaata ataaatttag aaaagctcaa attattcata tttattggtc
     2401 atttattttt aatgcttttg tttactttat tccaatcttt ttaacagtat attttggtgc
     2461 tgacgttgtg gcagatttag tagataaaat tccagaaatg attacagacg ccttgactgt
     2521 ctctggaaat atgctttccg cattagggtt tgcaatgcta ttaagcacaa tgttgagtaa
     2581 aaaattatat ccttacttta tttttggatt ctttattgtt gcctattcag gtctttcact
     2641 aattggcgtg acaatttttg cagctctgat tgcctttgta atggatcaag ttaaatatgg
     2701 aaaaagagaa gaggcgcatg gttaatggaa aatagtacag aaaaaaaacg tttaatcaca
     2761 aaaaaagatc ttaataagat gtgtcttcgt tcatggacaa tcatgtgttc gtggaattat
     2821 gaaagacaaa tgcatatggg atttatgtat gggatggcgc caattttaaa taaagtttat
     2881 aaaaatgatg aagagaaaaa aagagaagct tatactcgtc atatggcatt ttttaactgt
     2941 acgcctcaaa tgacttcatt tattatggga ctttgtgctt ctatggaaga acaaaatgct
     3001 caagcagaag aaggaaactt taatactgaa tctatctcca tgattaaaac cagtttaatg
     3061 ggtccttttg ctggaattgg tgattcattt ttccaaggta ctttaagaat tattacattt
     3121 gggattggat tgtcttttgc taagcaaggt agtattttag gaccgatttt agctgttggc
     3181 ctttttgcta taccttctat tttagctgtt ttttacggcg gtaaattagg atatgagtct
     3241 ggcaataaat accttactaa attgtatcaa gaaggtctta tggatcgagt aatgcaattt
     3301 gcttctatca ttggtttggc tgtagtaggt ggaatggtgg ctagcatggt gccaattgtc
     3361 acccctttaa aattcactac agggggaaca gaacttgttt tacaagactt acttgattcc
     3421 attattcctg gattattacc acttgtatgt actttgggaa tatataaatt gattaagaaa
     3481 aatgttagta caaatatgct cttattaggt tgtattttgg cagggtttgt tttaagtata
     3541 ataggtatct tataatgatt aaggctctaa tttttgatat ggacggagtt ttaattgata
     3601 gcgagattat gtatcaaaag tgggtattta attttttaaa acaagaaaat ttaagctatc
     3661 ctttgtattc ttatcaaaaa aagattggaa ctacagcaag tatttttgat gatatagaaa
     3721 agtacaatga gggatgtaac agtttagaat tgaagggaaa atttactaaa tattggaaat
     3781 cgcaaaagat tgattacttg gatatttttc ctaatatact aattgaacag cttgaatggt
     3841 taaaaaagaa aaaaataaaa atggcaattg cttcttccag tccaatggat actataaatg
     3901 aagttgttac cacttgtaaa atagagtcat attttgattg tttaattagc ggaagagatt
     3961 tgccagaaag taaacctaat cctactatat tccttaaagc ttcagaacag ctcagaattc
     4021 cagttgaaga atgtctggtg atagaagatt cttataatgg tataaaagct ggtaaaagag
     4081 caaatatgaa ggttcttgca attaaagata aacgtttttc tcaagatgta agtctagcag
     4141 atgatgttat ttacgatatt aaatatctgc gacaagaagt acaggtgaaa tttggaatat
     4201 aaataagttt tttgatgtat ttagagaata ttagtaaaaa taaaaatcat gcccaaatct
     4261 aatcaaaatt gattgctttg ggtatgattt cttttaatat caatctatta ctttaggatc
     4321 tgaatcattt ttatgcaccg tatctaaagt gatattttct gtgacttcaa taacataact
     4381 aattctatca caacgaactg tggattgaga ataatgaaat ggttgattat ttttatcttt
     4441 gccaaccttt tctaaaataa atagcggatc tcctactata cattgcaata agtcggaaag
     4501 ctcaggtgtt gcagataaac cgtttatttc cataatagag ctcgtagctt tgacatcata
     4561 gtcatcttct agaagtttat ataatgaagt atattggcta cctttttcaa ataaagttgg
     4621 aaatttttct tctgatgcga aaaaattatc aattcccatc ggggcaccgt cttcccacat
     4681 taaacgttta atattgtaaa ctctatcagt tacattgatt tgtaatttat tagctaaaaa
     4741 ctcatcagcc tttataattt tagaatgtaa aacagtatat ctaattttat gtcctcgggt
     4801 agaaagagaa tcgctaaatc catgaactcc agctaatttt aaactttctt ttttttgagt
     4861 tacaaaggtc cctttccccc ttaatcgttc aactgttcca ctttctgcta aaagtttaaa
     4921 agcttttcga atggtaatct tacttactcc ataaatttta cataattctt cttctgtagg
     4981 taattgctct cctggaagat attttttttc acgaatatcg ttacgtatag aagcttctac
     5041 ttgtaaatat aatggtctat tatcttgaaa attcatagta cactccctaa aaagttatac
     5101 ttcattatat cacaatttat taaaacgtcg attgtaaaat taagctagac aactaaaaag
     5161 atcatttgtg ctacactcaa aatagttccg accaaagaat taataaggag tgagtacaaa
     5221 tgacttacac ccatcttaca acagacgaac tagtaatcat agagtcttat ttcaatatcg
     5281 agaaacctgt cgctcttgtc gcacaaagtt taaatcgctc tagacaaacc atttataatg
     5341 tttattcgtt tctcaaacaa ggaaaaaaag tgctagatta ttaccgtcaa tacaaacaaa
     5401 ataaatctcg ttgtggacga cgttcaaccg ttttatctga tgaacaatca acatatgttc
     5461 aagaaaaggt agctcaagga tggtcacccg atgtgattat cggccgtggc gagtttccaa
     5521 ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa aggccttttt gacacacagt
     5581 cgttacctat gaaggggaaa cgtaaaccga ataatcatca agaaaagcgt ggaaaacagg
     5641 cctttcgcag aacgattcat gaacggaaca gtctctatcc acacctgact aaagaatttg
     5701 gtcatctcga agtcgacacc attgtgggag ttaaacacaa gagcgctgtt attacattgg
     5761 ttgagtgttt atccaaggtt attattactt tgaaaccgac aggcagacga gccgaagata
     5821 tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa acacttgttt aaatccatga
     5881 catttgattg tggaaaagaa ttttccaact ggcgatctat aagtaactcg aacgatattg
     5941 ccatcttttt cgcagatcca ggaacacctt cgcaaagagg gctaaatgag cactcaaacg
     6001 g
//
LOCUS       DACDKJ010000106         5930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDKJ010000106 DACDKJ010000000
VERSION     DACDKJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.111"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(170..3319)
                     /locus_tag="IV328_002751"
     CDS             complement(170..3319)
                     /locus_tag="IV328_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8938817.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="IV328_002752"
     CDS             complement(3333..4550)
                     /locus_tag="IV328_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8938818.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..>5930)
                     /locus_tag="IV328_002753"
     CDS             complement(4540..>5930)
                     /locus_tag="IV328_002753"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8938819.1"
                     /translation="LLSDEESKEDLIATIVDILGYSISPEYLFNVLADQAKQATFQLN
                     DLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDG
                     DVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGS
                     GSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDW
                     PTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLK
                     ETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKK
                     NRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKEN
                     DYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAW
                     LQGALEVFKHEQ"
BASE COUNT         1621 a         1259 c          992 g         2058 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt
//
LOCUS       DACDKJ010000107         5903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDKJ010000107 DACDKJ010000000
VERSION     DACDKJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.112"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..985)
                     /locus_tag="IV328_002754"
     CDS             complement(<1..985)
                     /locus_tag="IV328_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP8938820.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="IV328_002755"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="IV328_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8938821.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="IV328_002756"
     CDS             1810..2964
                     /locus_tag="IV328_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8938822.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="IV328_002757"
     CDS             3179..4147
                     /locus_tag="IV328_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8938823.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="IV328_002758"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="IV328_002758"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8938824.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..5785
                     /locus_tag="IV328_002759"
     CDS             5177..5785
                     /locus_tag="IV328_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8938825.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1637 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     5581 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     5641 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     5701 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     5761 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     5821 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     5881 gatagtattg aagatgtaag gca
//
LOCUS       DACDKJ010000108         5887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDKJ010000108 DACDKJ010000000
VERSION     DACDKJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.113"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..495
                     /locus_tag="IV328_002760"
     CDS             <1..495
                     /locus_tag="IV328_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8938826.1"
                     /translation="SEVLPAIRKHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRL
                     IAEQKIAEYEPKISYLDSILSSTDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQ
                     WLLCKKHMNQGYTKSHTTEIPKADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDL
                     EEIG"
     gene            510..713
                     /locus_tag="IV328_002761"
     CDS             510..713
                     /locus_tag="IV328_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8938827.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            729..1049
                     /locus_tag="IV328_002762"
     CDS             729..1049
                     /locus_tag="IV328_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8938828.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            complement(1231..2013)
                     /locus_tag="IV328_002763"
     CDS             complement(1231..2013)
                     /locus_tag="IV328_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938829.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITERFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            2064..2771
                     /locus_tag="IV328_002764"
     CDS             2064..2771
                     /locus_tag="IV328_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8938830.1"
                     /translation="MQELVILKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQK
                     YADLFWEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEK
                     AIRKNTAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVL
                     GGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRM
                     EIDQLNRQQRLFGDENE"
     gene            2949..3290
                     /locus_tag="IV328_002765"
     CDS             2949..3290
                     /locus_tag="IV328_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938831.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            3283..3954
                     /locus_tag="IV328_002766"
     CDS             3283..3954
                     /locus_tag="IV328_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8938832.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3960..4646
                     /locus_tag="IV328_002767"
     CDS             3960..4646
                     /locus_tag="IV328_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938833.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            4653..5504
                     /locus_tag="IV328_002768"
     CDS             4653..5504
                     /locus_tag="IV328_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8938834.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            5516..5785
                     /locus_tag="IV328_002769"
     CDS             5516..5785
                     /locus_tag="IV328_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938835.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
BASE COUNT         2215 a          915 c         1165 g         1592 t
ORIGIN      
        1 agtgaagtgt tgccagcaat tagaaaacat ggaggttatc taactccaga aaaagtagaa
       61 gaagctttgc ttaatccaga tacaatcatt caattagcaa ctcaactaaa agaagaaaga
      121 actggaagat taatcgcaga acaaaagatt gcagagtacg aacctaaaat ctcctattta
      181 gatagcatat tatcttctac agattcagta acaattagtc agattgcagc agattatggg
      241 atgtctccac aacagatgaa taaattactt cataaactag gtattcagaa aaaagtcggt
      301 aaccaatggt tattgtgcaa aaaacacatg aaccaaggat acacaaaatc tcatacaact
      361 gagatcccga aagccgatgg tggcactaaa attgtaatga ataccaaatg gacacagaaa
      421 gggcgtctat ttatctacga attactaaaa aaagaaggat attaccctca aatggattta
      481 gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc actcaacgag
      541 atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac accatgctcg
      601 ctgaaagatt cggcattagc aaaagctact tatctttaat tttaaacggt aaaaagactt
      661 ctgcagaagc aaatagaatc atagattcga ttattactat gtacgaattg taaagggagg
      721 aacagctaat gcaatatcta gaagcgaaaa tcccagttcc agaaggctat gtaattatct
      781 cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac tggtagatgg atgacgttgc
      841 cagaagtgct agaacggatt aacagaaaat atgattggtt tacttctaga gttttaaaga
      901 acccaagata tagaaatatt atcgatatcg aaaaaaacaa aaatggattt gtctattatc
      961 cagttgaagg aagagacaca tatctatttt taagaagtaa aacacttgaa tttttagaaa
     1021 caaatttttc ggaaatctta aggaggtaag cggatgggta aatttaatag agcactagta
     1081 ttcagcgcac cactaattat ctacgcttta ggtctttggg gaagcagaca agcattaatc
     1141 ggcacaattg tatatatggt ctggatcttt atggggcttg atgaagctga atatagagcg
     1201 aaaaagccag tcgggaggga ctgacttaac ctaattaatt aaaaattgta acaaataacg
     1261 caaaaataaa gctaatgaaa ttgtattgtt aggatctact ccaatagtta tatttacaat
     1321 ttcggttact actagtccta tcaaagctgc cccagtttgc tctaaatacc acattttatc
     1381 tttgtagaaa gcaggtgaag cttgttgttt ttcagatggt tcattagtta tatctttgtt
     1441 cttggctgta tttatttctt caaaaagctt tatagaatca tcaaggactt tcctttgctc
     1501 attaaggata tcaaaaaact tagcctcaaa ctcatcagat ggattagaag tgtctttaaa
     1561 aagtagattt tttgtactaa cttccggttc ggaagaaaat gctttttcta attcttgggt
     1621 agctacccct attctgaagt cgtacttaga catgtcaacg gcaaatcttt ctgtgatttt
     1681 agagatatca gaaaaataag gttttggcat agtagctgcc atttttttag caatattaaa
     1741 acttggagat tctaattgtt tctgtattgc gaacaaagaa ggaggaatca tattagcaat
     1801 agatttgtta cgtttctcga caatttgaat gagagaatca ttgagtattg cttgcgaagc
     1861 tataaaagta tcttgaattg caattaattt tttagcttgt tctatcgcag gctgcatagc
     1921 gttaacgaca tctagagaag gagcaatggt ttcaccgatt aattttgaag aagctatcaa
     1981 tttttgataa taaggagtat ctgaaatatc caattatata caccaccttt ttacctaaat
     2041 tataccaaaa gggagagaaa aaaatgcaag aattagtaat tttgaaaaat aaagaagctg
     2101 tgactacgag cttacaagtc gcagaaacat tccgaaagga acatagagca gttttaaagg
     2161 cgattgacga tctaaaagat gggcttgcac aaaagtatgc agacctattt tgggaagatg
     2221 tgtacatcca tccgcaaaac aaacaggctt atcgaattgt ctatatgaat cgtgatggct
     2281 ttactcttgc tgtattagga ttcaacaata ctcaaaaagt tttagagttc aaattgaaat
     2341 acattgaagc gttcaacgaa atggaaaagg caattcgcaa aaatacagct tttgacacaa
     2401 ttgaagacat gatgatctat caattagaag aaatgaaaga tgttaaaaaa gatgtttcca
     2461 tgcttaaaaa tactatgcga attagcggac aacaagagtt tgaaattaag caaaaaggaa
     2521 atacgaaagt tatggaagtt ctagggggaa aagaaagccg agcttatgaa gaaatcagca
     2581 aaaaagtatt ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg
     2641 gcgagttacc tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttaccag
     2701 aaactgcgat tcgcatggaa atcgatcaac tgaacagaca acaaagactt tttggtgatg
     2761 aaaatgaata gagctgaagc gctaagaatc gggacggtaa ttgctaatcg ctggtggaga
     2821 cacaataaac caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa
     2881 ataaaaaagt gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat
     2941 tttagcatat ggaaaaagaa ctttccactc tagatcaata tttgactgat cctagttggg
     3001 gcaaatcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacggatg
     3061 aagaactagc atgtgatcaa gacgatttgg gaaattttgt gagtatttgg gatcatgtct
     3121 atcttatcca tttatcgagg aagtccaaaa aacctgaata tatctatgtc atcgaagatg
     3181 gcttgattga tgcgctagaa gagtatgaca gagataactt gattgatatc tcttattacg
     3241 gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aaacaaaggg
     3301 acaacgactt ttgaaaaact ttttagtcgc aagttaaata aaatactcaa gaaaaaagga
     3361 aattttgatt atttatcttg ggctcacgca tgggagatta tgaaaaagaa tgatccacag
     3421 gcaacggtaa ctattaatga gtataaacac tacagagttg tttctggaac tcatcaagac
     3481 tttcttgttg aggaatataa accttttctt atggatgaaa ctgggactta tgtatctgtc
     3541 tcagtaacgg ttaaaggaca cacggaaacc gaattatttc ctgttttaga ttatcgaaac
     3601 caaccagttg ttaaaccaaa tgcaatgcaa atcaataact cattgaagcg atgctttgtg
     3661 aaagcattgg ctctacacgg actgggatta tatgtatttc aaggggaaga tattccaaca
     3721 ccacctagaa tcgatacgaa gaaattaaac atgctagaga cgattctaga agctttcaat
     3781 gagcagatgg gtaaagatat gaccaaaacc ttaatcgaat atgttaatga gcagacagat
     3841 aaattagggc tcttagctga taacgttgaa actattgaac agttaagcta tgagcaatgt
     3901 gccttgatgg agcgagcaat agcagctaag agaaaagaat tagataagaa gtgatatgag
     3961 tgtttaaacc attaatcgat tcatattcag cggttctgaa aaagttcaaa ggaaaagaca
     4021 taggtgcaac catcaatgag gaagtgaaca ttgatcgact aaagacgatg tatgacggct
     4081 acgatggtga tcgagttatt gaaattcgtt ttattgatcc tagacgtttc acagtacagc
     4141 aacgaaactt catctatgca ctcataggcg atattttcat cgatacaggc atgccaacgg
     4201 acttctggaa ggaattcttt tacttccgct ttgaaggtgt cacagggcgc aaaataagcc
     4261 tcaaagatga atcgaatacg actgtgagtg atgctaacgt cttagcaaat atcatcttag
     4321 atttcatctt tgaacatcat attcctttca aagaaggcta tgagatttta ccagcgaatc
     4381 aagaatatta cttctacaaa tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag
     4441 gagctgatat cgatcacttt gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc
     4501 attcagagta cacatttgca gcactctgca gaatccatca cacggagaaa cacaagatag
     4561 gtgtgatcaa tttcaaaaat aaatatcaaa tcaaagggat caagttaaac caggaaacaa
     4621 ttaagaaact tagaatagga ggataaaaaa tattggctga caacaaacga tactactatt
     4681 taaaactaaa agagaacttt ttcgatagcg acgagatggt tcttttagaa agtatgccag
     4741 atggatacat ttattctaat attcttctca aactttattt aagaagtctg aaacatgaag
     4801 gcaaactaat gtttaatgac aggattccat tcaattctac aatgcttgcg actattacaa
     4861 gacactctgt gggagttgta gaaaaagcag tacaaatatt tcgtgattta caacttattg
     4921 aggtattaga taacggagca atttatatgt ctgatataca aagttttatt gggaaatctt
     4981 caactgaagc tgataggaag agaaaataca gaaaagaaat tgaggaagca aaacagaatt
     5041 taataacaag tggacaaatg tcggacaaat gtccggacaa aactacacca gagttagaga
     5101 tagagttaga gaaagatata gagttagaga aagatataga gaatgtaacg ccttcgaaaa
     5161 aatcgaaggc taagcccgtc cgtcataaat acggagagta taaaaatgtt cttttgtcag
     5221 atgagcagat ggagaaactc aaaatagaat tccctaatga ctaccaagag cgaatagaac
     5281 ggctatctga gtattgtgaa tcatctggta agacttataa aaactatttg gcaactattc
     5341 gaagctgggc aagaaaagaa aaaagtgaat ctaagaacgc aagcagtgga tacaagcgca
     5401 caggaagacg agagaagctt cctgagtggg caatcgacca agaagcctat ctcaagaaaa
     5461 aagcgctaga acgagctaat agacaatcaa aagcaccatt ctaagaggtg ggaaattgaa
     5521 aatcgattat ctagaactaa ttaatgaaat agcaaagtat aaaactggtg aggaaataga
     5581 aatcctgaga gacgtatatg atcaactcga agaagctgga atcgaaggaa ttaagaatga
     5641 tcgttcgagt tggagtaagc tcagatacta tttcgcactt tatatcgata caacacaatt
     5701 aagaaattta gcatatacca aattactatt tgttgattgt gttaaaggct tgcaaaaaca
     5761 tcttaatgaa cttgagcagg tgtaatcaga tggacctaaa gacatttaca gcacagattg
     5821 aactaatgca tcaagaagct ttaagacaaa gtgtatcgta cgaagacaag tggctcaaca
     5881 cgttcca
//
LOCUS       DACDKJ010000109         5802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDKJ010000109 DACDKJ010000000
VERSION     DACDKJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.114"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..530
                     /locus_tag="IV328_002770"
     CDS             15..530
                     /locus_tag="IV328_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8938836.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            826..1107
                     /locus_tag="IV328_002771"
     CDS             826..1107
                     /locus_tag="IV328_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938837.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            1109..1525
                     /locus_tag="IV328_002772"
     CDS             1109..1525
                     /locus_tag="IV328_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938838.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
     gene            2182..3336
                     /locus_tag="IV328_002773"
     CDS             2182..3336
                     /locus_tag="IV328_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8938839.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            3330..3752
                     /locus_tag="IV328_002774"
     CDS             3330..3752
                     /locus_tag="IV328_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938840.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4875..5696
                     /locus_tag="IV328_002775"
                     /pseudo
     CDS             4875..5696
                     /locus_tag="IV328_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271903.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT         2223 a          961 c          930 g         1688 t
ORIGIN      
        1 tataaggagt atttatggca gataaaacga tcaaagacct tgctgaagaa ttaggagtta
       61 gtaagacagc tattaacaaa aaagtttctg acgaaaatcg aaaactttgg tttgcgaaaa
      121 tcggaaacaa gtttgtgatt aacgaaatcg gtcaaaaagc tataaaatca atgtttttta
      181 ccgaaaacga aaactcaaat caacaaccag ttggcgaaaa atcgaaaacc gaaaaccata
      241 aaaacgccaa ccaagtttcc gaattagaac tctttttttt cctagaaaaa caattaaatc
      301 aaaaagatat acagctacaa gaaaaagacg aacaattaaa acaaatgcaa aaattactcg
      361 accagcaaca acaactcacc ttacaagcca atcaacaaat caaaaaactt cacgaacaac
      421 tccagctaac ctatgcagaa gaatcttcag aaaaagaacc cctttctgtt actgaagaaa
      481 ctcaaaaagg acaaaacaag gagaaaaaat ggtggcaatt ctggcaataa cccaacaatt
      541 tttattcata aaaattgttg ggttactttc tttttttgct tttacaagca tagcgacatg
      601 atctcacgtc gctatttttt aaagagcata aggaaacgcg gtatcacttt cttacgaaag
      661 gtgctaggta ggctgtgcct accacgtttc taagaaaaaa atttgctatc actttttcgc
      721 cattgtggct agcttatgta tagtatttcg gtaaaaaaca gctatacaaa tactatattt
      781 gtatgatata cataaattat acacacataa gaaaggacgt tttctatgga cattttagtt
      841 cgaaatatca atccaaaaat tattacaaga gtagataaac gttgtcagga acttacaacc
      901 aaaacaggag aaaaatggag tcgaaataaa tatttaaaag tgctaatcga aagtgatttt
      961 gaccgtcaac ttatgaatta caaaaaagag caattcgatt tattactcga aaaatttatc
     1021 gatattcaaa caaaaaatac tgaaacattg aacgactatg ttcatacaaa caaccaactc
     1081 attgaaacac ttatcggagg aatataacat gagaaaaact tctgttcaaa aacacgttac
     1141 cctttatccc agtcacattg acgttttaga ttccatcatc gaaaaaaatc cagatatcaa
     1201 taactactcg gaagccattc gctttttatg tatgagttat gaacctgata ccaatacaac
     1261 aactaccagt aaccaagcca aactaaatgc gatgggaaaa gaaatttcta tcatgaccga
     1321 aattatcgca gaatttgctg atttgtattt aaaagataca ggcgttctta tcggtacaaa
     1381 ctcgaaagta tatcaagaag taaaagaaaa aattgaaaaa cagctaaatc cctcccaacg
     1441 aaatcaacca aagaatcgtc aaaaagaaac gagtcaaaca ttcacttcta aaaatttcga
     1501 cccaaccaac tttctaaaaa gataaaaaaa taaaccagga aatttttaat ttcctggttt
     1561 attttctaga gagcggtctt ccaataagat ttgcaaatcg atcccaattt gcgtaaaaaa
     1621 aaagctgctc gtacgagcag ctaaacaaga aaaagtcagc ttgttttaag ggtaaaataa
     1681 gtgtaacaaa ttttgcgaat gcaacgatct tttttattat acgcaaacaa tcaaaaaaaa
     1741 agactgctcg aaagagcagt ctcaagcaaa aaataaacga acaaacgcaa ttactcacga
     1801 acacaaacaa atacaataaa atacctaaat aatgaaaata agtgataaaa aacaagggta
     1861 tttatagttt aacaagcgct cataaaaata acaagtcttt aaaaaatgac agataaaaat
     1921 gcacaaagaa tcggacacag ataaccgcga aaaatcaagc tttagaaagt tgtttttagc
     1981 tagaccaatg aaaaatcaca aagtccgccc ctgtatccgt tggtagaggg gctacttggt
     2041 ttagtgtaca ttatgttaac tagctcgtta aataacgatt atgttcccaa aacaatattt
     2101 tatgaactat aacaaaaatt tgttatcgca tattgtatat tttaattata ggtgtatatc
     2161 acttaatttt aacacaaagg cgtgattatt ttgaaaccca aaaataaaat ttatctaggt
     2221 ctcctctttt ccttgatgat tggaattttt agtataggaa ttacaaatac ctatgcttca
     2281 gaaaattcta atttcaatat caactcccaa ccaattgaag aacaagcaat taattttgcg
     2341 aaaactgatt ttgaacactc cataacttca attcttttaa tggaaaataa cactgaaact
     2401 gatattaact atactcttgg ttcgccattt aaaatgctcg atggagaaaa tacggcaaat
     2461 agttctattt tctttccagt attttcagac gctgataata cgattaaata tgtttatacc
     2521 gtcaaaaaaa atacaaatgg agaatattca ggaagtattt cacaattttt agcaaaagaa
     2581 cttcaaaatc tagtagatag caatataaca gatacaacct tattttttca agataatagc
     2641 atttattatt ccgataatgg ccaaattaaa ctactttgga aatcaccttt aagtacagaa
     2701 aaaaatggtg tctcttcctt tacagaaaaa aatgatctta tatccgtaaa tataaaaaat
     2761 aaaattaaaa atttctctaa aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc
     2821 tcctccggag ataactttat tatgattaat tggcgtatag cagaaaccca aacaagtttt
     2881 ccgtggtgcg ctgcttacgt agaagctgct atcttatgta ataaaacaga tgtacgcgct
     2941 acttcagcta gagccattat gcattatacc tatcctaatt taaacaacag tcaaattgaa
     3001 tcaaaaagtc ttaatcaaga tcaaataata aaatatgcaa attactgtgg tgtttatcct
     3061 aaaaaagtag cacgaatact aaacctttca gaggtccaaa cacaactaag aaaaggaaat
     3121 gccatttata tgggaactgc tggttatgga aattatagta acagccgtca tgcacttgca
     3181 ttatttgggt gggtcaatca aggtggttat caaacttact atgtgtggaa tccttggtat
     3241 aactacccaa ctgtaataag tgcaaatgca gggccagtta caattcctgt acctggaggt
     3301 ggttttactt gggacaatac cattacaaat tggtaaataa acccattgtt ttagttatgg
     3361 gtctagtgtt agttcttttc ttaactagtt gttcaaaaaa acaagaaata caaaagactg
     3421 agctgattcc ttttctagga acttggcaaa ctaaagatca ctcagaacgt ctagtaatca
     3481 ctaatttaaa tacttcaaaa aatatagaac aatataccat cttagtaaaa gagaacaatc
     3541 ataaaattca aatcattgat aaaaatttaa atataaatga acaagttttc atttacaaag
     3601 caacaaataa tttactaaca ctgcgctcta ataaaaaaca attagattac acttttagtt
     3661 tggattctcc aaaaaagctc gtatttattt tgacaacgaa tgatgggaca gaagggacag
     3721 ctaaaccaat tgaatttttt aaagctgatt gatgaatagt tgtttgatat tacaaaacaa
     3781 taatttcacc gaaggtgaaa caagcgaaac tttacaaaaa gtggctagca tttgctagcc
     3841 ttttttgtat tgcggagcgt gagttttgag ggggattcaa gggggagttt ctcccctttg
     3901 acaagcggtt atttgcacca aatacgaagg tatttggcta gcaaatatag agcttgccaa
     3961 accctttagt gtgttttgta gaaagggacc ttatagtcac tttcacatta caaacgatac
     4021 gtcctatgga cgtatcaaaa gggtgggact tatgagcaaa ttaaagcaaa atttggctag
     4081 caatccttct aaaaaataca cctattgatc agagtcaaaa taaatcagtt ttcgagtgag
     4141 cgaatccgaa tttgcaaatc tgaagctttg caaatgagtg tgccggcttc cgtaaaagtc
     4201 taggtaacat tagtcgatat atacataagg ctctaatttg cgttttaagg cgttttaaac
     4261 gttctggtat gtaatttata ccaaagcgac tttaaaacgc cttaaaacga attttatagg
     4321 tcttgtagga cgtttcaggg ttgttagggc ttgccctgac cgcctgtaag gcgctttaaa
     4381 aacaagataa gggattttat ctagacttgt cttaaaacgc cgtagatgag cgctcagagc
     4441 ttcttaaagg actccttacg aactgttttt aagcaaagga gcctttaggc gactgcgcag
     4501 ttcaatgtga gagcgtagcg atcacacctt taaagcgacc gtagggagca gattcccagt
     4561 ttaggaaatt tcgcgaacct gtttggagaa aaaaagtagg aaatttttag gcgggaaatt
     4621 ttgcccccct cttttataat ctcttttata acctcttttt aaacctcttt accgtcggct
     4681 gtatccttac tctcccaagg cctgtagctt ttttagggca tgaaattatc tgtttatagg
     4741 catgaaatta tctgtttata ggcatgaaat tatctgttta taggcatgaa attatctgtt
     4801 ttaggtattg ttttaagaca taatatacct taaaattatg tcaataaaat tagttagact
     4861 ggagtatcaa ataaatggca aatgaaatcg taaaatataa accagaatta aataccattc
     4921 ctttaaggaa atttactcca atagaaatga acttgttttt ttctattgtt tctagaatgc
     4981 gtgataaagg taatcaaaaa atacgctttt atttcgatca gcttaaagat ttaagtgctt
     5041 ataagcaaac tgctggagaa gtctttataa aagatttaac aagaacatac gatcatttaa
     5101 tggatttacg ttttggaaaa aaaagcaatg gtggattaac catagaacga tttacaatgt
     5161 ttactgattt tgaaattcat ggagaagccg ataaccctta tgtggatata aaagttcacg
     5221 aaaaagcttt gcctttactt aacaatttag aaagttgggt acgtttctct ttaacagaat
     5281 ttcgagattt aaatagcagt tatgctaaaa caatgtttag attactaaaa caataccgaa
     5341 cacaaggatg ggctgaattt tccaaggaag attttttaga actattagat attcctaaaa
     5401 gctatagaca aacaaatata aatcagtttg tactcaaacc cataaaagaa gaactcacac
     5461 cattatttaa aggattaacg attcgtaaaa aatatggtaa aggccgaggc aaacctgtta
     5521 ttggttatcg ttttacttgg aaagctgaaa ttaatcatgc tgacgatttt tcaaaaggga
     5581 agcaagaaga tttacgtaca aaattattta atattgaaca caatggagaa ttaacgcaag
     5641 aagaaaaatg gcgcgctaaa gatcgaatac tagatcttcc gttaggtact catgaagcta
     5701 attttaaaaa acaacaacaa actgaaaaag aagaagtgga aaaacaaaca ctttcaaacg
     5761 aacttaaaca agatttatta ggaaatttac aaaacttatt cg
//
LOCUS       DACDKJ010000110         5790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDKJ010000110 DACDKJ010000000
VERSION     DACDKJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.115"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV328_002776"
     CDS             complement(99..1229)
                     /locus_tag="IV328_002776"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8938841.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IV328_002777"
     CDS             complement(1877..3079)
                     /locus_tag="IV328_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8938842.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IV328_002778"
     CDS             complement(3139..3342)
                     /locus_tag="IV328_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8938843.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IV328_002779"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IV328_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IV328_002780"
     CDS             4879..5691
                     /locus_tag="IV328_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8938844.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DACDKJ010000111         5359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDKJ010000111 DACDKJ010000000
VERSION     DACDKJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.116"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IV328_002781"
     CDS             25..696
                     /locus_tag="IV328_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8938845.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IV328_002782"
     CDS             755..1195
                     /locus_tag="IV328_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938846.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IV328_002783"
     CDS             1207..1875
                     /locus_tag="IV328_002783"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8938847.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IV328_002784"
     CDS             1939..2562
                     /locus_tag="IV328_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8938848.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IV328_002785"
     CDS             2618..3004
                     /locus_tag="IV328_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8938849.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IV328_002786"
     CDS             3217..4608
                     /locus_tag="IV328_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8938850.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1778 a          883 c         1124 g         1574 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagtc taatggtgat
     5221 aagtcaagat gaaatttaag attttcttta ttgagtaata tgttttgaag cgcacgaaac
     5281 tagacccgat caaaatcaaa tttttttcgg gttgattcaa ggatttgcgt caataacgga
     5341 ttgttatttc tctatcaca
//
LOCUS       DACDKJ010000112         5249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDKJ010000112 DACDKJ010000000
VERSION     DACDKJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.117"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1665)
                     /locus_tag="IV328_002787"
     CDS             complement(<1..1665)
                     /locus_tag="IV328_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8938851.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQN"
     gene            complement(1707..2765)
                     /locus_tag="IV328_002788"
     CDS             complement(1707..2765)
                     /locus_tag="IV328_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8938852.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(2884..3339)
                     /locus_tag="IV328_002789"
     CDS             complement(2884..3339)
                     /locus_tag="IV328_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938853.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(3923..4726)
                     /locus_tag="IV328_002790"
     CDS             complement(3923..4726)
                     /locus_tag="IV328_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8938854.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1550 a         1026 c          727 g         1946 t
ORIGIN      
        1 attttgacca tatactcttt ctttcctagg tggaagacgt aaatctatat ttaaatgttt
       61 tcgtactaat tcagcactct tcaacatttc attataagca atttccagaa gagtttgttc
      121 ggataaagcc caaaatggaa ctattcgtcc aaatcggtta ataacttcag gctttagacc
      181 ttcaccaatc aaggcttgtt tgatccctga tgcgactttt cggtattctc tttttagttc
      241 agtgctgtat tcattagaat tttccttgtt tgtaacacga gatagtgagc tatatcggtt
      301 taacgaatcg aacatctccg cacctgcatt tgttgtcgca aataaaattg tatttgacaa
      361 tgaaacggtg tcgtgaactt ctcgatcatc ccatcctctt tcagtaaaac ttatcctacc
      421 ttcatctaaa attgataaaa aggcatcgag tgtttctaca ccagctttct catattcatc
      481 acacacaata atcgcagaag gatcatgcct tacatgagta cctataagtc gtctaaaacg
      541 cattgcatca tctttggttt tgtagtccgg catattaact aagagaactt tattttcatc
      601 gccataaagt cctcttgcca cggctttagc cattgcagtt ttaccgaccc ctgttgatcc
      661 tgtaaacaga gcagttgctt ttggttgctt tcgaccttta aaacgatcaa atccaagctt
      721 catacgttta aacaacattg ttatagtata aactgctaac ggttgtcctt ttatggactt
      781 taattcctct tgaagttttt ccatatcaaa atcagaatct agctgaatat tatacttacc
      841 aaatatttgc aatagacttt ctcgattata agcatttccg tctactcgat cttttgcaat
      901 caaatcctcc attacagcaa ttgatttagc cggttcggac aaatcctcac gataaagttt
      961 gtttgcttgt acgatttctt gaagtaattc atcaggaatc ctttgcgata atctaaaaga
     1021 agcctgagga tcatctttaa cagaggatgt atttagccat tgtctcaaaa tatcgacgac
     1081 aacatcgctt tttagttcat tcaattctaa cggttgtagc cgtctcccta aggcatcatc
     1141 tcttttgata tagttttcat attcttttct tgtagtagcg gtaatcattt gaagtgattc
     1201 atttgctaac ggttctttta gcgaatcacc cccaatttta ttgccactcc caaaagagct
     1261 aacaacttta tgaaactcgt caatgaagag gacaaattca ttatctttgt cctcagcttt
     1321 taaaattcgc tcatattctt tcaatcttgg aattaatgtt tctaaacgtg tttggaggac
     1381 atcatctccc ccaacacgca attgtccgat tgataattta tatacttgta ctttctttcc
     1441 atcttgtcgc tgtctttcag cccattcact tgcaagtgct gttttacctg ttccggcttc
     1501 acctaacaag atagcttgcg gtgtttccgg tctattcaag atcagtgaca actcatggag
     1561 ttcgtcatca cgtcccacta acttatgttt tggtggtttc attggtgtga gagcatgatt
     1621 aaattcggtt tgaatattct taaaaaactc ggtactattt tccatctgtt tgtgttcact
     1681 tcctctaaaa taagaataat ttacttctag tcagtcaaag ttaggtactt aaatgtcgtc
     1741 ttcatgtctc caaaagaatc tttgttaaag tcagcatttt tgtattgatc aatcaatttt
     1801 tgtgtatcat tgattgcttt aatttcggtc tccattcgac tgtctagttg acgaatactt
     1861 tcttgcgaat ctttatcgtc aggattttgt tgtaaacgtt ccagatactc agcttttgtg
     1921 gcttcaattt tcttgatgtt agtatttgaa gcatttaact gatcttctag cttatttaca
     1981 acattattct tacttgtaac tgctacaact tttgaataat caatatcgcc atctttggta
     2041 agaaagcttc cttgatagac attagttgaa tctgaatatg gatttattct gaaattgata
     2101 ttatcaggag tattaacagg ttttgctttt ttcccaaaag aaattactcc ttcattatta
     2161 acatcattca tagaatattg agaaagtgct tttgtaactg aatctgcttc attcacattg
     2221 actttggtat catcgtcagc tgtatcatca ccagaaatta gtgatacttg gtttgcaata
     2281 aaaacttgat aagctctttc ctctaattta ccttttagga ataagaaagc atccccatca
     2341 gtacttaaca tcccatagct catttcaagc ttcttaggac gatctttttc ttttgttgcc
     2401 atatataagt tatagttttt tccttgcgta gataacaact ttctagcgtt ttgggagtaa
     2461 ccaagtttta ctactgttac atctctattt tgatctgtcc acacatctga aatagtgatt
     2521 tctgctcctg atcgagcaaa ctccaatgaa gaccctatag gtgttgtatt gttcacgaga
     2581 taataattat agtttcctaa ttgataaatg tagctaccca agcctgttaa taaaaaaagc
     2641 actcctagaa aagttgcaag cataatgcgt aatccgtgac gtgttctctt atccttatca
     2701 ccaagcaacc ctccaataat actgtattga tctttggtag atgttgaatt gacgtttttt
     2761 tccatcagtt tgtctgcccc ctttcataaa agagcaaaca atcagacgtt ttttcggtaa
     2821 ttctaggttc gggattttga atcatttcgg tatattcttc cccattaata tttaacacaa
     2881 aaattatctt cctttcttcg ttcctttctt ttgctcttta ttttccaaat aatcaatgat
     2941 ttttgctatt gtaccaagat agtaattccc ctcagctttg ttttttcgga ttttgctaac
     3001 cattggagta ggtacaatct cttcatctct caattcactg aaagattttt tacctagata
     3061 ttttccaata acagatttta aaaaacgttc aaaatctttc tcgtaagttg ttttcttgcc
     3121 ttttagttca tggttcatat tgtcaacgac ttttgcggtt tcaataattg ttttagttct
     3181 cagcttaata atttcatctg gaattttcaa tcctcgtaat ttcatcgttg tactaactgg
     3241 taacccatgc tttactgcat cagcaatact aaaacttttt agatcatcta gtaagctctc
     3301 catatagtta acaaattcat gccaattaat agcattcatt gtctcacccc ttcatttttt
     3361 taaaatgagg ttcgggcgta acacatttat gttattataa tatcataata acataaataa
     3421 atcaatcagc aaatttagat atcccttaaa gtatcagcaa ttattcaaca agatatttat
     3481 tttagctatt tcagtcatca taaaagacta attagcaata aaataactaa tattatccat
     3541 ttatcagacg aaaagaaaaa acttatctaa ttactctata ttaactttta acaaatagtt
     3601 gacttgtcca aaaatgttac cttcaatctc acaaagatat taagatgttt cctaccaaaa
     3661 ctgatatcac cggacttttt attctgtgtt gtccaaaagt gttaccttta gtcgatcaac
     3721 aaattaaaaa aaattgtttt tttcttgact tgtccaaaag tgttacccat agttgttaaa
     3781 atttgtatag attagccatt ctttcgaata ttatttagac tgaaacttgc ttacaaaaaa
     3841 acttcttgca tttattgtcc aaaagtgtta cccatacttt tttaaagaag caggttgtat
     3901 taaaattaca acctgcttct tctcaatctt cttctaacca attatgcatt gaaatggttg
     3961 gcaagtttga ttctggctca ctaaaagtga atctgagccg atcaactctt cttcctttct
     4021 ttgcatagat tttttcataa tcaaaagttt caaatacctg tattccgttt tcatcaggag
     4081 ctaaaaattc ttctttcgcc tgctctagta ttcttttgtc aatatttgtc attctataac
     4141 ttgaaggtat acttaatgct cttctaaaat cttcaacttt gactacccaa taaccatttc
     4201 gccactcttt atttctgtat tgcatgagat gacgatacaa ttcttttgtg tagctacttc
     4261 ttagttttgt aaaattatca agttcaaaac gagtaaagtt tttgccaatt gagtttaaga
     4321 taaattcaaa ttcctcattt gccttaattg taactgtttc cttctgttta tctatccgga
     4381 atttttgaaa aagccacagc tgttcataga cgtctgaatc ttcaaaacgg taggttaaag
     4441 tacctagttt gtcactcaaa gcttttaaca cagaataaaa actgtccttt tctcttctgt
     4501 cgtatttgac caaataacga aagaggttga aatcaaaagt tacttctgct gttccttgac
     4561 gtttcatttt tgataaaata gcccaaaaca catccatttc aacaggctta aatcctcgca
     4621 aagctgttgt attcagacca ttgttataac gcacaattgc atttgtttgt tctgatacta
     4681 aatctacatc actatcaata atcttttcgg taacttcttg agacattgat tactctccaa
     4741 tcttcttttt gcgctccttt aatcatgtta atctggtact aattaaagga aacaattttt
     4801 tctgcttgtt acctaaatta aatcacaact aaaggtaaca gtcaacaaag atttgttact
     4861 aatctaattt tctatttttt cgccttccaa atcaagaaaa ccgaaggtaa cgtcttatcc
     4921 tagtgtgata cctcaaatgt ccacttttgt tacctttgct gtccaaaagt gttaccctta
     4981 ctgtccactt ttgttacctt tactgtccaa aagtgttacc tttatacccc tttaatactt
     5041 actctcccaa gagattaagc ctcccctaac agatatatta acagatattt aacagatata
     5101 ttaacagata tttaataaag gaggattact cctctcttat ttcaaaatct taaaaataaa
     5161 aaatcttaaa aaaaactaac actatctagg agtttatttt gcttgtgtaa ctttgaataa
     5221 ttgaaaaaaa ggaaaaggag gaagcttca
//
LOCUS       DACDKJ010000113         5216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDKJ010000113 DACDKJ010000000
VERSION     DACDKJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.118"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..248)
                     /locus_tag="IV328_002791"
     CDS             complement(<1..248)
                     /locus_tag="IV328_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8938855.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIY"
     gene            complement(364..1278)
                     /locus_tag="IV328_002792"
     CDS             complement(364..1278)
                     /locus_tag="IV328_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8938856.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(1369..1797)
                     /locus_tag="IV328_002793"
     CDS             complement(1369..1797)
                     /locus_tag="IV328_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8938857.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(1802..2176)
                     /locus_tag="IV328_002794"
     CDS             complement(1802..2176)
                     /locus_tag="IV328_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8938858.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            2462..2647
                     /locus_tag="IV328_002795"
     CDS             2462..2647
                     /locus_tag="IV328_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8938859.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            2659..2907
                     /locus_tag="IV328_002796"
     CDS             2659..2907
                     /locus_tag="IV328_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938860.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            2938..3117
                     /locus_tag="IV328_002797"
     CDS             2938..3117
                     /locus_tag="IV328_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938861.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3160..3630)
                     /locus_tag="IV328_002798"
     CDS             complement(3160..3630)
                     /locus_tag="IV328_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938862.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3717..4415
                     /locus_tag="IV328_002799"
     CDS             3717..4415
                     /locus_tag="IV328_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8938863.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4593..4934
                     /locus_tag="IV328_002800"
     CDS             4593..4934
                     /locus_tag="IV328_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938864.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4927..>5216
                     /locus_tag="IV328_002801"
     CDS             4927..>5216
                     /locus_tag="IV328_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8938865.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1738 a          817 c          942 g         1719 t
ORIGIN      
        1 ttgtaaataa ctaaccattc ttcgacaagc tcatgaaatg ttagatcagg cttctgaagt
       61 gttttttcgt ttaattttat atcaattttt ttatttaact ctagcaatgc cactttctga
      121 gtttctcgag atttattttt ataagtaatg ctcacttttt ttctttttct agtttttgga
      181 tcaatatatc gctcattata tttatatact ttttttccgt ttttatcagt tttggtttca
      241 atccacattg ttttcatctc ctatttgttg ctataatagg aatggataag taagccaatt
      301 atagcaggtt tatttttcat cacgtccaca aactttggcg agggaggggg cgtgtttttt
      361 tgtttagtag ctacctttcc atgaagtatc tgcgaagaac acttcaaatg ttgttccatc
      421 atttttcact tcaaaatatg ctgttccttg tgcggattta cccggttgga tggtttccga
      481 aaagtaatct ctactagcta aatcagattt aatatctgat gaatcataaa attctaaata
      541 acttgaactt gcatcaaaag gtgtagtccc tgtgttttct acaagaaaat ctacctttgc
      601 gtaaaatagg ccttctggtt tatagaaatc atcaccgtaa tcttttgata cagactggac
      661 agttacgtta atagagttac cattttcaga agtaaaagga actgcttctc ctatactatg
      721 tgcaactgat gccgtctggg gttctgaaga cgaagttgta gttgtagttg tagtagagct
      781 tgaatctttg gattttccat ctttatctaa gaaagttcgt tgattataat atacaacctg
      841 ttcagttttt ggactaaccc aaattaacat tgtttcatca tcagaatcag ttgaataagt
      901 atattggagc atctctaatt tttttatatc ttttgcacta tttccatgtt caattaattc
      961 agagtgctct ttataataat ctccttgtgc atcctttgaa atagatacaa ttaattcatc
     1021 ccgtgtgata gcattgtcta attctttgta aacaaaatca tttgtccatt tacgtaacgg
     1081 tgaaccaagt gtctgataaa cttgagttaa atcatcacca acttttattt tatgaacgtc
     1141 tgatgaagtt agtttttttg tttcatattc agatgttgat gaaacagtat tttctttagt
     1201 gctagcagaa ttaggactag tggagcatcc gcttaatacc aagcctaaaa aaagcaaccc
     1261 aaaaacaatt tttttcattt ttaattcctc acttctatga tatgattttt ttgtaggatc
     1321 ttagaaataa ggtttttagt ccgtgttgca gcacggactt tttttacatt atataagaaa
     1381 gcgataagcg ctttctggaa gtccgtaaag attctttaat tcctcaattc gtttaggata
     1441 ttgatcattg tcttctttgt atagagaaac aatgagatta gcagcaaagc aattagcttc
     1501 gctttcagat ttgcttctag atgttcttgt tgatacatag taactggata agccacgatg
     1561 aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat ctttcctcag agtctctcag
     1621 ttcatcattt aagaagatta ttgcacgacc tctaatttct tgaaactgtc ctttgggatt
     1681 ctcgataaaa ggaacgtatt gaattttaat gcccatcttt tcacaaatat aaaaaggatt
     1741 agcggactgg tatttccgct tcaactcctc gactaaatta atcgtatcca tctccataag
     1801 ctcacatctt tttgcctttt tctttgtctt ctttcacaat atcccagaag gttccaataa
     1861 ggatatcttt tacgcgttgg acctgttcag gcgtcagtgt ttctccgcca taagacatat
     1921 tgacatttga atctagtaat ttatcaagtt caactacttc atcttttgtt gcccagtcag
     1981 gaacttggtt tctaccgagt aaataatcag tagttacatt aaaataatct gctacaagtt
     2041 gtagtctttt agtactagga gttctatttt tccattgata aatcgtgttt ttaggtatat
     2101 ttaattcttc ttccaattga gttatactta tgcctctttt atgagctaat tcctttattc
     2161 tatctagtaa attcattttc gcacctcaca agctacgaaa acactaataa aaaaattagt
     2221 taaaggtgtt gacaactaat aaaagtgtta gtatactgtt ttcgtaagct aaaatattag
     2281 ctaaaaagac ttgataacta ataaacactt cacggtcggc aaactctgaa atgtaaatta
     2341 ctaggcattt ctgtgtctta tttagctatg tctatatact aataaaaata ttagttattg
     2401 tcaacgaata ttagctaatt ttttagctta cagattattt ttttagaaag gagctatttt
     2461 tatgtctgag aatttagact taaaaattcg agcggagatg agaaaaagaa gaatgacttt
     2521 caaagaacta gctgcgctta tcggtatttc aggagcttat ttatcagata ttctaaacgg
     2581 caatcgtgat ggaaagaaag cacaacagca tatcgaaaca gtgaaaaaaa tattggacat
     2641 ccgatagggg ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa aaaatacaat
     2701 tattctccat ctacttatca acgtcgaatg tcggaactaa aaaatacagc aattttctca
     2761 gcggcgtatg aacgggtcac aggacaagaa gtttggatca atacagaatt atacgataaa
     2821 tttttgtctt tcaaatccta taacaggtta cgcacaagaa aggtaacacc taaagaattt
     2881 atcgagaagc atttagttga tttataaaaa acagaacatt ttgagaggtg aaggttaatg
     2941 ggtaaattca acagagcatt ggtattcagc gcaccgctaa tcatctacgc tttaggactt
     3001 tggggaagca ggcaagcgtt gataggaacg attgtttaca tggtttggat ttttatgggg
     3061 cttgatgaag ctgaagctga gtacagagcg aaaaagccaa ccgagggggc tgactaaaaa
     3121 tgaatagaaa agagaaacta gaatgtatat tactattact cagtttaatt ctttcactaa
     3181 tttctctatt gggaagtttg tatttttgat atcaacaatt ttgtggattt gggttctatt
     3241 tagagtaaat atcatttttg tttggtgatt aaggagttta ttaaaattaa ttttccctac
     3301 ttcaaatgca aggtaacaag atatagcact tttaggagga atatttagcg gtacagcttc
     3361 agagtatatt ctattatcat cgactgatcc tcctttagca attaatttct taaatggagt
     3421 agctgaccat ctgcggttta agttagcgga tgaaagttct aaattaacta acatagctgg
     3481 ttctgttgaa tagttagaaa tgattacttt tgtataaatc atatctgcag cgaagtaaga
     3541 agcattaagt tcaacttcta gttgagggcg cattcctttt atttttatcc ctgttaaaaa
     3601 cgtactcaat ataaaactta ttattgacat ccattgaaaa attgttaagt gaaaaaaatt
     3661 caaaataaac accaccagtt tttaactaaa ttataccaaa aaggagagaa gaaataatgc
     3721 aagaattagt aattttgaaa aataaagaag ctgtgactac gagcttacaa gtggcagaaa
     3781 gcttcgagaa aaaacatcaa catgttttaa gagatattga tgcactaaaa aaagatgtgt
     3841 ccaattttgg acagatgttt gtagaaggta atgaaccaga ttcatatggc agaaatcgac
     3901 gagttttctt cattagtaga gatggatttt tcttgctggc tatggggttt acaggaaaga
     3961 aagctatcta cttcaaacaa aaatacattg aagcattcaa cgaaatggaa gatgttattc
     4021 gcaagaatac tgttcctcaa acaattgaag atatgatgat ctatcaatta gaagaaatga
     4081 aagatgttaa aaaagatgtt tccatgctta aagatactat gcgaattagc ggacaacaag
     4141 agtttgaaat taagcaaaaa ggaaatatga aagttatggg agttctaggg ggaaaagaaa
     4201 gccgagctta tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac
     4261 gtaccttttc aatcccaaga tatggcgagt tacctcgtaa gagattcgat gatgctgttt
     4321 catttattga aatgtggttg ccagaaactg cgatccgtat ggaaattgat caactgaaca
     4381 gacaacagag actttttggt gatgacaatg aatagagctg aagcgctaag aatagggacg
     4441 gcaattgcta attgctggtg gaaatactat aaaccaatca tcctaagcca acaacatatt
     4501 gacaagcaaa aagcatggca acaaataaaa aagtgactcc gccggcaagc aaagagtcac
     4561 aaagaaaaca catcataagg agattttagc atatggaaaa agaactttcc actctagatc
     4621 aatatttgat tgatcctgat tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa
     4681 tcagacgaaa tcttttgacg aatgaagaac tagcttggga tcaagatgat ttaggcaacc
     4741 atgtaactat ttgggatcat gtttatctta tccatctatc gaagcattcg aataaacctg
     4801 aatatattta cgtcatcgaa gatggcttga ttgatgcgct agaagagtac gacagagata
     4861 acttgattga tatctcttat tacggaccag gtaagaaata cattgctgaa atggaggcag
     4921 aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag tcgtaagtta
     4981 aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca cgcgtgggag
     5041 attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtacaa acactacagg
     5101 gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt tcttatggat
     5161 gaaactggga cttatgtatc tgtctcagta acggttaaag gacacacgga aaccga
//
LOCUS       DACDKJ010000114         5038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDKJ010000114 DACDKJ010000000
VERSION     DACDKJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.119"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..804
                     /locus_tag="IV328_002802"
     CDS             <1..804
                     /locus_tag="IV328_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8938866.1"
                     /translation="TKVLTTGNFKGGVGKTTNAVLTAYTLSNKGYKVLVVDLDPQANA
                     TELLFATMTNVYKIKPEFKETLFVSIQNNKISNSLISVKENLDLLPSFTDLEKYIDYL
                     AELYDDDYSKDTHFSNLLNEIKENYDFIIIDVPPQLNKFTNSALVASDYVIVILQTQE
                     RSLKGAEKYIEHLIQLNNDYGTEIDILGLLPVLVQNGNDLDLDIIEDAESLFGKSNIF
                     NTKIKTMQRLKRYDRTGITDNPKDIHDKRVQQVYQSVSDEVIQRINLLG"
     gene            818..1180
                     /locus_tag="IV328_002803"
     CDS             818..1180
                     /locus_tag="IV328_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938867.1"
                     /translation="MTNEKNESIGLNAIKRKNKTQRPENIVPENSEALNNTTDSGKIL
                     EQSKKRLTTKDLPKSIRMPLDTHTAISTIATIEDKKIYETLNKIVEFYINNMNPANKK
                     IVKNSIKAVQNIYKDTDN"
     gene            complement(1449..1799)
                     /locus_tag="IV328_002804"
     CDS             complement(1449..1799)
                     /locus_tag="IV328_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938868.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDAENEQRANQNLAKEQMTTE
                     EITEVLNAAVIKNRSVCIQKEERNAEGYYPDDIIGKIFGSDELGIYVGKNKVHYDEIR
                     HIEFYQKHKWSCLE"
     gene            complement(1792..3117)
                     /locus_tag="IV328_002805"
     CDS             complement(1792..3117)
                     /locus_tag="IV328_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8938869.1"
                     /translation="MFDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMKLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQDRIKEELGLIA
                     AVGVGDNPLLAKLALDNEAKNNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIPPEPSKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITNNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIIHASSKLEGARSITRSTLVGGHAGGVGGLKND"
     gene            complement(3389..4636)
                     /locus_tag="IV328_002806"
     CDS             complement(3389..4636)
                     /locus_tag="IV328_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAP8938870.1"
                     /translation="MKNKLTLLICATILSTWAVPTQNAFAEETGNDTTQQTVTSNSSS
                     EEQTAIVNGEEVKLEAPVKEGQSEAEATQAPKEELQQDQMSRFARSAVRTIPMYRMYN
                     PNSGEHFYTQDTGERDNLRRIGWNYEGIGWQAPTSGDPVYRLYNRYNGEHFYTLNAKE
                     RDNITKQGWTYEGIGWYSYKGANGIPVSRLYNKRVNWHHYTLDEHEKNVISKQGWNYE
                     GVGWYAVGNGQPSNDDYKLLGVKNYNQYALGAPSGCEGASLLQALQYKGKITNWSLTQ
                     FLNTIPKSPNGNPNNGFVGSPFVENSWTYSAIYPAPLATWGQKYGNVQNISGSSMNTL
                     LNEVKNGNPVVAWVTINFQPIRWGNWSFGVAANNNHAVTLDGYNKGSNQVHVSDPISG
                     SYWLNRTTFENIYNARKYAVVVR"
     gene            complement(4904..>5038)
                     /locus_tag="IV328_002807"
     CDS             complement(4904..>5038)
                     /locus_tag="IV328_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8938871.1"
                     /translation="TGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM
                     "
BASE COUNT         1586 a          946 c          751 g         1755 t
ORIGIN      
        1 acaaaagtat taaccacagg gaattttaaa ggtggtgttg gtaaaacaac taatgctgtg
       61 ttaacagctt atacattatc taacaagggt tacaaagtct tagttgttga tcttgatccg
      121 caagctaatg ctacagagct attatttgcg actatgacca atgtatacaa aataaagcct
      181 gaatttaaag aaacattatt tgtttctatt caaaataata aaatttctaa ttcactaatc
      241 tcagtgaaag aaaatttaga tctattacca tcatttactg atttagaaaa gtatattgat
      301 tatttagcgg aattatatga cgatgattat tctaaagata cgcatttctc aaatttatta
      361 aatgaaatta aagagaacta tgattttatt atcattgacg taccaccaca attaaataaa
      421 tttactaatt ctgctttagt cgcaagtgac tatgttatcg taattctgca aacacaggaa
      481 cgctcgttaa aaggagctga aaaatatata gaacatttaa ttcaattaaa caatgattat
      541 ggaacagaaa tagatatttt aggcttgcta ccggtactag ttcaaaacgg taatgattta
      601 gacctcgata ttattgaaga cgcagagtca ttatttggaa aatcgaatat tttcaataca
      661 aaaatcaaaa caatgcagag attgaaaagg tatgatagaa ctggcataac cgataaccca
      721 aaagacattc atgataaaag agtccaacaa gtttatcaaa gtgttagtga cgaagtaata
      781 caacgtatta atcttttagg ataaggagct tttcaaaatg actaatgaaa aaaatgaatc
      841 aattggttta aatgccataa aaagaaaaaa taaaacacaa cgacctgaaa atattgttcc
      901 agaaaattct gaagccttga ataatacaac tgattcaggc aaaattctag aacaatctaa
      961 aaaacgactc acaactaaag atttacctaa atctataaga atgcctctag atactcacac
     1021 tgcaatcagc acgattgcga caattgaaga taaaaaaata tatgaaacgt taaataaaat
     1081 tgtagaattt tatataaaca acatgaatcc agcaaataaa aaaattgtaa aaaatagtat
     1141 aaaagcagtg caaaacatct ataaagatac cgataattaa tttataaaat tataaaatta
     1201 tgaatttata attttataaa actataaaaa ggatagaatt ttttctatcc tttttattta
     1261 taccgaaaag tgagaatcac tgtaattcaa tatatgatat tcaaaattta agtatccacc
     1321 agctattaat acgtaaaact taaaacagtc gattcctatt tcattaatta gttgttgacc
     1381 atatccccag atttcatctg aaagattttc aagtgttata tacttaagct catattgctt
     1441 tataatgttc attctaaaca gctccattta tgtttctggt aaaattcaat atgacgtatt
     1501 tcatcgtagt gaactttgtt ttttccgaca tagattccta gctcatcact tccaaaaatt
     1561 ttaccgataa tatcatctgg atagtatcct tcagcatttc gttcttcttt ttgaatacat
     1621 actgatctat tttttatgac tgcagcgttc aatacttctg taatctcttc agtcgtcatt
     1681 tgttcttttg ctaagttttg attggctcgc tgttcattct cagcatcgat ttcttcagta
     1741 tgatctgata aatagaatcc tgcccacttt aaacgtccac gatcattgta atcaatcatt
     1801 tttaagccct cctacgccgc ctgcatgacc accaactaac gtgcttcttg taatacttct
     1861 agcaccttca agcttagaag aagcgtgtat aattgacgta aaaccatatt tttgtctaat
     1921 tttatctatt attttatcta gtttttcgtc atcaatctgt ttttgcggat cactaaacaa
     1981 atctaactga agcgaatcag tgtatactag cttgctatag gttattccga cgtgtcggac
     2041 ttcttgcccc tcatagtatt tattaaataa gaacagacaa tagtcttgta gctctcttgt
     2101 attatttgtg ataggtatct tcatctggtg tgaaaaccct ggtctcgttt ctaagatcga
     2161 cgttccaatg tgtaaatgaa cacaacctgt tttacagtgg tgttgcctaa tacggattgc
     2221 tacttgctca gccatttctt ttacgaccag ttcgatttca tttcttctag tataatctcg
     2281 tggtaacact tgtgaattgc cataagattt ttcctttgac ggttctggtg ggattgctat
     2341 gtcagttcga tcaataccat ttgcatggaa gtacagctgt aacccaatca cgcctaaacg
     2401 gctcttaatg gtgtatggat tccaattagc taaatctttg atactcatga tccccatttg
     2461 gttgagcctt tttttcattc gagaaccgat accccaaaag tctgtcattt caggaatatt
     2521 ccatacttta tcaggaacat tttgatacgt ccattcggca ataaatcctt cattgttctt
     2581 cgcttcgttg tctaaggcaa gtttggctaa caagggatta tctccgacac ctactgcagc
     2641 aatcaatcct agctcttctt taatacggtc ttggatcatt tgagcgagct tcttccttcg
     2701 ctgacttcgt gttccttcag tcgtaaaaag attcagtgat cgggtcactt ttaagattga
     2761 ttcatcgatc gagtaaatca gtagatcttc atcagccaca tatcttctga aaatattatt
     2821 tacctgcata tttcgcttga tatatagttt catacgtggt ggaacaacgt gtagtgtttt
     2881 aggaaatggt tgtggtaaat cacgtggtct actcacattt gtaataccat accgcttttt
     2941 tgcttcagga gaagaagcta atatcaatcc tgaaccagta ttgtcagctc gactcataac
     3001 aacaagttct gttgttaatg gatctaaatt tctttctata cattcgacac tcgcataaaa
     3061 agacttcatg tcgattagaa aataatcatt tactggttct ttcgaataat caaacatgaa
     3121 taacacccct tctttttaca ttatacaaac gtatgttcga tattgtaaag aagaaatagc
     3181 taaacacaca aaaaaagaag cgtaggtaaa ctcttaccct gcttcttctt ttgatttgta
     3241 tgatcaactc attgtatcac tttattaaat aatttgaaaa tttaacaaaa actctcactc
     3301 taaatgagtg agagactgca aattagagta cggaattttt gggagttccg ttccttaata
     3361 ttaacaaatg atcttgaagc atacaatgtt atctgactac aactgcatat tttcgtgcgt
     3421 tgtaaatatt ttcaaatgtt gttcgattca gccaataaga accactaatt ggatcagata
     3481 catgtacttg attacttcct ttattgtatc catcaagtgt cactgcgtga ttattattag
     3541 ctgctacacc aaaactccaa tttccccaac gaattggttg gaaattgata gttacccatg
     3601 caaccacagg atttccgttc tttacttcat taagcaatgt attcatagaa ctacctgaaa
     3661 tattctgcac atttccatat ttttgccccc aagttgctaa tggagcagga taaatagctg
     3721 agtaagtcca agaattttca acgaatggac taccaacaaa cccattgttt ggattaccat
     3781 ttggagattt aggaatcgtg tttaaaaatt gagttaaact ccaatttgtg atctttcctt
     3841 tatactgtaa tgcttgtagc aatgaagccc cttcacagcc acttggagcg cctaaagcat
     3901 attgattgta attctttacg ccaagaagct tatagtcgtc attgcttggt tgtccattgc
     3961 cgactgcgta ccaacctacg ccttcataat tccaaccttg cttactaatc acgtttttct
     4021 catgttcatc tagtgtgtag tggtgccaat ttactcgttt attatataag cgactaactg
     4081 gaataccatt agcaccttta taagagtacc agccaattcc ttcatacgtc cacccttgtt
     4141 ttgtgatatt atcacgttct ttggcattta aagtatagaa atgttcccca ttatagcgat
     4201 tatataagcg ataaactgga tcgccacttg ttggagcttg ccaaccaata ccttcgtaat
     4261 tccaaccaat tcttcttaga ttatctcgtt ctccagtatc ttgagtgtag aagtgttctc
     4321 cgctatttgg attatacatg cgatacatag gtattgttct tactgcacta cgagcaaacc
     4381 gactcatttg atcttgttgt aactcttctt ttggtgcttg agtcgcttca gcttcagact
     4441 gtccttcttt cactggcgct tctaatttta cttcttctcc atttacaatt gctgtttgct
     4501 cttcgctaga actattgcta gtcactgttt gctgcgtagt atcattccct gtttcttctg
     4561 caaaagcatt ttgtgtaggg actgcccacg ttgacaaaat ggttgcacaa attaataatg
     4621 tcagtttatt cttcattttt atttagtcct ccatctcttt attaaaagtg taacgatcgt
     4681 ccttttaggg acttgatatt gacaataaaa cgatatttgc ttttctttaa atatttactt
     4741 tgcattaatg tacaacgtac gactctcact aaaacacagt tgtcttttag catttgtaaa
     4801 acttgaactt caaaagaacc ttccaaacta ggaacttcgc aataataaat tttaccgatt
     4861 tctatttctt gcattattta cgcttctttc taactggaac ttattacatt cctaaatctc
     4921 tttttactcg ataaacagtt tttcttgtaa cccctgcatt ctttgctatt ttagaaatag
     4981 tttctttttt cttcagacca tcaacaattt catcgtaaat caatttgttt tttcctgt
//
LOCUS       DACDKJ010000115         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDKJ010000115 DACDKJ010000000
VERSION     DACDKJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.121"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IV328_002808"
     CDS             564..989
                     /locus_tag="IV328_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938872.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IV328_002809"
     CDS             2141..3118
                     /locus_tag="IV328_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8938873.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IV328_002810"
     CDS             3128..3625
                     /locus_tag="IV328_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8938874.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IV328_002811"
     CDS             4001..4282
                     /locus_tag="IV328_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938875.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IV328_002812"
     CDS             4284..4700
                     /locus_tag="IV328_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938876.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDKJ010000116         4754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDKJ010000116 DACDKJ010000000
VERSION     DACDKJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.122"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..1205
                     /locus_tag="IV328_002813"
     CDS             15..1205
                     /locus_tag="IV328_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP8938877.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1339..3528
                     /locus_tag="IV328_002814"
     CDS             1339..3528
                     /locus_tag="IV328_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8938878.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3525..>4754
                     /locus_tag="IV328_002815"
     CDS             3525..>4754
                     /locus_tag="IV328_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8938879.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT         1706 a          728 c          860 g         1460 t
ORIGIN      
        1 gagaggactt ccctatgaac gattttacta cagaaattct aaagactcta gcgaacaaag
       61 gcgatttgaa tgaattattc cgtgtccatt tggagaaagc agtcaatacg cttctcaaaa
      121 cggagttaac ggctttccta gattacgaaa agtatgatcg cattggtttt aacacgggta
      181 attctcgtaa cggctcctat gaccgtacgg tcaagaccga gtatggggaa cttcatctcc
      241 agattccgcg cgaccgcaat ggtgagttca agcaacagac tgttcctgct tatagacgga
      301 cgaatgacac gttagaggag accgtcattc acctcttccg aaaaggtatt accatgtcgg
      361 aaatcgcaga cttgattgag aaaatgtatg ggcattacta cacgccccaa accatgtcca
      421 atataacaaa atcatttaca gaagaggtaa cggcgtttaa tgggcgggag cttcatgacc
      481 gttatgctgc tatttatatg gacgcaacgt atattccgtt aaagcggaaa accgtcgcca
      541 aggaagctat tcatatcgca gttggcattc gcccggacgg atcaaaggaa gtattgagct
      601 atgcgattgc accgactgaa tccatcacga tttgggagga aattttattg gaccttcaag
      661 agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg atggtaggag
      721 cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac gtttcccgta
      781 atatcagtca taaagtgcgt gtcgatgatc gtaaggaagt ctgtgatgat tttaaaatgg
      841 tgtatcaagc ctcatctaaa gaggtggcgt tggaagcacg tggtactttt gcggaaaaat
      901 ggaaaaccag ctatccaaaa gtggttgaat cgattctttc gaacgatcac ttgctcactt
      961 tctatgattt ccccttggcc atacgcaaga gtatttattc tacgaacttg attgaatcct
     1021 ttaataaaca aatcaagaaa tacagccacc gcaaggaaca gttccaaaat gaagagtcga
     1081 tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta ggtcgcagtc
     1141 ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat gctaagccaa ctgattgaga
     1201 attagaaaca atctgcagta gggaagaatc atttacacaa aattattgac gctcccttat
     1261 aatgagaact tttactaaca taatttatga taaacttact atataatcat attaattaaa
     1321 tagttaggag atctgcttat gtttatatct actcttcaaa ttcgaaattt tagaaattat
     1381 gaatccgaaa tctttcgatt cgataatgag acaaatacta ttataggtga gaatgattca
     1441 gggaaaacca atgctcttac tgcattgaga atcttattag atgatagtta ttactatagt
     1501 agtaaaacac taaaagaatc agattttttt catggaattc aaaatgggtg gcaagggcat
     1561 tggattatta tctctgcgac atttgaagga atcagcgaag aagaatttga caatgagatt
     1621 tgtgcttcca tttctttaaa ttctgaaagt caaacaatct tagaagaatt gatttcaaat
     1681 gcagataaag gtgtaggtag tatttctcta tttattagac ctaacaaagc tattagaaag
     1741 caattatttg atatttccac tgaattagac tctcatcaat ttaacgaatt tagaaattct
     1801 attcgtctat ccgactatga attttactac acatctaaat caaatctaaa tttctgtatc
     1861 aatgaaaatt atgataaatt ggtaggaaga ctcaatgaca atatagctag taatcctgaa
     1921 gaagacgatg aagctctgtt gggctcaaga attgacatgt cagatatttt caagcatatt
     1981 tcggttgttt atgttgacgc tcttcgagat gttcttagag aaatgaaaaa caatcgaaac
     2041 ccagtcaaaa gaatcatgga aacaattgaa tcaaaaatat catcagataa tgttgagtca
     2101 ttaaaaacaa ttatacagca attaaatgag actattacca gtgttcctga aattaggaaa
     2161 attggcgaaa atattaatcg gcaactaaat agcataattg gttcagtgta ttctcctaat
     2221 cttcttcttt cctcaactat gtcagatgat atgggttctt tagcaaaatt tctttcaatg
     2281 aagccagaac aaaatatcga cttagactta ctagggttag gacatctaaa tatgatttat
     2341 ttagctttga aaatagtcga atttgaagcg tgtcgttcaa gagaactact taatattatg
     2401 ttgattgaag aaccagaagc tcatattcat catcatattc aaaaaactct gttcgaagga
     2461 ttaaatcttc aaaaaaacta tactcagatt ctcatgacca cacattctgt acatttagct
     2521 gaagtgtctg aaatatctcg gatgaatgta ttaaaatcgt ttaatggcaa atctatcgtt
     2581 atgaatccag caaataatct gaactcattt gctaaagaaa agttaaaaaa gaacaatcta
     2641 aatctaataa acgcagtaga gagatattta gatgttaaaa gaaatggatt gcttttttct
     2701 aaaggtgtta tcttagttga aggagatgct gaagaaatac ttattcctca aatagtaaaa
     2761 aaattatttg gtataaactt agacgaactt ggaataggtt tgattaatgt tggtagtact
     2821 tcgtttgaat atatcgctag cttgtttgac gatgatagaa tacagagaaa gtgtgctatt
     2881 gtaactgatt tagatgaaca ggcaatccca tcagaacatc gattgtataa aagcactgca
     2941 caagagaaag gtcaagcaag aaaagaaaaa ttatcctccc tttttaatga aaacaattgg
     3001 gttaattgtt tttatgctaa tacaacattt gaaattgagt ttttagatgt gaataaagaa
     3061 aatattctaa cttatgtcag tccaattata gatgacgcat tcaaagataa aaaaacaata
     3121 actgcttata aagaagaaat acaaagtgaa caatatttag aaagaaattt agcgatgtta
     3181 aaactagcca atcatgttgg taaggggtgg tttgcaattg agttaggtga aaaaattgac
     3241 tttttaatca aaattcctaa gtatattctt aatgctattg cttttgcatg tcaggaaata
     3301 gtagatctct caatttataa aaaaattcta ctccatacaa tcgacttgta cgaggaaact
     3361 acagaattgt cagctttatc agctagttta aaaacaagtg atacagctga tttgaatata
     3421 gcaataattt ctttattaga gtactttggt aaagatgagg atgttgtgta tctaatcaat
     3481 cagatagaaa attacacaaa aatactttat gctgaggttt caaaatgaca aatatcttcg
     3541 aaaaaatcgt aaatgattta aatttagaac aaaaagatgt tgtggaaagt gaagataacc
     3601 tttatgtaaa tgcatgtcct gggagtggga aaacaagggt acttacaagg aaaattgctt
     3661 accaagcaat taaacacgat ggatcactaa aaagaataat tgctatcaca tacactaata
     3721 gagctgctga agaaattaag gaacgcctaa gtttacttag tatagatgat gatattaata
     3781 tttgggtggg gacaatacat cagttttgtc ttgaatttat tatttatcct tttaaaatga
     3841 atctctcaag agtatcaaaa ggatttacaa ttattgatga ttatactcaa agagcttacg
     3901 tcagccaaat taataatatg ctgaatttaa atctcaaaac gtatgaactg aacaaaattg
     3961 atttgtcact ttctcatgat tttaaaatta atgaagttca gtatactcaa gtttctgaaa
     4021 tgtatcacac tatgttggaa aaaaataaag aaattgattt tgatatgatt ttactagtag
     4081 cgtataaaat tttaactgta aacagtaacg ttgcacgtaa tattgctagt aatataaggg
     4141 cgatttatgt ggatgaattt caagatacta gagaattaca atacaatgta atagctaaat
     4201 tacttcaaaa caaccctcaa attcagtcaa tgtttgttgg agatattgac caagcaattt
     4261 atggatcatt agatggtata gctaaatcag ttgaagaact agaaatgcta actagtcaca
     4321 cttttcaatc aaagacgcta catggctgtt acaggtctaa tcagagatta gtagacttct
     4381 actctaattt tcagagttgt ccttacgaga ttgaaagcag agggaataat aaaaacgacc
     4441 gtggttttat aagttatgat tgcaaagtta ctaaggagga tttacccaac ataataaaaa
     4501 atataataaa ggaaaagatt gaaagcggca ttcatgaaaa agatatttgt gtacttgctc
     4561 ctcaacacta tcctctgttt tcactgggtg agtatttaaa gaaagaatta ccgtattgca
     4621 attttgatgc tataaatata tcaccaatta aggtaaataa tcatagttta tttttcaagt
     4681 tagcaatttt gtactttact gaatcaggtg aaaaggttcg aagaagaaag ctaattgcaa
     4741 acgatatttt aatt
//
LOCUS       DACDKJ010000117         4713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDKJ010000117 DACDKJ010000000
VERSION     DACDKJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.123"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(213..443)
                     /locus_tag="IV328_002816"
     CDS             complement(213..443)
                     /locus_tag="IV328_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8938880.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="IV328_002817"
     CDS             complement(673..1491)
                     /locus_tag="IV328_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8938881.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="IV328_002818"
     CDS             1652..2341
                     /locus_tag="IV328_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8938882.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="IV328_002819"
     CDS             2355..3857
                     /locus_tag="IV328_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8938883.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="IV328_002820"
     CDS             3870..4346
                     /locus_tag="IV328_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8938884.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="IV328_002821"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="IV328_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1590 a          673 c          830 g         1620 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACDKJ010000118         4710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDKJ010000118 DACDKJ010000000
VERSION     DACDKJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.124"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IV328_002822"
                     /pseudo
     CDS             106..357
                     /locus_tag="IV328_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IV328_002823"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IV328_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8938885.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IV328_002824"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IV328_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IV328_002825"
     CDS             1786..1869
                     /locus_tag="IV328_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8938886.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IV328_002826"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IV328_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8938887.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IV328_002827"
     CDS             complement(2994..3857)
                     /locus_tag="IV328_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8938888.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IV328_002828"
     CDS             complement(3859..4131)
                     /locus_tag="IV328_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8938889.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IV328_002829"
     CDS             complement(4148..4363)
                     /locus_tag="IV328_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8938890.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="IV328_002830"
     CDS             complement(4455..>4710)
                     /locus_tag="IV328_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP8938891.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DACDKJ010000119         4635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDKJ010000119 DACDKJ010000000
VERSION     DACDKJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.125"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            175..519
                     /locus_tag="IV328_002831"
     CDS             175..519
                     /locus_tag="IV328_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8938892.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="IV328_002832"
     CDS             520..939
                     /locus_tag="IV328_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8938893.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="IV328_002833"
     CDS             complement(1031..1381)
                     /locus_tag="IV328_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938894.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="IV328_002834"
     CDS             complement(1347..2567)
                     /locus_tag="IV328_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8938895.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="IV328_002835"
     CDS             2933..3499
                     /locus_tag="IV328_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8938896.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="IV328_002836"
     tRNA            3644..3731
                     /locus_tag="IV328_002836"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4635
                     /locus_tag="IV328_002837"
     CDS             4065..>4635
                     /locus_tag="IV328_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8938897.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaa
//
LOCUS       DACDKJ010000120         4611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDKJ010000120 DACDKJ010000000
VERSION     DACDKJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.126"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(86..340)
                     /locus_tag="IV328_002838"
     CDS             complement(86..340)
                     /locus_tag="IV328_002838"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8938898.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="IV328_002839"
     CDS             complement(451..705)
                     /locus_tag="IV328_002839"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8938899.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="IV328_002840"
     CDS             complement(898..1173)
                     /locus_tag="IV328_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8938900.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="IV328_002841"
     CDS             complement(1145..2098)
                     /locus_tag="IV328_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8938901.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="IV328_002842"
     CDS             2710..4203
                     /locus_tag="IV328_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8938902.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="IV328_002843"
     CDS             4338..>4611
                     /locus_tag="IV328_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8938903.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DACDKJ010000121         4577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDKJ010000121 DACDKJ010000000
VERSION     DACDKJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.127"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..517
                     /locus_tag="IV328_002844"
     CDS             <1..517
                     /locus_tag="IV328_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8938904.1"
                     /translation="FGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAAR
                     LVLSAAMNSKFGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKL
                     YEELMTTDEIERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFI
                     RIAKENNVVTW"
     gene            529..1533
                     /gene="neuB"
                     /locus_tag="IV328_002845"
     CDS             529..1533
                     /gene="neuB"
                     /locus_tag="IV328_002845"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8938905.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1530..2678
                     /gene="neuC"
                     /locus_tag="IV328_002846"
     CDS             1530..2678
                     /gene="neuC"
                     /locus_tag="IV328_002846"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8938906.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            2651..3328
                     /locus_tag="IV328_002847"
     CDS             2651..3328
                     /locus_tag="IV328_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8938907.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3318..4460
                     /locus_tag="IV328_002848"
     CDS             3318..4460
                     /locus_tag="IV328_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8938908.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4473..>4577
                     /locus_tag="IV328_002849"
     CDS             4473..>4577
                     /locus_tag="IV328_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8938909.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNE"
BASE COUNT         1685 a          594 c          903 g         1395 t
ORIGIN      
        1 gtttggaaat gtcatggggt caagtggatc tgttattcct ctttttagta aacaaataaa
       61 aaacaataaa gatattactt tgacagatgt tgatatgaca cgatttatga tgtcaatacc
      121 ggatgctgca agattagttc tgtctgctgc tatgaattca aaatttggag aaacatatgt
      181 cttaaaaatg ccggcaatca atattttaac tttagctcaa tcattaataa aaatatataa
      241 tgatacacat gatgaaaaat atagaggtga aataaaagtt gtaggtatca ggccaggaga
      301 aaaattatat gaagagctaa tgactacaga cgaaattgaa cgctcatatg aaaatgataa
      361 aatgtatatc attccaccac actatcatgc tactgacgaa ctgtacaaag attatgaaaa
      421 aattaaaatc actgcatact cttcagatgc aacagaatta atgagtggac aggaatttat
      481 tagaatcgca aaagagaata atgtcgtgac ttggtaagga gttttatgat gaaatcagaa
      541 gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa gtctagattt agctttagaa
      601 agtattgata aagctaaaga atgtggagct aacgcggtta aatttcaaac atttaaaacg
      661 aataagctag tttccaaaaa ggctccaatg gcaaaatatc agaaggataa tcttaaagag
      721 gacagttctc aatttgaaat gctaaaaaaa ttagaattat ctcaatctga ttttattaga
      781 ataaaagatt attgtataca aaataaaatt gattttttgt ctactccttt tgatgaagaa
      841 agtgcagact ttctaaaaca aattggtgta caaggattta aaattggctc aggagattta
      901 acaaatttac cttttcttag aaaaatagat atgtatgggt tgccagtttt attatctaca
      961 ggtatggctg atattcatga ggtcagtgag gctgtagatt gtttcataga tagtccagtt
     1021 actattttac attgtacttc aaattatccg gctagttctg aagatattaa tttattggcg
     1081 attaacacat taaaagaaat gttcgggata cccgttgggt actccgatca ttcattggga
     1141 tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg taattgagaa acattttacc
     1201 ttggataagg aactacctgg tccagatcat aaagcttctt tagatcctca ggaatttgca
     1261 gattttgtaa agcatattag aaacactgaa atatttttag gtgatggtgt taagcgagca
     1321 atgccttctg aacttagtac aaaagaagta tcaagaaaaa gcctagtggt cactgagaat
     1381 ttaaatgtgg gagatattct tacagagaaa tctcttacaa taaaacgtcc tggaaatgga
     1441 atagctccta agtttttata tcaatatatt ggcaaaaaag ttaaaagacc aataagtgca
     1501 gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat acttatagta agtggaacac
     1561 gagctgatta tggaatatat aaacctgttg ctgagaattt actacaagat aattgggatg
     1621 tatcatttgt tgtttctgga atgcatttat cccataaata tggatttact aaagaattga
     1681 tcttagctga cggatttaga attagtggag aagttcaaag tttgttttta gagaacaata
     1741 agggaaacat ggctcggtct gtggcattag agataaatgg tttcacaaat atatttgaac
     1801 aaactagccc tgattttgta ttattactag gggatagagg agaaatgttg gcagctgcga
     1861 tagcttgttt gtatcttgga atcaaaacag cacatattca cggaggcgaa gtgtctggta
     1921 ctgtagatga atcaataaga catgccataa cgaaaatagc atctgttcat tttgctgcta
     1981 cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga ttcttggaga gtttttcccg
     2041 tcggagctcc tagaatagat actattttaa ataatagatt acctagtttt agagatataa
     2101 aagaaaaata tcatctaaaa tttgaaaaga aaaaatacgt tttgcaagta tttcatccag
     2161 ttgtaaccga actagaaaat atagaaaaac aaattttagc tttgatcaat tcaatggaac
     2221 aacaatctca accaattatt tgcattttac ctaatagtga tgctggtagc gaaattatca
     2281 gaaaaatgta ccagaaatat ttaaaaacgg atattattta tattacgaat ttagagccgg
     2341 aggaatacct gactatatta aaagagtgca aatttatgat cggaaattct tcttctggga
     2401 taattgaagc tgcatcattc aaaataccag taataaattt aggaaccaga caaaatggac
     2461 gagaacaatc taaaaatatt ataaatgcaa atctaaatga aaatgaaatt gaaactgctt
     2521 tgaataaaat acaagatcct cagtttataa aaagcttgaa aaatgttaag aacatctacg
     2581 gtgacggcaa cagttctaga agaataacaa aaacattgaa tcaaatattg aatgatgggg
     2641 aagatattaa gtggattcaa aaacaaatag cttattaaaa tatactagtg atccatacat
     2701 tattattggg gctggcggac acgcaaaagt cattattgaa accttaagat tagaaaataa
     2761 gttaatatat ggattttttg atgataagca aaataaaatg caagatctga aatatcttgg
     2821 gaaaataaac gaattgaata gctgggcgga tagcaaaaaa aaaatctata aatttattgt
     2881 agctattggt gactgtgtag taagaaaaaa gattataaaa aaattgaata tgccaagtga
     2941 gcaatatgga atagctgttc atccaagtgc tatagtggca aaagaaattt caattggtta
     3001 tggaactgtt atttttgcga atgctgtaat taacccagat gctgtaattg gagagcatgc
     3061 tataataaac acaggtagca ttgttgagca tgattgtaga attaataact atgttcattt
     3121 atctccaggt gtatgcttag cgggtggagt acatgtaggt gtaggaactc aaataggaat
     3181 tggttctcaa tgtatccaga tgaagaaaat tggatcttgg tgtatgatag gcgcagggag
     3241 cactattgtt aaggatattc cctcacattc cttagcttat ggcaatccag ctaaaattaa
     3301 gaaagaagga attaattttg aattctagaa ttttattatc aagtcctcat atgggaggaa
     3361 cagaagaaaa atatgttgct aaggctttta aagataattg gatagcgcca ttaggtgaaa
     3421 atgtggataa atttgaagaa agtattacaa aatatactgg ttctaagggc ggagcttctg
     3481 cgttaagttc aggtacagct gccatacatc ttgccttaag actgcttaat gttggaaaaa
     3541 acgatattgt ctttgctcct tcatttactt ttatagctac agtaaatcct attttatatc
     3601 aaggggctgt accagtgttc attgactcag aacctaatac atggaatatg agccctgttg
     3661 cactagagag cgcattagaa gaagctgaaa gaaacaacca attacctaag gcaatcatcg
     3721 tagtgaattt atatggtcaa atggctgatt atgaacgaat tttagctatt gcaaaccaat
     3781 ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg ttctacttat aagggaattt
     3841 cgtcaggctt atttgggaca attgggatct attcatttaa cggtaataag atcatcacaa
     3901 cctcaggtgg aggagctcta gtgtctagtg ataaaaaata tgttgagcat tcaagatttt
     3961 tagcaacaca agcaagagat caagcaccgt attatcaaca tagcgaaata ggttataatt
     4021 accgtctaag caatatttct gccggtatcg gacgtggaca aatggaagta ttgaatgata
     4081 gagtagctaa acgaagagaa attttcaatc gatataagga agctttttct ggaattgata
     4141 gaattgaaat gatgccagaa gtggatggca tggtgccaaa tcgttggttg tctactttaa
     4201 cgatcaatga ttataattct attgatcaag tagttaacgt aataaataaa ctaaatgaaa
     4261 aaaacattga aacaagagct ttgtggaaac cactacattt gcaaccagta tttcaagggg
     4321 taaaaatgta ttaccatgat aataatctta gtgtgtcaga aaagttattt aatagcggct
     4381 tatgcttacc ttctggaagt aatatgacag atagtgaaca agaatttgtt attgatgagt
     4441 ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat aggaattatt ggtttaggtt
     4501 ccatggggaa aagaagactt agactcattc aagaacactt taatgatatt ttactatgtg
     4561 gtgttgatac taatgag
//
LOCUS       DACDKJ010000122         4468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDKJ010000122 DACDKJ010000000
VERSION     DACDKJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.128"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            316..1047
                     /locus_tag="IV328_002850"
     CDS             316..1047
                     /locus_tag="IV328_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8938910.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1192..3219
                     /locus_tag="IV328_002851"
     CDS             1192..3219
                     /locus_tag="IV328_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8938911.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3327..4424
                     /locus_tag="IV328_002852"
     CDS             3327..4424
                     /locus_tag="IV328_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8938912.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1568 a          717 c          923 g         1260 t
ORIGIN      
        1 ataatataca ttttttattt aattataatt aatattgatt gaagtctagg cagtcaagtc
       61 acattttgat agataggagg gggacttacg aattatttta aaggctatga cagctgtatt
      121 tctagattgt gtttgcatca agttgcaata aaaactagaa atcaaatcgg gctgataatc
      181 atttataact aatcgataca taggaccaaa tataggtcta tgttctttta tttcataagt
      241 atcgtaaaac agaattttgc tatggattaa tccaatcgac tgatatgttt agtaaataaa
      301 gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa cagcagcaat gatgttattc
      361 agcatggtag caagtacgac ttttgtttct gcaactgaag tatacccaaa ggaatataac
      421 acagaaggaa cgattacatt tgaagcagga gatgagggtg taactcctcc tgttgatcca
      481 gagaatcctg atcccaataa acctgtcgat ccatcagatc ctccaagccc tggaacagga
      541 ggggcattat cgattgatta tggatcaaaa ttcaaatttg ggactcaaaa aatttctact
      601 gcggataaaa cgtattatgc agctgctgat gtgatgaacg atgggtctag aaaaccgaca
      661 tatgtccaag tgacagatag gagaagtacc ttatctgggt ggaaattaag tgtctctcaa
      721 ccagaacagt ttaaaactgc cagtggggat gaactagttg gagcacagct gaaatttacg
      781 aaagggcagg ctgtttcttt ggttgatcca acatatacgc cgcaaacggt gaattctgaa
      841 ttaacgttga caccgggagg aaacaatacg ttagcaatta atgctaaatc aggaacaggt
      901 gtggggactt gggtctatcg atttggtgca aatgaaaatg aaaatcaaga tgcggtacag
      961 ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat attccactaa attagtttgg
     1021 acattagaag atacacctaa caactagttt actgaaaaga actgttaaaa ataaatacgt
     1081 agtcatattg tcttgcgagg taatcgagca aaaattgtca aaggctaggc taaatttgtt
     1141 tggatacgaa tagttcatct aggaatttaa cgatcaggag gaaaagatat attgaaaaag
     1201 aggaggatcg tgctcatttt aagcagtctc ttagtgacta atatctttcc gatcataggg
     1261 tactctttgg aattgaatca gttggagcat ggaaatagaa gagacgaaga aagcaaactc
     1321 acttcgacat tcgacagttt aatagaatcc aaccagacac aagaaagcag tgaggattta
     1381 agtatttcga gtagtggaga gaccactgac aacgtagaag ctgagggttc ggacaaacaa
     1441 ggactagaag atgcaccaat ttctgcggtt gagctgcctg acgatcccaa tattatttct
     1501 atcgataaaa tttttcaaac acctatcggt actagcacgt ccatattgga aaatgggaag
     1561 ttactgcagt tgaacccagc agaaaggtct caaagaggag ctatttggtc aaataagaag
     1621 attagtttac tctcggattt tacatttaaa tcttatttat acttaggaaa tcaacgagat
     1681 agagcgggag atggcatgac ctttacattg actggcgatc ctagaatggc tagtaatccc
     1741 agtcaagtta ttggatcgcc aggtatggga attggctcat attctactcg ttctggccaa
     1801 ccttacattg cgaatgcgct ctcgattgag tttgatacgt ataaaaatag tggttcttct
     1861 gatcgaatgg atcgagagat aactgctgat aatagagggc atggacatgt cgcttttgtc
     1921 acgcctaaac caaataataa taattattct ggagagcata ctggcgtgac ggtagctcct
     1981 acatttcttt ctaatggcac atggcgcttg ttaataatta aatgggacgc ggagcaaaga
     2041 agtcttactt atgatttgga aggtgtcggt cactcttctc ggattatacc tgatctcatt
     2101 aggcagtttg gttctacaga agtaaattgg gggtttactt cttcaacagg tacttttttt
     2161 caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg tcacatcaac agctgatgta
     2221 aaagtcaacg atagtgatta tggggccatc gctgaagctg gaggaaacga taagataact
     2281 atcagaaata cattgaaggt cgagggggat tttcttgaat ataagaaaat gcagactagt
     2341 atcgacctcc cacaagaatt aactattccc gagcatatct tgatcaatgg aaatcaaatt
     2401 gatgccaaag atatcttgat aaccggaaat cgagtcacgc taaatctgac agattatttt
     2461 attgctggag atacgttaat aatcgaattg gagacaactt tgcaggaagt aattcctgaa
     2521 aaagtactca ctatgcattt tgaatacctt gaaaacggga atttgattga gcaatcgaat
     2581 gaaataaacc taaagatcgc aaagctgaag gagatagata tccatgttta ttataaagat
     2641 aaagacagtc agattgatat tgctgaaatg aaaacaatca ctgggaaaat aggagagaag
     2701 tataaagaaa ctcctattga tattgaaggg tatgtttttg actcggattc agcgaataca
     2761 gaaggcatct tctccgaaaa tacagaggat atctattttt attacagaaa aggcaaactt
     2821 tatctctcaa ctgctcctca atatgtcaat tttggaaaac ataaaatatc cagtagacca
     2881 ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta tagatgaacg ggcttttgga
     2941 agttggaaat tacaattgaa acaagagaaa gttcttacaa atggagattt tgagcttcca
     3001 gaagctcttt cctttgttac ctctgaaaat acaacggtta tagggacatc cgctgttacg
     3061 attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct ctcttttaga ttctacagct
     3121 catcgtggaa tcagaataga tattcctgta gaaaaccaaa gaatcggaac ctttgagggg
     3181 aaactatctt ggatacttgc agatgtccca ggaaattaac aggaagagaa gaatataaca
     3241 aaattattga gtttcaagaa gggcacaggt accataggct cttgtgtccc acagagacaa
     3301 gattttgtta tagtcttctc ttacatatga ttaaaggagc gattttcttg agaccaaaac
     3361 aacaattatt tataacactc ctagttattt taggggcact ttttaacaat atatcagtgt
     3421 cagctagtgc atttaatttt tcagtgacgc cgataacttc agagaatcaa atcgacaaga
     3481 gaaaaactta cttcgatctt caacttgatc cggatcagga agtagaagta aaagcggaat
     3541 tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc tgtgaatagc gcaaccacca
     3601 attcaaatgt gatggttgaa tacggaaaaa acgagattga aaaagacatg agcctgatct
     3661 ttgatctggt agattatgtg agctatccac agatagtgac gctaaaacct aaaagtgtcc
     3721 aaaccgttgc ttttcatgtc aggatgccga atgagcgatt tgatggtgta ttggctggtg
     3781 ggatcacatt ccaagaaatc cagacggaaa aggaccaaac agaaactaag gatcaaagct
     3841 tatcgattga gaatgaatac acctatatta ttgctttgtt gatgcaacag acactgaacg
     3901 aagttgctcc gaaccttact cttcatgaag ttaaaccaga tcaaatcaat gcaagaaatg
     3961 tcatcttagc aaacgttcaa aatgatcaaa aaacctatat caatcaagta gttattgaaa
     4021 caaaaattac aaaaaaagga cattctgaag ttttatatca agaggaaaaa gaaggattgc
     4081 agattgcgcc gaatacaaat ttttcttttc caactgcttt aaatggtcaa ccgctaaccc
     4141 caggtgaata tcaccttaca atgactgttt taggaaatga aaatgaaaat ggaaaatttt
     4201 cgagaaaaaa aggaaatact acgatcaact ataccaatca atgggtattt gaaaaagatt
     4261 ttacaataga cggaaaagta gccaaggaat tgaacacgac ggatgttacg attaaaaaag
     4321 acaataattg gatttatatc ttagtagctt cacttttctt attgtttatt ttattatgca
     4381 tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa gtaaggttaa gataacaaat
     4441 agtaaatttt tattattgta ggatggtt
//
LOCUS       DACDKJ010000123         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDKJ010000123 DACDKJ010000000
VERSION     DACDKJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.129"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IV328_002853"
     CDS             complement(543..2048)
                     /locus_tag="IV328_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8938913.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IV328_002854"
     CDS             complement(2045..2731)
                     /locus_tag="IV328_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8938914.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV328_002855"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV328_002855"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8938915.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACDKJ010000124         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDKJ010000124 DACDKJ010000000
VERSION     DACDKJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.130"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IV328_002856"
     CDS             690..1658
                     /locus_tag="IV328_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8938916.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IV328_002857"
     CDS             1669..2484
                     /locus_tag="IV328_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP8938917.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IV328_002858"
     CDS             2552..3262
                     /locus_tag="IV328_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8938918.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IV328_002859"
     CDS             3298..>4425
                     /locus_tag="IV328_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8938919.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACDKJ010000125         4347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDKJ010000125 DACDKJ010000000
VERSION     DACDKJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.131"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(387..1853)
                     /locus_tag="IV328_002860"
     CDS             complement(387..1853)
                     /locus_tag="IV328_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8938920.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IV328_002861"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IV328_002861"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8938921.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1414 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaatt
//
LOCUS       DACDKJ010000126         4313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDKJ010000126 DACDKJ010000000
VERSION     DACDKJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.132"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IV328_002862"
     CDS             complement(<1..281)
                     /locus_tag="IV328_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8938922.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..1272)
                     /locus_tag="IV328_002863"
     CDS             complement(730..1272)
                     /locus_tag="IV328_002863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938923.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            complement(2049..2239)
                     /locus_tag="IV328_002864"
                     /pseudo
     CDS             complement(2049..2239)
                     /locus_tag="IV328_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2359..2721)
                     /locus_tag="IV328_002865"
     CDS             complement(2359..2721)
                     /locus_tag="IV328_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938924.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="IV328_002866"
     CDS             complement(2725..3033)
                     /locus_tag="IV328_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938925.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="IV328_002867"
     CDS             complement(3042..3461)
                     /locus_tag="IV328_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938926.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="IV328_002868"
     CDS             complement(3454..3687)
                     /locus_tag="IV328_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938927.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4313)
                     /locus_tag="IV328_002869"
     CDS             complement(3684..>4313)
                     /locus_tag="IV328_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8938928.1"
                     /translation="ISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRV
                     VDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLEL
                     QLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKK
                     KYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1221 a          959 c          618 g         1515 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aat
//
LOCUS       DACDKJ010000127         4310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDKJ010000127 DACDKJ010000000
VERSION     DACDKJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.133"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IV328_002870"
     CDS             complement(65..1285)
                     /locus_tag="IV328_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8938929.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IV328_002871"
     CDS             complement(1305..1910)
                     /locus_tag="IV328_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8938930.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IV328_002872"
     CDS             complement(1959..2891)
                     /locus_tag="IV328_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8938931.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="IV328_002873"
     CDS             complement(3312..4172)
                     /locus_tag="IV328_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8938932.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DACDKJ010000128         4192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDKJ010000128 DACDKJ010000000
VERSION     DACDKJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.134"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            27..362
                     /locus_tag="IV328_002874"
     CDS             27..362
                     /locus_tag="IV328_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938933.1"
                     /translation="MKQYLDQWNIIEGALTVERINEIPDCLEKDHLFLIRKMLKEENF
                     EPNDFIVVEYPATGIYCCNHINNGQYFIIQEEKNKLVPYYTTCDMNEEGINNFPCTSI
                     KESMKLIEM"
     gene            383..652
                     /locus_tag="IV328_002875"
     CDS             383..652
                     /locus_tag="IV328_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938934.1"
                     /translation="MQTFTQHELEIISGHKQIAGSNANKLNVIKSEVGEMLSMLKSDL
                     GKQYIEDDEQRHYTLKNYQSVYDKLEKMSEDEFKQIDLSGIEELE"
     gene            887..1300
                     /locus_tag="IV328_002876"
     CDS             887..1300
                     /locus_tag="IV328_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8938935.1"
                     /translation="MEQKLAKTPENILQIAKTYDNDERNIQKKFRVSEREKFLIDYKM
                     SQANTRNFSRYARIMCLSGLMITTDFSVLKNLTTELGRIGNNINQLAHRANISNSVSK
                     EDFLELKINYDQLTKLINEELLFRMRRARDNQRFI"
     gene            1300..3594
                     /locus_tag="IV328_002877"
     CDS             1300..3594
                     /locus_tag="IV328_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8938936.1"
                     /translation="MAVTKIHQIKTTLNKAIGYITNTEKTNNGMLVSSYNCQSQFAEV
                     EMQMTREYAREVKGDYRKVGGSEVLAHHLIQSFSPEDKVTPELAHELGRQLIDELTEG
                     KFEYVIATHIDQEHIHNHIIFNSVSFYDLKKFRSQPYRTANKIKEISNRICAEHDLSL
                     SPKKEKLKDSYKSYSNRRNNTSFRSEIRKRLTMCLNESTSWEEFEEKADHLGVNIDRN
                     GKHISYALLSEGQQRRTRGDKLDDLETFTESNISQKLSANQTYTNQLKEAIEETFFSS
                     DNLEDFSFLLEQEHGITFKLNKQDQFVFHFNDVNDFKWNESLLPDSYQIKNLKDYFRE
                     SYEFTNDSSLNSIEERYREKEKSVIVQEDTPIQLQEFQIEKATKEGLLVSIEHEDLNG
                     LVFVPNNYVDVNEETGEYTIWINDKFDYTLTDKQTNEQTSFTVKGEGIIRGLEKMQGI
                     QPQEIKLSSKNILSMSNRGVSISIPELNIKRLFIPSEYVVIDEISKSCSILIGEKWNY
                     YAEPLPENDDNSNKKLPYQKFTGRSLISSLKETPELLDVFLKPKFDQLHYQSRKRYTN
                     QLVEALNTIREEKIVSASQLEQRLNKIVEQKNQAKEKIAEIDGKIKNYNNVAKLLVTY
                     EKYLPIMNEIERATMLQKIKLEQKYKEEVRQFKFAEKKLLASDNLRPDLTKEKIISVA
                     KNQEKQRKRLIEGYKHYENKLNRLIDVQEILQELDRNGLFDLSGYRLINREEKLTDKI
                     EKYEKEVEETQQKNSKEKSKEREI"
     gene            3733..3945
                     /locus_tag="IV328_002878"
     CDS             3733..3945
                     /locus_tag="IV328_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938937.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGSV
                     AVVITAVAKASDSVLAWLKFKKEQKK"
BASE COUNT         1674 a          556 c          738 g         1224 t
ORIGIN      
        1 ttttataata aatgagggat ataaaaatga aacaatattt agatcaatgg aatataattg
       61 aaggagcatt aactgtagaa agaattaatg aaatacctga ttgtctagaa aaagatcatt
      121 tatttttaat tagaaaaatg ttaaaagaag agaattttga acccaatgat tttattgttg
      181 ttgaatatcc agctactgga atatactgct gtaatcatat taacaacggt caatatttta
      241 ttattcaaga agaaaaaaat aaattagttc catactatac tacttgtgat atgaatgaag
      301 agggaattaa taattttcct tgtacgagta tcaaagaatc catgaaatta atagaaatgt
      361 aaaagagagg gacatataaa tcatgcaaac atttactcaa cacgaattag aaattattag
      421 cggacacaaa cagattgcag gttcaaatgc aaataaatta aacgtcataa aaagcgaagt
      481 tggagaaatg ctttcaatgt taaaaagtga tctaggaaaa caatatattg aagatgatga
      541 acaacgacat tacacattaa agaattatca gtctgtttat gataaacttg aaaaaatgag
      601 tgaagatgaa tttaaacaaa ttgatctgag cggtattgaa gaattggaat agaaacgggg
      661 attggggcag cgccccaaaa tgcaaaaaga agaagtcgcg tgagcggctt tttctttttt
      721 tccgcaagtt ggtaccaact tgcaaatctt gctgtaagac cgcccccctg aaatcttcga
      781 tttctaatcg gagggcggtc acacagtgga aattttcgtt tttaaacaaa gataaaacac
      841 taaactagtg aaaaaaaaca aaaaaaattg gtaaggaggg gaaaatatgg agcaaaaact
      901 agctaaaact cctgaaaata ttctacaaat cgctaaaact tacgacaacg atgaaagaaa
      961 tattcaaaaa aaatttagag tatctgaaag agaaaaattc ttaatagact acaaaatgag
     1021 tcaagcaaat acaagaaatt tttcccgata cgctcgtatt atgtgtttaa gtggtttaat
     1081 gattacaaca gatttttctg ttttaaaaaa tttaactaca gaattaggac gaattggaaa
     1141 taatatcaat caattagctc acagagctaa tatttcaaac agtgtttcaa aagaagactt
     1201 tttagaatta aaaataaatt atgatcaatt gactaaatta attaacgaag aattactttt
     1261 tagaatgaga cgtgctagag ataatcagag gtttatttaa tggcagtgac aaaaatacac
     1321 caaataaaaa ctactttaaa taaagcaatt ggctatatta caaatacaga aaaaacaaat
     1381 aatggaatgc tggttagtag ttataattgt caaagtcaat ttgcagaagt tgaaatgcaa
     1441 atgactaggg aatatgcacg tgaagtaaaa ggcgattatc gaaaagttgg tgggagtgag
     1501 gttttagcgc accacttaat ccaaagtttt tcgcctgaag acaaagtgac accagagctt
     1561 gctcatgaat taggacgaca attgattgat gaattgactg aaggaaagtt tgaatatgtg
     1621 attgccactc acatagatca agaacatatt cataatcaca ttatttttaa cagtgtttct
     1681 ttttatgatc tgaaaaaatt cagaagtcaa ccttatcgaa cagcaaataa aattaaagaa
     1741 attagtaatc gtatttgtgc tgaacatgat ttatctttat caccgaaaaa agaaaaatta
     1801 aaagattcct acaaatctta ttctaacaga agaaataaca cgagctttcg ttctgaaatt
     1861 cgtaaacgat tgaccatgtg cttaaatgaa tcaacttcat gggaagaatt tgaagaaaaa
     1921 gcggatcatt taggggtaaa tattgatcga aatgggaaac atatttctta tgctttattg
     1981 tccgaaggac aacaaagacg gactagaggg gataagttag atgacttaga aacatttaca
     2041 gaaagtaata tttctcaaaa actttctgct aatcaaacct acactaatca gttgaaagaa
     2101 gcgatcgaag agacattctt ttctagtgac aacttggaag attttagttt tcttttggaa
     2161 caagagcatg gaattacctt taaattaaat aaacaagatc aatttgtttt ccatttcaat
     2221 gatgtaaatg attttaaatg gaatgaaagc ttgctgccag atagttatca gattaaaaat
     2281 ctaaaagatt attttagaga atcctatgag tttacgaatg actcttcctt aaattcaatt
     2341 gaagaaagat atcgtgaaaa agaaaaaagt gtgattgttc aagaagatac gcctattcaa
     2401 ttgcaagaat ttcagataga gaaagcgacc aaagaaggat tgctagtgtc tatcgaacat
     2461 gaagacttaa atggtttagt gtttgtccca aataattatg ttgatgtaaa tgaagaaact
     2521 ggagaatata caatttggat caatgacaaa tttgattata cgcttactga caaacagaca
     2581 aacgaacaaa ctagttttac tgtaaaaggg gaaggaatca ttcgtggact ggaaaaaatg
     2641 caagggattc aaccacaaga gataaaactt tctagcaaaa atattttatc tatgagtaat
     2701 cgtggtgtat ctatttcgat accagaactg aatatcaagc ggctctttat tcctagtgag
     2761 tatgttgtaa tcgatgaaat tagtaaatct tgttctatct taattggaga aaagtggaat
     2821 tactatgcag aaccactccc tgagaacgat gataattcaa ataaaaaatt accttatcaa
     2881 aaatttactg gacgttcttt aatcagtagt ttaaaagaaa ctcctgaatt acttgatgtg
     2941 tttctaaaac ctaaatttga tcagctacat tatcaatcaa gaaaacgata tactaatcaa
     3001 ttagtcgaag ctttaaatac gattcgtgaa gaaaaaattg tttcagcttc acaattagag
     3061 cagagattga ataaaattgt tgagcaaaaa aaccaagcaa aagagaaaat agctgagatt
     3121 gatggaaaaa taaaaaatta taacaatgtc gcaaaacttt tagtaactta cgaaaaatat
     3181 ttaccgatca tgaatgaaat tgagcgtgct acgatgcttc aaaaaattaa attagaacaa
     3241 aagtacaaag aagaagtacg ccaatttaaa tttgctgaaa agaaattact agcttcagat
     3301 aatctacgtc ctgatttaac gaaagaaaag ataatatctg tggcgaaaaa tcaagagaaa
     3361 cagaggaaac gacttataga gggctataag cattatgaaa ataaattaaa tagattgatc
     3421 gatgttcaag aaatattgca agaactagat agaaatggat tatttgatct tagtggttat
     3481 cgtttgatta atagagaaga aaagctaacg gacaagatcg agaagtatga aaaagaagta
     3541 gaagaaactc aacaaaaaaa ttctaaagag aagagtaagg aacgtgaaat ataaataatt
     3601 gaaaacacta tatgttccga aaagtgaata tatctcttca taaaataatc aacatattta
     3661 aaaaattcaa tcagcctttt ttgtcgagat aaagttcttc attctatgat ataatgttgc
     3721 tgtagaaagt ttgtggcggt ttggcaaatc caattgaagg agcgtggtgc ctatgggctt
     3781 attagcttat cggacgtact ctgtagaaag gaaatgcctg atttgttctc attggatcaa
     3841 atcattgcta ttattggatc agtagcagta gttattactg ctgtagcgaa agctagcgat
     3901 tcggttttgg catggttaaa gttcaaaaaa gaacaaaaaa aatagagccg tctcaacttt
     3961 tggcgaagta gacgggctct aaacctgatc aaaaatgcca tccgctttga acggactaca
     4021 ctgggcatgt tacagcatgc cctttcattt ttattataac tcattaatct aaatttgaaa
     4081 agtaactttg tttgctaaag ggtgttctac atgacaataa acatagcata tatattttat
     4141 ttttatatta tcaactttga aaaaatttat tctcttctag aaatagtatg tg
//
LOCUS       DACDKJ010000129         4120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDKJ010000129 DACDKJ010000000
VERSION     DACDKJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.135"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            573..>4120
                     /gene="esp"
                     /locus_tag="IV328_002879"
     CDS             573..>4120
                     /gene="esp"
                     /locus_tag="IV328_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8938938.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDIIENPGDNIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVL
                     KAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVTFTAGEG
                     TSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATK
                     LDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKK
                     GTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         1541 a          646 c          846 g         1087 t
ORIGIN      
        1 ttataaagaa aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg
       61 ctaaaaacga gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca
      121 ttcagaaatg ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga
      181 agaaggaatt tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata
      241 aggctcctta ttccgttatt tgaatactct gaagtttaat ctaagaaagg gtaatatttt
      301 gaaaacatgc ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt
      361 tttatatgtt ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag
      421 aaatgtactt tttttgttgt aatgttcatg aaattctcta agtaaattag ttaacataag
      481 gttagtaaga ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactag
      541 gaaagtaatt attaagggag ttgatttgaa taatggttag caagaataat aagagagtat
      601 ttctagaaaa aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg
      661 ttgcatctgt tttgattggt gttggtcttg tcttgggaac aactgaacta gttaaagcac
      721 aagatgaaat ttctccaagc agcccacttg aaacagcgat aagctccgta caagtaggtg
      781 acaaagtagc ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact
      841 tttcggattt acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa
      901 ttggatttga agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc
      961 gtcttcaatt agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga
     1021 aaggaattgg atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta
     1081 caaatatttg gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggcagaaa
     1141 caaccgatac gcaaaccgct cctaatgggg taattacgtt tgaaaagagc ctagatgaaa
     1201 tttttaaaga gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact
     1261 tggtaagtca tcaagacgat gataaaattg taccgggtat tgatagtacg ggctactttt
     1321 taacagattc agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt
     1381 ttaaacatgg atcagtaagt gcaaaatatg agaaacctaa tactcaaaca aaagatggtt
     1441 caggatccac aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata
     1501 atttctcata ttcatcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg
     1561 aacgattagt tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa
     1621 catatgatgt ttcgtttgaa tccggaacaa aagtagctga tttagcaatc gagcgacggg
     1681 aaggtcatga aaattatggc ataggttcaa taactaataa tgatttaact aaacttatcg
     1741 attttgctaa tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc
     1801 cattagatga aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat
     1861 ttggtgaaga tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga
     1921 acacttatgg aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg
     1981 ataaagaaga gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat
     2041 atgatgttga cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc
     2101 acaaagccca acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa
     2161 atgaaaaaga tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt
     2221 caagtaaatt aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata
     2281 aaccagaaga aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg
     2341 tggtagttgg atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg
     2401 aagatgcaga gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga
     2461 aaggtattgt aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg
     2521 taaaagcttc tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag
     2581 aaacgacagt tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag
     2641 atgccttttc aatttcagat gtctataata agtacttcgg taatgcaagt gttgatgcaa
     2701 acactggtat tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg
     2761 gaacaattcc tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag
     2821 taactgtaag taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca
     2881 aagtaacctt caccgcaggg gaaggaacaa gtattgaaag tggaacaaca gtctttgcag
     2941 tgaaagacgg cgtaagcttg ccagaagata aacttccagt gttgaaagca aaagatggtt
     3001 atacagatgc gaaatggcca gaagaagcaa ctcaaccaat tacagcagat gatacagaat
     3061 ttgtatcaag tgcaacaaaa ctggatgata tcattgaaaa tccaggagaa aacattcctg
     3121 caggctacca caaagtaacc ttcaccgcag gagaaggaac aagtattgaa agtggaacaa
     3181 cagtctttgc agtgaaagac ggcgtaagct tgccagaaga taaacttcca gtgttgaaag
     3241 caaaagatgg ttatacagat gcgaaatggc cagaagaagc aactcaacca attacagcag
     3301 atgatacaga atttgtatca agtgcaacaa aattggatga tatcattgaa aacccagggg
     3361 ataacattcc ggctggctat cataaagtaa cctttactgc aggagaagga acaagtattg
     3421 aaagtggaac aacagtcttt gcagtgaaag acggcgtaag cttaccagaa gataaacttc
     3481 cagtgttgaa agcaaaagat ggttatacag atgcgaaatg gccagaagaa gcaactcaac
     3541 caattacagc agatgataca gaatttgtat caagtgcaac aaaactggat gatatcattg
     3601 aaaatccagg agaaaacatt cctgcaggct accacaaagt aaccttcacc gcaggagaag
     3661 gaacaagtat tgaaagtgga acaacagtct ttgcagtgaa agacggcgta agcttaccag
     3721 aagataaact tccagtgttg aaagcaaaag atggttatac agatgcgaaa tggccagagg
     3781 aagcaactca accaattaca gcagatgata cagaatttgt atcaagtgca acaaaactgg
     3841 atgataaatc tgacgctgac aaatataatc ctgaaggtca aaaagtgact acagaattga
     3901 ataaggaacc agacgcattt gagggaatta agaacaaaga agatttacca aaagatacta
     3961 agtatacttg gaaagaaaaa gtagatgtta gcgcagctgg aaataaaaaa gggactgttg
     4021 tagtgacata tccagatgga tcatctgatg aagttgaagt agatgttaca gtgacagaca
     4081 atcgttctga cgccgataaa tatgagccaa cagtagaagg
//
LOCUS       DACDKJ010000130         4084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDKJ010000130 DACDKJ010000000
VERSION     DACDKJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.136"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..383
                     /locus_tag="IV328_002880"
     CDS             6..383
                     /locus_tag="IV328_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938939.1"
                     /translation="MLIAKRILASIVAIPCFLLSIIVIAVNIISIPILLVIQILKKFV
                     ILFITLLWIGFIGLFIYGLSSLGFSETISMFTVPTIGILSLTVILSIFTFISDKIPDF
                     LGIVISFFLSIPKFLWGTEETEE"
     gene            404..1471
                     /locus_tag="IV328_002881"
     CDS             404..1471
                     /locus_tag="IV328_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8938940.1"
                     /translation="MFSKKRYVQFIEQRDIELPETPVEIYRGIYLLEQENDRLTDKHT
                     KGSTVILLTDKKGNELNRRLIQLPIEQGIETILTNLLIEPTSNKKQRSSKSQMIVNDI
                     EEPKIKNKKKTVDYQTIFFLFTTNLAKKIYMALIIVLFLFFTIFFVLNNHSSTSTDSQ
                     SYHELLKKKDYEKIIKQYPEKENELVEKLYSEKNSKELKELAEKSDSQIALFYWDFLN
                     EHWKAVTTMKNVTPNETIQAMRGYAYLKQGKLDEAELINKEIDNQTLKDQIYQFKKQE
                     AYKALHEKDTEKAEKINKEINDSELSEDIKVAKSMVNLLQKYEKDRSDSKLSEDERKE
                     AENNYKMWSENLKQLGGNDNA"
     gene            1464..3044
                     /locus_tag="IV328_002882"
     CDS             1464..3044
                     /locus_tag="IV328_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938941.1"
                     /translation="MHNKKKFLVVGLFSAVLLGGAVTYGMYSQQPHNETEKTEQTTKS
                     ESSKHKSKKSAWEESVKGKEDKKDKNKHSTKKADDILAVIADTPLVKQQPNQNKNNLI
                     DRLAVALDDQTKKEADRQEKLIIKDKKSVDEPVLVDLSYKPQLEDTKPSNKENGITEN
                     NKDENSESDKGNDTKPDNSLPDPLPVPEPNPLPDPTPSPDPNPIPTPDPDPDPEPEPT
                     PDPEPSIDEQINQSKEELNDSRKQADELNKQLTQEKDSLDNLQSIGASTEKNVDKAAL
                     QWQKVADLVNEYNQLTNEIKKLIEEDGTVLPINYDLYKETYEKLNQKVTEVKTAQKEA
                     TNTTNEINKNVEQATDTLNNLEETKKEYQNKQKNVSEVDSNVNSAINHAQSNEQVASA
                     VQDEINQAAQSNEQLNETNKTVGEQLNQVDPIESQTDIASAKEQTADINNQAEEQNNN
                     VDDMMNDFNNLPKPSDSEEPSVPENTDQNQDSTSQPQNIDNASVSQNKIENEQVSEKT
                     LPVTNQNSNQSEIEDIQK"
     gene            3063..>4084
                     /locus_tag="IV328_002883"
     CDS             3063..>4084
                     /locus_tag="IV328_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938942.1"
                     /translation="MSYKDSEAKKNNKTAELLSQAEEGIKEVLSSDKYKNFLRVMSKF
                     HNYSLNNTLLILMQNPNATHVAGYKTWKNSFNRQVNKGEKSIKIIGGRPYKRKVEEIK
                     NGQKEEKEVEGISFFPVSVFDVSQTSGEPLPQLMNELSGEVKNYPELYVALKQSTNFS
                     IEFADIQGGTKGYCSPLEKKIVLNNGMSQAQTIKTLIHEITHADLHAPEFDKNSSIKT
                     TKSTKEVEAESTAFVVCEHYGIDTKDYSFPYLAAWSSDKELKELKNSFEVILKQADNL
                     IQKIDKNLAELQKDVTKEKEEKQSTLSLSDQLEEFDDKAKSLNEQREKEKLINKIRQS
                     KEQKDVS"
BASE COUNT         1746 a          595 c          659 g         1084 t
ORIGIN      
        1 aaagtatgtt aatagctaaa agaatattag caagtatagt agcaattcct tgctttttac
       61 tttccataat tgttattgcc gttaacataa tttccatacc tattttgctt gttatacaga
      121 ttcttaaaaa gtttgtaatc ctttttatta cattattatg gattggcttt ataggtctgt
      181 ttatttatgg attaagttct ttaggcttct ctgaaacgat tagtatgttt acagtgccta
      241 ctataggtat actaagtctt acggtaatac tatctatttt tacttttatc tcagataaaa
      301 ttcctgattt tcttggaatt gttatttcat ttttcctttc tatcccaaaa tttctatggg
      361 gaactgaaga aactgaagaa taaaacattc aagggggaaa aatatgtttt caaagaaaag
      421 atatgttcag tttatagaac aaagagatat tgaattacca gaaacccctg tagaaattta
      481 caggggtatt tatttacttg aacaggaaaa cgatcggcta acagataagc atactaaagg
      541 ttctacagta atattattaa ccgacaaaaa gggaaatgaa ctaaatagac gactaatcca
      601 attaccaata gaacaaggta tagagactat tttgactaac cttttaattg aacctacctc
      661 aaataaaaaa caacgttctt ctaagtcgca gatgatagtg aatgatatag aagaaccaaa
      721 aataaagaat aaaaagaaga ccgtagatta tcagacgatc ttctttttat ttactactaa
      781 tttagcaaaa aaaatctaca tggctttgat aatagttctg ttcctatttt tcacaatttt
      841 ttttgtatta aataatcatt catcaacttc tactgattct caaagctatc atgagctgtt
      901 aaagaagaaa gattatgaaa aaataataaa acaatatcct gaaaaagaaa acgaattagt
      961 agaaaaactg tatagcgaaa aaaattctaa ggaattaaaa gaattggcag agaaatctga
     1021 ttcacagata gctctttttt attgggattt cttgaatgag cattggaaag cagtaactac
     1081 gatgaaaaat gttacaccaa acgaaacaat acaagctatg cgtggttatg catacttaaa
     1141 acaaggaaaa ctagacgaag cagaactaat taacaaagaa attgataatc aaacattaaa
     1201 agatcaaatt tatcaattta aaaagcaaga agcatataaa gctctgcatg aaaaagatac
     1261 cgagaaggca gagaaaataa ataaagaaat aaatgattct gaactatccg aagatattaa
     1321 agtagctaaa agtatggtta atctacttca aaaatatgaa aaggacagat cggattcaaa
     1381 attatcagaa gatgaaagaa aagaagctga gaacaattac aaaatgtggt cagaaaactt
     1441 aaaacaacta ggaggaaacg ataatgcata acaaaaagaa atttttagta gtgggactat
     1501 tttcagctgt tttattaggg ggtgcagtta cttatggcat gtattctcaa caaccacaca
     1561 atgagactga aaagacagaa caaacaacta aatcagagtc cagcaaacac aaaagtaaga
     1621 aatcagcatg ggaagaaagt gtgaaaggca aagaagacaa aaaggacaaa aacaaacatt
     1681 ctacaaagaa agctgatgat atattagctg ttattgctga tacaccgttg gttaaacaac
     1741 aacctaatca aaacaaaaat aatctaattg atagattagc agtagcttta gatgatcaga
     1801 ctaaaaaaga ggcagacaga caagaaaaat taattattaa agataaaaaa tctgtagatg
     1861 agccagtact agttgatttg agttataaac ctcaacttga ggatactaaa ccttcaaata
     1921 aagagaacgg tataacagaa aataataaag atgaaaatag cgaatcagat aaaggaaatg
     1981 atactaaacc tgataattca ttacctgatc cgttgcctgt accagagcca aatccattac
     2041 ctgatccaac acctagtcca gacccaaatc caataccaac accagatcca gaccctgatc
     2101 cagagcctga gccaacacca gatccagaac cttcaattga tgaacaaatt aatcaatcta
     2161 aggaagaact caatgattct aggaaacagg ctgatgaatt aaataaacaa ctaactcaag
     2221 aaaaagatag cttggataac ttacaaagta ttggagcaag tactgaaaag aacgtggata
     2281 aagcagcatt acaatggcaa aaagttgctg acttagtcaa tgaatataat caacttacaa
     2341 atgagatcaa gaaattaatt gaagaagacg gaacagtgtt gccaatcaat tatgatcttt
     2401 ataaagaaac gtatgaaaaa ctaaaccaaa aagtcactga agtaaaaact gcacaaaaag
     2461 aagcaactaa tacgacaaac gaaataaata aaaatgtaga acaagcaact gatacgctaa
     2521 ataatttaga agaaactaaa aaagaatatc aaaataaaca gaaaaatgtt tctgaggtgg
     2581 attctaacgt caattcggct attaaccatg cacaatccaa tgaacaagtt gcttcagctg
     2641 ttcaagatga gataaatcaa gctgcacaat ctaatgaaca actaaatgaa actaataaga
     2701 ctgttggaga acagttgaat caagttgatc caattgaaag ccaaactgat attgctagcg
     2761 ctaaggagca aactgctgat atcaataatc aagctgaaga acaaaataat aatgttgacg
     2821 atatgatgaa tgattttaac aatttgccaa aaccgagtga tagtgaagaa cctagcgttc
     2881 cagaaaatac agatcaaaat caagattcaa caagccaacc acaaaacata gataatgcta
     2941 gtgtttctca aaacaaaata gaaaatgaac aggttagcga aaaaacatta ccagttacta
     3001 atcaaaatag taaccaatcc gaaattgaag atattcaaaa ataaaagtga ggaatgaaat
     3061 aaatgtctta taaagatagt gaagcaaaaa aaaataataa aaccgctgaa ttactttcac
     3121 aggcagaaga aggtattaaa gaagtattat cttcagacaa atacaaaaat tttcttcgtg
     3181 ttatgtctaa gtttcataat tacagcttaa ataataccct tttaattctt atgcaaaatc
     3241 ctaatgctac tcatgttgct ggttacaaaa catggaaaaa ctcttttaat agacaagtaa
     3301 ataaaggaga aaaatccatt aaaataattg gcggtcgtcc ctataagagg aaagtagaag
     3361 aaataaagaa tgggcaaaaa gaagaaaaag aagttgaagg tatttcattt ttccctgttt
     3421 cagtttttga tgtttctcaa acttcaggag aacctttacc gcaactaatg aatgaattaa
     3481 gtggagaagt aaaaaattat ccagagcttt atgttgcctt gaagcaaagc accaattttt
     3541 ctatagaatt tgcagatata caaggtggca caaaaggata ttgttctcca ttagaaaaga
     3601 aaattgtgtt aaataatggt atgagtcaag cgcaaacaat aaaaactctg attcatgaga
     3661 tcactcatgc tgatttacat gcaccagaat ttgataaaaa ttcgtcaata aaaactacaa
     3721 aatctacaaa agaagttgaa gctgaaagta cggcttttgt tgtttgtgaa cattatggaa
     3781 tagatacaaa agactatagc tttccatatt tagcagcttg gtctagtgat aaagagttaa
     3841 aggaattgaa aaatagcttt gaagtcatat taaagcaagc agataattta attcaaaaaa
     3901 tagacaagaa tttagcagaa ttacaaaaag atgtgacaaa agaaaaagaa gaaaaacaga
     3961 gtactttgtc attatctgat caattagaag aatttgacga taaagcaaaa tctttaaatg
     4021 aacaacgtga aaaagaaaaa ttaattaata aaattcgcca atcaaaagag caaaaagacg
     4081 tttc
//
LOCUS       DACDKJ010000131         4044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDKJ010000131 DACDKJ010000000
VERSION     DACDKJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.137"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IV328_002884"
     CDS             <1..391
                     /locus_tag="IV328_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP8938943.1"
                     /translation="KKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLT
                     DSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSD
                     WKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            394..1290
                     /locus_tag="IV328_002885"
     CDS             394..1290
                     /locus_tag="IV328_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8938944.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1340..1729
                     /locus_tag="IV328_002886"
     CDS             1340..1729
                     /locus_tag="IV328_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8938945.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1716..>4044
                     /locus_tag="IV328_002887"
     CDS             1716..>4044
                     /locus_tag="IV328_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8938946.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1371 a          639 c          850 g         1184 t
ORIGIN      
        1 aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg atgagtattc
       61 aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta aggaaattcc
      121 caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa aagttacaga
      181 cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc gggctaagat
      241 tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag agctgaataa
      301 atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag gtatcaaaaa
      361 cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga ttctttatat
      421 caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga aataaagctt
      481 aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc atatcaaaat
      541 ctctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac aggaatagag
      601 gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag agcttgtatt
      661 aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct gacaaaaaat
      721 tccattacta atcaagatat atataatgac atacttgata taagagttag tggggaaagt
      781 agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga tgcacaagtt
      841 cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga actaaatgga
      901 aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat tctttctatg
      961 attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga tttggatagt
     1021 caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa cagacaaatt
     1081 ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg gttgattagc
     1141 atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga acccaatatc
     1201 aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa tagagcgaaa
     1261 cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat tattatgtcc
     1321 gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct gggcagttga
     1381 aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt ttaaccaaat
     1441 ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc ctccactatc
     1501 tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca cttggtttat
     1561 gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct ttatcagcta
     1621 tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga agaaacgatg
     1681 ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag tatattgaaa
     1741 ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta gtgccttaca
     1801 attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt cgccaactaa
     1861 ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa agtagtattc
     1921 gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa gtagcgaaac
     1981 aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt cagattgatt
     2041 atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat ctaaagagtc
     2101 tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat catcatttga
     2161 tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag cttgaaaaat
     2221 taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg agtgacctga
     2281 cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa tacgaatttc
     2341 agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac ttattacgtc
     2401 caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat gagaatcatg
     2461 agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa tttccttcct
     2521 cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca agtatgaatg
     2581 tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa aaagaactga
     2641 aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat gtcttagatg
     2701 ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa tctatgtaca
     2761 aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag cgacgctgtg
     2821 atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc tttggcgata
     2881 tgatggggct acatgaagaa ttcttaccct caagtaaacg atatatgaac gactatattc
     2941 agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag atgctaggag
     3001 aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt tacctgaaac
     3061 cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc gccgctttcc
     3121 tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac tatgctgttt
     3181 tgttcggtgg tcaagctgtg attgtcgatc caaaagggga gcgaggaggg tggaaagaaa
     3241 ccttgccaga aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc
     3301 aaggtctgct tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa
     3361 ttgatattct gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt
     3421 tacgtaaagc cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta
     3481 acgaattgcg aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct
     3541 ttaccgatta tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta
     3601 gtttagataa acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa
     3661 cgacttttga agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt
     3721 ccacttttgc cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg
     3781 atgaggcttg gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc
     3841 gtgctggtcg ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat
     3901 tagatgaacg tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc
     3961 atgaaatcaa gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa
     4021 aacgcctgcg agatttggaa aacg
//
LOCUS       DACDKJ010000132         4036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDKJ010000132 DACDKJ010000000
VERSION     DACDKJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.138"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            853..1065
                     /locus_tag="IV328_002888"
     CDS             853..1065
                     /locus_tag="IV328_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938947.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="IV328_002889"
     CDS             complement(1343..3142)
                     /locus_tag="IV328_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8938948.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="IV328_002890"
     CDS             complement(3266..3862)
                     /locus_tag="IV328_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8938949.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DACDKJ010000133         3934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDKJ010000133 DACDKJ010000000
VERSION     DACDKJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.140"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..193)
                     /locus_tag="IV328_002891"
     CDS             complement(<1..193)
                     /locus_tag="IV328_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938950.1"
                     /translation="MIEEKKKVLYSNKPEFKKLVMQYAKKNIGRSITYDTFIKWLDKY
                     GYDLSQYDTCWQAVFKSLLQ"
     gene            complement(204..401)
                     /locus_tag="IV328_002892"
     CDS             complement(204..401)
                     /locus_tag="IV328_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938951.1"
                     /translation="MEWLKNEKELLQFLSDMKRKNQVANLDNYIKWIKDQSVQIKQDR
                     EYGNLNIKIAVTDKQNNILIY"
     gene            complement(425..811)
                     /locus_tag="IV328_002893"
     CDS             complement(425..811)
                     /locus_tag="IV328_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938952.1"
                     /translation="MNRIRFGKSNKNEFSSIFKVNVGIALVFFSLGIWGGMRVQCEQS
                     YFDNTIDEVYFEYDLATHRNYNPKESHEVSLRRVQYLTWDLYPLLKYPESVTSKLFYI
                     DKRNNEKVDKAWVTLLKLQKIALEEK"
     gene            complement(1609..2001)
                     /locus_tag="IV328_002894"
     CDS             complement(1609..2001)
                     /locus_tag="IV328_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgR"
                     /protein_id="HAP8938953.1"
                     /translation="MKLKPRNLKQITCEFVLFNRQHIKFSNQTIKRKITDFSGKEIIL
                     YAKKLSNEQELVCFFDQIKAVYVDGKIVACRGCSELPLIVSNHVFAKMKARQNLLRES
                     KIRYREIQKEELEKRKSEVRKKYKRISA"
     gene            complement(2876..3919)
                     /locus_tag="IV328_002895"
     CDS             complement(2876..3919)
                     /locus_tag="IV328_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8938954.1"
                     /translation="MSEFKYYKSKEVYREKYYQMPKVFFTNEKYLNLSNDAKITYMLL
                     KARFDYSVQNNWVDSEDNIYFIYTIEELMRLLNCREGKISKIKKELEAAGLLKQKKGR
                     IKKANGKIETTPNLLYLGKPEVTSEDVFKINEEERLIPNPVNAKIANTEKDDDIKVIP
                     VNAKIANTEKASDTNSSPVIAKIADNILYSNNSLDTNRHNTDTDKDQLQDQLLLDNFV
                     DIMTDSSFDTFVPTNVLNLIKTFSNSYSEAQQTVKTIHNAKHKAQESENTIIVYEEIV
                     YYGVDADKGLYMTLLKAYQKQKTEKVDNLQNLIFIYVKNWFAEKAIPAKLAHEQNTET
                     NPLPAVNLENWLD"
BASE COUNT         1187 a          685 c          539 g         1523 t
ORIGIN      
        1 tttgtaagag tgatttaaat actgcttgcc agcaagtatc atattgtgac aaatcatatc
       61 catatttatc taaccattta ataaaagtat cgtaagtaat gctacgacca atatttttct
      121 tagcatattg cataaccaat tttttaaatt cgggtttatt agaatataat actttctttt
      181 tttcttcgat cattttattc tccttaataa attaaaatgt tgttttgttt atccgttact
      241 gcaattttga tatttaagtt accatactct ctatcttgtt ttatttgcac tgactgatct
      301 ttgatccatt tgatataatt atccaagttt gctacttggt tcttcctttt catatcactt
      361 agaaattgaa gaagctcttt ttcatttttt aaccattcca taccaacacc tcataaaaat
      421 taatttattt ttcttctaga gcaatttttt gcagttttaa aagagtaacc cacgctttat
      481 ctactttttc gttattcctt ttatcaatat aaaaaagttt gctagttaca ctttctggat
      541 attttaaaag tggatataaa tcccaagtca gatattgaac tcttcttaaa gatacttcat
      601 gagattcttt aggattataa tttctatgag tggctaaatc atattcaaaa tacacttcgt
      661 caattgtgtt atcgaagtaa ctttgttcgc attgaactct catacctccc catattccca
      721 aagaaaaaaa tactagagct atacccacat ttaccttaaa aatacttgag aactcgtttt
      781 tattactttt accaaagcga atacgattca aatttctcag ctcctttata tacttgttaa
      841 ggaattaaag aagtaaacta ttacttatga attgccaaaa ctctttctta tcacatattc
      901 attattgatc aattcagaaa gtgtatcaca aggataaata cgttttttac ttcttttttg
      961 gtgctctttt actagccaat ccaatttttc tatagtttca tcagaaaatg catacttttt
     1021 tctttttttc tcagtactca taggtgtacc ctccagttaa attaggtgtc cccttatttt
     1081 ttagatactt gattcttttg tttattagtt ttctcataaa tgttacagtt tttttacgca
     1141 gggtttctag cgatagcaac cccaaactct cgaaaagtgt ttttgctccc tagaaaaacg
     1201 agcggacggt tttaatcgtc atcttatgtt tttcgtgata gctgatcatc gcaaataacc
     1261 ttttctgtgt gtgccctacc catcaataac gtaccatctg ataacacagc cgtacttttt
     1321 taggtggttt atccttccta agcccctatt agacacgctg attaacttag aaagtcctgt
     1381 aatttttcag ttgtcaagga acacccaaaa catcaaagta agtttacgtg tatcgcttag
     1441 tgtcatgtga ctccttgcca gcaaacgtta aataaatatt tagcgctgtt tcgtgtccgt
     1501 gtgcttactt gtccgttttt tcgatagcaa taaatccctg ctattaggtt gtttgttaga
     1561 aaaaaactaa caaacaaaaa aatcaagtat cattagtcaa aaaatttgct aagcactaat
     1621 tcgtttatat tttttgcgta cctcactctt tcttttttcc aattcctctt tttgtatttc
     1681 tctataacga attttcgact ctcttaataa attctgacgt gctttcattt tcgcaaatac
     1741 atggtttgaa acaattaagg gtagctccga acagcctcta caagcaacga tcttaccatc
     1801 aacatagaca gcttttattt gatcaaaaaa gcaaactaat tcctgctcat ttgataattt
     1861 tttagcgtat aaaataattt ctttacctga aaaatcagta attttacgct tgatagtttg
     1921 attagaaaac tttatgtgtt gtcgattaaa cagtacaaat tcgcaagtaa tttgttttaa
     1981 atttctaggt tttaacttca tgtttttctc cgcctttctg cttcagttgc tgatcattgg
     2041 gaagccaagc acgacaaaaa gagggcaatc acacatgaag tgtaattgcc ctcttaagtc
     2101 acaaatcagc tgcaaacagt ataaacaaat atatctagaa aattgcacac taagtctact
     2161 caaaaaagta gccagagaaa tacaaatttt cttttaaaga atttaggtag cacaaataaa
     2221 ctagttaaat tcttaagaaa tatatgttag aatatgcctg ttgcagttaa atctgcaaca
     2281 aaagtggcag tttacctctt cagcgtggta catacgttga attgaggacg gctagcgatt
     2341 catgacctga agtaagagtt ggcgctctta cccagcgaga tcactagtcg gtctgctctt
     2401 ttttgttggt tgatttattg cttggcttct caatgatcag cagtctggat tgctaatcaa
     2461 taattttttt aatttacgtt tctacaaatt tacggtagca catcttcaaa ttcattacaa
     2521 gcttttttat ttttcacttg acaaatttgt gtaagtacta attatcgtaa aacaacataa
     2581 aacaattata ttttgatata caccacctcc actcacatag ttgtgaagcg cttctggaat
     2641 tattttaaca tttaaaatca ttgataacaa agcttacatt acacacttta cacgttgttt
     2701 attagagata ctatcgttat cgaataaaga acactcatga aagttttaat gaaaatttag
     2761 aaaccaaata acatgattta ttctaatttt ttctttgtta aatgatcatg caatatatat
     2821 cacaataaaa ttataccact catcgcctat attgcaaaca tatgttcgtt tttttctaat
     2881 ctaaccaatt ttctagattt acagcaggta atgggttagt ttctgtgttt tgttcatgtg
     2941 ccaatttagc aggaatagct ttctcagcaa accagttttt tacgtaaata aaaattagat
     3001 tttgtaggtt atcaactttt tctgttttct gtttttgata tgccttcagt aaagtcatgt
     3061 acaatccttt atctgcgtca actccataat acacaatttc ttcgtaaaca ataattgtgt
     3121 tttctgattc ttgagcttta tgctttgcat tgtgtatagt tttgactgtt tgttgtgctt
     3181 cactgtaact atttgaaaaa gttttaatta aattcaaaac attagttggt acaaaggtat
     3241 caaagctgga atcggtcata atatcaacaa aattatctag caataattga tcttgtagct
     3301 gatctttgtc agtgtctgta ttgtgtctat tagtgtctaa agaattatta gaatataata
     3361 tattgtctgc gattttcgca attacaggag aactattggt atcactggct ttttctgtat
     3421 ttgcgatttt cgcatttaca ggaataacct tgatatcatc gtctttttct gtatttgcga
     3481 ttttcgcatt tacaggattt gggattagac gttcttcttc gtttatttta aatacgtcct
     3541 ctgaagtcac ttcaggttta ccgagataca aaaggtttgg tgtagtttca atttttccgt
     3601 ttgctttttt gatacgtcct ttcttttgct tcagtagtcc tgctgcctct aattcttttt
     3661 ttattttaga aattttacct tctcgacagt ttagtaatct cattaactct tcaatggtgt
     3721 aaataaaata aatattatcc tcagaatcta cccaattatt ttgtactgag taatcaaaac
     3781 gtgctttcaa aagcatatag gttattttcg catcatttga taaatttaaa tatttctcgt
     3841 ttgtaaaaaa tactttaggc atttgatagt atttttcacg ataaacttct tttgacttgt
     3901 agtatttaaa ttccgacata atattactcc tttt
//
LOCUS       DACDKJ010000134         3765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDKJ010000134 DACDKJ010000000
VERSION     DACDKJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.141"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1379
                     /locus_tag="IV328_002896"
     CDS             <1..1379
                     /locus_tag="IV328_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8938955.1"
                     /translation="EWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNLETFQH
                     SAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRWFAFPR
                     LVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSKKNNKH
                     KVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNTTFENL
                     TLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAILVK
                     VTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIRA
                     VRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGYAQVYG
                     KYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLAEIA
                     FPNEKIFR"
     gene            1395..2435
                     /locus_tag="IV328_002897"
     CDS             1395..2435
                     /locus_tag="IV328_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8938956.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2454..3539
                     /locus_tag="IV328_002898"
     CDS             2454..3539
                     /locus_tag="IV328_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8938957.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3572..>3765
                     /locus_tag="IV328_002899"
     CDS             3572..>3765
                     /locus_tag="IV328_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8938958.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIY"
BASE COUNT         1349 a          536 c          698 g         1182 t
ORIGIN      
        1 gagaatggag tgctgcacga cggatattca ttatattaat ggatgttatt gtatacaatg
       61 cagcaatata tagtagtttc ttactgaagt ttcgtggaga gatcccatta cgaaatttag
      121 aaactttcca acactcagca attattattt cagtgatttt tattgcgttg aacattttgt
      181 tgggtgcata tgttttttat aatcgaatga tcagtgacat cgtgtttgtc acagtcatcg
      241 gtcaggtatt gatgacacta gggatcatgg tagtgacttt tgcgggccgc tggtttgctt
      301 ttccacgtct tgttatctta ctttcatttg ttctcggtac gcttttctta agtatctatc
      361 gtattctcgt atataaactt tatttacgcg taagtcgaga taaaaaggtc gtcattattg
      421 ggttagaaaa ggatgtcgca ccagcaatcc aaaattttgg ttcaaagaaa aacaacaaac
      481 ataaagtcga agcagtcatt cttgataact attatgcaaa catcaaaaat atgattaaag
      541 acatcgatat tgtctatctt gcttctcata tcgaagaaac tgaaaaaatc aaaatctatc
      601 aattagttac taaaaatgaa aaaaaattgt ttttgaatac aacatttgaa aacttgacat
      661 tgatcaatcc aaatattatg aatttcgaag atgaaagtat tattgaagca tctggattta
      721 gaatccaacc tgaatatgaa ctattcaaac gtttgtttga tatcatcgtt tcacttgttt
      781 tattagttat tgcttcacca tttatgttat tcacagcaat tttagtcaaa gtgacatcac
      841 cgggaccaat catctataaa caagtgcgga tcacaaaaaa tcaaaaggaa ttttcaatct
      901 ataaattccg ctcgatgacc gccacagccg aagcaaaatc tggaccagtt ttagctaaga
      961 gcaatgatgc acgagtaacg cctgtaggta aatttattcg agctgtccgc tttgatgaat
     1021 taccacaaat cttcaatgtg ctgaaaggcg atatgtcgat cgtaggacca cgtcctgaac
     1081 gttctttctt cgtcgatcag ttcaatgcag aaaaccctta ttactactta cggcataatg
     1141 ttcgtgctgg tatcactggg tatgcacaag tctatggaaa atatgtctct gattacaaca
     1201 gtaaattacg ttttgattta ctttatatca aaaaatattc attgatgctt gatttaaaga
     1261 tcttgctgca aaccatcaag atcctatttg ataaaatgtc ctctcaagga cttgaagaag
     1321 acgaaaatgt gatctcattg gcagaaattg cttttccgaa tgaaaaaatc tttcgctagt
     1381 tgggaggcgt aaaaatgaac aatccgaaat tcagcataat cgtaccagta tacaatgcag
     1441 aagcaactat tggtcgaacg attgatattc taaaagaatt ggattatgaa gagtacgaag
     1501 tcgttttagt aaatgatgga tcaacagatc aaacacagga aatcattcaa gaactgattg
     1561 aaaatgatga acgttttgaa ttgattacta cgaccaatca agggcccgga ttagcaagaa
     1621 acgaaggaat aagacagtca aaaggagaat atctcttatt cttcgatgca gatgacacgc
     1681 cagtaaaaac gatcttgtca gattatgatc atttgctaga aaagagtcca aaagcggatc
     1741 ttgttgttag ttcgtttgtt tttcgtacaa tggacgaggg aagaatcgtt tccgaaaaag
     1801 aaaacttagt tacagaatgt cactatctaa ctcacgaaga gtttataaaa gacatgtatc
     1861 agttaatgaa tgaacagttg atgtatgttg tatggaacaa atgctatcgt agcgatattc
     1921 tgaaaaataa ccaaattcta tttaaaactt ataatagttg tgaggatcga atatttaact
     1981 tacattatta tgaatactgt catcaagtga tcatgaaccc aaaagtagaa tatatttatg
     2041 aatttgaagg tgggaagggg atcactaatc aataccgacc taacaagttc gaaacattta
     2101 aagaattcta ttcattagca aatactgtaa cagacgatat taataaagac ggtatggcgg
     2161 cgcttctttt aaaagggaca acttcagtta ttttttctat ttatagtacc tctgcaagaa
     2221 cttcaaaaga gaagaaagac gaagcaaagc agatattgag tgatccagtg atccgagaag
     2281 ccaagcaaat tgctaagacg gattcttcag caaaaaaaat aacaaagttg ctttacaata
     2341 tgccagctcc actattttta tctgctgtaa aagttggaag cttcgttgaa gtgaaaatgc
     2401 cagggttaat ggctgctttg aagcgagtat attagttaat ggaggaaaat ataatgaaaa
     2461 gaattttgca ttttcaggga cgaatgggtt taggtggagc agagtctttc atgatgaatt
     2521 tatatcgtaa aattgatcgt acaaactatc aatttgattt tttgatttat gaagattatg
     2581 cagatgttca agactatcat tcagagattg aaagattagg aggccgtatt tttgttgtac
     2641 ctaatcctaa gaaaaatatt tttaaatatt taatagaagt gaataagtta ctaaaaaaag
     2701 aatctttttc tatagttcac aatcaggttt attttggcgg gggaattaat ttatggttag
     2761 ccaaaaagaa tggaatcaga cagagaattg ctcatagcca tgcaactgaa gatggaaaat
     2821 ctcaaaatat tgtaatgaat gtattgcgta aatttttaac caaattgttg ttacaaaatg
     2881 caacagacta ccttgccgtt agtcaagaag ctggtgaaag tctgtttcaa aaccatccct
     2941 ttgagatagt tcataatggt attgatttag agctttatag taaaaattca gaagctaagg
     3001 ttaataagag aaaagaattg gatatctcaa tgagtacttt tgttgtggga aacattggac
     3061 gattagagaa acaaaaaaat caatcttatt tgttagagat ttttttagaa atactaaaaa
     3121 tgaattctga ttcttacttg attttagtag gtgaagggtc acttagaaat aaactgaaag
     3181 ccaaagcagt agaattggat atagagaaaa gagtaaaatt tttaggagaa agaaacgata
     3241 ttcccgagtt attatctgtg atggatgttt ttgtaatgcc ttctctttat gaaggtctgc
     3301 caatggttgg attagaagca caagcagcaa aattaaaatt agtcttagca gatactattt
     3361 ccaaagatac aaagttaaca aataatgtgg attttgtatc attagatgaa acaccacaag
     3421 aatgggcaaa aaaagtaata aaagagccgt atgctaataa aataacacaa gaactaaaga
     3481 gttatgatgt ttcgtacact actgaacaaa tggagagtat atatagtaga gatcattaaa
     3541 aatgttcaag atattttagg agtgattatt tttgaaaata tgttatttga tattggcata
     3601 caatgatatt ggcaacttaa agagattggt caatcgattg aacgaaaatg cagtttttat
     3661 tattcatata gataaaaaaa ctgatataga accatttatt gaagcattta aatcatatga
     3721 taatgttgaa tttttatgta ataaaaaccg tgttaaaatt tattg
//
LOCUS       DACDKJ010000135         3714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDKJ010000135 DACDKJ010000000
VERSION     DACDKJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.142"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IV328_002900"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IV328_002900"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8938959.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IV328_002901"
     CDS             complement(2261..2512)
                     /locus_tag="IV328_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8938960.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IV328_002902"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IV328_002902"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8938961.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IV328_002903"
     CDS             3462..3680
                     /locus_tag="IV328_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938962.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1026 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aaga
//
LOCUS       DACDKJ010000136         3494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDKJ010000136 DACDKJ010000000
VERSION     DACDKJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.143"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            245..1285
                     /locus_tag="IV328_002904"
     CDS             245..1285
                     /locus_tag="IV328_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8938963.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2109..2441
                     /locus_tag="IV328_002905"
     CDS             2109..2441
                     /locus_tag="IV328_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8938964.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3060..3401
                     /locus_tag="IV328_002906"
     CDS             3060..3401
                     /locus_tag="IV328_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8938965.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1299 a          466 c          653 g         1076 t
ORIGIN      
        1 ttaattttaa aactaaattt tattcatacg tttttctggt agtatttaat tataaataat
       61 atttatttaa agtagttctt aatgacttta aaggtagtac ataattcatt ttatttctga
      121 ttttaggtag tatttaatta aaaaacagtt ttaatgcgtt tgcatttttt atttatcttt
      181 gctattttcg aaaagagtag gtaattttac ataaataaaa caacgtgaaa gtagaggatc
      241 tacaatgact gattttaaat tttttaaggc ggatagagtt tacaacgaat tattttatca
      301 atttccaaaa gtctttattg tttctgacga atacaaaaaa atgaaagatt caactaagat
      361 tgcctatatg cttttaaaag caagattaga gatcgcaatc agcaaacggc aaatcgatga
      421 agaaggtaat gtttatttta cttatacgac aaatgaacta tgtagagtat taaactgcca
      481 aaaacaaaaa gcgatagcaa tcaaaaaaga gttggaatcc tttggtttat tattacaaaa
      541 gcagatggga tttaacaaac agttagggaa aaataatcct aatagactat atctagcaga
      601 attaaaagtc tcagaaaatg atatctactt actcgaaaaa tttgatagag agaataggga
      661 aaacgttgat aaatcagagg gtatgaaaat catacccacc ctcgacgaaa aatcagacgc
      721 tgaatccctt ggggctcaag agggtatgaa aatcataccg tgccaaaacg ttgataaatc
      781 agagggtatg aaaatcatac cagaacttaa taataatata ttagacacta atagacacaa
      841 tatagacact gaaaaagacc gcctacaaga tcaattgttg ttagacaatt ttgagacaat
      901 tatgacaaac gacagcattg ctacgtttgt ccctgaacga gtattaaatt tgataaaaac
      961 attttcttca agttacagtg aagctcaaaa aaccgtccag actattcata atgcaaagaa
     1021 aaaagctgaa atagaaagtg gtatttcgat agtttttgaa gaactcgata gttattatgt
     1081 caatgcagaa caagaattat acacgacact gttaaaagcc tatcaaaaat taaaaaccga
     1141 aaaagtcgaa aatatccaga acctgatttt tgtctatgta aaaaattggt ttatcgaaaa
     1201 accaatagct gctaaagtat caagtgaaaa acgtttgaat tatgaaagct ccccaagcac
     1261 tattacgaaa gactggttag agtgaaactt tactgacaaa gtgattgttg cttcatggag
     1321 taacattaaa attaaaaatt ttttattatt ctaattatat tacgaatatt acaaaataat
     1381 agctatttta atagaattta taagttaaaa tattatgata aaaatcacaa acgtagcctt
     1441 ttacgtcata agtgttaaaa gaaataatta atttcaaata tagaaaaggg aacgtatgtt
     1501 tgcatactcc ttttttttga gttataataa tacgcgaaga gagataattt cattatttaa
     1561 acaagctaaa aatcttgata ttacttcttt tcaggcaata cgaatattat tcgcttacat
     1621 tttttgtata aatttaattg tctgctatgc ttgtgatgaa ttgattttcg ttctgcactt
     1681 ttggatgata aatttggaca attgaaaata caaaaaaaag aaaggcggta tgaactcgga
     1741 aggctggtaa ccttcgtcag cgttcataca cggcattaag atttggccct taatgcgtta
     1801 acctttcagt gttacggtat tacccgcaat ggatttagta taacagatat tcaaaagtga
     1861 taccactttt tttggctgtg ttaaggttaa aaatacgtga tattacgatg aatctatgcc
     1921 taagcacaat gttataaaaa tgtccgtgtg gctctttgcc gtatgacgaa cgctgaaatt
     1981 agagggaacg catgtttgga tgcgttccct cttttttgtt tgtcatgaac gaaaatcaat
     2041 tctctatttt tgaaaaataa caagaaaaac ttgttaaaaa tatttaaaga aattgggtgg
     2101 tttattttat gtcatataca ggaattttga gtctcaaaga tatttgtcac tacggtaaac
     2161 ggtgtaccgc tacagagaaa atcacgaaga aactatcaac tggccaaagt aaaaccgttg
     2221 tgcagtgtaa gaaatatata atccagaaag acaaagtctc agaggaaatg atttattaca
     2281 ttgggaaaca aaagcaaata attttaaaag atcctatccc cttaaaagag ctatatccaa
     2341 caatcaaaca tgtttatgac caaaacggtg tactgattgg tcgaagaaaa aatggagtct
     2401 tgcgttgtac cgcaaaaggg atggggcgtt tgatcggctg aattgatcat tctcataact
     2461 tgtattttat cgtttttcgc ttgcttgaaa atattgtcga gacttctaaa ggaacttaga
     2521 gtcactagaa aaataaggag tagacttgat ctatcaacaa ttcccttcgg ggagtgacgg
     2581 ggtaggtttt aacgattggt aacgtaaaaa attggtcaat cgagtaaatg cgcttgtttg
     2641 gatagtggtg aaatgaattt gtgtaaataa taagcgcaga gtaaacaaga gggaatttag
     2701 aagttagaca agttagaata gcgtctagat taatcatgag cagctactaa gggatctaga
     2761 ctttgacgca aactcatgga ataaggagta ggtcttctcg taatggggag gacgcacaag
     2821 aaattgtgtc gtgtgctgca gtacatgtag atacttgcga ggacgttgat tattggatca
     2881 atcgagcatt taccgctgtc tactagggtt tctcgtttcg gacgccataa acgagtcgaa
     2941 attttttatg tatttttaag taaacgaaaa acgataaaat atgaacaagt cctgaataaa
     3001 gtgaaaaaat tgatataaaa catagagaaa atgaaattta cgtaatggag gaatgacaaa
     3061 tgaaaaatta tgatccgaat atacgattgg gcacacatac tattaaggtt tctttccaac
     3121 gttgggatta caaaggattt gtgacgttta gaagaggcgg aaattgtaaa ggattagatg
     3181 ttttagcatt agacgaggat gatttatacg atcaaacatt gactgataat ccaattggat
     3241 tcggcttgct acctgaagat gatgaaggta atgaatggtt taaaatgacg cttatgaatg
     3301 ataatggaga cgaattatct gttgaagata cttggtcata tttgagtgat tatattgtca
     3361 gctttgaaat cattgaattt gtagcggata aagaagaata aaaactggag gaataaattt
     3421 tacagataaa ggcttagaag gagtcacgat gattaataaa aaggtagtat taccttaggc
     3481 attgagtaag gaat
//
LOCUS       DACDKJ010000137         3391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDKJ010000137 DACDKJ010000000
VERSION     DACDKJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.144"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(22..2493)
                     /locus_tag="IV328_002907"
     CDS             complement(22..2493)
                     /locus_tag="IV328_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8938966.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2766..>3391
                     /locus_tag="IV328_002908"
     CDS             2766..>3391
                     /locus_tag="IV328_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8938967.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          999 a          686 c          609 g         1097 t
ORIGIN      
        1 tcttttttga gatacttttc gtcaattttc tcgtttaaat atcgatagaa attttttagc
       61 tacgcgacta aacggctttt gctctttttc atttttacgt tcaaacaaat attctaccaa
      121 tgtggtcaaa tgcctaaaaa tcgaatctgc cgagaaaaat agaatgctca acagcaaaag
      181 tgatacagct gagataccgg tgattgaaaa cagcttatgc aaaaagatga ccgaaaaaaa
      241 gttaccgcta gcacagaaaa gaatgatctg acttctgaat ttattcagcg gtgaacagat
      301 tttatagaga acgaggaacc ctactgcaat caaaagcatg gtagatgctg tagaaacatc
      361 tgttttattt agattcaaag tcaccccgca gatcaccaac gcaccgacta cgatcatatc
      421 tgttagtcca cctggcacag ctttttccat cacattcatg atgaatttcc ctctgattcg
      481 ctttttgttt tcttctagag caagcaagaa agatggaagt ccgatagtaa acaaactaat
      541 caatgtgatt tgtgatggtt ctaaaggata ggtcagagca aagatcactg aaaacagtga
      601 gagcaaaaag gagaaaatat tcttcactaa aaacaagctg gctgatcgct caatattgtt
      661 cacaacgcgc cgtccttctg caacaacttc tggcatgcgt gaaaagtcag aatcaagtaa
      721 gaccacttgt gaggcctgca tggcggcttc atttccggaa gccatcgcta tactgcagtc
      781 tgcttctttc atggctagaa tatcattcac gccatcacct gtcatagcta ctgtattgtt
      841 acgctttatc aattgaacga agattttctt ttgttctggc ttgacgcgtc caaaaactgt
      901 atatttttcg acagctgctt catattcttc ttctgataat ttagatgcat caacgtactg
      961 atcagcatgt gaaatccctg cttggacagc tacattggat acggtgagcg gattatctcc
     1021 agaaatcact ttgatggcta cttgctgttt tttgaagtaa tcaaatgtgg ctcttgcttc
     1081 ttttctgata ggattagcaa tcagaatata gcctaatggt gtgactgctt ctgtcaatac
     1141 tggttcttca agaatcccat cgtacacacc aaagaccaac acgcgatagc ctttttcagc
     1201 gaaagaaaca aactcttccg catattccgg caattgatcg cgcaaaacca tttcgggcgc
     1261 tcctaaaaca tacaccttgt ctgccaacgc tacactgctg aatttttgga cagaagaaaa
     1321 aggaataaca tggtgatagg attgatcagt ggtttttgta aaatatgctt taacagcttc
     1381 cattgttgca ttgtctcctg ccatcgcttt tgcatagtca gcaatgcatg cttccaattg
     1441 atctgactga ctgattattt cttgtttttt agagacgatc attttttgaa cagacatttt
     1501 attttctgtg atcgttcctg ttttatctac acaaaggaca tttacccttg ccaatgtctc
     1561 aatgcttttc atattgtgta aaagaactct ttgctttgct agtttagtcg cacttaatgc
     1621 taatgcaatc gtcgtcagca agtacagtcc ttcaggtatc ataccgatca atgctgcttc
     1681 cattgcaacg acactttctt tgatcgtgtt tccattaaaa aagtaacttt gagaaaaaag
     1741 caggataccg ataggaataa tgatgatccc aatccatttg atcaattgat taagagaagc
     1801 aatcatctcc gactgttctc cttctcccat tgtcttggct ttgactgtca gctgggaaat
     1861 atacgaatct gctccaactt tgtctaatcg ggcgtaagct tttccggaaa caatgaaact
     1921 gccggacatt aatggatcac ctatttcttt ggttatttca tctgcttcgc ctgtcaataa
     1981 agcctcatta acttgcagtt tcccttccac gacctctgca tctgctggaa tttgatatcc
     2041 agtctctaaa ataacaatgt catctattac aaggtcttct atatctattg tttgttctat
     2101 cccgtctcgt atgacattga cttttgctgt atttaaaaca tttagttcgt ccaagatctt
     2161 tttggatcga atttcctgaa aaatggcgat tcctgtattg gctatgataa ctggtaaaaa
     2221 agtcaagttc ttataggatt ctactaaaca aagcaaaatg gctaagataa gaaagatcaa
     2281 attgaaatac gtaaatatgt tttccaatac gatttgctga tttgttttga atgtgggatc
     2341 gactgaatta tttgctgcac ctttttctat tcgttccttg acttgatctg ccgttaatcc
     2401 ttctttatac gatggaaaaa gacggatgac tttatctgat gatctttgtg ttactttctt
     2461 ttttttactt cgatttatcc atttattctt catgtggcta cctcgattga tttcgttttc
     2521 tggatatttc caaacttctc ctatccagta tagaacagat atggaaagtg ttcctcacat
     2581 taagcttgca aacacctttc attcctgtca tatagcaaaa aaaaagggtt ctataatatt
     2641 ttgtgttggt ggaaaatcga aggcgatttt ctactaacac tttttcttcg tttcaactca
     2701 aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcatcctac caaaggagtc
     2761 tctcaatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
     2821 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggaa
     2881 aattggatga ctcttcttcc tattgtgaac actgtcgatc ggttcgtatt atccgcaacg
     2941 gttcctatac gacacgcact cagatgttga aagtcaaaga aaagttaact attttagaat
     3001 taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg aaaacggact
     3061 tagttgatga acatcatcaa ctaacaaagg aactgaagca ggtgattctc atggaactgt
     3121 acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga
     3181 cacggatctt gcgtgaagca acgaagcatt accagccacg tatgaacttc cttccgacgg
     3241 tgttgtgtat ggatgagttc aaatcaatga attctgtttc tggttccatg agttttattt
     3301 gtgttgatgg gacaaccaat cagctattta cgatccttga agatcgcaga ctttacaaac
     3361 taactcaata cttcctgcgc tttcctagaa a
//
LOCUS       DACDKJ010000138         3329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDKJ010000138 DACDKJ010000000
VERSION     DACDKJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.145"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..290)
                     /locus_tag="IV328_002909"
     CDS             complement(<1..290)
                     /locus_tag="IV328_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8938968.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="IV328_002910"
     CDS             complement(283..624)
                     /locus_tag="IV328_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938969.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="IV328_002911"
     CDS             complement(798..1523)
                     /locus_tag="IV328_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8938970.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="IV328_002912"
     CDS             1576..2052
                     /locus_tag="IV328_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938971.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="IV328_002913"
     CDS             complement(2058..2240)
                     /locus_tag="IV328_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938972.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="IV328_002914"
     CDS             complement(2240..2539)
                     /locus_tag="IV328_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938973.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="IV328_002915"
     CDS             complement(2644..2847)
                     /locus_tag="IV328_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8938974.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..>3329)
                     /locus_tag="IV328_002916"
     CDS             complement(2862..>3329)
                     /locus_tag="IV328_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8938975.1"
                     /translation="SEVLPTIRKTGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEY
                     EPKISYLDTILSSTDTVATSQIAADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMN
                     QGYTKSHTSEIPKADGGTKVVMNTKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
BASE COUNT          905 a          668 c          508 g         1248 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcact
//
LOCUS       DACDKJ010000139         3128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDKJ010000139 DACDKJ010000000
VERSION     DACDKJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.146"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IV328_002917"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IV328_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IV328_002918"
     CDS             1360..1992
                     /locus_tag="IV328_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8938976.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IV328_002919"
     CDS             2197..2424
                     /locus_tag="IV328_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP8938977.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IV328_002920"
     CDS             2493..2759
                     /locus_tag="IV328_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8938978.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IV328_002921"
     CDS             2749..3018
                     /locus_tag="IV328_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8938979.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DACDKJ010000140         3087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDKJ010000140 DACDKJ010000000
VERSION     DACDKJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.147"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IV328_002922"
     CDS             complement(25..144)
                     /locus_tag="IV328_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8938980.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IV328_002923"
     CDS             complement(159..1343)
                     /locus_tag="IV328_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8938981.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="IV328_002924"
     CDS             complement(1602..1892)
                     /locus_tag="IV328_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938982.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="IV328_002925"
                     /pseudo
     CDS             complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="IV328_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          842 a          739 c          540 g          966 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
     3061 acattttgct cttggctcat acattat
//
LOCUS       DACDKJ010000141         3080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDKJ010000141 DACDKJ010000000
VERSION     DACDKJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.148"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..257)
                     /locus_tag="IV328_002926"
     CDS             complement(<1..257)
                     /locus_tag="IV328_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8938983.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSA"
     gene            335..643
                     /locus_tag="IV328_002927"
     CDS             335..643
                     /locus_tag="IV328_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938984.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(614..871)
                     /locus_tag="IV328_002928"
     CDS             complement(614..871)
                     /locus_tag="IV328_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP8938985.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            1603..1923
                     /locus_tag="IV328_002929"
     CDS             1603..1923
                     /locus_tag="IV328_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8938986.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1941..2372
                     /locus_tag="IV328_002930"
     CDS             1941..2372
                     /locus_tag="IV328_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8938987.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            2499..>3080
                     /locus_tag="IV328_002931"
     CDS             2499..>3080
                     /locus_tag="IV328_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8938988.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
BASE COUNT         1027 a          494 c          532 g         1027 t
ORIGIN      
        1 ttggcagagg gaagttttga ttttaagatt aaactgtaaa ggcctgactc gttaatcaaa
       61 acaactgctg ttccattgac ggtgaacgaa tcgttctgcg tcttatcctc caagtccaca
      121 tgatctcgaa tagctttttg cggattcgaa tatcctagaa cgtctgctac atcttttcct
      181 acaaaatatg gttcatcatt tactaaaata gttcgaactt cgttttgctc gaaattaaaa
      241 atttgtggtg tattcatatt tctcattcct ttctttggta taatttcctt atcagtgtga
      301 caggctgaaa taattgttaa ggaggtgaaa atttatgaaa ccagaatttg taattactat
      361 gcttgatgga gaaaaaatca aaatatttgc cgatactttg gtcgttggat acgatgatgc
      421 gtcaaatatc gaagaaaaag atggaatgtt ctattcttct caaatttacg tgaatacctt
      481 gcaaggtgat attggggtat ctactcttgc aacatcaaga ccgcttgtag gtcttatcgg
      541 ctttttgtca aaagcaacat tcttttctct cggcgatgat atagattcgg atgaaactgt
      601 catctataaa acatcagctg ttaaaagtat cggtttcaaa tagtaattct tgctcgttga
      661 tagcggaagc caacttttct tccaacacat cggcgagctt tctttttgtt ggattcattt
      721 caaagttgat agtcttgaga agcatttttg catccttaac tttttgtcct tgcaattcct
      781 tgatgatttt atcggcttgg ctatcaattc catgtgttag atcatctatc attttttgta
      841 ccttgattgt ttggctcatt tccttgttca ttgtttagcc tcctttaata aatgattttt
      901 tgtatttttt aatttccata aattcctcaa attgctcttt ggtattatta ccacgaccat
      961 acatattgtg aaattcttta tggcaactga cacaaagtgt aacgccgttt gaaatcgctg
     1021 cggatttagt tgttgcatgg ctaaatcacg aatcacaagt atctactaaa ggcgaatcaa
     1081 tttcgccaaa agaaattgtt caagcttatt tagatattca ttatgctgtt atctatggtc
     1141 agcttccaga agaccgtaaa aacgatgact aaacgtgagc taaaatctct gctatggctg
     1201 caaccatggc agagtcttct ttatctaccg cgtgttccat cgccactttt gcttgtttca
     1261 gtatgtctaa tttcaaatct tcgatttttg ccgagactgt tttttccata ttcttctctc
     1321 ctttctttta tattcgtaaa caaatttaac aactttttaa aaaactgcgt tgactaaatt
     1381 aacatacagt gttaaaatca gaacatagtt aaataagaca tataaacatt gattttaaaa
     1441 gctttcttgg cggttggcat ttattaatca atagtgtttt ttgttgtctt tttagttgtt
     1501 aaacttgttt acaagaatga tattaacacc ttttgttaaa atagtcaacg aattttaaca
     1561 aataaagtta aatatttttt cttgaacgaa aggaatattg atatgataac gtttgagaga
     1621 ataaaagagt tagcgaaaaa acagggaaaa tcattaaata aagttgaaga agacctaggc
     1681 tatggtaaaa acgtcttata tagacttaag aactcaaacc cctctacaga acgtttgcaa
     1741 gaaattgctg actatttcga tgtctctgta gactatcttc tggggcgcga agaaaaagaa
     1801 acccctaaac atgtggattt atcagaaaac gatactgttt tttcttttga tggaaaagaa
     1861 atatctaagg agacaatgcg taaagcgatt gcaattgcta aagctttaga ggaaaatgaa
     1921 tagttggagt gatgggttgt atgtatttga aattgaaaga aatgttgagc gaatacaatt
     1981 tgaaagtagt ttacatggaa atgaaagagc ctggcttcta ttatccgaag cctagaatca
     2041 tttttttgaa tgaaaatcta tatggcgaaa ctgcagaagc ctttcattta tctcatgaac
     2101 ttgcacattt cagcgcctct cattttgaat tttcagtttt gtacgatact tctacaactt
     2161 ttcactcaaa atttgaaaca gaagcagata aaatagctat tctaatttta ttaaatatct
     2221 atattgagaa cgaactgact gatgaatctc agtttaacct cgaaaaattt atggaatatt
     2281 attctattca gaataagctc agatacactt gttatgctgt ctgccaatgc tattttaaga
     2341 aaaaatattc ttacgcaagg caatatgtat gaatacatca agaattatta aaaaaaccga
     2401 tttcagcaaa acattaaaaa agcccgtgct ggcacacgga ctcatacctc atttctgaga
     2461 tcgcaaatat attataacaa gaagtgagga atatttaaat ggcaaaaaaa gttatgggtc
     2521 aagacggaaa aatgtataaa gttagtaaac ctttttacaa aaaagtatgg ttttgggtat
     2581 tagcagtaat tttaattata attattggtt ctgctttaaa tggtggatca gatagtaata
     2641 aagcaagtga taatggtggc gaaaaagtaa ctaaatcttc aacctctgct tcatcttcta
     2701 aagaagaaaa aagcgatacc ttctataaga ttggtgacac tgtaaaagtt ggtgatgctg
     2761 aatatacact aaatagtgta gaactaactg atgaaagaaa tcaatttgaa gaaaaccaac
     2821 ctgcacaagt agtaaaaatt acttataccg taaaaaatga tggcgattca gatatccctg
     2881 taggtacaga tgtggaagta tatggaccag atgacaaaaa atcagagaca tatgccaacg
     2941 aaaatacaat gggatctgtt gctccaggaa aacaaatgga tgtaactgct catttcacgt
     3001 taaaccaaaa gggagaaata gaaatccact tctctccttt agtatcattt gaaaaagcag
     3061 ctatttttaa agcaactgta
//
LOCUS       DACDKJ010000142         3018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDKJ010000142 DACDKJ010000000
VERSION     DACDKJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.149"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..384
                     /locus_tag="IV328_002932"
     CDS             <1..384
                     /locus_tag="IV328_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP8938989.1"
                     /translation="SNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVV
                     RDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMR
                     DELSELAKKVKVTFLTDPRWVRIDK"
     gene            519..1097
                     /locus_tag="IV328_002933"
     CDS             519..1097
                     /locus_tag="IV328_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938990.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            1043..1561
                     /locus_tag="IV328_002934"
     CDS             1043..1561
                     /locus_tag="IV328_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938991.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            1569..2609
                     /locus_tag="IV328_002935"
     CDS             1569..2609
                     /locus_tag="IV328_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8938992.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            2858..>3018
                     /locus_tag="IV328_002936"
     CDS             2858..>3018
                     /locus_tag="IV328_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8938993.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1066 a          339 c          608 g         1005 t
ORIGIN      
        1 agtaatttaa gacgttttca tgaattaccg aaagaaagca gaactaaatg tattgttaca
       61 tcaaatatac aagcatcgga tgtatattta cggacgagtt ataaaaagtt gttatctgaa
      121 agggaagttg taagagacaa tgcaggatta atggcaatta gatttttagc cgatatgaat
      181 gtggatgaaa tattcttagc tggatttgat ggatattcac ataatgaaga tgagaattat
      241 ggtgagcaat ccatggaaat tataacaaaa tttgcattat tagatgcaat gaacgtgcaa
      301 atgagagatg aattgtcaga attagctaaa aaagtaaagg tgacattttt aacggatcca
      361 cgttgggttc gaattgataa gtagtagtaa taatgttttt aaaattttga taaagagata
      421 gtgagagaat aaaaatgggt aaagtcttaa ataaaaaaga agaaaaatat ttaagggacg
      481 atggggcgag atgaaactct aattttgaac ttttaagaat catttctata ttgtgtattg
      541 ttgcttacca tgctaatttg cgtttaaatt ataatgcaga cggaattata gtacatggtt
      601 cattaactgc taaccagatt ttcttgaatt atattggtat ttggggtcag ctaggagtta
      661 attgttttat atttatttcg gcatattttt taagtgagaa aagaaacatt cactttgaaa
      721 atgttttggg aattgtatta gaatgtattt tttatagtgt attagtttat ggaattcttt
      781 gtgcatgtgg aatagaaaca ttttcattga acagctttgt aaaagaaata ttatcatttg
      841 ctctaggtag atattggttt attacatcct atttagcatt gtatattcta agtccgtatt
      901 taaatatcat gcttaacaat ctggaaaaac ttcagttgca aaaaataagt atgttattgt
      961 ttataggtgt atgctgttat cgtatgttat ttaaaacatc tttgtttggg gattgtgctg
     1021 ttttaattgc actatatgtc atatgttata cactgaaaaa aacgataata atatttttga
     1081 gagacacaga atcataggaa caattgtatg tataacagtt ataccacttc tcatatccat
     1141 aggaatactt ttcaaattta taccaaatgc aatggttaac cagatggttc atattggttc
     1201 tccattgatg gttatagcaa gtatattttt attttatgta tttaaaaatt tttcatttta
     1261 cagtaagtat ataaataaac ttgcgagaac cagtttaggg gtatatattt tacatgaaaa
     1321 tcattatttc attgatggta tgaagtctag aggcttaata tgggatggga tatttggatt
     1381 aggcaaaagg tttttagata gggatttttg gatttggttt ataattgcag ttgtagttgt
     1441 gtgcataatt tgcatggcta tcgatgttgt aagagaaaaa tttgaaagac agatttttaa
     1501 aattaagagt gtaaagaacc tgtgtaaact tatgaatcat gttataaata tatacccata
     1561 attaataaat gaggagaaaa gaagtgaaag ttatatcatt cggagaatta ttattgagac
     1621 ttgcagcacc cggttattca aagttatttc aaagagatag tttggagact acattctgcg
     1681 gaggggaagc taatgtagcg gtatccttag caatatttgg atatcaatca gaatttataa
     1741 caaagcttcc ggattcagat gttggatatg cggcagctaa tgctttgcgt tattttggag
     1801 taaatgttga taatattata tttggagagg gacgcatggg actgtattat ttagaaaaag
     1861 gagcagctca gagaccatct aaagtaattt atgacagaaa attttctgca atttcgttag
     1921 ctgatagtga tgaatttaag tggaaggaga tcctaaacga tgcaaattgg tttcattgga
     1981 cagggataaa tccagcattg tctgagaatc tctttgagac gtccttagat gcatgtcagg
     2041 ctgccaaaaa aagaggtata acaatttctt gtgatttaaa ttatcgatcc aagctttgga
     2101 catcagagga agcaaaagaa aagatggaac agatactacc attggttgat atttgtattt
     2161 gtaatgaaga agatgcagaa aaagtgctag gaattaaagc tgagcataca aatattaata
     2221 gtggagaatt aaataaagat ggctatgtgc aggtggctag ggagattcac aagaagtttg
     2281 gatgtaggta tgtagctaca actctgcgta gaagttattc ggcaagtaga aatggttgga
     2341 gtgcaatgtt atacgaagca gaaaaagata aagcgtattt ttccacagaa tacaacatac
     2401 aaattgttga tcgcgttggt ggtggagaca gcttttctgc tggtataata tatgcgttat
     2461 gtgaaaatat gtccggacaa aaggctattg aatttgcgac tgcatctagt tgtttaaaac
     2521 atactataga aggtgatttt aatcgaacta caattgagga tgtatataat ttaatgaata
     2581 gcggtggaaa tggtcgcgtg ttgcgttagt caacatataa agataaatca cagtaatttt
     2641 tatggtgatt atctgtctta gtagtgatta gaataacgtg aaaataaatt gttgagcgtt
     2701 tatactgttt tgaaaaataa atttaacata ttgaaaaata caaatatata cgtatattat
     2761 aaagcgccga ttgtaaaatt aagctagaca actaaaacag tcatttgtgc tacactcaaa
     2821 atagttccga ccaaagaatt ataaggagtg agtgcaaata acctatacct atcttacaac
     2881 ggatgaactt gtaataatag agtcttattt gaaaattaac caatctgttg ctaaaactgc
     2941 gcattgcttg aatcgttcaa gtcaaacgat ccataaagta tatctgttct tcaagcaaag
     3001 aaaatcagcc ttagaata
//
LOCUS       DACDKJ010000143         3012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDKJ010000143 DACDKJ010000000
VERSION     DACDKJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.150"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IV328_002937"
     CDS             177..359
                     /locus_tag="IV328_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP8938994.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IV328_002938"
     CDS             462..746
                     /locus_tag="IV328_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938995.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IV328_002939"
     CDS             762..998
                     /locus_tag="IV328_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938996.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IV328_002940"
     CDS             1020..1274
                     /locus_tag="IV328_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8938997.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IV328_002941"
     CDS             1356..1541
                     /locus_tag="IV328_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8938998.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IV328_002942"
     CDS             1608..2003
                     /locus_tag="IV328_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8938999.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IV328_002943"
     CDS             1993..2370
                     /locus_tag="IV328_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939000.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IV328_002944"
     CDS             2371..2766
                     /locus_tag="IV328_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939001.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DACDKJ010000144         2981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDKJ010000144 DACDKJ010000000
VERSION     DACDKJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.151"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1054
                     /locus_tag="IV328_002945"
     CDS             <1..1054
                     /locus_tag="IV328_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8939002.1"
                     /translation="SYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILK
                     WGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGW
                     WINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETA
                     KFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASG
                     LLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRK
                     RAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINISYIKQLCY
                     RGRNIISMIKSREHKR"
     gene            1147..1806
                     /locus_tag="IV328_002946"
     CDS             1147..1806
                     /locus_tag="IV328_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8939003.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            1825..>2981
                     /locus_tag="IV328_002947"
     CDS             1825..>2981
                     /locus_tag="IV328_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP8939004.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISV"
BASE COUNT         1107 a          352 c          561 g          961 t
ORIGIN      
        1 atcctatgta tggaatcaat ttctaataca atttgctaaa gggttagaaa aagttaaagc
       61 tatgggcata gcaggtatta tcagcacaat tgtaacaata attgccaata ttttcttctt
      121 gcttattctg aaatggggat taattggttt tttcattgca aatattttat cacagattat
      181 ttcagctact tatctgtcag tattagtgag aatatgggaa tatatcgatt ttgaggctaa
      241 agatcctcag ctaaagagag aaatgctaaa ttactcgatt ccattaattg caactgttgt
      301 tggatggtgg attaatagta cagctgatag gtatgtggtt tcctttatag taggtgtctc
      361 ggcgaatggc ttattgtctg tggcatataa aatacctcaa ataattaata ctttgcaggg
      421 aatatttata caggcctggc aaatttctgc aattaaagaa tatggagata atgagactgc
      481 aaaattttat ggaaatacat ttgttatgat taatttgtta atgtgcataa cctgttcaag
      541 tcttatcata ttaactagac cgttttcaca tattctttat gcgaaagact tttatgaagc
      601 gtggaaatat gttccatttt tattaatagc taatgtttta aattgtgcat caggtctgtt
      661 aggacctatc ttatcagcaa aaaaagattc taaatccatg atgtggtctg caatcatagg
      721 ggcattagtt aatgtgatta tgagtgtagt ccttgtttat aagatagggg ttcaaggtgc
      781 cacaatagcc actgttatat gttcatatgt aatatatgtt gttcgtaaac gagctgtggg
      841 aaaagatatt tttataaagc attatcatat tgttgtggaa tcttggggat tattgtttgt
      901 tcaggcaatc atagaaatat attttaaaaa ttatgtaata gaaattataa ttatgattat
      961 tttaattatt ataaatatca gttatattaa gcagttgtgt tatagaggaa gaaacattat
     1021 ttcaatgatt aagagtcgag agcacaaacg gtagacgatg ttatttttat aaaaagatat
     1081 aatttattaa tggtcgataa ttatgaaagg aaaaatattc ttaaaaaaaa gagtatagga
     1141 tgtcgtatga aaatagtcgc tatagtacca atgaagttaa ataacaaaag attgccacaa
     1201 aaaaacacga aagcttttac caatggaaaa ccattgtgtt attatatact ttcaacactt
     1261 ttaaaagtaa aacagattga taaagtgtat gtctattgta gtaacccaga tattaaagaa
     1321 tttataccag aaggtgtaac atttttaaag agatctagtt ccttagatca ggatacaacg
     1381 agtattacac aggtattagc cggtttcaca aaagaagtag aggctgatgt atatgtaatg
     1441 acacacacaa cagcaccatt tattagcgaa aaatctattg aaaaaggact agatgccgtg
     1501 ctgacaggag aatatgattc tgcatttgct gcaaaaaaat tgcaagattt cttatggaaa
     1561 gacggtgaac catttaatta tgaattggac aatattccta gaacacagga tttgccgcct
     1621 ttatacgaag aaactagtgg attttatatt tataatcgca atgtgatgac cgagttgcat
     1681 agaagaattg ggaaaaaacc ttgcatagtt gaagtaggtg agattgagtc agttgatatt
     1741 gatgaagccg aggattttat gattgctgat gcgatttata atcatcttat tttacaaggt
     1801 agttaagaca gggggaaggt taggatgaac aatgtacaga ttttagattg cacacttaga
     1861 gatgggggct attgtaacaa ttgggaattt ggatttgata atacaaaaaa aattatttct
     1921 ggacttatag aatcaggaat tgagattata gaatgtgggt ttctcacaaa taaaacagta
     1981 tacaataaaa atgttacaaa atatacgacc ttagaagaat tagcacagat aattcctgaa
     2041 aaaagagaag gaaaattatt tgtagcaatg atgaactatg gcgaatataa tatagaggat
     2101 cttccagatt atgatggttc atctattgat ggaattagga ttgcgtttca taaaaaaaat
     2161 tgcaatgaag cgttgaaatt atgtcgtgag ataaagaaaa aaggctactt agtttttgtt
     2221 caagctatgg tatctctcaa ttatacagat gaagagtttt tagcagtaat aaaaaatgtg
     2281 aatcagttta aaccatacgc tttttatatt gttgatagtt ttggaatgat gaaaggaaaa
     2341 gatttaactc gtctttttta catgattgag cataatctag aaaaagatat acagataggt
     2401 tttcactcac ataataacat gcagcttgca tattctaatg cacaaaaact tataaccgta
     2461 caaacaaatc gaagcctgat tattgattct tcggtgtacg gaatgggaag aggagcaggt
     2521 aatcttaaca cagaactatt tgtagaatat ttgaatgata atgcaggaaa aaaatatcaa
     2581 ttaaaaccat tattatatat aattgatgaa atactaaata gtttctatca gaagagttat
     2641 tggggatatt cattaccaaa ctatatttca gcagttcata atgctcatcc taattatgca
     2701 agctatctgt catataagaa tactttaaca attgaagcaa tgaatgaaat attcgaaatg
     2761 atggatgaag aaaaaaaggt atcttatgat aaacaatata tagaagattt atactacaaa
     2821 tatatggaaa ctgggaaaat acaggaggaa tatatggaag aactgagaaa atttctctca
     2881 gacaaaaaaa ttctactgat tgcaccagga aaaagttctg tagatgaggt agaaagaata
     2941 aaagagtttg ttaccaaaga agaagtcgta ataattagtg t
//
LOCUS       DACDKJ010000145         2970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDKJ010000145 DACDKJ010000000
VERSION     DACDKJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.152"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..405)
                     /locus_tag="IV328_002948"
                     /pseudo
     CDS             complement(1..405)
                     /locus_tag="IV328_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
     gene            complement(727..948)
                     /locus_tag="IV328_002949"
     CDS             complement(727..948)
                     /locus_tag="IV328_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939005.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1046..1291)
                     /locus_tag="IV328_002950"
     CDS             complement(1046..1291)
                     /locus_tag="IV328_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939006.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1531..2145
                     /locus_tag="IV328_002951"
                     /pseudo
     CDS             1531..2145
                     /locus_tag="IV328_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2515..2892)
                     /locus_tag="IV328_002952"
                     /pseudo
     CDS             complement(2515..2892)
                     /locus_tag="IV328_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
BASE COUNT          988 a          527 c          424 g         1031 t
ORIGIN      
        1 tgctttatcg gtcacaattg ctttcggctc accaaactgt ttatggagtc gttttaagaa
       61 agcataggct gcttgcgtat cccgtttctt tcgtaaccag atatctaagg ttaagccgtc
      121 cgcatcaatt gcacgataaa gataatgcca acgtccctta attttgatat aggtttcgtc
      181 cattttccat gaatagaagg attgtctatt tttcttcttc caaagataat agaggacttt
      241 gctgtactct tatacccaac gataaatcgt agtatgacaa acatttattc cacgatcata
      301 taacaattcc tgaacttcac gatagcttag attgtaacgc aggtagtaac caacagcgac
      361 aataatgacg tcttttttga attgtttgcc tttaaaatga ttcattactc tgtcctctct
      421 gtcttttttc tcaattttac actaaaatag attttttgga aaactttgca acagaaccgt
      481 ttttactata tacttcttcc cagaacttgt ccgtctcctt caaattatct ctacctctcg
      541 agttcattgg gtggtttgcc catttattaa acatggaatc acctttctca tactcatata
      601 tttatgtcct atttcaatta tatttatata tgcttaaata aatttgtatc ctgaaattta
      661 ttgtaatatg aagctgtatt ttacatatag taaccaaaga gttaataaaa taaaaagagc
      721 gaaccattat agatcagctc ttttttcatt gctgttttta atcttatcta gaatcacttc
      781 aaatgtttca aaggtattta catctaactg acataaccaa agcaagtact tcttttcttt
      841 gtaagtccaa tcaatattac ggtaagagga cattagatta tggtaccttg ctctttgttc
      901 aactgaagcc aattcccagc gtttttcaaa tattttttct tcttccatta ttgatccctc
      961 gcaagacgct acctcaaact taaataatgg gcagcgaaag tctaattcct tagatctaag
     1021 tatagcaaac aaatatgaaa aaaacttaga aggtagtaaa agttcttccg tcttttgctt
     1081 tgcttctatt cttaatgtgt ttcgttatcg gtgccctaca aagattttca ttaattacac
     1141 actcatttat tgttgtgtat tttctgacat ttctctcttg atcaattatt ttaattccat
     1201 gggcatcctg atagttcata ggagaggagc ggctatcaga aatagttgca cctatctctt
     1261 gaacttcttt tacaacttct ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata
     1321 atctataata tttatctttc cttttcaatc taagaaatcg tcaccccatt ctttttcttc
     1381 tattataaac tatttaactt ttagaattca aaaaccgttt gtctatttag agtttaccct
     1441 aaatagacaa aaatataaca ttgaaaaatt tagagttcta acgtatgatt agggtgtatt
     1501 ataaacgtta tagaaaagta gggaataaat ttgaaaattg cttatattag agtttcatcc
     1561 atcgatcaaa atgaacaacg tcaaattgaa gaaatgaaaa agtttggcgc tgaaaaaatt
     1621 tttatcgaaa aacaatctgg agcgacgatt agtcatcggc cgattttcca ggaagcttta
     1681 gattttgtca gagagcaaga tatttttatt gtagaagcca ttgatcgact tggacgcaat
     1741 tatgacgaga ttatcgattc agtgaattat ctaaagaaga agaatgtgcg acttattatc
     1801 accagtttac cgatcatggc tgaagcaatt ggaaatcctc tcctagataa attcatcaaa
     1861 gacttaatca tttaaatctc agccatgatt gctgaacaag aacgtactga atcgaaacga
     1921 cgccaggcac aaggaattaa aatcgctaaa gcaaacggcg tctataaagg tcgtcctaag
     1981 ttatacagtg cagacaccaa agatcctcaa cgtcgccttg tctataggag tatcgttcag
     2041 gatcttgaga atggggttgc tatctcaaag attgcaacag actacaatgt gactaggcaa
     2101 acaatttacc gaataaaaaa agaaatcgac caactaattg tatgatttct tttttgaatc
     2161 acgtaattat taattagctt tgtcatcaat acagacaatc attttagatt catctagcta
     2221 agttgattac atttatgtta aaaagaattt agttaacata aatataattg atgcataaat
     2281 cgtccttatt taaaggcatt taacattgac aacttagtat aatgtatctt atgtaaacta
     2341 ataagatttg aattttattt tatacacact cgataataga tcaattttat ggtcccccgc
     2401 atttacgatt gcacaagaga aacataaagt cgacctatta gcatttcttt tcaattcaaa
     2461 cattaaaaaa gtttatacct ctgctactaa aaaatgaaat tatctattta aaatttaaaa
     2521 cggtaaatct gattcatcca aattggaaaa tggatcagcc tgtattggag ctttgtattt
     2581 ctccataata acagatatct ttttttcatt ttttaaaaaa taccttttaa atttcttata
     2641 gtcctctgaa atcaaaatat agcgtgatgg atactcatca aaagaagaat cgtattcttc
     2701 ctttaaaatt cccgatttac ttaatttttc tagtacatca aatactctat cactcaagta
     2761 actccttaaa ttgttatcac tttcccattg aactactttt ctataagttg aattatcagt
     2821 tgaaaagata gaattttcat ccataaacgc atatattagc agcaatcttt catcattgtc
     2881 aaattctatt ggatgaatcg gctacactag actagacaga aaaaataagg tgtgtagaat
     2941 aataaaaaac acactggagg atttttcatg
//
LOCUS       DACDKJ010000146         2893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDKJ010000146 DACDKJ010000000
VERSION     DACDKJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.153"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(260..544)
                     /locus_tag="IV328_002953"
     CDS             complement(260..544)
                     /locus_tag="IV328_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939007.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(565..1935)
                     /locus_tag="IV328_002954"
     CDS             complement(565..1935)
                     /locus_tag="IV328_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP8939008.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            2154..>2893
                     /locus_tag="IV328_002955"
     CDS             2154..>2893
                     /locus_tag="IV328_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8939009.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTV"
BASE COUNT          912 a          590 c          439 g          952 t
ORIGIN      
        1 atgtgaaaat cgaatcttga gatagctatt ccccttgaga tattcactga tacttctact
       61 caaatcattt aaatttttat aattatttgt acggaacgta tatatatcaa catctattta
      121 gacgttgtcg gtgatacaat gtcatttttc ccgtgtacta tgtcatttat ctctatactt
      181 tgtcattttt ctttttgggc aatcaaaaaa ggcaacaaac caattatgat ttgctacctt
      241 ctccgtttaa aaacttatct tagccccaaa atggaatcca atttttaact tcttttgcca
      301 atgcatacgc cttagcaaga cctgagtttt caactaaata tttttcccct tccagagaaa
      361 cactccccca gatatgaaca gtgttatccg cccaaaccat ggattctagt aagccattgc
      421 gcgaaagaaa tttagtttgt tctaaaatat cttcttctgg aacttgccct ttaagctcaa
      481 taaaaggatt ctttttttca tcacgcaatt taagaattgc atatcttagt ttgttcttat
      541 ccaacctaac accacctcta tgctctattt cttatttctt agcgaatcat taatacttat
      601 tccgttatca tatttccagt agtcccaacc attccggcta ccccctaata gcagagctgc
      661 tgcattcgaa acagatgagg cagtgtaatc ttgaacaaat tgtagtcccc caattgtttc
      721 ttcaatgatt ccattatcca acagctcttg gcgagtttca tctagcgaat cagatgcaat
      781 atctttaatc ttagaataaa tttctgaacc tgaatgtata atcacggtct cgtcttcgtt
      841 caaataccca gttgctttta ttccttgcgc ctctaagaaa atttccctat tcccatcgtg
      901 ttcatagtcg cccgacgatc tcaaataggg atacatagta atcagttcat tgattagagc
      961 ttcacttctg gcagaaatat ccgatgatgt ccactcagtc aattcgtaca aatctttatt
     1021 catacgaata tgaagggtat cttcaaagat tttctttttg gtctcaaagt ctttatttcc
     1081 cgcggccgaa ttatcaggat ttgtaacgag tgttaaatta ccaatggtat ttactaatcc
     1141 tgtgtattct tcccccgatg taccagcctt ctcttcccaa tatgaagtac tagtctgtgg
     1201 catgatgtgt tcaatggaca agttactcat atcaagtgtt gctgaatttt ctcgattctc
     1261 tattttttca agtaaccaac gggccaatct catcgcataa gcattggcat tcaatagatt
     1321 agatttcaat gccttgtcag atggaagtgc catattattt gattgatttc tagtaacaag
     1381 aacataaatt acaatatcaa cgatattttc atatccatta gctttagcgt acttcctaac
     1441 acttttcaaa tatgaaggaa atgcttttga cactctgctt gtatcatccc cattaaaata
     1501 tctcctaagc tgataatcat tcactacctt aatcgcatca atatattggt cttcagtgat
     1561 aaattggtca tgatagtacc attcagataa tcccaaaaca aaaggtgcag gcatcatact
     1621 ttccatactt tggaaatcgc tcaatacttc tgagtatttt ccttcaggtt tgttatagta
     1681 aagtctcgca aaataaccag catatcgcac taatttttgg agcaattcgg catcacttga
     1741 tacatcgcgc tctttatgcc aatagtcttt gaatgcttga taaagaatat gctttgctga
     1801 atactcccca gttattgcag caaggtagta tctgaaaaag tcttccattc ctttagaatt
     1861 atcaaagact tcttccaacc tacgccaata tttagtataa agcgtagtct gctcttcatt
     1921 agatttatcc atcatgatga agtttcgaat caaatccgag gcagtcaatc tttcaccggt
     1981 agaattgata ctttcaaaaa tttgctgtgc attatcacgg tcatctaatt taatataaac
     2041 aatacgcgtg gtgctagtta attttttcct agaggaaaat gtctaaacat gttggtaaat
     2101 caacaaaaaa cgcatatagt agtctcaaaa ctcccaagaa atgaggacta catatgcgtt
     2161 catcaaaata tactgaacat tatacagata cttttataac attcaaggaa attatgagaa
     2221 ctgtttctaa catataccac aattgtgtac cagataagat taaaaaccga agaaatactg
     2281 atcaactgaa gcaacgcgat acagtgatta ttgcttgtgt tatatggggc ataattaatg
     2341 gctatactag ccaaagagcc acgtatagag cggtttgttc cgtcttattt cttaatggtg
     2401 actttcctag taggagtcga ttcactcgct taagttcaaa cttagcttac actatcaaga
     2461 ttattcgata ctttttcatc aagaagctca ccaaaggtga actagtcgga attatagata
     2521 gttttcctag tcaattatgt aaaccagtta gaaatagaca agcaaaacta ttgaatcaaa
     2581 tagccaaagt tggctataac tcaacaaaaa aatcttattt ttatggtctt aaaattcata
     2641 tgatcgttac taagactggc tttccgatta cttactcaat cacgaatcca ggtgtccatg
     2701 acgtgaaagt gttagagact ttatcggaag aagcgaatct tcccaatatc ctaggggaca
     2761 aaggatatat tagccataaa atccatgaaa agcttgccct taagggcatt actatatccg
     2821 ttccgccacg taaaaacatg gataaatcag aaaaactaga ccacagctta cttggaaaac
     2881 aaagaaaaac agt
//
LOCUS       DACDKJ010000147         2830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDKJ010000147 DACDKJ010000000
VERSION     DACDKJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.154"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            802..1068
                     /locus_tag="IV328_002956"
     CDS             802..1068
                     /locus_tag="IV328_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939010.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1126..1638
                     /locus_tag="IV328_002957"
     CDS             1126..1638
                     /locus_tag="IV328_002957"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8939011.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1635..2672
                     /locus_tag="IV328_002958"
     CDS             1635..2672
                     /locus_tag="IV328_002958"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8939012.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1023 a          496 c          564 g          747 t
ORIGIN      
        1 tttattgatg accacgaaag tgcatggcga tatttgatgt tgatacttta tgcagtaccc
       61 tatgtattct tcgctatcat tctgtggctc atagcattgt tttttagagt atttaatgac
      121 ctttaggtag atttatatat aaaaagggct aaatacgcaa aataccccct taaaatgcaa
      181 aataaatgca aataaggggg taactcctta tgaaaaaaca actaatagta ttataacacg
      241 tttttatgcc gagaaaactt attggttgga atgggctatg tgttagctaa cttgttagcg
      301 agttggttgg acttgaattg ggattaatcc caagaaagta ccaactcaac aacacataaa
      361 gccctgtagg ttccgaccaa taaggaaatt ggaataaagc aacgaaagga gttgagtaaa
      421 atgagtgaga aaacgaagaa aatcattcga gtatcattag ctttttcaca agtagtagtg
      481 caatcattga tagtttatta tacttaccag caatataaat tatctaaaga gaattaagtt
      541 ttgattatgt aataagtttt ggtactaaaa aagacttgat ctaattagac caagtctttt
      601 gttagtgtta tattaataac aaaataaaaa taagaagtcg ctcactcccc gaccaaagtt
      661 tgtgagcaaa ataaacagtc gatgtttgtt tcttaattgt atatcttgat attaaacgat
      721 ttaagattat tcttcaagat atatattcgc aaatgtaatt ggaaaagcga cttcttaaac
      781 aaaattaagg agtcgctttt tatggcaaaa ataacgaaaa atgagcaaga aaatttggtg
      841 gaaaattaca caaaaaaacc ggcgtactct aatagccggt tagacgcaca tttcgtgtgc
      901 acttctaatc cgaaattgag ctttgacgct atgacgatag tcggaaacct taataaaaac
      961 agcgctaaaa agctatccga ttttatgagt ttagatccac aaattagact ttgggatata
     1021 cttcaaacaa aatttaaagc taaagcacta caagaccccc tatgttaggc tagttgtcgt
     1081 ataggataat aagttataat aaagactata cggaggatga ttaccatgac aaataaaaac
     1141 aatgaacgtt actctaccga actgaaagcg acagccatca agaaaatgat gccacccgaa
     1201 aataggggga ttcgccaatt aagtcaaact ttgggcattc cagaagcgac tctttattct
     1261 tggcgtaaaa aagctagaat tcaagggaat actacacccg gaacgaaaaa aaatgtcgaa
     1321 aaatggtcaa gtgaagataa atttttaatt gtgatggaat cattctcaat gaaccaagta
     1381 gaacttgcgg aatattgccg aaagaagggc ttatttaaag aacaaattga ggcttggaag
     1441 aaaacgtgct tatctgcgaa tgaacaggaa gaaaatcgaa cgagagaact tgcaactgaa
     1501 ctaaaagaag agaaaaaaca agcgcgtcaa ttagagaaag aattacgtca taaggaaaaa
     1561 gcattagcgg aagccgctgc tctcttatta ctaagaaaaa aagcacacgc gatttggggg
     1621 gaccaagagg aagaatgatt tcatcttcag atcgcaaaca agctgttgaa cttattcaag
     1681 aagccaatcg caatggggcc cgtctcacct atgcctgtaa tgagctgaat attagcgttc
     1741 gaacctacga acgatggacg cgtgaaggta cgatagcgca tgatcaacgc cctttggcga
     1801 aacgtcctgt acccaaaaat aaactgacgg atcaagaacg cgaaaaaata atagaaaccg
     1861 tttcaaaaaa agaatttatg aatttgccac ctagccaaat tgtaccaaaa cttgcggatt
     1921 gctcgattta tattgcatca gagtctagct tctatcgtgt attgcgagaa aaaaatatgc
     1981 aacatcaccg tggccgaagt caagtttcac aaaaacgcat accgccaagt caccttgcga
     2041 cgaagccaaa tgaagtttgg acttgggata ttacttggct aaaaggtcca ataaaaggac
     2101 tcttttaccg gttatatctc atcattgatt tatttagccg taaaatggta ggctgggaga
     2161 tttgggaatc agaagaagct cgtcatgccg aaaaacttat taaacagacg gtgcttaagg
     2221 aacgtatcca aggacagccg ttggttttac actccgataa tggcagccct atgaaagccg
     2281 cgacttttca agtcttactc gaaaaattag gaattcaaag ttcttattca agaccacgag
     2341 tgagtaatga caatccttat tcggaagcgg ctttccgaac gttaaaatat cgaccagaat
     2401 atcccaataa cggctttaaa acaatagatg atgctcgaaa atggacgaaa gagtttgtcg
     2461 attggtataa cgattctcat ttgcatagtg ggattcaatt tgtgtctcct gcagcgtgtc
     2521 atgaaggacg acatctcgct attttagaga aacgaaagca ggtttacgag gacgctaaaa
     2581 agcgccatcc tgaacgctgg tcaaaaggtg ttcgaaattg gactccacat caatcggtgg
     2641 cgttgaatcc tttaaaagaa acagaaagtt aacaggagca taatctttca agattatcaa
     2701 ataaccctcg tcacccaata ggggaaaaag ttattcttcc cctattggat aacgaggcca
     2761 accgaccata gggaggttct gctttttgat aggtggaaaa taaaaatacg acaactatat
     2821 tgacaaacac
//
LOCUS       DACDKJ010000148         2548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDKJ010000148 DACDKJ010000000
VERSION     DACDKJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.155"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IV328_002959"
     CDS             <1..1350
                     /locus_tag="IV328_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8939013.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2548
                     /locus_tag="IV328_002960"
     CDS             1484..>2548
                     /locus_tag="IV328_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8939014.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESID"
BASE COUNT          998 a          356 c          507 g          687 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagat
//
LOCUS       DACDKJ010000149         2407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDKJ010000149 DACDKJ010000000
VERSION     DACDKJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.156"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IV328_002961"
     CDS             5..1150
                     /locus_tag="IV328_002961"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8939015.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IV328_002962"
     CDS             1321..1779
                     /locus_tag="IV328_002962"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8939016.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="IV328_002963"
     CDS             1938..>2407
                     /locus_tag="IV328_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8939017.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DACDKJ010000150         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDKJ010000150 DACDKJ010000000
VERSION     DACDKJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.157"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IV328_002964"
     CDS             207..2069
                     /locus_tag="IV328_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8939018.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IV328_002965"
     CDS             2069..>2398
                     /locus_tag="IV328_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8939019.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACDKJ010000151         2339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDKJ010000151 DACDKJ010000000
VERSION     DACDKJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.158"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            403..1221
                     /locus_tag="IV328_002966"
     CDS             403..1221
                     /locus_tag="IV328_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8939020.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1294..1653)
                     /locus_tag="IV328_002967"
     CDS             complement(1294..1653)
                     /locus_tag="IV328_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8939021.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          800 a          340 c          406 g          793 t
ORIGIN      
        1 tttattacct gattgaacat gaataaaagt aatattttta ttttgatggc ctatttctag
       61 gttatgaatt atccagatca aatttgtgta ttacattcag taataaggtg cttatcttag
      121 gatcaactta tgatactagc tgaccaagga tgtaccagtt atagaaaatc agttaaagta
      181 aaaatgataa aaatcttatg tcaacactga tttaggctga cctgtgggtt aggagaaaga
      241 caggtcggta gcttttaata caaaaaatgg cttgtatgta tctagatagt taactgaaat
      301 tagaaatgtt cttattttta acggagtaag aaaaataggc tgctttaggt ctgaatactt
      361 tttcaatata atgaagatat caaaggcgac aggagtgttt ttatgaataa gaatcctcat
      421 aatctaaagt tggtagcagt agatatggat ggaacatttg ctcgtactga ttacacatac
      481 gatatcccaa ggttcaaggc tgttttatcg cgaatgaaag ctgttgattg tcaatttgtt
      541 gttgcaagtg gaaatcaata ctaccaattg agatcgttat ttccaaatta ttatgatgaa
      601 ttaagttttg ttgctgaaaa tggtgctttt gttaaagata aaacagaggt actattttca
      661 gccgatatgc ctagaaaagc gatcgaggtg gtccttgatt tatgtaaaga ctatcctgaa
      721 gtaaaaaatg tgatgtgtgg tctgaatagt gcctactgtg aaagaggaaa ggtgagtcaa
      781 gcattttttg atctaaccaa tatctattac catagattag aatgggtagc tgattttgca
      841 gaagttcaag ataaaatttt aaaatttgct ccgactatgc ctgttgaaaa aaccgaattt
      901 tacgttgaat tgttccgtga aaaattagcc gggctattag tacctaccag tagtggtcat
      961 ggttctgtag acttgattct ttctgagtgc cataaaactt tcggcttgaa aatgttagtt
     1021 gagcgcttgg ggattaatcc tgaccagtgc gttgcttttg gagatggggg caatgacatt
     1081 gagatgttgg agtattgtgg tctgagttat gaaatggata atgcaacgga agctgttaag
     1141 caagttgcta agcatcaatg tccttcaaat gacgaagacg gtgttttagt gacgctcgat
     1201 cgattgttcc ctaacacgta aacactaggt aattcatcaa aaaacctcga tataagaaat
     1261 attcgaggtt ttaaaactat atttagaaca gatttacgaa gcgtctgatg agtcaaaata
     1321 gacttggaca ttgctatcta aaataccact ttcattcttt aaacgaattg tttgatttga
     1381 tggagttacc tctaatacta aatgcccatt aataaaggta gtatcgtatt cagatgtttt
     1441 aactgttgct tcttcgcaag gtattgttaa atcatatgct ggaatataat gttgtgtgga
     1501 aactaataaa aaatgatcac caatcataca gcatccctct gctgaagaaa attgattaga
     1561 gccatcattt aaagttaaca agaatccatc ctttaaacga ggtacttgct taaatacttc
     1621 tggttgtact tctaaaaata aattatcgct cattttcacg accaccatcg acttccacag
     1681 ttgaaattga cattttattt aacacaccta ttccccctta actataactc aattctatat
     1741 aaaaaatatt ctattctcaa ataattgaac ttttcaaatc gccaatttac ttcggcttta
     1801 tcagagctca ttcatatcaa gagcgattaa cgctgtaaaa attaaaccga agatctgata
     1861 aattttctct attttctaca atatttaaaa cgcttcctta gtctattata tatctatttc
     1921 tcttaggagt atgtttagat gtttgaataa tagttgccca actttaaagg ttattcttat
     1981 aatattttac ttcattttcc aatagaaaat tcaaaggatt actggttttt ctaaaatagt
     2041 ttagtttaaa tacataagta tcctccgaca ctgtattagt tcataatcat ctaaatacaa
     2101 aattttcaaa taaaaatatt tcaagctcta tcgttgacga atgtaaacga tacttataaa
     2161 gtaatagaaa ttgattacat ttgtaaactt aaaatggtta atttatctca cataaaacta
     2221 agaataaaat aatgattaaa agtggttagt ttttatacga tttagcctat cggtaaaaat
     2281 gaattagaga gtgtaaaata ttttgtgtaa atgaaaaaat ccatacaaaa aaggaagtc
//
LOCUS       DACDKJ010000152         2323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDKJ010000152 DACDKJ010000000
VERSION     DACDKJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.159"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..586)
                     /locus_tag="IV328_002968"
     CDS             complement(<1..586)
                     /locus_tag="IV328_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939022.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGF"
     gene            complement(588..1256)
                     /locus_tag="IV328_002969"
     CDS             complement(588..1256)
                     /locus_tag="IV328_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8939023.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1386..1970)
                     /locus_tag="IV328_002970"
     CDS             complement(1386..1970)
                     /locus_tag="IV328_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8939024.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          827 a          395 c          301 g          800 t
ORIGIN      
        1 taaatccttt attttttaaa tcacaaagta agtcagcaag tttcttagtc gctttgaagt
       61 ccaaatttga agacggttca tccaatataa ttaatttaga gtttagcgat aaactacatc
      121 caatagaaat tttctgccgt tcaccacttg atagcgaaaa aatgttttta tctaatatat
      181 ttgaaagatc taataattca gctaattcat tgattctttt atccatatgc tcttttgtaa
      241 atccaaagtt ttccatcgaa aatactaact cactcgttgt atttgttgta aagaattgtc
      301 ctctaggatc ttgacttacg tagccaatat tttttgatat actttcttgt gttaaggatt
      361 gtagcgagtt attttcaaat aaaatttccc caaccaattt tccgtcataa agtcttggaa
      421 taagtccatt cattaacttt aatagtgtgg atttcccact cccagaagaa ccagtaatca
      481 atgtaagact gccttttttt attgaaaaat taaggtcaac aagcgcttgt tccttaacat
      541 ccttatatga aaaagaaaca ttgttaaatt caaacaattc tttcacctca aatcaaaata
      601 cttaagaaaa aaatagtaac ggaaaacagg atgatatagt ctaataagct catgtttgtt
      661 ttatagtaag aacttctttt cacaggagat tctatcccct ttgttattgc attagcggtc
      721 atttcttcag caatcatagt tgcacgtata agaacgggaa ctaacagaaa ttccaaatat
      781 tgcagaggct gaaaaaaaga caagcgaatg tttctcattt ttaaagattc tttaatcatt
      841 cttatttcag aacgataagt cggaaagaat ctgatcacta ctgtaatact taacattatt
      901 tttttcggta aatgtatttt ttctaaagag gataataatt cattgctagt atagacacta
      961 accaacaacc atgagatcat tacgaccggc aacattcgaa atacaatata taaggatgtc
     1021 gcaaaaaata tagtaaaaaa ctctatcttg atagcatcaa taattttcat tactaaaaac
     1081 agaactgaat aaatcaagat gaaataaata catgtcttta ttttacgaaa catcaatact
     1141 aaaactgtag caataatgaa acaccccact tgaaatatat ttccctttcc taaaaaaata
     1201 atgaccggaa aaaaaatatt taaagtaatc aaggttaacg gattgatttt tgacactctt
     1261 tcacctccaa aaaataagat cgcactgtta tcttaagctg ataaaaatgc accgttattc
     1321 agataacagt gcgatcttat caaaaagcat aactgagaac aattctcaat gcaagctttt
     1381 tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa tcttctggtt
     1441 aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa tgtagcgtag
     1501 aatgagtaaa tgtagaagtg gatttcttct tcattaaaga ctaaattttg attgatcatt
     1561 ccgatagtct ttaacttatc tagacaacag actgtctttt tcgtcacaaa tgatgtaatt
     1621 ttttcaacaa tatcttgata tttggttcca gctgattgga ataaaatcaa atcgaatata
     1681 cgtttattct caaaaatata attaataaat ggatctaatt cattgaagtt ctctctccaa
     1741 cttttctcta tatctatatt ttctaagttt aagaggtatt tggtaaactc ttcattatac
     1801 atattaataa gcgcattaat atgcggctga ataatttctt caaataacgc ttccttagaa
     1861 gtaaaatgtt tataaaaagc acccgtagtt acgcctgcgg tcaggcacag ttgccttatc
     1921 ttagtttgct gaaatccttc tttttcaaaa tggcttagtc cactttctat taacttttgg
     1981 tgagttataa tataatctct ggtttctatg ggtacttcct cctaataaga tgaattaact
     2041 ttattatctt acacaggact tgaagtttca atcgttaacg tttattactg aactcattcc
     2101 ttagagctaa actaatgaga aagagcagaa taactgatac aaaaagcaat cctacaagaa
     2161 ttaatagact catctaaatc attcaccttt tctaaaataa tccgagaaag atttaaaaaa
     2221 attatatcca gctattagga taagtcctat cataatcact ccaatagcca ttttcttatc
     2281 ctccctaaaa ataaagtctt ttacttaaaa ccttttttgt aat
//
LOCUS       DACDKJ010000153         2280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDKJ010000153 DACDKJ010000000
VERSION     DACDKJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.160"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IV328_002971"
     CDS             132..344
                     /locus_tag="IV328_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939025.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IV328_002972"
     CDS             506..1024
                     /locus_tag="IV328_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP8939026.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IV328_002973"
     CDS             1031..1543
                     /locus_tag="IV328_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939027.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..>2280
                     /locus_tag="IV328_002974"
     CDS             1814..>2280
                     /locus_tag="IV328_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8939028.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGK"
BASE COUNT          866 a          289 c          375 g          750 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
//
LOCUS       DACDKJ010000154         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDKJ010000154 DACDKJ010000000
VERSION     DACDKJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.161"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IV328_002975"
     CDS             <1..544
                     /locus_tag="IV328_002975"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8939029.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="IV328_002976"
     CDS             complement(900..1472)
                     /locus_tag="IV328_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8939030.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="IV328_002977"
     CDS             complement(1488..2093)
                     /locus_tag="IV328_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8939031.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DACDKJ010000155         2203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDKJ010000155 DACDKJ010000000
VERSION     DACDKJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.162"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..916
                     /locus_tag="IV328_002978"
     CDS             <1..916
                     /locus_tag="IV328_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939032.1"
                     /translation="FNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQG
                     ENYFTKILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKK
                     KIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPL
                     LSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLEN
                     DFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCE
                     HYNRLSEEKILLPENWAELRKLVEYGII"
     gene            903..>2203
                     /locus_tag="IV328_002979"
     CDS             903..>2203
                     /locus_tag="IV328_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8939033.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSL"
BASE COUNT          754 a          277 c          333 g          839 t
ORIGIN      
        1 atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta aacgtaaaat
       61 taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt atgatgaaat
      121 ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt acaaaaaaaa
      181 tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga agaagagtat
      241 attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa atagcatctt
      301 caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt atgatgtaaa
      361 aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa acatacaatc
      421 aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg atcaaccttt
      481 attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa atagtatatt
      541 ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa atcgttatgg
      601 tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg aaaatgactt
      661 ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat ctatgatgtt
      721 tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat atacagcatt
      781 atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac attataaccg
      841 tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa agttggtaga
      901 gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt ttctggtata
      961 ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt tttgtaaata
     1021 ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta tcaatttttt
     1081 cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt gactataaag
     1141 atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg ttaattgtgt
     1201 tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat atgaaccaac
     1261 ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt tatatccagt
     1321 cttattttac gattaacttt aaccatttta aatatttgat tttatctttt atatctactt
     1381 ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat gataaatatg
     1441 ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta atttatttaa
     1501 gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac gctttaaaaa
     1561 tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa ttcgatagaa
     1621 ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac attagtaaca
     1681 taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt ccatggttct
     1741 atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac tattatttag
     1801 tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta gcaaaattta
     1861 tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc ttaggctatt
     1921 tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa aaaacatcgt
     1981 ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac atgattttca
     2041 tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat tttttacttt
     2101 ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt gacacaaaaa
     2161 aaatatgtat cataataatt tttgtatcaa ttttttcttt gat
//
LOCUS       DACDKJ010000156         2186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDKJ010000156 DACDKJ010000000
VERSION     DACDKJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.163"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..745
                     /locus_tag="IV328_002980"
     CDS             <1..745
                     /locus_tag="IV328_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939034.1"
                     /translation="FAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMG
                     LKRIELLALSGAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMS
                     NLGITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASL
                     HSDILTIYIEVGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDN
                     TLTYPDTQMIFLLVPMIVSWVDNQQRISE"
     gene            829..1917
                     /locus_tag="IV328_002981"
     CDS             829..1917
                     /locus_tag="IV328_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939035.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            1914..>2186
                     /locus_tag="IV328_002982"
     CDS             1914..>2186
                     /locus_tag="IV328_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8939036.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLS"
BASE COUNT          803 a          237 c          376 g          770 t
ORIGIN      
        1 ttttgctatg gaggtacatg atttaacttt tgcgatgggg gtttttttcc tttattacgt
       61 attttttgaa aataaaaata caaaaaaaca taagataaaa attatcgtat ctttaatttt
      121 aattttcatg ggattgaaga gaattgaact gttagcttta agtggtgcaa ttctagtgta
      181 ttatgtttta ttaaagtggg gaaaaaagat tcaaatccgt gcattagtgt gtactatttg
      241 tgcaactgtt atttcgttag tgtttattta cattatagat acaggaattt tggcattgct
      301 tatgtcaaat ttgggcatta cagatgacgg tgcgaggctt tcattttatc tatattcatc
      361 taaattttat gaacttggtt taggatatgt aggaacagga tggacatggt tttcaaaata
      421 cttttttaat ctttatttat cgcactttag gttaaatggt ggaagaattg cagcaagcct
      481 acacagtgat atattaacta tttatataga agttggatgt atagttttta ttatatggat
      541 gttttggtta ttttttgtga aaccgataag actaactaaa aaatttggat tgaaaacagg
      601 ggagtgtgta ttattattaa ctatatatat gtttatatta tatttgacgg ataatacgct
      661 cacttatcca gacactcaaa tgatttttct cttagtacct atgatagttt cgtgggttga
      721 taatcaacaa cgaatatcgg aatagatagc taaagtatat tttaattatg aatttataat
      781 tagttgtaaa agttaatatt taacggacag gaagtagggt gaaaaaattt gaaaaaaatt
      841 ttagtgttag caaatacata ttatcaaata atagtagcaa tacaaatgaa aaatacaatt
      901 ttttccgatg agtatgttga aattattatt tctgatcatt ctaaaaacac taaagaaatt
      961 gcgaaaaaaa tagaagaaaa aaaagtgttt gatggctgtc attatttaga aagcaaatct
     1021 tttgatgata atattgggta ttttcaaaag attaaggact gtcttcagat ttcttgtggt
     1081 aaatcaaatc gatatgccta ttatttgaaa aactgtaaaa gtgaatattt tgatgaattt
     1141 atttgttttt caatgggaga ggtttggttg gaaggaatat atgcttgctt atatcgaaat
     1201 aataaaaatg tacaggtatc aattttagaa gaaggtattt taagctatgg agttgacatt
     1261 attccaacag caagaacaaa agctatcgca gtactcaatt acttaaaaaa aaggactcca
     1321 ctatataaca aaagctataa tttttattgt ttttttccta atatatatac aggaagagag
     1381 aatacaatac aaattccatt agttacagct gggtcaagaa ccgcattgca actttcggat
     1441 atttttaacc ctatgataga tatttataaa gaaaaaaaat atatattttt tacgtctgtt
     1501 tatgattttg agggcggaga acctgtcgga gaatatgaat tagtatgcaa aatagcagac
     1561 ttagtaggaa aagataatct aattgttaag acgcatccta gagatgtaag aacaatttat
     1621 aaagataatg gatttatcga ggataaaaat tcatcaatac cttgggaggc gattcaattg
     1681 tcaggcgatt ttaacgataa aatatttatg acaattaatt caggaagtgt tatttcaggt
     1741 agtacaatgt ctgcaaaacc tgtacgcacg tattatatgt ataaattgtg taacatagaa
     1801 ggaaatccgt cttgcaaaaa gaatgcgtat gatattgagc gattgcttaa gcaaaaaacg
     1861 atgaaaaaca ttttaaagaa tgtgaaaata gcagaaagct tagatgatat actatgagca
     1921 aaactaaata tttaattaaa aatatgggaa tattaacaat aagtaatttt tcttcaaaaa
     1981 ttttagtttt tttgttagtc cctttatata caagtgtatt atccacaaca gaatatggca
     2041 catatgattt gacaatttca acagccacat tattgtttcc aattttaacc ttaaacattg
     2101 tagatgctgt gatgcgcttt ttaatggata aagataaaaa taaaaaaaat atagcatcta
     2161 tagcgattaa agttatattt ttaagc
//
LOCUS       DACDKJ010000157         2062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDKJ010000157 DACDKJ010000000
VERSION     DACDKJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.164"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IV328_002983"
     CDS             <1..378
                     /locus_tag="IV328_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8939037.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IV328_002984"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IV328_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8939038.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="IV328_002985"
     CDS             1859..2026
                     /locus_tag="IV328_002985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939039.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
BASE COUNT          580 a          345 c          442 g          695 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa ga
//
LOCUS       DACDKJ010000158         2058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDKJ010000158 DACDKJ010000000
VERSION     DACDKJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.165"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..337)
                     /locus_tag="IV328_002986"
     CDS             complement(<1..337)
                     /locus_tag="IV328_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8939040.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="IV328_002987"
     CDS             complement(404..559)
                     /locus_tag="IV328_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8939041.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="IV328_002988"
     CDS             1176..1397
                     /locus_tag="IV328_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939042.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="IV328_002989"
     CDS             1398..1655
                     /locus_tag="IV328_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939043.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="IV328_002990"
     CDS             complement(1791..>2058)
                     /locus_tag="IV328_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8939044.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DACDKJ010000159         2041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDKJ010000159 DACDKJ010000000
VERSION     DACDKJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.166"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..249
                     /locus_tag="IV328_002991"
     CDS             <1..249
                     /locus_tag="IV328_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8939045.1"
                     /translation="LDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESA
                     NDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            250..1002
                     /locus_tag="IV328_002992"
     CDS             250..1002
                     /locus_tag="IV328_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8939046.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1007..1954
                     /locus_tag="IV328_002993"
     CDS             1007..1954
                     /locus_tag="IV328_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8939047.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          715 a          270 c          402 g          654 t
ORIGIN      
        1 ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt agatgaatgt
       61 atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt taaagaaatg
      121 cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga aaacgtagta
      181 acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa aatagctgag
      241 gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa ggggtttaaa
      301 aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt agcaatgata
      361 gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa tacggacagt
      421 aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt agaacgtaaa
      481 cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc attcttacaa
      541 ctagaaagag aagataatat atttgattta attatagatt tagatttgac ttcaccttta
      601 cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt agataaagac
      661 gtctactttt cagttgtttc tgcaagacgt aatccctatt ttaatatggt aaaaagaatt
      721 gaaaatgaag ttactatagt taataaatca agatacacat ctaggcaaca gacacctgaa
      781 gtttttgaaa tgaatgcctc aatgtattct tttaaacctg aatttctaaa aaaaagtaat
      841 tatatttttg atgggcaatg tgggataata aaaatgagag attatctaat tttagatatt
      901 gatagtgaag aagattttaa atggttagaa tttttgtttc ctaaattttt agaagattct
      961 tcggagcttc aagatatcta taattattta tatcggaggt aaaaggatga gctgttatct
     1021 tatcaccggt ggggctggct tcattggttc taccttagcc aatcatttag gagaagaaca
     1081 tgtggtaatc gtcgtcgatg acttatcaat gggaaaagta gaaaatcttg atatgacgaa
     1141 aaatatcact tttattgagg gagatgtcgc tgagaaatcc ttgatggaag agatcatgaa
     1201 agctaataaa tttgattata tctttcattt agcagcagtt gctagtgtgg cagattcagt
     1261 tgcccgacca gtagaaacac atcgtgtgaa ttttgaaagt gtattaatga ttttagaatt
     1321 gatccgcaag tatcaacctg atttaaaacg gatcgtgttt agttcttcag cggctgttta
     1381 tggggatgaa ccgactttac caaaaaaaga agagtccgtg attcgcccat tgacgcctta
     1441 tgcggttgat aaattcgctg cagaacaata tgtgctagat tattgtcatt tatatgatgt
     1501 acccggtagc gcagttcgtt ttttcaatgt ctatggtcca aatcaaaatc cgaattctcc
     1561 atacagtggt gtgatttcta ttctcgtaga tcgctataag aaacaaatcg taggagaagc
     1621 aacttcattt acattatttg gtgatggaag ccagtctaga gactttgttt atatagatga
     1681 tgtaatccaa gcattacttt tagttgcgaa tgaggaagct gcactgggga aacaattcaa
     1741 tgttggaacg ggtaaagcaa ccacactttt ggaattgatt catgcaatca atgaaattct
     1801 cggtgtagaa ttgacacttg aatacaaaga agagcgttcg ggtgatatcc atgactctct
     1861 agcagatatt actaagatcc agtcaattgg ctatcaaccg aaatatggta ttttaagtgg
     1921 aatgactaat tatttgaaaa ctgaaatcaa ttaaaacata ctaagataag attagagggt
     1981 aaattcaggc tctttgtcaa taaggactga tgagttttgt aaaatcaaat ctgggataga
     2041 a
//
LOCUS       DACDKJ010000160         1932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDKJ010000160 DACDKJ010000000
VERSION     DACDKJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.167"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..990
                     /locus_tag="IV328_002994"
     CDS             <1..990
                     /locus_tag="IV328_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8939048.1"
                     /translation="LTALFLLPINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGI
                     DAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIEL
                     QRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNH
                     TDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHA
                     HIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNT
                     SGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1180..1253
                     /locus_tag="IV328_002995"
     tRNA            1180..1253
                     /locus_tag="IV328_002995"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1214..1216,aa:Ile,seq:cat)
BASE COUNT          643 a          330 c          364 g          595 t
ORIGIN      
        1 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
       61 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
      121 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
      181 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
      241 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
      301 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
      361 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
      421 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
      481 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
      541 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
      601 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
      661 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
      721 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
      781 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
      841 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
      901 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
      961 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     1021 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     1081 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     1141 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     1201 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
     1261 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
     1321 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
     1381 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
     1441 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
     1501 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
     1561 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
     1621 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
     1681 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
     1741 cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta
     1801 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcatgaat
     1861 cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa acacactgga
     1921 ggatttttca tg
//
LOCUS       DACDKJ010000161         1894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDKJ010000161 DACDKJ010000000
VERSION     DACDKJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.168"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..529
                     /locus_tag="IV328_002996"
                     /pseudo
     CDS             1..529
                     /locus_tag="IV328_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            749..1045
                     /locus_tag="IV328_002997"
                     /pseudo
     CDS             749..1045
                     /locus_tag="IV328_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            1208..1654
                     /locus_tag="IV328_002998"
     CDS             1208..1654
                     /locus_tag="IV328_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939049.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1656..1793
                     /locus_tag="IV328_002999"
     CDS             1656..1793
                     /locus_tag="IV328_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8939050.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
BASE COUNT          678 a          339 c          400 g          477 t
ORIGIN      
        1 cgaagaaggc tcaacagcca caccgtacca gcctaatcta cttgatgcac cgtattattt
       61 gagtaaggtg gctttgggtg agaatctaat taaaccagaa tcacaacaac cagttactaa
      121 tagtaactat cttattaaca cctataacat taaaccaatg gtaaaaggta agaagtatac
      181 catcacactt gaaggaacta agccagcaac acaggttttt agaccatttt tcacacgagc
      241 aacaggagat gcatgggagg ttggtgactt acaaccagta gaagggttaa ctgatgtttg
      301 gtctggaaca tttacagcag cagctgaccc acaccctacc agcccacaag ttcagatttg
      361 tcaggtacca agcacaagtt taggaaactg tacaattaaa tggttaaaac tagaggaagg
      421 cgacacacga accccgaata ttgagcaata taaataccga ggaatcggca tgcgagactc
      481 aaacaatcca aaagattacg tctgggatct agcaccagaa tatgtcgaag acaatctggc
      541 cacagatatt aaaatttctg aaattactgg taaagcaaac aattataccg atgggaaagt
      601 atcggagatt aattcgcagt tgactgcttc aattaatgaa gtagacacca cagctaagga
      661 tgctcaaaca aaagcgaatg ctaatgcgac tgctatagat gaattagaca ataagatcga
      721 tgaacgcatt aatgatacag ctactaccac attaacagtt acaaacggga ataccggatc
      781 agcaaagctt tatcgtgaag gaaaaacagt ttctatatat tttgtggctt taaacggaag
      841 aagcagtggt ggaaatgatt caacgatact aacaattcca gaaggctatc ggccaccaat
      901 tagttttgag caactggttg gctcgataga ccgttctact ttgaacagtg ctcagttatc
      961 tattggtgca gatggagcca ttaaatggcg aagaaactca agttatggat cggattatac
     1021 ctttgcaatt acttacacga tttagaaaag cgtgaatcga tatgaaggca gcatatagac
     1081 caattgaacc ttacggattc gagcaaatca ttgtgaatga tgaagaacat ttaccggaag
     1141 aatgcacaga agtcgaacca ccgattccaa attggaaacc gaaattcaat tactgggagg
     1201 gaaataaatg aaaaacattt ggaaatatgg acgtactggc ggagagtacg caggaaaagt
     1261 attggacgac atgcttgtat ccgttcctta cacagatcag ccaccgcttg aaggaattcg
     1321 tgctgatggc gaaccgttaa caatcgctga tcaaatgttt gatcctaaat tgaatcaatg
     1381 gattatttta gcgaacgcac tagatcacaa cgatttaaac aatctcaaag caatgtatga
     1441 gtcgttagaa aatgagaacg gcgatttaaa acagatcaat gccaaactca tgctaagcga
     1501 tgtagcaatt aaacaggaaa atactgcatt gaaagaaaaa gcggatagtt tagcacaaat
     1561 caattcaaaa atgatgcttg cttcgttaca aaatagcaaa gacatttcag aaattaaaga
     1621 gcaactaaat ccagcttcaa agggaggtga gtagtatgtt tagttttagc gatgtgaaaa
     1681 tgatgtatga ttggggctgt ttcactaacg aacaagtaat ggttttcgtt ccgttgtgca
     1741 ttactgaaga aaaagcagat aaaatcatta gcaaagaaga gagcgcatct taattgatgt
     1801 gctttttatt ttgattcaag gagttgtcac atgattaatt taggggaatg gggagcgata
     1861 gcaggatcaa taaccgctat cgtttctttg attt
//
LOCUS       DACDKJ010000162         1890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDKJ010000162 DACDKJ010000000
VERSION     DACDKJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.169"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IV328_003000"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IV328_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IV328_003001"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IV328_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8939051.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKIFRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          568 a          355 c          367 g          600 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatatttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta
//
LOCUS       DACDKJ010000163         1855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDKJ010000163 DACDKJ010000000
VERSION     DACDKJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.170"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(165..302)
                     /locus_tag="IV328_003002"
     CDS             complement(165..302)
                     /locus_tag="IV328_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8939052.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(304..750)
                     /locus_tag="IV328_003003"
     CDS             complement(304..750)
                     /locus_tag="IV328_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939053.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(910..>1855)
                     /locus_tag="IV328_003004"
     CDS             complement(910..>1855)
                     /locus_tag="IV328_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939054.1"
                     /translation="EEGSTATPYQPNLLDDPYWVGKTSLGENIANKGTTFPIKSSAYE
                     IYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFT
                     PTKLEPGLPKDLRIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNP
                     NDYSWDITPEYTEKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTL
                     TVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSI
                     DRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          479 a          390 c          333 g          653 t
ORIGIN      
        1 cttacttttg aaagagtctc agtaattttc gagaaagatg cagcaatcgg tctaattact
       61 aataaaatca aagaaacgat agcggttatt gatcctgcta tcattcccca ttcccctaaa
      121 ttaatcatgt gacaactcct ttaccttaaa taaaaacgca tcaattaaga tgcgctctta
      181 tctttattaa tgattttatc tgcttcttcg tctgtaatgc atagtggaac gaatagtcga
      241 acttgatcgt cagtaaaaca gccccaatca tacatcattt tcacatcgct aaaactaaac
      301 atactactca cctccttttg aagctggatt tagttgctct ttaatttctg aaatgtcttt
      361 gctattttgt aacgaagcaa gcatcatttt tgaattgatt tgtgctaaac tgtcagcttt
      421 ttctttcaat gcagtatttt cctgtttaat cgctacatcg cttagcatga gtttggcatt
      481 tagctgtttt aggttgccgt tttcatgttc aagagcctcg tacatcgctt tgagattgtt
      541 taaatcgttg tgatctaacg cgttcgctaa aataatccat tggttcaatt taggatcaaa
      601 catctgatca gcaatcgtta gcggttcgcc atctgaacga accccttcga gcggtggctg
      661 atctgtgtaa ggaacggata caagcatgtc gtccaatact tttcctgcat actctccgcc
      721 agtacggcca tatttccaaa tgtttttcat ttatttccct cccagtaatt gaatttcggt
      781 ttccaatttg gaatcggtgg ttcgacttct gtgcattctt ccggtaaatg ttcttcgtca
      841 ttcacaatga tttgctcgaa tccgtaaggc ttgattgatt tgtaaactac tttcattaga
      901 tttcctccct taaattgaat aagtgataac aaatgaataa gctgatccat aacttgagtt
      961 tcttcgccat ttaatggctc catctgcacc aatagataac tgagcactgt tcaaagtaga
     1021 acggtctatc gagccaacca gttgctcaaa actaattggt ggccgatagc cttctggaat
     1081 tgttagtatc gttgaatcat ttccaccact gcttttccca ttcaacgcaa aaaaataaat
     1141 agtgacggtt tttccttcac gataaagctt tgctgatccg gtattcccgt ttgtaactgt
     1201 taaggttacg acttcatatt tgttgtcgtc tgatgtcaat acagattttc cttttgattg
     1261 aataccatct tcgaaatttt tcaatccttc gatggattgc ggttcagtca aactaaccgt
     1321 atcattcaag cctttttcag tatattcagg cgtgatgtcc cagctgtagt cgttcggatt
     1381 gttgctatct ttcaatcctt caccaaagta tttaaactga ctaatattcg gggttcgggt
     1441 gtcgcctttt tcaattttga gccagtcaat ttggcatgcg ccggccgttt ctttaggcaa
     1501 ctgaaaaatg cgaaggtctt taggcaaacc cggttcaagt ttcgttggtg tgaatgttag
     1561 agaccatacg tctgtcaatc cctcaactgg ttttagttct ccaaaattaa cgttccaata
     1621 attatatgct acaaaggttt gacttgcggg ttttgttcct ttaagcgtga tagtatacgt
     1681 ttgacctatt ataagctctt cttccgtgtt acctttatat atttcgtacg cgctagattt
     1741 aattgggaac gtcgtacctt tatttgcaat attctcaccc aacgatgttt tacctaccca
     1801 gtaagggtca tctagtaagt taggctggta cggtgtggct gttgagcctt cttcg
//
LOCUS       DACDKJ010000164         1804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDKJ010000164 DACDKJ010000000
VERSION     DACDKJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.171"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..354
                     /locus_tag="IV328_003005"
     CDS             <1..354
                     /locus_tag="IV328_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8939055.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..903
                     /locus_tag="IV328_003006"
     CDS             355..903
                     /locus_tag="IV328_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939056.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1163..1533
                     /locus_tag="IV328_003007"
                     /pseudo
     CDS             1163..1533
                     /locus_tag="IV328_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1533..>1804
                     /locus_tag="IV328_003008"
     CDS             1533..>1804
                     /locus_tag="IV328_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HAP8939057.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYR"
BASE COUNT          697 a          231 c          380 g          496 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacagagtag aggaagaaca
      541 aaaaaagttg gcgcaccaat cagaaagttt gataaggttt attccaccaa gcatccttta
      601 aaactgtcga ctttgttaaa tgcgacttcc agagttttag tagaaaagta tgagactaat
      661 atgacaagag atgaattgct tagaaaagct ttagatgagt atattaaaca gaatcttacg
      721 caagaagata agcaagattt gtttaatgac gtggtgaaag aactagactt gttcagagaa
      781 aaatatccaa ttgttccaga attagatgat cgtggagaaa taataagaac taccagtcag
      841 ataaaacaag ataccgaaga ctcactgcgc aaagggtggg gaatgaataa taaagttaga
      901 taataatcaa agaagaacta agtaactacc tttaaacggt agttacttag ttcttctttg
      961 atttgcgtga aatagagggg atttattttt ctggttacgt gattaaaaaa tccaaaaaat
     1021 gagcatagga acggttatga ttttagggtg tgttttaatg agatattgtg atttcattaa
     1081 caagtgtatg ataatctgtt gttgagttag taaataagta atagagctaa ctctaaaata
     1141 aaactattag gaggagatgt aggtgaaaaa gtttaaaaag aaaatatatt taggattggt
     1201 tgttggggtg agtatgtttg ggttcatggc tcatactaaa caagtaactg cagatactaa
     1261 ccaaaaagaa aggattatga gccaattaga tgactctcaa ataagtatgt acccaatgag
     1321 ctaattgtca gtttaaaggc tacacaaaga atttcaacag agaaaaataa accgcaaaat
     1381 ttattgggtg taaaagttat ggtggaaaaa gatttgactc ttaggacaaa tgaaagcgat
     1441 tgtaataagc gagatcaagg gttctgttgc aaagttttcc aaaaaatcta ttttagtgta
     1501 aaattgagaa aaaagacaga gaggacagag taatgaatca ttttaaaggc aaacaattca
     1561 aaaaagacgt cattattgtc gctgttggtt actacctgcg ttacaatcta agctatcgtg
     1621 aagttcagga attgttatat gatcgtggaa taaatgtttg tcatactacg atttatcgtt
     1681 gggtacaaga gtacagcaaa gtcctctatt atctttggaa gaagaaaaat agacaatcct
     1741 tctattcatg gaaaatggac gaaacctata tcaaaattaa gggacgttgg cattatcttt
     1801 atcg
//
LOCUS       DACDKJ010000165         1799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDKJ010000165 DACDKJ010000000
VERSION     DACDKJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.172"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IV328_003009"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IV328_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8939058.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DACDKJ010000166         1724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDKJ010000166 DACDKJ010000000
VERSION     DACDKJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.174"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..85
                     /locus_tag="IV328_003010"
     CDS             <1..85
                     /locus_tag="IV328_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8939059.1"
                     /translation="AFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            82..1101
                     /locus_tag="IV328_003011"
     CDS             82..1101
                     /locus_tag="IV328_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8939060.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            1290..1363
                     /locus_tag="IV328_003012"
     tRNA            1290..1363
                     /locus_tag="IV328_003012"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1324..1326,aa:Ile,seq:cat)
BASE COUNT          574 a          303 c          344 g          503 t
ORIGIN      
        1 cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc tacaaaatgt tctcggctga
       61 aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta ttactgcgat gagcctgtta
      121 acggctcttt ttttattgcc aattaacgga tttgcctata ctatcaacaa tgaatttaat
      181 ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc agtatatttt actgcatgaa
      241 acggctaatg aaacagcaac aggacgcaat gaagcgcagt atatgcaacg ttcatggact
      301 agcgcttata ctgcttatat tgtgggagac ggcggaattg tttaccaagt cggacaacct
      361 ggttatgtac agtacggtgc tggttcatat gctaatgcca atagtcctgt gcagattgag
      421 ttacaacaca cacatgataa agcaactttt gaaaaaaact ataaagcata cgttgaattg
      481 gctagagatt cagcaatgaa atatggtatt ccattaacat tagacacgcc ttataaccaa
      541 ccaggaatca aatcacattt atgggtaact caaaacattt ggggcgatca cactgatccg
      601 tatggatact tatcagaaat gggtgtaagt aaggaaaaac tggcttacga tttagctcat
      661 ggatttacag acgaaaaccc aacgacttct gagaacaagc ctgtcattga tccaacccga
      721 gcaggtgcag caaatccaac tttatcagat ggaacgaacc attctcacat tgatcagttt
      781 ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc acatcgctaa ctataagtac
      841 gagtatattt tcattatgga ctacaatact gggaaagaat tagctcgagt aagagctgat
      901 ggaatttata gaccggatgt aaatcaagct tataatactt ctggaaatgt tggctatcat
      961 gtatctttta acatgcgtaa cttccctaat aagaaagtct atgtcatgat gcgggcaacg
     1021 aatgatccag agggaaacac taaaggcggt gcgcaagatt tccatgacaa acgttggtat
     1081 ttaaatattc ctaaacgata aaaatagctc ctcgttgagg agcattacat aactatattg
     1141 acaactataa aaatcattcg ataaaatagt gatgttatcg catatcttca ctatcaccca
     1201 taatagtcac actccaagct atgcgataac aggtttgttg ccacacattc tactggttga
     1261 ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc agagcagacg
     1321 gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt agccatttga
     1381 atcgttctgt gttagaattt tttgaagagt attatacaag ctaaagcttt tcttcattgc
     1441 cactcaaatg agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca
     1501 tagttatatt tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg
     1561 ggttatattg tgagataata ataaagaaga gtttaaagcg ttccccaaaa accactgccc
     1621 cataagtgtg ttacgcttta aactctttta tatttgaagc cattaaaaag cataccatat
     1681 ttttgaaaaa aagtgagaaa aaaggcttat aattggagtg gtag
//
LOCUS       DACDKJ010000167         1697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDKJ010000167 DACDKJ010000000
VERSION     DACDKJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.175"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1581..>1697
                     /locus_tag="IV328_003013"
     CDS             1581..>1697
                     /locus_tag="IV328_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8939061.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIY"
BASE COUNT          642 a          206 c          337 g          512 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taacccataa atgacctaca aacatcttac catagacgaa ctgacaatga
     1621 tagaatcata ttatcttcaa cataataagc cggttgaaat cgctaaccga atgggacgtg
     1681 ctatacaaac tatttat
//
LOCUS       DACDKJ010000168         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDKJ010000168 DACDKJ010000000
VERSION     DACDKJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.176"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IV328_003014"
     CDS             131..505
                     /locus_tag="IV328_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8939062.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IV328_003015"
     CDS             complement(619..1248)
                     /locus_tag="IV328_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8939063.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IV328_003016"
     tRNA            complement(1382..1454)
                     /locus_tag="IV328_003016"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACDKJ010000169         1639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDKJ010000169 DACDKJ010000000
VERSION     DACDKJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.177"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IV328_003017"
     CDS             <1..153
                     /locus_tag="IV328_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8939064.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IV328_003018"
     CDS             159..575
                     /locus_tag="IV328_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939065.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IV328_003019"
     CDS             681..944
                     /locus_tag="IV328_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8939066.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="IV328_003020"
     CDS             944..1216
                     /locus_tag="IV328_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8939067.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="IV328_003021"
     CDS             1293..1622
                     /locus_tag="IV328_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8939068.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          625 a          207 c          324 g          483 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaac
//
LOCUS       DACDKJ010000170         1634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDKJ010000170 DACDKJ010000000
VERSION     DACDKJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.178"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            101..868
                     /locus_tag="IV328_003022"
     CDS             101..868
                     /locus_tag="IV328_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8939069.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="IV328_003023"
     CDS             1060..1458
                     /locus_tag="IV328_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8939070.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          592 a          212 c          315 g          515 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     1621 cctttttatt ttgt
//
LOCUS       DACDKJ010000171         1587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDKJ010000171 DACDKJ010000000
VERSION     DACDKJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.179"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1097)
                     /locus_tag="IV328_003024"
     CDS             complement(<1..1097)
                     /locus_tag="IV328_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8939071.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..>1587)
                     /locus_tag="IV328_003025"
     CDS             complement(1227..>1587)
                     /locus_tag="IV328_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8939072.1"
                     /translation="KLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTG
                     DIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIIS
                     SFNILRRNPKELLIDNN"
BASE COUNT          492 a          321 c          265 g          509 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taatttt
//
LOCUS       DACDKJ010000172         1551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDKJ010000172 DACDKJ010000000
VERSION     DACDKJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.180"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            62..676
                     /locus_tag="IV328_003026"
     CDS             62..676
                     /locus_tag="IV328_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8939073.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(841..>1551)
                     /locus_tag="IV328_003027"
     CDS             complement(841..>1551)
                     /locus_tag="IV328_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939074.1"
                     /translation="ISLELKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLK
                     RSKNISNTRDRFISIITACEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYF
                     NIEFPKEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFM
                     YRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQD
                     NKDFVENIDKEPTNTVGD"
BASE COUNT          511 a          263 c          250 g          527 t
ORIGIN      
        1 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
       61 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
      121 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
      181 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
      241 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta
      301 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
      361 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
      421 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
      481 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
      541 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
      601 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
      661 ggtcaatgac aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac
      721 atataattac tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa
      781 caaaccattt tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg
      841 ttaatcccct actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc
      901 ttgtgattta gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat
      961 agctagttct tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt
     1021 atttggttcc gttggagtca attctataga ttcttcataa aaatctctat acataaagct
     1081 atcattaatt ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca
     1141 ttcttctata gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat
     1201 gaaattttta atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa
     1261 catgctccct aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa
     1321 atcaggtttc tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga
     1381 aataaaccta tctctagtat ttgatatatt tttacttctt ttaaggagta taagagaaaa
     1441 ggggatactt tcgtgtccat gcatgtaatt aactaattgt tgagcttcag aagttatatc
     1501 tctaattata tagttaacgg aattaggaag tgtctttaac tctaatgaaa t
//
LOCUS       DACDKJ010000173         1532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDKJ010000173 DACDKJ010000000
VERSION     DACDKJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.181"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            17..1162
                     /locus_tag="IV328_003028"
     CDS             17..1162
                     /locus_tag="IV328_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP8939075.1"
                     /translation="MEILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGF
                     NSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQK
                     GITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIP
                     LKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVV
                     TDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEE
                     ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKY
                     TKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          570 a          208 c          253 g          501 t
ORIGIN      
        1 ttttactaca gaaattatgg aaatactaat caataaaggt gatttggatg atttgtttcg
       61 tcgtcaccta gaattggcta ttaacgcttt attacaagct gaattaacgg tatttcttga
      121 ctatgaaaag tatgatagag ctggatttaa ttcaggtaat tcccgtaatg ggaattattc
      181 gcgctctttt aaaacagagt acggtgaatt gaatttagta atacctagag ataggaatgg
      241 agacttttct caacaaactt tacctgctta taaaagaacc aatgactctt tagaaaccac
      301 tattattcag ctagttcaaa aaggaattac tatgtccgaa atctctgatc taatcgaaaa
      361 aatgtatggt cattattaca aaccacaaac tatttcaaat atgagtaaaa ttgtgtctga
      421 agatgtttcg gcatttaaag aaagaacttt agaagctaaa tactcagtca tttttatgga
      481 tgctactcat attcccttaa aaagacaaac cgtatcaaaa gaagctgtct atatagtaat
      541 tggtatcagg ttggatggaa ctaaagaggt tctaggattt actatagctc caactgaatc
      601 tgcttatgtt tggaaagaga ttcttcaaga tctaaaagac cgtggtttag aagaggtttt
      661 gttagtcgta acagatggtt taagtggtat tgacgatagt attcatagtg tttatccaaa
      721 tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata aggttcgtgt
      781 tagtgatcga caagaaatct gtagcgattt caaattagtt taccaagcaa gttcaaaaga
      841 agaagcaaat aatcaaataa ggtttatgat agataaatgg aaaaagcagt atccacgagt
      901 ggttaaattg ctcatgaatc ctgctatatt aaccttttat aatttccctc cgtcaatcag
      961 aagaactatt tattcaacta atttgattga aggttttaac aaacaattaa agaaatatac
     1021 caaaagaaaa gagcaatttc ctaatgaaga atctctggag agatttctag tatctcaatt
     1081 caacaactat aatcaaaagt ttctatgccg agttcataaa ggttttaaag aaatacatga
     1141 tacattagaa tcaatgtttt aacttgatca acagaatggg cttttccatt tacacataat
     1201 tattgacaca acctattctg gaaacttaca aataaaagct tattcttcag ccaccttcgg
     1261 gtggttattt tttataaaaa aatgtaaaaa aatattgata cataaataaa ttagttgtag
     1321 taaatagttt tagaattgaa tttctctatc ctttttaaat aattttttag acaaaaaaca
     1381 gaaagtgtct aaagaaagct tgacgaagaa aataattttt aataacttat ttaaggaaat
     1441 tttttgaagg agaagataaa agtgagaaaa aagattatta ttaatgctgg agtaattata
     1501 ggattgatgt tagtaatttt ctccggaaag ca
//
LOCUS       DACDKJ010000174         1530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDKJ010000174 DACDKJ010000000
VERSION     DACDKJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.182"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IV328_003029"
     CDS             <1..395
                     /locus_tag="IV328_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939076.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="IV328_003030"
     CDS             398..1147
                     /locus_tag="IV328_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8939077.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="IV328_003031"
     CDS             1159..1428
                     /locus_tag="IV328_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939078.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
BASE COUNT          607 a          253 c          304 g          366 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca
//
LOCUS       DACDKJ010000175         1496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDKJ010000175 DACDKJ010000000
VERSION     DACDKJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.184"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(523..879)
                     /locus_tag="IV328_003032"
     CDS             complement(523..879)
                     /locus_tag="IV328_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8939079.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(893..1303)
                     /locus_tag="IV328_003033"
     CDS             complement(893..1303)
                     /locus_tag="IV328_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8939080.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          468 a          265 c          214 g          549 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcag
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacaac tcagttatct tatcatgtca
      241 tttctaacat gtcaacaact attttatatg ttttttaaaa tactatttta ttgctttgct
      301 acttaaaaca acagaaatga atatatcatg taaatttaca catgtcaata tatttttaac
      361 tctatatccc cacctagatt tgaattttga ccacataaat tgagcatgtc tctatagtag
      421 aaaaataaag tcaaaaaaag aacacataac acgcttgatg ctaagtgttc gactgttaca
      481 actgtatgaa atgctcagtg acttgattaa aacagttaaa gtctatttat ttaagataag
      541 ctgttccatc aactgctcat tgtatccaat aaatagaact tcatcgataa taatcaaagg
      601 acgtctgagg agattggtat gttgtgtgca taaaaaaatc agttgttttt ttcctacaca
      661 ggaaaggtca atcgcaatgt tgtgaaaagc ctcagaccaa gtcgcaaata gctgttctgc
      721 ttgcacttct tcaaaactgc ataattcttc tgcttcttct gtacttagat cgcgttcttc
      781 taagtcaaca aattgataag gcaattgatg gtgttctaac cattgtttcg ccttcagact
      841 tgttacacag tgcgctgatc catagacttt cacttccacg aaggctccct ccctatgaat
      901 ttaagatgtt attgttttgt aaatagatca gatttctggc aacacatcga atttcacgag
      961 ggacaaatga ccggatctca tcttcattaa atccgattag tattcttttt tcatcaatca
     1021 aaataggtcg tctcaataat gaaggatatt ctttgaataa gacgatcatt tgctttaaag
     1081 ttaattcgtt aaaatctatg cccagctttt cgtaaatttt cgaccgagtg gaaataatat
     1141 cttgaactcc tccttcagtc gctgccaaga tctttttgat ctcactttct gttaacggtt
     1201 cggcaaaaat attcttctct tcaaattcaa gttgattcgc ttgcaaccat gctcttgctt
     1261 ttctacagga agtacagcta ggcgaagtat atacttttat cattttctca ctcattttta
     1321 attataatcc aattctacag taatttcttt taaaatacca attattagct aataatatat
     1381 aaaaaaaaat caaaataact gattatttac gtttgaataa tgagaatttt tctatatatt
     1441 ctctattttg ataatagtat tctcattttc aaaataaata agggaggtgt gacaaa
//
LOCUS       DACDKJ010000176         1468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDKJ010000176 DACDKJ010000000
VERSION     DACDKJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.185"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IV328_003034"
     CDS             287..694
                     /locus_tag="IV328_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939081.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IV328_003035"
     CDS             708..1109
                     /locus_tag="IV328_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939082.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..>1468
                     /locus_tag="IV328_003036"
     CDS             1111..>1468
                     /locus_tag="IV328_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939083.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKII"
BASE COUNT          562 a          178 c          258 g          470 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataa
//
LOCUS       DACDKJ010000177         1453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDKJ010000177 DACDKJ010000000
VERSION     DACDKJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.186"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IV328_003037"
     CDS             complement(<1..625)
                     /locus_tag="IV328_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8939084.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IV328_003038"
     CDS             complement(784..1050)
                     /locus_tag="IV328_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8939085.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IV328_003039"
     CDS             complement(1040..1396)
                     /locus_tag="IV328_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8939086.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          587 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aat
//
LOCUS       DACDKJ010000178         1450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDKJ010000178 DACDKJ010000000
VERSION     DACDKJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.187"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IV328_003040"
     CDS             complement(<1..626)
                     /locus_tag="IV328_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8939087.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1450
                     /locus_tag="IV328_003041"
     CDS             914..>1450
                     /locus_tag="IV328_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8939088.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDS"
BASE COUNT          500 a          212 c          277 g          461 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc
//
LOCUS       DACDKJ010000179         1433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDKJ010000179 DACDKJ010000000
VERSION     DACDKJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.188"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IV328_003042"
     CDS             105..1406
                     /locus_tag="IV328_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8939089.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          287 c          264 g          409 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atg
//
LOCUS       DACDKJ010000180         1428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDKJ010000180 DACDKJ010000000
VERSION     DACDKJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.189"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            65..1300
                     /gene="aph(2'')-I"
                     /locus_tag="IV328_003043"
                     /pseudo
     CDS             65..1300
                     /gene="aph(2'')-I"
                     /locus_tag="IV328_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1284..>1428
                     /locus_tag="IV328_003044"
     CDS             1284..>1428
                     /locus_tag="IV328_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939090.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLR"
BASE COUNT          584 a          121 c          240 g          483 t
ORIGIN      
        1 cccagcttaa atcatagata ccgtaaggga ttttattctt tatttaaaac tttgcaacag
       61 aaccgaatca ttaaaaaaac attatacaga gccttgggaa gatgaagttt ttagagtaat
      121 tattgaatat aacaatgttc ctattggata tggacaaata tataaaatgt atgatgagtt
      181 atatactgat tatcattatc caaaaactga tgagatagtc tatggtatgg atcaatttat
      241 aggagagcca aattattgga gtaaaggaat tggtacaaga tatattaaat tgatttttga
      301 atttttgaaa aaagaaagaa atgctaatgc agttatttta gaccctcata aaaataatcc
      361 aagagcaata agggcatacc aaaaatctgg ttttagaatt attgaagatt tgccagaaca
      421 tgaattacac gagggcaaaa aagaagattg ttatttaatg gaatatagat atgatgataa
      481 tgccacaaat gttaaggcaa tgaaatattt aattgagcat tactttgata atttcaaagt
      541 agatagtatt gaaataatcg gtagtggtta tgatagtgtg gcatatttag ttaataatga
      601 atacattttt aaaacaaaat ttagtactaa taagaaaaaa ggttatgcaa aagaaaaagc
      661 aatatataat tttttaaata caaatttaga aactaatgta aaaattccta atattgaata
      721 ttcgtatatt agtgatgaat tatctatact aggttataaa gaaattaaag gaactttttt
      781 aacaccagaa atttattcta ctatgtcaga agaagaacaa aatttgttaa aacgagatat
      841 tgccagtttt ttaagacaaa tgcacggttt agattataca gatattagtg aatgtactat
      901 tgataataaa caaaatgtat tagaagagta tatattgttg cgtgaaacta tttataatga
      961 tttaactgat atagaaaaag attatataga aagttttatg gaaagactaa atgcaacaac
     1021 agtttttgag ggtaaaaagt gtttatgcca taatgatttt agttgtaatc atctattgtt
     1081 agatggcaat aatagattaa ctggaataat tgattttgga gattctggaa ttatagatga
     1141 atattgtgat tttatatact tacttgaaga tagtgaagaa gaaataggaa caaattttgg
     1201 agaagatata ttaagaatgt atggaaatat agatattgag aaagcaaaag aatatcaaga
     1261 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
     1321 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
     1381 aaaataataa aattatagga tttgcatatt gctatacact tttaagac
//
LOCUS       DACDKJ010000181         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDKJ010000181 DACDKJ010000000
VERSION     DACDKJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.190"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1260)
                     /locus_tag="IV328_003045"
     CDS             complement(<1..1260)
                     /locus_tag="IV328_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8939091.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQY"
BASE COUNT          548 a          204 c          228 g          437 t
ORIGIN      
        1 gtactgataa gtaataacga ctttatttat acctggagga atagttaatt cagttgtgcc
       61 aaacttagat aattttgttt gtactagttt gccattaata gaagccactt gccctttata
      121 acgataaata ggtgtcgtca agacagtatt tttattattc gaattatcta attcaattgt
      181 aagtttgtta ctcataaaat gagttgttgg atttatttcc tggttaccta gtaaatattg
      241 atcattatta atcatatcag gatgatagat tgactctttg tttgcataat cagtatgatt
      301 gtaattttca gctatagcaa cagcgtcttc aggtgctata agtgttaatg aatctttgtt
      361 agcatggata gaattgctta ctcctgagag atgcaatatt agtgcaagaa acgtaaagac
      421 cattattttc catgaattat ttttagtaga aaaaatataa tagatcacta tactcatcgt
      481 ataggctata aataaagaag aaaaaccatt taaccgccag acaaattgta gactcttaac
      541 tggtgtatgg ttaaaaagtt gccaaggaaa taaattggtt gaacaaaaaa gaacaaatac
      601 accaagaaag aaaataaata aatcatcagc agttaatttt tttataaaaa taaaagcacc
      661 aatcaatccg agaaataaga ataatccaat agtatagttg ttatagttat tatttaaaat
      721 atgtgtcagc atatcactag gagccatacc acttaattct tttcccagtg ggactttcaa
      781 ttcttgtgct ctagattgtt gaataaaagg aattaagaat cccgcactta aaaaaaaggt
      841 gaggacggta gcttttaaaa gacttaagag tctagcgatt ttttggtccc aaaaataaaa
      901 agaaagaaac aacgttcctc caataaatac agaaaccata gcgacagaaa gtaaatgagt
      961 atacactact agcgtcatgc caatagacaa ccagtaccat ttttggtaat ctctaatata
     1021 aatttcgtaa caccccatta gtatcaaagg caaaaatgtt aaagcgacag cttctcccaa
     1081 tgaagcacga tggaatatgt caatggcacg ataggcggaa aatgtataaa taatcgaaaa
     1141 taacagcgaa atataacgat tgttttttat ttgtttcata gaaaaataag aaacgaccat
     1201 tgttaaaaaa gtgataaaaa aatagtagat attataactt aaaacaagat ttcccatcat
     1261 tttataaaat aaaaaagctg gataaagagt aagccatgga taaaaaatat tcatcattgt
     1321 tccatgatga tcgaagttat tgaagtttac tggagaactc catacattat ctagtcctat
     1381 gattcgagat aaatgaaagt aagtatcctg attgaca
//
LOCUS       DACDKJ010000182         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDKJ010000182 DACDKJ010000000
VERSION     DACDKJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.191"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IV328_003046"
     CDS             complement(150..1337)
                     /locus_tag="IV328_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8939092.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DACDKJ010000183         1408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDKJ010000183 DACDKJ010000000
VERSION     DACDKJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.192"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(253..741)
                     /locus_tag="IV328_003047"
     CDS             complement(253..741)
                     /locus_tag="IV328_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939093.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(763..1365)
                     /locus_tag="IV328_003048"
     CDS             complement(763..1365)
                     /locus_tag="IV328_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8939094.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT          450 a          323 c          201 g          434 t
ORIGIN      
        1 caattttaca gaatatattc ttgtaaaaaa aaatacagaa agaagcataa gcttctttct
       61 gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa agtcttgccc
      121 gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat
      181 caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt tactttaaca
      241 cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt gaaatccttg
      301 accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat aaacctcgat
      361 tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg tttgcgcagc
      421 aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta ctttatttag
      481 caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag tgtaaccgac
      541 agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt ctttttgaat
      601 ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac caatgatcgg
      661 cacaaaaact tccggccata cttcttgtat gactgccatc agctcatcca gttcattttc
      721 tgttactttt attttttcca tacacccacg tcctttttta cattataaat gaccttgcgc
      781 ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccacg gtttcaagaa
      841 aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta ctagacagat
      901 caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt taatatcttt
      961 cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata aaataagctg
     1021 gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc caatcactag
     1081 gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc ccgctactga
     1141 ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca gaacaaatat
     1201 catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc tcgatcgaaa
     1261 aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac caataatggg
     1321 catgatctga agacattttt gtaaaactga tcctttttta gacatctata ttccccacat
     1381 cttttttcat ttattaaaac tgcctcct
//
LOCUS       DACDKJ010000184         1396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDKJ010000184 DACDKJ010000000
VERSION     DACDKJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.193"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..388)
                     /locus_tag="IV328_003049"
     CDS             complement(<1..388)
                     /locus_tag="IV328_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939095.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKL"
     gene            complement(405..>1396)
                     /locus_tag="IV328_003050"
     CDS             complement(405..>1396)
                     /locus_tag="IV328_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8939096.1"
                     /translation="YMFKTRYYLFGPFRANNIDKVFFKLKMNNLNVAMADRERLYNSL
                     QNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNY
                     DPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEK
                     LAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHL
                     SPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKIST
                     AKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          485 a          237 c          154 g          520 t
ORIGIN      
        1 ttaacttttc tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta
       61 actgatccaa tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg
      121 atattttagc atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa
      181 tatgactgat agattctcta gcttcttcac ataaactttt tactttatta attactactt
      241 tgtcatattc atttaacaca ctattattac cattacttag acgctcagat tctacgaaac
      301 aactcaaatc agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct
      361 ccaaactacg taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg
      421 aaattgtcca ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat
      481 actctcgaaa agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta
      541 tggtttgatt tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa
      601 atcgtaatat gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa
      661 taattagcta tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg
      721 acagaagaaa ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa
      781 taaacaatcc cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt
      841 tcttgtatca aagcatctct actttgatac gcttctatta tatccattcc taatgttata
      901 gacgtttgtg ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta
      961 tctccactaa cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt
     1021 cgaatatctc catttttaac atattccagt attttatttt cataaagaaa cagatatatt
     1081 tctggatcgt aattttgtga taacagatta tctattttga tttgctcgat agttttactt
     1141 agattttctg tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa
     1201 aaataatgaa ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt
     1261 tgcaatgaat tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt
     1321 agtttaaaaa aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg
     1381 gttttaaaca tgtata
//
LOCUS       DACDKJ010000185         1375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDKJ010000185 DACDKJ010000000
VERSION     DACDKJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.195"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IV328_003051"
     CDS             191..445
                     /locus_tag="IV328_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8939097.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IV328_003052"
     CDS             446..763
                     /locus_tag="IV328_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8939098.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          420 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atatt
//
LOCUS       DACDKJ010000186         1362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDKJ010000186 DACDKJ010000000
VERSION     DACDKJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.196"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            112..>1362
                     /locus_tag="IV328_003053"
     CDS             112..>1362
                     /locus_tag="IV328_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8939099.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          426 a          268 c          260 g          408 t
ORIGIN      
        1 ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac
       61 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
      121 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      181 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      241 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      301 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      361 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      421 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      481 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      541 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      601 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      661 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      721 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      781 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      841 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      901 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      961 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
     1021 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1081 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1141 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1201 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1261 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1321 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDKJ010000187         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDKJ010000187 DACDKJ010000000
VERSION     DACDKJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.197"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IV328_003054"
     rRNA            <1..>1355
                     /locus_tag="IV328_003054"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDKJ010000188         1315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDKJ010000188 DACDKJ010000000
VERSION     DACDKJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.199"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IV328_003055"
     CDS             <1..848
                     /locus_tag="IV328_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8939100.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          392 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaatt
//
LOCUS       DACDKJ010000189         1288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDKJ010000189 DACDKJ010000000
VERSION     DACDKJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.200"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IV328_003056"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IV328_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IV328_003057"
     CDS             complement(545..739)
                     /locus_tag="IV328_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939101.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          455 a          217 c          175 g          441 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaa
//
LOCUS       DACDKJ010000190         1277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDKJ010000190 DACDKJ010000000
VERSION     DACDKJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.201"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(44..1183)
                     /locus_tag="IV328_003058"
     CDS             complement(44..1183)
                     /locus_tag="IV328_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8939102.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT          361 a          220 c          187 g          509 t
ORIGIN      
        1 ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc aaactagtta tcatttttat
       61 ataaactatc gaacacagta ttgatttttt ctttatcctg atcttcaagc tctcgaacaa
      121 tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc
      181 taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt agtccatgca
      241 aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat ttgctgacgg
      301 cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaaattatga gggtttttaa
      361 cttcaaaatt ctcgaataat tctttttgat tgattttaaa cttttttaag agatcaatcg
      421 tatgtttatc aatggaaatt ttcctatttg aagtttcgtt ttttgttttt ccccattcat
      481 ttaatttata attccatgtt ttattaatag tgatgatttg atcttcgaaa tcgatatctt
      541 cccatgttaa tcctagaagt tcagcaaatc gcattccagt agcaccagca actaacacga
      601 gcataggaga agagtactgc gcagaaaggt tttcttctac gagtttcatt aaagatttaa
      661 attgatcata atctaaatat ttatcttctt ttggcttcaa tgaatctttt cctttgatta
      721 ctgcttttct agttggatca aacggaatta atccttcttc tacggcatct ttcaacgatg
      781 ctctaatatg gttattaaac ttaataacgg tggattttac atggtctttc gcatacttat
      841 ttaaaaagcg ttgatatcca actctatcta aatcagaaat caataccgct ggcatatatt
      901 tctttatgtt cattaacgta tcttcgtatt ttctataagt gataggagat actgttcctt
      961 ctttataaag ttgcatccaa tcctcaaagt aatctgataa taatagattt ttgcgatccc
     1021 ctttaaggcc tttgttcaag ttatattcta attcgttggc ggcatctttt gcttcgcctt
     1081 ttgtcttaaa tcctgatttt cttatcttcg tgtattttcc gtcatctttt ttgtatgaaa
     1141 tttcgtattg ccaactattt ccacgtttga ctaatcttgc cataattgaa tctactctct
     1201 ttctctgata caataggcac tataaagaag cctactgtat aggtttgttt ttcttagaac
     1261 acgcttgctt tggacgg
//
LOCUS       DACDKJ010000191         1253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDKJ010000191 DACDKJ010000000
VERSION     DACDKJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.202"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..1091
                     /locus_tag="IV328_003059"
     CDS             12..1091
                     /locus_tag="IV328_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8939103.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          329 a          341 c          354 g          229 t
ORIGIN      
        1 gatgaattca catgacgcaa aaccatgata acacagtttc acgcaaggga aaacacctaa
       61 cctactcaga acgttgccaa atcgccatcc tgaaaaagga aaactattcc aaccgtcagg
      121 ttgcggatgt cctgggctgc gtgccgcaga ccataaacaa cgaaatcaaa cgcgggaccg
      181 ttactcagct taaccgccag aatcagaacg ggaaggtcta cgactattat catgacgtct
      241 acgacgccga tgccgggcag gcggcctacg agaagaaccg cttgaactgc ggcagacggc
      301 ccttatgggc cgacacggac gccttcattg aatgggcgga cgacatgatg ctgttggaaa
      361 aatggtcccc ggacgtggtg gtgggctttg ccagaaaaca cgaattgttc gacccggcca
      421 ttatccctgg gaccaccacc ctctatcagt ggatcgaccg tggcatcatg aaaacgaaga
      481 atatggacct gctagaaaaa ctgtcccgca aaccgaaatc gtcccgtcat ggcaaccgcc
      541 cgaataaacg ggtgctaggg aaatccattg aagagcgccc ggaggcggtt ggtacccgga
      601 aaaccttcgg ccattgggaa gtcgacacgg tcgtaggaaa caagaccaag tcggatgccg
      661 tgctgctgac gttggtggaa cggcagacac gcttcgaggt cattctgaag ctggccggga
      721 aggatgccga atcggtcaac caagcggtcc gacagctgag agaacgggct ggtgcgtact
      781 tcggccagct gttcaagacg gttacctcgg acaacggctc ggagttcgcg ggactgcacg
      841 atgccttgca ggatgtggca aacatctact tcagccaccc ctatgcttcc tgggagcgcg
      901 ggacgagcga aaaccaacac aagctgattc gccgtttcat ccccaagggc agcccaatcg
      961 gcaacgtgag cgaaaaccaa atcttgcgga ttcagcgttg gatgaacgat tacccgcgaa
     1021 agattttggg ctatgcaacg ccgcatgaca cgttcgtcca ggcatttagg caggaacggt
     1081 tgacggcttg acacgtgggc ccttgacaac gagcctccct cggcatggct tgtgtctgga
     1141 aagctggccg gggccagcca gtcgggagac taccatgcct cagctcattg tcaagggccc
     1201 actcgattag aaaaattaga cggttggtgg taacgagcaa ccgggtcacc atg
//
LOCUS       DACDKJ010000192         1253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDKJ010000192 DACDKJ010000000
VERSION     DACDKJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.203"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..834)
                     /locus_tag="IV328_003060"
     CDS             complement(<1..834)
                     /locus_tag="IV328_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8939104.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DK"
     gene            complement(840..>1253)
                     /locus_tag="IV328_003061"
     CDS             complement(840..>1253)
                     /locus_tag="IV328_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8939105.1"
                     /translation="GDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSL
                     FEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHR
                     DSERSILIKADYNIQTAYKILESKYYELFELIQRG"
BASE COUNT          383 a          236 c          144 g          490 t
ORIGIN      
        1 tttatcaaat gtctgtgcta tttcatgatt tattctctgt gctaaaacaa tcatataatc
       61 tatcatcaaa taatccacca tcatatcatt atttttccaa taatttaaaa aaaagtctct
      121 aattatagca aacatccatc tagactcttg attacatgct aatgaataac ccgcaaaata
      181 tcctgatgct acggatgcgt gagcataatc aggcctccta atagaccaaa tatcgatatc
      241 aaaatactca tctggtattg gttttgtaca ataaaatgtg gcgtctaacc acaaacctcc
      301 atgttttgct agcaaagata atcttaataa atcagaataa tttgttctcg taatgattcc
      361 tttgtttttc ttttcttcca cccattttgg tatatcaaca tattgtctat aattatcttc
      421 tgttaaaatt ataacagtat gtttccctgc atttcgtttt attgatttta cacaagcttt
      481 tacaatttcc ggcgattgat ccaatccctg ccaccaacaa acccaaatgc agttagaata
      541 tggtgatttt gattgattaa ttatttgatt ttgactataa tcatatgttt tcaaaaaatc
      601 tgaaaaagtc ttttcataat acttcagcat tacctcatgt ttttttaata aatgttcctt
      661 ctgtttttta ctttcattat atccattacg attcatgact tgaatatcaa ttttagcttg
      721 taacgtggca aatgcttcct tccaagatgt tatctttgca attcgtcttg ttgcattcaa
      781 ttcttcggat atgcgatgta taattctttt tatacttgaa tgtttctgtg tcatattctt
      841 taacctcttt gtattaattc aaataactca taatattttg attctaagat tttgtatgct
      901 gtttgaatat tataatctgc ctttattaaa atcgaacgtt ctgaatctcg atgagagata
      961 tctacatttg caatctctct cgcccattct tttgccgatt gatttaaact tatgaaattg
     1021 gtcttatcac tgaaagcaac ttctgtagga actttctccg aaaagaaaca cggtaaatct
     1081 gcaaattgag cttcaatacc cactaacgga accccttcaa ataatgatgg caacattaat
     1141 acgtcaaatg cttgataaaa tttttctata tcatttctat ttcctaaaaa tttaatatca
     1201 tcatctatgc cacttttctt tgcataattt tttatttctt cttccatatc acc
//
LOCUS       DACDKJ010000193         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDKJ010000193 DACDKJ010000000
VERSION     DACDKJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.204"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IV328_003062"
     CDS             200..742
                     /locus_tag="IV328_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939106.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IV328_003063"
     CDS             832..>1246
                     /locus_tag="IV328_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8939107.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDKJ010000194         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDKJ010000194 DACDKJ010000000
VERSION     DACDKJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.205"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IV328_003064"
     CDS             <1..1244
                     /locus_tag="IV328_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8939108.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DACDKJ010000195         1239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDKJ010000195 DACDKJ010000000
VERSION     DACDKJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.206"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IV328_003065"
     CDS             <1..390
                     /locus_tag="IV328_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8939109.1"
                     /translation="NLIDYLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGG
                     QQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKE
                     VADSADIILELSGKKLKKVNKMNLEVE"
     gene            390..>1239
                     /locus_tag="IV328_003066"
     CDS             390..>1239
                     /locus_tag="IV328_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939110.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSS"
BASE COUNT          528 a          128 c          226 g          357 t
ORIGIN      
        1 aatttaatag attatttatc accaattgaa aatattagat tagtaaataa atcagcagat
       61 gaaagtatct tgtttgaact aggtttagat aaaaaacaaa taaaaagaaa tgttatgaaa
      121 ttatctggtg gacagcaaca aagagtagct attgctagag cattggtatc agatgctcca
      181 ataatactag ctgatgagcc tactggtaat ctagacagtg ttactgctgg agaaataatt
      241 aatatattaa agacattagc taaagataga aataaatgtg taatagttgt aacacatagt
      301 aaggaagtag cagattctgc ggatatcatt ttagaactaa gtggtaaaaa gttgaaaaaa
      361 gtaaataaaa tgaatttgga ggttgaataa tgataaaaaa tgcatttgct tatgtaacta
      421 gaaaaagttt aaaatcatta attatcatat tagttatttt atctatgtca actttaagtc
      481 tcattagttt atctattaaa gatgctacgg acagagcttc aaaagaaaca tttgctaata
      541 taacaaatag tttttctatg gagataaata gacaagtaaa tccaggaaca cctagaggtg
      601 ggggaaatgt aaaaggtgaa gatattaaaa agatatctca aacagatagt atagactcct
      661 atgtaaaaag aataaacagt gttgcagatt tagttgatta tgatattatt gaaactcaag
      721 agactcttgc gaatcaatca cctgaaagag caaagaattt taaaagaaca gttatgttaa
      781 ctggggttaa cgactcaaca aaagaaacaa aatttgtatc agaagcatat aaattagtag
      841 aaggaaagca tttagaaaat gaagataaaa ataaaatctt aatgcataaa gatttagctg
      901 aaaaaaataa ccttaaagtt ggagataaga taaaaataaa atctaattta tttgatgctg
      961 ataatgagaa aggtgcagat gaaacagtag aagtagaaat taaaggtcta ttcgatgggc
     1021 ataatagcgg tggagtaagt gcagcacaag aactatacga aaatacatta atcactgata
     1081 ttcatacagc tgctaaagtt tatggtaata ccgaagatac agctgtatac caagatgcaa
     1141 cattctttgt aaaaggtgat aagaatcttg atagtgtaat aaaagatctt gggaaattag
     1201 atataaattg gagagaatat aatttgatta aaagttcat
//
LOCUS       DACDKJ010000196         1219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDKJ010000196 DACDKJ010000000
VERSION     DACDKJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.207"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1219
                     /locus_tag="IV328_003067"
     CDS             <1..>1219
                     /locus_tag="IV328_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8939111.1"
                     /translation="PGVELTCGENGTHLLVLFDKSACTNDISDFLTKMGIKREDWASA
                     AACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFESENIYAVQTVQK
                     EFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEKNNMTFLSFSDN
                     PHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDFSNFSINENEVL
                     LEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLGKEAELDQFSEI
                     QSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIYEFTQENGLVEK
                     ETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKNEIETCVNEIRK
                     LNWDIVSVKKEIQD"
BASE COUNT          450 a          163 c          239 g          367 t
ORIGIN      
        1 tcctggagtg gaattaacgt gtggagaaaa tggaactcat ttactagtcc tatttgataa
       61 aagtgcatgt acaaatgata tctctgattt cttaaccaag atgggtataa aaagagagga
      121 ttgggccagc gctgcagctt gctcaatgaa atcagtttta gaagttattg attttgctga
      181 gcacgagggc ttgctcgtta ttcctgcaca tatagatgag tttaatggaa tttcttcagc
      241 aaaatttgga aatctaggaa agatatttga atcagaaaat atatatgcag tacagacggt
      301 tcaaaaagaa ttttttgaaa atcgctcaca aatgattccc tcatctaagc gtgacactat
      361 ctatgatagt gtgaatgcca gatatgatgg tagagttgga aaagatactt tggaatcttg
      421 gtataaatca gttgttgagg cagaaaaaaa taatatgaca tttcttagct tttctgataa
      481 cccacactcg aaaggaaatt caaaacatgg actttgggga ataggcacaa gatattccta
      541 tataaaaatg aaggacgaac ctgacttggg atcacttcgc gatgctttaa tgctaggcga
      601 aactcgagtt cattctgatt tttctaattt ctccattaat gaaaatgaag ttttattgga
      661 aaaattaagc ttttctggga ccactcttag cacaaaagaa gttgtagtcg aattcagcga
      721 taatttaact tcaattattg gaggaagagg aacaggcaaa tcttgtatta caagattctt
      781 ggtctatgtc cttggaaagg aagcagaact tgatcaattt tctgaaatac aaagtgatta
      841 tcagaacttt gcacaaattg aacataatgg ctctggaata tttttgaagg atacaattgt
      901 gaaactgaat attttctata aggggacaaa atatcaaata ataagaacac aggatagaca
      961 ttctatttat gaatttacac aagagaatgg tctggttgaa aaagaaacag aacggttgag
     1021 aatgatttcc gataaggtga gtatatactc tcaaaaacaa atttatgaaa tttcaaaaaa
     1081 tcaaactagt atactagaat tactagatgg atataactct gatttgattt cagagtataa
     1141 aaatgagatt gaaacatgtg taaacgaaat aagaaaatta aattgggata ttgtttctgt
     1201 gaaaaaagaa atacaagat
//
LOCUS       DACDKJ010000197         1218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDKJ010000197 DACDKJ010000000
VERSION     DACDKJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.208"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(614..871)
                     /locus_tag="IV328_003068"
     CDS             complement(614..871)
                     /locus_tag="IV328_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8939112.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(864..1133)
                     /locus_tag="IV328_003069"
     CDS             complement(864..1133)
                     /locus_tag="IV328_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8939113.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          400 a          212 c          124 g          482 t
ORIGIN      
        1 taatttttct ttagaataaa aaaaacattt atatcagaat caataaattc tatttttttt
       61 attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg agtggggagt
      121 acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat atataaaaat
      181 tacttaactg tatccaaatt taatatgtaa ataaatcatt aatttttagc taaattctat
      241 gagaagttta tttttgttgt ttttgctaat taactaaaac tagtacaaaa aatttaaaat
      301 agattattaa acctattgct tattctattc agtcatatat ataagcgtca tcctttagta
      361 actaacatcc cacactacaa tagtattata acatatctat tcaacttcaa attgttgaaa
      421 tttaagccat ttatatttac ttttcctcaa ctttagaaat gattttacta accttatatc
      481 cgtaatacgc gtaacaaaca cattcattaa taacaatcaa cactaagcta tattagcttt
      541 ttaaatgact agggtaaata aaataaggat caaaaacttt taagtttctg accctttcct
      601 tacttccgat tggttaatag tgatcttttg cagaataaat aaatatcgtt tcattttcaa
      661 ctctatatat cagtctatgt tcatctgtaa ttcttctgga ccattttcca gataaatcat
      721 gctttaatgg ctcaggtttt cctaatccag caaaggggga acgatcgata tcttttatta
      781 acttgttaat cttttttata ttgcttttgt ttccttgctc atgccaataa agataatcat
      841 cccaagcatc atcagaccaa gccttaatca tcagattcaa cctcgattaa gtcatgtgtt
      901 ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa ttttttccat gacgtaatta
      961 ttagaaagtg ttctcaacgt ttcttgcata gaatcataat ctctttttga taatacaaca
     1021 actgtatctt ctacatcttt acttgttaca ataagtgttt cagcatcctc attaacttgt
     1081 ttcatataac tacgtaaatt ttggcggaaa tttgaataag ctactgcttc cattcctttc
     1141 accccttatt tatttctata aaacaattgt acattatatt gtacaattaa gcaaatgatt
     1201 aatattctaa cttcttat
//
LOCUS       DACDKJ010000198         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDKJ010000198 DACDKJ010000000
VERSION     DACDKJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.210"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IV328_003070"
     CDS             <1..1076
                     /locus_tag="IV328_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8939114.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDKJ010000199         1144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDKJ010000199 DACDKJ010000000
VERSION     DACDKJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.211"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="IV328_003071"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="IV328_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8939115.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          380 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc ccattttcat
     1141 ttta
//
LOCUS       DACDKJ010000200         1132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDKJ010000200 DACDKJ010000000
VERSION     DACDKJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.212"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            166..>1132
                     /locus_tag="IV328_003072"
     CDS             166..>1132
                     /locus_tag="IV328_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8939116.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLE"
BASE COUNT          461 a          109 c          223 g          339 t
ORIGIN      
        1 aataatatat atgatgaaaa agtatggaac ttattaaaga aagatacttt actgtttaag
       61 ttgacatgga aacaatcttt taaaaagcaa aaagatgata aagttacttt ttatgggatg
      121 ttggtagaaa acaaattatt ataaatatta gaggtataaa aagttatgaa acacaaggta
      181 agtgtgatag taccaattta caatgttgaa aaatatttaa gaaaatgtgt ggatagttta
      241 ttgagtcaga cattaaaaga gatagaaatc gtattagttg atgatggttc gccagatgct
      301 tcgggggaaa tagcagatga gtatcaaaaa aaatattcta atgtaaaaac aatacataga
      361 gaaaatgggg ggttaggacc ggcaagaaat acaggaattg agaatgcgac aggagaatat
      421 gtggcttttc ttgattcaga tgattgggta gaaagtgata tgtatgaaaa attatactta
      481 gttgcgtcca aagaaaatgc tgatatagta gttagtggac attgtgattt tgcgaatgac
      541 aaagctatgg tgaaaaagca acatcctttg agtgggaatg tatatgatac gcctaatagt
      601 attaaaaatg tacgaaaaaa tttatttgga catagtccaa aagatcaaga agtagaggcc
      661 ttcccgatgt cagtatgtat gtctttgtat aataaaaatc tgctagataa atatagacta
      721 agatttgaaa aaattttatc tgaagataca atttttaact tgaatgcata tgatgtcgca
      781 aataaaattg cttttacaga atatacagat tattgttata gaaaagaaga acaagagtct
      841 attacaaaaa gtttatctcc aaataagcag acggaattta aagaattttt aataactcta
      901 aaagctatag catcgaaaga agatttagaa tgtcaaatca gagcaaaacg tatggcaatt
      961 gattattgta ggctttatgt gggcttagtt gatgatgcaa atttgggaat taaagagaaa
     1021 agaaagtata taaaaaaatt tattgaagat gagggattaa ttcaatgttg gaagggatat
     1081 cctacaaata cgctaccatt tcaacaaaaa ttttttcata aaatgcttga at
//
LOCUS       DACDKJ010000201         1115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDKJ010000201 DACDKJ010000000
VERSION     DACDKJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.213"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..897)
                     /gene="vanY"
                     /locus_tag="IV328_003073"
     CDS             complement(<1..897)
                     /gene="vanY"
                     /locus_tag="IV328_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8939117.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          160 g          399 t
ORIGIN      
        1 agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat
       61 ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa
      121 gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc
      181 catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac
      241 atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata
      301 acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg
      361 ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata
      421 acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa
      481 gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag catcattgac
      541 catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc
      601 gtatccattt attaattcgt catgtttaga taaattcacg atatctgact tcacactttc
      661 ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc
      721 tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg
      781 attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta
      841 gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt
      901 cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa
      961 acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc
     1021 gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc
     1081 tgaatttaac gaggctgaac tgcctgttga caatg
//
LOCUS       DACDKJ010000202         1107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDKJ010000202 DACDKJ010000000
VERSION     DACDKJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.214"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..1041)
                     /locus_tag="IV328_003074"
     CDS             complement(85..1041)
                     /locus_tag="IV328_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939118.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          360 a          181 c          167 g          399 t
ORIGIN      
        1 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taatattttt agaaaaagca
       61 gtcggacttt ctaaaagaag ttcactattc gatcctgtcg atgatttctt caggaaaatc
      121 gtctatttgc ttgtcaattt tgctgtcttt gtatttctct tcttggatag tcaacaaaat
      181 ctttgtaaga ttcccccagt ctactgctct tggcggatcg agccaaacca ctacttgaga
      241 tgcactttct tcatatagat tattcctatt cgtgattata acatccggga actcaggctt
      301 tcctgcctct atcacttccg tgttcagatt aatgtacaag cttaaatttt tcttgataaa
      361 ttgattatag gcagcaccat aagaaaaatc gatgatgatc tttactggtt cagaaatcag
      421 ttgaatgggt atgtgattga tcaataataa taaataactg aaaaaaagaa aaaatttatc
      481 ttgaagcaac tctttgcgtt ttgacgttag atcatttata tactctatca aatagcaata
      541 aaatgctgga tagttttctc taaaaaaaga aacatcaagt ggaaattcat cagctttatt
      601 aatagtgtaa tgcaaaagtt taaaatgtat tttggttaac tctcttcgga tcaattggct
      661 cgtctcttct cgcaacagat taaactgatt ttcaaaactt tcaatgaatc gttcattcaa
      721 ccattcgatt gtttcatctt cattttttct caagtatttt tgatccaatt tatcatgaag
      781 ataaagccaa gagagtgtct tattgataaa aataacggat ttttctccta atactccttt
      841 taagtggtaa aatcctatat ccgaaaaaaa tagcgactga ttgctagcaa tatctaaaga
      901 atcagcttgt tcagccaaca ggttgattgc taaaaagtgg aataatttct ttttttcata
      961 agcagacaga ggataatact gttttaaatc ttttaacatt ttttctgttt ttataattat
     1021 aggtagcgta tacaattcca caaaacttat ctctgttgag ttaaaaagat tatataaaaa
     1081 aagccgaatg tttttaggat tccctgt
//
LOCUS       DACDKJ010000203         1100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDKJ010000203 DACDKJ010000000
VERSION     DACDKJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.215"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..649
                     /locus_tag="IV328_003075"
                     /pseudo
     CDS             1..649
                     /locus_tag="IV328_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            707..>1100
                     /locus_tag="IV328_003076"
     CDS             707..>1100
                     /locus_tag="IV328_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939119.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRR"
BASE COUNT          351 a          151 c          148 g          450 t
ORIGIN      
        1 gaatttgaaa aaaatttttt ctgtaaaaat atttatagtt tatgcatttg ttagtttatt
       61 atatttattt ccagcattta aaaacatgga aatatttggc ttttatgata cggcatttca
      121 cattaatcgt gcgttaagtt tagaatctat ttttagctcc cctattaatt ttgaaacgtt
      181 tagatcatat ggcatgcaag taaataattt ttacccatgg ttaactctat atcctttatt
      241 tttattgatc aagtttacta atttagcaat aggctataac ctttttcttt atattgttac
      301 gctcattact ctatttatat gtcattatgt aatgtatgaa attaccaaaa aacatgttac
      361 tagctccttt ttcgctatta tatatacaac atcttctttt aggagtgtag aaatatttct
      421 tcgaggggca atgggggaat tactcgcaat gtcaatactt cctcttattt tactcgggtt
      481 tataaagctt tacgactcaa aaaaagaatc ttgggttatg ttggcgatta gcatgacttt
      541 attaatttac actcatgttc tttctgtagc tatgactatg gtaatgatta taattgcttt
      601 aattattcaa ttttatcgga aaaagaaaat agaaatcttc ttaattatta aattaataaa
      661 agctgctata gttactttac ttctttcact agcgtttata ggtcctatga ttgagcagac
      721 attgtatcaa gatctgaata gaccttatgt ggatatatta caaaaaagag caatttatct
      781 gggtaaagag tttttgattg gaagtataga ttcagaatta ctatcatttg gtattggctt
      841 aactttgctt atttgtttaa ttatactaac atttaacttt aaaaaattag gaatcttatc
      901 taaaatcact tatataatgg gtataatatc tattctactt tgtacaaaaa tatttccatg
      961 gtttgcctta caaaatactc ctttaaatat tattcaagct ccttggcgtt ttatgatatt
     1021 ttcaacattg ttcatatctt tttcagtatc tctatttatt tctccacagt tggaaaaatt
     1081 ttcttataca aggagaagaa
//
LOCUS       DACDKJ010000204         1094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDKJ010000204 DACDKJ010000000
VERSION     DACDKJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.216"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            62..>1094
                     /locus_tag="IV328_003077"
     CDS             62..>1094
                     /locus_tag="IV328_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8939120.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAF"
BASE COUNT          362 a          211 c          214 g          307 t
ORIGIN      
        1 tatcgttctc tagtatgatt aaatcaccac aaaataaaca actgagagga cgatacgcta
       61 tatgaatgat tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga
      121 ggtctcttac gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc
      181 tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat
      241 tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc
      301 tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac
      361 ggctcaaagt tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat
      421 tgcttcttta ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc
      481 tttgaccacc gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa
      541 gaagatcctc cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga
      601 taagatgagc tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac
      661 gcgtaaatta cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga
      721 attcttggtg acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa
      781 tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga
      841 gttgcgtatc cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa
      901 actgaaaaag aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata
      961 caaaacatgg aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat
     1021 tgatcgattg cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt
     1081 agttgaagcc tttc
//
LOCUS       DACDKJ010000205         1072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDKJ010000205 DACDKJ010000000
VERSION     DACDKJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.217"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            439..1072
                     /locus_tag="IV328_003078"
                     /pseudo
     CDS             439..1072
                     /locus_tag="IV328_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          371 a          186 c          189 g          326 t
ORIGIN      
        1 gattactcac tatataaaat gtgattaatt ttattattaa taaaatattt tttatattac
       61 aaaagtagtg tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga
      121 aagaaatgaa caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa
      181 aaacaatctt aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt
      241 agcaagctag tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa
      301 tcattatgga tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat
      361 tcgcaaaata taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc
      421 cattgttatt ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag
      481 ccgtcaaaaa ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca
      541 attgtggata aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt
      601 ttgcctacac gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa
      661 gcggtggaat atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc
      721 ttaccaaatg cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg
      781 ttcaatgatt ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa
      841 gccgagaaag tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac
      901 tatgattaca aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca
      961 atgatgattg atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc
     1021 catgagctga ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DACDKJ010000206         1055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDKJ010000206 DACDKJ010000000
VERSION     DACDKJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.218"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            226..>1055
                     /locus_tag="IV328_003079"
     CDS             226..>1055
                     /locus_tag="IV328_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8939121.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          372 a          150 c          181 g          352 t
ORIGIN      
        1 tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac aatattaatg
       61 aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag acaaaatgat
      121 agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag tccattctgt
      181 aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc caattttact
      241 acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt tcgatgtcat
      301 ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct tgattatgaa
      361 aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta ttcacgttca
      421 ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa tggtaagttt
      481 tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt
      541 cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga aaaaatgtac
      601 gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc tgaagatatt
      661 attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat ggatgctact
      721 cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt aattggcatt
      781 cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga atctgcttat
      841 gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt tttattagtc
      901 gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc aaatgctcaa
      961 tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat
     1021 cgacaagaaa tctgtagcga tttcaaatta gttta
//
LOCUS       DACDKJ010000207         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDKJ010000207 DACDKJ010000000
VERSION     DACDKJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.219"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IV328_003080"
     CDS             6..980
                     /locus_tag="IV328_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8939122.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DACDKJ010000208          997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDKJ010000208 DACDKJ010000000
VERSION     DACDKJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.220"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..913)
                     /locus_tag="IV328_003081"
     CDS             complement(26..913)
                     /locus_tag="IV328_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8939123.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DACDKJ010000209          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDKJ010000209 DACDKJ010000000
VERSION     DACDKJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.221"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IV328_003082"
     rRNA            complement(<1..>994)
                     /locus_tag="IV328_003082"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDKJ010000210          992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDKJ010000210 DACDKJ010000000
VERSION     DACDKJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.222"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IV328_003083"
     CDS             complement(55..789)
                     /locus_tag="IV328_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8939124.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          344 a          206 c          182 g          260 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ct
//
LOCUS       DACDKJ010000211          986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDKJ010000211 DACDKJ010000000
VERSION     DACDKJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.223"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..200)
                     /locus_tag="IV328_003084"
     CDS             complement(<1..200)
                     /locus_tag="IV328_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP8939125.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGL"
     gene            complement(297..797)
                     /locus_tag="IV328_003085"
     CDS             complement(297..797)
                     /locus_tag="IV328_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8939126.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            complement(810..>986)
                     /locus_tag="IV328_003086"
     CDS             complement(810..>986)
                     /locus_tag="IV328_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939127.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
BASE COUNT          323 a          249 c          129 g          285 t
ORIGIN      
        1 tctaagccat tttcgatagc cactttctta tattcatcaa tattttcccc tgtattgtga
       61 tcagttaatg caacacaatc gagtcctttg tttaaacatt cttctaccca ttccttagca
      121 gtaacctttt tatcctgaaa gcactgactc gcagttgaat gaatatgaag atcacatttg
      181 taccacttat tgccaaccat tagtaacacc cctatcaaca aatattttct atttcagtat
      241 atcaaataga aaagtaaaag tacgatagaa atttctggtc aattctaaca aatgatttac
      301 attagttcaa aaacccctcg actcgtaacc aaataattcc cgccaatctc caagtctcga
      361 ccacacgcat caaaatcaat atacgtcttc aagtgttctg gtacttcgcc taaatcacca
      421 gcttctacaa tcaagtaata cgctacatca ctcatgtcat tacaatcggg ataatagatg
      481 atatcatcca catgctctac catttcctcg aaagaattga agaaggcatg ttgaatctct
      541 gaagcgacct caccaattgg cgttccttta agctcttccg ctaaaccaca caaccgatta
      601 atttcctcaa ttggtgtata ctcatcaatc tcaaaaggca gttcataatc atgaattgcg
      661 tactcttcat attcgtcatt taatccaatc cgttctttca cttcatcaaa atcgacgggt
      721 ggggtaaacc atgcccccac caactcacct tcattgtatt ttccaaggtt cgcaatataa
      781 acacgtatct gttccattgt gattccccct caggcaccga caattcggtt aaacaaattg
      841 agtaagacat ccttcacacc agaagcatta aagaccagac ctaccgcaat caaagcaatc
      901 accaagaagc caatcagttt ggaaaactcg cgtttaaatc ctaaaaacaa accaatgacc
      961 acaatcgcta gtaagactaa agattg
//
LOCUS       DACDKJ010000212          984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDKJ010000212 DACDKJ010000000
VERSION     DACDKJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.224"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IV328_003087"
     CDS             <1..704
                     /locus_tag="IV328_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8939128.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          332 a          161 c          163 g          328 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aata
//
LOCUS       DACDKJ010000213          966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDKJ010000213 DACDKJ010000000
VERSION     DACDKJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.225"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..461)
                     /locus_tag="IV328_003088"
     CDS             complement(<1..461)
                     /locus_tag="IV328_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8939129.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(745..951)
                     /locus_tag="IV328_003089"
     CDS             complement(745..951)
                     /locus_tag="IV328_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8939130.1"
                     /translation="MNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRVAYEII
                     ENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          312 a          184 c          128 g          342 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa agaagccgtc
      721 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
      781 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
      841 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
      901 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
      961 tttcaa
//
LOCUS       DACDKJ010000214          907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDKJ010000214 DACDKJ010000000
VERSION     DACDKJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.226"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IV328_003090"
     CDS             <1..888
                     /locus_tag="IV328_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8939131.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          119 c          149 g          250 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcag
//
LOCUS       DACDKJ010000215          855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDKJ010000215 DACDKJ010000000
VERSION     DACDKJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.227"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..182
                     /locus_tag="IV328_003091"
     CDS             <1..182
                     /locus_tag="IV328_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8939132.1"
                     /translation="QDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVSTEIKVLMGITA"
     gene            244..>855
                     /locus_tag="IV328_003092"
     CDS             244..>855
                     /locus_tag="IV328_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939133.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKI"
BASE COUNT          308 a          130 c          167 g          250 t
ORIGIN      
        1 aacaagacca tcgacctatt aaacgacgaa ataaatttta tcaaagtctc cgtacagcct
       61 cttccacgat taagggcatg gagaccattc gaggaatata taaaaagaac cgaagaaatg
      121 gaacgctctt cggcttttcg gtgtctactg aaatcaaggt attaatggga ataacagcct
      181 aagatatttg gagttcagag agggcgcgtt tgattttcaa acttcgcaac agaaccgaga
      241 accttgggtt ttcaagtgct ttttggttca ttaatcttaa ccatagtgtt tttaaagtgg
      301 aaaacaagca acaaaaaaag tatgtggagc cttgtaggaa tagttcttat tatttttact
      361 gtgactgcag caacagaagc aaattattcg cataaaattc agtcgtataa aggtcgttta
      421 gttatgacca aaaaagatgt tacacgttat acatcctcaa gaactggagg cctatatgac
      481 tatgcaccat tagatgcatt gaaatataaa gatcatttaa agaaacatga agtaaaagtt
      541 ggtgatcggt gggagaatgc atctcataaa gcatataatt ccaaaatagt ctatacagta
      601 gaagatgccg atgagcaaaa tatcacatta cctgtattca attattgggg aacagttgtg
      661 aaagtaaatg ggaaaaagag cactatagaa aatgacaatg gtttagtgtc tattaaaggt
      721 acggggaaaa gaactatagt tgaggtttca tctacgtatc caactacgat gattctatct
      781 ttattaatta gttcaataac ttttatattc ttatttacac gttatttaat aagatgctgc
      841 ggtgaaatga aaata
//
LOCUS       DACDKJ010000216          838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDKJ010000216 DACDKJ010000000
VERSION     DACDKJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.228"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..524
                     /locus_tag="IV328_003093"
     CDS             <1..524
                     /locus_tag="IV328_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8939134.1"
                     /translation="KISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEK
                     TNLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQS
                     TINSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTA
                     NKAQQIVNEHTIQ"
BASE COUNT          373 a          103 c          115 g          247 t
ORIGIN      
        1 aaaaaattag tattttaaat tcacaattgt cttcaaacaa ccaaactctt gaacaactaa
       61 gaactaatat tgaagtttcc aaacaaaaag tggcatcttt agaaaacgac aagaatcaat
      121 tagtatcaga gaaaactaat ttagaaaatc aattgtcagc aaaagactct gaattgcaag
      181 gaaaacaaaa tgaaattaat gcgaaaatag acgaaattaa ccaaaaagta gctgaaataa
      241 atcgattaaa tagcgattgg aataatcgtt tacaaaatga aaagaacaaa tttaatgaag
      301 aacaatctac tattaatagt ttaaaccagc aaattaatca aatgttgagt gaaaaaaata
      361 acattcaaaa tcaactgcaa gctactcagc aaaagctaga agaagaaaag tcaaaaaata
      421 gtagtttgaa taattacatt gaaaagcttc aaaaagctaa aaatgatgta gaggataccg
      481 caaataaagc acaacaaatt gtaaatgaac atacaatcca atagatatag ttttaattta
      541 acaaatggta tgtttggaga taatgagata gaatataaat gtcataatat tatacaatct
      601 aaacaccgtt tccttttcta tttttgatga catcagtata tagctactcg aaagagtagt
      661 ctttttatta ttctcgctta ttattgatat gaaaatttcg atttgacaaa aggtaccgaa
      721 taattttgta atatgaatta ttatcaagaa gtaattatga tgataacata tggaaactag
      781 ttagtaataa taacaggtga ttaaactttt gaacgacttg taattctata ttttttct
//
LOCUS       DACDKJ010000217          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDKJ010000217 DACDKJ010000000
VERSION     DACDKJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.229"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IV328_003094"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IV328_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8939135.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDKJ010000218          792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDKJ010000218 DACDKJ010000000
VERSION     DACDKJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.230"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(107..>792)
                     /locus_tag="IV328_003095"
     CDS             complement(107..>792)
                     /locus_tag="IV328_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8939136.1"
                     /translation="FRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVS
                     IDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYG
                     ETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVL
                     NIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSI
                     SYLYLKKEN"
BASE COUNT          297 a          137 c           93 g          265 t
ORIGIN      
        1 agaataaata aataaatctc cttttgattc tcggtaaaaa ttttgataaa catttaatat
       61 attaatcaaa tgtaaaggag tccaagcaat gaaagctgtc ttcatattaa ttctcctttt
      121 ttaaatacaa gtaagaaatg cttcccaaat aataccagaa aacaattgaa atgacatcgc
      181 gcttattgaa aagtacatga gcctccacca tttcatctat aaaaatagat agtataaaaa
      241 ttaaaacaat accaagaaac gcataattca ctttacttct aaaaaatgtt ttgagtaaac
      301 taaagatatt taacacatag aaagcataga tacatataaa agcaattata ccacttgata
      361 caagtacttg aagaaatcca ttatgcatgt ttccatgtgc tctttttagt tcattcacat
      421 ctgtagtatc aagatctact tttttcaaaa attttgcatc gctattccca tagaaatcgg
      481 tctctccata accaaataac aaagttggtc ttataacttt aagcgcagct ttccaaatcg
      541 taaaacgacc agctgaaatt tcggagttat cactagaatg ctgtaatatt acttcttttt
      601 ctggaagtgg agttttatct gattcatttg actctataac ttctactttt ctatcaatcg
      661 atacaggaat tgttgctaaa aaatccttag cataattagt accaaaagaa aaatatacca
      721 atagaactaa taatatgctt accatccatt tgatttttac aggtagtact tctctaatgc
      781 tctttctaaa tt
//
LOCUS       DACDKJ010000219          766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDKJ010000219 DACDKJ010000000
VERSION     DACDKJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.231"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(109..>766)
                     /locus_tag="IV328_003096"
     CDS             complement(109..>766)
                     /locus_tag="IV328_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP8939137.1"
                     /translation="IGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQP
                     LEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTD
                     VKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQ
                     FETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA
                     "
BASE COUNT          213 a          175 c          146 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgatt
//
LOCUS       DACDKJ010000220          744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDKJ010000220 DACDKJ010000000
VERSION     DACDKJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.232"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            320..718
                     /gene="spxA"
                     /locus_tag="IV328_003097"
     CDS             320..718
                     /gene="spxA"
                     /locus_tag="IV328_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8939138.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          273 a          104 c          135 g          232 t
ORIGIN      
        1 gaaataaaaa acataaaaaa tcttaagaaa tcggtttcat ttagcttttg agataaaata
       61 atagtacagt tttatacgct gtttcatatg tttagaaaat ttgaattaga tttgaaaact
      121 ctaattttta aatgataaaa aaagcgagaa tgctggtttt actgtatatt aaccttagat
      181 aactttcatt tttttaaaaa aattatgaaa aaaaggtagc tatttaaaag ttgattatgt
      241 ataatagaaa cagatgggag attattatga tcttctgtta caaaaaagtg attcttatca
      301 cgaaaaggag tggtgcacgt tgttaacatt atatacttcc ccaagttgta cttcctgcag
      361 aaaggctcgt gcgtggttgc aagaacatca gattccattt gttgaacgta atattttttc
      421 agaaccattg aacagttctg aattaaaagc gattttacaa atgacagaag acggaacaga
      481 agaaatcatc tcgacacgtt ccaaagtatt ccaaaaactg aacatggatt tagatgatct
      541 gccgttacaa gaattattgg aacttgtgca aaacaatcct ggattgcttc gccgtccaat
      601 catgattgat gataaacgtc tgcaagttgg ttttaatgaa gatgaaattc gtcggttcct
      661 accgcgcgat gtacgacaat tagaattacg tcaagcacaa ttgatggctg gattatagaa
      721 ggataatacg aaacttggaa aaga
//
LOCUS       DACDKJ010000221          733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDKJ010000221 DACDKJ010000000
VERSION     DACDKJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.233"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>733
                     /locus_tag="IV328_003098"
     rRNA            2..>733
                     /locus_tag="IV328_003098"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DACDKJ010000222          710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDKJ010000222 DACDKJ010000000
VERSION     DACDKJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.234"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>710
                     /locus_tag="IV328_003099"
     CDS             <1..>710
                     /locus_tag="IV328_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8939139.1"
                     /translation="HWTAQSYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSL
                     TTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLP
                     TRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQA
                     FNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLT
                     EAMMIDRLLGFSTSLKEA"
BASE COUNT          231 a          137 c          136 g          206 t
ORIGIN      
        1 ccattggacg gctcaaagtt attttgttca acccagacat tcaatcgcga atcatgtcag
       61 atataaaatt gcttctttac tgactgaaaa agtatcttta tcttttattg cgaaaaattg
      121 tcaggtatct ttgaccaccg ttattcgtac gttgaaagag tttaaaagct acttaccaaa
      181 gcaatctaag aggattctcc ccagagtatt gatggttgat gaatttcgtt cgcacgcttc
      241 catagaagat aaaatgagct ttatttgcgc agatggcgaa acaggacaat taattgatgt
      301 cttgcctacg cgtaaattgc ctcgattaac aagctatttc ttaggctgcg ctaatcctga
      361 agaagtaaaa tttttagtca cagacatgaa cgccgcctac ttccagctta ccaaacgtat
      421 tctgccaaat gcgaaagtag tgattgatcg gtttcatatt atcaaacaca tgaatcaagc
      481 ctttaatgag ttgcgtatcc gtgaaatgaa tgaacttcgt aaagtaggac agaaaagtca
      541 ggcagaaaaa ctgaaaaaga actggcgctt tttgctaaaa aatcgtgcaa acatcaacca
      601 ttatgaatac aaaacatgga aaagttttcg agcaccaaaa tacccatttc ttacggaagc
      661 gatgatgatt gatcgattgc ttggcttttc tacgtccctg aaggaggcgt
//
LOCUS       DACDKJ010000223          709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDKJ010000223 DACDKJ010000000
VERSION     DACDKJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.235"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..443)
                     /locus_tag="IV328_003100"
     CDS             complement(<1..443)
                     /locus_tag="IV328_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8939140.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKR"
BASE COUNT          214 a          122 c           95 g          278 t
ORIGIN      
        1 tttctctttt caaaaaaaat ttgatatttt tttagatttc tttttagtct ttgtaatact
       61 ttataagcca cagaataact cacgtgatgc tgtttagcaa aattttctac tgagatatat
      121 ttttcctgaa agaaagccaa taccattttc atagataaag aatccgttat ataacgagat
      181 aacagagctt tgctagaaca ttttccatta atttccagac atatatccaa ttcactgacg
      241 gttactcgca aatttttctc tagatcgaac ttttgtaaat catgtttcag cttatcgatc
      301 gtttttttca agagaaatgt cgatagcgac aactcttctt gaacattcga tattgactct
      361 gataaatttg agcttgcttc taaatattta agtaatgaat aacttctgtg atcttctttg
      421 cttaaaaaaa actcataatt cattagtcat cagctctttc tacgtatatt actttccatg
      481 cattcaccaa ctagctgggg ataaaactga tttcctggat ccgagtcact gttgtcagcg
      541 attcttgttt gtataatata catcgtcctg tctttttttt aacattttgg agagcgtttt
      601 atttggcctt tctcgcgatc aaacctgtta ttagaattat atcatttact agtatgcatt
      661 tttattaata aaatggtata ttttatattg ttataaaaag agcccaacc
//
LOCUS       DACDKJ010000224          696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDKJ010000224 DACDKJ010000000
VERSION     DACDKJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.236"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(54..629)
                     /locus_tag="IV328_003101"
     CDS             complement(54..629)
                     /locus_tag="IV328_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8939141.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DACDKJ010000225          687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDKJ010000225 DACDKJ010000000
VERSION     DACDKJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.237"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          238 a          128 c           91 g          230 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactacaa taatattttt
      601 ctaacttatc gccttcaaag aaatagtttc ttatttttca ctaaaataaa aaatttattg
      661 caaaaaaaat agaagtgaca aaattat
//
LOCUS       DACDKJ010000226          679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDKJ010000226 DACDKJ010000000
VERSION     DACDKJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.238"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(107..511)
                     /locus_tag="IV328_003102"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IV328_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          216 a          125 c          107 g          231 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgtt
//
LOCUS       DACDKJ010000227          654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDKJ010000227 DACDKJ010000000
VERSION     DACDKJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.239"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>654
                     /locus_tag="IV328_003103"
     CDS             <1..>654
                     /locus_tag="IV328_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8939142.1"
                     /translation="QQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIAN
                     QNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCK
                     FECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNS
                     CREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVIL"
BASE COUNT          270 a           80 c          105 g          199 t
ORIGIN      
        1 acagcaattg aaaagaaaag aattggttta tctaaaaaaa tatactgatt cactggaaaa
       61 agatcaagaa aaatttgcca gatttagaca tgactataaa aatttattgc tgagtttaaa
      121 agaaatcgct aatcaaaacc atgattcgat attggaaaaa gaaataaaag aattagagat
      181 ctattctaat tcttatctaa ccaataagtt tgaatatcgt tatttaaaaa atataaaaaa
      241 tgaatatttg aaaagtttgt tgatagcgaa gctataccaa gcaaataaca ataagatttg
      301 ttgtaaattc gaatgtccaa gtactattat tgaagttcct gttccaatat ttgactgtat
      361 tagggtgctt ggaattattc tcgataatgc aattgaagca tctatagaga gtcaagatag
      421 agaactgtct ctagctattt accgagatga taaacagata gaaatttcga tatcgaatag
      481 ttgtagagag atactactct ctatagatac tctaatgaaa aaaggaataa caacaaaaaa
      541 gggacatcaa gggttaggtt tatctagtat agaggaaatc aataaaaaga atcccaatat
      601 gtttgtgaac tatcaaaacg atccgtatca atttactact caagttattt tgtt
//
LOCUS       DACDKJ010000228          612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDKJ010000228 DACDKJ010000000
VERSION     DACDKJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.240"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>612
                     /locus_tag="IV328_003104"
     CDS             <1..>612
                     /locus_tag="IV328_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8939143.1"
                     /translation="FLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFV
                     SNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIV
                     GTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNN
                     YSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLIND"
BASE COUNT          228 a           73 c          106 g          205 t
ORIGIN      
        1 catttttgaa gagaaaagaa attcaatata ttaagtctaa agtgacgcat aataaaaaaa
       61 tttcttatca ataccatgta ggacagtatt tacccgactg gcatccttgg gaagattatc
      121 gcaatttttt tgtttcaaac aaaaaaacta atgggtgtag agaaatattc gctattgaat
      181 taccttggat tatagataca tttgggaaag ttaaatcgtt tactgtaatg acccagaaat
      241 tatccaatct tgatattgat tattcggaca gtttcactct tattattgag catgaaactg
      301 gtatagttgg aactttaagt gtagatgttg tttcaagagt agctaaaaga aatttaacta
      361 taattggtga aaagatttta atagaatggg aaggtaaccc ggattctcta ttttcatgga
      421 atgaagaaac ttctagtatg acagatattc aattgtacga tgaggttatg catgataata
      481 attattcaca tactattatt gaagatgcat atttagagga gattaaagaa tttttcacgc
      541 ttttggaaaa gaaaatggaa ccccaatata cttttgaaaa agataagtat attattaact
      601 taattaacga ca
//
LOCUS       DACDKJ010000229          612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDKJ010000229 DACDKJ010000000
VERSION     DACDKJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.241"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            116..>612
                     /locus_tag="IV328_003105"
     CDS             116..>612
                     /locus_tag="IV328_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8939144.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          230 a          106 c          101 g          175 t
ORIGIN      
        1 tttttgagga gagtataaaa tattttgtgt aaatgaaaaa atccatacaa aaaaggaagt
       61 cccttctgta gaataaagtt aacgacaacc aattcacaga aaagaggact tccctatgaa
      121 tgattttact acagaaattg tgcaaactct agtcactaaa ggcgatttaa atgaattatt
      181 ccgttcgcac ttagaaaaag cgataaacac actcctacgg actgaattaa cggctttttt
      241 agattacgaa aaatatgatc gcactggttt taattcaggt aattcgagaa acggttctta
      301 ctttcgatca atcaaaaccg aatatggtga attaacattg gaaataccta gagatcgtaa
      361 tggtgagttt aaacaacaaa ctttaccagc ctacaaaaga acaaacgata cattggaaac
      421 cactattatc catttattcg aaaaaggtgt tacgatgtct gaaattgctg atttgatcga
      481 aaaaatgtac ggtcatcact atactccaca aaccatgtcc aacatgacta aagttctgac
      541 tgaagaagta aatgccttta aatccagagc cttaaatgat aagtatgtcg ctatttttat
      601 ggacgctact ta
//
LOCUS       DACDKJ010000230          538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDKJ010000230 DACDKJ010000000
VERSION     DACDKJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.243"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..278)
                     /locus_tag="IV328_003106"
     CDS             complement(<1..278)
                     /locus_tag="IV328_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939145.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYT"
BASE COUNT          187 a           90 c           66 g          195 t
ORIGIN      
        1 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
       61 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      121 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      181 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      241 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      301 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      361 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      421 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      481 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaactttt
//
LOCUS       DACDKJ010000231          530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDKJ010000231 DACDKJ010000000
VERSION     DACDKJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.244"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            201..503
                     /locus_tag="IV328_003107"
     CDS             201..503
                     /locus_tag="IV328_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939146.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          173 a           78 c           86 g          193 t
ORIGIN      
        1 actattacac gtcttctgtt gtgtttgtta ttgcaggaaa tcttaatata gaaggttttc
       61 tatttgttta cattttgttg aattcgaatt tttttggcac ataatttggc acaagtactt
      121 gttttctatt attcagtata ttaaattccg ccttcacaaa aagaacattc gttcgtatac
      181 tctttttgag gtgaactttt atgctgatgg aaggaaaaac acaattatgg tttaaatttg
      241 acccttcgaa tagatttatc aaagattttt ataaagtatg gaattcagaa gttttctttt
      301 tagcaatcga agatagctta ttaatcaatc tctactattc taataagaac tatttcaaaa
      361 ttcctgctgc gaaaactaga atgaagaagg gtgtatactt tttgtttgat atcgtgactg
      421 acgtgccgga cgcacgtagc gatcatcggc gttatgacta tataaagtat actttcgttg
      481 atccagaaag atacaaagat taaataaaaa aataacaaac aaactcgctt
//
LOCUS       DACDKJ010000232          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDKJ010000232 DACDKJ010000000
VERSION     DACDKJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.245"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IV328_003108"
     tRNA            complement(5..90)
                     /locus_tag="IV328_003108"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IV328_003109"
     tRNA            complement(107..178)
                     /locus_tag="IV328_003109"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IV328_003110"
     tRNA            complement(190..262)
                     /locus_tag="IV328_003110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IV328_003111"
     tRNA            complement(280..361)
                     /locus_tag="IV328_003111"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IV328_003112"
     tRNA            complement(369..441)
                     /locus_tag="IV328_003112"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IV328_003113"
     tRNA            complement(445..517)
                     /locus_tag="IV328_003113"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDKJ010000233          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDKJ010000233 DACDKJ010000000
VERSION     DACDKJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.246"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IV328_003114"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IV328_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8939147.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDKJ010000234          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDKJ010000234 DACDKJ010000000
VERSION     DACDKJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.247"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..510
                     /locus_tag="IV328_003115"
                     /pseudo
     CDS             1..510
                     /locus_tag="IV328_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DACDKJ010000235          498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDKJ010000235 DACDKJ010000000
VERSION     DACDKJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.248"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..132
                     /locus_tag="IV328_003116"
     CDS             <1..132
                     /locus_tag="IV328_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8939148.1"
                     /translation="IRSIQREIDDLERTKAVMVNETARKAIDLHIENLRREIRRLEG"
     gene            135..434
                     /locus_tag="IV328_003117"
     CDS             135..434
                     /locus_tag="IV328_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939149.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT          189 a           74 c          129 g          106 t
ORIGIN      
        1 attcgaagca ttcaacgtga aattgacgat ctggaacgga caaaggcagt gatggtcaat
       61 gaaacggcga ggaaggcaat cgatttgcac atagagaatt taagaaggga aattcgtaga
      121 ttggaggggt aaggatggat aagaaagcag caatgaaacg aatcatcgaa ctgacacatt
      181 ctgagaattg gcaagaagac aaagaaatag ttgcagaagt ccaaaagctc ggtaaatcaa
      241 tgtggactga aaagcctaaa cggagaacgc cgagaaagat tgcaatctgg catggtgacc
      301 gaattctagt aacaggtact gctgaacagt tatctgaaat tactggtctg agcaaaaaca
      361 ttatctggga tagagctagg agcttatgga ttgattcaaa aggacgacag tttaggtatg
      421 tggaggagag ataatggatc tcattacaca atacagtgac atcatcctca agaaaatcat
      481 gatgaagatt cagaaaga
//
LOCUS       DACDKJ010000236          498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDKJ010000236 DACDKJ010000000
VERSION     DACDKJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.249"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..132
                     /locus_tag="IV328_003118"
     CDS             <1..132
                     /locus_tag="IV328_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8939150.1"
                     /translation="IRSLQREIDDLERTKAVMVNETARKAIDLHIENLRREIHRLEE"
     gene            129..434
                     /locus_tag="IV328_003119"
     CDS             129..434
                     /locus_tag="IV328_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939151.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT          193 a           75 c          124 g          106 t
ORIGIN      
        1 attcgaagcc ttcagcgtga gattgacgat ctggaacgaa caaaggcagt gatggtcaat
       61 gaaacggcga gaaaggcaat cgatttgcac atagagaatt taagaaggga aatccatcga
      121 ttggaggaat gagcgtggat aagaaagcag caatgaaaag aattgctgaa ttaaccaagt
      181 cagaatcttg gcaagaagac aaagaaatag ttgcagaagt ccaaaagctc ggtaaatcaa
      241 tgtggactga aaagcccaaa cggagaacgc cgagaaaaat tgcaatctgg catgatgacc
      301 gaattttagt aacaggtact gctgaacagt tatctgaaat tactggatta agcaaaaaca
      361 ttatctggga tagagctagg agcttatgga ttgattcaaa aggacgacag tttaggtatg
      421 tggaggagag ataatggatc tcattacaca atacagtgac atcatcctca agaaaatcat
      481 gatgaagatt cagaaaga
//
LOCUS       DACDKJ010000237          482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDKJ010000237 DACDKJ010000000
VERSION     DACDKJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.250"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..>482
                     /locus_tag="IV328_003120"
     CDS             24..>482
                     /locus_tag="IV328_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8939152.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYK"
BASE COUNT          186 a           63 c           96 g          137 t
ORIGIN      
        1 ataattcata ggaggagata taagtgttta aaggaaaaac aatattagta actggcggaa
       61 ctggttccat tggaagtgaa attgttcgtc agcttttaaa atatgaacca aaagctatca
      121 gaatttatag tagaggtgaa gaaaaacaat tttatatgca acaagagctc tcaaatcaca
      181 ctaatttgag atttttaatt ggtgatatca gagactctca aaggattgat tatgcatgtc
      241 gtgatgtgga tattattttt catgctgccg caatgaaaca cgttccagca agtgaatata
      301 atcctatgga agctgtaaaa acaaatgtga tgggaactca aaatgtaata gatgcagcga
      361 tcaaaaataa tgtaaaacat tttgtaggta tcagtacaga taaagtagtg agtcctacta
      421 acaccatggg tgcgacaaag ttattgagtg aaaaattaat aatagctgcc aatcagtata
      481 aa
//
LOCUS       DACDKJ010000238          451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDKJ010000238 DACDKJ010000000
VERSION     DACDKJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.251"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..308)
                     /locus_tag="IV328_003121"
     CDS             complement(<1..308)
                     /locus_tag="IV328_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939153.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     G"
BASE COUNT          161 a           92 c           51 g          147 t
ORIGIN      
        1 ccatctaagt cttcttcaga ttcaatttca aatccatggc ttaaataaaa atgttttgca
       61 tatttatttt gactattaac atcaattctt tttatatctt cagtttcaat cagctcaatt
      121 aatatttgac taccatatcg cttaccaata aaatctggat caagaaatag cataactaat
      181 tcttctgcac ttacaccact aaaaccgaca atttctccgt tatcttccca taaataaagt
      241 ttaacctcat ctaagttctc aggaatgatt gctttataaa acgccgtttg tataattaag
      301 cgagacaaat aaaaaagcca tttatgttac actcaaattg taatacccgc taaagcatac
      361 aaggagagtg aacataaatg acttataccc atcttacacc aaacgagctt gtaatgatag
      421 aagcatattt tcatcaagaa actccggttg c
//
LOCUS       DACDKJ010000239          451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDKJ010000239 DACDKJ010000000
VERSION     DACDKJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.252"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IV328_003122"
     CDS             <1..156
                     /locus_tag="IV328_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP8939154.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>451
                     /locus_tag="IV328_003123"
     CDS             175..>451
                     /locus_tag="IV328_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939155.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKL"
BASE COUNT          198 a           35 c           78 g          140 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta a
//
LOCUS       DACDKJ010000240          437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDKJ010000240 DACDKJ010000000
VERSION     DACDKJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.253"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            227..424
                     /locus_tag="IV328_003124"
     CDS             227..424
                     /locus_tag="IV328_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8939156.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          159 a           61 c           78 g          139 t
ORIGIN      
        1 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taataatatt taaaccgtat
       61 atcgagtgat tatccttgtg ttagcattac atccgtgtta tagtaaataa gtaatctaat
      121 ttgaaacgta atctgagcga tatattcaca ctataaaaac tccttttacc aagtaatatt
      181 aattgcaata aaacacgtat tatatacgta tcaggaggaa atatatatga ataacggtac
      241 agtaaaatgg tttaactcag ataaaggttt tggtttcatc actggtgaag atggaaatga
      301 cgtattcgct catttctcag caatccaagg agaaggcttt aagtctttag atgaaggcca
      361 agcagttact tatgatattg aagaaggaca acgtggtcct caagcagtaa atatcgtaaa
      421 ataatgttga acgttaa
//
LOCUS       DACDKJ010000241          425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDKJ010000241 DACDKJ010000000
VERSION     DACDKJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.254"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..193)
                     /locus_tag="IV328_003125"
     CDS             complement(<1..193)
                     /locus_tag="IV328_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8939157.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREE"
BASE COUNT          156 a           70 c           47 g          152 t
ORIGIN      
        1 tttcttctct acctagaagg tagtctacag agactccaaa gtaatcagct aaccgagcta
       61 actcaataga attaggagtt tgtcttttcc aacttcctaa atatccattc gaatacccga
      121 aattcatttc gagctgtcta atggataatc ctttttcttt tgctaactcc tttattattt
      181 cgtaagtatt cattgataaa tcaacctttc tgaatgctta caaaaaaagt ttagataaat
      241 aatgtaaaaa tagttgactt atatacatta ttaatctata ctatgttttg taaacaagtt
      301 taacaactaa aaagacaaca aaaaatacta ttgattaata aatgccaacc gccaagaaag
      361 ctttttaaat caaagtttat atgtcttatt taactatgca tttagtatag attattaatc
      421 taata
//
LOCUS       DACDKJ010000242          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDKJ010000242 DACDKJ010000000
VERSION     DACDKJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.255"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IV328_003126"
     CDS             complement(<1..339)
                     /locus_tag="IV328_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8939158.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACDKJ010000243          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDKJ010000243 DACDKJ010000000
VERSION     DACDKJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.256"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IV328_003127"
     CDS             <1..379
                     /locus_tag="IV328_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8939159.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DACDKJ010000244          401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDKJ010000244 DACDKJ010000000
VERSION     DACDKJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.258"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            124..327
                     /locus_tag="IV328_003128"
     CDS             124..327
                     /locus_tag="IV328_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8939160.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          164 a           41 c           76 g          120 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa t
//
LOCUS       DACDKJ010000245          375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDKJ010000245 DACDKJ010000000
VERSION     DACDKJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.259"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV328_003129"
     CDS             complement(<1..132)
                     /locus_tag="IV328_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8939161.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DACDKJ010000246          363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDKJ010000246 DACDKJ010000000
VERSION     DACDKJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.denovo.260"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>363)
                     /locus_tag="IV328_003130"
     CDS             complement(<1..>363)
                     /locus_tag="IV328_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8939162.1"
                     /translation="KHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIK
                     LPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTL
                     FFNSSFKNKNVIGGPDLP"
BASE COUNT          120 a           48 c           53 g          142 t
ORIGIN      
        1 gtggtaaatc tggtcctcca ataacatttt tatttttgaa cgaggaatta aaaaaaagtg
       61 tatgaaaaaa tttttcatct ggggcaaaca tacctatttt aaaattcagg tagtcttgtt
      121 gattttgttc atacatttgt attaattctg ttaggcattc tttcgacaaa gcccaccatt
      181 gagaaccatg atacaaatca aatttatggt gtctaatttt tgggggaagt ttaatcatcc
      241 tcagacaacg attaattata gctctaaaaa ttttaaatgc agttgtgtta ttcctattaa
      301 ttctaggata atcatgtttt tgatatacat ctatatgttt ataataaaat tctttatgtt
      361 taa
//
LOCUS       DACDKJ010000247        13162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.079, whole genome shotgun sequence.
ACCESSION   DACDKJ010000247 DACDKJ010000000
VERSION     DACDKJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.079"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            404..757
                     /locus_tag="IV328_003131"
     CDS             404..757
                     /locus_tag="IV328_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8939163.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            complement(815..1003)
                     /locus_tag="IV328_003132"
     CDS             complement(815..1003)
                     /locus_tag="IV328_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8939164.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1101..2141)
                     /locus_tag="IV328_003133"
     CDS             complement(1101..2141)
                     /locus_tag="IV328_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8939165.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            2503..3000
                     /gene="dfrG"
                     /locus_tag="IV328_003134"
     CDS             2503..3000
                     /gene="dfrG"
                     /locus_tag="IV328_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8939166.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(3072..5024)
                     /locus_tag="IV328_003135"
     CDS             complement(3072..5024)
                     /locus_tag="IV328_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8939167.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(5031..5327)
                     /locus_tag="IV328_003136"
     CDS             complement(5031..5327)
                     /locus_tag="IV328_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939168.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(5502..6305)
                     /locus_tag="IV328_003137"
     CDS             complement(5502..6305)
                     /locus_tag="IV328_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8939169.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(6321..6437)
                     /locus_tag="IV328_003138"
     CDS             complement(6321..6437)
                     /locus_tag="IV328_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8939170.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(6682..7611)
                     /locus_tag="IV328_003139"
     CDS             complement(6682..7611)
                     /locus_tag="IV328_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8939171.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(7628..8650)
                     /locus_tag="IV328_003140"
     CDS             complement(7628..8650)
                     /locus_tag="IV328_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8939172.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(8647..10674)
                     /locus_tag="IV328_003141"
     CDS             complement(8647..10674)
                     /locus_tag="IV328_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939173.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(10671..13124)
                     /locus_tag="IV328_003142"
     CDS             complement(10671..13124)
                     /locus_tag="IV328_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8939174.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
BASE COUNT         3975 a         2514 c         2215 g         4458 t
ORIGIN      
        1 agtgaccaaa aaagcacata aacagattgt tttctctgtt catatgcttt gatttttcta
       61 tctatgtaat ctgcaaaacc acattgatgg tcatatctat ccagtacagg tggataagtt
      121 tttttgataa gaacttaatt aagagaacta gatgacagat tatctttctc atggcgactt
      181 ttccctccat taagtcgcca attttatcta ctttctagga gtatgactaa attttataaa
      241 gttgtatagg taaataaaat cagcgacctt tctatttgaa attggattca tcgcattaat
      301 aaattccaca gtgttctata ggaactcata aactatataa catgtttttc tatacctgtt
      361 tatggttata taggaacagt aaaatgtata gaggtgattt agtatgcgta aaaaagaaga
      421 taaatacgat tttagagccg ttggtttagc gattaaagag gctcgaatga aacggggtct
      481 cactcgtgaa caagtgggaa caatgattga aattgaccca cgttatttaa caaatataga
      541 aaacaaagga caacacccaa gtacacaagt gctgtatgat cttgtatcat tactccatgt
      601 atctattgat gaatttttct tacctacaga taatttgatt aaaagcactc gaaggttaca
      661 ggtagaaaaa tacatggata gctttacaga caaagaacta tccttaatgg aagcactagc
      721 aaaaggtatc aatgaagcaa gaaatattga aggctagtca gtaaaattca gataaacaaa
      781 aagagccgat aagatgagag tttcatttct caaattatcg gctctgcgtc ttggcgtctg
      841 gctctttgat tgttattata ttcatttttt gaacattaat taaagtttcg ttcttacatt
      901 tggggcagta taaagggaaa tttttaagta tagtatccac tcgtattcgt agtcttgttt
      961 tatttccaca aataggacac aatatccact tgtagtttat aataacaatc tcctcctttc
     1021 cactttaatt caaatctata ttaaagaata tttcatctta tttaataaga aaccatattt
     1081 atataacaac ataaaacgca ctaagttatt ttattgaaca tatatcttac tttatctatc
     1141 cgactattta gacgacgggt ctggcaaaca ggttcgccag tggtaacctg atatcctttt
     1201 agctctgcta aacaaacact aagcccattt gtaaaaaaag ttaaatcatt gcgataatct
     1261 tgaatacatc gagcaggaat ttctccaata ataatgacct cattattttt cagttgagta
     1321 tttacgatat ttgcacaata tttgggagca tcgttatatg cccgtgaaag atattcctgt
     1381 ggtgcataaa ctttaaaact aagatatggc tctaacaatt ctgttccagc ttttctaaag
     1441 gcttgctcca gtacaatagg agtaagcatc cgaaaatctg ctggagtact aacagggcta
     1501 tagtataaac cgtacttaaa acagatttta caatccgtca cattccaacc atataatcct
     1561 tgttcgcaac catagcgtat cccttccata actgcatttt gaaatgattg atttaagtat
     1621 ccaagagaaa ccgagctctc atactgcatt ccacttccca acggaagcgg tgatacagat
     1681 aaaccaatgg aagcccagaa aggatttggc ggcacttcga tgtgaatggt atattctgca
     1741 ttttttaacg gtctctccat ataaatgact gtaggctctt ttagttctat ctccacatga
     1801 tacttttctt gcaacagtgc actaatcact tccatttgta ctttccctaa gaaagaaagt
     1861 ataatttcat gtgtcgtaga atccacgtaa tatcgtagaa gcggatcact atctgagatt
     1921 tccaaaaggg catcaagcaa catttctctc tgttcaggtt tactcggttc aacagttgtt
     1981 tgtagtagag ggtgcggatt ttcaatcttt tttctctgtg gcaatagttt tgtatctcca
     2041 agaacactat ttaacttcaa aaactcattt tgcaaaataa caatttctcc agaataagct
     2101 ctatcaatct tacataattc accatttatt gaagtataca tttctgtaac ttttattttt
     2161 tctttttctg atactctaac cgaatctcgt aaatgtagta ctccactata aaggcgtata
     2221 tatgcaagta agtagttcag ttttggagta caaaatgaca aaatgtactt caaaatggac
     2281 tacttttttc attttaactt acataccaaa ttacatacaa aatgatttat aaaatgcaat
     2341 tgaaaatgat aattgaatga aaatttgtga ttttgtggta ttatgataat ataacttata
     2401 gaaaaaattg cttattcaac taacgggcag gataatgaga aaaggttttt tgatagaaat
     2461 aagaaattag atggtaaatt agttaaaaag gctgtgttat aaatgaaagt ttctttgatt
     2521 gctgcgatgg ataagaatag agtgattggc aaagagaatg acattccttg gaggattccc
     2581 aaggactggg aatatgttaa aaatactaca aagggacatc cgataatatt aggtaggaag
     2641 aaccttgaat caatcggaag agccttacct gacagaagaa atattattct gacgagagat
     2701 aaggggttta cctttaatgg ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta
     2761 tgtaaaaacg aagaagaaat ttttattttc ggaggagaac agatttataa tttgtttttc
     2821 ccttatgttg agaaaatgta catcacaaaa atacatcatg aattcgaagg agatactttt
     2881 tttccagaag tgaattatga ggaatggaat gaggtatttg cccaaaaagg gataaagaat
     2941 gataaaaatc cgtataacta ctattttcat gtatatgaaa gaaaaaactt attgagttaa
     3001 cgggtgcgtt aattgaagaa gaacagtaaa gataaagcac aagagcttgg gattactcca
     3061 agctcttttt gtcatttgaa ataaattata tctttccaat caaattgttt atctgtaatc
     3121 cctaatcttt gagcaggtgt caaaaccttt ttatccgctg atttaaaagg gcgacaaaag
     3181 ttgtagtaag ttcttagaat tgtaatcgca aattgagcat acttcggatt gaagttagcg
     3241 taaatatagc tcttcttatc accacgagct gttgttaatg gtctttcaag agatgatata
     3301 taacgtctta tctgttgaat aaagctattg gtcgaatggt cattaacatt taatagcatc
     3361 tttgcgattt cgtttggttc cagagcagat aaatccgttc tacaatcaac tgaataaaat
     3421 cctttatcct tagtggctaa tggatgtttg atagggttat cggcatattt gcgatatctt
     3481 tctccatctt tactaatagc ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga
     3541 taatgagatg ccaaagtccg tagaaacttt gttttgaatc ctctgatatc tccccaatcg
     3601 agcagttctg cttttgcttg tccgaattct tttaagcatt gttttctgct tttcgtcttg
     3661 ttaacttggc aaataaaatg atgtgcatcg ctcaacctta attctttcgt gaatactcta
     3721 taaaaggcgt ttctaataga gaagtcatca tcactaatca ttcgccattc gtcggcatta
     3781 accatttgtt taattaacca atagtgagca gttgtagtat aaggagcatc aacatttaaa
     3841 ccatcgacaa atctactcct attagtaatt ttccctaatt cgtgaagata agcattccta
     3901 ttttcgctat cgttagcgga tggttcttgt ggataataag accaatctaa cctatcattt
     3961 ttacgactaa aagtattgag atggtcttct ttgaattttc ttgtatcttc atttaattca
     4021 tccattgaaa tattccaatc ataggcaaca tcagaacgga acacatatct tgataagacc
     4081 tcagcagata caactatata cgtttgcata ttaaggtctt caaaccccgt atatttcttt
     4141 gaaccttgtc ctttcttacg gacgttattt aggtagtagt gcatcttgtc cgtattaagc
     4201 cacatctcgt taaatttctt tgtttgaagg ggttgtgtct catatcgttc taaaaactcc
     4261 aaacaacgcc tatacagcca ctccagctta tgatagtagg ttccaactcc aattcctaaa
     4321 atatcacaag tacgactgac tgaaaccctt ccaacaacca tcttagcgaa cattggaaga
     4381 atggtgttct tttgttggtg ataagtagtg gtttcttctc tttttggtag aacattagtg
     4441 aacttcttac acgccttaca ttgatagcgt tgagatttgc ccctactttt cccccttttg
     4501 taaaattgct tcggctcatt gaatggggtt gattcttctt ccgagcagcc ttctttatga
     4561 aattgataat ctggctcaac atcttggatg ctattgattt ctataaggcg tttaatttct
     4621 tcaacaacag accaattaga taaaggggtt actgtacagt tttgcgatac acctctgcca
     4681 ataggattgt cattgcagta cataccttta tgacctttat ctttggaact accagtcatt
     4741 ttatatcgac ttggtttccc ttttacatct ttgaatttgt gctgttcttt cccaaaatta
     4801 caacaaaacg gattgatgca gtaattatat tgaatgggat attcagaacc gttccattta
     4861 aaagacgttg gcaagaacaa cattttgtta taccttgttg caaatcgttt tggagttaat
     4921 aaatcatatt gtttagccct atcttttaat tcttcgtttg aaatcggaat tttaacatct
     4981 acaatttcat cttccttcgt ggctaatctt ttaagcttgg tcattgtcta tcaacccact
     5041 ctatctttga tacccatttt ttcaacaatc cgcttattac ttcgcttgtt ggcaatccat
     5101 tcaataaact tcttatcgtc taaaatgttc aataagaatt catccactgc ttcgctttca
     5161 gtacgctctg catattcagc gtattgctta actatcaccc ttacttgctc tgatatttcc
     5221 caatccacag acttagcgtt cttatttttt ggctcaagaa attgcatagg aaaatgcacc
     5281 cctttttgct tagtaaattg taaccatttt aacacaccaa tttgcattcc tacaataatt
     5341 atttcacgtt cgcattttta tatacatttt aacttacatt ttgtggtgta aattaacaac
     5401 aaacgttgat ttaacaataa aaaacgagta tcaaattcag ttgcaatttg atactcattt
     5461 aggtttacat tttcactttt tgtactccaa aactgaacta cttaatatgc aagacgttgt
     5521 cttttttttg tatattcaat tttgaaaaca tttccgcaaa gttcagacgg acctcgatgt
     5581 gttgatgaat aaaatttatt agtaataact tctataaggt tatcaatccc tatattactt
     5641 tttgcacttc catgataaag agggaacaga gaacaattct gaaatcttat gctttcctct
     5701 tgttcgagtt ccaatgcttc taatgattta ccggacatat atttctctaa aaggtcatcg
     5761 tttccctcta ttaccgtatc ccattgttca gattcggtaa agttcgtcac acacatatta
     5821 ggatacagtt ctaccttctg tttgattaca atttcggcag aaagtttctc tttaatatcc
     5881 tgataaaccg ttgataaatc aattccattt tggtcaatct tattgataaa aaagattgtg
     5941 ggaatcccca ttttcctaag tgcatgaaat aatatacgag tttgtgcttg tacgccatct
     6001 tttgcagaaa tcagtagaat tgccccatct aaaactgata atgaacgata tacttctgct
     6061 aagaaatcca tatgtcctgg cgtgtctatg atgttcacct tcgtattttc ccactgaaaa
     6121 gaggttattc ctgtctgaat tgtaattcct ctctgacgtt ctaaaagcgt attatccgtc
     6181 ctcgttgtac ctttgtccac gcttcctaat tctgtaatcg ctccactgtt atataataag
     6241 ctttctgtta aggtagtttt tcctgcatca acatgagcta aaactccaat attaataatt
     6301 ttcatgtgat tttcctccat tcaaaaaccc aaaagggcat aaaaatccca gtgataaata
     6361 cttttatcac tgggattttt atgcataacc ataggtatac aaagcataca gatattctct
     6421 ggatacttta gaatcacatg ataaaggtat tcttaaactg ggtacaaaaa actaagccct
     6481 cctaaaaaag gacatctaat tatttgttcc cgctatcaaa ttgacagttt atttaagaat
     6541 accttgccgc atatttatta actcctttta aatagtcact taaataatag cacgtaagag
     6601 catatttgta aaggaatctc caatttttta tcaaaaagag tacatgatta caaagtatct
     6661 gtaatcatgt accaatattt gttattttac aatcttccaa ttatcttgtt tttctaatat
     6721 caactcaaat tgcgagattt gggttgcctt tgtttcttga tctaaatatt tcacagcgac
     6781 attcacgatt acttggttat cttttctggt aaagatagga ttcaccaatt cctcgaaaac
     6841 atattccttg ttaatcatag gtaacacatg attacttaca tagtaagtca attctttttc
     6901 tgtggcggta ggatagagct ggaagaacgt ttctaaaaag ctagtgattt cgtccatcgt
     6961 ttctgtatct actgtatggt cactttctag ctgtttgggt tggtagtccg atttttgagg
     7021 tttcttactc attgtcggat ttttgataat aaccatatta tctgattcat ctatatggac
     7081 aaccacatga aagctagacg atatagtttc cttgtctttg tcttcggtaa taacttgttc
     7141 aacagaaaac agtacctcaa aggtgttctc attcacttga gaaacctgcc acacctgtat
     7201 atcgttcaca ctagaactag taggaatatc cttacgaatc atttcttcat ttaatacctg
     7261 caattcttcc gtcaaataat gggtcaattt ttcatttcgt ttatcaatgg cttcttggga
     7321 ttgttgccac gaaaagtagt ctttcgcaaa ggatttcaca aaactttcta cttggttggt
     7381 atcgacaatc tgctgtttaa tgatttctgt ttcacggaca gtatgagtgt caatggcagt
     7441 aaaattttta tagattccaa atccgacact accaatcaat aaaacccata aaacaaaggt
     7501 taatttctta tgcgtgccaa ctttaatcat cggaactttt ctttgcttgc tcggtttttt
     7561 agatttcttt ggtttctcct tcttttcttt tcgctcgatt tttataatca tttgattcat
     7621 tcctttctta gttatgaata acacgtctag ccccaatcag atggtcttgc caataacgac
     7681 tgcttaagtc gccataaccg attgggtcgc ctgcatgata aaagcgattt ccctctaaat
     7741 atatcgccac atgagtcaca tatgtgcctg cattataggt tgaatgaaag aagataaggt
     7801 cgcctgcttg ggcttcttcc atagagattt cttgcgtagc gtcatactga tcttgggcga
     7861 cacgagggag agaaatgcca gctttgtcat acacccattg tattaagcca ctacaatcaa
     7921 aagaggtggt aggcgacgcc cctccaaaga catagggaaa cccttcgtat ttcaaggctt
     7981 cgtccataat gacttgcacg gtttcatcat caaattctgt tggggaagtg tcggttaggt
     8041 attgtgaaac caactgaaca taaaactgat ttccatagtt atatcgccaa ccaccattca
     8101 caggtatggc tataggattg ggatagtctg ctttttgacc acccgacttc tctttagaaa
     8161 actgttcggc taattcatag gtatattttt tcccatgact cctcacataa tttaaaaagc
     8221 caccgccata attataagat tggataacag aatctatatc cactccttgt tgttctgcac
     8281 tggttaataa ttcactgaaa tatttaaccc cttgtttgat ggactcttcg gtactcaaac
     8341 tgttgggcgg taaacccaaa gattcggaac tttgcataac atcttctgct gtaccgcccg
     8401 attccacttg cataatggct aagatataag aaacatagtc ttcaatccca tattccttac
     8461 catatttttc aatcaacgga cggtgtgcca acacttctgg tgaaacggtc acaccgcctt
     8521 gtggaataga agaatccttg ttgttttcgc cactgtccgt atcatctgca aagaaaatga
     8581 ctaggaataa taagagaaaa aagaagagag agaagaccac actacaaatc actgttattt
     8641 ttagcttcat gtttgttgtc ctcgcttatc cgttctttta tttagcttcc tctgattgaa
     8701 ttgcttcggt ggtgtctttt gtttttgctc ttttttcaat ggtcgctgat gattttcacg
     8761 tttagtgttt tgtgacaaaa caggcgtgac ggtttgctga cgcttgcgaa catgctgctg
     8821 gttattctga acaggagatg atgggttttc ttttcgtctt ttttcttttg ctttatccag
     8881 ctccaaacgc ttttgagcaa tgttctttcg tttttgctcc tgctggttca tacgctgttg
     8941 tttgcgttga gctttggtat ctgccataga ttgtttaaag tctgttacat tatgagtgac
     9001 attctctttt ccttgatgaa gggcatattt gacatgggtt ggtgtcgcct gtatctgttg
     9061 ttttgcctgc tttcttttat tgactagacg atttttcgtg tccaatacat gggctgtctt
     9121 ttcacctaat cgttgcccga taccgccttg ttgctttttc acattggaaa gtgctgtttc
     9181 tttctttggt gtagtccgtt cattttgacg agtatggtta gctttattgg tttgattctg
     9241 ctttgttgtc attgtctttt gcttcgttga ccctgctttt ccacgaaaaa tcttactcat
     9301 actgcgttga aatcttctgg tttgtcgatt catcatttga cgaggtctgc gaaacacttg
     9361 tctgcccatg ttttgagagt cgttgctttg taaagtgaat aggctcatta aatcgcccag
     9421 tttaaaataa atccctgcaa aggtcacaat ttgtaaaaac ataatcatga aaaagggaga
     9481 acttgctgac agcgaataaa gcatagtgga aatactaaag gcaacggtaa taatcaaggt
     9541 aataccagcc cttgtcataa tcacattaaa taacttggta atggcatgcc tgcccatgcc
     9601 atcaaaactc ggcaacatac ttaataggaa actgattggc aaaaacatag cgaaaataat
     9661 aaatagcact tgcgaaaaaa tcatgattcc tgttagaagg aaaacaaaaa tcgagatacc
     9721 aatattgaat acaaacaaaa agaagactgt tcctaatctg gaaatcgtct tcggtagggt
     9781 taaataggta ttgtcatgat cttcaatttc ttcaatcact acattttctc tatcttcacc
     9841 attgtttgta tctggactgg tagacaataa actttccaca cgttcttccc ctaacgcttc
     9901 tacatcagtg gagccatatt gaagcaatag ccatggttgc tttacttgaa cagagaataa
     9961 gctgtcacgt atcatgtcca cactgtcttt cccttgacta tccgtgttag gaagaacaat
    10021 ttttgtccca atattcagac tggcggtact gacatctttg gaaaaatcat tgattttcgc
    10081 aatataatcg ggtgcatacg caataaagga agccgataga ataaacacca cgacaaagtt
    10141 taaaacagct cgtatcactt tcgtcgtttc tcgcttaacc aatcctgtat aggcaacata
    10201 gattccaaca atgagaataa aaagaaggag gaaaccgaca taaaaccctg aactggaaaa
    10261 gccattcggt gtaatgcctg caatcgtctg catgttttcg ccaattgctt gggatgtttg
    10321 actaatgaaa tctaaggcat aggcttcttt caccaaataa cctgtggcgt tcgatagata
    10381 aagagaaatt gtccaaataa aattagtaat ggcatacaat ccatacatca ccgattgtcc
    10441 tatgccatca ctccaattcc atggaagcca gccccagcta ttgtccacat aataatcaag
    10501 ctgatattga ttcaaaccat acttggagta ttcattgctt gtatcaatgg tttcatcaac
    10561 taagcctgtc gcttgtactg cattgcccag cgtgcctaat aaaagtaaaa taacgcccac
    10621 acaaaggagc gttatccaac tatagtgaag aaatttcttt tgtttttgtg tcattcttcc
    10681 acctcatttc gcacaggcgg tctggtatca aaggcatgaa gcaactcttc aaaaatcggg
    10741 tgaaactgca taacccctac acgaccatag atgtctgaaa ctaaacattg tccattttct
    10801 aattcacgta agcgtttctg attgttttca tcttccttgt ccacaccaaa aaattctaag
    10861 gtctttttga tttccactag gtcagtggaa cgaaaggcaa atttcaagcc aatattattc
    10921 ttcatctttt catctagtaa atcgtccgcg ttttgagtaa cgaaatagac ccctgcattc
    10981 atggcacgtc ctgctcgaac caatcgcata gatagagctt ttccttgtgc cacttgtaaa
    11041 aaactccaag cctcatcaag gtcaacaatt ttaaaaatgg aacggtcaga gtggataaag
    11101 tctaaagcaa aggtactaat gacaatcagc attgccacac ttaataattc catggtagtg
    11161 tattcttcaa aagagctatt ggaatcgggt aagaccaaat ctgccacttg aataatattg
    11221 agttgttttt ccaagctaat ggattgggtg acactgccat cactgaataa cagatgggca
    11281 aaatcataat cgacaaagct ttcgatatgg tcagcaatac tattgctgat gggcgtatct
    11341 tcttttcgca attcatcaat caccaataac aaccctcgtt gtttgctttg agttacactc
    11401 cgaatagctt tacgcaggac aggaaacttc tcgctatctc ggctggaaat acctgttaag
    11461 aacgtgagaa tatcaatggc aaggctttct gaatctttcg ggagttttaa aatcacataa
    11521 gggtcaagta accctttatt gtcgttctca ctcgtgagat taacaatatt gatttcatgg
    11581 gcaatatcag gtaaggtttc tttccattca ccacgttcag cttttgggtc aacaatggtt
    11641 gcctgtccgc caaagagtac tgaataatag accagcatat tgttagagaa tgattttccg
    11701 ccacctaatg aaccaataaa agcggacgct aatgcatttg ttacagagcc tttaattcct
    11761 tgacttgcaa ggctaggttg gagataaata tttctgcctg tatcgagatt gtaaccgacg
    11821 taaataccag aattttcacc aatcatttgt gtagcaccga aaccaagacc agccaaaaaa
    11881 tccgacgtga catattgaat atagtcattg atatatcgct tgcttgaggg aataaattca
    11941 ttatgcaagc ctagcatatc gccaaaaggt cgtactaatt tgatagacag atcatcataa
    12001 aaatctttta cttcatcaca acgttgtttc agctcgtcca aattctttgc tgatacccgt
    12061 accacatagc ttagtttgta catagattct ttggtttggt ctaaattact ttctaattca
    12121 tcgacagatt ctaaagcgtc caccacatta gtactggttt cgttgtcgct ttcccatgcg
    12181 tggctatcta aatctttcag ttctttcttt ttattgcgaa cggtagataa ggctttttta
    12241 ttggcgacaa tttcaacatt catactggta tcaacgggga aagtaaactg ctgttgctgg
    12301 taataaaaaa tttcactatt agggaaatcc aattcaccaa caatactatt aattgtaaaa
    12361 tacgccacat aagtagtggt atcttcctgt tcaatccgta aatagcgttg attttcttcg
    12421 attaaacagc gagtgggttt gagaatatcg tatttcttta ccaacacttt tttatcttcg
    12481 tatcgtttcg gtaaatggta gtcgtagtca tcaaaaggga cacctgtttg cccgtaaaga
    12541 tgttcaataa tataaccaaa atcatctttc tccaatttac gaatctgaaa gcgtctggat
    12601 aatttacttt ctaacaactg ttccatctta gaaaagcgtt cgatttccgc cataggcata
    12661 gaaacaaaat cgcccattaa tttgtgattc acaccatgaa agaattccgc aaatgctttt
    12721 tgaatttctt tcccaacatt ttttacggat acttcctgtt cattgaggta gagtttaaaa
    12781 ccaataaaaa agcgatagtc tacttgattg tcaccaatca tagaaattaa cgcttcggtc
    12841 tgttcatcta ttttttcgta agctatctct tttaatcgtc cagtgatttc ttttttcgat
    12901 tgttcctgtg tggctcgaat acttgattcg gttgctagtt gcagagcatg aatcttgcca
    12961 tcacgatttt gggcaatcaa ttgacagaaa ttatcatgaa cttgaatttt ttcatcggaa
    13021 cttaaaaaac tgtaattata gggaattaat tcataataag cgaatacttc gccatcatga
    13081 ttaaatacga gattatcttc catatattta atcggatatg ccatctagtt gttcactcct
    13141 tacaatcgta atcgattcat tc
//
LOCUS       DACDKJ010000248         8220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.096, whole genome shotgun sequence.
ACCESSION   DACDKJ010000248 DACDKJ010000000
VERSION     DACDKJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.096"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(134..577)
                     /locus_tag="IV328_003143"
     CDS             complement(134..577)
                     /locus_tag="IV328_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8939175.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IV328_003144"
     CDS             complement(795..2135)
                     /locus_tag="IV328_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8939176.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IV328_003145"
     CDS             2404..3300
                     /locus_tag="IV328_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8939177.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IV328_003146"
     CDS             complement(3450..4391)
                     /locus_tag="IV328_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8939178.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IV328_003147"
     CDS             4517..5638
                     /locus_tag="IV328_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8939179.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IV328_003148"
     CDS             5896..7398
                     /locus_tag="IV328_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8939180.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IV328_003149"
     CDS             7465..7992
                     /locus_tag="IV328_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8939181.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2734 a         1519 c         1619 g         2348 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
//
LOCUS       DACDKJ010000249         7479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.101, whole genome shotgun sequence.
ACCESSION   DACDKJ010000249 DACDKJ010000000
VERSION     DACDKJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.101"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..982
                     /locus_tag="IV328_003150"
     CDS             <1..982
                     /locus_tag="IV328_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8939182.1"
                     /translation="TLFEAFRGKVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYE
                     VNHIANLANASVINKFHELEMPGKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYW
                     MDVYMWGKYPIAAMRFLEEKGWAPTIEAGDAELLESAKPDFLGINYYQTATNAFNPLN
                     GVGAGKMNTTGKKGSSEETGVPGMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRY
                     RMPVLITENGLGEYDKLTEDKQIHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYT
                     DLLSWLNGYQKRYGFVYVDQDETQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1171..3618
                     /locus_tag="IV328_003151"
     CDS             1171..3618
                     /locus_tag="IV328_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8939183.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            3848..4915
                     /gene="rlmN"
                     /locus_tag="IV328_003152"
     CDS             3848..4915
                     /gene="rlmN"
                     /locus_tag="IV328_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8939184.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5108..6211)
                     /locus_tag="IV328_003153"
     CDS             complement(5108..6211)
                     /locus_tag="IV328_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8939185.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            6764..7312
                     /gene="aac(6')"
                     /locus_tag="IV328_003154"
     CDS             6764..7312
                     /gene="aac(6')"
                     /locus_tag="IV328_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8939186.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2512 a         1283 c         1616 g         2068 t
ORIGIN      
        1 aacattattt gaggcattcc gcgggaaagt ccattactgg attagtttga atgaacaaaa
       61 tatcttcact tcattaggtt atttgttagc agcacatccg ccaggtgtga cagacccgaa
      121 acgaatgtat gaagttaacc atattgctaa tttagcaaat gcatctgtca tcaataaatt
      181 tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc caaattatcc
      241 aattgacagt aatcctgaaa atgttctggc tgctgaaaat gcagaagacc tgatggccaa
      301 ttattggatg gatgtctata tgtggggaaa atatccgatt gctgccatga ggttcttaga
      361 agaaaaagga tgggcaccaa cgattgaagc tggcgatgca gaattgctag aatctgctaa
      421 accagatttt cttgggatca attactatca aacggcgacg aatgcattca atccattaaa
      481 cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg
      541 agttcctgga atgtataaaa aagttgagaa tccatttgtc gaacgaacaa attgggactg
      601 ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat
      661 gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca
      721 aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc
      781 aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag
      841 ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca
      901 agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga
      961 agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg
     1021 ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa
     1081 aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat
     1141 aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa
     1201 aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt
     1261 ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta
     1321 ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt
     1381 cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt
     1441 aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat
     1501 gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat
     1561 ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt
     1621 ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca
     1681 gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg
     1741 ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc
     1801 aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa
     1861 gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag
     1921 aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat
     1981 acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct
     2041 gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt
     2101 tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag
     2161 gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat
     2221 caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa
     2281 agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc
     2341 gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc
     2401 gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg
     2461 attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa
     2521 gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga
     2581 cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt
     2641 agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc
     2701 catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc
     2761 atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa
     2821 aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat
     2881 atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca
     2941 accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc
     3001 aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat
     3061 atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat
     3121 gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat
     3181 tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag
     3241 aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt
     3301 caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa
     3361 gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca
     3421 cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt
     3481 tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca
     3541 gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg
     3601 gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa
     3661 agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt
     3721 atgatgtctt gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag
     3781 tattgcatta aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga
     3841 aaatactttg gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt
     3901 tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa
     3961 acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc
     4021 ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac
     4081 agtaaaatat ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca
     4141 agaatatggc ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt
     4201 ttgtgcaagc gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca
     4261 gatcatgatg gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt
     4321 cgttgtaatg gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca
     4381 tatcatcaat gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag
     4441 tggattagca cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat
     4501 ttccttgcac gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt
     4561 cccaattgag aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat
     4621 cacgtttgaa tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt
     4681 agcagatcta ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc
     4741 agtcagtgaa catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga
     4801 tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga
     4861 tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta
     4921 tcctactcgt tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca
     4981 acttaaataa gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa
     5041 ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag
     5101 caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt
     5161 ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa
     5221 tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat
     5281 tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa
     5341 acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc
     5401 cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca
     5461 tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat
     5521 ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt
     5581 gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat
     5641 ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag
     5701 catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa
     5761 tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt
     5821 tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga
     5881 agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac
     5941 cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat
     6001 ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca
     6061 aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta
     6121 cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg
     6181 tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg
     6241 ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc
     6301 taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc
     6361 ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta
     6421 gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat
     6481 tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga
     6541 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
     6601 ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta agataaataa
     6661 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
     6721 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
     6781 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
     6841 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
     6901 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
     6961 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
     7021 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
     7081 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
     7141 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
     7201 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
     7261 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
     7321 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt
     7381 gaatttttgt gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca
     7441 gaggttgtga aaaagtcgct tagtttcgag tagtaagaa
//
LOCUS       DACDKJ010000250         6624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.104, whole genome shotgun sequence.
ACCESSION   DACDKJ010000250 DACDKJ010000000
VERSION     DACDKJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.104"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(162..362)
                     /locus_tag="IV328_003155"
     CDS             complement(162..362)
                     /locus_tag="IV328_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8939187.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(512..655)
                     /locus_tag="IV328_003156"
                     /pseudo
     CDS             complement(512..655)
                     /locus_tag="IV328_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(890..1552)
                     /locus_tag="IV328_003157"
     CDS             complement(890..1552)
                     /locus_tag="IV328_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8939188.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            1916..2003
                     /locus_tag="IV328_003158"
                     /pseudo
     CDS             1916..2003
                     /locus_tag="IV328_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            2061..2792
                     /gene="erm(A)"
                     /locus_tag="IV328_003159"
     CDS             2061..2792
                     /gene="erm(A)"
                     /locus_tag="IV328_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8939189.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2918..3700)
                     /locus_tag="IV328_003160"
     CDS             complement(2918..3700)
                     /locus_tag="IV328_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8939190.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(3854..4231)
                     /locus_tag="IV328_003161"
     CDS             complement(3854..4231)
                     /locus_tag="IV328_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8939191.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(4238..6130)
                     /locus_tag="IV328_003162"
     CDS             complement(4238..6130)
                     /locus_tag="IV328_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8939192.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            complement(6127..>6624)
                     /locus_tag="IV328_003163"
     CDS             complement(6127..>6624)
                     /locus_tag="IV328_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8939193.1"
                     /translation="LRIGEVLSLRLEDIVTWDNQIHLTPRDVNVNEAYIKLRKERTIH
                     VSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFGKPLKYQSVLDLVRRIVKRTGIE
                     FTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTTLNTYVHLSDQDMKNEFNKYLER
                     KEHKK"
BASE COUNT         2056 a         1280 c          934 g         2354 t
ORIGIN      
        1 atacaaggtg gctctttcat agcctcttaa atttatttac tttttcatac aaaaaaatac
       61 ctgccacaag agaaattgtc tcttgtggca ggtatttttg ttactcgctt tgttctctcg
      121 taaaaattga tgtctaccat caatttgatt tacagataaa attaagcttt tacaacgttt
      181 gcagcttgtg ggccgcggtc tgattcttca acgtcgaaag tcactgcttg accttcttct
      241 aatgttttga atccgtcacc ttggatagct gagaagtgaa cgaatacgtc gcttccatct
      301 tcacgtgaga taaatccaaa tcctttttca gcgttgaacc attttactgt tccgttttcc
      361 ataataatat cctcctcgtg ccaaaagcac taatttaaat tgtaacattg ctcgtgtacg
      421 atagaggtgc aacagatgtt tcaacttcta ttcccaaaca aaattacaat ataattgtac
      481 acttgcttca ttttttttgc aagaaagcaa cttacttctc ttccatcatt ccttcttctc
      541 tcaaactaaa atatcgtttg cgtccgatga tcaagtggtc cagtacagtg atccccatca
      601 tttctccaca cttttgtatc cttttggtaa aattcatatc ctgctgcgat ggaatttcat
      661 attatcaaga gtaaattaca aaagaatcat ttaaatcaat actttcactt tgaatacatg
      721 tatttgaagt ggaaggtact tatttcaaaa atagtaaaac ctgtatctta aattacttaa
      781 tagtaacata agatacaggc tgatttttta ttcattgtta tttatactaa agcacccgat
      841 agctctgaaa acaatcacaa attcaacttt tcaaagccac agctttaagt tattttgtcc
      901 agacaacccc cattgcccaa cccattttat ggaattggca tccaggcaac aacttttcat
      961 ataaatcgta ataattttgt tcagataggt acttatctga agctaaatgc tcaagccatg
     1021 atttagatgt gttgtgatta taaattctaa tcgcattttt tattccaaat ttgaaacaat
     1081 gcggtaaaaa ctcttggatt gcccctaatt tataaacata ggtaggcggt gtctcgactt
     1141 cagatacatt atcaagaata actattcttc cttcctcatt caacagttcc ttcatttgct
     1201 gtattacgct ggctatatca tccaaatgat gaaaggttgt tcggcttaca ataaaatcaa
     1261 acttctcatt aaaattaagt tgttctgcat tcatattcag atagaccgta tttgttagtt
     1321 gacgtttaga tttggcaaga tcgagcattt gattagaaat atcaatccct accacttcat
     1381 cataataact tgctaatttc tccactaaca aacccgagcc acatccgata tctaatgctc
     1441 tgcctttctt tggagacata ttagacacaa agaatgaata atcattcaaa agctcattca
     1501 cgaaatcgta atcttctgca accttatcaa actgtgattc tattgtattc aaaaagatcc
     1561 cccattccta ctttatcgac attctttcat tacttaccac tttagatgtt ttttcgttgg
     1621 ggataaaact tccctttaga caattttatc caaagacaat acaacagtgc aactttatta
     1681 aagtcactgt cctttatcgc agcctttact ttttagtaaa gacagtggct tctcttatca
     1741 agtttcaaaa catattattt tgaagaaaac gtccatctga agtgtcaagt gcaaaattac
     1801 atataaaggt ttattctaaa atgaaaagat gatacaatca tattcagtta cataaggagg
     1861 tttcaattat gtgcaccagt atcgcagtag tagaaattac tttatctcat tcataatgaa
     1921 aaaaatggaa aggagataaa agtatgggta ctttttctat atttgttatt aataaagttc
     1981 gttatcaacc aaatcaaaat taattggtta taatgaacgc ttaatgtcag ttcattataa
     2041 ccagtaagga gaaggttata atgaaccaga aaaaccctaa agacacgcaa aattttatta
     2101 cttctaaaaa gcatgtaaaa gaaatattga atcacacgaa tatcagtaaa caagacaacg
     2161 taatagaaat cggatcagga aaaggacatt ttaccaaaga gctagtcaaa atgagtcgat
     2221 cagttactgc tatagaaatt gatggaggct tatgtcaagt gactaaagaa gcggtaaacc
     2281 cctctgagaa tataaaagtg attcaaacgg atattctaaa attttccttc ccaaaacata
     2341 taaactataa gatatatggt aatattcctt ataacatcag tacggatatt gtcaaaagaa
     2401 ttacctttga aagtcaggct aaatatagct atcttatcgt tgagaaggga tttgcgaaaa
     2461 gattgcaaaa tctgcaacga gctttgggtt tactattaat ggtggagatg gatataaaaa
     2521 tgctcaaaaa agtaccacca ctatattttc atcctaagcc aagtgtagac tctgtattga
     2581 ttgttcttga acgacatcaa ccattgattt caaagaagga ctacaaaaag tatcgatctt
     2641 ttgtttataa gtgggtaaac cgtgaatatc gtgttctttt cactaaaaac caattccgac
     2701 aggctttgaa gcatgcaaat gtcactaata ttaataaact atcgaaggaa caatttcttt
     2761 ctattttcaa tagttacaaa ttgtttcact aaattaaagt aataaagcgt tctctaattt
     2821 cacaagagga cgctttattc ttcccaaaaa ttgttcaata tttatcaata aatcagtagt
     2881 tttaaaagta agcacctgtt attgcaataa aattagccta attgagagaa gtttctatag
     2941 aatttttcat atacttaacg agtgctttca cctttgaata tagtccttcc cacttatcat
     3001 cacactctcc ccgatagcct tttctagcta tatccagtaa agttacatgc tctttaggta
     3061 aaagaggtat agcccattct gcagcgacat ctttcgaggt aatttcacca gtagtcactg
     3121 tttgccacat tcgagctagg gttaaaatta cattacgctc atcacctttt atcccctcaa
     3181 ttagttctgg caaagaatcc ttaattgctc ttcgaatatc tgtcaaaggt acggagacaa
     3241 gtatacttga agaatcagga ccaaatagag aaatactatt ctttcttgct tgtgctaaaa
     3301 caatagccaa atcaggatca tagcttggtt cctgaatttg tccattctca aattcacccc
     3361 tgagccactc accgtatata aattctcttt ttggaggata ttgccaaggg acaacttcac
     3421 tcctatttat aaccgtaact tcaagtggtc taacagaatc cgtatttcca atctttcctg
     3481 atatagtcat tagtctttct gttagttttt ttcgagttaa ttgaggtaaa ctatgattca
     3541 cgacgactag aacatctaca tcgctgttaa tgcgtaaacc accatttact gctgaaccaa
     3601 atagatatac tccaactatt gaacttccaa ataaatcttt tacgattttt aatgtttgaa
     3661 tcgcttgatt tggtattttt ccgttaatca aattgctcat gatttcacct cgtaactgat
     3721 tatgttcata taaagtttat attgatactc aatttactta ccctagattg gacatatacc
     3781 taaatgactg ttcaataaag ctgaccgtta gcgtttaagt acatcctttc acaatttgtc
     3841 tacagattaa taattattct ttattataca gatctccata taatttttga atttggttct
     3901 gtaatttttt attttctttt tctaattcca ttactcttct ttttaaggtt ttaataagga
     3961 tttcctccga acgagaactt ttcttgggtt ttgagactac atttgctgtt atttgacgct
     4021 cacgaaggga ttcgattctt tgcctaatat cgtgttcctt ataaagccat gatttagaaa
     4081 cattagcttc ctttgctatt gaattaaaat taataacttt accttcaatc gaaaatttag
     4141 aaatcgcttt gtctactttt tcccttgtct tttttgattt ctgcttcgcc aaacgtacaa
     4201 tttctgttgt atttctaact tgtttatcca ttgataatta ccccgtcaaa cttccaatga
     4261 tttgttctaa acgctcttta acacggctat tagtctctac ttgtctttgc cattgtttat
     4321 ccttagctat ggctaataac tcttctgtac gctctaactg ttcttcgtgc tgtggtaaga
     4381 attgcttact ggtacagaag tgagtgcaat ctaagcatgc attcgcatgt ggacaaccac
     4441 ctgctactac tggcaatcta caataaccat ttggaagcac ttgtgcattt atatttttct
     4501 tgaaccattg aagctctaca tcatcgactt cattatcttc atctagatca agcacatctc
     4561 cattattggt aaccagtttt tcctgaaatt tagtaaattc attttttaga gtttcatcaa
     4621 agatatgagc gtatctgctt gtcatttctg ggctttcatg ccccaaaaat ttctgcacaa
     4681 tatgctgggg catcccgttg ttaatcattc ttgttcctac tgtatggcga aaggcatggg
     4741 catggaatct ataaatctca cctgatttat ccactatatt ttgctcataa gctaatttat
     4801 ttaactcacc tctaaatgtt tcttgtttta atggcgatcc atcttttctt ggaaagaggt
     4861 attcactatc tggaaattcc tctgaaactt tatcttcccg aactttaata agtaaagcta
     4921 cctctttaga tattggaact atatgctcct ttttcatttt ccattgataa tactttaaaa
     4981 agaaatctcc atctttgtcc tctaatagac agcctttttt caaggtgcac aattcactta
     5041 tcctcattcc acattcttga acaatcatag tcatcgtagc tatatattcg ggtaatttat
     5101 caagatgact gttcaattgc tctaggacga attcatctat aaagcgtggt tttgctcttg
     5161 gtattttcgg atagtcctca gaataaatta atattttgga aggaacatca tcccattcta
     5221 gcctaagaag ggtactaaat agtccttcca atatagagat cctcccagtt attgtactag
     5281 gttttattcc catcatgttt agttcactta aatatgcttc aatttccact ctcgttaatt
     5341 ggtgtactct ctgaacttgt ttaaatttca tgtccagaaa attaaagaac tctttaagtc
     5401 tttgggcaat atcacttaca taggaaaagc tatccatgtt caatctcaac ttacaatatc
     5461 tttttacaag ttgtttaaaa tatgtattcc gaaacccttt aaagttaatt gtatattcat
     5521 attgtgttgg gttaacctta tcatctggca aaggtaagtt acgtctatcc caaacgtctt
     5581 tatcccactc ctctccatca aaataaaagt tctcataaaa ctccataaat tgttttagat
     5641 tagtaacata gtaggaatta gcttttacag gtgttttttc ttgattagca gtaatcttat
     5701 aattagtagt ggtaattcta acaccccgtt ttgtcaaata agttctatac tccgtcattg
     5761 ctttttcaat aggaacttca gtaattgaag taatgctagg atactttaaa tctaagaaat
     5821 ctaacatttt attaattact gttccttttc taatccagac agtttttgca ttccatattc
     5881 cattgtttaa atggtaaaaa taaaaatatt tcaattctgt tcttaaccac agatttttaa
     5941 cacgttcaaa acgaacccaa cgattcctta aagcaggatt cttacttaat tctatggcag
     6001 aaggatgtgg acattttctt atatcccaac tattattagc ccaaaacccc tgcatttctt
     6061 cattcattac agctattttt ttgctaatct cactctgact aataattttc cttttactag
     6121 aagcattcat ttcttatgct cctttctctc gaggtattta ttaaactcat ttttcatatc
     6181 ctgatctgaa agatgaacat aggtatttaa cgttgtctga acatgtgcgt gacctaatct
     6241 cttttgaacg aacgcaacat cccatccttc cctaattagc tgcgttgcgt gagtgtggcg
     6301 aagcatatgt gatgtaaatt ctattccagt ccttttaact attcttctaa ctagatcaag
     6361 aacactttgg tactttagtg gtttcccaaa atagccttct tttaaggaaa taaaaacata
     6421 atcatgctcc aattcctcac tatactcata tatcaagtaa tctgtataaa gtgacataag
     6481 ttctttactc acatgtattg ttctttcctt ccttaattta atataagctt cattaacatt
     6541 aacatctcta ggtgttaaat ggatttgatt gtcccaagtg acaatatctt caagcctaag
     6601 cgataacact tcaccgattc ttaa
//
LOCUS       DACDKJ010000251         5985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.110, whole genome shotgun sequence.
ACCESSION   DACDKJ010000251 DACDKJ010000000
VERSION     DACDKJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.110"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(40..1026)
                     /locus_tag="IV328_003164"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="IV328_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="IV328_003165"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="IV328_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8939194.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="IV328_003166"
     CDS             1851..3005
                     /locus_tag="IV328_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8939195.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="IV328_003167"
     CDS             3220..4188
                     /locus_tag="IV328_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8939196.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="IV328_003168"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="IV328_003168"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8939197.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5826
                     /locus_tag="IV328_003169"
     CDS             5218..5826
                     /locus_tag="IV328_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8939198.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1161 c         1347 g         1666 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
     5581 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
     5641 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
     5701 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
     5761 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
     5821 aaataaactt ttaaccgttg cacggacaaa ctatataagc taactctttc ggcaggaaac
     5881 ccgacgtatg taactggttc ttagggaatt tatatatagt agatagtatt gaagatgtaa
     5941 ggcagagggt tctgttgcaa agttttaaat aaagaataaa atccc
//
LOCUS       DACDKJ010000252         4916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.120, whole genome shotgun sequence.
ACCESSION   DACDKJ010000252 DACDKJ010000000
VERSION     DACDKJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.120"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..512
                     /locus_tag="IV328_003170"
     CDS             <1..512
                     /locus_tag="IV328_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8939199.1"
                     /translation="APSSVNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMI
                     FGDMQCYEYGEEIYNQAYESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLA
                     AMQFMLVARDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRL
                     APEKITMFE"
     gene            866..2344
                     /gene="msr"
                     /locus_tag="IV328_003171"
     CDS             866..2344
                     /gene="msr"
                     /locus_tag="IV328_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8939200.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2391..2837
                     /locus_tag="IV328_003172"
     CDS             2391..2837
                     /locus_tag="IV328_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8939201.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3036..3551)
                     /locus_tag="IV328_003173"
     CDS             complement(3036..3551)
                     /locus_tag="IV328_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8939202.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3668..4870
                     /locus_tag="IV328_003174"
     CDS             3668..4870
                     /locus_tag="IV328_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8939203.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1487 a          824 c         1069 g         1536 t
ORIGIN      
        1 ctgcaccttc ttctgtcaat atgaaaccat ggcgctttgt ggtcgctgaa agcgaggaag
       61 caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat acttcttctg
      121 caatggtcat gatttttggc gatatgcaat gttacgaata tggcgaagaa atctacaacc
      181 aagcttacga atcagggaag atgccaaaag aagtcagaga tcaacaatta gctgcgatca
      241 ttccttatta caagagtttt tcaagagaag aaatgaacga tgtagtcaaa gtcgattcta
      301 gcttagcggc tatgcaattc atgttagttg cgcgtgatca tggctatgaa acaaatccaa
      361 ttggtggatt cgaagcagat cagttggcag aggcatttgg tttggataaa gaccgttatg
      421 ttccagttat tattttatca ataggaaaag cggtagaaga aggttacgag tctgttcgtt
      481 tagcaccaga aaagatcact atgttcgaat aaaaggtttt cagttactat tcagtagcta
      541 ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa aaatgactgc
      601 atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa aagacatgta
      661 taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta gaataggtga
      721 aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag gaaaagatgt
      781 gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata attgttgttt
      841 tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa caaatctaca
      901 agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct atcagcaaga
      961 tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca aactaatagc
     1021 cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca actacctgcc
     1081 tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca gtcatttcca
     1141 gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac gaaagatcga
     1201 gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc caacaaccca
     1261 tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt atggtacgct
     1321 ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa tctgggaagt
     1381 aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca ctcaaaagga
     1441 attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga aaaaacgatt
     1501 ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag tgtccagtaa
     1561 aaagaaaaag caacaaatca gactggatcg gttgtcttcc tctaaacaaa aagacagtgt
     1621 acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa aaataggaga
     1681 aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat ctcttgaact
     1741 ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg ggagaacatt
     1801 actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg tcggtgaaaa
     1861 tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag gaatccttct
     1921 ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga catctgaaga
     1981 atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg tccgtagttt
     2041 gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta cattaagtgg
     2101 gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta atgtgttgct
     2161 gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag agaagctaat
     2221 gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg tcgagcgtac
     2281 ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta cgagaaattt
     2341 ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga ttgcctacaa
     2401 ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc ttggggatga
     2461 gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg attggtctag
     2521 tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg ctggcgaaaa
     2581 aaattgcctt taaactacca gtaaatccag aaagcaaggt ccaaatgatc ttaactattt
     2641 catgcttgat ggttttagga atggtgacat gtatgtcttt attcggtgtg atcatggaac
     2701 aaggaatacc tgatcaatta ttctctgctt atatgaatgc ttggaaaatg aatgtgattg
     2761 ccgctttacc gttgcagttg atcatcgttg gcccgctttc aagaagtgtg ctaaaagcaa
     2821 ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa ggccttggca tgctttttgt
     2881 catagtctct tgaatgcagt tctagaaaaa tagtgatccg aacgataggt tttggcctgt
     2941 cgttcggatc gctttttctt ttccaactgt attgctcttt tattgaagat aatttgctct
     3001 tcaatagata tttctagaaa ggttctgttt atcccttact caaaaaactg ctgcatttgt
     3061 tctcttactt gctgacacca ttggagttcc tgctgacaaa gcataattct gcgctggatc
     3121 acaacataac gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa ttttttttga
     3181 tttttttgca tgattttgtt cagctgttgt tctctagtga atagcagtcc agtagctgtt
     3241 gttttatcga gcgtcgaaaa agcatagatt tttgctagaa attcatcttt ttgcgtagga
     3301 gcgggagttt cctctttgat ccattcctct acaagagcac gtccttcatc agtcaagtgg
     3361 tagatctttt tttgagagtg ctcgtctttg tccaaaacgg tcacataacc ctgctggtct
     3421 aacttggcaa gcgttgtata gatctgactg tgatgggctt cccaaaaaat tgtgatcagc
     3481 tgcttcaatt catacccaga acgtggtttg gataaaagca cgcataataa tatataggct
     3541 aaagtattca taaaaatcct acctctcgtc ttattttctt tattatatca cagtaaagtt
     3601 tccacgtttt acatgtgcat attgacatgt aaaataaatc atgtatgatg ctaatagaga
     3661 gaaagggatg gaaatattgg agaatcaaat agatatacaa gaacaaacaa atgaagaaac
     3721 aaaggggaaa agttcagcgc tagttgtagc agctacctgt atgatcgctt ttatgggagt
     3781 gggtctagta gacccaatct taaaaacgat tgctgcacag ttaaacgcta cgccagctga
     3841 aacgaccctg ttatttacta gttacatgtt agtcactggt gtggtgatgc tatttacagg
     3901 tttcttatct agtcggatcg gtttgaaaaa aacattgatg acaggattgt ttatcatcgt
     3961 tgtattcgct ggtcttggcg gattatctgg gaatatcgga atgctgattg ggttacgtgc
     4021 cggctggggt gtcgggaatg ctttatttgt ctctactgct ttagcaacga tcgtcagtat
     4081 tttagttggt aacacagaaa aagcaatcat gatgtatgaa gcagcacttg gctgcggaat
     4141 ggctgttggc cctctagtcg gtggggtctt gggcagtggt tcatggcgtt atccattttt
     4201 tggggtagca tttttgatgt tttgcggttt tatagcccta gcggtacttt tacctgaaac
     4261 tgagaaacca aagagaaaag taggcctttg ggaaggtgtc aaagcgttaa gcaatcataa
     4321 acttcgttca gttggactaa tcgcattgtt atataatttc ggattcttta ctttacttgc
     4381 gtatgcgcca tttttattga ttggctattc tgaattagaa gtcggttttg tattttttgg
     4441 ctggggtgtg cttctggcta ttgcttcgat tttcgttgca ccggctcttg agcggaagtt
     4501 tacgacttat cggacaattt tagcagcatt tgtcttattc attatttgtt tgctgttatt
     4561 aggagctgga acttctatgc cagtccttgt accgatcagc attgtgttag caggattttt
     4621 ccaaggaatt atcaatacac tgttgacgac gattgctatg gaaataccag gtctgcaacg
     4681 aaacgttgct tcgtccagct atagttttgt ccgctttttc ggtggagctt tagcaccatt
     4741 tattgcaggt gtgattggtg aacatattgg tgaaagctat gcgttttatt ttgcaggtgc
     4801 cattgttctt atcagtgtat gttgtatcat ttatcaccgt aattattttg ctacaaatga
     4861 agaagaataa gaaatcagag gttgtgaaaa agctgtcctg catcaagtca taagaa
//
LOCUS       DACDKJ010000253         3938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.139, whole genome shotgun sequence.
ACCESSION   DACDKJ010000253 DACDKJ010000000
VERSION     DACDKJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.139"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            313..2199
                     /locus_tag="IV328_003175"
     CDS             313..2199
                     /locus_tag="IV328_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8939204.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2211..3158
                     /locus_tag="IV328_003176"
     CDS             2211..3158
                     /locus_tag="IV328_003176"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8939205.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3178..3705
                     /locus_tag="IV328_003177"
     CDS             3178..3705
                     /locus_tag="IV328_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8939206.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3768..>3938)
                     /locus_tag="IV328_003178"
     CDS             complement(3768..>3938)
                     /locus_tag="IV328_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8939207.1"
                     /translation="HSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLG
                     AGFMYFSVLFFT"
BASE COUNT         1393 a          676 c          848 g         1021 t
ORIGIN      
        1 cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga atatcaattt
       61 gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat ttgcgtttcg
      121 agcaaagtca aaaaaacgag tccaataaaa taaaaaggta aaaattttca agttttataa
      181 aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga gaacacaaaa
      241 agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca atatccaaga
      301 taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac ttatggtgaa
      361 aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat cgggttgatt
      421 gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat cgatagcagc
      481 gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta tctttctcaa
      541 gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg aaacagcccc
      601 ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga aaacggagaa
      661 gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa agaagatgct
      721 tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag tgaattagat
      781 aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc gcaagtgttg
      841 attgatgaac ccgatttact attactagac gaaccaacaa accatttaga ttatgaagcg
      901 atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat ggttacccac
      961 gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt tggaaaatta
     1021 tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg tgatcgtgta
     1081 gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc ttggatgagg
     1141 gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt cgaagatcta
     1201 aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct agcgacacaa
     1261 cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa tgaccaaaca
     1321 ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat tactggtaaa
     1381 aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc cctggatagc
     1441 ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga aaatgaagaa
     1501 atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga agcaaaaaca
     1561 gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt tccaagattt
     1621 atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt atttttgttg
     1681 aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa tgatttggac
     1741 atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc tgtgatcact
     1801 gtttcccatg accgttattt cttggacaag gtagcagata agttattagt attccaagga
     1861 aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa gcaaaaagag
     1921 acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc tgctgaaaag
     1981 aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga gattgcagaa
     2041 cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga taatttcact
     2101 cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga agaaaaaatg
     2161 gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg atggaacaag
     2221 cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa gacagaacag
     2281 gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa aaagggtttc
     2341 ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg ctttggtttt
     2401 taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt tgggatgaat
     2461 gggcatttga acgctacatc aaaagtccag actataatgg accagatatg actgattttg
     2521 gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag agcaagcttt
     2581 tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat cttggacaca
     2641 tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc gatcaaatca
     2701 aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc gtttctgcat
     2761 ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg tttcaatttt
     2821 atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat gtgtttctag
     2881 gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca catgaaacag
     2941 gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat atcaatcata
     3001 tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact ttagttttga
     3061 atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta gaaggatatg
     3121 acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga tgagaaaatg
     3181 ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga ccgtcttcct
     3241 tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa tacactagtg
     3301 atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg tcagacgatt
     3361 gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca tagtgtggaa
     3421 gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg agggtcagct
     3481 gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat ccatcatgta
     3541 ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact gatcaatact
     3601 agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac gtatcagcgt
     3661 aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag ctatggatag
     3721 actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta agtaaagaat
     3781 aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca aacaacatgc
     3841 atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga aagcccgcct
     3901 gctaccatca aaataacacc agtcattcca agagagtg
//
LOCUS       DACDKJ010000254         1781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.173, whole genome shotgun sequence.
ACCESSION   DACDKJ010000254 DACDKJ010000000
VERSION     DACDKJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.173"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..416
                     /locus_tag="IV328_003179"
     CDS             24..416
                     /locus_tag="IV328_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939208.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IV328_003180"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IV328_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP8939209.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DACDKJ010000255         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.183, whole genome shotgun sequence.
ACCESSION   DACDKJ010000255 DACDKJ010000000
VERSION     DACDKJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.183"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IV328_003181"
                     /pseudo
     CDS             262..373
                     /locus_tag="IV328_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IV328_003182"
     CDS             394..477
                     /locus_tag="IV328_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8939210.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IV328_003183"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IV328_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8939211.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDKJ010000256         1350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.198, whole genome shotgun sequence.
ACCESSION   DACDKJ010000256 DACDKJ010000000
VERSION     DACDKJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.198"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..298
                     /locus_tag="IV328_003184"
                     /pseudo
     CDS             47..298
                     /locus_tag="IV328_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="IV328_003185"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="IV328_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8939212.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DACDKJ010000257         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20826_2016
            SAMN12640358-rid9891683.guided.209, whole genome shotgun sequence.
ACCESSION   DACDKJ010000257 DACDKJ010000000
VERSION     DACDKJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640358
            Sequence Read Archive: SRR10040896
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742840.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:15:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,122
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 18 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640358-rid9891683.guided.209"
                     /isolate="E20826_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            260..>1195
                     /gene="vanY"
                     /locus_tag="IV328_003186"
     CDS             260..>1195
                     /gene="vanY"
                     /locus_tag="IV328_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8939213.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          174 c          238 g          357 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt ggttctgttg caaagtttta aatctactat caaat
//
